LOCUS       DAZKAO010000001        68174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000001 DAZKAO010000000
VERSION     DAZKAO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.001"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1042
                     /locus_tag="ACG8H8_000001"
     CDS             161..1042
                     /locus_tag="ACG8H8_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8985115.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1055..2665
                     /locus_tag="ACG8H8_000002"
     CDS             1055..2665
                     /locus_tag="ACG8H8_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985116.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2742..3401)
                     /locus_tag="ACG8H8_000003"
     CDS             complement(2742..3401)
                     /locus_tag="ACG8H8_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8985117.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3684..4349)
                     /locus_tag="ACG8H8_000004"
     CDS             complement(3684..4349)
                     /locus_tag="ACG8H8_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP8985118.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4496..5101)
                     /gene="dhaL"
                     /locus_tag="ACG8H8_000005"
     CDS             complement(4496..5101)
                     /gene="dhaL"
                     /locus_tag="ACG8H8_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP8985119.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5129..6139)
                     /gene="dhaK"
                     /locus_tag="ACG8H8_000006"
     CDS             complement(5129..6139)
                     /gene="dhaK"
                     /locus_tag="ACG8H8_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP8985120.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6114..6509)
                     /gene="dhaM"
                     /locus_tag="ACG8H8_000007"
     CDS             complement(6114..6509)
                     /gene="dhaM"
                     /locus_tag="ACG8H8_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP8985121.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6663..6983
                     /locus_tag="ACG8H8_000008"
     CDS             6663..6983
                     /locus_tag="ACG8H8_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP8985122.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7095..7952)
                     /locus_tag="ACG8H8_000009"
     CDS             complement(7095..7952)
                     /locus_tag="ACG8H8_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP8985123.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(8060..9364)
                     /locus_tag="ACG8H8_000010"
     CDS             complement(8060..9364)
                     /locus_tag="ACG8H8_000010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8985124.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9361..9870)
                     /locus_tag="ACG8H8_000011"
     CDS             complement(9361..9870)
                     /locus_tag="ACG8H8_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985125.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10045..12180)
                     /locus_tag="ACG8H8_000012"
     CDS             complement(10045..12180)
                     /locus_tag="ACG8H8_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP8985126.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12263..13918)
                     /locus_tag="ACG8H8_000013"
     CDS             complement(12263..13918)
                     /locus_tag="ACG8H8_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8985127.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13920..14234)
                     /locus_tag="ACG8H8_000014"
     CDS             complement(13920..14234)
                     /locus_tag="ACG8H8_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8985128.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14404..14664
                     /locus_tag="ACG8H8_000015"
     CDS             14404..14664
                     /locus_tag="ACG8H8_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP8985129.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14678..14992
                     /locus_tag="ACG8H8_000016"
     CDS             14678..14992
                     /locus_tag="ACG8H8_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP8985130.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15722..16276)
                     /locus_tag="ACG8H8_000017"
     CDS             complement(15722..16276)
                     /locus_tag="ACG8H8_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP8985131.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16402..18331)
                     /locus_tag="ACG8H8_000018"
                     /pseudo
     CDS             complement(16402..18331)
                     /locus_tag="ACG8H8_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18637..19353)
                     /locus_tag="ACG8H8_000019"
     CDS             complement(18637..19353)
                     /locus_tag="ACG8H8_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP8985132.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19354..21177)
                     /gene="glpO"
                     /locus_tag="ACG8H8_000020"
     CDS             complement(19354..21177)
                     /gene="glpO"
                     /locus_tag="ACG8H8_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP8985133.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21191..22687)
                     /gene="glpK"
                     /locus_tag="ACG8H8_000021"
     CDS             complement(21191..22687)
                     /gene="glpK"
                     /locus_tag="ACG8H8_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP8985134.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22823..24283)
                     /locus_tag="ACG8H8_000022"
     CDS             complement(22823..24283)
                     /locus_tag="ACG8H8_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985135.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24273..25568)
                     /locus_tag="ACG8H8_000023"
     CDS             complement(24273..25568)
                     /locus_tag="ACG8H8_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8985136.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25900..26577)
                     /locus_tag="ACG8H8_000024"
     CDS             complement(25900..26577)
                     /locus_tag="ACG8H8_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP8985137.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26607..27698)
                     /locus_tag="ACG8H8_000025"
     CDS             complement(26607..27698)
                     /locus_tag="ACG8H8_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP8985138.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27799..28233)
                     /locus_tag="ACG8H8_000026"
     CDS             complement(27799..28233)
                     /locus_tag="ACG8H8_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP8985139.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28817..30274)
                     /locus_tag="ACG8H8_000027"
     CDS             complement(28817..30274)
                     /locus_tag="ACG8H8_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8985140.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30271..32166)
                     /locus_tag="ACG8H8_000028"
     CDS             complement(30271..32166)
                     /locus_tag="ACG8H8_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8985141.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32224..33999)
                     /locus_tag="ACG8H8_000029"
     CDS             complement(32224..33999)
                     /locus_tag="ACG8H8_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP8985142.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34049..35545)
                     /locus_tag="ACG8H8_000030"
     CDS             complement(34049..35545)
                     /locus_tag="ACG8H8_000030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8985143.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35739..37361)
                     /locus_tag="ACG8H8_000031"
     CDS             complement(35739..37361)
                     /locus_tag="ACG8H8_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP8985144.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37689..39002)
                     /locus_tag="ACG8H8_000032"
     CDS             complement(37689..39002)
                     /locus_tag="ACG8H8_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP8985145.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39247..39471)
                     /locus_tag="ACG8H8_000033"
     CDS             complement(39247..39471)
                     /locus_tag="ACG8H8_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985146.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39491..39913)
                     /locus_tag="ACG8H8_000034"
     CDS             complement(39491..39913)
                     /locus_tag="ACG8H8_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8985147.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40055..40765)
                     /locus_tag="ACG8H8_000035"
     CDS             complement(40055..40765)
                     /locus_tag="ACG8H8_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP8985148.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40783..41280)
                     /locus_tag="ACG8H8_000036"
     CDS             complement(40783..41280)
                     /locus_tag="ACG8H8_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985149.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41317..41769)
                     /locus_tag="ACG8H8_000037"
     CDS             complement(41317..41769)
                     /locus_tag="ACG8H8_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8985150.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41904..42509)
                     /locus_tag="ACG8H8_000038"
     CDS             complement(41904..42509)
                     /locus_tag="ACG8H8_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP8985151.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42959..43579)
                     /locus_tag="ACG8H8_000039"
     CDS             complement(42959..43579)
                     /locus_tag="ACG8H8_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8985152.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43581..44450)
                     /locus_tag="ACG8H8_000040"
     CDS             complement(43581..44450)
                     /locus_tag="ACG8H8_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP8985153.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44473..44880)
                     /locus_tag="ACG8H8_000041"
     CDS             complement(44473..44880)
                     /locus_tag="ACG8H8_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP8985154.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44899..46188)
                     /locus_tag="ACG8H8_000042"
     CDS             complement(44899..46188)
                     /locus_tag="ACG8H8_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8985155.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46215..46517)
                     /locus_tag="ACG8H8_000043"
     CDS             complement(46215..46517)
                     /locus_tag="ACG8H8_000043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8985156.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46542..48635)
                     /locus_tag="ACG8H8_000044"
     CDS             complement(46542..48635)
                     /locus_tag="ACG8H8_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8985157.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49124..49888)
                     /locus_tag="ACG8H8_000045"
     CDS             complement(49124..49888)
                     /locus_tag="ACG8H8_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP8985158.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49999..50868)
                     /gene="mdcB"
                     /locus_tag="ACG8H8_000046"
     CDS             complement(49999..50868)
                     /gene="mdcB"
                     /locus_tag="ACG8H8_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP8985159.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50893..51969)
                     /locus_tag="ACG8H8_000047"
     CDS             complement(50893..51969)
                     /locus_tag="ACG8H8_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP8985160.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51993..52586)
                     /locus_tag="ACG8H8_000048"
     CDS             complement(51993..52586)
                     /locus_tag="ACG8H8_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP8985161.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52590..54266)
                     /gene="mdcD"
                     /locus_tag="ACG8H8_000049"
     CDS             complement(52590..54266)
                     /gene="mdcD"
                     /locus_tag="ACG8H8_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP8985162.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54256..54555)
                     /locus_tag="ACG8H8_000050"
     CDS             complement(54256..54555)
                     /locus_tag="ACG8H8_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP8985163.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54657..56303)
                     /gene="mdcA"
                     /locus_tag="ACG8H8_000051"
     CDS             complement(54657..56303)
                     /gene="mdcA"
                     /locus_tag="ACG8H8_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP8985164.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56310..57221)
                     /locus_tag="ACG8H8_000052"
     CDS             complement(56310..57221)
                     /locus_tag="ACG8H8_000052"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP8985165.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57341..58198
                     /locus_tag="ACG8H8_000053"
     CDS             57341..58198
                     /locus_tag="ACG8H8_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8985166.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58385..58747)
                     /locus_tag="ACG8H8_000054"
     CDS             complement(58385..58747)
                     /locus_tag="ACG8H8_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP8985167.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58936..59118)
                     /locus_tag="ACG8H8_000055"
     CDS             complement(58936..59118)
                     /locus_tag="ACG8H8_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985168.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59093..60532
                     /locus_tag="ACG8H8_000056"
     CDS             59093..60532
                     /locus_tag="ACG8H8_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985169.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60539..63091)
                     /locus_tag="ACG8H8_000057"
     CDS             complement(60539..63091)
                     /locus_tag="ACG8H8_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP8985170.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63205..64536)
                     /locus_tag="ACG8H8_000058"
     CDS             complement(63205..64536)
                     /locus_tag="ACG8H8_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8985171.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64502..65980)
                     /locus_tag="ACG8H8_000059"
     CDS             complement(64502..65980)
                     /locus_tag="ACG8H8_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8985172.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66285..68120)
                     /gene="tdc"
                     /locus_tag="ACG8H8_000060"
     CDS             complement(66285..68120)
                     /gene="tdc"
                     /locus_tag="ACG8H8_000060"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP8985173.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19382 a        14542 c        11668 g        22582 t
ORIGIN      
        1 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
       61 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
      121 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
      181 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      241 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      301 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      361 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      421 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      481 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      541 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      601 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      661 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      721 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      781 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      841 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      901 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      961 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     1021 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     1081 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1141 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1201 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1261 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1321 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1381 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1441 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1501 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1561 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1621 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1681 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1741 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1801 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1861 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1921 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1981 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     2041 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     2101 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2161 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2221 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2281 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2341 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2401 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2461 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2521 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2581 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2641 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2701 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2761 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2821 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2881 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2941 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     3001 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     3061 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3121 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3181 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3241 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3301 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3361 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3421 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3481 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3541 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3601 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3661 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3721 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3781 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3841 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3901 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3961 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     4021 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     4081 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4141 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4201 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4261 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4321 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4381 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4441 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4501 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4561 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4621 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4681 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4741 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4801 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4861 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4921 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4981 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     5041 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     5101 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5161 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5221 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5281 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5341 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5401 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5461 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5521 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5581 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5641 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5701 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5761 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5821 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5881 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5941 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     6001 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     6061 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6121 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6181 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6241 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6301 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6361 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6421 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6481 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6541 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6601 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6661 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6721 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6781 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6841 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6901 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6961 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     7021 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     7081 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7141 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7201 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7261 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7321 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7381 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7441 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7501 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7561 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7621 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7681 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7741 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7801 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7861 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7921 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7981 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     8041 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     8101 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8161 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8221 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8281 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8341 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8401 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8461 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8521 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8581 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8641 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8701 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8761 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8821 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8881 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8941 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     9001 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     9061 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9121 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9181 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9241 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9301 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9361 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9421 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9481 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9541 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9601 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9661 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9721 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9781 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9841 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9901 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9961 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
    10021 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
    10081 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10141 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10201 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10261 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10321 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10381 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10441 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10501 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10561 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10621 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10681 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10741 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10801 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10861 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10921 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10981 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    11041 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    11101 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11161 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11221 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11281 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11341 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11401 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11461 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11521 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11581 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11641 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11701 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11761 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11821 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11881 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11941 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    12001 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    12061 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12121 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12181 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12241 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12301 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12361 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12421 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12481 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12541 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12601 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12661 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12721 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12781 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12841 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12901 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12961 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    13021 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    13081 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13141 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13201 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13261 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13321 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13381 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13441 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13501 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13561 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13621 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13681 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13741 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13801 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13861 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13921 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13981 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    14041 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    14101 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14161 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14221 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14281 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14341 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14401 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14461 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14521 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14581 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14641 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14701 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14761 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14821 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14881 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14941 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    15001 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    15061 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15121 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15181 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15241 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15301 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15361 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15421 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15481 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15541 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15601 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    15661 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15721 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15781 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15841 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15901 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15961 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    16021 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    16081 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16141 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16201 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16261 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16321 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    16381 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16441 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16501 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16561 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16621 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16681 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    16741 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16801 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16861 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    16921 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    16981 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    17041 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    17101 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    17161 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    17221 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    17281 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    17341 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    17401 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    17461 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    17521 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    17581 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    17641 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    17701 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    17761 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    17821 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    17881 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    17941 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    18001 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    18061 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    18121 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    18181 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    18241 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    18301 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    18361 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    18421 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    18481 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    18541 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    18601 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    18661 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    18721 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    18781 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    18841 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    18901 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    18961 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    19021 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    19081 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    19141 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    19201 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    19261 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    19321 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    19381 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    19441 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    19501 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    19561 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    19621 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    19681 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    19741 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    19801 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    19861 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    19921 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    19981 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    20041 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    20101 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    20161 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    20221 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    20281 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    20341 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    20401 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    20461 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    20521 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    20581 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    20641 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    20701 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    20761 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    20821 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    20881 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    20941 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    21001 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    21061 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    21121 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    21181 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    21241 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    21301 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    21361 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    21421 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    21481 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    21541 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    21601 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    21661 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    21721 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    21781 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    21841 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    21901 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    21961 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    22021 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    22081 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    22141 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    22201 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    22261 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    22321 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    22381 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    22441 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    22501 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    22561 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    22621 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    22681 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    22741 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    22801 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    22861 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    22921 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    22981 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    23041 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    23101 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    23161 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    23221 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    23281 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    23341 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    23401 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    23461 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    23521 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    23581 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    23641 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    23701 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    23761 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    23821 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    23881 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    23941 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    24001 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    24061 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    24121 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    24181 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    24241 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    24301 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    24361 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    24421 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    24481 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    24541 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    24601 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    24661 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    24721 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    24781 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    24841 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    24901 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    24961 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    25021 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    25081 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    25141 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    25201 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    25261 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    25321 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    25381 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    25441 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    25501 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    25561 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    25621 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    25681 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    25741 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    25801 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    25861 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    25921 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    25981 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    26041 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    26101 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    26161 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    26221 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    26281 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    26341 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    26401 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    26461 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    26521 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    26581 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    26641 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    26701 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    26761 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    26821 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    26881 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    26941 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    27001 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    27061 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    27121 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    27181 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    27241 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    27301 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    27361 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    27421 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    27481 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    27541 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    27601 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    27661 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    27721 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    27781 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    27841 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    27901 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    27961 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    28021 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    28081 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    28141 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    28201 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    28261 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    28321 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    28381 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    28441 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    28501 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    28561 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    28621 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    28681 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    28741 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    28801 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    28861 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    28921 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    28981 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    29041 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    29101 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    29161 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    29221 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    29281 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    29341 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    29401 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    29461 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    29521 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    29581 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    29641 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    29701 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    29761 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    29821 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    29881 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    29941 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    30001 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    30061 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    30121 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    30181 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    30241 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    30301 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    30361 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    30421 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    30481 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    30541 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    30601 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    30661 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    30721 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    30781 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    30841 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    30901 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    30961 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    31021 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    31081 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    31141 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    31201 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    31261 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    31321 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    31381 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    31441 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    31501 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    31561 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    31621 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    31681 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    31741 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    31801 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    31861 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    31921 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    31981 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    32041 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    32101 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    32161 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    32221 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    32281 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    32341 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    32401 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    32461 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    32521 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    32581 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    32641 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    32701 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    32761 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    32821 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    32881 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    32941 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    33001 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    33061 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    33121 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    33181 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    33241 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    33301 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    33361 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    33421 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    33481 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    33541 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    33601 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    33661 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    33721 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    33781 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    33841 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    33901 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    33961 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    34021 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    34081 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    34141 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    34201 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    34261 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    34321 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    34381 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    34441 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    34501 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    34561 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    34621 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    34681 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    34741 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    34801 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    34861 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    34921 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    34981 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    35041 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    35101 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    35161 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    35221 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    35281 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    35341 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    35401 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    35461 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    35521 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    35581 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    35641 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    35701 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    35761 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    35821 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    35881 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    35941 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    36001 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    36061 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    36121 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    36181 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    36241 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    36301 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    36361 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    36421 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    36481 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    36541 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    36601 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    36661 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    36721 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    36781 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    36841 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    36901 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    36961 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    37021 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    37081 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    37141 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    37201 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    37261 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    37321 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    37381 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    37441 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    37501 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    37561 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    37621 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37681 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37741 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37801 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37861 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37921 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37981 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    38041 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    38101 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38161 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38221 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38281 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38341 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38401 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38461 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38521 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38581 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38641 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38701 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38761 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38821 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38881 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38941 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    39001 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    39061 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39121 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39181 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39241 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39301 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39361 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39421 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39481 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39541 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39601 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39661 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39721 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39781 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39841 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39901 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39961 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    40021 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    40081 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    40141 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    40201 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    40261 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    40321 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    40381 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    40441 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    40501 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    40561 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    40621 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    40681 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    40741 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    40801 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    40861 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    40921 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    40981 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    41041 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    41101 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    41161 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    41221 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    41281 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    41341 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    41401 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    41461 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    41521 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    41581 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    41641 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    41701 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    41761 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    41821 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    41881 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    41941 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    42001 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    42061 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    42121 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    42181 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    42241 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    42301 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    42361 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    42421 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    42481 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    42541 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    42601 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    42661 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    42721 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    42781 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    42841 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    42901 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    42961 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    43021 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    43081 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    43141 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    43201 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    43261 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    43321 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    43381 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    43441 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    43501 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    43561 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    43621 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    43681 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    43741 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    43801 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    43861 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    43921 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    43981 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    44041 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    44101 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    44161 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    44221 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    44281 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    44341 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    44401 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    44461 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    44521 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    44581 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    44641 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    44701 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    44761 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    44821 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    44881 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    44941 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    45001 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    45061 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    45121 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    45181 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    45241 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    45301 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    45361 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    45421 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    45481 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    45541 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    45601 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    45661 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    45721 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    45781 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    45841 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    45901 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    45961 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    46021 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    46081 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    46141 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt ttttaccctc
    46201 ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat tttttcttca
    46261 atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg atcaactgcg
    46321 aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg tttcatggaa
    46381 actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt gttcaaaaca
    46441 tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa
    46501 atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc aactactgaa
    46561 aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc caacttctct
    46621 tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta ggagtctact
    46681 gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa atcgattggt
    46741 tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg tcgggcatgg
    46801 gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac tgcacgaatc
    46861 atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct agcagctagt
    46921 tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct gtccgtcaaa
    46981 atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt tgtttcgaat
    47041 aatttttcta acttgccata gatgattcga tctactcggc caccactttc tttcatgaca
    47101 tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa ccgctgtagt
    47161 tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt taaaatcgga
    47221 tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa aaccagattg
    47281 acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt aatctcgatc
    47341 tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat cccatggtta
    47401 cagacaacga ctgatttata aacataattc aagtctcgct tgattacact ggcgatcgtc
    47461 gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc aattacttct
    47521 tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt tgttcggatg
    47581 ttttctttca atggattcat gacttggata tgattcttga ttcttacaat cagtgcagcc
    47641 atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg tacgtgtgtc
    47701 tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac ccactcgatt
    47761 gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata atttctttct
    47821 acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag
    47881 ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc atgtttcctg
    47941 attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat atcatcttct
    48001 tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac caaaggaaaa
    48061 taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat aaagaagtcg
    48121 gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga tcgttctgaa
    48181 cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata actgaccagt
    48241 gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg aaattcttct
    48301 tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa
    48361 atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt ttctaatcga
    48421 gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt acggatctcc
    48481 ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg gatcgtccgc
    48541 tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg ggctgtcatg
    48601 attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa
    48661 gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa gtggaaagaa
    48721 agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa cgaaaaagac
    48781 atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg tgaaaaagct
    48841 gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg
    48901 ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag gttgtgacag
    48961 agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca tattttacaa
    49021 aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga aaaaggagct
    49081 gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc agcataatcg
    49141 gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa taccatatct
    49201 gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct tttcgatcga
    49261 ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg tggatctgtc
    49321 gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca ccgtctgctt
    49381 tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa cagcttctgg
    49441 ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc ttttggattt
    49501 ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat atctccgctt
    49561 tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc cctcctactg
    49621 acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct gtacctggac
    49681 cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg cccccatatg
    49741 aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct ccaccatgac
    49801 cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc actaacaaaa
    49861 tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat tacaaaaaac
    49921 tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata aactgttttt
    49981 ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg tgtcggcagt
    50041 ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg cgcttcctcc
    50101 aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac gatttggtaa
    50161 agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt tctgatcact
    50221 gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca tatgcatttg
    50281 tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa gcctgatatg
    50341 cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt gcttcaccct
    50401 atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag caagcttgct
    50461 gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt tttgtgcaat
    50521 gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga tgccatttgt
    50581 agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt ctcttaactc
    50641 ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag gagttagcgt
    50701 cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg catccagtgc
    50761 atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt cctctaccaa
    50821 cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga cactcactcc
    50881 ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga tatgaagtaa
    50941 ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa tcatgactcc
    51001 tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga aaacaaatac
    51061 attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg gcaattttgg
    51121 cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga tgactaaact
    51181 gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt ctcctggatg
    51241 tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta agaaaagata
    51301 acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg ctaccccata
    51361 aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat aaggcccagt
    51421 caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg aaccaatcaa
    51481 tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg
    51541 catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg ctccatcacc
    51601 tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt gttggactgc
    51661 tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa
    51721 cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca tattataaac
    51781 aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa
    51841 agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat
    51901 aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga tttttttgtt
    51961 ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt taatttaggt
    52021 ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc ttttagtgaa
    52081 aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc aggatgcgat
    52141 tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca
    52201 ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct gccgccgatt
    52261 ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata aacaggataa
    52321 tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg atgaatgact
    52381 tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc aggtccacgt
    52441 aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc
    52501 ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa
    52561 aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc cattttggca
    52621 cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt gtatcgattt
    52681 gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat tgcttcgatg
    52741 actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag gaaatgatag
    52801 atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac tttttcagca
    52861 gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc tttagacatc
    52921 gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg taatccatgt
    52981 gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg atgtccagct
    53041 tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat accgatcaat
    53101 tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat tggccgtttc
    53161 acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct
    53221 tcaactaaac caacttcacc gtttcgcaca cggtacatcg ggtttttagg atcaggtacg
    53281 attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata cacagtacta
    53341 atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta accaacactt
    53401 gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt tgcttgatgg
    53461 ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc taaagaatca
    53521 agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt
    53581 gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc atcaatgatt
    53641 tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt gtcactagaa
    53701 tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa accgactctc
    53761 gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat accgccaaat
    53821 gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag agcaatgatt
    53881 acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa acgaacacca
    53941 cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc ttctaacaca
    54001 tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc accttggaat
    54061 tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg gctgacaacg
    54121 atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc gatgatttga
    54181 tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg ttctctggcg
    54241 tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg tcaatcgtag
    54301 gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg aaattggata
    54361 acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat cgcttcctgt
    54421 acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt ccaaatctcc
    54481 tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg gatatttata
    54541 agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac gttctgctta
    54601 ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg ccaattttat
    54661 gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc gatcaaatca
    54721 tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat gcctagatct
    54781 tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt cgtgctagtc
    54841 attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc ttcttttgtt
    54901 tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt atcttcgcta
    54961 taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag tgcatctaat
    55021 tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg aacaactaat
    55081 ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc tggtgtagaa
    55141 tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc agataaacgt
    55201 ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga accgataaac
    55261 atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg catcgtgccg
    55321 tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat accaacttcg
    55381 ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat catcgttgga
    55441 tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga ttcaccataa
    55501 gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt caaagattgt
    55561 acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat ttgcaattct
    55621 gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt atctgctttc
    55681 acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc gacaacttcg
    55741 ttaacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat tgttggtgtt
    55801 tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc tgcagctact
    55861 aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa ataagtatgg
    55921 atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa gctttgtgca
    55981 ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt caaatgttct
    56041 ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc atctactttt
    56101 tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa tacaactttg
    56161 tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc tacatacttg
    56221 ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg tttagaagcc
    56281 cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag aaacgtggcg
    56341 tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac accaaatttt
    56401 gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag atccattcct
    56461 aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag gttttccaac
    56521 aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt ttttatatac
    56581 aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt cttgaccgta
    56641 tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc tgtttgcgct
    56701 tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc agataacgta
    56761 tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc aaatgttttt
    56821 gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat tgcataaccg
    56881 cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc tgcattctca
    56941 aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg tcctgctgca
    57001 atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc ttttgtcagt
    57061 aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac taaccgatac
    57121 cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa catccctaac
    57181 ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc ctcctcagca
    57241 cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag
    57301 aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc ttcatctgca
    57361 gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag aattatttat
    57421 ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag gtgtcccttt
    57481 gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat tttatccata
    57541 tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt cggtcaatga
    57601 gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta ttccgaactt
    57661 ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc aacatcattt
    57721 accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc ctggcatgac
    57781 caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac atcctcttgc
    57841 taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga tgctgtcaaa
    57901 gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta tcgaattgac
    57961 gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc aaggattagc
    58021 tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg
    58081 ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct tcacagaaga
    58141 tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa
    58201 aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag
    58261 aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca attttcgtaa
    58321 attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct attgtcctag
    58381 acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca tctagctttt
    58441 ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag gctggaaaat
    58501 acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaagaatat
    58561 tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga
    58621 ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa
    58681 aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt tcttttttcg
    58741 ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata ccacgtaaga
    58801 atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc ttttttatca
    58861 actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt aaaatgttta
    58921 ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt tcatgatgaa
    58981 attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat cagttgaaac
    59041 ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat tgatgtattc
    59101 actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt tagtagaaca
    59161 attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag ggacaaccga
    59221 acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag gctgggacat
    59281 catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa caaatgacct
    59341 ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac
    59401 aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg ggacattgaa
    59461 aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga tcgatttgac
    59521 gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt
    59581 gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc atgaatccca
    59641 ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa cagaagagat
    59701 tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt gccgattaga
    59761 ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat tgaaaaaatt
    59821 gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt ctttctgctt
    59881 tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct gcctgcatca
    59941 ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct
    60001 tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat taaaatatca
    60061 tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt tagaaacata
    60121 tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa
    60181 agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac tgatccaaca
    60241 aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc aaggagaacc
    60301 ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac gtgtctgctt
    60361 cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag atatcttgat
    60421 ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt ctattccaac
    60481 gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat agtaacggct
    60541 agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc atgatccctt
    60601 tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat aactgttgtg
    60661 tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa ataaccatcc
    60721 cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt
    60781 ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct tgagctaaga
    60841 cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc atagccaata
    60901 aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa tgataaccag
    60961 ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga tctggcgact
    61021 cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat aaaatctgta
    61081 ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc aatccttgag
    61141 ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa atccgccggc
    61201 cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc atatcagctg
    61261 cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg gatcttttca
    61321 aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca tttttctgca
    61381 aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca tcgactattg
    61441 gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct tctaaaactt
    61501 tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct ttgtgatccc
    61561 ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct ttgactgcac
    61621 tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg gttaaagtct
    61681 catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa acacgctgac
    61741 cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca gaagaaagag
    61801 ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct cctttttcct
    61861 ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt gcttctattt
    61921 ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc
    61981 cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag tttttcataa
    62041 tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt tcattttttg
    62101 tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt tctaccgaag
    62161 gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg gatagaatca
    62221 tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca atcgtagccg
    62281 aaagaagaat atgccaatcc ataccatatc gaaatgtcgt aaaaataact aagaataggc
    62341 tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt ttaatcagag
    62401 gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt tctgtcgtgt
    62461 ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt cctgaaaaac
    62521 ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga cttttttcag
    62581 atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt gcttcgatta
    62641 atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct ccttttacca
    62701 gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct tcttgtccca
    62761 tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag taactaacga
    62821 gtccattgac gatcactacg aataaaataa tacttccttc aatatattca ctagaagcaa
    62881 atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa tgctttgcta
    62941 tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct tgatcagtca
    63001 ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag tttttcaata
    63061 catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa ttgatactgt
    63121 tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt ctgaaataat
    63181 tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc ttttatatac
    63241 aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg tcgtgctttc
    63301 gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat ctttatatct
    63361 ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca agatcgaaga
    63421 ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact
    63481 atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg taattaaaat
    63541 accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca aacttttttc
    63601 ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg ctgaggacaa
    63661 agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat ggatcacttg
    63721 cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa caacaaataa
    63781 aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac agttgattgc
    63841 ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga tcatttcaat
    63901 tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa agccaaacgg
    63961 tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg tatgaaaatc
    64021 tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga tcttgatgat
    64081 tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga ctgccattaa
    64141 gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc agaaatggat
    64201 atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa gccaataagt
    64261 ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat cactgataga
    64321 gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac cataaatagc
    64381 caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa ttgcctttgc
    64441 tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact ggatatttcg
    64501 attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt tctttttcaa
    64561 gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata cagagtggta
    64621 ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat gttccagctt
    64681 tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg acagtccaaa
    64741 ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc aactttgcat
    64801 gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata cagtagataa
    64861 cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc agcaaataaa
    64921 aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg gatacgtcaa
    64981 attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta gcactttctg
    65041 tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca ccaatgaaga
    65101 caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg caataaatcg
    65161 aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca tttgctacaa
    65221 ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat actccgcgaa
    65281 tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt tcgataccag
    65341 taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta gtatctggta
    65401 ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca actttgatcc
    65461 acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct aaccaaacac
    65521 cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg atgatccaat
    65581 aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc acattatcga
    65641 taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca ggaaacattt
    65701 ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca ttcgataccc
    65761 aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag atcaatgaga
    65821 ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg
    65881 ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt ccgataaagg
    65941 tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct tctttcaata
    66001 gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac aaggtttcat
    66061 cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta gtgaactggg
    66121 tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag aatatcttgg
    66181 cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa ataattgtct
    66241 gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg gatgatttcg
    66301 tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa aattcgatca
    66361 tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg ttcttttttc
    66421 cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc accgtaatct
    66481 tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc gccatccata
    66541 taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa atctgtacag
    66601 ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa tggatagact
    66661 tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct gaatctttga
    66721 gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa tggcaataca
    66781 cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc caaaatataa
    66841 gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc gaaataagaa
    66901 atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata agggacatag
    66961 cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct gaacccttca
    67021 tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc aaagaattca
    67081 gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga acgcgcatag
    67141 ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt taacctttct
    67201 cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt tcctacgaca
    67261 gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt tttttccaaa
    67321 gcattaatat ccattcgata atcctcttga actgggatag caaccatctg atctaagccg
    67381 actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg gaccagccat
    67441 ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac ctcatccatt
    67501 tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa attcaaaagt
    67561 tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc taaaggaatc
    67621 gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc atctgctgca
    67681 atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc tacttctgct
    67741 tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt ccacagcatc
    67801 gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg tccccagtaa
    67861 cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac ttcatccaag
    67921 acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc tccttcgcta
    67981 attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc atctaccatc
    68041 ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat aaagacggcc
    68101 ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat gtcttgcaga
    68161 tacatgtgtt tatc
//
LOCUS       DAZKAO010000002        59044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000002 DAZKAO010000000
VERSION     DAZKAO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.002"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8H8_000061"
     CDS             complement(59..187)
                     /locus_tag="ACG8H8_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP8985174.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8H8_000062"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8H8_000062"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP8985175.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8H8_000063"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8H8_000063"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP8985176.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8H8_000064"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8H8_000064"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP8985177.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8H8_000065"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8H8_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP8985178.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8H8_000066"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8H8_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP8985179.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8H8_000067"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8H8_000067"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP8985180.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8H8_000068"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8H8_000068"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP8985181.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8H8_000069"
     CDS             10491..12050
                     /locus_tag="ACG8H8_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP8985182.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8H8_000070"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8H8_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP8985183.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8H8_000071"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8H8_000071"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP8985184.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8H8_000072"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8H8_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8985185.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8H8_000073"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8H8_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985186.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8H8_000074"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8H8_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985187.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8H8_000075"
     CDS             17616..17771
                     /locus_tag="ACG8H8_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP8985188.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8H8_000076"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8H8_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP8985189.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8H8_000077"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8H8_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP8985190.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8H8_000078"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8H8_000078"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP8985191.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8H8_000079"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8H8_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985192.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8H8_000080"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8H8_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP8985193.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8H8_000081"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8H8_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP8985194.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8H8_000082"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8H8_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985195.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8H8_000083"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8H8_000083"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP8985196.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8H8_000084"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8H8_000084"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP8985197.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8H8_000085"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8H8_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP8985198.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8H8_000086"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8H8_000086"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP8985199.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8H8_000087"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8H8_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP8985200.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMTIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8H8_000088"
     CDS             34320..34841
                     /locus_tag="ACG8H8_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP8985201.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8H8_000089"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8H8_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8H8_000090"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8H8_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985202.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8H8_000091"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8H8_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP8985203.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8H8_000092"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8H8_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP8985204.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8H8_000093"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8H8_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP8985205.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8H8_000094"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8H8_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP8985206.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8H8_000095"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8H8_000095"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP8985207.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8H8_000096"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8H8_000096"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP8985208.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8H8_000097"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8H8_000097"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP8985209.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8H8_000098"
     CDS             47833..49335
                     /locus_tag="ACG8H8_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP8985210.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8H8_000099"
     CDS             49337..50074
                     /locus_tag="ACG8H8_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8985211.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8H8_000100"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8H8_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP8985212.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8H8_000101"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8H8_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP8985213.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8H8_000102"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8H8_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP8985214.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8H8_000103"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8H8_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP8985215.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8H8_000104"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8H8_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP8985216.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG8H8_000105"
     CDS             complement(56756..56983)
                     /locus_tag="ACG8H8_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP8985217.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8H8_000106"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8H8_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP8985218.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..58415)
                     /locus_tag="ACG8H8_000107"
     CDS             complement(57969..58415)
                     /locus_tag="ACG8H8_000107"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP8985219.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(58396..58992)
                     /locus_tag="ACG8H8_000108"
     CDS             complement(58396..58992)
                     /locus_tag="ACG8H8_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP8985220.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
BASE COUNT        16267 a        12799 c        10273 g        19705 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagtcat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaa
//
LOCUS       DAZKAO010000003        56912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000003 DAZKAO010000000
VERSION     DAZKAO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.003"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(457..960)
                     /locus_tag="ACG8H8_000109"
     CDS             complement(457..960)
                     /locus_tag="ACG8H8_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8985221.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1319..2344
                     /locus_tag="ACG8H8_000110"
     CDS             1319..2344
                     /locus_tag="ACG8H8_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8985222.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2667..3545
                     /locus_tag="ACG8H8_000111"
     CDS             2667..3545
                     /locus_tag="ACG8H8_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP8985223.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4021..4137
                     /locus_tag="ACG8H8_000112"
     CDS             4021..4137
                     /locus_tag="ACG8H8_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP8985224.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5065..5403
                     /locus_tag="ACG8H8_000113"
     CDS             5065..5403
                     /locus_tag="ACG8H8_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985225.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5497..5727
                     /locus_tag="ACG8H8_000114"
     CDS             5497..5727
                     /locus_tag="ACG8H8_000114"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP8985226.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6127..6498
                     /locus_tag="ACG8H8_000115"
     CDS             6127..6498
                     /locus_tag="ACG8H8_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP8985227.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6772..7464)
                     /locus_tag="ACG8H8_000116"
     CDS             complement(6772..7464)
                     /locus_tag="ACG8H8_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8985228.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7467..8252)
                     /locus_tag="ACG8H8_000117"
     CDS             complement(7467..8252)
                     /locus_tag="ACG8H8_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8985229.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8489..9889
                     /gene="lacG"
                     /locus_tag="ACG8H8_000118"
     CDS             8489..9889
                     /gene="lacG"
                     /locus_tag="ACG8H8_000118"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP8985230.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9914..10231
                     /locus_tag="ACG8H8_000119"
     CDS             9914..10231
                     /locus_tag="ACG8H8_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8985231.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10244..11917
                     /locus_tag="ACG8H8_000120"
     CDS             10244..11917
                     /locus_tag="ACG8H8_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP8985232.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11931..13562
                     /locus_tag="ACG8H8_000121"
     CDS             11931..13562
                     /locus_tag="ACG8H8_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP8985233.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14053..15618)
                     /gene="guaA"
                     /locus_tag="ACG8H8_000122"
     CDS             complement(14053..15618)
                     /gene="guaA"
                     /locus_tag="ACG8H8_000122"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP8985234.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15677..16600)
                     /gene="coaA"
                     /locus_tag="ACG8H8_000123"
     CDS             complement(15677..16600)
                     /gene="coaA"
                     /locus_tag="ACG8H8_000123"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP8985235.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16796..17401
                     /locus_tag="ACG8H8_000124"
     CDS             16796..17401
                     /locus_tag="ACG8H8_000124"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8985236.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17709..18725
                     /locus_tag="ACG8H8_000125"
     CDS             17709..18725
                     /locus_tag="ACG8H8_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985237.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18866..20167
                     /locus_tag="ACG8H8_000126"
     CDS             18866..20167
                     /locus_tag="ACG8H8_000126"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP8985238.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20213..20875
                     /gene="deoC"
                     /locus_tag="ACG8H8_000127"
     CDS             20213..20875
                     /gene="deoC"
                     /locus_tag="ACG8H8_000127"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP8985239.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20896..21294
                     /locus_tag="ACG8H8_000128"
     CDS             20896..21294
                     /locus_tag="ACG8H8_000128"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP8985240.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21360..22442
                     /locus_tag="ACG8H8_000129"
     CDS             21360..22442
                     /locus_tag="ACG8H8_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8985241.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22654..24216
                     /locus_tag="ACG8H8_000130"
     CDS             22654..24216
                     /locus_tag="ACG8H8_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985242.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24209..25342
                     /locus_tag="ACG8H8_000131"
     CDS             24209..25342
                     /locus_tag="ACG8H8_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985243.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25342..26298
                     /locus_tag="ACG8H8_000132"
     CDS             25342..26298
                     /locus_tag="ACG8H8_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985244.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26397..27563
                     /gene="deoB"
                     /locus_tag="ACG8H8_000133"
     CDS             26397..27563
                     /gene="deoB"
                     /locus_tag="ACG8H8_000133"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP8985245.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27587..28405
                     /locus_tag="ACG8H8_000134"
     CDS             27587..28405
                     /locus_tag="ACG8H8_000134"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP8985246.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28422..29129
                     /gene="deoD"
                     /locus_tag="ACG8H8_000135"
     CDS             28422..29129
                     /gene="deoD"
                     /locus_tag="ACG8H8_000135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP8985247.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29427..30113)
                     /gene="gpmA"
                     /locus_tag="ACG8H8_000136"
     CDS             complement(29427..30113)
                     /gene="gpmA"
                     /locus_tag="ACG8H8_000136"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP8985248.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30285..30866)
                     /locus_tag="ACG8H8_000137"
     CDS             complement(30285..30866)
                     /locus_tag="ACG8H8_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8985249.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30999..31682)
                     /gene="rpiA"
                     /locus_tag="ACG8H8_000138"
     CDS             complement(30999..31682)
                     /gene="rpiA"
                     /locus_tag="ACG8H8_000138"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP8985250.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32001..32414
                     /gene="rpsL"
                     /locus_tag="ACG8H8_000139"
     CDS             32001..32414
                     /gene="rpsL"
                     /locus_tag="ACG8H8_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP8985251.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32498..32968
                     /gene="rpsG"
                     /locus_tag="ACG8H8_000140"
     CDS             32498..32968
                     /gene="rpsG"
                     /locus_tag="ACG8H8_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP8985252.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33062..35149
                     /gene="fusA"
                     /locus_tag="ACG8H8_000141"
     CDS             33062..35149
                     /gene="fusA"
                     /locus_tag="ACG8H8_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP8985253.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35321..36508
                     /gene="tuf"
                     /locus_tag="ACG8H8_000142"
     CDS             35321..36508
                     /gene="tuf"
                     /locus_tag="ACG8H8_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP8985254.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36915..37223
                     /gene="rpsJ"
                     /locus_tag="ACG8H8_000143"
     CDS             36915..37223
                     /gene="rpsJ"
                     /locus_tag="ACG8H8_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP8985255.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37254..37883
                     /gene="rplC"
                     /locus_tag="ACG8H8_000144"
     CDS             37254..37883
                     /gene="rplC"
                     /locus_tag="ACG8H8_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP8985256.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37911..38534
                     /gene="rplD"
                     /locus_tag="ACG8H8_000145"
     CDS             37911..38534
                     /gene="rplD"
                     /locus_tag="ACG8H8_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP8985257.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38534..38824
                     /gene="rplW"
                     /locus_tag="ACG8H8_000146"
     CDS             38534..38824
                     /gene="rplW"
                     /locus_tag="ACG8H8_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP8985258.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38863..39696
                     /gene="rplB"
                     /locus_tag="ACG8H8_000147"
     CDS             38863..39696
                     /gene="rplB"
                     /locus_tag="ACG8H8_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP8985259.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39739..40017
                     /gene="rpsS"
                     /locus_tag="ACG8H8_000148"
     CDS             39739..40017
                     /gene="rpsS"
                     /locus_tag="ACG8H8_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP8985260.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40040..40387
                     /gene="rplV"
                     /locus_tag="ACG8H8_000149"
     CDS             40040..40387
                     /gene="rplV"
                     /locus_tag="ACG8H8_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP8985261.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40401..41057
                     /gene="rpsC"
                     /locus_tag="ACG8H8_000150"
     CDS             40401..41057
                     /gene="rpsC"
                     /locus_tag="ACG8H8_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP8985262.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41060..41494
                     /gene="rplP"
                     /locus_tag="ACG8H8_000151"
     CDS             41060..41494
                     /gene="rplP"
                     /locus_tag="ACG8H8_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP8985263.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41484..41672
                     /gene="rpmC"
                     /locus_tag="ACG8H8_000152"
     CDS             41484..41672
                     /gene="rpmC"
                     /locus_tag="ACG8H8_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP8985264.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41697..41963
                     /gene="rpsQ"
                     /locus_tag="ACG8H8_000153"
     CDS             41697..41963
                     /gene="rpsQ"
                     /locus_tag="ACG8H8_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP8985265.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42021..42389
                     /gene="rplN"
                     /locus_tag="ACG8H8_000154"
     CDS             42021..42389
                     /gene="rplN"
                     /locus_tag="ACG8H8_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP8985266.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42426..42734
                     /gene="rplX"
                     /locus_tag="ACG8H8_000155"
     CDS             42426..42734
                     /gene="rplX"
                     /locus_tag="ACG8H8_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP8985267.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42762..43301
                     /gene="rplE"
                     /locus_tag="ACG8H8_000156"
     CDS             42762..43301
                     /gene="rplE"
                     /locus_tag="ACG8H8_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP8985268.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43320..43505
                     /locus_tag="ACG8H8_000157"
     CDS             43320..43505
                     /locus_tag="ACG8H8_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP8985269.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43541..43939
                     /gene="rpsH"
                     /locus_tag="ACG8H8_000158"
     CDS             43541..43939
                     /gene="rpsH"
                     /locus_tag="ACG8H8_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP8985270.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43971..44507
                     /gene="rplF"
                     /locus_tag="ACG8H8_000159"
     CDS             43971..44507
                     /gene="rplF"
                     /locus_tag="ACG8H8_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP8985271.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44668..45024
                     /gene="rplR"
                     /locus_tag="ACG8H8_000160"
     CDS             44668..45024
                     /gene="rplR"
                     /locus_tag="ACG8H8_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP8985272.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45045..45545
                     /gene="rpsE"
                     /locus_tag="ACG8H8_000161"
     CDS             45045..45545
                     /gene="rpsE"
                     /locus_tag="ACG8H8_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP8985273.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45560..45739
                     /gene="rpmD"
                     /locus_tag="ACG8H8_000162"
     CDS             45560..45739
                     /gene="rpmD"
                     /locus_tag="ACG8H8_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP8985274.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45783..46223
                     /gene="rplO"
                     /locus_tag="ACG8H8_000163"
     CDS             45783..46223
                     /gene="rplO"
                     /locus_tag="ACG8H8_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP8985275.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46223..47518
                     /gene="secY"
                     /locus_tag="ACG8H8_000164"
     CDS             46223..47518
                     /gene="secY"
                     /locus_tag="ACG8H8_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP8985276.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47580..48227
                     /locus_tag="ACG8H8_000165"
     CDS             47580..48227
                     /locus_tag="ACG8H8_000165"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP8985277.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48418..48636
                     /gene="infA"
                     /locus_tag="ACG8H8_000166"
     CDS             48418..48636
                     /gene="infA"
                     /locus_tag="ACG8H8_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP8985278.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48669..48785
                     /gene="rpmJ"
                     /locus_tag="ACG8H8_000167"
     CDS             48669..48785
                     /gene="rpmJ"
                     /locus_tag="ACG8H8_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP8985279.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48803..49168
                     /gene="rpsM"
                     /locus_tag="ACG8H8_000168"
     CDS             48803..49168
                     /gene="rpsM"
                     /locus_tag="ACG8H8_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP8985280.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49196..49585
                     /gene="rpsK"
                     /locus_tag="ACG8H8_000169"
     CDS             49196..49585
                     /gene="rpsK"
                     /locus_tag="ACG8H8_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP8985281.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49665..50603
                     /locus_tag="ACG8H8_000170"
     CDS             49665..50603
                     /locus_tag="ACG8H8_000170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP8985282.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50631..51011
                     /gene="rplQ"
                     /locus_tag="ACG8H8_000171"
     CDS             50631..51011
                     /gene="rplQ"
                     /locus_tag="ACG8H8_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP8985283.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51283..52122
                     /locus_tag="ACG8H8_000172"
     CDS             51283..52122
                     /locus_tag="ACG8H8_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8985284.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52098..52967
                     /locus_tag="ACG8H8_000173"
     CDS             52098..52967
                     /locus_tag="ACG8H8_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8985285.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52957..53754
                     /locus_tag="ACG8H8_000174"
     CDS             52957..53754
                     /locus_tag="ACG8H8_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP8985286.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53957..54703
                     /gene="truA"
                     /locus_tag="ACG8H8_000175"
     CDS             53957..54703
                     /gene="truA"
                     /locus_tag="ACG8H8_000175"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP8985287.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55367..55561
                     /locus_tag="ACG8H8_000176"
                     /pseudo
     CDS             55367..55561
                     /locus_tag="ACG8H8_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55749..56525)
                     /locus_tag="ACG8H8_000177"
     CDS             complement(55749..56525)
                     /locus_tag="ACG8H8_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8985288.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56708..>56912
                     /locus_tag="ACG8H8_000178"
     CDS             56708..>56912
                     /locus_tag="ACG8H8_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFP8985289.1"
                     /translation="MKELGEKVHLTGPAVSARVTKLEESGVIENYTVKLNMEKLGFSI
                     HAFLIIITQNLNHKPYLSFIKNHE"
BASE COUNT        18186 a        10163 c        12143 g        16420 t
ORIGIN      
        1 tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa
       61 aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga
      121 aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata
      181 tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga
      241 agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga
      301 atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag
      361 atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag atacacattg
      421 gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt
      481 caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag
      541 acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat
      601 cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag
      661 gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa
      721 agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt
      781 ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag
      841 attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa
      901 aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat
      961 agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt
     1021 atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt tttgttggga
     1081 atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt
     1141 ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt
     1201 tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg
     1261 ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt
     1321 gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct
     1381 gatgctactt atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc
     1441 aagtttttca tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata
     1501 tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat
     1561 cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag
     1621 cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct
     1681 ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta
     1741 tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc
     1801 acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt
     1861 agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca
     1921 cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc
     1981 agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg
     2041 gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct
     2101 gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa
     2161 tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat
     2221 ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt
     2281 tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga
     2341 atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta
     2401 attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga
     2461 aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa
     2521 ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc
     2581 ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg
     2641 tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga
     2701 tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg
     2761 ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata
     2821 gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct
     2881 atgaagagga accttttgca gcattagcta tttatctata tgcagcttct caaagttgga
     2941 aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt
     3001 ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt
     3061 tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa
     3121 tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc
     3181 ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg
     3241 ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata
     3301 cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt
     3361 ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga
     3421 tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac
     3481 aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt
     3541 cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt
     3601 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
     3661 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt
     3721 aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag
     3781 gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag
     3841 ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg
     3901 actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta
     3961 attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat
     4021 atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca
     4081 cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa
     4141 gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg
     4201 tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac atatgataaa
     4261 ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact
     4321 acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc
     4381 tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg
     4441 tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa
     4501 atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt
     4561 ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt
     4621 cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa
     4681 gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc
     4741 aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag
     4801 atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta
     4861 ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt
     4921 cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat
     4981 aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct
     5041 ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac
     5101 ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca
     5161 ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac
     5221 gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca
     5281 ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact
     5341 gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg
     5401 tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac
     5461 aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa
     5521 aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc
     5581 aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata
     5641 gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt
     5701 ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata
     5761 ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa tgcagtataa
     5821 agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct acacacttct
     5881 atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat
     5941 tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga
     6001 acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa
     6061 aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat
     6121 aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca
     6181 ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt
     6241 agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc
     6301 atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact
     6361 tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt
     6421 tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa
     6481 ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact
     6541 cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat
     6601 tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc
     6661 tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat
     6721 aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt
     6781 aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc
     6841 tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat
     6901 aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac
     6961 aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg
     7021 cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc
     7081 taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt
     7141 taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg
     7201 aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa
     7261 tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt
     7321 cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc
     7381 atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt
     7441 gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca
     7501 gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga
     7561 tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg
     7621 aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt
     7681 gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg
     7741 ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat
     7801 gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga
     7861 gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa
     7921 tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga
     7981 atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt
     8041 tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca
     8101 cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa
     8161 acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct
     8221 acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc
     8281 tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt
     8341 tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt
     8401 gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa
     8461 ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg
     8521 cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt
     8581 tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt
     8641 ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag
     8701 attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg
     8761 aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt
     8821 gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg
     8881 agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt
     8941 caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg
     9001 tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat
     9061 gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat
     9121 tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca
     9181 tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg
     9241 ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag
     9301 ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta
     9361 cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat
     9421 ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga
     9481 acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg
     9541 tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat
     9601 cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc
     9661 gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt
     9721 aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta
     9781 taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga
     9841 atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat
     9901 ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata
     9961 ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt
    10021 tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc
    10081 gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt
    10141 tatccatggg caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt
    10201 tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa
    10261 acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc
    10321 cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt
    10381 gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct
    10441 atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc
    10501 tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac
    10561 ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg
    10621 tggattttca acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt
    10681 tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa
    10741 agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt
    10801 atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga
    10861 agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc
    10921 tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt
    10981 ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa
    11041 ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat
    11101 gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa
    11161 gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc
    11221 attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac
    11281 acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt
    11341 tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat
    11401 gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct
    11461 accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt
    11521 gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag
    11581 tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg
    11641 gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat
    11701 cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt
    11761 ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt
    11821 aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga
    11881 aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg
    11941 gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt
    12001 caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt
    12061 cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag
    12121 agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata
    12181 atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg
    12241 caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt
    12301 gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa
    12361 tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg
    12421 tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg
    12481 gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg
    12541 gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg
    12601 atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt
    12661 catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc
    12721 aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag
    12781 cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag
    12841 cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg
    12901 ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc
    12961 tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc
    13021 aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct
    13081 tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa
    13141 gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag
    13201 gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa
    13261 ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca
    13321 aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt
    13381 tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat
    13441 ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt
    13501 atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat
    13561 agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag
    13621 aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt
    13681 catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga
    13741 gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata
    13801 aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag
    13861 atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa
    13921 gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa
    13981 tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat
    14041 tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
    14101 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
    14161 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
    14221 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
    14281 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
    14341 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
    14401 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
    14461 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
    14521 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
    14581 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
    14641 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
    14701 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
    14761 tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
    14821 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
    14881 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
    14941 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
    15001 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
    15061 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
    15121 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
    15181 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
    15241 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
    15301 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
    15361 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
    15421 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
    15481 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
    15541 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
    15601 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
    15661 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
    15721 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
    15781 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
    15841 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    15901 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    15961 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    16021 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    16081 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    16141 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    16201 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    16261 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    16321 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    16381 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    16441 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    16501 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    16561 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    16621 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    16681 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    16741 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg gagacatgac
    16801 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    16861 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    16921 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    16981 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    17041 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    17101 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    17161 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    17221 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    17281 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    17341 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    17401 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    17461 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    17521 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    17581 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    17641 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    17701 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    17761 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    17821 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    17881 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    17941 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    18001 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    18061 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    18121 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    18181 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    18241 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    18301 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    18361 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    18421 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    18481 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    18541 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    18601 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    18661 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    18721 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    18781 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    18841 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    18901 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    18961 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    19021 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    19081 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    19141 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    19201 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    19261 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    19321 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    19381 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    19441 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    19501 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    19561 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    19621 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    19681 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    19741 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    19801 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    19861 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    19921 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    19981 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    20041 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    20101 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    20161 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    20221 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    20281 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    20341 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    20401 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    20461 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    20521 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    20581 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    20641 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    20701 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    20761 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    20821 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    20881 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    20941 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    21001 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    21061 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    21121 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    21181 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    21241 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    21301 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    21361 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    21421 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    21481 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    21541 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    21601 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    21661 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    21721 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    21781 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    21841 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    21901 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    21961 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    22021 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    22081 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    22141 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    22201 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    22261 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    22321 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    22381 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    22441 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    22501 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    22561 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    22621 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    22681 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    22741 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    22801 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    22861 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    22921 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    22981 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    23041 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    23101 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    23161 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    23221 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    23281 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    23341 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    23401 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    23461 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    23521 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    23581 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    23641 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    23701 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    23761 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    23821 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    23881 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    23941 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    24001 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    24061 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    24121 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    24181 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    24241 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    24301 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    24361 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    24421 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    24481 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    24541 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    24601 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    24661 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    24721 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    24781 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    24841 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    24901 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    24961 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    25021 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    25081 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    25141 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    25201 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    25261 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    25321 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    25381 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    25441 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    25501 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    25561 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    25621 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    25681 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    25741 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    25801 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    25861 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    25921 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    25981 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    26041 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    26101 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    26161 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    26221 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    26281 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    26341 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    26401 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    26461 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    26521 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    26581 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    26641 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    26701 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    26761 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    26821 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    26881 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    26941 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    27001 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    27061 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    27121 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    27181 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    27241 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    27301 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    27361 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    27421 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    27481 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    27541 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    27601 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    27661 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    27721 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    27781 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    27841 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    27901 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    27961 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    28021 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    28081 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    28141 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    28201 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    28261 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    28321 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    28381 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    28441 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    28501 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    28561 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    28621 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    28681 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    28741 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    28801 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    28861 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    28921 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    28981 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    29041 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    29101 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    29161 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    29221 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    29281 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    29341 gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg
    29401 tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca
    29461 tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg
    29521 tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact
    29581 aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg
    29641 cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg
    29701 taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta
    29761 tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt
    29821 ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt
    29881 ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt
    29941 aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct
    30001 tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg
    30061 ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc
    30121 tctcctttta agataggttt atatttctga aattttcagt tctgttttat tgtacaattt
    30181 tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat
    30241 ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc
    30301 tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat
    30361 tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg
    30421 atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct
    30481 aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt
    30541 aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag
    30601 tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct
    30661 aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac
    30721 tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct
    30781 tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt
    30841 gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg
    30901 atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc
    30961 cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca
    31021 agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc
    31081 ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg
    31141 atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg
    31201 ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt
    31261 tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat
    31321 ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa
    31381 agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg
    31441 tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt
    31501 tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca
    31561 cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct
    31621 acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc
    31681 atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga
    31741 tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc
    31801 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    31861 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    31921 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    31981 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    32041 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    32101 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    32161 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    32221 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    32281 tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg
    32341 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    32401 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    32461 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    32521 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    32581 ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga
    32641 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    32701 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    32761 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    32821 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    32881 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    32941 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    33001 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    33061 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    33121 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    33181 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    33241 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    33301 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    33361 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    33421 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    33481 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    33541 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    33601 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    33661 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    33721 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    33781 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    33841 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    33901 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    33961 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    34021 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    34081 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    34141 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    34201 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    34261 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    34321 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    34381 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    34441 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    34501 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    34561 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    34621 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    34681 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    34741 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    34801 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    34861 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    34921 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    34981 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    35041 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    35101 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac
    35161 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    35221 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    35281 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    35341 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    35401 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    35461 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    35521 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    35581 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    35641 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    35701 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    35761 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    35821 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    35881 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    35941 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    36001 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    36061 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    36121 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    36181 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    36241 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    36301 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    36361 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    36421 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    36481 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    36541 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    36601 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
    36661 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
    36721 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
    36781 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
    36841 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
    36901 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
    36961 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
    37021 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
    37081 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
    37141 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
    37201 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
    37261 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
    37321 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
    37381 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
    37441 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
    37501 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
    37561 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
    37621 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
    37681 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
    37741 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
    37801 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
    37861 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
    37921 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
    37981 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
    38041 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
    38101 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
    38161 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
    38221 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
    38281 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
    38341 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
    38401 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
    38461 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
    38521 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
    38581 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
    38641 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
    38701 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
    38761 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
    38821 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
    38881 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
    38941 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
    39001 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
    39061 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
    39121 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
    39181 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
    39241 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
    39301 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
    39361 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
    39421 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
    39481 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
    39541 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
    39601 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
    39661 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
    39721 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
    39781 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
    39841 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
    39901 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
    39961 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
    40021 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
    40081 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
    40141 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
    40201 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
    40261 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
    40321 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
    40381 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
    40441 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
    40501 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
    40561 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
    40621 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
    40681 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
    40741 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
    40801 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
    40861 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
    40921 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
    40981 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
    41041 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
    41101 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
    41161 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
    41221 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
    41281 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
    41341 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
    41401 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
    41461 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
    41521 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
    41581 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
    41641 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
    41701 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
    41761 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
    41821 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
    41881 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
    41941 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
    42001 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
    42061 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
    42121 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
    42181 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
    42241 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
    42301 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
    42361 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
    42421 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
    42481 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
    42541 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
    42601 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
    42661 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
    42721 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
    42781 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
    42841 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
    42901 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
    42961 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
    43021 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
    43081 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
    43141 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
    43201 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
    43261 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
    43321 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
    43381 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
    43441 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
    43501 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
    43561 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
    43621 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
    43681 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
    43741 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
    43801 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
    43861 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
    43921 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
    43981 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
    44041 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
    44101 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
    44161 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
    44221 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
    44281 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
    44341 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
    44401 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
    44461 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
    44521 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
    44581 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
    44641 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
    44701 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
    44761 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
    44821 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
    44881 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
    44941 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
    45001 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
    45061 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
    45121 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
    45181 tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
    45241 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
    45301 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
    45361 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
    45421 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
    45481 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
    45541 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
    45601 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
    45661 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
    45721 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
    45781 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
    45841 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
    45901 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
    45961 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
    46021 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
    46081 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
    46141 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
    46201 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
    46261 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
    46321 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
    46381 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
    46441 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
    46501 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
    46561 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    46621 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    46681 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    46741 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    46801 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    46861 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    46921 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    46981 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    47041 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    47101 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    47161 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    47221 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    47281 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    47341 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    47401 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    47461 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    47521 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    47581 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    47641 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    47701 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    47761 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    47821 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    47881 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    47941 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    48001 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    48061 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    48121 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    48181 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    48241 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    48301 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    48361 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    48421 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    48481 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    48541 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    48601 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    48661 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    48721 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    48781 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    48841 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    48901 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    48961 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    49021 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    49081 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    49141 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    49201 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    49261 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    49321 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    49381 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    49441 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    49501 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    49561 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    49621 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    49681 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    49741 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    49801 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    49861 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    49921 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    49981 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    50041 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    50101 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    50161 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    50221 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    50281 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    50341 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    50401 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    50461 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    50521 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    50581 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    50641 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    50701 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    50761 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    50821 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    50881 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    50941 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    51001 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    51061 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    51121 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    51181 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    51241 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    51301 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    51361 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    51421 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    51481 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt
    51541 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    51601 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    51661 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    51721 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    51781 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    51841 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    51901 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    51961 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    52021 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    52081 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    52141 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    52201 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    52261 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    52321 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    52381 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    52441 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    52501 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    52561 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    52621 gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    52681 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    52741 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    52801 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    52861 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    52921 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    52981 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    53041 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    53101 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    53161 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    53221 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    53281 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    53341 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    53401 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    53461 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    53521 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    53581 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    53641 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    53701 gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg ataaacgttc
    53761 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcgagcagtg
    53821 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    53881 gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac cttttaggaa
    53941 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg gaacaaattt
    54001 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    54061 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    54121 agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    54181 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    54241 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    54301 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    54361 tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    54421 ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct acgaggctaa
    54481 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    54541 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    54601 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    54661 acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg tgacagaagt
    54721 ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat tttgcaaaat
    54781 ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag
    54841 ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata tttttcgttt
    54901 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    54961 atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    55021 cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg
    55081 agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt ctcatctttg
    55141 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    55201 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga ggacagtcgg
    55261 aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt tgtcatttag
    55321 cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg aagcagaata
    55381 tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga ttgaacgcgt
    55441 ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag acttggaaaa
    55501 caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt tgtccacttg
    55561 gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa gcaccagtct
    55621 acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta cttaaactaa
    55681 gtactttatt ttactcttta ggattgatac agtagcatct atccctttat taataatctt
    55741 ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt ttcggaaatt
    55801 cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg gtttacagct
    55861 tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc atataaatcg
    55921 tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc atttgctact
    55981 atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca aactgtatct
    56041 cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca aacattacct
    56101 acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa ttgtatagat
    56161 tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag tcgaatttca
    56221 ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac ctctttcgct
    56281 atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat accgttgata
    56341 atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt tcttggtgat
    56401 ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa cattggatca
    56461 attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat gtgatgtata
    56521 ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat tcaattccat
    56581 acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt atgaaaggtt
    56641 gtttaccatg ttagaccaaa cagatataaa tatcattgag taactatcaa aaaatagtcg
    56701 aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg tctcggcgag
    56761 agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat taaatatgga
    56821 aaagctaggg ttttcgattc acgctttttt aattataatt acgcaaaatt taaatcataa
    56881 accctattta tcatttatta aaaatcatga aa
//
LOCUS       DAZKAO010000004        48652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000004 DAZKAO010000000
VERSION     DAZKAO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.004"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1361
                     /locus_tag="ACG8H8_000179"
     CDS             69..1361
                     /locus_tag="ACG8H8_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFP8985290.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(1455..2717)
                     /locus_tag="ACG8H8_000180"
     CDS             complement(1455..2717)
                     /locus_tag="ACG8H8_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985291.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(2710..3609)
                     /locus_tag="ACG8H8_000181"
     CDS             complement(2710..3609)
                     /locus_tag="ACG8H8_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985292.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(3754..5532)
                     /gene="uvrC"
                     /locus_tag="ACG8H8_000182"
     CDS             complement(3754..5532)
                     /gene="uvrC"
                     /locus_tag="ACG8H8_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP8985293.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(5714..6028)
                     /gene="trxA"
                     /locus_tag="ACG8H8_000183"
     CDS             complement(5714..6028)
                     /gene="trxA"
                     /locus_tag="ACG8H8_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP8985294.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6115..8475)
                     /locus_tag="ACG8H8_000184"
     CDS             complement(6115..8475)
                     /locus_tag="ACG8H8_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP8985295.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(8590..9137)
                     /locus_tag="ACG8H8_000185"
                     /pseudo
     CDS             complement(8590..9137)
                     /locus_tag="ACG8H8_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(9195..9632)
                     /gene="zapA"
                     /locus_tag="ACG8H8_000186"
     CDS             complement(9195..9632)
                     /gene="zapA"
                     /locus_tag="ACG8H8_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP8985296.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            9834..10751
                     /gene="rnhC"
                     /locus_tag="ACG8H8_000187"
     CDS             9834..10751
                     /gene="rnhC"
                     /locus_tag="ACG8H8_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP8985297.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(11007..11918)
                     /locus_tag="ACG8H8_000188"
     CDS             complement(11007..11918)
                     /locus_tag="ACG8H8_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP8985298.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12321..12899)
                     /locus_tag="ACG8H8_000189"
     CDS             complement(12321..12899)
                     /locus_tag="ACG8H8_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8985299.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(12919..13587)
                     /locus_tag="ACG8H8_000190"
     CDS             complement(12919..13587)
                     /locus_tag="ACG8H8_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985300.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(13602..14672)
                     /locus_tag="ACG8H8_000191"
     CDS             complement(13602..14672)
                     /locus_tag="ACG8H8_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985301.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(14843..16207)
                     /locus_tag="ACG8H8_000192"
     CDS             complement(14843..16207)
                     /locus_tag="ACG8H8_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP8985302.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(16204..17763)
                     /locus_tag="ACG8H8_000193"
     CDS             complement(16204..17763)
                     /locus_tag="ACG8H8_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985303.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(17819..20299)
                     /locus_tag="ACG8H8_000194"
                     /pseudo
     CDS             complement(17819..20299)
                     /locus_tag="ACG8H8_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20283..20618)
                     /locus_tag="ACG8H8_000195"
     CDS             complement(20283..20618)
                     /locus_tag="ACG8H8_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985304.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(20632..21666)
                     /locus_tag="ACG8H8_000196"
     CDS             complement(20632..21666)
                     /locus_tag="ACG8H8_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP8985305.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(21756..22436)
                     /locus_tag="ACG8H8_000197"
     CDS             complement(21756..22436)
                     /locus_tag="ACG8H8_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8985306.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(22720..23823)
                     /locus_tag="ACG8H8_000198"
     CDS             complement(22720..23823)
                     /locus_tag="ACG8H8_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP8985307.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(24452..24922)
                     /locus_tag="ACG8H8_000199"
     CDS             complement(24452..24922)
                     /locus_tag="ACG8H8_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP8985308.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            25252..26892
                     /locus_tag="ACG8H8_000200"
     CDS             25252..26892
                     /locus_tag="ACG8H8_000200"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP8985309.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            27046..28368
                     /locus_tag="ACG8H8_000201"
     CDS             27046..28368
                     /locus_tag="ACG8H8_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP8985310.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(28436..28870)
                     /locus_tag="ACG8H8_000202"
     CDS             complement(28436..28870)
                     /locus_tag="ACG8H8_000202"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985311.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(28935..29771)
                     /locus_tag="ACG8H8_000203"
     CDS             complement(28935..29771)
                     /locus_tag="ACG8H8_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP8985312.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(30513..31139)
                     /locus_tag="ACG8H8_000204"
     CDS             complement(30513..31139)
                     /locus_tag="ACG8H8_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985313.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(31434..31769)
                     /locus_tag="ACG8H8_000205"
     CDS             complement(31434..31769)
                     /locus_tag="ACG8H8_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP8985314.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(31756..32040)
                     /locus_tag="ACG8H8_000206"
     CDS             complement(31756..32040)
                     /locus_tag="ACG8H8_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP8985315.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(32096..33619)
                     /locus_tag="ACG8H8_000207"
     CDS             complement(32096..33619)
                     /locus_tag="ACG8H8_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP8985316.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(33612..34787)
                     /locus_tag="ACG8H8_000208"
     CDS             complement(33612..34787)
                     /locus_tag="ACG8H8_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8985317.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(34791..34976)
                     /locus_tag="ACG8H8_000209"
     CDS             complement(34791..34976)
                     /locus_tag="ACG8H8_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985318.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(34942..36636)
                     /locus_tag="ACG8H8_000210"
     CDS             complement(34942..36636)
                     /locus_tag="ACG8H8_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP8985319.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(36633..37106)
                     /locus_tag="ACG8H8_000211"
     CDS             complement(36633..37106)
                     /locus_tag="ACG8H8_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP8985320.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(37175..37330)
                     /locus_tag="ACG8H8_000212"
     CDS             complement(37175..37330)
                     /locus_tag="ACG8H8_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFP8985321.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(37464..37844)
                     /locus_tag="ACG8H8_000213"
     CDS             complement(37464..37844)
                     /locus_tag="ACG8H8_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP8985322.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(37848..38063)
                     /locus_tag="ACG8H8_000214"
     CDS             complement(37848..38063)
                     /locus_tag="ACG8H8_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985323.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(38066..38473)
                     /locus_tag="ACG8H8_000215"
     CDS             complement(38066..38473)
                     /locus_tag="ACG8H8_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFP8985324.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(38739..40214)
                     /locus_tag="ACG8H8_000216"
     CDS             complement(38739..40214)
                     /locus_tag="ACG8H8_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP8985325.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(40204..41052)
                     /locus_tag="ACG8H8_000217"
     CDS             complement(40204..41052)
                     /locus_tag="ACG8H8_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFP8985326.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(41089..41451)
                     /locus_tag="ACG8H8_000218"
     CDS             complement(41089..41451)
                     /locus_tag="ACG8H8_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985327.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(41452..41610)
                     /locus_tag="ACG8H8_000219"
     CDS             complement(41452..41610)
                     /locus_tag="ACG8H8_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985328.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(41751..42375)
                     /locus_tag="ACG8H8_000220"
                     /pseudo
     CDS             complement(41751..42375)
                     /locus_tag="ACG8H8_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(42372..42683)
                     /locus_tag="ACG8H8_000221"
     CDS             complement(42372..42683)
                     /locus_tag="ACG8H8_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985329.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(42727..43020)
                     /locus_tag="ACG8H8_000222"
     CDS             complement(42727..43020)
                     /locus_tag="ACG8H8_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985330.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            43214..43861
                     /locus_tag="ACG8H8_000223"
     CDS             43214..43861
                     /locus_tag="ACG8H8_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8985331.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            43922..45067
                     /locus_tag="ACG8H8_000224"
     CDS             43922..45067
                     /locus_tag="ACG8H8_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8985332.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(45506..46324)
                     /locus_tag="ACG8H8_000225"
     CDS             complement(45506..46324)
                     /locus_tag="ACG8H8_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985333.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(46424..47749)
                     /locus_tag="ACG8H8_000226"
     CDS             complement(46424..47749)
                     /locus_tag="ACG8H8_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985334.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(47918..>48652)
                     /locus_tag="ACG8H8_000227"
     CDS             complement(47918..>48652)
                     /locus_tag="ACG8H8_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985335.1"
                     /translation="HFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSAKTLTESI
                     EVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIHQQRVYFD
                     LDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVITEIYFNYL
                     KEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRKDNSISMY
                     ADIKREVKKILKVEEQYYKEIEKRNQ"
BASE COUNT        14152 a         9973 c         8048 g        16479 t
ORIGIN      
        1 atatagaaaa gacaccttga aatcctttag aattaatttg gcgaaccaac ccaaaaggag
       61 gaatcaagat gtcttacact caccttatca aagaaacatt agatattcta gacttatcag
      121 ttacttttaa cgaaaattgt ctcacaaaag aaaaatacaa agggcaaatc tgtcatattt
      181 atcgtggaaa cttgatttat acagcccaag aatgcatcca ctgtaaacac cagattgctt
      241 cagatattgt gcgttggggt acaacaaccg tccggttgtt aatgaacgat gtttctgaat
      301 gccgtaccta cctcgaattg aaaaagcaac gcttcaaatg caaagcttgt cagcggacct
      361 ttgtcgcaga tacttctgtt gcgaaaaaac actgtttcat cagtgagaaa gtccgctggt
      421 ctgtcgtgac tcgtctgaaa aaaaatacct caatgacaga gatcgccgct cagaaaaatc
      481 tatcggtctc ttctgtttac cgtattatga agcgatttta tcgtccgttg aatccttttc
      541 ggacaccgtt acctaaggta ctttgttttg atgaattcaa atcagttcgg ggtgtttcag
      601 gtgctatgag tttcatcatg atggatggac aaacacagcg cttgttggac attgtcgaga
      661 accgacaact gcctttttta aaacgttatt ttagtcattt ttcacgagaa attcgtgaag
      721 cggtcgaatg gatcgtgatt gatatgtacg ctccttatgt atcgctcgtt aaaaagctgt
      781 ttccaaaagc acagctgatc attgaccggt ttcacatcgt ccaacatatt ggtcgtacct
      841 ttcgcaatca tcgaatcaaa gaaacgaacc aactattaaa aagcaaagaa caaaaacact
      901 atcaattagg taagcaatta aaaagatact ggaaacttct tcaaaaagat gaacgcaagc
      961 ttgattatac tcgacgcctt tggcgacctg gcttcaaagc tcatttaaca gagaccgata
     1021 ttgtggatcg cttactgaaa gggagccctg ctttgcgtgt aggctatcag ttatatcaag
     1081 actttcttta cgctgtaaag gaacgagact acgtatcgtt tgaagaacta cttaccaata
     1141 atatcatgct tcccgaaggc tatcagacga ccttgagaac gttccagaaa tttctgccac
     1201 aaatcaaaaa tgctttacaa caaagttatt caaatggccc acttgaatgt ctgaacaacc
     1261 atatcaaagt gttaaaacga aatgcatatg gctttcgtag cttttataat ttcaaattgc
     1321 gcatcatgat tcgtcatggc aacgcattga ttttcaacta aaaaagatca agaggaactg
     1381 atccacttga tctccaatta ttctaagatt tctctttacc aacatcattt gacaaagagc
     1441 catttttcta tattttatgc tttttttcgt tcatttctca taattgaaat cgattgtttc
     1501 aatgaactcc ataatccgcc ttcgctatac acgagtacat tcgatttgta catgtttgca
     1561 gacaagatca tcaataagaa tgttacggct acaagtacca acaaggaaat caatgctcca
     1621 cccataccga ctgtctcatt tgctaagcga accggcataa tatatgaaga taagaatgga
     1681 atatacgaag tgatccgaat aacgatattg tttggatctg aagctcctag tatcaatcca
     1741 agcatatatc cgcctagaga tagataagtc actggcaaaa ttgcttttgc tgtatcttct
     1801 gctttgttga ccaaagagcc acataaagct gctaaaacag aaaaaatcaa aatgccaaag
     1861 atcatgaata gaagagaata ccacaagaat ggtccaatca agttatccaa tgagataccg
     1921 tctaatatat ttttgaccat ttccatatct ttgaattgac tgtatcctat accaaaaata
     1981 ataccgtata atgccatttg cgttaatgca acaagtaaaa cacctgttag ttttccgtaa
     2041 aaatgctttt gtgcagtcgt actggacagg atcacttcca tgatccgcgt gcctttttca
     2101 gaagcgatct cttgtgcaat gatctgcgca tacgtcaaaa taataatgaa caagacaatc
     2161 gttgctacat aacttacgac atattggact gtcgagttat cttctccaac tgtcattttt
     2221 ccttttgaat caaaattgac tttttgtcga gtcaaacttg cttgttggct caagctagct
     2281 acttcttctg gtgaaatgcc taattgactt gctcgaagca tcgattgcaa gccagtcagt
     2341 tgttgttgta tcagtagttg tgtactttgt cctaatgaat tttcgctata aagcatccca
     2401 tttatcttat cattctcagt tgagactact aaatacgcat cgattttttc ttccgaaagc
     2461 tctttttgtg ctttttcttc agaatcaact gctttgaact caaaatcatc tgttttgact
     2521 tggctcattt gctgactgat ttgctcatta tcagccaata cgccgatttt attgacatca
     2581 ctattttgtt gagcaaagtt tcctgctaca taaatgatac ccagcatgat aaaagggact
     2641 aatatcatga tcaagaaaga aatcgactta acatttttta aatagacatc tttggtaata
     2701 atccaaaact tacgcatcga tttcacctgc tttcaatttg aaaatctctt ccaaagttgg
     2761 cggttgctgg ttaaacatag gaatgtagcc gaactgagtc gcacgagtaa agatttcttt
     2821 cccagcttca ggatcttcta aggtaatctc taacgtattg tcttcatgaa ggatcacttt
     2881 tttcacgcct tcgatttctt ctacttcctg ttggcttaaa ccagattcca agaataactt
     2941 cgtacggcca aatgattcac gaatctcatg tacttttcca ttcaaaacca tttttccgtt
     3001 tcgcaacatg atcaaatgat cgcagatttt ttctacatta tccatattgt ggctagaaaa
     3061 aatgacacat gaaccatgtt ttttcaattc caatattcca tcttttagca attcagcatt
     3121 taccggatct aacccgctga atggctcatc caaaatgatc aatttaggtt catggattaa
     3181 cgtggcgatc agctggactt tttgctgatt tcctttagac aaagatttta ctttatccgt
     3241 ctttttccct tttacctgaa atttttccat ccaaaaatcg atttttggtt caatctcttt
     3301 tcttgatttc cctctaagtt ctgcaaaata aagcagttgg tcttgaatcg ttactttcgg
     3361 ataaagtccc cgttcctccg gaagataacc gatgacatca taatctttct ccccaagatg
     3421 atggccattc cataacactt ctccttggtc ttgtgtcaaa aaatccaaga tcaaacgaaa
     3481 agtcgttgtc ttcccagccc cgttttggcc aattagcccc atgatttttc catcggggat
     3541 ttggaaagag acatgatcga cagctgtgta gttcccaaaa gttttaacaa gatccttgac
     3601 ttctaacatt tcttcactcc ttcttcaaga aatcacatat atccttagac accagacgta
     3661 agcgatttat tatttaccaa caaaacgtaa acgctctttt tgttgtacat aaaatgatag
     3721 gacactttac tttctttgta aagtgccctt tcctcattcc tgatgtaatt tatcaaatac
     3781 gttttgtgcg acatttttcg gcagtccgat ttcttgtaat tcttctgtcg tggctgctgt
     3841 gatatttttc aacgatttga actctttaag cagcattttt tttcgttttg gtcctaaccc
     3901 ttcaatttca tctaatcggg aagcaaaact gtttttactg cgtagctgcc gatggaaagt
     3961 gattgcgaat cgatggactt catcttggat tcgctggagt aagaaaaact ctggtgaatt
     4021 tcgctcaagc ggtactacag ccaaatctat accgaatagc aactcatttg ttttatgttt
     4081 atcgtttttt gccatgccag caataggaat atcgattcct aattgatttt ctaagacatc
     4141 ttttgccaca tctacttgtc cttttcctcc atcgatcaag atcaaatcag gtaaaggcaa
     4201 cccttcgcga atcactcgtg aatatctgcg atagatgact tctctcattg atgcataatc
     4261 atccggtccg ttgactgttt ttattttata tttccgatat tcttttcttg ccggttttcc
     4321 atcaatgaat acgaccatcg cagccacagg attcgtcccc atgatattag agttatcaaa
     4381 agactcgata cgtattgggg tcgggatatt catcgcgttt cctaaacgtt caactgctcc
     4441 gatcgtccgt tcctgttttc tggcaatcag atcaaaccgt tcattcaaag caactgaggc
     4501 attcttattc gccaattcaa ctaatttttt cttttctcct cgttttggtt gcagcacttt
     4561 cgtagctaac aaagcttcta cactgggctt gtcgatcgta tcaggaatca agacttcttt
     4621 aggaatgaaa tgctcatttt cctgataaaa ctgaccgagg taagtcaaga aatcttcttc
     4681 tgcttcgtta tagaaaggaa acattgaaac atctcgttca attagctttc cttgtcttac
     4741 gaaaaacact tggacacaca tccatccttt gtcaatcgca taaccaaata catctcgatc
     4801 cagtaaatca gtatttgtca ttttttggcg agtcatgatt gtctcaatgg cacggatctg
     4861 gtctcggtat tctgcagctt tttcaaactc catgttttca gcagcttttt ccattttctg
     4921 atggatctct gactcgattt tttcgtgtcc tccgtttaaa aactttttca cttcatcaac
     4981 gattttcgta tagactttcg gatctacgtc gaaataataa ggacacagac attgtcccaa
     5041 atgatagtaa agacaaggct ccttcgtctg actaggtttg cattttctca gaggaaataa
     5101 tcgatcgagg atcttctttg tctcattcgc cgcaccgaca tcgggatatg gtccaaaata
     5161 cagtgctttg tcttttagta ctttacgagt aatcaccaaa cggggatatt tttcattcgt
     5221 tatcttaagg aaaggatagg ttttatcatc ctttaacata atgttgtatt taggatcatt
     5281 tttctttatc agattgatct ctaagagcaa tgcttcgata ttcgattctg taacgatgta
     5341 ttcaaagtct tcgatttcac taacaagccg ttccgttttc gtattatggc tgccagtaaa
     5401 atatgagcgg acacgatttt ttaatatttt ggctttgccg acataaatga tcgttccatt
     5461 tttatctttc atcaagtaac agccgggctg atcaggaagt aaagctaatt tgtttttgat
     5521 tcgttcgttc atctggtact ccccttcata ttcaattaaa gttattatag catgtctgtc
     5581 aaaaaaacaa tccagcctga caacgtaatt atactcaaaa aatattaatt tgaataacct
     5641 taactcaacc gcaaaaaaac cgaggacgga actggattca gtcccaccct cagcttattt
     5701 ttgctgtaag tttttataaa tattgatcga tcatttgacg tagttgttct tttgaatgaa
     5761 caccaacagc tttttctact acttgaccat cttttttcaa cattaatgtt ggaatgctca
     5821 tgataccgaa ttgttgagga gtttctggat tttcatccac atccatttta gcaattctga
     5881 attcttcttc atcatattct tgttccaact gatccaaaat cggtgcttgc atacgacatg
     5941 gaccacacca agttgcccaa aaatcaatca atacaagtcc tttatctgtt tcttcattaa
     6001 atgtcttatc tgtaattact tgtgacatat caaagacctc catttttaaa atctatgatt
     6061 agtatagcat ttctatactt ggatagcgaa ccatctgctt acctttagta agctttactt
     6121 aaatttaaca attgtcgcac catttccgcc ttggtttgcc ggtgcaaact caaagctttt
     6181 gacgctccga tggtttttaa gataatcagt aattcccttg cgaagtgcgc ctgttccttt
     6241 tccgtggaca atagtcactt gaggataacc agctagaatg gcagaatcca aatattgatc
     6301 tacctcactt agcgcttctt cgtatcgttt tcctcgaagg tccaattgat tcggcacgtg
     6361 actactagat tcagatcgta cagcatggat aactcgttgt gtcggctctt tttgaggagc
     6421 aacaggcgtc agctcgtctt cggacacatt catttttaat ataccgattt ctacttgcca
     6481 ctgattcccg tttttacgta aaagcgtccc tctttggccg taagtcgtaa ccaacacttc
     6541 gtctcccggt ttcaacgttt tttgttcttt cgccttcttc aatactttat ttttcttcaa
     6601 agtttcttct tgatgtagat ttgctaattg ggtctttgca tcgatcaact gatgctcttt
     6661 cacatttcct tgcccaatct gctgttgcat tttacgaata tcagcaatga ttttttctgc
     6721 tttttcttcc gcctctgaca cgacttcgtt tgcttttttc tttgcttttt ccatttcttt
     6781 ttcacgttct tcaaagaaat agctgtaagc ttctttcaaa tcatcgtgaa gttcttgtgc
     6841 ctcagaaaca aaatgacgca tttccagata ttcggtctct gccattttcc ggcgattctc
     6901 taaatctgtg atcatttcgt ttaaatcttg gctctcatca ttcatcaact gcttcgcttc
     6961 atcaataact tctgtatcaa gacctaatcg gctagagatt tcaaacgcat tgcttcgtcc
     7021 aggaacgcca atcagcaaac gatacgttgg acttagtgta tccacatcga actccatgct
     7081 ggcattaatc gtatttgccc gattgtatcc ataaactttc aattcaggat aatgcgttgt
     7141 tgccatcaca taagcagact ttttaccaag atcatctaag attgcgattg ctagagcagc
     7201 accttcttgc ggatctgttc ctgcaccgag ctcgtcaaat aataccagac ttttttcatt
     7261 gacttttgat aaaatatcta cagtgttcgt catatgagaa gaaaaggtcg atagactttg
     7321 ctcaatcgac tgttcgtctc ctatatccgc aaatacttcc tcaaaaatac cgatttgact
     7381 ttcttcacct gcaggaatcg gtaatcctgc ttgccccatc agttgtaaca gacctaaagt
     7441 cttcaacgtg atcgttttcc cacctgtgtt aggtcctgta atgacgatgg cttgatagtc
     7501 ttttccgatt gtgatgtcat tagaaactac tttttcctga tcgatcagtg gatgtcttgc
     7561 ttgtttcaag acgacatgat tgtcttcact gattcccggc acgatggctt ttacttcttt
     7621 accaaatcgt gcttttgcat tcatcagatc cattttacca atcacataag cattgtggat
     7681 gatttcttga cgatggggaa caagttcagc agaaagttca cttaaaatac gttgaatctc
     7741 atttcgttca gcgatttggt attgacgcaa acgattattc aaatccacca cttgttttgg
     7801 ttcaataaat aaagtctgtc ctgaagcact ttgatcgtgg actacaccgc caaatacacc
     7861 gcggtattct tgtttgactg ggataacgta gcggtcattt cgcatggtga tgatcgcatc
     7921 acttaaatac tttgcatttt tcccacggac gatcccatct aactgttcac gaacagcttg
     7981 ttcacttcgc cgaatgttct gtcttatgct ctttaactca ggagaagcgt catcagtcac
     8041 gcgtccgtct tcatcgattg cttcctttaa acggcggctc aaaacaggga tcgtgactaa
     8101 ctgatctgcc cacatataca aacgtgcaaa ttcgatttca ctgtcagaaa gatcatcaat
     8161 gaaacgattc aattcgcttg tcgtagacaa tactctggaa acttgtgcaa gctccactcc
     8221 attcaagtct gctccgatct cgatccgttt catatgggga cggatatttt caagtttagg
     8281 tatagggatc ccgcctcgca gacgttgcac tttcaatcca tcctcagttt cttgaagcca
     8341 gtttgtgatc gtttgtttct cactcactgg taccaattct gccagttctt cttttccttg
     8401 tgcagtcact acaaattgtc tgaccatctg tttgactttt tcaaattcca atgtttcaag
     8461 gattcggtta ttcattttat caccttatct ttcactatga aaaaagagct gtgccggaca
     8521 tacgctacgc agccttctta aataaactac tctgtacaaa aaacgaaata ttttccagca
     8581 ctattttcct taattcaaaa tattcgttac caaagctcat acatcttgtt ggtaagaaat
     8641 ggcgttctct caacgataaa acgagcgaaa ctatgctcgc cgaattgatt ttggaccaca
     8701 tctaatggaa caaaggcaag taactgcaaa atcaaaaata tagtaacgta agccatgatt
     8761 agtgacagga tgcctcctgc aatccagtct acctgtctca agatcggcac atacgtcaat
     8821 ccatggacaa aaacccccac aaatcgggta agcagccagc caattagcaa aataatcaga
     8881 aaagcagttc ctgcataaaa agcttcatct aggtgaaaag caaatacctg atcaaaaaat
     8941 gctaactttg atgttggggt gacagctggg tatggaatgt ataattccaa atgacttgct
     9001 aatggtttgt aaaaatgctg ggcagcaata aatgacaata catatccaat cgcgaatatg
     9061 atttgcaaag caaacccgcg acgtgctcca gaaaaaaacg caattagcaa taaaaataaa
     9121 atcagcaaac tcagcatgct acgttccttt ctcttctaac ttttttaaat ttttcttatt
     9181 ttgataaaag gatcttaatt agaagatttt tgtttaattt gtccttttcg tttctcgttt
     9241 aaaatctgtt gtgcttccac atgattttta atggtatgat ctgcttgacc attttctttc
     9301 aatacttccc gagcttcgtt ctcgattgct tcaatgcgtt tcacacgatt ttcaagctct
     9361 gtaaatttga tcattttttt ctttaattct gctgtttctt cttccagctc caagattctt
     9421 tcttgtttct tcaattgatc cgaaagcgca ttgacagcca tcaagattgc tgcttgttca
     9481 ttatccattt gtggtgataa ttgtttcaat tctgctaact gttcattgat cagcttcgtt
     9541 acgatatcca tatgatgctt ggtttcccgt ccgataatag tatatgtttg gttggcaatc
     9601 actgctttgt agcgtgtttt ttcgtgcgcc atacgaatca tttcctccta tttgatgaaa
     9661 gttttattta gtgtaccatg aagttggttc tttcaccgtt ctccacaaat atttttcaca
     9721 tacttaacta ttatatgata aactttaaaa aaatgctatg tctcgcaatc tcttagaaaa
     9781 cgaaaagtcg tgagttgcgg tatagtttta tgtataaaaa cgaggagaat tttatgagca
     9841 acgtcattat caaagcaaca aacgaccaac ttagtcaaat gaaagcatac tatcgctctt
     9901 atttgttaaa taaacagatt ccttatacta gctttgctgc aaaaaaagac gggacgacca
     9961 ttaccgctta tacatccgga aaagtgatgt ttcaaggtac gaatgcagca atagaagctg
    10021 ataaatgggg ggaggcacag gataaacaag ccaagccagc aaaaacagcc tcttctttgc
    10081 caaaaaatat cagtcaatta tccgttttag gtagtgatga ggtcggaaat ggcagctatt
    10141 ttggtccgct aacagtatgt gctgcttatg tgaaacgtga tcagcttgca gaattacgta
    10201 aacttggtgt tagagattca aaagagttaa aagatagcca aatcatccag cttgctgaca
    10261 tcttaaaaaa aaccatcccc tataaactgc ttgtcttatc tccagaaaaa tacaatgaga
    10321 tccaaccaaa atacaatgcc gttcggatga aagtcgcttt gcacaatcaa gcgatccatt
    10381 tgctgctaaa aaaaatcgag ccagaaaaac cagaagcgat tttgatcgat cagtttacgc
    10441 cggaagcaaa ttttaaaaaa tatgcgcgtt tagaaaaaaa tcaattacaa gaaaaactgt
    10501 acttcgttac taaaggagaa caatatcatt tagctgttgc tgctgcttct atcatcagtc
    10561 gtgccagctt tttggaagag ttacacaaag catcagctga agctggaatc acccttccat
    10621 caggagctgg aacgaaatct gaccaagtag cggcaaaact attggagaaa ggcggcatgc
    10681 ctatgcttga aaaatatgcc aaacttcatt ttgccaatac agaaaaagct ttgaagctgc
    10741 taaaaaaata atggaactgt gacaaaagtc atgtcacagt cccttgttcc gaataaacgg
    10801 tgttctcatg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg agaagctgtt
    10861 ttttgaaatt gctccttgtg acgcggagtt aaacggcagg ctcacaacct cttccttaaa
    10921 cggtgttcaa aagcttgcat ctgcgataat aagcccggcc aaacgaaaaa tatgaggagc
    10981 tatttttgtt tggctgggct tatttattac tgaaaataag tggtcaatgc ttgtgtcaaa
    11041 cttgcagcaa tctgctgctg ataactgtct gttactaatt cctttaaatc actatcatta
    11101 ttcatataac ccatctcgat taaaatagaa ggttgaagat tttctcgaag aacttgatag
    11161 ttcccagtgg agattcctct tgaatccaga ggcagatcag tgaaggagtc agcaattgtc
    11221 tgtgctaatg tttcctcttg tccatagtaa taatatgtcg tgattccagt tgcttcattc
    11281 gcatattccg tagagtctgc gtgtaaactg ataaagacat ctgccttttt ttcttcactg
    11341 tagtcgcata tatctccaag ctgtactaat tcatctgtag tatgggtcaa ataaaccgtt
    11401 gctccagcat cctctagcgc cttttttact tttatggctg tacttaatgt tatatctttt
    11461 tcttctgttt gttcatcatt gcttaatgcc ccagtatcat ttccgccatg cccaggatct
    11521 aatacaatca ctgcatcaga tagctcttcc ggttttgttt gtatattctt tgtgactttg
    11581 gttccatctt cgagggctaa atatccagta tagccatcat ttgtcttgat ctgataagca
    11641 ttttttgttt cagaaagaaa ctccgccagc tctccgccgt ttatagttgc aagtggggta
    11701 gttagcgagt catctgtata tagtgtacgg tcattttttc cgattttgat taatgtctct
    11761 tgttctgtat catcaaatga ttcctttgtc tttgtaaccg tactgttcgt actgctgacc
    11821 gtttgtccaa ctatcttttt ttgaccaaaa acatctattt gaaaacagat aataatgata
    11881 atgattgcaa atacaaaagc tccaattatt tttttcattc ttgactctca cctcgtagat
    11941 ccagactaag ggtcaaaact taaaatcagc ttaagtacgc aaaaaagggg ctgtaaaaga
    12001 tataaccgta gaaaataaca ttactgctaa aatgattttt ttcatttttg gcatatcctg
    12061 aaatctcctt ttgactattc gtggtgattc atctacccaa taaatagttt ccttcattct
    12121 acaaacaatt aatttcttcg ttattaaatt aaaacacttc ctggaacacc gacataataa
    12181 ataaaaatat accctgataa tcatgagaga ataacaaatt tgtatttatg aaaaactacc
    12241 tatataaaaa aaacaagata taacgatctt cgtcatatct tgcttcttca cctaactatt
    12301 tttctcccta tataacagct ttacgcttct cctaatacac cattcaaata taaatcgatc
    12361 aattgcgcca catttagcgg ttcttccaca ggtcttttcg accgagtgat gtaaggtgct
    12421 aacactaaga aaaaatgcct tgctgcgatt tcttttgaaa tcccatttct tagcacagat
    12481 tggtttgctg aaaagatttc ttcaaaaggt tcacgataat ttttcttcca tagaaagaaa
    12541 agatgatctc ttgtttcctt tgaaagattg ctttcaagat cgtgaagcat cagaaacaaa
    12601 tcgaccagat gtgtctcaat caaaaactgc gccatttttg tcaaggttac ttgaaatccc
    12661 gtttcgcttg tttgaacgat ttctctcaaa gcttttccaa catcttttaa gtggttttct
    12721 aaaactgccg tatatagttt ttctttatca ccgaaataat gatagagatt gggttgggtg
    12781 atcccaactt tttgtgcgat ctctcttgtt gaagtgccga gatatccctt ggacataaac
    12841 aagtcagtcg cttctttcaa aattttttgc tgtgtatctt tgttgtatcg atggaccatt
    12901 tttccacctg ctttttggtt attctttttc tttcaataca ccatcttcca tcataaatat
    12961 tctatcgcat ttgtcaatca gccgtgtatc gtgagtaacc atgatcgttg ctttattttt
    13021 gtcttttgat tcttttgcca agatatcgac tacttctcgt gccttttctg aatccaaact
    13081 agcagttggt tcatctgcta gaataatgga cggatcattg tacaatgcac gagcgattgc
    13141 gaccctttgc cgttctccac cagatagttc ttctggatac ttgttgatca acttatccac
    13201 gccaagctgc tcaaataatt cttttgcttc tttttgacga tgttcatgtt tgatcttatc
    13261 tactaataat aactgctttt ttactgtcag aaaaggaacc agattggaag cttgcaaaat
    13321 aaagccaatt tctttaaaac gtagtttaac gcgttcactt tctttttgat tactgaattc
    13381 ttgatcattg ataaatacag agccactgct aggcgtctgc aatccgcctg ccaaagtcaa
    13441 aaaagtactt ttacctgaac cagaaggacc gatgattgca acaaattccc ctttatcgat
    13501 actcatggtt gtttctttca atgctttaac tacttggtcg ccatcttgga aacttttttc
    13561 tacttcttta aattctattg ctttcatgct cttcctcctt cttatccaat tgccttcaac
    13621 ggatcgattt ttacgattgt ccgaacagaa aatagtgcgc ctaacacagc tactaccagc
    13681 atcaacaagc taatgacgcc aaaaaacagc caattgcttt ggaatggaac cgcatcaggt
    13741 agaacaaggg atgttcccac tgttcctaat agaccaagaa gaataccaac aaatgataaa
    13801 atgaatgttt gtgccactac agaacgcgca ataaatcctc ctgtgatccc ttgtgctttc
    13861 attacaccaa agatattaat cttttgcatc gtcagtacat aaatgaaaat cccgatcaca
    13921 atagccgcaa taacgatcaa aaatccgatc ataaatccaa aagtcaatac ttgcgcatta
    13981 tatccaggta attcattgat gaatttagaa attttgatct gttctaaatc ttcaggtaaa
    14041 ttactgattt tacctctgac aactattgcg ttaatccgtg cattttcact tgtatcttct
    14101 ttttcgaagc ggatctcttg gtaagcattg atggttgtat agagaactgg tgagacattg
    14161 aatttggcat gatccgtaaa gccagttagt ttaaacgtct tgtcactacc agataactta
    14221 actgtatctc ctacggctag accatattct tctttcaaac taatatcgcc tactgcctca
    14281 tcatcgttat caaaagcttt tccttcaaca agattgggca tgatgaattg atttttatcg
    14341 ataccgaaga agctaacatt gatttttcct tcttctgtac tgtctttact agtcacgact
    14401 ccaggtgtct gggcaagata cgccactttc ccaccttcga cttcttctgc tatcttctta
    14461 gtaatcatgg acatccctag atttgaattt gcatctttcg aaagcacaat cgaatctgcc
    14521 tcccacttgt cgactgctgt tcgattatct tgagctaagc cataagctaa cccagtcaaa
    14581 aaaaatacca ggtaggcgat caaaaacatc acccctgcaa ctaacgcata acgtaacttt
    14641 gaatgcataa tttcatttaa tgctaaaaac atttaactcc tcctcctgaa ataatcattt
    14701 tatacaacaa ttttatcact tgataaattt ttggcgaata ttatgctcaa aaaattcttc
    14761 cgctatttca ttatatgaat tttttattct cgattgatta atatttatta tatccccaca
    14821 caataaaaga ccgtgatatt tctcacagtc ttttatctac tctattgcta tttgctctct
    14881 tagctagtac tttaaaagct aactcattga ggtagtgaaa acgagaggtg tgttcagaca
    14941 gaaatccaaa atcctcaact gctagctgtg gaatcgtagt tttgtgtttc acgatatccg
    15001 caagcaggtt caaatctgat aataagaatt cttggcttat cagctgtccg ccaattattt
    15061 ctaatgtatc cggatggtag atcagctttg catttactgt tttgttttct tcatcgtatt
    15121 gtggaaatag atcaaattct cctgaacacg aaccggtatt ttgttccaag aatggcgctt
    15181 catcttctgt cagccctaca gttcctaaaa agtagtctat aatgactgct gctgttatct
    15241 tttgtctgag gtcgtaagcg gttggctgac cactcaaagt tagcgctact gctcttgcca
    15301 tatgtacagc tcgagtaact aaaggtaaaa agagatgccg tttatttttg tcaaaactga
    15361 ctgggacgat atctccaatc gcatagatgt ccggatcact tgtctgcaga tattcattga
    15421 ctatcacagt ctcatcctca tacaaagcca ctttttcttt taacaatgtc gtattaggac
    15481 gagtttgggc agaaaaaatg accatatctg cctttattgc ttgagataga agttgtacgg
    15541 atactacttt ctcttctgat tttttccaat cgatcaagaa ttcatttgga taaaaattga
    15601 tttgcttagt ttgcagctga ttttgaaaac tgttgatcat atccttatcg aaatatcttg
    15661 ataaaacatt cggcatataa tcaactagat gaataatgta tcctttattt ctcaaagcat
    15721 ctgctaattc caatccaata taaccagcac cgaagatcac tacttctttt gcttctttta
    15781 aagcggcaag tacttgctta gattgcacta gatttttata ggtgtagaca ttttcagctt
    15841 caatttgttt atacaaggtg acaggagagg aacctgttgc tacgattaat ttatcatagg
    15901 ttatggtctc gttttttcct tctttggatg cgaggatgag ctgatgtttg tctggatcaa
    15961 gttcagttac tctccattcg gtgatcagtt ttgccccact ttcttgaaga gtccgcatgt
    16021 tatgggcaac ttcagctaaa ctacgtatct ttccttttaa ataaaggttg attccatttg
    16081 aaacatagcc tagtccatct ttatgatctt catcgatcag taaaacttct gctttcggtt
    16141 gaagttttct tagatttagg gcagctgtga tgcctgcatg ggaagcacca atgatcacga
    16201 ttttcatgtt ttattcatcc ttaaattcaa tttttctaaa taacattctt ttaaaaattg
    16261 gttcaaatag gggaaatcat aattatattc gttgctagtg aatacaaaaa ctttagcgga
    16321 cttctgtgac gggtactctt tcaattggct agtcaaaatg atatcatact gcttttcttt
    16381 ctcgtattcg actaaatcat atttgatcgt attcagcaat tgttcctgga tgatttcttt
    16441 cacgatatcc gccattagcc tttcataagg aagatcataa gcaatttgca gacgtaattc
    16501 taactgcttg atcagcgtcc gcaaaataga tgcatagcga gcaaataatt caaagcgggc
    16561 attttcctcg tctacttcag taaaatcaac aatagcaaat gtttgtctga ccaatctttc
    16621 acataaaacc aggaattgtt tatcttctac ttcagttaac cgctgtttta atccttcttg
    16681 gccaaaaata ttcatccatc ctctaaaata ggcaaaatga gcgtgatttt gcatcactgt
    16741 caacgcaatc ttttgcgaaa tctcttctgg aaaaacataa aaagagaaat attcttctat
    16801 cgattgttta aacgttcgat tcaattgagc tactacgtcg tctttgtatt tttcgcttat
    16861 caaaaactcc tggacatgcg agttatgtgt atctaatgta aagttactga tcatgaaaag
    16921 ataaaaaacc aacgtttcat tttgattcca aggaaaggca tattgaaaga agtaacgggt
    16981 caaaaatgta tggaggtcca ataaaaattg tgtcgtttga aacaatccaa caggaagatt
    17041 gctataatct ggtttggatt ttttcgtaca gcgcattcgc ttcttcataa tggaaagcca
    17101 tatttcaaat ttcaatcgtc cttcttctgt aatagtaaaa ctaaactctt tttgcagcag
    17161 atctaaaaaa gtgtttcctg ataaatcact gtattctttt ttgttttcaa gataaggacg
    17221 tccaaaccaa aataattgga aataaaaata acaaatttga ctttctggac cggtcagccg
    17281 cccgcttttt attttcagat gaaactcatt taaagaatca tttaattcct tcactctgcg
    17341 agacaaagta gccgaactga tattaagttc catgacaagt ttctgccgat caaatgagcc
    17401 attttccaac agataaagaa gtatctgata ttttgtagaa tagcgcaagt agcaatgata
    17461 ataaacatcg actgctgcta tatttccatg tctgattaat aaaatattcc cttcttggat
    17521 aacaatttcc aattgtttga tattttccaa gttagcttgt aaataaaaca gatcttcttt
    17581 caaggtagcc cgatcaactg ataagcgggt aagtacgtta ttaacggaaa tctctccttt
    17641 ttcctctaat aaaagttgga aaagtttgat ttgccgttgt tcattttttt caagcaaact
    17701 gaaaagttcc aagtccactc ctccaagcat ttctgatttt aacagcagaa gtttccttct
    17761 catccttgaa aaagaggatc tgtgacagtt atcacagatc catacaaatt ctaatcactt
    17821 agctggttgg cccagctgtc agttcccatg tgaccatgcc ttgatacgtt gcgccaggtt
    17881 gagcattcgt actgctattt acctctaaga gtaaaccatc ggtatcggga aagtcaacga
    17941 gaacctctcc tttagctgtt ccatcactct cataaatcaa cgtgttttga ccttgctgaa
    18001 tcaattgttt tgatccagaa tgattgaagt ataattttgt tccgcttaat tcttgacctt
    18061 gatcattctt aaataaagaa gtcaatgtcc ctgtcagttg ccaaggagac gtgcttggaa
    18121 gccgagtatc ccgcacatca aaagaaaaat cgccttttgc ttcataataa cttgtcttca
    18181 acggtttggg gagatcgtta ccaaatgtaa aatcatctgg tacattaact aatgttaatt
    18241 cttgtttggg gacaagaatt gttttagcag atgtttggac aactgggtta ggaagcttag
    18301 caccatctgc taatgtaata ttgctggaaa gtacagctgc ctttgtcagt atgctgacgg
    18361 attgtacaga agtatctaca gtatttaatt gatagttgta agtaagatga atggtatctc
    18421 ctattttgct caatagatct gctgggacag aaactccttg ttgaatgccg gtatctgcga
    18481 tagttgtacc atttaatttt aaggaaccat tgatataagt agtattttca ggaagtgaga
    18541 tcgtgatttt tttgtcggtt atcgcatccc cagattttgt taacgtgaat gtcacatttt
    18601 cttgaatcgt atctcctgat agagcagaca ggggatctcc tgtctcaggt tgtctgctaa
    18661 ggttttttag ctgactatct gcactcacag atggaaaatt agccactgtt atttgaggaa
    18721 gagtggtgat tccagcttgt tcagactgcc atttgtacgc aaaagacgga tacatgttag
    18781 taagtacatc gtccaccaag cgataatcaa aataatattg ccctgaagcc aatgcaggta
    18841 gtgtagaagt agctgtattt acacccccgt tatttgattg gatcgtttgg aaaagttgat
    18901 ctgtccatcc tgtcgctaat ttttaggttc ctttcgaata aagcctgtaa tggatcgaac
    18961 ccttttttag ttcagttgct gaccaattgg ccgtgacttg gtacgatgca gaagaaagga
    19021 gctttcccca ctgactatcg gctgttaagt ttgttggtaa aaccgttccg tctagcttgt
    19081 actgctccaa tacctttaaa gcaacaggag aaagcaatcc gccttgcgca tctttcccac
    19141 tgtacacgat attggctgtc tgattcaatc cacttgtgct tgctggagta tgagtcagta
    19201 ttgctccttg tgtaacgttt tttcctgtaa aatagttgac tacgcgatca aagcgactaa
    19261 atggcaactg atcaaaacgt ccttcagtca gccccatcgt caatgtccga ctattggtag
    19321 agcctggaag gttctggtta tttccatcca cgtattctgt ggtatcagca aatgtattat
    19381 attttcctgt tgaatcactt gttgagatgt aactattcca cgtatttatc tctgcacttg
    19441 cagaaaatgc atatgctcct ggattcaagt ttccgccagt taatttagga taaaatctac
    19501 ctgcagctgg agctaaaatc gaattgaaca tacgatcttg gataatatta ttcgaatcat
    19561 aaaaatcaac agaaaaaacg gcctttttag acatagaagg ttgtaattta cccttggtca
    19621 aaattttgtt tggactcgta tcatatttat ctccgttttc tgtcatcttc gcgacattag
    19681 ctgtaacatc aaattgaagc gggatatcgt ccacgttgaa ttcgcctcgc gtaataatag
    19741 caataatatc tgccaataga gtatttgtat cccacgaccc catcccaaaa cgcaaggtat
    19801 ggcttcctgc tgataataaa ctaccgctag taaccttgat gggggcaccg gcaatataca
    19861 agtaggcgtt gttccagtca attgctttta atacatcact gcttgataca tgttccggca
    19921 aattgatgat cgccacaaat gcaaatcctt gtgcagtgat attcaccaaa tcaagtacat
    19981 ttgttagttt cacatggctt tcccaatgaa aacggaccgt gccacctttc caaggatcat
    20041 ttgaaccatc gttcaaataa gtagcagtca atttaggcaa ttggttagcc ccagttagtt
    20101 ggacaagctt cccgccattg actgcactac gtccaccatt tggaggtacg atattttcat
    20161 acgtgtatgg atcataagaa atggcaggag gcgttgctcc aacagcttct gccgaaggtg
    20221 aaaagataaa tatacttaaa acaaaaagcg aagtaaaaaa gaaggagcga aaaaatgaaa
    20281 atttattttg tagcaacatg ttcatcctcc ttttcctttc ttctccataa caacaaccac
    20341 gcaagaagcc ataattctat accaatgatc aaataaagaa aacggatctt ttcttcattc
    20401 gtctccggga atacgccatg ttgattagat tctttataaa tcggtagctg tgtctgctgt
    20461 tttgatgcgc cattgactga accacttgga tgcggaactg gacttagatc ataaaatgta
    20521 atcgtacctg aacttttggt atctgcttgg actggctctt gccagctgaa gaacagcaaa
    20581 cttgcaataa tccctcccaa taagatcgat gttttcaacg aaatcacctc atcatgttgt
    20641 ttctcctttt ttctttttct tgtggaccca caaaaacagc aaaatcaacc ataacactag
    20701 aactgcagca accaatcctg cataaagcca tgggaaagat tgttcttgta ctgcaaaaac
    20761 ggaattttca tttacttttt gtgctttttc ttttttgatc gtaaagtttt gtgtaaatcg
    20821 ccattcatta ctgtcgcctg attttgctat gatttctgcg cggtaatcgc cggcttttgc
    20881 tttatcacca gctaaatcaa gattcaagtg aaaattcgaa tttggtgcaa ctaaaaattc
    20941 attagacgta ttttcaatat aaagctgatt ttcataaaag atcttgctcg taaatatcaa
    21001 attgctgata atcctaggct gaggattttg gaaacgaagc tgaacagttg gctttgcgtt
    21061 gacctcgtcc aaatctcctg ataaaagttt cagatccggt tctatcgtct cttgactttc
    21121 ttgtaaaacg actgcgagat tataagcaat acgatttttg atttgtgctc cttctgtagc
    21181 attttctgtt tcagcttctt ctttaggtga aacatgaata cccgctaata cacgtccctt
    21241 aaagcttttt gctgggattt tcacttctat aggtatagtt gtttctgcat gtgctgatac
    21301 attgatttcc tttttcgcaa tgagcaaaac gtcacggaca tcaaacggca atgtctcatc
    21361 taacgttggg tttttttgac tataatcgat cgttccacca tcactcgtta ccgccggatt
    21421 gacagagacc tcaaatgttc gttcttcatc tgaagaattg ctgataatta cttctagtgt
    21481 ctgattttgt tccggtgcca ctaataaatc aaaataaccg attgctttat ctatttggtt
    21541 ctctggaaat accggtttga ttccaaagtc tcctgctgaa tcctttgctt ggctgattga
    21601 cggaaaaatg aaaaatagac ttaaaataga aaaaaacgaa acaaacagtc gtttactttg
    21661 attcattgtc caccacatcc tttcaacaac aactgcggta aaaaatcctt cacctatagt
    21721 attttcaccg cagtttgttg aatttatttc atactctatg ctactgggcc agccacgagg
    21781 ttccaagtca aatcagccgt gaatgttcca gctgctgctt tcggcacttt cagtgtgata
    21841 tttgctggat cataaacaga aagccatgtt cccagacctt gatcttttgc tgctgtaaat
    21901 attggtgtcg ctgcacttgc tttactcaac ccagttacag tttgactagt tggtccagtg
    21961 gattcgttat ctgacgtact tttaacagtg gatgttccaa aatctaattc agcgccttgc
    22021 aaaacttgtg aaccgtttac aaaatctgaa accgaaactg tcacgttcca cccttgtgct
    22081 tggccatctg cacctacacc tcgacgatct gatacttgga gataaggtgt atcgtttttt
    22141 gaagcgtcta cttgatacgt ttttgttccg ctttcaatct cgtgtgtacc aaatggcaat
    22201 tcaggaacta catccaatgt taaagaacct gtctggccag ttgctggatt ttccggatct
    22261 gctggctcta ctggttttga aggatcagta gggttaagag gctcaacggt atttgttggt
    22321 tcagtaaagg taatatctcc ttttgaagtc gcttgaccct cagcaaaagc tggtgttgcc
    22381 aagccgccta gaagaagtgt tgaaagtaat aatgtcccaa tcatgttctt tttcataata
    22441 aatcactcct ttttcttagt gacctaagta tagtagagac agtatgcaaa aaatcctcat
    22501 gaattcatcc gcttacttga aattatgacc ctttttgtat aaaagagaaa aaaacaataa
    22561 aatcgtttac atgaatattc ttcctttttt attaagtatg aaaaaatatt tcaattttat
    22621 tttttcgttt caaattattc tattagtgac atccttgaac tataaaaata ggagtacggc
    22681 atcatctgcc gcactcctat ttttttcgtc atttatttct taaataaaac gattctcacg
    22741 caattttatt tttttgatat cttctactgt ttgtgttcca gctaattgca tgaccatttc
    22801 caattctgtt ttaaagaaat caaacacttg atggactcct gtacttcctc ctaaagacaa
    22861 tccataaata acgggtctgc caatagcaac aagatcggca cctgatgcga ttgctttgaa
    22921 aacatgttgt ccacgtcgta caccgctgtc aaacacgatt ggtacacgtt tatctactgc
    22981 ttctgccaca tattgcaacg aatcaaacgc agcaggcccc ccatctaatt gacggccacc
    23041 atggttagat acccaaattc caccagcacc agattctaat gatcgataga catcttcttc
    23101 tgattgaaca cctttcacgt agactggcaa atcagaatga gcagcgataa attctacatc
    23161 ttttggacta agtttttgtt tggaagattt atatacagca tccatcgttt ggcctactcc
    23221 tgattgataa gcctgaacga tcggcattgg cagcgggaag gtaaacccgt ttctgcggtc
    23281 tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat acgattgctt ttgctccatt
    23341 acgcttggcc acttcaagaa tatccaaatt gattccatta tctttgctca tataaaattg
    23401 aaaccattgc ggtgctttct ccccgcctgc ttctcttatc tcttctaaag tacaagaagc
    23461 gtaagaactt gctgtataga tcgtcccaaa atctgctaca ccttttgcag aagctttctc
    23521 cgccttcaca tgagctaatc catgagcagc aactggtgcc atgatgattg gtgcagtcag
    23581 catttcttcg tcaaaatgtg tagttgtatc gggtaattct acatctctta atacatgagg
    23641 aataatcaaa cgatgattga atgcccgttc attttcttga taagtaaata agtcgccagc
    23701 accactgctg atgtaaccat aaccaccagc tggaatcact tgtgaagccg cgatttctaa
    23761 atcttccata ttgataaaat ctactattcc ttcatttgta cctgcttgat atgttttttc
    23821 catctcaatg ccttctctct ataaaatttg aatgatgaac aatatgatag cgtttacact
    23881 ttttatatta ctcttcgaca ttgatacagg caaataatct gtttacattt cagccattcc
    23941 agatgtcact actctggaag aaatgattgt aataaaaata gataaaaaac cgatgggact
    24001 ttgacaaaag tagtgtcaaa gtcccttttt ttgaataaac ggtgttcaaa agccattttc
    24061 tcggtattaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
    24121 ctactcgaaa ctaagcgact ttttcacaac ctctcgataa aacggtgttc aatcagctga
    24181 ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc
    24241 taatactcag gtcagaacgt tgacttcaca gtctcttcct taaacggtgt tcaaaaactt
    24301 gcatctgcgg taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg
    24361 ttcttattac ttgatgcagg gcaggttttt cacgacctct gattcacggt gtttcttttt
    24421 catcaacatt tttacgaaat actgtggaag actattgaaa taagtgtgga ttgtttcttt
    24481 gtgtttcaat cgtttcttgc cattcttcat ttaattttgg attaccgttt tcttccttga
    24541 actcataagg tcctaattca aaaatcccag acagtatttg attcacgatt tcatatagtt
    24601 cagtgtgatc agacacatga ttcttgaaga tcatctcatt atcaccacgt ttgaacctca
    24661 cattcgggct ctcatgatga acctctatgc atttttctcc tttttttaac tgacggactt
    24721 ctgaaaacat ctcataaata tcttctccag taaaaccttc agcaagcgcc agactgtcca
    24781 caagaccgcc tactgtcagt tcagagatta ttgcttgatt ggaatctaat tttaatgtat
    24841 agcttgtcgc ttctactacg ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc
    24901 taatcaattg aacttctttc attttctctt cctcctctta attttctact tatgtatttt
    24961 tatcacctga cataggtgac tcctatcaac gctatcatgt gcttttttcc tcagaaatcc
    25021 tatttatcca ttccatctat cccttattct tctacttttc tttctctagc atataagaga
    25081 tacgcatagt attcaagcaa ggattgtcga tataaaaaga acaaatactg ctattttgat
    25141 ttcttttggc tatattacat ataaataaaa tttatcatac ctgctctttt tgactatatc
    25201 atcatctgac ttctatactt cgtttgtatt aaaacttagg aggaatcatt catgcttaca
    25261 gggttaaact tattaaataa tccttttcta aataaaggaa cagcttttac aaaagaagaa
    25321 cgggcaaaat atggaatcac tggtatgctt ccaagtactg tccaaacact ggaacaacaa
    25381 tctgttcaag cttatggtca atacttaagc aaacaaacag atttggaaaa acgtatcttc
    25441 ttaatgaatc tattcaatac gaatcggaca ttgttttata aattgatggg cgaacattta
    25501 gttgaattca tgcctgtcgt ttatgatcct gtcgttgcgg attcaatcga acaatacaac
    25561 gaaattttct tgaaaccgca agatgctgca tttctttcaa tcgacgaacc agaaaacatc
    25621 aaagcaagtt tgaagaacgc ggcagatgga cgagacattc gtttgattgt tgtgactgat
    25681 gcagaaggaa tcttaggtat gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc
    25741 aaattgatgg tctatactgc tgctgctggt attaatccag cacaagtttt gcctgtatcg
    25801 atcgacgctg gtactaataa tgaagaatta ttgaataatc ccttatactt aggcaatcgt
    25861 catgctcgtg tagaaggcga aacttactat gaattcatcg atcaattcgt tcaatcagct
    25921 accgaattat tcccagaatt gttattgcat tgggaagatt ttggacgagg aaatgctgca
    25981 gcgatcttag aaaaatacga agatcaaatc actactttca acgacgatat ccaaggtaca
    26041 ggaattgttg tcctagcagg tgtattaggc ggattgaata tatccggtga acaattgaaa
    26101 gatcaaataa ttttgacatt tggtgctggt actgctggtg tgggtatcgc aaacatcctt
    26161 ttagatgaga tgatccgtca aggtgtacct gaagaagaag cacgccaaca tttctatcaa
    26221 gtagataaac aaggtttatt gtttgaagat acagaaggat tgactcctgg tcaaatccca
    26281 ttcgctcgca aacgctcaga tttcactaat gctgatgaat tgacaaactt agaagcagtt
    26341 gtaaaagcga tccatccgac aatcatgatt ggtacttcta cccaacctgg agcattcact
    26401 gaagaaatcg tgaaagaaat ggctgctcat acaccacgtc cgatcatcat gcctttgtct
    26461 aatccaacaa aattggcaga agcaaaagca aaagatttaa tagaatggac ggacggaaaa
    26521 gcattagttg gaacaggtat ccctgctgct gatgttgaat ataatggtgt aacttaccaa
    26581 atcggtcaag caaataatgc tttgatgtac ccaggtttag gacttggttt gattgcttca
    26641 acagctacac gagtaaatgc tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc
    26701 gttgatgtaa caaaaccagg agcagctatc ttgcctccag tagcaaaaat cactgaattt
    26761 tctcaaacga ttgctgaaac tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa
    26821 gaaatcacgg atatcaaaga agcagttgaa tctgctaaat gggttcctga atataaatct
    26881 ttagaagatt aagatcgtta tagtaaaaga acatacagaa actttgtatg acgtctaaaa
    26941 agaaaaaagc gactgcggca aaacagcggc cagccaactc ctgctctttt gtcgcttttc
    27001 tttggctttt ttcatatgat acagaataag aaaggatctg taaaaatggc acaaaaaaca
    27061 acaactgcaa ctgcagttga ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc
    27121 ggcgtaagtt tgccacttta tttgatcatg gtccttgtcc tgatcattgc aatggcttta
    27181 gggaaattac cgacgaatat gattggacca atcgcagttt tggttatttt cggtaattta
    27241 tttcatttta tcgggacaaa aatccctatc gtcaaaagtt atttgggtgg tggttccgtt
    27301 tttgcgatct tcgcttctgc tgcgattgct acatttggga tcttacctga atatgtagtg
    27361 aaatccacag aaaactttgt cagcaatatg ggctttttgg acttttatat tgctgccttg
    27421 attactggta gcatccttgg tatgaaccgt aatctattga tgaaagcttc tgttcgtttt
    27481 attcccgttg cacttatttc aatggtcgtc tccttttttg ccgtaggact cgtgggcatg
    27541 cttatcggaa atggttttgc aaattccgtt ttatacatct cattacctgc gatggctggt
    27601 ggtgttggag ccggagccgt tcctttatca acgatttatg caaatgtttt aggtacggat
    27661 gcttcttcta tcatttcccg cctgattcct gcgacagcaa tgacaaatgt cttagctatc
    27721 atcggtgcag cattagttgc tcgtgctgga caatcaatgc caaaattcaa cggcaatggt
    27781 aaattaatgg aaaaaggcga tcttggcgat ggaaaacctc gaactgtgaa accttccatt
    27841 ccacagttag gtgttggttt gatggtatca ttgactttct tcatcttagg acaaattggt
    27901 aactattttg ttcctaaagt ccatgcttac gcatttatga ttattatcgt tgtcatctgt
    27961 aaaatcgcca acctattacc agagtattat gaagatgcag ctatcatgtt caataacttg
    28021 attgtcaaaa acttgacagc agctgttctt gccggtatcg gtattgcgtt gctgaatctg
    28081 aatgtattag cttctgcttt gacatggcaa tttgttgtct tatgtctgac aagtgttatc
    28141 gcgatttcag ttgtttcagg atttgtcggt cgtttgtttg gtttatatcc aattgaatca
    28201 actattactg ctggcttatg caacaactcg atgggcggaa caggaaatgt agccgtctta
    28261 tctgcagcta atcggatgga acttattgct tttgctcaaa tgggcaatcg acttggtggt
    28321 gccatcgttt tgatcatctc tggtttcttg atgcaattac tttcttagtc aaaaaaacaa
    28381 aaatacctga tcatcaagta tcggcttggt gatcgggtat ttttttgata aagatttaca
    28441 ggattttcaa aaatcttttt tgcattttat cggaatgata accgatgtgt tcataaaatt
    28501 ggtgtgcttc tactctgtat tctgcagaat taagccttat tgtcgttatt cctcgttttt
    28561 tcgcttcttc ttctacgcca gtcatcaaag cctttccgat gcctcttttt tcagcatatt
    28621 tagacacagc aagtgccata atattgaaca ttggatcgct aaaaatcgat tcataggact
    28681 ccgcttgtac atagccagct gcttttttag aagtaggatc ttcataaacc aagaaaaaat
    28741 ttttctctgg ctccttcagc agtttcttca tttgtctcac cgtaacggtt aatggtatat
    28801 cataccctaa ttgatccgca ttgatttcac gtagtgcttc tgcatcttct aaacgcattt
    28861 cccgtaacat ccaaatcacc agccaatttc tgatttttta aaatgaattt ttttatcata
    28921 gtatactctt tttttcattt agaacaatat gtttcagtga ttttgacttg cgttagtcta
    28981 gattctccta atgtttgtaa gatgacaaag gcattgtaac aattgaccaa aagaaccacg
    29041 aggacaatta cttgaaaaat aaaggtcact ttttttggag acaaaaccct gctaaatctt
    29101 gctccccaca aacctccaat aacagcagcg gggataacat aaaacaacat agataaatca
    29161 aagcgcataa agctacttgt taaagcaatc gttaccaatt ttgccagttg agaaaagaaa
    29221 atcgtagata gagaatatag tgttgcttct tttattggca atgcaaacat cagcatcaac
    29281 aaagaaacat tgactggtcc accaccaata cctaaaaaac tagctaaaaa gcccagaacc
    29341 cccccacata taaggtacca agtccatgac gtcaaatgaa attttggctg atgatgcttg
    29401 gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa aaatttggat cagctgcaca
    29461 tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata caatgttgcc gactactcct
    29521 ccaacaacag ctccactaga tacccaaccg acaatctgcc aattcagtga tttcccgctt
    29581 gatacttgtc gcacagtcga aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa
    29641 gaaatccctg caacagaatg ggcgccgata agatcaagaa tcggtttgat caacacgcct
    29701 ccacccatcc cggaaatagc gccaactgtg ttcgccagaa cgatcaccac aaaataaatc
    29761 agaccaatca tcttgtattt cctttctctt gtttatatct tttagcgatt gttactcttt
    29821 tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa agcttttcta ctttttaagg
    29881 aaatttagtg ctaaacgaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    29941 cagccaaaca aaaatagctc ctcataattt tcgtttggtt gaacttatta ccgcagatgc
    30001 aagcttttga acaccgttta agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca
    30061 aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg
    30121 agttggcagg agaacaccgt ttaaggaaga gactgtgaag tcaacgtttt gacctgatta
    30181 ttagagaaac aaatggcaaa atagttcttc atatttcgcc gtttttcgag cgaatgccga
    30241 ggggcagctg attgaacatc gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac
    30301 aatccctttt tttatatctt attaattcat tcttttgttc tattcttctt cttccgccat
    30361 gaatgagaaa cccatatcaa agaactataa aggttatcaa aacagcgttt atgactaaat
    30421 gaattagagt tatctatcac tatttttcaa gcgatttaaa ccaaatggca tacaatatgg
    30481 catacaaaaa cttcaataag ccgtgggggt ttttaaattt cttcttgttc tttaaaatca
    30541 atgagtggag ctacataatt tgaatccact ttttcaaacg tcactttttg catcgacgaa
    30601 tgttttcttt cccaagataa cgtaaataga tttgactctg gacttataaa gtctacttca
    30661 ccttctagta ttactttctt atgcaaatat tcctctaata aatatagact tgctaaagca
    30721 tacaacacat tttccaaatt tgcttcctta taatttgaag aacgaccatg ttttactctg
    30781 ttatatattt tccaccaatc tggtgtatct tcaggccacc catctccaaa aggttttagt
    30841 gaagtcaatg ttaaagaatt cataacccta acaccattat ccaaacacat taattcgcta
    30901 gatttaataa cagctttaaa atcagttatg cttttattgc tactgctatc aaatccgcaa
    30961 agttctttga aaataatctc acactccgaa ccaatcgcta gtagtagttt ttgatattca
    31021 atagagaatg ttttgaaatt attctcatcg agcatcacaa aatctgtggt attaataaac
    31081 tcattttcta aatgtagata atacttccaa taaaaatttg taaactctgc tttgtccatt
    31141 aaatcacctc ttttgaatta taacaaaaaa aacacgtcca caacagacgt gttctttgat
    31201 ttctacacaa tcagcttgcc aaaaccatcc aatcagtcct agcatgcctg tattaaaatt
    31261 gaacgttcat catctatcat ctttcttagt ccagcatgtt tgcaaccaca cctacaattt
    31321 tcagtccaac aatagatttc gactagctaa gtcttgcaaa attcaggcag ctaacccttc
    31381 atttactacc ttaattatac taaataccca caacgtttac aagtgtaatc attctaagaa
    31441 atctgtttag ctatgatcgt tgtataatct ttaaacgccg ttccttcatt gattgaaata
    31501 attttataac tcttgttttt ccaagctact ttcatatcgt ttgttagttc ataatcttga
    31561 tcgtatcgaa taataaacgt caacgttcct tcaagaatgg tacctacact agcaagcacg
    31621 tcttttagca tttgtgaacg cacattagcc caacattcaa atacagtttc ttcactatcc
    31681 accatctgcc catcttcatc ttttacccgt tttgtctttt taaacttgac tctctcagtc
    31741 aattcattta cattcttaat gattggcata ataagcacct ctcatttgtt gtactaatgc
    31801 ttgtaccgtt actggtatcc gttcgctgct ggcttcttga cggttcaaat accaatgttg
    31861 agtaagtaat gacaccgcca aatcaaattg ctgataatta tccatatctt tatccgttgc
    31921 gctactatca atagcacttt ttatatattc gcctgctgta tcaatcaatt gttgaataaa
    31981 gtcatcatca atagtgtgat cgatccgcat actttttttt atgctatcca aaattaccat
    32041 tttatctctc ctaatgaaaa aaaggcagtg ggataaaccg ccgcccaata tttttctata
    32101 cttttggtgt tacaactggc gtaaactcaa tatacactga agcattttga tcaattttct
    32161 tataatcgtt gcgaacgatc actgcaagtc cttgtgagta gtaatcgaat ttttcccatt
    32221 gagtcgttac ttgatttcta cgagcaacaa agatagattg tgcaatatcc cccataatca
    32281 tagggaatgt tccagcttta ggattggcaa acaatgtatc tgcaatcaat actactggca
    32341 taccgaataa tgatttacca ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat
    32401 ctgaatcttt caatgtatct aggtggtaat atccgctttg gtttagaatt accattttat
    32461 ttaatgctgg gtctaatgcc acattgtata gttgtttcaa atcgtccaac gtagcagccg
    32521 ttttcttagt gaatgttttt aataaatcca tgatatgctt attgtcagtg ttttctacca
    32581 atttcgctaa ctgatctttg acctcttgta caatattcac tgctgaatct tccacaacct
    32641 catttgataa ggcaatctta ccagcacgag tttctacttt ataatcaact gaagtaaaca
    32701 tttcagcgtc aatatcacca atttcagcaa gttcagcttt tgttgctaac actgcttgtt
    32761 ggttagttgc tactggatat ttaccttgtc catttgatac tgtttttact gttgcatatt
    32821 gagctaagtt ataatttgaa cgtttcaaat caaagacttc accgattact tcttcgggaa
    32881 caactgccgc tgcatttaca gtagtgactc catcacgcac ttcgccttgt gaacggatat
    32941 attcttcata cccacgcaat tctgtttttt cgttatcgat taaagttttt tccatgttat
    33001 tactctcctt ttgtgcttta gaagtggatt ctaagctatt gtttgtttgt tttgcttggt
    33061 tgttactcat aaacgattcg taagaacgct tatttacttg gacatttgat gaatcataag
    33121 cgggaacggt cacgacacta atttcattaa gtgctttgat tttatctatt gatcgttcaa
    33181 ttgtgccatc ttcttttttg tcgaatgaat ctaatccaag cacgaaccca aaggacatag
    33241 aatcaacaac cccttttgag atattctcat acacatcatt tgaataggtc gtgtcggtta
    33301 gctctgcttc aaaatgtaat cctgtatcat cgacattcaa tttcaacgta cctgctttga
    33361 cactagctag tggctttgaa taatcgtgtc catataataa gaacacattt gataaatcca
    33421 cctcttttaa tgcttcgggt gtaatcactt ctacaaagcc gcctaaatct tttgacggtt
    33481 gcccgaattt caacgcatag cctgaaatag ttttgccgtc tgttggttgt ttttcatttt
    33541 ctttctctac tttgggagaa tcggctgtta gctcagcttc ttctgtcagc cgtttttcct
    33601 tttcttcttc attattcaac tggtgtaact ccttcctgtt cttttaattg atatttctcc
    33661 aacgtatcta aatacgtaaa gttcaaactt gctagtaaac gatcgccacc atccatgggg
    33721 ggtagcccca ttttcgaccg cccctcatta atcgtgagaa gtgacccttt gacctgatcc
    33781 aatacatttt ttacttttgt ttcaggatca gtctctagta actgatcgga attaaagcgg
    33841 atattggttt caagtttcgt atctaattca ctcacaaaca cattaaagta atgaatcagc
    33901 gtactttgga tatactggag attactttga acggtgctag aatgttcatt ttcaacgcct
    33961 aaacgctcaa tcggtacacc aaacgccttg gcaatttgtt ttgtgttcca atcattggaa
    34021 ttgataagct tcaaaacatc ggtgtttatt tctaatgttt tgtagtccat tgtttcatca
    34081 agaatgatcg ttctaagagc attatctcca ctacttgaac cgttggcttc ttcaaacttt
    34141 tcacgaattg cgttctttgc tgatccgtct aaatctgact tatgaacctt caaaatacct
    34201 gacccactga caccacgagt aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt
    34261 ttcagttcat cacgtaacgc ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa
    34321 tatttgaagt gcaaaatatc gctagacttc acacgtcttt ttttatcacc aatctgataa
    34381 gacaacgtac cattatctaa ttgagtaacc gttacacttg agttaggtaa taagtgaatt
    34441 tcttctacct tttcaccgtt gcgtttaatc tctgcaaagc tattaccgtt tagcaacata
    34501 ttgaccgcca aagcaaactt taaatgccaa ccatccattt ctgaattagg tttctcgttt
    34561 agtaacttca ccagttcatt atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga
    34621 cttgaagcaa tatcactagc aataattcga acggctgcaa acacatcact atttcttaat
    34681 gcacgaacgc ttgtaaagct ggtggtgtaa tcatccgatt ggattgatac cacatgttgt
    34741 aggaaaggat ctcccgttgt attttgtctc gctttaaaaa atgccatctt ttaacctccc
    34801 ttctgttctg tatttagcat gaccgctacc ccaataagca ctatgcccag cgctaacaag
    34861 ccatagtaga catttgttag taaggtgata gcaaccaaca cacacaccaa tccaagaagt
    34921 aaaagaacgg tatgaatgta attagaaaga aaattcttca cttgtataaa attcattgtc
    34981 tgctttcgct ccttccgttg tggtgaagta atccattgca aacacatagg cattgattaa
    35041 tgcagctatt ggatcaattt tgttactgtt ctttgattta ttaatctgaa tgccgttgtt
    35101 atccgttttt aaaatagcgt tgttcactgc atgggtgaga atcgtatttt tattgtgaat
    35161 aacgttgcct tcataaactt gttctctaaa agttcgggtc ggaacgttta gagtaatcgt
    35221 tccttgtctt acttctaaca ttgggtagtt ggctttttca gctttactga taagcgaatt
    35281 agcgttatac gggtcataac aaatccccat acattccaaa tcattttctt gaatcaaatc
    35341 ttgaataaac tgaaatactt ggtcatagtc cacgattcca ctttctaatt gagtaatgga
    35401 acactcacca gctttttcta gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg
    35461 aagcccatat ttggtggcta caaagctatg tgaatcacaa taaagttcgc cgttatctag
    35521 cggtacgatc catgaaacgc tagttaaatc atctgtttta gataaatcca ctccgatata
    35581 aacgggcttg cctgtaatat cttgtggttc tacttcggtt gcctgccagt cttcagcaat
    35641 catatagctg tcctcactcg cttgtctcca taaattgaag tttttcacca atacagaatt
    35701 aaggttattt tgtttcaaag caaggttcac atcatcttga atggttggaa tcattacttt
    35761 tttgatttct tcactttcaa aaatcggatt ggctttgatc catttctctt gatcgtgaat
    35821 ttcttcggga tcgtccaatt cccatatcgc tataaagtac ctgtcggctt gttcttcgcc
    35881 ttttagcaca cgatctaaca ataagtattc ctcgtacata ggaacgttta aatcaagccc
    35941 agcggtacta ataacacaca ataagccgtt cttttgttgg gtcattcctg acttaatgac
    36001 gttgtacgtt ttacgagttt ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc
    36061 aaacccatct agcgtgctgg tttcactagc cagtgccata gcgaacgaat tagagggcaa
    36121 gtgctgaatc ttagagttca taattttcag ttgtggtctt aaaaacttac tagacttgac
    36181 cacgtttcta agtgaattag acagcatatc atagcccaat ttagcttgtt tcaaagcgtt
    36241 acttacaaac aatacttgcc ttgcttctgc tggttcttgt tccataatca acgcattggc
    36301 agccatgcca cttgcgatat acgttttccc attcttacgg ctcatactgg taaacgctcg
    36361 attgaatcgc ctgtaattcc cttctttagt acgccagcca taaagactgc ctagaatgaa
    36421 tttttgaaat cctaacatct tgattgcttt accgtccgta gatggtagca actcaatgaa
    36481 tttaatcgct ttggtggctt gttcttcatc gaacacataa gggaagctat ctgacttaga
    36541 acgctccaaa tcgcttatat gacgctcaca cgcttggata attttcttgg gtgcaatcag
    36601 cttacctgat aggactttct caatgtaatc attcatgaat tcatcaactc tttgaacggg
    36661 tcttcgggtt catcattttt aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc
    36721 atacctagtg aggtggcttg ttttttcaaa tcactcatag cttgtgattg aacccgaacc
    36781 gctggattgg ctttctttac tccgttttcg catgtgataa acgtacctga ttttcttatc
    36841 tcggcttcgg ctgttttcat tcgagcaaag gctaaacaat aggctaccaa tgaaccataa
    36901 tctgcttcac tcaatggata atcttttttt aatgttggta cgatacgatt ccattcactt
    36961 ctagcgctcg ctgccatcca gtcagggggc gtgatattta ttaatggttg ctgcttgaac
    37021 aattcttctc tagcgtccac acggattgct ttttcttcac ttgatatatt gccttttgta
    37081 tcgtgtaata ttttagggtg tcccatgttg tcctcctttc agacaaaaga aaaaggaacg
    37141 taagcaatta agctcacatt ccttcgtttt tcgatcagaa tttttcttta tctaatttat
    37201 ctatataaat gatttttccg ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact
    37261 caatggtgcg ctgtttccct gttttatcag taataacaat tgtatttttc aataaatctc
    37321 ctactttcat tatatacttt ttcacttgtg attacaagtg taatcgttat ttgtaattac
    37381 ccttatgcct tttcacaacg gttatttgta ccaaaatgag taggcatcga ttgtcagtaa
    37441 gtttcacact atgccccctt aactcaaagg gtactctttt ctcgtctttg tctttcattg
    37501 gctgtcttgg aattatggtg tgcatggcat aaagaacgaa aattagtttc atctaaccgt
    37561 ctattccaat catcttttag ttcaattttg tgatctacaa catcagcttt ccttatcaag
    37621 ccttcttcta ggcaatcctc acatactgga tgatttaacc gatacaagta tgaagccttt
    37681 cgccatgttc tactttgata gaattgaaaa tatttacctt cgctttgctt tctttcttca
    37741 cgcttaattg ccgtttgctt tgctttatgc ttcccacaat acttctgcct ataatcaatc
    37801 aatgttttac atccagcatg attacattgt tttttaactg ccatagatta ccaaccaaag
    37861 cgatctcgtt cgtcagaatc atcttgttcc cattttgatt tgtattcaat ataaggcgaa
    37921 actatcttca agcacgttcc gtctttaaaa ttaactttag tgattttatc ttcctcaaaa
    37981 ctaataaagt ctacatcttc ttgagtgttt gaatactcaa tgtgtttctc accatcgaag
    38041 tatctaatac tttccatatg cataattatt ctacctccaa taaattagtt atcaattcat
    38101 catctgttag tagcaaagcc aagaacttat catggatgtt taaaaacaaa ttatcctcat
    38161 tcgatgtaag ctctcgttgt ctcgctcgat catataggtt aagcagacaa ttaaattcat
    38221 ccattagctc acctgtatca tcatagttgc cactatctac caatgatccc actactagcg
    38281 attgacacca cgctactgac tcctgcagcc aatcaggttc ttcttccatt atctttaata
    38341 cttctggatc aaatgtggaa taatcttctg ttaccaatcc cacactaaat gccagcgtgt
    38401 tcattagaaa ccgatccatt tcatctgtat attgaaagtt cggtgctttg tttgttgaaa
    38461 taatattgag catttacttt cctccttgat tcgtaacacg tggtaacacc tacgtaacag
    38521 gtacccgtta ccactcaatc ccaacgggga caagggttct agcgttttgg taacaggtaa
    38581 cgggtaaaat ccagtaacat tattccccag catattagct aactatttca aaagttttaa
    38641 ggaaataagg tgttaccccg ttaccaaatt gctgtatccc ttggggcagt aagggtaaac
    38701 tagtaacggg ctaggtgtta ctgacccgtt actacccgtt actttttgta ctttccagct
    38761 cgatttttat tttcataggc atttgactta ttgtaaaatc ctcgtcgttt atttcttttt
    38821 ggcagttgct tccgttccca accaggaata ctgctaataa tcaaggcaat ctttttagtt
    38881 tctgcatctg atcgaccatt caataaatcc tttgcttgtt tatcaaaaac agcttccaaa
    38941 atatcagcgg tgagtacgct atcaactaaa taaaactctt gttgcatagt agctccatta
    39001 tagaaatgct gtaaattttt ctcattacat ttactgtcag gatagttctg ataataagaa
    39061 cgtctcgccc acgctggagc ttcttcccat ttatacggga tttccatttc caaatattta
    39121 gtgattgctt ctttaattgg attctccatc ttggcatcct cttgatagtc ttttgcaatt
    39181 tctaagactt ctttatcttc atcgggacta aaatagattc gttgtccttt atcaaaaagc
    39241 acctttgctt cagctaacac ttgaagaaaa tattcatcct ttgttttaaa cggattcatc
    39301 tttggcttat tcttacaagg caaaggataa aaacggcggt ttcctgtatc atctttcaaa
    39361 tactgcccat cattcgacgt tccaataaat acacattgcc ttgcccaagc tatgacattc
    39421 cgaccataag gaggtctaat tttatcgatc ttggcactga tgaagttttt tatctgttca
    39481 attttagttt tgttcattga agataattct ccaagttcaa taattacatt gccttgaagc
    39541 tgcatataat catctttctt ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc
    39601 gtgtaaagta atgctggaac ggtactctta ccaatccctt gcggtccatc caaaactgga
    39661 acgatctcaa actttattcc tggtttatag atacgtgcga ctgatccgac tatccaacgt
    39721 tttgttactt ctcgtgtgta aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt
    39781 tccagtctag gctgttgatc ccatttaact ttttcgatac gttcttttac tgggttgaaa
    39841 acattctttc tagccacatt tactactgct ttgtgtattt tgttgtcatc aaaaactaca
    39901 ttgtagtttt cttcaatata tgccgttaag gcatcctcaa aactgtcttc ccattctcca
    39961 gcggtacgat tgatccaagg agaatctttt aataggtgaa tgtatccgct aaattcgtta
    40021 taagcaattg ttccagctaa attgctatca ttggttaaaa tcaattctgc attcacaaca
    40081 ctgttacttt taatctgatc gtctttattg catcttaatc gtgacatcca ggttttcaat
    40141 tcaatgatat tgtcttcacg ctcgctggct tcgggaaagt ctaaaatact atctaattta
    40201 tgcttatgag ccatgagctt tccccctcaa atctttttta atcatcgatc gaataatttg
    40261 attcacttct ttatctttta gcggtggttc attgtacgca tttgctgtat aaatcaattc
    40321 tgctaaagtt tgattatcta caccgtgcca tagtaaccaa ccgcataact tagttaaatg
    40381 atcgtttcta ctaccttcgc cagcgccttg aacaatcata tctaattttt ttgctgtccc
    40441 tgtttttgct acgggtcggt attgagtctt tatttcttgc ggttttttta gcagtaggtt
    40501 cactagccaa tcgggagctg gtgcaatatt atttagagat acgttttcaa taggttcata
    40561 tgctctccca acacttcttg aaataatcat agttggcgct attggaactc ctttagtgat
    40621 aatatcaatc ccgcttaaac ttttaccttt atcatcacgc ccgaagatat cagcaaatgc
    40681 acctgtttct tgttggataa ttacaccatc aggaatttta aagaagaaat ggattccttt
    40741 acctggtgtg atttccgtat aagtagaagg aaatggatca tacattgaag caagtttttt
    40801 ataattctca acgccgtttc gtgtttcaga atgttgatct aggtcaacta ccaccatttg
    40861 atgatcttca agcattaacc caatatttct agttgcatct tccgtccacc attcattaat
    40921 ttgattagaa tcgttagttg catcattcca accactagag ccttttattg gaattttagt
    40981 tccaggagat aagggataaa ttgaccatcc cgacttttgt aattccgttg ctgcttgata
    41041 attatttgcc aataaacctc tcctcctttt tcaaaatact ggtataaatc attcagacac
    41101 ctccatgagc ttcaactgct caagttcgta tttcaaccca tcccgaacaa attcagcacc
    41161 ttttaattga ttctgatact ttacccatgt attaaatgtt ttcacccttg cttcttgcat
    41221 atcagtaatg tttaaccatg cggttaaaat ctgatttttt agctcgttga tttctcgaca
    41281 aattattttg tgttctgcat gttttttgag tacatacaaa actgttagtt tttcattgaa
    41341 tgaatttcca aagtaggttt tcccgatatt attttctagt tcagcgatcc cttgattaat
    41401 ttcctctaat tcaaggctat cgagttggtt taatagtttt gttttgtaca tctacttatg
    41461 ccctccagtt cttaattgat agtcgtactc gtcataagca gtgaagagat ataacgctaa
    41521 tcctgcaaag atagctattt ttgcaattaa tggtaataag ccgaaaattc cgccgattaa
    41581 aatgcttcct ataattaagt ttcgtttcat aatggttccc tccgattttt taatatggta
    41641 taatcggaac aaagaatatc gggttttatc ccaattaagt tgcctttatc ctgtccgcca
    41701 agactgttat ggataaggct ttttctttgt tctcgatcat gttaatgctc ctaagttgtc
    41761 tgcaattcag cagaacgatt ataaattttc tcactcactg aaaactgcaa cccatatttt
    41821 tctttaacag tcattaattc tactgtctca tctaaaatag gttcacgatc aaccaacatt
    41881 tcaggagtca tatctttctt ttttaccatt ttggaatgac cataaatagt tgatacagct
    41941 ttattggcta tcacattcgc tttaatgtag tctactggtt ttggttcagt aatagcgtgg
    42001 cttaatttag tcattgcttc tttttggtgt tctttgtcta gcatgcggaa tacttgaaag
    42061 ccttcaagtc ctgtggactg gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat
    42121 gcctcggctt cttttttacg gcttttgaat gccatgcggt aaatattttt tttcgttgat
    42181 gatgtttaca tcttgtgttc tgcccaatga atcgatgacc ttactagtag taaccccatc
    42241 tttaggcaaa gagtgaatgg ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc
    42301 tttcgccact gcccaccatt catcattaat atcgacaaat cttataatgt gtccgttcca
    42361 aatttcagtt ttcattcaac gtcctccagt tcaagcccta gcaagctcgc taacaactca
    42421 acttgctgat acaaaatttc ttgatactct tcaatagaaa gtcgttcttt aaactctgct
    42481 tgtactaaat cgttcaagcc acgcaatact ccgcttaccg ttggttcttg tggtagcaat
    42541 ttttgatagt ccatttgttc gtacaaacat tcaaacgttt cttctagcgc tttgccttcc
    42601 aagtttgcta attctgccgc tgtgtcttct gtaagaattg ttgataatac tttttcgatt
    42661 gctttatttt gtgtcgattc cattattatt tcctcctatt aatccctttg cccccacggc
    42721 ttgcgtttat ttttggtatt cttccataaa gcgatcacat tcatcttgtg aaattcttac
    42781 gactccatca actataatca actttaaacc gttaggaaca tatttctgca tcatagtatt
    42841 gtatgaaacg ttcatatact tagcagcttg tgccttgttc atatattttg gaaactggta
    42901 ctttttttct aataatgaaa gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg
    42961 atcgtcaata attttaaaag ctggatcatc aagcgtgagt ttgtcaattt ttaattccat
    43021 atttatcccc cctcgttagc atacatttct atacaaagat tttaaaaaaa tgcaagaggc
    43081 tcttgaatac atttctatac ctcttataaa ctcaatatac catcatacct tttcatttgc
    43141 aagaatttta acatactttt ttgaactttt atgtttcaca catatttaaa tcgtgatata
    43201 gtagagaaaa tatatgaatg gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt
    43261 ggagacagga caaagaatta gactggctag aattgaaaag ggactctcaa tggaacaact
    43321 tggaggtaaa ttatccccac cggccagtaa aggtgccgtt agtaattggg aaaatggcta
    43381 taatcttccc aataacacca ggcttaaaca actttcaaag gtattagatg tatccactac
    43441 ctatctatta aacggatctt atacattaag agatatgcat atgatgcccg aaattaagcg
    43501 aaatgtgctt gaaaagcaat tgaaaaaaac tattgaaaaa aataactcta taattgattc
    43561 taatctttct aatgctatca ataacatcga agaagataac ctgactgtct cacagaagat
    43621 ttatttaaca aacgcaataa aatatcttaa aacaggatct cgaattgaat ttcctgacgg
    43681 gagtacccca attgcttatc tcgcagctct tctaaatacg cttttacgat atgactcatt
    43741 aattaatgat tcagaaaaaa atacagatga gaaaaaagag tatcaagaat tttatgaaaa
    43801 tgatcttcta aaagagttca cagctcttgt tactgagcta aataaacact attcaaaata
    43861 ataataaact gcctcctcca accgccccca cggcttgcta cactatggag ggaaaactat
    43921 tatggcaaca ttcaagcaat atgaaactaa aaaaggtaaa ttttggctct atgaagccta
    43981 tcttggaatg aacaaaatga ctggcaaacc tgataaggtt cgtagacgag gttacaaaac
    44041 aaaaaaagaa gcccagcttg ccttgtcacg cttgcaagtg gactacgata aaaacggtat
    44101 taaaaaagcg aacaacgaaa cgtttaaaga agtttatgac ttgtggctag aaacatataa
    44161 aaccactgtc cgagaagcca ccttcatgaa aactgaaatc aagtttagaa agtggattct
    44221 tccaaaatat ggaaatcttc gtgtgaatga agtcacggta aaaaaagccc aacaaattgt
    44281 aaatagctgg gctgaaacta ctgaccaata caaggtactt cattcaactt ctaagcgtat
    44341 ttttagctac gctatcaatc ttgggattat tgagactaat ccactagaca acgtattaat
    44401 gcctaaaaga accaccacta agcataaaga aaaggtcaaa gtttacagta aagaacaact
    44461 gatcaccttg tttgattacc taaacagcaa acctgaaact tatcgaaacg actttgataa
    44521 aacgttacta cgctttctct tttatagcgg ggttaggatc agtgaagccc ttgcattgaa
    44581 ttggtcagac attgattttc aagaaaatac ggtcaccatc aataaaacac taagccaatc
    44641 caaaaatggc tataaaatca gtgatcctaa aactgattgt agtgctgggg ttcttccact
    44701 agatgaaaga acaatttcta tattaaaaaa atggcagatt aatcagcgga aatatatgct
    44761 gacacttggt gtaactgatc cgactatgat tttttgtggt atctataaag aactaatcac
    44821 acaccacgct atttatgcaa gattgattac tatttgtgaa aaagctatgg ttccatttct
    44881 cggtgtgcat gtagcaagac acactcatgc ttcaatgctc ttggacgctg gagctactat
    44941 ggtagaagtt caaactagat tacggcatac aaaaattagt cagacgatcg attgttacgg
    45001 tcacttggca aaagaaacga aagaaaagac tgttgaaaaa ttagtacaac acttaaatat
    45061 tgactagatg gcatacaata tggcatacag taaaaaataa aaaaaggaga aaccctatca
    45121 tatcaaggtt tctcctagct ttatagtcta ttcttcttct tccgccatga agttaagctc
    45181 attaaagata attgacgata gttggtattt caacgatttt tgaatagaac taatcacttt
    45241 attaagtcta attcacctta agtggttttt tagtcgtatt caaaaacgta ttcaagttgc
    45301 gtggcgtgct tttgataaga aatcgtaaga actagttaat caaacgactt ataaataatg
    45361 taacaccact gattaatcag ctatatccaa aaaaacaagc attcattcag atacatcaat
    45421 ctctatagaa tacaaagctt tcattatctc aaaccattac cacaacttgg caagtcggtt
    45481 aattgaagaa cacctcattc tccacttata gcagtggagc aacaagttga gctattgttg
    45541 caacagaagc taagaattct gtactccatt taagtttttt tagactatct accgcaaacc
    45601 tcagcatagt ttttttaggc ttttctttaa tagcttcatc tttaatggtt tcgatatact
    45661 cctgagcctc ttctacctct tcttcaggta aggaatcatt caaattctcc aaaagcaaat
    45721 caagctttga taataattcc gacatatcat caatagaaac aatcatatta ttattgtggc
    45781 cttgctgtat attagtgttg taactatttc caaatatatt tactttatta tatattgaat
    45841 tgttgctcat atagtcctcc agtcgtcctg ctgcattact tgcatcttgt aacgtcacaa
    45901 aaaaatctct cccatgttgg taaattgatt caacgatttc aaattctgga tttccatatt
    45961 cgcgccattc gtattcttcc ttaaagtatt ttccgtaact atggttaaat tgcacagaat
    46021 ttttaccaat tttccttaga agttttatct ctctctcaga aataaaattt tcctcaaaag
    46081 ctgattcaac tatctgaaaa tcactaagaa atgttcctaa acagaagatt gattcattaa
    46141 tagtttgttg aaaattccat ggtattttgt tccaaaaacc attcttaatt aaagcctctt
    46201 ttttttcaac aatctgtgat tttacatttt ctgtatatat cttccaatta ttgacgtctt
    46261 ccttaatgtc cgaaatcgat tgaccttcat aagaagtccc tccaccaaaa agtccttgaa
    46321 ccattttatc tcatcctctc tataataatt actctaaatc caatacttgc tacaaatgat
    46381 agcatttcgt gtggtaacat gattgccatc gttcctgttt tatttactct gacttcaata
    46441 cttggtttgt tgatgaaatt ttaccaattg attccatcga tcgtttcatg gaatctgtat
    46501 ttactatttt cattgattgt agagattgat ttattccctc tgttgcactt ctgatacctt
    46561 tagtatcaaa gttttgcatg attgctcgct gagctgataa agctgataat gtttgaccta
    46621 ttgactctat cgacctcttc atggaatctg tatttaatat ttttattgat tgtagagatt
    46681 gatttattcc ctctgttgcc cttctgatac cttcagtatc aaagttctgc ataatttctt
    46741 gctggactga taatgtttga cctattgact ccatcgactt tttcatggaa tctgtattta
    46801 atactttcat tgctgataga gactctgaca atttagtaac acctgctaaa tcgatattac
    46861 taaaataatt tccaatatat ccttcaaaat catctgacat atcactaagg tcctcaccag
    46921 caatatctcc agccaatatt ttttgaaaag ctttctctag ttgatttgta tatctcttca
    46981 atttctttat taatttttgg tataaagcca tttctatttc tttgttatgg cttacaatat
    47041 tcctaccttc tctgattaaa ctcatgtctg tttgaatttc ttctagctta acatccatat
    47101 atttactaaa tacttcactc cacaaagaat atgaatcaga tatagtgtga ctgttatctt
    47161 gaaataactt caataattga tcctgagata tttctttata ccttacaata ttctcttcat
    47221 tttcttgtgt aactaaaata taattttctc caaagagata ttcttcaaaa tcatataaat
    47281 ctatccattg taaaagattc tttgagtcaa actttcctat tgcttgtatt tgttttcttt
    47341 ttattgacga aatgatttca tttggaattg attcctctgc ccagttatca gaaaaatatg
    47401 ccggagctaa taaatagatt aattttctta atgcccattc gtaactatgc aaatacggta
    47461 atagtttttt cgaaaaaaac aacgcatttg tatctaaaag ggtagtgtag tttaattttg
    47521 atttacttgc ttcaatattg accttagcta ggaaaatttg aaatatatgt acctctttta
    47581 ttcctatctt accagtctta ttatattgaa atactacgtg taacgaatca ttatagttct
    47641 ttatgtcaaa gccaaattta taattttttt cctgctcctg aaatcttaag atagaatcac
    47701 taatatatgg tgacaacact ttcagaaagg tttcttcgat attttccatt cttccttttt
    47761 taggataata taaatattca acaacttgct tactaatatg tcatcaactt ctttcctttc
    47821 aacaatttta attttcctat taacttgata tttgctacat aagccacaac tagcaaacag
    47881 aaagagaact ttttctttac cttgataact gtgttaatta ctggtttctt ttttctattt
    47941 ctttatagta ttgttcttct acttttaata tttttttaac ttctcttttt atgtcagcat
    48001 acatagatat actgttgtcc tttcggttga agcagtgata attctcacca tcaaactcat
    48061 aaaaaagcat atcacttgag cttccatctg aattaattaa ctgaaatttg gtattgagta
    48121 gtaattcatt tttctcgtca ttcaacttta ttaaaatagg tttgatctct tttaaataat
    48181 tgaaatatat ttcggttatt actaaattaa atttcgctac tgtataaaca taagagatac
    48241 gtggggaatc tctaaattgg ttatatatat ctttccttaa aacatctaac tcctttttta
    48301 attgtccgtt aaaatcaaaa tgaggcattg ctagaatttc atccaaatca aagtacactc
    48361 tttgttgatg aatatttact ggcagatgac catgctgaga ataatttctc atatgtaata
    48421 aaaacctata cgaaaaatgc tcatcataag gcttactcag aattttattc ttaaaagatt
    48481 taaaatcttt ttctgctaaa aagtttttca tacaaacttc aatggattca gtcaaagttt
    48541 tggctgaact aattaagttt atcgtcaaag catttatctg ataataatac tggtcttccg
    48601 ttaatttttc acctgtactt tcaattaaat cattagtaaa aattgtgaag tg
//
LOCUS       DAZKAO010000005        48575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000005 DAZKAO010000000
VERSION     DAZKAO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.005"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8H8_000228"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8H8_000228"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFP8985336.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8H8_000229"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8H8_000229"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFP8985337.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8H8_000230"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8H8_000230"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFP8985338.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8H8_000231"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8H8_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985339.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACG8H8_000232"
     CDS             complement(5146..5298)
                     /locus_tag="ACG8H8_000232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985340.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACG8H8_000233"
     CDS             complement(5315..6634)
                     /locus_tag="ACG8H8_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP8985341.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7012..7104)
                     /locus_tag="ACG8H8_000234"
     CDS             complement(7012..7104)
                     /locus_tag="ACG8H8_000234"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985342.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            complement(7375..8355)
                     /locus_tag="ACG8H8_000235"
     CDS             complement(7375..8355)
                     /locus_tag="ACG8H8_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP8985343.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="ACG8H8_000236"
     CDS             complement(8368..9246)
                     /locus_tag="ACG8H8_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFP8985344.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="ACG8H8_000237"
     CDS             complement(9262..10062)
                     /locus_tag="ACG8H8_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP8985345.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10337..10486)
                     /locus_tag="ACG8H8_000238"
     CDS             complement(10337..10486)
                     /locus_tag="ACG8H8_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985346.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11454..11780
                     /locus_tag="ACG8H8_000239"
     CDS             11454..11780
                     /locus_tag="ACG8H8_000239"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985347.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11788..12696
                     /locus_tag="ACG8H8_000240"
     CDS             11788..12696
                     /locus_tag="ACG8H8_000240"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8985348.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13322..13576
                     /locus_tag="ACG8H8_000241"
     CDS             13322..13576
                     /locus_tag="ACG8H8_000241"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFP8985349.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="ACG8H8_000242"
     CDS             14118..14276
                     /locus_tag="ACG8H8_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985350.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="ACG8H8_000243"
     CDS             14348..15574
                     /locus_tag="ACG8H8_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8985351.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="ACG8H8_000244"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="ACG8H8_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP8985352.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16499)
                     /gene="rplM"
                     /locus_tag="ACG8H8_000245"
     CDS             complement(16071..16499)
                     /gene="rplM"
                     /locus_tag="ACG8H8_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP8985353.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17033..17500)
                     /locus_tag="ACG8H8_000246"
     CDS             complement(17033..17500)
                     /locus_tag="ACG8H8_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP8985354.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17620..19158)
                     /locus_tag="ACG8H8_000247"
     CDS             complement(17620..19158)
                     /locus_tag="ACG8H8_000247"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP8985355.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19238..19924
                     /locus_tag="ACG8H8_000248"
     CDS             19238..19924
                     /locus_tag="ACG8H8_000248"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP8985356.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="ACG8H8_000249"
     CDS             complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="ACG8H8_000249"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8985357.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20736..21412)
                     /gene="pgmB"
                     /locus_tag="ACG8H8_000250"
                     /pseudo
     CDS             complement(20736..21412)
                     /gene="pgmB"
                     /locus_tag="ACG8H8_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            complement(21409..22537)
                     /locus_tag="ACG8H8_000251"
                     /pseudo
     CDS             complement(21409..22537)
                     /locus_tag="ACG8H8_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(22537..24720)
                     /locus_tag="ACG8H8_000252"
     CDS             complement(22537..24720)
                     /locus_tag="ACG8H8_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP8985358.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24717..25742)
                     /locus_tag="ACG8H8_000253"
     CDS             complement(24717..25742)
                     /locus_tag="ACG8H8_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8985359.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25787..26614)
                     /locus_tag="ACG8H8_000254"
     CDS             complement(25787..26614)
                     /locus_tag="ACG8H8_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8985360.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26611..27678)
                     /locus_tag="ACG8H8_000255"
     CDS             complement(26611..27678)
                     /locus_tag="ACG8H8_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP8985361.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27704..28540)
                     /locus_tag="ACG8H8_000256"
     CDS             complement(27704..28540)
                     /locus_tag="ACG8H8_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985362.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28537..29406)
                     /locus_tag="ACG8H8_000257"
     CDS             complement(28537..29406)
                     /locus_tag="ACG8H8_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985363.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29476..30759)
                     /locus_tag="ACG8H8_000258"
     CDS             complement(29476..30759)
                     /locus_tag="ACG8H8_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8985364.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30773..32458)
                     /locus_tag="ACG8H8_000259"
     CDS             complement(30773..32458)
                     /locus_tag="ACG8H8_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP8985365.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32733..33758
                     /locus_tag="ACG8H8_000260"
     CDS             32733..33758
                     /locus_tag="ACG8H8_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985366.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(34353..38006)
                     /gene="rpoC"
                     /locus_tag="ACG8H8_000261"
     CDS             complement(34353..38006)
                     /gene="rpoC"
                     /locus_tag="ACG8H8_000261"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP8985367.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(38085..41702)
                     /gene="rpoB"
                     /locus_tag="ACG8H8_000262"
     CDS             complement(38085..41702)
                     /gene="rpoB"
                     /locus_tag="ACG8H8_000262"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP8985368.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            42430..43398
                     /locus_tag="ACG8H8_000263"
     CDS             42430..43398
                     /locus_tag="ACG8H8_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8985369.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            43416..44321
                     /locus_tag="ACG8H8_000264"
     CDS             43416..44321
                     /locus_tag="ACG8H8_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985370.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            44318..45124
                     /locus_tag="ACG8H8_000265"
     CDS             44318..45124
                     /locus_tag="ACG8H8_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985371.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            45281..46249
                     /locus_tag="ACG8H8_000266"
     CDS             45281..46249
                     /locus_tag="ACG8H8_000266"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP8985372.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(46566..47090)
                     /locus_tag="ACG8H8_000267"
     CDS             complement(46566..47090)
                     /locus_tag="ACG8H8_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP8985373.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            47377..>48575
                     /gene="scm"
                     /locus_tag="ACG8H8_000268"
     CDS             47377..>48575
                     /gene="scm"
                     /locus_tag="ACG8H8_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP8985374.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDST"
BASE COUNT        13845 a        10450 c         8848 g        15432 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtaa ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    16921 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    16981 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    17041 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    17101 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    17161 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    17221 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    17281 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    17341 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    17401 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    17461 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    17521 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    17581 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    17641 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    17701 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    17761 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    17821 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    17881 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    17941 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    18001 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    18061 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    18121 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    18181 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    18241 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    18301 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    18361 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    18421 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    18481 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    18541 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    18601 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    18661 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    18721 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    18781 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    18841 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    18901 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    18961 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    19021 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    19081 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    19141 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    19201 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    19261 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    19321 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    19381 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    19441 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    19501 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    19561 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    19621 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    19681 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    19741 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    19801 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    19861 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    19921 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    19981 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    20041 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    20101 tccataaata gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    20161 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    20221 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    20281 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    20341 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    20401 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    20461 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    20521 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    20581 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    20641 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    20701 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    20761 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    20821 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    20881 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    20941 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgcgtcaa
    21001 taaggacatc aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc
    21061 ttgctgatcc caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg
    21121 caccaggcaa aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt
    21181 tttgctcagc aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg
    21241 acagcaatcg ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct
    21301 cagtgaatgg aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag
    21361 ctgtgtctac aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc
    21421 tccttgaaaa tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt
    21481 atcagtggct tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac
    21541 ggccgaaatg ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc
    21601 tatcacagga acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag
    21661 actttgtcca tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgtat
    21721 cgaactccag taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg
    21781 ctaacagtcc tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag
    21841 gggcaaatct catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt
    21901 tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag
    21961 taacatcagt aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat
    22021 caactgtagc aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat
    22081 cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc
    22141 cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc
    22201 ctactcctcg cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc
    22261 cgacgatttc tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt
    22321 tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg
    22381 ctaatgatga aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct
    22441 gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac
    22501 tttctttgaa agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat
    22561 cagttctttt tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt
    22621 ctcttttttt attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca
    22681 aataaaagga aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt
    22741 tccattcgtt actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa
    22801 tgaagcagca tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca
    22861 cgcttcttgg aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc
    22921 gtgaatcgct ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt
    22981 cttagaaacg attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc
    23041 tgcttgcttg ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga
    23101 ctggctgcca ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt
    23161 gtcatcttgc ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa
    23221 attttgacat tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac
    23281 attataatgc gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc
    23341 tggaccaatg actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca
    23401 aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc
    23461 tgttgcttgg acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc
    23521 ggcagctgaa gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg
    23581 tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata
    23641 atctcttgct ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt
    23701 tgctacttca ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa
    23761 aacatgccct ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc
    23821 attgcgggga gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa
    23881 agaagaatcg ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat
    23941 cgactgctct aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt
    24001 aaacgggtct gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga
    24061 cgtatttttt ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa
    24121 tacacctgct ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg
    24181 gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg
    24241 tgtaccaaaa ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc
    24301 ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac
    24361 acgacaagta atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt
    24421 aagctctccc atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag
    24481 agaaaaatgt tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa
    24541 ctcggctgat tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac
    24601 ttgattccga tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt
    24661 catctgactc gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat
    24721 aaggtaataa cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg
    24781 acatatcctt gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca
    24841 aaatgagcca tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc
    24901 agctttccat ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg
    24961 ccacaaaaag ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt
    25021 tggatgatct tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat
    25081 ttgcttggat cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag
    25141 ctgtacgccc gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc
    25201 atgtgaatgc cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt
    25261 ccccagcctg gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg
    25321 agaacatcaa gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca
    25381 taagccaaaa tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct
    25441 gtcatagctg gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca
    25501 tgttcgaagt gaaagcgatt ggctgtacag acactcacga tatcgggaga aatctcattc
    25561 agcatttgtg taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct
    25621 gctcgctggc tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga
    25681 taataagcag catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc
    25741 atttatttct ctctcctttg cttatttact tattgttatt ctttctctat tctttctcta
    25801 aatcatttaa atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat
    25861 aaattttcac ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact
    25921 cattttgaag tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt
    25981 agcgatgatt atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt
    26041 ttgaaatatc atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt
    26101 tcaattgatt agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt
    26161 ggtatcggtt caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta
    26221 aataacgaag tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca
    26281 tcggcggtaa tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc
    26341 caacctcgct tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt
    26401 cttcgataaa gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc
    26461 ctgtttcttt gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga
    26521 tttcgtattc ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg
    26581 ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc
    26641 cattttgata ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc
    26701 tgcaaatggc acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa
    26761 ttcccaacat gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc
    26821 actagatgct cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt
    26881 aaacccagct ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat
    26941 ccctcttaaa gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac
    27001 tccacgttta tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag
    27061 cgtgtaatcg gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc
    27121 aacgatggta gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc
    27181 taaaccgaag attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa
    27241 agcgaattga gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt
    27301 acgtagttta taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt
    27361 gcagactaac tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc
    27421 gacaatcata tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc
    27481 tcttggcaag aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc
    27541 tctaaagtcg acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac
    27601 ttcgttcttt tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa
    27661 tgcaactaat tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc
    27721 cttcgactaa atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta
    27781 aagcggtcac caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata
    27841 atgtgttcaa accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa
    27901 ataaataatc attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa
    27961 tagccaatgg tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg
    28021 ctgcttcttc aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag
    28081 taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta
    28141 gaccggaaat gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg
    28201 tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg
    28261 ccccgctgat cccaatcact actgcagtta cggaggctat caaggagatc actaaactat
    28321 ttttgaaata catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa
    28381 aagtccattc tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga
    28441 atgatgtcat cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg
    28501 tcgcgtaaaa taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg
    28561 tgagagcaag acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt
    28621 tccaagatca ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt
    28681 attcgtcagt agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat
    28741 cacagttgct agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc
    28801 atttgcaccg tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc
    28861 caaaaatgaa ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa
    28921 gaagctagca actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc
    28981 tactaacaga tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat
    29041 caaagaaata gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa
    29101 tctgaacttg cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac
    29161 aactaagaat gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa
    29221 aatttcaaga taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat
    29281 atcgaaaaaa ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa
    29341 aagcatgctt ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg
    29401 attcattgaa acctccccct gataatctgc caatagctat ctactttttt cgaacaccat
    29461 tggcagaact tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc
    29521 ttcgtttccg acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc
    29581 tcccattttc gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc
    29641 actagcaatc tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc
    29701 atccagttcg actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca
    29761 cttttccatg tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg
    29821 tttctcgtct agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc
    29881 tttttgtgtt gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg
    29941 caaaatgtaa gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat
    30001 ttctgtcaca tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa
    30061 tgcttctctc atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc
    30121 tccatttgac aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg
    30181 catagcaatt ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat
    30241 atcatcccaa ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca
    30301 aataccttga acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg
    30361 aacaaggttt ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg
    30421 gactgccgtc tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac
    30481 ttccattact tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc
    30541 tgattcttct accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc
    30601 aaccagttca ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat
    30661 cgttgtctgt tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc
    30721 agtagctagc agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta
    30781 ttattttcag ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat
    30841 ggataatttg tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg
    30901 attgattgga aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac
    30961 tgataaaaga atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt
    31021 ctttttttcg aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat
    31081 ctgtctgcaa ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat
    31141 tctctcctgt tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat
    31201 aaattgccgg cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc
    31261 ttctccattc cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct
    31321 catatggact ggtcgttcca tctgggtttt tcttgtagtt tactcgggca ccctctttct
    31381 tcagctgttc cactaattca aggatcgttt tttccggcag aatacctcgt accggattca
    31441 atccgatacc gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat
    31501 cgatggtttt taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg
    31561 tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat
    31621 aactgatatt atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg
    31681 cttcatcagc gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac
    31741 aagaagttcc tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc
    31801 ctttttctaa gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa
    31861 aattcaagtc tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt
    31921 tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg
    31981 tatccagtgg caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct
    32041 gaaaccaagt actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt
    32101 cgcttctcat ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat
    32161 ttacaacgga gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc
    32221 taaatgtatc tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct
    32281 gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca
    32341 gtttgcctgc ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg
    32401 cggttttctc agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg
    32461 gattcccctt tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt
    32521 actgtcaata gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct
    32581 tatgtatcaa taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta
    32641 ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct
    32701 aaaacgctcc caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga
    32761 aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct
    32821 ctgataaagc aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca
    32881 aaattgcaag aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta
    32941 cttctcatac gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg
    33001 tttctgaaca aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc
    33061 tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct
    33121 ctacgatgga tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa
    33181 tcggaaaagt cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg
    33241 atagttacga tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc
    33301 atgctccgac agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat
    33361 ttgatcacca cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag
    33421 atagcatggc agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt
    33481 ctaccgatgc attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga
    33541 tacctgatga tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc
    33601 cacagttgac tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt
    33661 tattccaact aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga
    33721 ttactctcac agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga
    33781 aacactgagg ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc
    33841 ctcccggtat tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac
    33901 tataatgttt gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt
    33961 tgtgtataag tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt
    34021 tatttgggga tacttatctc taaaacataa atagaataac agacatgtct actggttttg
    34081 ttttattcga ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct
    34141 tcccatcaat ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc
    34201 tcttggaaga tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct
    34261 ttcccacttt tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac
    34321 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
    34381 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
    34441 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
    34501 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
    34561 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
    34621 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
    34681 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
    34741 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
    34801 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
    34861 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
    34921 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
    34981 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
    35041 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
    35101 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
    35161 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
    35221 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
    35281 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
    35341 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
    35401 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
    35461 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
    35521 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
    35581 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
    35641 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
    35701 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
    35761 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
    35821 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
    35881 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
    35941 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
    36001 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
    36061 ttgtacccta agtctttcat acggtcaagc atcttagaag tttcttttac tttgaatcgt
    36121 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
    36181 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
    36241 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
    36301 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
    36361 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
    36421 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
    36481 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta
    36541 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
    36601 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
    36661 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
    36721 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
    36781 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
    36841 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
    36901 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
    36961 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
    37021 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
    37081 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
    37141 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
    37201 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
    37261 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
    37321 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
    37381 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
    37441 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
    37501 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
    37561 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
    37621 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
    37681 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
    37741 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
    37801 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
    37861 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
    37921 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
    37981 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
    38041 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
    38101 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
    38161 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
    38221 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
    38281 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
    38341 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
    38401 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
    38461 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
    38521 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
    38581 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
    38641 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
    38701 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
    38761 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
    38821 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
    38881 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
    38941 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
    39001 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
    39061 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
    39121 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
    39181 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
    39241 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
    39301 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
    39361 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
    39421 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
    39481 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
    39541 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
    39601 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accatcatgt
    39661 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
    39721 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
    39781 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
    39841 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
    39901 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
    39961 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
    40021 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
    40081 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
    40141 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
    40201 tcacgagtca atccgccagg tcctaaggcg aaaagacgac gtttgtgtgt caattcgcca
    40261 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
    40321 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
    40381 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
    40441 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
    40501 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
    40561 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
    40621 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
    40681 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
    40741 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
    40801 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
    40861 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
    40921 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
    40981 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
    41041 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
    41101 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
    41161 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
    41221 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
    41281 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
    41341 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
    41401 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
    41461 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
    41521 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
    41581 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
    41641 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
    41701 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
    41761 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
    41821 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
    41881 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
    41941 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
    42001 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
    42061 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
    42121 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
    42181 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
    42241 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
    42301 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
    42361 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
    42421 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
    42481 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
    42541 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
    42601 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
    42661 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
    42721 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
    42781 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
    42841 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
    42901 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
    42961 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
    43021 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
    43081 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
    43141 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
    43201 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc
    43261 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
    43321 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
    43381 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
    43441 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
    43501 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
    43561 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
    43621 tagggagtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
    43681 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
    43741 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
    43801 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
    43861 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
    43921 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
    43981 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
    44041 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
    44101 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
    44161 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
    44221 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
    44281 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    44341 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    44401 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    44461 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    44521 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    44581 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    44641 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    44701 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    44761 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    44821 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    44881 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    44941 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    45001 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    45061 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    45121 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    45181 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    45241 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    45301 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    45361 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    45421 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    45481 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgatgacttc
    45541 tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt
    45601 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    45661 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    45721 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    45781 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    45841 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    45901 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    45961 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    46021 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    46081 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    46141 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    46201 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    46261 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    46321 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    46381 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    46441 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    46501 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    46561 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    46621 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    46681 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    46741 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    46801 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    46861 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    46921 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    46981 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    47041 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    47101 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    47161 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    47221 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    47281 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    47341 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    47401 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    47461 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    47521 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    47581 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    47641 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    47701 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    47761 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    47821 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    47881 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    47941 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    48001 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    48061 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    48121 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    48181 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    48241 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    48301 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    48361 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    48421 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt
    48481 gatagcacgg atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtgat
    48541 agcacggaca catcagcttc aagtgacagt acaga
//
LOCUS       DAZKAO010000006        42320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000006 DAZKAO010000000
VERSION     DAZKAO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.006"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..1603)
                     /locus_tag="ACG8H8_000269"
     CDS             complement(35..1603)
                     /locus_tag="ACG8H8_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP8985375.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1607..2017)
                     /locus_tag="ACG8H8_000270"
     CDS             complement(1607..2017)
                     /locus_tag="ACG8H8_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP8985376.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2014..2937)
                     /locus_tag="ACG8H8_000271"
     CDS             complement(2014..2937)
                     /locus_tag="ACG8H8_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP8985377.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2934..3812)
                     /locus_tag="ACG8H8_000272"
     CDS             complement(2934..3812)
                     /locus_tag="ACG8H8_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP8985378.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3799..5136)
                     /locus_tag="ACG8H8_000273"
     CDS             complement(3799..5136)
                     /locus_tag="ACG8H8_000273"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP8985379.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5139..6242)
                     /locus_tag="ACG8H8_000274"
     CDS             complement(5139..6242)
                     /locus_tag="ACG8H8_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8985380.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6316..7824)
                     /locus_tag="ACG8H8_000275"
     CDS             complement(6316..7824)
                     /locus_tag="ACG8H8_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8985381.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7790..8482)
                     /locus_tag="ACG8H8_000276"
     CDS             complement(7790..8482)
                     /locus_tag="ACG8H8_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP8985382.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8504..9478)
                     /locus_tag="ACG8H8_000277"
     CDS             complement(8504..9478)
                     /locus_tag="ACG8H8_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985383.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9778..10650
                     /locus_tag="ACG8H8_000278"
     CDS             9778..10650
                     /locus_tag="ACG8H8_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985384.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11108..13306)
                     /locus_tag="ACG8H8_000279"
     CDS             complement(11108..13306)
                     /locus_tag="ACG8H8_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985385.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13538..14530
                     /locus_tag="ACG8H8_000280"
     CDS             13538..14530
                     /locus_tag="ACG8H8_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985386.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14590..16011
                     /locus_tag="ACG8H8_000281"
     CDS             14590..16011
                     /locus_tag="ACG8H8_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP8985387.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16004..17503
                     /locus_tag="ACG8H8_000282"
     CDS             16004..17503
                     /locus_tag="ACG8H8_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP8985388.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17542..18936
                     /gene="uxaC"
                     /locus_tag="ACG8H8_000283"
                     /pseudo
     CDS             17542..18936
                     /gene="uxaC"
                     /locus_tag="ACG8H8_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            19095..19778
                     /locus_tag="ACG8H8_000284"
     CDS             19095..19778
                     /locus_tag="ACG8H8_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP8985389.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19780..20616
                     /locus_tag="ACG8H8_000285"
     CDS             19780..20616
                     /locus_tag="ACG8H8_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8985390.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20627..22381
                     /locus_tag="ACG8H8_000286"
     CDS             20627..22381
                     /locus_tag="ACG8H8_000286"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985391.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22657..22998)
                     /locus_tag="ACG8H8_000287"
     CDS             complement(22657..22998)
                     /locus_tag="ACG8H8_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8985392.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23066..25237)
                     /locus_tag="ACG8H8_000288"
     CDS             complement(23066..25237)
                     /locus_tag="ACG8H8_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP8985393.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25448..26305
                     /locus_tag="ACG8H8_000289"
     CDS             25448..26305
                     /locus_tag="ACG8H8_000289"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8985394.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26376..27233)
                     /locus_tag="ACG8H8_000290"
     CDS             complement(26376..27233)
                     /locus_tag="ACG8H8_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8985395.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27218..29920)
                     /locus_tag="ACG8H8_000291"
     CDS             complement(27218..29920)
                     /locus_tag="ACG8H8_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP8985396.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29933..31222)
                     /locus_tag="ACG8H8_000292"
     CDS             complement(29933..31222)
                     /locus_tag="ACG8H8_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP8985397.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31372..32418
                     /locus_tag="ACG8H8_000293"
     CDS             31372..32418
                     /locus_tag="ACG8H8_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8985398.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32420..34573
                     /locus_tag="ACG8H8_000294"
     CDS             32420..34573
                     /locus_tag="ACG8H8_000294"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP8985399.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35046..36497)
                     /locus_tag="ACG8H8_000295"
     CDS             complement(35046..36497)
                     /locus_tag="ACG8H8_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8985400.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36494..38218)
                     /locus_tag="ACG8H8_000296"
     CDS             complement(36494..38218)
                     /locus_tag="ACG8H8_000296"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8985401.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38211..38825)
                     /locus_tag="ACG8H8_000297"
     CDS             complement(38211..38825)
                     /locus_tag="ACG8H8_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8985402.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39170..40627)
                     /locus_tag="ACG8H8_000298"
     CDS             complement(39170..40627)
                     /locus_tag="ACG8H8_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8985403.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40668..41588)
                     /locus_tag="ACG8H8_000299"
     CDS             complement(40668..41588)
                     /locus_tag="ACG8H8_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985404.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41600..>42320)
                     /locus_tag="ACG8H8_000300"
     CDS             complement(41600..>42320)
                     /locus_tag="ACG8H8_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985405.1"
                     /translation="SDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKV
                     YQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVF
                     IGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTIL
                     AVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVG
                     FVLLMITNTIARRFDSDSALF"
BASE COUNT        12413 a         8659 c         7528 g        13720 t
ORIGIN      
        1 taagttagtg aacctcattt attaaagaat taaattacag atgatctttt ccatcatact
       61 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
      121 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
      181 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
      241 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
      301 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
      361 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
      421 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
      481 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
      541 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
      601 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
      661 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
      721 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
      781 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
      841 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
      901 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
      961 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     1021 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     1081 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     1141 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     1201 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     1261 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     1321 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atttgtggcg
     1381 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     1441 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     1501 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     1561 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     1621 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     1681 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     1741 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     1801 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     1861 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     1921 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     1981 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     2041 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     2101 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     2161 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     2221 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     2281 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     2341 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     2401 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     2461 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     2521 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     2581 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     2641 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     2701 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     2761 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     2821 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     2881 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     2941 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     3001 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     3061 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     3121 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     3181 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     3241 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     3301 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     3361 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     3421 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     3481 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     3541 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     3601 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     3661 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     3721 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     3781 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     3841 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     3901 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     3961 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     4021 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     4081 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     4141 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     4201 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     4261 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     4321 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     4381 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     4441 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     4501 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     4561 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     4621 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     4681 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     4741 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     4801 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     4861 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     4921 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     4981 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     5041 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     5101 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     5161 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     5221 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     5281 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     5341 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     5401 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     5461 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     5521 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     5581 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     5641 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     5701 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     5761 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     5821 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     5881 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     5941 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     6001 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     6061 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     6121 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     6181 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     6241 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     6301 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     6361 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     6421 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     6481 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     6541 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     6601 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
     6661 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
     6721 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
     6781 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
     6841 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
     6901 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
     6961 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
     7021 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
     7081 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
     7141 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
     7201 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
     7261 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
     7321 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
     7381 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
     7441 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
     7501 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
     7561 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
     7621 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
     7681 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
     7741 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
     7801 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
     7861 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
     7921 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
     7981 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
     8041 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
     8101 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
     8161 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
     8221 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
     8281 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
     8341 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
     8401 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
     8461 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
     8521 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
     8581 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
     8641 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
     8701 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
     8761 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
     8821 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
     8881 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
     8941 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
     9001 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
     9061 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
     9121 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
     9181 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
     9241 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
     9301 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
     9361 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
     9421 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
     9481 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
     9541 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
     9601 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
     9661 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
     9721 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
     9781 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
     9841 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
     9901 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
     9961 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    10021 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    10081 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    10141 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    10201 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    10261 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    10321 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    10381 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    10441 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    10501 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    10561 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    10621 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    10681 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    10741 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    10801 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    10861 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    10921 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    10981 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    11041 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    11101 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    11161 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    11221 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    11281 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    11341 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    11401 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    11461 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    11521 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    11581 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    11641 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    11701 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    11761 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    11821 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    11881 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    11941 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    12001 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    12061 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    12121 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    12181 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    12241 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    12301 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    12361 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    12421 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    12481 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    12541 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    12601 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    12661 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    12721 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    12781 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    12841 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    12901 cttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    12961 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    13021 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    13081 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    13141 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    13201 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    13261 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    13321 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    13381 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    13441 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    13501 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc
    13561 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    13621 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    13681 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    13741 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    13801 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    13861 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    13921 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    13981 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    14041 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    14101 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    14161 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    14221 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    14281 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    14341 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    14401 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    14461 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    14521 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    14581 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    14641 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    14701 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    14761 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    14821 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    14881 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    14941 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    15001 ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    15061 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    15121 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    15181 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    15241 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    15301 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    15361 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    15421 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    15481 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    15541 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    15601 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    15661 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    15721 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    15781 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    15841 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    15901 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    15961 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    16021 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    16081 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    16141 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    16201 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    16261 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    16321 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    16381 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    16441 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    16501 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    16561 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    16621 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    16681 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    16741 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    16801 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    16861 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    16921 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    16981 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    17041 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    17101 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    17161 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    17221 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    17281 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    17341 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    17401 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    17461 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    17521 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    17581 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    17641 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    17701 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    17761 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    17821 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    17881 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    17941 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    18001 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    18061 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    18121 aatgcatgag tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    18181 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    18241 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    18301 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    18361 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    18421 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    18481 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    18541 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    18601 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    18661 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    18721 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    18781 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    18841 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    18901 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    18961 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    19021 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    19081 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    19141 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    19201 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    19261 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    19321 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    19381 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    19441 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    19501 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    19561 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    19621 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    19681 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    19741 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    19801 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    19861 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    19921 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    19981 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    20041 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    20101 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    20161 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    20221 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    20281 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    20341 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    20401 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    20461 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    20521 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    20581 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    20641 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    20701 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    20761 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    20821 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    20881 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    20941 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    21001 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    21061 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    21121 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    21181 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    21241 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    21301 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    21361 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    21421 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    21481 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    21541 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    21601 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    21661 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    21721 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    21781 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    21841 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    21901 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    21961 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    22021 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    22081 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    22141 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    22201 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    22261 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    22321 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    22381 acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga ataaacggtg
    22441 ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
    22501 ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg
    22561 tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg
    22621 tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac
    22681 tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg
    22741 catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat
    22801 tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat
    22861 cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc
    22921 ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct
    22981 atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac
    23041 atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag
    23101 ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt
    23161 cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag
    23221 aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat
    23281 ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga
    23341 agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt
    23401 cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat
    23461 ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac
    23521 ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca
    23581 tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga
    23641 tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat
    23701 cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc
    23761 caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg
    23821 ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca
    23881 aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat
    23941 gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt
    24001 ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct
    24061 gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta
    24121 caccacctgt attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag
    24181 catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct
    24241 gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat
    24301 actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat
    24361 ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt
    24421 cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat
    24481 cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg
    24541 ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg
    24601 aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca
    24661 agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt
    24721 ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg
    24781 tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa
    24841 tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg
    24901 catcttggat atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca
    24961 gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt
    25021 ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga
    25081 cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct
    25141 ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag
    25201 cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg
    25261 attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt
    25321 caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa
    25381 gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag
    25441 tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga
    25501 cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa
    25561 aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca
    25621 gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc
    25681 gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg
    25741 aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc
    25801 ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag
    25861 cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga
    25921 acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat
    25981 gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga
    26041 taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac
    26101 acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca
    26161 taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat
    26221 gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa
    26281 agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat
    26341 gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct
    26401 aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca
    26461 cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt
    26521 tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac
    26581 gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga
    26641 tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct
    26701 gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga
    26761 tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca
    26821 ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt
    26881 ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa
    26941 gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct
    27001 tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg
    27061 ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga
    27121 tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca
    27181 tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt
    27241 cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct
    27301 tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca
    27361 atttttctaa atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc
    27421 tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg
    27481 aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt
    27541 atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga
    27601 accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt
    27661 ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat
    27721 tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt
    27781 gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca
    27841 ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga
    27901 cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac
    27961 tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt
    28021 ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg
    28081 cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta
    28141 ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag
    28201 gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat
    28261 tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca
    28321 aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt
    28381 ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt
    28441 ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg
    28501 gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata gtggccccac
    28561 tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt
    28621 caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag
    28681 ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg
    28741 tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt
    28801 catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc
    28861 aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat
    28921 ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta
    28981 gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg
    29041 ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt
    29101 cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact
    29161 cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg
    29221 gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat
    29281 ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc
    29341 cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc
    29401 accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc
    29461 caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt
    29521 gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc
    29581 cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga
    29641 taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg
    29701 cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg
    29761 tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat
    29821 cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat
    29881 cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt
    29941 ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa
    30001 accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg
    30061 ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg
    30121 ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg
    30181 atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg
    30241 ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg
    30301 cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt
    30361 catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct
    30421 cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg
    30481 tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca
    30541 gatatccata acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa
    30601 aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc
    30661 gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt
    30721 ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa
    30781 gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga
    30841 tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga
    30901 aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga
    30961 tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag
    31021 tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat
    31081 tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc
    31141 agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt
    31201 ttggtatatc tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga
    31261 ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt
    31321 tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa
    31381 ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct
    31441 gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc
    31501 acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg
    31561 aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca
    31621 cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag
    31681 ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt
    31741 ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct
    31801 cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc
    31861 gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc
    31921 caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat
    31981 ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa
    32041 gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta
    32101 gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat
    32161 gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat
    32221 ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca
    32281 accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg
    32341 ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa
    32401 tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa
    32461 tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa
    32521 ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg
    32581 cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca
    32641 tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag
    32701 ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg
    32761 ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta
    32821 cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat
    32881 agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt
    32941 ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc
    33001 caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg
    33061 atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa
    33121 ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg
    33181 tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg
    33241 ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta
    33301 tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga
    33361 tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat
    33421 gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc
    33481 tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt
    33541 aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac
    33601 ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg
    33661 ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga
    33721 tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca
    33781 gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa
    33841 agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc
    33901 agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg
    33961 aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc
    34021 aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat
    34081 agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag
    34141 ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt
    34201 cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta
    34261 tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg
    34321 tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga
    34381 agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga
    34441 atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca
    34501 attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc
    34561 aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    34621 aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct
    34681 tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt attagagaga
    34741 caaatggcaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
    34801 gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga gtattaagaa
    34861 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
    34921 ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct gccttgcctc aagtaataag
    34981 aacagcccaa aattagattt ctagacaatt ttttataagt tatttcatta cattggcttt
    35041 ttcttttatg attctccact gttttctcgg tactctttag gggatctgcc gacgattttc
    35101 ttgaataatt tagagaaata cgtggtgttg ttgtagccaa tctcacttgc tatctcattg
    35161 atgttttgat tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa atggttcaaa
    35221 taagctgaaa aacttttttt cgtttctttt ttgaacagct gtcctagata catgacattt
    35281 aaatgcaggt tttctgacac ttctttgagt gtaagtggtt cttgataatg ctggtgcagg
    35341 attacgatta ctttttccac attgtcactg tactttttct gtctctgatg ctttttgatg
    35401 aatgacagaa gtagctgctg aagttcttgc accgtttttg cctgatggat tttttcgatt
    35461 ccttgtaaat attcctcatc ttccagatca atcaattccc gttgaatctc cataaacagt
    35521 aaaaaactgg aatatcgtat atcttccggc atcatggctg ctaactgaaa ctgttcaaaa
    35581 aaatcagtga tcattttttg agcgacttcc aattgtttat tttgcagcac gcgtctgaaa
    35641 cttgaaaaat cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa aacatgctcg
    35701 tctttatctc cataaaattg atgcaactgt agattgtctt tcgcttgttg gaatgtttca
    35761 gggattttgt ccaaagaatc tgtctcttcc ccgatactga tcagccattc ctgatcagga
    35821 aagttttctt gaacaaaaca acagaagcca tcaacttcat ctgcctccaa caacaacgtg
    35881 atcaatacac ggtctccgta atttcgttga tagtaaaaga tttgttgccg ttcttgcaac
    35941 catctattga taagagtatc atgagttctt gccagttgaa tcaatactat cctatgctgc
    36001 ttctcactta tttgtcctat tatttcttct tcacttattt catccaattc ttcattcaac
    36061 cattgactga ataacagttc ttgatagatt tcttgttgat ttttttgttc attttgctga
    36121 tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt cgaacttatt cacgggtttc
    36181 atcaaataat tcacggcgcc taattgcata cctcctttca aataatcgaa ttcttggtat
    36241 cctgataaaa tcataaattc aaaattatga cgttcttttt gagccgcttc aataaattcc
    36301 agtccattta tttctggcat tgtcacatcc gttactacta aatcaatctg tcgctgttca
    36361 agtacagcta gtccttcttt ggcattctct gctgttgcta ctatttgaaa gcctaaactt
    36421 tgccaatcaa ttatcttttt taacccattc aaaatcatgt attcatcatc taccaataac
    36481 acttgataca cgctcatccc tccttgaatg taatagaaac ggtcagtcca cctgtctcat
    36541 tattctcaag cgacatataa taactgctgc cgaagctggc acgtaaacgt tcattcacgt
    36601 tttgcagacc gatagaaccg tgaagttcca ctttaggatg agacagtttt tcttcaatct
    36661 gttttagccg tttttcagtc atcccttttc cattatcttt gatgatgatc cttacccgtt
    36721 tgccttcctg caagactttg acacttagcg cattatcgaa tcgagtaaaa tcaataccgt
    36781 gtttaaaata attttcaact aatggctgga tcacaaattt aggtacttcg atcttttcca
    36841 aatcaggatc gatcataaaa tgataggcta ctcgattagg ataacgcatt tgatacaaat
    36901 agacatattt ttcgcaaaag tccagttctt cttttaaagt gatcgttttc tcttgattcg
    36961 tattgtttcg aagcaaagcg gaaaacgcat acacaacatc tgctaattct tcacttcctt
    37021 cgctgattgc atacattcgg atatattcta atgtattata taaaaagtgt ggattgatct
    37081 gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg tagatgtctt
    37141 ctacaaactg attgatgcta tccaacattt cgttgatacc gattgaaagt tccttcagtt
    37201 catattccgt gtttgttatg tcgatccgcg tctctaaatt ccctgctgct gtttgatcca
    37261 tggcctgcat gacgatagat acttgctgtg aatatcgttt gaatgtttta taaagaaaga
    37321 taagcagtat attgtctaaa ataatgatac caatgatcag cggggcaaga ttttttcccg
    37381 tttcgatttt tacacttttt ttatctagta cagctaatat acgataatca tcagacagtt
    37441 cttggtgttg aacaaaattg tttttttcca gatcgtcaat cggcaattgt gaatttttct
    37501 tcagactctc tgtgatgatc atttcttgtc tttcaaacgt ttctttagct attttattat
    37561 catggaatgt gtatagtcga tttgtcgttc cagaaatcat gtataaagag agtccattga
    37621 atgacgttag attttctaaa atcatatcta gttctttctt ttgaaaacca ataaaaatac
    37681 ttcctgactc agctcctcct tgtgtgatag gcaacttgat ataaaaggcg tctagtaatt
    37741 ttggtgtccc cttttttatt tccccacctt tgttttctct agaagattca taatactccg
    37801 gtagttgatt caacacgata tatatcgcac ttagttcttc ataatttgcg tagagatctt
    37861 tgatttgatt tgggaaagaa actaattcat cggtactgtt ttgctgttcg tatacataca
    37921 tcagatactg atctatgggt tttgttaagt aggtcgtcac attttcgatc ttttcggaat
    37981 tccctacaag ctggtttttg atgatcctac tcaaggttgt tttatcattc agcattctgc
    38041 ttgtgctttg aacggcatca cttgtcatgt tttctgcctg tttttgattt tgttcccaag
    38101 tatggatact aaaaataacg gtagcagtca tcgttacacc gatcatgata accgcgtatt
    38161 tccgcaacag acgatttagt aaggttcctt ttgaaaatag aatccgaaac ttattcataa
    38221 ctttccaact gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc attccaaacc
    38281 gtcaaaagac caatgacacc aaacaggatc aatcctttga attgccaagt cacgcctaaa
    38341 attataccgc taccaataat tatttttaga aaagacgaaa agctggaaaa aaccgaaata
    38401 aaacttagct ttagcagatt aggaacagag atcgtatatt cactatctaa aatcatactg
    38461 tattgataag tagcaaatag gcagagcaag gcaaaaagta aaataaaatc aatagctaaa
    38521 aaaatgagcc ctttgatttg aacagataaa tataaattgt aactcaataa agcagtacca
    38581 gataaaaata aactaaatag cagattgcct cttaaaaaat ttcttttata tatattccag
    38641 cctcttttta ttgtgatttc tttatactcg aatccgtaca aataaaatag ttcattgact
    38701 gttttccatg ccgcacctaa tccaaagatg atcccgccgg caaatgtata tatccagaaa
    38761 tacaaggtta gttttataat gacccagatc cggataaata actcttccaa aaccttccct
    38821 aacatatctt ctcctccaaa cattaagttt atactttctc attatacaaa aaaaggttgg
    38881 gcaatcagca ccttgacctg attattagag aaacaaacgg aaaaatagtt cctcatattt
    38941 tgtcgttttg tgagcgaata ccgagggtta gctgattgaa caccgtttac caagagtttg
    39001 tgaaatagcc gcttagtttc gagtagtaag aaaaattttg tgaatatggc tttccacatt
    39061 tttacatata tttagcttaa taccgagaaa ctggcttttg aacaccgttt attcggaaca
    39121 agaggttgtg acacgtcttt tgtcacaacc cctttgtctt tattcattat tattcttgtc
    39181 ggaactcatc atattgtttt tgcatttctt tcaatacctt atcatagcca gccttgtcca
    39241 aagcgtcttt caatttcggt aatgtttcat ctggatcaac agtccctgta ttcaatccat
    39301 ctaaatattg attcatgaca ttagaaatat tacttaattc tgttttgaca ctgtcagtgt
    39361 tgaagctgaa tcctaaaatc ggtgaagttt ctgcatcagc aatcgattga tcacgtttgg
    39421 caatcatgtc atccgtaata gactcttgtg tatatagaat cttgttgttt cctgtattcc
    39481 aagcagacat atgtgtattt ggctgataac catctagcaa tttgatttta tcatctgaac
    39541 catcgacttt ttcccatgct tctccctcga tcccccaaac aagcccattc aataattctg
    39601 gatcactgtt aagcagccct agaagttcta ctgatttttc tttatttttt gaagtatttg
    39661 atacgacaaa atttgccatt tgtgcttgag ctgtagattt tagtggaaca gtaattgctt
    39721 ttgagacaag ttcttgtttt gctgcatttg tcaaaattgt atcgccataa tcatacggtc
    39781 cttgtgtttc tccacgcatt agccaagtat ttccttctag tggataatcc ttattgctcg
    39841 ttgcagcgtc acttggaatg agtccttgtt tgtaccagct atgcattgta cgtaacagtc
    39901 cgatatacgt ttcattatcg tattggttga tgattttcga atcatctcca tccagatcaa
    39961 tagcaaatgg taacgtattc cctaaaggat aatcaaagtt cccttggact ttgaagcctt
    40021 gtccaatggc aaaagctgcc gtgtttggtt ctttttcatg gaattccttc aagacttttt
    40081 ctgcatcagc gtaagattgg attccatcaa ttgaaagatc gtatttatct agtaaagctt
    40141 tgttaaaagt aagtacttgt tgagcaaaga catttccatt gactgggaaa gcatataatt
    40201 ttccattgac cagatttcct ttaatatatg cttcatctaa atcattataa gctttttcag
    40261 cgtacttagg cgctaattca gtcaaatcag caaaggctcc tttttgggca ttggaaacat
    40321 agttgttcgc aaaggcgata tcataatttt ccccggaaga aatgatgacg ttcattttct
    40381 tttcgtaatc gccccagcca atgtaattga tattgacttt tacaccgatc tctttttcga
    40441 ttcgtttgtt tgctacttcc atcaattgat cgaaattatc tggtttatct ccgatttgat
    40501 acatctgcaa agtgtccgaa ttgctagctt ctgcctggtc tttagaacca gaacctccgc
    40561 atgctgctaa tgttcccaac aacaacgctg aaacacctgt aaaggctagt ttcttccata
    40621 acttcatttt tcttcctcct aattttatga tttatatttt tgtttcttta ttcttttact
    40681 ccgccgattg ttagaccttt gacaaaatgc ttctggaaaa atggatagct gatcgcaata
    40741 ggcaacgtcg agatgactac gatcgccatt ctggcagatt ctcctggaac tgaggctaat
    40801 ccgcctgtca attggcttcc tgctcctgaa ttttgtgtca gatattgaat attactttgg
    40861 attttcatca aaagatactg tagtggaact agcttatcat cttggatgta tagtaaagca
    40921 ttgaaccagt cattccagta acccaatgcg gcaaataaac tgattgtcgc aatacctgga
    40981 acagctaacg gtaaaacgat ctgaacaaat attcgcagtt cgctagctcc atcgatccgt
    41041 gccgattcaa taatcgaatc agagaccgaa cgtttaaaga atgtgcgcat cacaataata
    41101 ttgaagggac tgacagccat tggcaagatc aatgcccaaa tcgtatcttt taactgaaga
    41161 agatttgtca taacaagata attggccacc atccctggtg agaacaacat cgtaatcaaa
    41221 caaaaaacgg taaaaaaacg acggaatgga aagtttgacc gtgaaatcgc ataagcatat
    41281 aaagaagtag ccgtgctatt gatcagtgta cctaaaacag taacaagtac agtgacgaaa
    41341 agcgcttgga aaattttatc ctgcatctgg tcgaacaagt acgtataacc aaatatactg
    41401 aacttttctg gccaaaaact gtacccgtga gtcgacaata tggactcatc ggtaaaagag
    41461 atggcgataa caaatataaa tggcaaaata caggaaatag caaagagtgc aatcattata
    41521 ttagagaaaa aattagccgt cttattgaaa gagcggactt ggactctctc cactttcttt
    41581 tttttcattt ttttcctcct tagaataatg cagaatcgct atcaaaacgt cgggcgatcg
    41641 tatttgtgat catcaacaag acaaagccga caactgattg gtaaagtcct gcagcagccg
    41701 tcataccaaa atctcctgtg gatgtcaggc cattgtagat atacgtatcc aaaacttgag
    41761 tcacatcata taatgcaccg gaattcctag gaatgttata aaacaggcca aaatcagctc
    41821 ggaaaatgtt tcctacggct aaaatcgtta aaatcgtcat tagtggaata agttgtggta
    41881 tcgtcacatg tttgatctgc tgccatttgc tagctccatc aaccatagca gcttcataat
    41941 aagtcggatc gattcccata acagatgcaa agtaaataat gctgttataa ccaatccctt
    42001 tccatgtacc aatgaacacc aagataaacg gccaatattt cggttcatta taccaattga
    42061 cagcttctcc tccatgtcca gtgatccact gattgaatat tcctttgtca ggacttaaaa
    42121 aagcgtaaac aaagtaactg ataatgaccc aagatagaaa ataaggtagt aaggacatcg
    42181 tttgataaac tttaacgagt cgtttatttc tcaactcgct cataatgatt gcaaatacga
    42241 ctgagatcag cagattcaaa aggataaatg ctacattata taaaattgta tttttagtga
    42301 tgagaaatgc ttgatccgaa
//
LOCUS       DAZKAO010000007        40503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000007 DAZKAO010000000
VERSION     DAZKAO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.007"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..2757
                     /gene="clpB"
                     /locus_tag="ACG8H8_000301"
     CDS             148..2757
                     /gene="clpB"
                     /locus_tag="ACG8H8_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP8985406.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2908..3594)
                     /locus_tag="ACG8H8_000302"
     CDS             complement(2908..3594)
                     /locus_tag="ACG8H8_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985407.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3572..4066)
                     /locus_tag="ACG8H8_000303"
     CDS             complement(3572..4066)
                     /locus_tag="ACG8H8_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP8985408.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4086..5306)
                     /locus_tag="ACG8H8_000304"
     CDS             complement(4086..5306)
                     /locus_tag="ACG8H8_000304"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985409.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5453..7288
                     /gene="lepA"
                     /locus_tag="ACG8H8_000305"
     CDS             5453..7288
                     /gene="lepA"
                     /locus_tag="ACG8H8_000305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP8985410.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7484..8395
                     /locus_tag="ACG8H8_000306"
     CDS             7484..8395
                     /locus_tag="ACG8H8_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985411.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9391..9642
                     /locus_tag="ACG8H8_000307"
     CDS             9391..9642
                     /locus_tag="ACG8H8_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985412.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9755..9997
                     /locus_tag="ACG8H8_000308"
                     /pseudo
     CDS             9755..9997
                     /locus_tag="ACG8H8_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10087..10326
                     /locus_tag="ACG8H8_000309"
     CDS             10087..10326
                     /locus_tag="ACG8H8_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985413.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10473..14756
                     /locus_tag="ACG8H8_000310"
     CDS             10473..14756
                     /locus_tag="ACG8H8_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFP8985414.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14855..15220
                     /locus_tag="ACG8H8_000311"
     CDS             14855..15220
                     /locus_tag="ACG8H8_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985415.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15222..15674
                     /locus_tag="ACG8H8_000312"
     CDS             15222..15674
                     /locus_tag="ACG8H8_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985416.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16123..17598
                     /locus_tag="ACG8H8_000313"
     CDS             16123..17598
                     /locus_tag="ACG8H8_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFP8985417.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17621..17932
                     /locus_tag="ACG8H8_000314"
     CDS             17621..17932
                     /locus_tag="ACG8H8_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985418.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18293..19549
                     /locus_tag="ACG8H8_000315"
     CDS             18293..19549
                     /locus_tag="ACG8H8_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8985419.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19546..20091
                     /locus_tag="ACG8H8_000316"
     CDS             19546..20091
                     /locus_tag="ACG8H8_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985420.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGAIV"
     gene            20092..20592
                     /locus_tag="ACG8H8_000317"
     CDS             20092..20592
                     /locus_tag="ACG8H8_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985421.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20951..21229
                     /locus_tag="ACG8H8_000318"
     CDS             20951..21229
                     /locus_tag="ACG8H8_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985422.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21249..21593
                     /locus_tag="ACG8H8_000319"
     CDS             21249..21593
                     /locus_tag="ACG8H8_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985423.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21612..22523
                     /locus_tag="ACG8H8_000320"
     CDS             21612..22523
                     /locus_tag="ACG8H8_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP8985424.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22602..23594
                     /locus_tag="ACG8H8_000321"
     CDS             22602..23594
                     /locus_tag="ACG8H8_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8985425.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23591..23809
                     /locus_tag="ACG8H8_000322"
     CDS             23591..23809
                     /locus_tag="ACG8H8_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFP8985426.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23822..24232
                     /locus_tag="ACG8H8_000323"
     CDS             23822..24232
                     /locus_tag="ACG8H8_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP8985427.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24284..26812
                     /locus_tag="ACG8H8_000324"
     CDS             24284..26812
                     /locus_tag="ACG8H8_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8985428.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26828..29038
                     /locus_tag="ACG8H8_000325"
     CDS             26828..29038
                     /locus_tag="ACG8H8_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP8985429.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29046..30137
                     /locus_tag="ACG8H8_000326"
     CDS             29046..30137
                     /locus_tag="ACG8H8_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8985430.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30158..30700
                     /locus_tag="ACG8H8_000327"
     CDS             30158..30700
                     /locus_tag="ACG8H8_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFP8985431.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            30895..31152
                     /locus_tag="ACG8H8_000328"
     CDS             30895..31152
                     /locus_tag="ACG8H8_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985432.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31166..31498
                     /locus_tag="ACG8H8_000329"
     CDS             31166..31498
                     /locus_tag="ACG8H8_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985433.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31543..32091
                     /locus_tag="ACG8H8_000330"
     CDS             31543..32091
                     /locus_tag="ACG8H8_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFP8985434.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32098..32550
                     /locus_tag="ACG8H8_000331"
     CDS             32098..32550
                     /locus_tag="ACG8H8_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985435.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            32647..32988
                     /locus_tag="ACG8H8_000332"
     CDS             32647..32988
                     /locus_tag="ACG8H8_000332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985436.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            33356..33547
                     /locus_tag="ACG8H8_000333"
                     /pseudo
     CDS             33356..33547
                     /locus_tag="ACG8H8_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(33707..34378)
                     /locus_tag="ACG8H8_000334"
     CDS             complement(33707..34378)
                     /locus_tag="ACG8H8_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8985437.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            34682..36313
                     /locus_tag="ACG8H8_000335"
     CDS             34682..36313
                     /locus_tag="ACG8H8_000335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP8985438.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            36316..37395
                     /gene="uxuA"
                     /locus_tag="ACG8H8_000336"
     CDS             36316..37395
                     /gene="uxuA"
                     /locus_tag="ACG8H8_000336"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8985439.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            37469..38896
                     /gene="uxaC"
                     /locus_tag="ACG8H8_000337"
     CDS             37469..38896
                     /gene="uxaC"
                     /locus_tag="ACG8H8_000337"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP8985440.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            38980..39627
                     /locus_tag="ACG8H8_000338"
     CDS             38980..39627
                     /locus_tag="ACG8H8_000338"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFP8985441.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            39747..>40503
                     /locus_tag="ACG8H8_000339"
     CDS             39747..>40503
                     /locus_tag="ACG8H8_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8985442.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLL"
BASE COUNT        13875 a         6085 c         8454 g        12089 t
ORIGIN      
        1 ttgttttttt aagatttata taagtcgaga aaaaaagtca aaaaactatg tgtatatttt
       61 gaccaaaatt gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct
      121 tacctttaat taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa
      181 gaagcgattg ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt
      241 gcccatgtat ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat
      301 gccggattgg atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca
      361 gttgtttctg gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta
      421 ttgaatgaag cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc
      481 gttattttag cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac
      541 ggattatctg aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg
      601 acctcacaaa accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta
      661 cagcaagtca aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat
      721 gtcatccgta ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt
      781 gtagggaaaa cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct
      841 gaaaatttaa aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca
      901 aaattccgtg gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt
      961 gatggacgta tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca
     1021 gaaggaagta tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat
     1081 ttgatcggtg ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg
     1141 gaacgtcgct ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt
     1201 ctgcgtggat taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca
     1261 ttagtagcag cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa
     1321 gcaatcgatt tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca
     1381 acggaattag atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg
     1441 aaaaaagaat cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct
     1501 gaacttcgtg aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc
     1561 aattctgttt cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca
     1621 gaaaataact atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta
     1681 gaaaaagaat tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa
     1741 gagtccgtta ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta
     1801 acgaaacttg tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa
     1861 cgagtgattg gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg
     1921 gctggattgc aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt
     1981 gtcggaaaaa ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat
     2041 atggtacgga tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga
     2101 gctcctccag gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga
     2161 aatccttata caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat
     2221 atcttgttac aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat
     2281 ttcaaaaata cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc
     2341 gtgactgctg atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt
     2401 ggaaacttca aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg
     2461 agcctagaca acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg
     2521 gaacatcaag aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat
     2581 gcttatgaac cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa
     2641 acaccactag ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt
     2701 actttacttg atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct
     2761 tccattgaga ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt
     2821 tcaaataaac agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc
     2881 tgcccgcttc tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact
     2941 ttcaaagtaa cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc
     3001 acctatcgta tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa
     3061 cagtgtgcca tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa
     3121 gaattcccag aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag
     3181 gaacaaccaa ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc
     3241 atagccaaga gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga
     3301 gatgatactg atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca
     3361 gtaaaacaaa acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg
     3421 catgaaaatc aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt
     3481 cgaatatttt tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc
     3541 aagtaaagct atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa
     3601 atctttttac ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga
     3661 tcaaacctat tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg
     3721 cagcgatttt tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca
     3781 ctgggatgcc tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca
     3841 gcccatctag taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag
     3901 caatcattcg actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa
     3961 gatagatcga aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata
     4021 acaaatcact tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct
     4081 ttttatcaaa aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac
     4141 ttcagtgttt cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga
     4201 taattcatca tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt
     4261 tcatttaaga cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc
     4321 caaggagcga caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga
     4381 aaccgttgaa ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc
     4441 tctttgatct gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca
     4501 tgtacccagt agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct
     4561 tcttgatcaa gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac
     4621 agaacacctc ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca
     4681 ttttcgaaac gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt
     4741 tgttgggcaa atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca
     4801 accataccac ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt
     4861 ttatcagaca tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa
     4921 taagagatcc attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc
     4981 atcaatccat ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta
     5041 taacgtgcac cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta
     5101 ttttcgtgga accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata
     5161 aaagctcgtt tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca
     5221 taggaaagca attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga
     5281 ttttctctaa aatctttttg atccattttt tcatctccat ttattagctt gataacagta
     5341 tagcaaaatt ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact
     5401 gcttttcgtt ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat
     5461 aaagaaaatg aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga
     5521 ccatgggaaa tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg
     5581 cgaaatgcaa gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat
     5641 taaattaaat gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca
     5701 cttgattgac accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc
     5761 ctgtgagggt gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc
     5821 caatgtttat ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga
     5881 cctacccgca gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga
     5941 tgctagtgaa gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga
     6001 gcagatcgtg gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt
     6061 gatctttgac tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga
     6121 cggtatggtc aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt
     6181 tacagaggtc ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga
     6241 tgtcggttat atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt
     6301 aacgttagca gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat
     6361 ggtttattgt ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt
     6421 agaaaaactg caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt
     6481 aggttttggg ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg
     6541 cttggaacgg gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt
     6601 taataaaacg gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt
     6661 aacgattcag gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt
     6721 tgtcggtgca gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta
     6781 tttagatgat taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga
     6841 ctttttcgac aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc
     6901 tggttatcaa aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga
     6961 tgcattaagc tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga
     7021 aaaactgaaa aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg
     7081 acaaaaaatc gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg
     7141 ttatggtgga gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa
     7201 gcggatgaaa caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa
     7261 aatggatgag caagatagtg tgaaataact tataatcaat cgaaaaacct attaaataaa
     7321 cctttttgag tagatgcaac actgaggatt atctgtataa aagattttaa actgtcattt
     7381 ggttatcacg gcgatactaa aattaacaaa attggaatat ttttcttgaa gatttttagc
     7441 tgtcagtata tgatgaaagc atctcatagg aggtgctttt ttcatggttg ataattcaag
     7501 tggaagggtt cttaaatctc taagaaaaga gaaaaaacta tctcaaaaaa agttagctga
     7561 tttagcaggt atttcacaaa gtactttagt taaatatgaa aaagggtcaa gaaaaatccc
     7621 taaagacgta gataatactc taagcaagat tctgaatata gaaactctaa taaaagatga
     7681 ggaagataag attgagattc tcatcggtaa actaattgca tacagagata tgaataagtt
     7741 acttaacaaa gagttagctg ataatatagg aattagtgaa gtattactca gttatgtgtt
     7801 aaatagaaag agaaatccgt caaaagaaat gcaaaaaaaa atcgatattt ttctattaag
     7861 taatgaaaaa gaaattttga aggagattaa tcgtgatagt gagatattta gtttatcaaa
     7921 agacgataaa attgttatgg gaaaaagaat acgagaagtc cgaaagaacc gggaagagac
     7981 gcttgaaaaa tttggtaaaa actttaccat ctatacaggc aaaaatgtta taagtagatg
     8041 ggaaaaaggt attaatattc cagatattga aaaactaatg aatattgcct atcttggtaa
     8101 ggtcactgtt ccttacctta tgtacggaga agattataag aatatacttc caaaagatga
     8161 aagagttagc gattttaaaa agataaattc ttttagtatg ggattaagga tgaggaaaat
     8221 taggaaagat tattatctag aaagggagga gtttggaaag cttttttctc cttcaatttc
     8281 aaaatggtcc atagataggt atgagaatgg tagagatata cccaatacta atcgaattat
     8341 ccaatatgct tatataggaa atctttcttt agaatttttg atttacggga tataaaaata
     8401 gtttacgtcg atacttatta agttatcgac gttttttatt atagaaaaaa tttataaaag
     8461 gaggtttctt gatatgataa gaaacaaaaa gggtttgttg aaagttgctt tactggcagc
     8521 ttttttctaa gtaacttttt gacaagctca atggcaacac aagcggaaga agtaatacaa
     8581 gaactaactg ttatgaataa cagctagata aaatgggcta tgtatgttat ccaacaattt
     8641 agtaatcact agaagcctag cgaaagttcg cttgataagt tatagatgag tattgatttg
     8701 tagtgtttcc aaatcaaaag ggtacagagc cacatgaaat atgttgtcct ctgcagcgtc
     8761 aggatgggaa tccataccat ggagattcgg agatctgtga gggaatactt acgtggttaa
     8821 tatgtcagtt gtgttggacg tagtaatggt catagtgggt agattgactg aattcttata
     8881 tgagaaaatg aaaccaacaa gaagataggg ttatttatac agaattgggg atcttttctg
     8941 gaaaagtaat cggataatgc gagattcgta cggtcttttt taaatttctc taaaaaaaac
     9001 ggcatcgaat gggttgggtg attgataagc aatgaatcat agactgatat agaataaaaa
     9061 atgattctat ttatcttttt acatctatcg ttttttcaag tagccgcaaa ttatatcagc
     9121 attcaacaat ttttgtatac aagttctagt aaaaggatct gaagaaaaag ttggttgttg
     9181 tgaagataaa aaaagaatca ggttttactg agtttttctt caaaatttgt gttattaggt
     9241 taggacaaag ataagaagaa gtatgaagat ttaaaaaagg aatgaggaaa agatagtaat
     9301 ttccgattat tggagttttt ttatttataa agaagaagcg aataagttgt gattcctaga
     9361 tttcgcgcgt gattaattaa tgagcaaaaa atgcggaaag ttttagtgtt taatacacga
     9421 ggacaaagtt tcttaggtat tgaaggcaca cgtgtaacgg ttgatgggtg gtgtgattgt
     9481 ttcgaggttg gaaaacatgt tgaacttatg gcatttactg agctaaaaga taagaacaac
     9541 gtggaatttt tcgagagtga tattggttgg gacgatcatg aagaagttta tggaaaattt
     9601 attttgcgga agggaaattc ttatacgaat ggggaagcat aagtagcgat ttgttagaga
     9661 tcattgatag gattgaaatt gtcgggaata tttatgagaa tctagagtta tttgaggtga
     9721 tagaatgagt gatggttttg aacaagtatc aataattaaa aatcaagttc gggagattct
     9781 tagaaaaaaa ggctatttgg tagatagtta ttttgaagga gattacgaaa catgggtagg
     9841 tgtctatgct cgtccagaga ataaacctac ctatttagat cctacgacgt cagaagatgc
     9901 ctatttacaa aataggtatc gagtagatgg ttttaagcaa gactttgcgg agtggtttga
     9961 atgggaaatt gaaaacggag aagtaaaaga agaataagag tacttgttgt atcaaaataa
    10021 gccagtagaa caaccaaacc agtagctagt aatgacaata atactgagga atcggatcat
    10081 tatggtatga ttgatatttc tcttccagaa gatcttaagc cagatcctgt aacaattggc
    10141 aatagtgtca tgatttttga ttcattagat gaagctatgt catatggttg gaaagaaatt
    10201 aaggatgaag gtagtaattg gtatggcatg agatttggtg atggtgaagt tgccaaaaaa
    10261 gctggtattc caattgggaa atggacagtt gatttccacg aagcaggttc tttttttgtt
    10321 ctgtaattat agtgggaggg taaaatcatt gatgaaatgt gttcagataa gagcgccaga
    10381 taaaaataga agagacagtt cacgtcgtta tcacttttgg taacggcgtt ttgttttatt
    10441 atataaaatt ctcaggaaaa ggaggaattc ttgtgtatgc aaaaacgaaa atgaattggt
    10501 tgaaagcact cgcattggtg gctttttctc aattctgttt cttggtggga actgctcaag
    10561 cacatgcaga agaaacgacg gctatacaac ctacgatgga agccattgcc caagtcgctt
    10621 ctgaagtgac agaagtccaa ccagcagaag ccggggtccc agctggtgaa gccactgttg
    10681 aacaggcccc tgtgacagaa gcgccaacgg tcaattccaa aacgactctc gatcagacga
    10741 ttggggaaac acaacaccca gggaatagtc ctggtacttc acaaccagtt gaagaaactg
    10801 cctcaattac aacccctgct tcagaaacgt ccactctaga aggagaaaaa gctgccgaag
    10861 caacgccttc tgtgcagcca gagtcttctg agaaacaagc ggaagaaaag aaacgaatag
    10921 aagaaacagt ggaacaacca gcggctactg ctgacaaaga aggaaaaaat gaggaaaacg
    10981 ttgaaaaaac aatctcttca accgcagcta gtcaaaatca accaacgaaa ccaaacaaag
    11041 aatctacggc tagtattgaa aatcaaaaga aaaaagagca gaacattcca aaaacaggta
    11101 cacctacact gttagtaaca gtaccaacca atcgttcggt tcgtgttcgc cgtgcaacca
    11161 atgcagctgt tttaggtggt cggaaaattg attggggcga aaatattggt gttacttcta
    11221 gtccgaacaa tctaaacttc actttaaatt atatggttcg ttttgagatt aatggacaag
    11281 tggcgttttg tattgagcct ggtgtctcag ccgcagaagg tcaagtttat acagctactg
    11341 ctttgaaaaa ctatttgaaa aattcaaatg tacggaaaaa aacaagcttg atctcttatt
    11401 taggatatgt cacaaataaa gacaaatccg atgaacaata cattgcggca caatttatga
    11461 tttgggaagc tttgggcaca aaaatcccaa tcaaaaatat ccgtgttgat tatgatcgtc
    11521 gaaaagcaga aattcaacgt ttgattaata actttggtga aaaagcaact tttaataatc
    11581 aaacacatac ggtaaaaatt ggcgaaacgt tgaaagtaaa gaataaaaat gattttttga
    11641 aatatgtgaa agacattcaa aaaccaaaag gtgtcaatgt atctattcac ggcaatgaag
    11701 tatggattac agtcacaaaa gaggcaccgg ataaagcaaa aattcaattg aataaaatta
    11761 aagttcaagg tgcgccatta gcctatcgtc gtccaggagc tcaaacaatt ggtgtgttac
    11821 atccaacaga acctaatgcg tctgttatta atttacaaat tctaaaacaa ggaaacattc
    11881 gtgtgtttaa agaagatgcg gaaactggtg tgaaagcaca aggtgcagct tctttagaaa
    11941 atgcggtcta tggtttgtat caagcagatg ggaaaaagat taaagaaatt accttgaaaa
    12001 aaattaacgg caaagtacag gcagaaatta aagatcttga tcctggcaaa tatgttttaa
    12061 aagaaatcaa agcgccaaaa ggttatgtgc tttctgatga agcaattgaa attgttgttg
    12121 aacctggaaa aactgtcaat gcgaccgcaa aagatcaagt gatcaaagga accattgatt
    12181 tagtgaaagt tgctaataaa gatttagtcg attcaacgaa tccagataac aaaccaaaat
    12241 tagctggcat tgaaatcact ttgacgtcaa aaacaactgg caaagtggtg aaaaccgttg
    12301 tgacagacaa tgatggttat gctacttttg gtaaaaatac cgttgtcttt gatacgtaca
    12361 ttctttctga aaccaaagga aaagaagggt ataaactatt tgaaccactt gaagtcacaa
    12421 tttctgaaaa tgggcaaact ttccattatg ttttagaaga taaagtaatt gaacaacgtc
    12481 ttaaagtggt gaaagtggat caagaaacag gaaaagtgat tccacttgct aatacagaat
    12541 tcaagatttt tgatacttta gccaatcgtt atgtcacaat ggagattcca aatgatacag
    12601 aaacaacgga tatctttaaa accaatgaaa aaggcttttt cctaactaac ggtacactta
    12661 cttatggaaa agatcgctat cgtttggaag aaatcaaagc ccccgtaaat tacgttttga
    12721 atcaaaaccc attagtgttc tcgattacca atgatacaga accgatcaaa gtgatccatt
    12781 ttgcgaaccg tcaagcacgg aaaagtgtaa aattattcaa gtatgaagaa tggaacaaga
    12841 agaaaacgcc gctcgcaggt gtgaacttta ccttgtataa cggtaaaaat caagcaattg
    12901 gtgaatatac cactgatcaa aacggggaaa ttttagtgaa aggtctagtt gccgatgact
    12961 attacttctt ggaaaatgcc ccattagaaa gtttccaacc aaatacagat aaacaagcat
    13021 tcacggttgc attcaaacaa aatgcgccag atgatgggga aacgttagtg gtttctgtac
    13081 gtaattactt gattccacca aaactagaaa cacaagcgtc aaataaagcc gatggtgaaa
    13141 aagtggttga tccattagaa acgatcgtca ttaaagacag tgtttcttat accaacttgt
    13201 ttgtcggaaa agaatacacc atttcaggag tattgatgaa caaggcaacg aatgcgccaa
    13261 ttttacaaga tggcaaagaa atcacaagta ccttggcctt tattgcagac aaagtcaatg
    13321 gcacaaaaga ccttgaattt gtggttaatg ctagtgttct gaaaggtcaa acgatcgttg
    13381 tttttgaaaa acttttccgt gatggcattg aagttgcagc acatgcggat atcaacgatg
    13441 aagcccaaag tgtgaaagtg aataatccaa ccgttcatac cacggctgtc tttgaagacg
    13501 gattgacagt cgcagaccca acaggattgt ttaccttaaa cgataccgtt gaaattaccg
    13561 agattcttcc aggaaaagca tacttggtca aagggatctt aatggataaa gcaaccggtg
    13621 aacccttgct tgtgcatgga aatacggtag aaacgagtgt tggctttatt gccaaagcag
    13681 aaaatgaaac ggtgatcgta ccatttacct ttgatttgac aggtataaat ggtcgtgaaa
    13741 tcgtcgtgtt tgaaagtctt tttagaaatg atgaagaact agcaaaccat aaagacatca
    13801 acgacaaagg gcaaacggta cgagtaacta acccaagtgt acgtacaacg gctacacata
    13861 aacccactgg cttgaaagag gttaatccat taacgaaagt caccattaca gacgctgtga
    13921 aataccacga tttggtcgtt ggaaaaacat acacagtcaa cggtgttcta atggacaaag
    13981 taacggaaaa accagtgcta gtagatggaa aagaagtcac aagtacctta acgtttgtgg
    14041 cagaaacaac ggatggtaca gtcaatctag actttattct agacgcacgt gcgttgcgag
    14101 gcaaagaagt cgtggtcttt gaagatctgt ttagagaagg cgtattattg gcttctcacg
    14161 cagatattac cgacaatgac caaaccatca aaatcagtaa tccaaccatt acgacgaaag
    14221 cgacgaataa aaatggcgga aaagagatct tggccttgcc aaatcaaact gtgcttgaat
    14281 gggtcaaagt agacggtctt gcgattggtc aaatctatcg cttaattgtt caaggctact
    14341 taacaccaga cggcacacca tttgaaggca ctcatgcgga aaaagtcttt gcggccgata
    14401 aagaaatgat ggaattctta tttgaattct tagtggacgg tcgcaatctt gcaggaaaag
    14461 gcttgagttt tgcggaatgg ctccaagcga aaacggaaga tgagaaagaa acgtttgaag
    14521 aagtggcgaa acacaatgta gatctaacaa acaaagatca aacggttcaa attgtggaag
    14581 cgccaaaacc accgcaacca gtgaaaccag aacaacgggt taaaccagct actgttgtac
    14641 caatgttgcc aaaaacaggt gaacaaacaa gtagtatact tatggttctt ggattagtag
    14701 tattagtcat tgctttaggt ataacacttt ataaagtacg taaagccaaa gattagtcat
    14761 gaggaacaag cggttaggat ttttaaccgc ttgtttgatt gtaatgtaga aatatttaga
    14821 atttattttg gtttttaaga ggaggaaaaa ctttatggca gaaaaagcat tgcgtatgaa
    14881 gaaagagttt ttaggtcaag tatttttatt gggttcacgg gaacctaatc caccaaagga
    14941 aattaatggt gtgtctgggc gatatgttca attacaagca gagtgttcgc gagaagaaca
    15001 gcctgctttt tttgtttatc tcacagctga agaggataaa agattacgat taccagaaaa
    15061 agaggtgcgt atttcatttg aagaagctta ttttgttttt cgtaaagaac gagtgaatag
    15121 tcgtgaaaca aatgatatgg gacaaacaga gcgtcgaaca actggctatc aagatgttgg
    15181 tgtcttttta actggtgtaa aaattgggga ggttttgtaa gatgggatta aaatttccaa
    15241 ataattatga cattacagaa gcattagatg aaacttgttt aggagccgta cgctttggcg
    15301 cattagaaga gtctgatcgt gtatttgata atgaaacagg cgattatacg ggagaaattc
    15361 gatcacagcg agcacaatta gtttttggaa atccagatga accaaagatt gcacgacgag
    15421 ttaaatttcc agcagatgta gatttgactg gttttaaacg acgtgatcct gtgaaattaa
    15481 ttgatccaga atatcatgtg gtatatattc cagcacaaga tggcaatcgt gaatattcag
    15541 atttagtgat ttatgcgaaa ggattagaga aagtagaagc tccaacaaat aatcctaata
    15601 tgaaacaatc acaagaaaaa ggtttagatc aaaaacaacc aagtgttgat tctaaggaaa
    15661 aagagaaaaa ataaggtttt tttgaaagaa taggaaggag tcttcctatt ctttttatct
    15721 aagatagaag gaaggggaga ttttgaattc agataaaacc aatcaaatac tttgagagat
    15781 tgtgcttgtt atcaaaatat ccaacaattg attgagtatt ttggagacaa tccaacgatt
    15841 tttgtacaaa caaatgcata tatttgtgcg gttattgggc agtgtatgtt tttacgtgat
    15901 cttgttcgtt tttcattacc gtaccagtgg aaacaagatc atcaggaagt gatttggatt
    15961 cgtatagtac gaatatgtaa tatgtgtgca cacccttata aaaatcgatt aaatcttttt
    16021 cgattatgtt tttttactaa atgtgatatt cctgtattag ttgctttttg tcaaaagttt
    16081 ttagcacagg aaaagtaaaa acagaaagga ggaatgtgac aattgaaagc tcattttttc
    16141 aagtatcgtg gtcggcgaat tcgctatttc aatcgttatt tatggttttg ctattatggt
    16201 ttctttttgt ttccattttt gattctgtta ggttggttta gttggaaagt attttatcca
    16261 cgtttaagta cgttttcgaa ttggcaaatg tatcttattc ctggaataag tattgtgttg
    16321 tatttgttgc tggttagtgg gttaatttta gggttgatgc attggtcgcg actaaaagaa
    16381 ggatattttg cctctgttta ttggcgtcag ttacttgttc gaatgttaat tgacaatcgg
    16441 tttgtttata caaagaaaca agttagtgag aaacaaacaa gggaaaaaat gagattacca
    16501 aaagtttatt tttatcaaaa gaaacaagaa ttagtggttt cttttccttt agatggtggg
    16561 cgttttcacg aacgattttt aaaaatgggt catttgttat ctgaagtatt tttgcgtgat
    16621 ttaattcgtg aagaacgaga aaaagcccga atcaattatg tattcctttc agattctggt
    16681 cggattccta ttgatcaatg tatagcagaa aatggacgta ttcatttaat gaaaactttg
    16741 gattgggtat atgatcaatc accaaatatg ttgattgctg gtgcaatcgg tggtgggaaa
    16801 acctatttat tgtattcttt aattcaagct tgtttaagtg ttgggacagt agatatttgc
    16861 gatggaaaag cggcagattt agcagcatta ggtgacgtcg atgtattcaa aggacatgtg
    16921 ttttacaaaa taaatgaaga aatgattatt tgtttacgaa atgcattaaa agaaatgaat
    16981 caacggtatg cctatatgaa aaccatgaaa gatcctaaat atgaaccagg gaaaaattat
    17041 gcttattatg ggttaccacc gcattttatc ttttttgatg aatgggcagc tttttatggt
    17101 tcgttggata tggaagcgag aaatagagtc gataaattga ttcagccatt agtattaaag
    17161 ggtcgtcaag cagggatgta cattatttta gcaatgcaac gaccagatgc agaatatttt
    17221 ccaagtggtg ttcgggataa tttaacattc cgtatttctg ttggtcgatt gtctcccatt
    17281 ggttatttaa tgacttttgg agaagatttt aaaaataagc cattttacaa caaagatcaa
    17341 cctggtcgtg gatatgcttt tccaggattt gatgtacctg tagagttttt tgcgccattt
    17401 gtttacaaag attatcactt tatcaaagaa tttgagaaat ttactgaaat gattgtctat
    17461 gattttacag taaaagagga tagtcaagat caaacgatgg gagatgataa tcagttgtta
    17521 caagaacaac tagagcgcca aatgtattta gcaaaagatc caaaaggaac aagtttttct
    17581 gatgcgcata ttttgtaatc aactaaggag gtgaagttaa atggcagaga gttgttatta
    17641 tagagtagct acagcaatac gaatgattaa tccttctctt tcttccagaa cgttttatga
    17701 ttggttgaat cgtattgaac aagtaactga ttaccgcttt ttgcgcaaag aacgtgtttt
    17761 tacaggaaaa gttattaatc aagtattgct aacgaaaaaa gatatagaac gattgactcg
    17821 attgtatcat tatcgagtag atttagaaga ggatttaact ttgtcaattt atcgcgtttt
    17881 ttctcctgaa aaatatagtg aaataacaaa attagatcat ttaattttgt agttattatc
    17941 tatcgggagg gctctagacg tagtctggta gccgcactct gcctggcggt gcgcattaag
    18001 gctgcgaagc atgggcctat aatgcgcact cgctagcagc gtgtgcagct cacaaagagg
    18061 tcgtgcgctt gcgcacggcc tctttgtgta aagccccccg atatataata tggggggcag
    18121 aaataacata acacatagaa aaaagtttta taaacccttt tgtatcaagt gattatctaa
    18181 ggtcagcagt tgtactactt ttcccaaaaa acgaggggga ctagttgata tatcggtgtt
    18241 tctaaagggt taaaaaaaat cgattttgtt attttataaa aggagcgaga aaatgacacc
    18301 gattgagcta aaaagctaca gaaagaagtt aagtgcaatt cataatataa gtggagataa
    18361 aaagccgcta tcacaagcaa gatttgcgca attgatgggc tgctcaaaag attacattag
    18421 aaagatggaa caagggaaaa gaaaaatccc agaggatttt gaacaacgaa tcaaggaacg
    18481 ctttcgattg tatatgaaaa gcggaataga tcttcgagca atgattgatt atttgcgtct
    18541 ttcctttttt gggacagact acaaaacagt gattcaacgt gtattacgaa tggatctgac
    18601 agaatttgaa acacgtccaa cgggcttgta tctttttgac tgtctttctg ttcgtgggaa
    18661 tatctgggtg ttctggcatt caaaacgaac tgtaacagaa aatgtacttg tgcaattttc
    18721 aggacaaggt tgccgggaat atgaggaagt catgcgtgaa caacaaacag attggcgaac
    18781 atggctatat tccatttgga aaaatcaagg aaagttggaa gctggttata cacgtgtgca
    18841 atgtagccga ttcgatattg cgttagatga actttgggga ttggaaacgg aagaacattt
    18901 cgatctcttt gaattactag aaaaacaaaa agcaggttta ttagaaatgg actttaagag
    18961 attccagaat attggcggtt gttttttaga tgatggctat tttcgaagtg aaggattatc
    19021 tttgtatttt ggatcgcgaa agagtcctct cttttttaat ttttatgaga aacgttttga
    19081 aattgcgaac cgagaaaaaa tttcagaatt agaagcatta acgaaatatg gtatttataa
    19141 tcgatatgag ttacggttag ccaatgagaa agcaacacaa gcagtagaag catttatttt
    19201 aggtgattca ccgaaacgtt taggagagat tggcgttggt ttaattaatg caaaacttat
    19261 tgtttgggat agactggaaa gtgatgcacg gattgttgat aaacgatggg caaaattatt
    19321 tcgttcgatg gaagaattgg attttacagt aaagccagaa aaattctcat tagatcgttc
    19381 agaacgctgg tttttgaaac aagtagctcc gacattaaag ttgttacaag aagcagatcg
    19441 tattttagga agagactttg tacaagaagg gattgataat gccgaactaa atgacgagca
    19501 acaaaactat ctagaatgga tgggaacgtc aggagggaaa attgcatgaa tgcatatacg
    19561 acaacagaat ggctgcgcct gttggattta aaggctgcaa tcgaagcatt aaatgaaaag
    19621 atggttgatt tgtcttattt tcgttttcga gttccttata ttgaacaagc ggtaaaagct
    19681 ggacgttatc aggaaaaaga gaactggcaa gaaattgcac gcttattaga agttcgtaag
    19741 ggatacgaac aagaactcga agagttggaa tttagccgaa ggaaaggaag gctagaattc
    19801 attcgctttt atcgattttc attgccaata cctgcgattt tagcggttaa aaaaggctgc
    19861 gataaaatga agatatatga aaactgtgtg gcagcactta gcagcgaaaa acctcttatg
    19921 gaagaaattt cattggtgac tgtcacttgg gaaatgagtc gccagccaac agaagaacag
    19981 acatatcttt ctttagaaga gattgagata gaattagagg aaattggtcg ctatgccact
    20041 tgttctacct attgtggatc ggtcattagt attgctggtg cgattgtcta aatgaaaaaa
    20101 attatgattt atgttggtgc gtattggtca cgtgatccaa cagtattaga aaatccagag
    20161 gcggtacact attgcttgcg ccaattattc tatctgtata aagaacgatt agaatcgttg
    20221 attcgtcagt taccatatac tgacagacgt ttagatgaac ttcttctaag gtatccagca
    20281 atgtataaac gacgaaaaaa tagattgtta ccagaagagt atcccattga aaaacatgaa
    20341 cttgaagggc gatttgtggc ttatttctat gatgatgttc gtatgcgctt agtagaacaa
    20401 cgaatggaaa tagaaaatga tcgctactat tttatcaatt attgtaagaa gcgtaagtat
    20461 caagtaactg atgattttta tgattgtatt ttacaagatg gggaagtgat tctatccaaa
    20521 attgctccaa gttttcgtga acttgtgccg attgattttt tgaaaaaatg tcatttgcgg
    20581 attcttccgt aaaaaagagc tataaaagga aagaaaagta gaagccaaat cggtttctgc
    20641 ttttttattt tttttaggaa ggagaagatg gtatgtcact ggaattttta ggagagccaa
    20701 ttgaaaagga agagttcgtt tcgcattata tggatttgtt tattcaggtg ataaaaaact
    20761 ttgtgcgatt gatgaatttt atccacgcga acaagactat ctagccgctg aacgaataaa
    20821 aatcagttta ttatgggaaa aattcacaga tgaaaataac cgaccgccag attatcgcta
    20881 tttatcagat tgtttaacga ataatgtgct ttctaaagaa tacctatttc acaaacgtga
    20941 gaaaaatcaa atgtcggttg aacatttttg tgattgttat attgcattat tgcgtagtca
    21001 acagttatta acagtagaac gatatggtgc agagtttggt tatgtcatgc aagctgagcg
    21061 tcatagagct acaagatgct atgaaaaaga agatcaagtg attgatggct atgaagaatt
    21121 atgtattcga aacaatcgct atttgcaaca acgtttatat gaacaattga tgatggattt
    21181 ttcaaatctc tatagagggt atgaatgttc gatgattcaa gaaaggtaga agaaaagggg
    21241 gaatttttat gtggcgatta ttatttttgg tggtatgtgg tatggcattg ttctatgtgt
    21301 atcgtgtcta tctctatcca cgaatggaac gatggacaag ttggtataga gatcctttaa
    21361 aacaacgaaa aggattgaag cggtatcgtt atgaattact tgttgaattt tcggatgggc
    21421 gacaagcaca gtattatttt tttgcaaact atccaaaata caacaccagt gaactattga
    21481 ttcaattatt caaaaataat ccatatgttg ggttagaaga aaacggacgg gagttctttt
    21541 atcgttcaga taatattagt gcggcgtcta tgaacaaaac acgaattgca taaaaaattg
    21601 gaggaataat catgaaaaaa acaacattag cagtcgtaac aacactagga atttcattat
    21661 tttcgtttca attagggagt tcattggttt atgcagatga aactgtagaa acacctaagt
    21721 cagaaattgt agaaacattg ccaggggaag gattaccaga tgttgagaca gagctaaatg
    21781 gaggaacaac ggaaaaacca gcagaacctg aacaattgca gtcagagaaa cctgaaaaag
    21841 agccagtgac accagaacca gagtttcctg aatcaacttt accgactgat tcgatcgaag
    21901 aagaaggtgt gcatagttca acaacaacgg attcaagtat agtcacaact ccggaacaat
    21961 caggaacaac cagttcttct gaaaagaata ctgattcgtc aagtacggaa cagccaacga
    22021 tgtcgtctac cagtgaagaa caaaaagaag aaccgaaaca acctgttgaa aaatcagaag
    22081 caattccaaa agaaaataaa cctattgttc cagcaaaaga agtcacagta tcggtaaccc
    22141 caagcggtga aattacgacg aatacttctc aaggaatgag tgtaccgatt gtcactagta
    22201 atgtagagga attgacccat attccaacac caaccacccc attaaaagta gcaggtgatc
    22261 aaacaattgt tggtgtaaaa gatggtattc cattaatcca agatagtgaa gggaatttgg
    22321 taaaggattt gtcgattcct gtaaagaaac ttccaagtgg caatattgaa atcaaaacag
    22381 cagatggaaa aacaaaagtc ttaccaaaga ctggtgaaga aatacatgtt gcactttctg
    22441 ttctaggagg agtgttgaca agtattgctg gatttgttgg atataaaaga aagagaaaag
    22501 gaatagaaaa tgagggtaat tgaatggact atttttcaga aaaacaagct tatcgttgtc
    22561 aatcgtacgt actagtaaaa acatactaga aggagtagct tatgaaaaaa cgacaactac
    22621 ggcggaaaga aaaagtttat acagagaaag taccgaagca aaagaaaatc cattcaaaaa
    22681 aagcagtgaa atggatttgg tgtttggttt tatttctttg tttttcaggg ggcttggcat
    22741 tttttcaatc catgaaaacg aaggttcaag tgtcgttttt gaaagatgaa atccaacaat
    22801 tacaaaagaa agaagtacaa agtcggtcag aattaacgta cacacctgaa attgaatcct
    22861 ttatgaatcg ttttgttgct ttgtatatga atacattcga taatcatgaa caagaacaac
    22921 aacgtcaaga aacattaaaa aaagagtttt atgcacaaaa ctttattcaa gaaccaggaa
    22981 gtgtttcagg tgttcgaaaa ttaactgctg cttcgtttat tggattaaaa gaaattgaaa
    23041 ccattccaac tgctagttat aaagtaactt atgatattcg ctcgatgaat ccagagagta
    23101 agaaagaaac aagtgtgaca atcacacaag tactcaatct tcccttttct atttcaaaca
    23161 agggctgtcg tattattgct tatccttatt ttacggcact accaaagaat acagaaaaac
    23221 aacgaatgat tacttatgat gacacattat acgaaacggt tgatgcgtcg gaagcgaaag
    23281 atgttgaagg atttgtggaa gatttcttag ataagtatgc gaaaaacaat gcaaaagata
    23341 tgtcatacat gatgaaagaa cctgaaggac tcaatcaaac agctaaagtt gatcaagtag
    23401 agatgaaagt attgcgttat aaagaaacga aaaaaccttt tattgtaaaa gcaacggtcg
    23461 tctttcgaga tggtgatacg tcacttttac aaaaagaaca aatgacgatt gtcattacga
    23521 aaaaaacaaa tcagtattat gttgaaaaat taacacataa ttggttggat aggaaaggag
    23581 aagaacggaa atgacactga agggaattgt agattttata ttaacgcaag gtggctattt
    23641 tttaataatg gtggttgttt atctgggatt gcgaaatttt atgaaatcaa aagtaggaaa
    23701 tgcattaata tctatcgcag caggtgccgc agtttatttc ttcttaaatg atccaacacg
    23761 tgtatttgat gcgtttggta aaatcattga aaaattattt atggattagg aggatagtga
    23821 agtggattgg gaaccttatg attattcgat tgtttatgcg gaaccttatc gcgtacaaca
    23881 gattacaagc aattggcgtc ttccatttcc agttgcatta gtcaatgcag caactttttt
    23941 gttggtcgta atctttgaat atttattttt acgttcattt attggttggg tcatttcttt
    24001 ttttccacca gcaggagttg ttttttatat aggactcccc tatttatgtg tgttagcact
    24061 ggcaaaacta aaacctgatg gaaaaaaagt gcatttttat ttatgggatt ggttttgtta
    24121 tatgattcgt atgatgaatg gtgattgtgt gtattataac tgggacaagg tatcaaacaa
    24181 tgcattggaa gaattacttt ttgtgaataa gtatgtttat ttagaggatt agaaaaaggg
    24241 acaatcaact ggttgttcct tttttattgg gaggtgaaat tcattggtgc ttgtaaaaga
    24301 accagtaaaa gcattcaaga aaaattgttt gttaaatgga gaggattgta tctgggccta
    24361 ctatcatgtt tttccacgaa caatttcaaa aaacacacca aaaaagacac aggaaataaa
    24421 agaaatgatg gctcgtttac tgttgctttt agcagattat gaagatgtgg agttggtgat
    24481 gtatccacga acaatgaatt tacggacgaa gtttgacaca ttaggaaagg gattttctcc
    24541 cgatagtcaa gcaattggtg cgtattatgc aaaagaaaca gtgacattgt tggaacgaga
    24601 aggtcaagtc tatcgtcctt catttatttt aggaatcaaa gtcaaagaaa aagcaacggt
    24661 ggagagtttt catgatgtaa taaatctaat ctggttagat ttagcaggaa gtttagcacg
    24721 cttatttgga ttagaagtag atgatttgga aactttgttt tctgaaagtg atccttatgt
    24781 aaaagaagtg acacaattac tttatagctt ttctattcgt cccttagtaa aaaaagagct
    24841 gttgtatctc aatcgtctgc aatatattcg aggaatggct cacaaagtag aagaacaaaa
    24901 taattgggaa gtactgaaag acaatcaagg aacgtatgta cgtataccga aggaacataa
    24961 aggccttctt caattagaag atgagttagg gagtagttat ttagcattgt tgcctattgg
    25021 ggaaacacct gaaaatatga gaaattgtga tctatttcaa tttgctcagg atctttcttt
    25081 cccagcagaa ttgcgaatga aaattcacta tccacgcttg aaaggacagg gaggtattgg
    25141 tttaaaactg aattggctat cggaacggtt caaaacagag cataaggaat tggttcaaaa
    25201 tgatgaagaa cctagtgaac gcttattaac tgttcgagga ttagtaaaac atttaaagca
    25261 acgagtagat aatgggcaac ctgtcgtcga ttggatcgca tgttttattg tatacggtca
    25321 aacggttgaa gaagttcgtc aaaatagtgc aacggttatt caacaacttc atacgattat
    25381 ccatatccac cgtgcaaaaa atgatcaacg agaattgttc tataaatgtt tattagggca
    25441 accattggga cggcagttac attggaagca ccgaacaact gtagaagcag ttggcgaatg
    25501 gttgttttca gcagtatcag aattaggtat ggaatccggc tggtatattg gtcggcaaga
    25561 tacattaggg gatggtggtt ctcgaggaga aaaaacacca ttagaagaat taatttatgc
    25621 gtcaaatcgt tttgtctttt tgaatcttct agccattgcg gaaggaatca aaggtgcgtt
    25681 gtttgatgcg cctcatgtag cagtaactgg aaaaaccggt aaagggaaga cctttttagt
    25741 tggcttgctc tttttgtata gttcgtttat gaatgtacaa agtttatttg tggatcccaa
    25801 aggagaaaag cgcaaatggt ttacgaaact cattcaagac ccgtattatc agaaacatta
    25861 ccctttgttt gtagaacatt tgcaaaaaat gagttatgtc acgttagatg ctacaaatcc
    25921 ccgtaattat ggtgtattag atccaattgt ttgtttatcc cgagtagatg cgaaacaagt
    25981 agcccaggat atgattgatg agttaagtcc gttaggtacc aatcaccttt taaaaggggc
    26041 ggtattaaag ggaatccaag aagttttagt tcaaaaagaa gccggacaac aagttggctt
    26101 gatgcatgtg gtcgactatt tagaacaaat ggaacaaaaa gaggttcgag aatatggtga
    26161 gtttttacgc ttgacggtag aagattcgat tttacgttta ggattttctt atggagaaaa
    26221 cccgggatta gactttaatg ccaaaacaac gattttagag atccaagatt tgaaattacc
    26281 aaatgacaat gtccgaccag aactatatac agatgcggat cgaaagtctt tgtgtttgat
    26341 gattagttta ggtcgttttt gtgaaatgtt tggaaaacga gatagtagta agaaaacagc
    26401 gatttatttt acagaagcat gggtatttaa taattcgaac gcaggacgta gtattattgc
    26461 agcaatggca cgtgtggggc gttcccagat gaatcaatta gtgttagata cacaatttat
    26521 tggtgactta ggttcagata aagaaaaagg gaattttggt gtcgtctttg cttttgatga
    26581 agacagtgag cgagaaaaga ttttgaatca tttacgtttg gaagtaaatg aagcgaactt
    26641 agctgaaatg agccacatga ttaaaggaca gtgttggatg ttagatccct atggacgtgt
    26701 aggaaaactc aatgtacaca gtttgtttga agaaatgacc gctgcattac aaacaacgga
    26761 aaaaacaagt cactctcaag tagaagaaca ggatacgtta atcatgcaat agaaatgagg
    26821 tgaaacaatg gcttatataa agcataagga aaagaaatgg tgcgttttgt tttttttaac
    26881 gattggattc ggactgcttt ttgttggtgg attggtgtat gcggcagaaa agcaggtgga
    26941 tcaatcgatc tatgagattg ggaaatttca tattgatacc ttttcttctt atttatctga
    27001 tagtgaattt tcattaggca aaatgttatt tggggcaaat ggcacagatt ttctaaaaat
    27061 gatgacgaat atgttcttta gtttaagtaa attgatttgg caaggccttg attttgtcat
    27121 tgagaagctc tatgaaggag ctgcaatgaa cgagttaatt caccagtttt tcactttttc
    27181 ggatactatt tattcaaaaa tatacgatcc tattggtgcg gggattgtcg tcttgtatgt
    27241 gttatatcta tttactcttc gtatatttag agggaaacat tacgcaaaaa tggtgtttat
    27301 tcgatttctc ttcgttgtca ttttttcaat ggtttggttt ggctatggta gtaacgtacc
    27361 tagtaaagga gaagctttta ccaaagggtt aaatgatttg tccgtggagg tagaaggctt
    27421 tattttttct gctacaaata gtctagatga attggcgaca aaagatgtca atggtgtttt
    27481 agtgccaacg acacaaaagg aagccattca aaaagtgcgc caactttatt ataaagcggc
    27541 tgttgttgat ccgtatatat tgttaaatta tgggacaaca aatttagaag aattgtatca
    27601 agcagagatt gaaccacaag aatttctagc caaggaagcg tcaagtgaag aattatctga
    27661 tattgagaca aaagtgacca ctgctgcagg agaagataga gggaaaaaga aggcttatcg
    27721 tgtctatatt caaccaacta aagctgttta taaagcagtc gttggttcta tggtgccatt
    27781 gcttaatcta agtattggat taccacttgt agtgattggc atgattcgtt ttatctttca
    27841 aatgttggtg ttgtgtttat tacttgtgat tcctttttca ttactcgctt cttttgttcc
    27901 agtatttgat tatatgctca tgaatacctt aaagtccttt ttgggcttga tgtttcaaaa
    27961 atctttttac agtgtgatta ttttggtggc atttttgatc tataacatag tagacgattt
    28021 aattccaatg aatactgttt ttggtttttt aggaaatatg tttgtcaaag cactcctatc
    28081 aattttactc attgtaaaac ggaaagattt attaaagaaa ctagggttag gagctgcaga
    28141 tcaaactata aacaacacga aaaacatagc aagtcattct ttgtctcaaa tgaaacaact
    28201 gcctggtcaa ctatcaactg ctggacaaaa tttggcaatc aaaggtgcag gattagcagg
    28261 gaatgtgtat cgagcaacga atccactcgc ttctgcagcg atacatggat ttcaaaagtt
    28321 tcaacaaaaa cgtacaccac aagaatcggt aaaagggagg caagtggtta atccctttga
    28381 actaggtggg cgttcaaatc aagccttgcc ttatagtggt cgtcatgttc gttatcatgg
    28441 ggtagagcct gaaatgtctt ctaccaaatc gaaaacagag tggaattctg gtctttcaaa
    28501 tggtttgatt ccaaattcgt ccatgaattc agtaagacca aaacaacagg tacaatcatt
    28561 ggcacaattg agtaagcaac aagggaataa tcaaagaaaa ccattgttaa ataaaggtat
    28621 cgcttttgtt ggtcaatcga ttccgttgcc ttctggaaaa gtacccgtgc cacaattaac
    28681 agctgctggt tctctttcac aacagattcg ccaagtgaaa aatcaggcga aaaatgggag
    28741 ttatccagca atgcagcagt tattgaacca atactctgtt cctaattcgt cggtagccac
    28801 tctatcagga aatcgttctg tgcaacaacc ggggcaacgt tcgtctgaaa gtaaaccgtc
    28861 gatttccgca ccaaaatcgt tacaacctgc attttcttct ttagataagc caacagagca
    28921 atcggaaaaa atagaaaagg atcctaaaac actgccgatt ttggacgcaa aaccaggaac
    28981 tgattttact ttaccaaaag gacgaacagt ccaaaaacca ttatcgaaga aaggatgagg
    29041 aagaaatgaa gaaagggtgt ctaggttgtc ttcttttctt tattcttccc ttttttctat
    29101 tgccattctt tttttcagga gaactttctg agcatggagg agaagctaca ggtctagctg
    29161 tcgcttttaa tggggaatat accgaaggat taccgatttt ttcagagatt aaaggacgtg
    29221 gtccaatcac agatgaacat gcgcaagcag cagttggtgc agctgtaaag tatcagttat
    29281 taccgagtgt gattctttct caattaggct atgaatcagc ttatggacag tcacaatctg
    29341 gaagaaacga tacgaacttt tttgggatta cctggtttcc tggttgtccc tacccacaag
    29401 gttctgcacg tggaattggt ggttctgaag gtggtttcta tatgaagttt ccgaacttaa
    29461 aagcttgtta tgactattat ggctttatgg tggcgagcca aagcaacttt aatcaatgtg
    29521 tgggcaataa agatcctgga agttgtttat tgattctagg tcgtggaggc tatgcggcag
    29581 caggtattac cgaagggagt ccgtattatc gaggagcaat gagtatcata acgtcctata
    29641 acttaacgga atatgatact tttgcgatca agcattggaa tagcctacca acaaaaaaaa
    29701 cttcgcttat tgcaggaaaa agtaaaggca atgcgaaggt tttggatcgg atcattggac
    29761 gatccgtaaa taatggggaa tgttatggtg gtacggcatg gtatgtatct caattaggtg
    29821 gcccacagtt aatgggttct ggacacatgt ttgcgatgtt aataggggaa gattataact
    29881 ggacaaccta tggttggcaa gtaatcatga acccaaaacc aagtgattta caagcaggag
    29941 atgtggtgaa ttggcaagca ggtggtgcat tgtctcctgg aatctatggt cacaccggaa
    30001 ttataacaga tgtttcatat ggcggacaac atttctctag tgtggaacag aatgccgagc
    30061 aaggacggat tatggctcgg tatcaacgca cgtacaatca aacaaaaatt cgtagtctgg
    30121 ttcgtaaggt gaaataagaa aggcagtgac tagaaacgtg aaaaaaggga taattttagt
    30181 atttagttgg ttatgtttga taggtcttac tggttgtcat tggttttctt ctgaaaaaca
    30241 aaaaacacaa ttgagaacga cagaagaaac aatcgaagca gtaaaagaaa caagtggaac
    30301 tcttcaaagt actgaaagta acagtcaaat tggttcatta gaagacgaga cagcgcaaca
    30361 aatgcaaata gatgaaatga caaaaaattt cttaaaagat tttgctagaa aatggagtaa
    30421 tttcacagat gtttataaac gcaatcaagc tgtgagggac tatatgacag atcgttgtat
    30481 taaagacaat agtattgatg tagacccgca tgtacaaatg aaaacaactg ggaaacttta
    30541 ccaggtgaca caagatatag agaatccgga tcaatatttg ttatttggag aagaaacaat
    30601 gaataaggtt actcaatttg tattacttca attgaaagta gatcaagaag ctcaaaagat
    30661 tgatgcgatc aagatttatt atgtacggag tgcgtattaa aataaaaaat gaacactcat
    30721 atttattata caccgaagaa atagaaaaaa acagactgtt tttttaagaa aaactatgtc
    30781 attctttact tgaaaaaagt tgttatagga cagaaaggat aaaacaaaaa cggaaaaaat
    30841 atgtcttatg aataactata agaactattc tattttttag gagatggata cgagatgaaa
    30901 aaactaattg gctatagtgt gatgtgtatt ggatttgttg gtgtgggatt gtttgtatcg
    30961 actgaatctg cttctgcggc tgaatggtat aatcctatct ggtataatcc gaacattgaa
    31021 tgtcaaaaag gctatccgta tggaccgaca aatctgaaaa atggaaaatg tagagtaaat
    31081 tggaatggcg ttgtagatag tgtaattact ggttcaatgg actctgctct acatggcttt
    31141 acgggtcact agaaggagga agaaaatgtc tgaagagaaa aaagaaatcg ttgaatcatt
    31201 agtagcttta aaagattctc tttctaagat tgaatatgtt gataggcaag aaattcaaaa
    31261 actaattgat gatactatta ttgaaatcca agatgcgcgt tgtgaaggta taaaaatttc
    31321 tgtagcttta tctaaagtaa tagaaaaaat gaaccgttca ttagcgttta atggattgaa
    31381 gctagataga caaactagtc tgatatggga tcatttaaaa gacttgtatg acaagtctaa
    31441 aagaagtgaa cggactgctg tatcaatctt aaaaggcctt tggggaatga attcataaac
    31501 aagatattta aaaagtaact tattttttat ggaggagaaa ttatgaaaag aataattaaa
    31561 atatttggtg ttttaggtgt tttcttatta ggtttaagta cttttgttag tactggaaca
    31621 agtgtgtatg ccacagaaaa agtgaataca gttgatttag aaataaagaa taaattagta
    31681 gatgaattaa ctttttattt tgatacagtt ggttttttag acgaacatgg tgtataccat
    31741 ataaaggatt ttggtgctat tcgtgaacga atcagtcaag ataacgaaca aggaagagca
    31801 gcaagagtct tttatgaaca tattattgtt cctagaatgg agaaagttga ttggaaacga
    31861 tatgctggat gtgtagccat taattctctt cctggaggag gagtggcttg ggctataggt
    31921 acgcagggag attttattga agcattaaaa gcttttaatt ttgaaaaagc tgcggattta
    31981 ttattaaaaa ttgcagaaaa acaattagga aaagctgcag ctaaagagtt agctaaggta
    32041 aatattgtag caggaattgc agcagctgct gctagctgtg cattagagta aaagtttatg
    32101 aaaagtttag aacaagagca tatggctttt aaacaagcaa tgtttaaaga aaatatctat
    32161 ttaaaccata actatattcg tgtttctaag gcatgttcac cagtattgaa tatgcttgga
    32221 ggtggaaatg gattatatca tttactgttt gtcgatgttt gttggttaat ttttttacca
    32281 gatgaactag ttatagtcaa tgaaaaaata acaagtaaaa atgaagtgtt taattattct
    32341 ttaacaagaa ttaattacaa agagattacc aagttttcac tagaaaaagt tcctttttgg
    32401 ggagaatttt gtttaaaaat taaatgtaac tggaaaagaa tgtatttcta tattgatggt
    32461 gatgatgctc ttacattcgg taaaactact ttttctagtt ttaattttca gtttctatta
    32521 aaaaataatt tttatggttt attgaaataa ggttgcgaag gaagatgtag agttatttgc
    32581 actgttgtgt tggtatatct cttaatgctg tgtctttttg gggagattaa ttagtggagt
    32641 gattatatga gtatagatga ttgtttagaa gaacaaataa gattattttt aaagttttta
    32701 aaagttaaag ttcaaaataa ctacttcaca tgttatagtt atgcacaccc tgttttaaat
    32761 agtcttcctt ttaattcttt agtgcaagga ttgattacaa atattgtttt attagtctgt
    32821 acaccaaatg aaatatttgt aaaaaagata ggcagtggta tttcttcagt aagattaaaa
    32881 gagaaagaat atactcaaaa gattattcat attcctaagc ctgaaattaa gcgttttgag
    32941 ataaaagact attctgcttt agggctgttc tcaattggtt actggtgata aaaaataaga
    33001 atactaaaac tatacgtaga atataagtaa tcggcatcaa tcctaattat gtgattgaat
    33061 gccgattttt gttacgcgct atttttaatt atctgtaaga agaataattt tattttactc
    33121 atattcgttt tcactttgaa gaagtaaatt gcaatttctt ataagttaag cagctatgca
    33181 aaaggctatt tattctattg tgaaatataa gatgtagttg aaacaggata tatagaatca
    33241 aaatacgaat cttattttga tttttctagt tttttgaaaa aagcaccctt tgatcgttgt
    33301 attagtggaa aagagaaaaa ggagcatact tatcatgtca caaaactaca actaaatatc
    33361 taaacaacac caatttgatg cctattgaaa aaaagtattg cgaaatgaag ccaagtctat
    33421 tagaaaatga aataatagaa taaaagaaaa tgggaaaccg ctgaatgatg taaatgaagg
    33481 taagtatcca cgaactaatt ttgatgaaca agatatctat tttttatttg tattggagat
    33541 actagtttaa tatcaaaagt tatcagcagt cataattagt tatccaatgt aaaaagtaca
    33601 aacaatagtt gttatgcaga atagtttatg aaatatatta cataataaga aaacaagcga
    33661 tatttttctg atcagaacaa ccatttaatt taaaaagagt taaaagttat atatttttga
    33721 aatattttgg atgagtagct aaaacaatat tttgctcatt aagcattaaa tgaagatgca
    33781 acgatgttag aaagttagct tcttctggat cttgttttgt taatgcctca aatagttgat
    33841 aatgttgttc cataattttt tcccacttta atccttccgt ttttactcgt agccatcgaa
    33901 atcgttctaa gtgtgtattg ttattttcta accattgcca tacttcttct cgctctgcaa
    33961 tttcaaaaca agctttatgg aataaattat cattatgaaa aaaactacga gcattttttt
    34021 gactaacagc tttttcttgt tctgaaatta tagtttctaa aatccgtttt ttttggttat
    34081 tcattttggc acaacactct aacataattt gtcgttccaa atgttctctg acaaatcgag
    34141 catttttggc cgaatctaaa tcaattaatg agatataatt tccgctttga ggaattgtat
    34201 caacaagacc ttgttgtcgg agttgaatca gtgcttcacg tattggtgta cgaccaatag
    34261 aaagtgtttc ttctaatttt ttttctgata ctttttttcc aggttctaag tcagcataga
    34321 taatttgtcg gcgaatagat tggtaagctt gattttggag actagtttta attgtcatac
    34381 acaatgctcc ttaactttta ttttaactga tattttaatt ctgttcatgc catataatat
    34441 caaattttga agtaatacac tagttataaa aataaaagaa tacatgaaaa aaagaaaaaa
    34501 taagtttcga taaaatcagt ccaaaaagaa ggagtatttt atttttctga ataaaaaata
    34561 caatcattct gatatggtag aaaaaagcaa gtttattccc tttttatatt gacaacggtt
    34621 acaatgggtt ttaaaatata aaatgtatca aaactaacat atcagtttga ggaggttaat
    34681 tatggtttcg attaaaaatt ggcaaaattc tatcgaagaa ttcaatgaaa tgcagattct
    34741 actgccgtgt tttgatgtca aagaagttaa gcaagaaggc cagcaacatc cagtatggct
    34801 tcattttggt ggaggaaatc tttatcgagg atttcatgca gaaattgctc aatgcttagc
    34861 agatcaaaat gaattagaaa caggagtagt tgtgtgtgaa acatttgatg aagaagtagt
    34921 ggaaaaagct tatcatgact ataacaatga cattttggaa attgttatga gtggagatgg
    34981 tgaactcaaa aaaagacttt tggcagtaac ggctgatagt ttatattgca atccgaagaa
    35041 tatagcggac tatcaaagag tggttaaata ttttgaagat cctaaattac agtttacaac
    35101 gtttactatt actgaaaaag gttacagttt aaaagattct aaagggcaat tcttttcggt
    35161 agtcaaagct gatatcaaat ctggacctag caatcctcaa catacgatga gtattgtaac
    35221 atccttgtta tatagcagat ttaatgctgg agagttgccg attgcgatgg tttctactga
    35281 taatttctca cagaatggtc aacgttttca agatagtatt tttacgattg cagaagcttg
    35341 gcaaaaagaa ggatatgttt ctgaaaaatt tattgcttat ttaaaggata aagaaaaagt
    35401 gacttttcct tggagtatga ttgatcgaat tacgccaaat ccttcagaga atgtaaagga
    35461 agatctattg atgatggggc tcgaggattt agatatcatt cacacaaaaa aacataccaa
    35521 tattgctgct ttcactaata ctgaggtagt tcattattta gttattgaag atagttttcc
    35581 aaatggccgg cctaaattgg aaaaggctgg tgtgattatg acagatagag agacggttga
    35641 taaagcagac atcatgaagg ttacgacaag cttgaatcca cttcatactg ctttggcagt
    35701 aacaggttgt ctgctaggat atcaatcaat ttcagcagag atgcaggatg atgatttggt
    35761 tggtttaatc aaaggaatag gttatttaga aggtttacca gtagttgaaa atccaggcat
    35821 tattgatcca aaacaattta ttgatgaagt aattaataaa cgtttgccta atccctatat
    35881 tccagataca ccacaacgaa ttgcaacaga tacttctcaa aaagtagcga ttcgctatgg
    35941 tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca gaaaatctaa catttattcc
    36001 tatcgctatt gctgcttggt tacgttattt actggcagtc gatgatcaag gggaaaattt
    36061 tgtgccaagt cctgatccgt tattggtaga gttgcaggat aaattatcag tattgaaatt
    36121 aggtgaacaa gatccttcaa agattcattg tgcggtacag gaaattttag aaaataaaga
    36181 aatctttggt ttagatttat atcaagttgg ggtaggaaag aaaactgaaa aaatcttttg
    36241 cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc aataaatata taacagaagt
    36301 tggaggaaag taactatgga aatgactttt agatggtatg gggcaaatga agaaaaaatc
    36361 tcactacaag aaattcgtca gatcccagca atgaaaggaa ttgttggaac attaatggat
    36421 ataccagccg gagaggtttg gccgagagaa cgaattaaat ctttaaaaga tgaagtcgaa
    36481 gccaatggac ttaccttgaa agttattgag agtgttaatg ttcatgatga tatcaaaatt
    36541 ggtttaccaa cacgagatca atacattgag aattataaac agacgattcg caatttggca
    36601 gaatttggga ttgaggttat ctgttataat tttatgccag tatttgattg ggtaaaatcg
    36661 gatttggatt atcgtttaga tgatgggtct tctacgttag cctatatttc aaaaaatatc
    36721 cctactgatc cggaggaaat tattagagaa gtagaaaatt ctactaataa ttttgaattg
    36781 ccgggttggg aacctcatcg gttatcacaa attaagcaat tattagaagc ctataaagaa
    36841 gttgatgaag agaaattacg agagaacttt gcatattttc tcaaagcaat tatcccgaca
    36901 tgtgaagaag ttgggattaa aatggcagtt cacccagatg atccaccata ttccattttc
    36961 ggattacctc gtattgttaa aaatcgtgac gatttagatt ggttgtgtaa tgtagtggat
    37021 tctccttcta atggtattac attatgtact ggaagtattg cagaacgacc agataatgat
    37081 gtctatgaaa ttctatctga attttgtcaa cgggatcgga ttccttttgc acatgttcgt
    37141 aatatcaaat atttaagtga caatggactc gatttttacg aagcgccgca taaatcagaa
    37201 tatggttctc tagatatgta taagatatta aaaacaatgt acgataatgg atttaatggc
    37261 tatattcgtc cagatcatgg tcggatgatt tggggagaaa ccggtagacc tggttatgga
    37321 ttatatgacc gagctctagg tgcagtttat ttaaatggta tttgggaagc gcttgaaaag
    37381 ggggcgagta aataattata taagggagtt tatttagagg aaaaattttc tcttctaaaa
    37441 actaacggaa ggaaaggagt gtttaaatat gtttttagat aagaactttt tattagagaa
    37501 tgaaatagct aaaagacttt ttcataaatt tgcaaaacac caaccaatta tcgactatca
    37561 ttgtcattta gacccagcat atttgctaga aaataagcaa tacaagaatt tgactcaggt
    37621 atggttaaat gataaaggag ctggagatca ttataaatgg cgattgatgc gagctaatgg
    37681 tatagaagaa gagttgatca gtggtccagg tgatgattat gaaaaatttt tagcttttgt
    37741 aaaaacagtg gagaaggcgc caggtaatcc tatttttgaa tggtctcatt tggaattaca
    37801 acgttacttt gatattgatt tgacaatttc tgagaagaat gcaccagaaa tttggcgacg
    37861 tgctaacgaa aaaattcaaa cggattccta tcgcccgaaa gaattgttgg agatgatgga
    37921 tgtgaaagcc atctgtacaa cggatgatcc tacagatgac ttgtggtatc atactgagct
    37981 ttctaaacag gataattctt tcaaggtatt acctactttc cgtccagata aggtctggag
    38041 tatcaaatca tcaggctttt cgaactatat tttaaaactt agtcaaacag cggctatacc
    38101 aatcaaagac tttaatgggt tgaagcaagc attgagccaa cgagtggatc attttcatca
    38161 aattggctgt cgattagcag atcacggttt gaatacttat ttttatgaaa tagcagatga
    38221 aaaaatactc gacaatctgt taataaaagc tttagcagga aaaacggatt tccatttatc
    38281 agaaattgca caatttgtta caggtattca attacatttg atgtctgagt atacaagata
    38341 tggttggacg atgcaaatgc atttaaatgc tttaagaaat aattcaagta tattaaagga
    38401 acaaattgga gtagatgtcg gtggtgattc gatgggggat caagctagtt tgacacaaaa
    38461 tgttgtagca ttgttcgata ccgctgaaaa gagtgggtgc ttaccaaaaa caattttata
    38521 ttcattaaat ccaactgatt ggcttccact agcaacagca atgcaaagct tccaaggggg
    38581 tatgattcaa aaaatccaat tgggatgtgc ttggtggttc aatgatacag ctgaaggaat
    38641 gcgtcaacag ttgactacga tggcacaaca aagtttgttg tcgaatttta caggtatgtt
    38701 gactgattct cgaagttttc taagctatcc tcgccatgag tattttcgtc gagtattatg
    38761 tcaattacta agcgagtggg ttaaagcagg ccgaattcca gatgatgaaa tgtatcttag
    38821 tcagatagtg gagaatattt gttatcgtaa tgcgtataat tattttaatt tttttgaaat
    38881 cggaagtaga aaataatact tgaaataaaa gcttaaattt attctttgta agttagtaca
    38941 gctagacaaa gtatgtatcc ataaaggaag gagatagata tgaaaaaagt agaaatttta
    39001 agtaagttag aagcggctgg agtcattgcg gttgtacgtg ggaaaaataa ggatgaggcc
    39061 attaaagcaa taaattcaat tattgcgggt ggaattaaag ggattgaact aacttttaca
    39121 gttcctaatg ctgaagaaat aatcaaagaa attgctaaaa aatatgctga tcaaaaagat
    39181 gtaataatcg gagccggtac ggttttagat gcgtttactg ctcgtatcgc aattatggct
    39241 ggggcagaat atatcgttag tccatctttt gatcgacaaa cagctgagat ttgtaattta
    39301 tatcaaattc cttatttacc aggttgtatg acaattactg agataaagga ggcacttact
    39361 gctggcgtag atatcattaa actattccca ggttctgctt ttggtccatc cattgtaaaa
    39421 gcctatcaag ctccattacc acacgtaaat ataatgccta ctggcggagt aagtatagag
    39481 aacttagaag attggtttga agtaggagtt gttgcagttg gtgtaggtgg gaatttattg
    39541 gcttctgttg aaaaaggtga ttttgaagaa gtaagtcgag tagctagtaa atatatagat
    39601 aagttttatg agataaagaa ttattaggag aaaaaacaaa taatactctg aaatgtaaaa
    39661 aataaatact gactctttac gcttcagagt atagtacgta ctttcatcag aatgaaaacg
    39721 cttaacaaca gaagggagag aattgtatgg aaagaaaatt tggtataaga gacaaaatag
    39781 gatatatgtt tggagatttt ggaaacgatt ttactttcat ttttgctagt tcttttttaa
    39841 tgatctttta tacaaaagta ttggggataa gcggagctgc cgttggaaca ttatttttag
    39901 tagcaagatt tatcgatgca gttactgatg taactatggg gcgtattgtt gatagatcga
    39961 aagcaactaa gcagggtaag tttaaaccgt ggattttaag aatgagtggt ccagtcgctt
    40021 tagcaagttt tttaatgtat caaacttcta tgatttcagc ttctatgagc gttaaggtag
    40081 tatacatgta tgttacgtat atattatggg ggagtatttt ttacacatct ataaatattc
    40141 cttatggatc aatggcatca gctattactt ctattacaga agaaagaaca atgctatcga
    40201 cattccgaaa tgttggagct actttagcaa gtttggtcat tggtgtatta acaccattat
    40261 ttatttatac aagagatgat tcaggcgctc aaatagttcg tggtggatca acttttacaa
    40321 ttgttgcggg aatattttcg atatgtgcac ttatttgtta tttaatatgt tataaactaa
    40381 ctactgaacg agtaagaatt gaccaatctc aatacaaagt ttcattgtta aaatctttaa
    40441 aagggttagt taaaaatagg gcattacttt ctataattgg tgcggctatt ttcttactcc
    40501 ttg
//
LOCUS       DAZKAO010000008        38921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000008 DAZKAO010000000
VERSION     DAZKAO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.008"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..2195)
                     /locus_tag="ACG8H8_000340"
     CDS             complement(114..2195)
                     /locus_tag="ACG8H8_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8985443.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2719..3078)
                     /locus_tag="ACG8H8_000341"
     CDS             complement(2719..3078)
                     /locus_tag="ACG8H8_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP8985444.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3108..3449)
                     /locus_tag="ACG8H8_000342"
     CDS             complement(3108..3449)
                     /locus_tag="ACG8H8_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985445.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3446..4120)
                     /locus_tag="ACG8H8_000343"
     CDS             complement(3446..4120)
                     /locus_tag="ACG8H8_000343"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8985446.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5173..6471)
                     /gene="asnS"
                     /locus_tag="ACG8H8_000344"
     CDS             complement(5173..6471)
                     /gene="asnS"
                     /locus_tag="ACG8H8_000344"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP8985447.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6511..7701)
                     /locus_tag="ACG8H8_000345"
     CDS             complement(6511..7701)
                     /locus_tag="ACG8H8_000345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8985448.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7722..8249)
                     /locus_tag="ACG8H8_000346"
     CDS             complement(7722..8249)
                     /locus_tag="ACG8H8_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP8985449.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8296..11085)
                     /locus_tag="ACG8H8_000347"
     CDS             complement(8296..11085)
                     /locus_tag="ACG8H8_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP8985450.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            11344..12645
                     /locus_tag="ACG8H8_000348"
     CDS             11344..12645
                     /locus_tag="ACG8H8_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8985451.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(12821..13852)
                     /gene="queA"
                     /locus_tag="ACG8H8_000349"
     CDS             complement(12821..13852)
                     /gene="queA"
                     /locus_tag="ACG8H8_000349"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP8985452.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            14045..15220
                     /locus_tag="ACG8H8_000350"
     CDS             14045..15220
                     /locus_tag="ACG8H8_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8985453.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            15224..15865
                     /locus_tag="ACG8H8_000351"
     CDS             15224..15865
                     /locus_tag="ACG8H8_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985454.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            15862..16779
                     /locus_tag="ACG8H8_000352"
     CDS             15862..16779
                     /locus_tag="ACG8H8_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8985455.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            16776..17447
                     /locus_tag="ACG8H8_000353"
     CDS             16776..17447
                     /locus_tag="ACG8H8_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985456.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(17988..18476)
                     /locus_tag="ACG8H8_000354"
     CDS             complement(17988..18476)
                     /locus_tag="ACG8H8_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP8985457.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(18677..18943)
                     /locus_tag="ACG8H8_000355"
     CDS             complement(18677..18943)
                     /locus_tag="ACG8H8_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP8985458.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(18943..19206)
                     /locus_tag="ACG8H8_000356"
     CDS             complement(18943..19206)
                     /locus_tag="ACG8H8_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8985459.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            19727..19867
                     /locus_tag="ACG8H8_000357"
                     /pseudo
     CDS             19727..19867
                     /locus_tag="ACG8H8_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(20009..21832)
                     /locus_tag="ACG8H8_000358"
     CDS             complement(20009..21832)
                     /locus_tag="ACG8H8_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8985460.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(22430..25135)
                     /locus_tag="ACG8H8_000359"
     CDS             complement(22430..25135)
                     /locus_tag="ACG8H8_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP8985461.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(25441..26388)
                     /locus_tag="ACG8H8_000360"
     CDS             complement(25441..26388)
                     /locus_tag="ACG8H8_000360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP8985462.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            26578..26847
                     /gene="rpsN"
                     /locus_tag="ACG8H8_000361"
     CDS             26578..26847
                     /gene="rpsN"
                     /locus_tag="ACG8H8_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP8985463.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(27023..27385)
                     /locus_tag="ACG8H8_000362"
     CDS             complement(27023..27385)
                     /locus_tag="ACG8H8_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP8985464.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(27396..28517)
                     /gene="alr"
                     /locus_tag="ACG8H8_000363"
     CDS             complement(27396..28517)
                     /gene="alr"
                     /locus_tag="ACG8H8_000363"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP8985465.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(28533..28883)
                     /gene="acpS"
                     /locus_tag="ACG8H8_000364"
     CDS             complement(28533..28883)
                     /gene="acpS"
                     /locus_tag="ACG8H8_000364"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP8985466.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(29013..30524)
                     /locus_tag="ACG8H8_000365"
     CDS             complement(29013..30524)
                     /locus_tag="ACG8H8_000365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8985467.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(30846..32198)
                     /gene="murF"
                     /locus_tag="ACG8H8_000366"
     CDS             complement(30846..32198)
                     /gene="murF"
                     /locus_tag="ACG8H8_000366"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP8985468.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(32351..33427)
                     /locus_tag="ACG8H8_000367"
     CDS             complement(32351..33427)
                     /locus_tag="ACG8H8_000367"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP8985469.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            33623..35368
                     /locus_tag="ACG8H8_000368"
     CDS             33623..35368
                     /locus_tag="ACG8H8_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP8985470.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(35648..36085)
                     /locus_tag="ACG8H8_000369"
     CDS             complement(35648..36085)
                     /locus_tag="ACG8H8_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP8985471.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(36069..37442)
                     /locus_tag="ACG8H8_000370"
     CDS             complement(36069..37442)
                     /locus_tag="ACG8H8_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985472.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(37764..38252)
                     /locus_tag="ACG8H8_000371"
     CDS             complement(37764..38252)
                     /locus_tag="ACG8H8_000371"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985473.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(38274..38876)
                     /locus_tag="ACG8H8_000372"
     CDS             complement(38274..38876)
                     /locus_tag="ACG8H8_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8985474.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        11084 a         8327 c         7072 g        12438 t
ORIGIN      
        1 tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct ttacattttt
       61 cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttcat tgatcaacgt
      121 ttatttttta catgcaagct tcgcatggca ttcaaaacag ctaatactgt caccccaaca
      181 tctgcaaata ctgcatctcc cattgatgca aaaccaaaag cacctaaggc aagtaccaaa
      241 atttttatcc caatagcaaa gacaatgttc tgtttgacga ttttcaatgt tttctttgct
      301 aaatggattg cttctgcaat cttcgatggt tgatcattca tgatgactac atctgctgct
      361 tcaattgcag catcgcttcc caatccaccc attgcgattc ctacatctgc tcttgctagc
      421 accggtgcat cgttgattcc atctccgaca aatgcggtct ttttattgtt tctttgtaag
      481 atttcctcta accgctgcac tttatcttct ggaagcaatt cgctatatat cttgtccacg
      541 cctacttgct tcccgatatg atcagcaatt tttttcgaat cgcccgtcaa catgactgtg
      601 ttcttcacac cagccttttt taattgaacc aatgcgtcta cggcatcttc tttcaaggta
      661 tctgcaataa caagataacc actgtaactt tgatcgatcg ctagataaag caatgtaccg
      721 atttcttgac ttgaaggaaa agagatttca aatctttcca tcaatttgtg attacctgct
      781 aatacttgtt tgccatcaac ttcagctaag atcccttcac ctgcaaattc ttggatatta
      841 gctgttggta aaagcggacc tgcgtagcct tctaaaacag attgagcaat cggatgtgta
      901 gaaaactgtt ctgcactcgc aacatattgc atgaatatct ttggatcgat ttttgtatcg
      961 atcgtctgga tcgaaaagtc acctttcgtt aatgtgccgg ttttatcgaa taccactgtc
     1021 tcagtttgtg caagcaactc caagtaattt gaacctttta ctaatacacc gattttactt
     1081 gctcctccga taccgccaaa gaaactaagg ggtacggaga tcactaatgc acatggacat
     1141 gagatcacta aaaatgtcaa tgctcgatag atccattcag aaaacgccgc tccagaaaca
     1201 acaagtggag gtaaaacagc taataaaacg gccaatccta caacgactgg tgtgtaatag
     1261 cgcgcgaatt tagtaatgaa attttcagct ggtgcttttt tactgctggc attttctact
     1321 aattctaata atttgctgat agtagaattt tcataagatt tttctacttg aactaaaaat
     1381 ggtttatttt tattgataaa tccacttaat actgtttcac caacatatac agatttaggg
     1441 actgattctc ctgtaagtgc tgacgtatcg actaatgatt ctccttcgat gatcgttcca
     1501 tctaacggta cacgttcacc aggcttcaca agtacaaact gaccgatctg cacgtcttca
     1561 gggttcacgg tcatcaatcc ttccgtcgtc tgcacgtgag ctgtttctgg acggatcgcc
     1621 atcaattcgc gaatcgattt tcttgaatga ctgactgcca atccttggaa atactcgccg
     1681 atttgataaa atagcatcac tgctacggct tctggatatt caccgatcaa catagcgcca
     1741 atcgtagcaa cagacataag aaaattctca tcaaagattt ttcctttgac gatattcatc
     1801 actgccttct tcactacatc atatcctatc agcagataaa tcaataaata ccctatcaag
     1861 cgaattggtt cattaaaatc aaataatagc aataaagcga tacctgcaag ggaaaataaa
     1921 atccggaaca gcgtatttct ctctttttca tttccatgtt cagatacttt ttccttatct
     1981 atttctgtta ctttgacatc tggttctaat tccttcacta ctttttttat ttcttgttca
     2041 atcgtagcca aatgatcttc ctctgcgtca atcgttagtt ttccagaagt gaagtttacc
     2101 gttgcttcct tgactccatt gatcttctga actccttttt cgattttcat tgcacagttc
     2161 gcacaatcta aaccttctaa tctgtaactt ttcattttaa tgacctcctc gcctcaacat
     2221 atgaatatct cttcataact aattataaat gaacggttat tcatatgtca aggtatccga
     2281 aagaaaaaat gaaaaacatc tgcatcggaa aatttcttcc atacagatgt tttggcttat
     2341 tctttctttt tcttttcgat cattgccact gctcttatcc aacccgcact tgccttcttg
     2401 atcttgacag gatagcaact aagtttgaag cctgttgctg gcaatttgtc taaattcatt
     2461 aatttttcga taggacaata tcttttttca atccctgcaa aatgtccttc ccagatattc
     2521 gaagctccac tacgctttca aaaatataga aagagttctt taatgaaaat tatttcaact
     2581 ttccataaac agtaacccca tataaataaa agacgtaaaa gtcacatgga agaacttata
     2641 caggagaacg catagagtca ggtgaaatac atgtatccca cctgactcta tgctttatct
     2701 aacctttatt attctctttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc
     2761 aagtttgaaa gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc
     2821 gctgacgaca tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac
     2881 aatcaatgac atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag
     2941 ggaggcgatc ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag
     3001 tatccccgct gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac
     3061 atttcctaaa caactcattg tatttctcct tctattactg tttttgatta aaactgggca
     3121 aaaaatgatt ttattcttct ttttttagct tccagtatca aatattctgc aaattttttt
     3181 tggatttctt ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt
     3241 cgctgatcca atcgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt
     3301 ttttcttttg ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt
     3361 tttgctttta cgaatgattc tggatgatca gtaatcagct gattgatttg aggtagatct
     3421 tcttttactg cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc
     3481 cactaaaaaa atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc
     3541 cgccaatcgt tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taaacaatcc
     3601 ctaaagcatt gaatatacca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt
     3661 tacgaacaat ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta
     3721 taggaagtac aaacggctcc atcaagatga tgttgcagat tcccactgtt tgaagttcat
     3781 ttagtaattt tcgataaacg gattcaaatt gctgagcaga tttctcagtt ccgaatatag
     3841 cctgatcgct agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag
     3901 gttgtaaagc taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc
     3961 ctatcccacg gttcatgcat tcaaattcca tcgttggata ttgggagatt agttttccgg
     4021 caatcaaaag tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt
     4081 ctgtaatgct atcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg
     4141 aataaaacga tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa
     4201 aatatatgta taaaaaatga caccagccta gtcccttcaa acaattgaat gaacgctcat
     4261 caagtgtcta gttttttatg ctattttttt cttaacgtgc cggattcccc ttgattcctt
     4321 cgttgtttgg tccagagaga tcgatcacac ctaagcttcc gtcaatcaga ccactgatga
     4381 cgccatcaga acatatattt ataggctcat ctaagacttc tagtaaatgt ccctttacta
     4441 atatctcttc tagattgctt gggatagatt ggtcataact ttatctggtc cgatgatttc
     4501 aagggtgaat tgaacttaca tatggattat tgcatgctga aaatacggac ctactccatc
     4561 ttcgaatgtt actattagtt tgttttcagg tgctagatgc tgctttattt caacagcaga
     4621 agcatcatca aatcagattt ccatttgaat taccttcttt gtaaatgttt gattgattgc
     4681 gatttcgtta tagaaccttg tgaatcaaat agcaatttct tttgctttct tctcttgtct
     4741 gctggaaact tcctttatcc cgtattttaa aaaaagaact gcgagagttc gcagttcttt
     4801 tttagaataa acggtgctct ccggccaact cctcggtcac aagtcacaat agtcatcaaa
     4861 catgaaaaac tgttttttga aattactcct tgtgactcgg agctgaacgg caggcgcaca
     4921 acctcttcct taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     4981 aaatgtggaa agccatttcc acaaaatttt tcttaatact cgaaactaaa cagctttttc
     5041 acaacctctc cactaaacgg tattcaaaag cttacatctg cggtaataag tcccgccaat
     5101 tcaaaaacat gaacagctgt ttttgcttgg ctgttctaat tacttgatgc agaacagctt
     5161 tttcatcacc tcttatggat aaattcggtt caacaaacgt gggaatggaa tagcttcacg
     5221 tacatggtcg ataccagcaa tccaagtgac tgttcgttca agacctagac caaaaccaga
     5281 gtgcggaaca gaaccgtatt tacgtagatc aagataccat gaatattctt tttcatccaa
     5341 accgtgatta cggatttgtt ctagcaagaa atcatagtct gttgcccgtt cgcttccgcc
     5401 aataatttca ccgtaacctt ctggtgcgat catatctgca cagatcacca cgtcttctct
     5461 tgttggatgt ggtttcatgt agaatggctt gatggctttt ggatagttta ggataaagac
     5521 aggctgttcg aatgaattcg caatgaacgt ttcatgaggt gaaccaaagt cttcgcccca
     5581 ttcgatgtca tcaaatccat ttttcttcag taattcgatc gcatcatcat aagtaatacg
     5641 agggaatggc aattttgtat aagtttttaa gacttctttg tctcgtccta gtacatctaa
     5701 tgcatattcg caatgatcta aaacattttg tactaagaat gctacatatt gttcctgtac
     5761 ttctaaactt tcttcttgat gagtgaaagc catttctggt tcgatcatcc agaattcgat
     5821 caaatgacgt cgtgtttttg atttttcagc acggaaagtc ggaccgaatg aaaagacttt
     5881 tccgaaagcc atagcagcag cttccatgta taattggcca ctttgagata aataagcttt
     5941 ttgatcaaag taatttgttt cgaatagatc cgtagtgcct tctggtgcag aaccagtcaa
     6001 gatcggcgga tcaattttga caaaatgatg ttgattaaag aattcatacg ttgcacgaat
     6061 gatttcattt ctgatctgca taatcgcatg ttgtcttgaa gaacgcagcc ataaatgacg
     6121 atgatccatc aagaagtctg ttccgtgttc ttttggtgtg atcggataat cgtggctctc
     6181 gcttaccact tccacatttg taattccgat ttcataacca aattttgaac gactgtcttc
     6241 acggatttct ccagttacta acacggatgt ttcttggtgt agatttttag ctgtttggaa
     6301 aacttcttcc gacacttcgc tttttacaac tacaccttgg aaaaaggctg ttccgtctct
     6361 taattgtaaa aatgcgattt ttccgcttga tcgcttattt gcgacccaag cgccgattgt
     6421 tactgtttct cccacatggt ttttcgaatc gatgatttga atttgttcca cgtatgactc
     6481 tccttttata ttccttgaat ttctttagga ttaattttga cttttactct caataaattg
     6541 cttgattctg cgtaccgctt cttctaacgt agcgcgatca gtagcataac tcatgcggac
     6601 attttctggt gccccaaagc cttcgccagt cacaagcgct acccctgttt cttctaaaag
     6661 atcttctacc cattgtgtga cattttcata tttacacatg cgtagcgttt cttttacatt
     6721 aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa acacccggca gttctgccac
     6781 aagcgaatat aacgtattca ggcgttcttc aaaggcgttc cgcatttctt ccactgtatc
     6841 ttgctcgcct ttcaatgctt caacagctgc atattgactg actgctgtcg gattactggt
     6901 tgactgggaa gcaacagcga tcattccatt gatgatcaca ggatttccca ccgcataacc
     6961 aatgcgccag cctgtcatcg catatgtttt agacacgccg ttgatgatga tagtctgatt
     7021 tttgattgct tctgaaatcg ttgcaattgg agtaaactca ttgccattat aaactaaacg
     7081 accataaata tcatcagcaa ctatcaaaat atcatttttt actgcccatt caccgattgc
     7141 ctgcagttca ttcttgctat aaatcattcc agtcggattc gatggagaat tgatgattac
     7201 ggcttttgtt ttttctgaac gtgcttgttc tagctgttca acagtaactt tccagctatt
     7261 ggattcttcc cctttcacaa agacaggaag tccttctgcc agttttactt gctcaccgta
     7321 actgacccag taaggcacag gaatgatgac ttcatcttgc gggtcaagga tcgtttggaa
     7381 aagggtatac aaagcaaatt tcgctccgtc tgttacgact gtttgtgaag gttcatagac
     7441 aagtccatag tttttcttca gataatcaac gattgcctgt cgaagctctg ggattcctgc
     7501 tgttggtgta taatagctag ctttcccatt gcggattgct tcgattgctg cttcttggat
     7561 gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa ctcagcacat ctctgccttt
     7621 tgcttttaat gccttggcct ttgccgcagc tgccaaagtg acagacggtt ctaatcgttt
     7681 tgttcttttt gataactcca tcccattcat tcctttacct tttaaattcc cttgattgtc
     7741 ttgacttcat ctccagattc aaagccaatc aggtaataat tcagtttccc atttgtttct
     7801 tttcctacta cttcccacac aggctgatca tcatacatcc ctaaagcagc tttttcgatt
     7861 tggatttcag gatgggccgc agcaattgtc tgttttgctt cgttttcagt cagtcccttt
     7921 gtttgatcca atacttttac tttttcacca gatttaggaa tgataacggc gatttctttt
     7981 cctttatcat ttgtacctgt cagtgaaaag tatgtcttct ttcgagtaaa ccaataaaaa
     8041 tgatcggttt cctcaagatt tgcatatttc tttgcgattt ctactgcttc tttcttcgcc
     8101 tgcaccgccg gacgactgga acgcaagtaa aaaatcgagc tgatcacgat gaccgctaat
     8161 aaaaatacga tcaagccaag taacacgcga ttaatcgttt tttcctttgt ctgttttttc
     8221 ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt atagcagatt ttaattatga
     8281 aaaaagacag ctcctctaac ttccttcttc atttttcttt aagaattcat ggatatcctg
     8341 atagatttcc tccatagcta gctctttgat cggtactttt ttcggtaatg catggatgat
     8401 ccgtgcgcca tattttgcag tgactagtct tcgatccaaa ataagcaata cgccacgatc
     8461 tgtctccgtc cttatcaagc gacctaatgc ttggcgtaac ttcaaagctg ctttaggtac
     8521 ggattcctgg taaaaaggat tgatcccctc tgattctaaa taagcgttcc tagctttgac
     8581 catcgggcga agcggattat cgaagggtaa acgtgtgact acaacgatct gcaaagcatc
     8641 tccaggcaaa tctacacctt cccaaaagct gtcagcgcca aacaatacgc ttttttctga
     8701 caaaatgaag cgtttcaaaa gcttctcccg gcttccacca aacccttgag ctagtatttc
     8761 tcgtcctttt tctagcatag acagatgcat tttctgatag accttctgca aaacatcatg
     8821 agaagtgaat aacacaagta ttggccgatc ttcttgttcg atcagacctg taagtgctgt
     8881 ctctaaataa tcgatatatg cttgattgct gacttgatgg acggcaggtg cctcttttgg
     8941 cacatagatc cttgcctgtt tctcataatc ataaggagag gcaatcactt taagaggtgc
     9001 tgcttcaatg ccaagcctct tagagagata gtgtcgatta gggccgatct tcagcgttcc
     9061 tccaatataa agaatacgat catatcgagg ataccaaaca gtcgacggca acatagccgc
     9121 ttgaaaatca tggagatgaa aaatcatctg ctgcgctgtg ttcccaggaa cgaaccaatg
     9181 gatcaaatga tctcgccagt cttccagcca ttgatccatc agcaagtatt gcttgtctaa
     9241 ttgatcaaat aggctatata aacttgctaa atcgatccgc atctggtttg tccagctttc
     9301 ttggataatc tcaaaagtca gacgcatttg tgactgtagc tgctggatct cctgataaaa
     9361 aagctgcagg tgatcaatgg ctgtcccaga ttccagtggc agctgatcca acatctcttt
     9421 tgtcatcatg acttcttctg gatactgtcc gttaggttgc ttgccgctca ctaaactcat
     9481 taatccttct gccatacaaa tctgtgcttc gcatagcgca gaaagttctt ctcgataaat
     9541 cgataaccag cgtaaggctt catgttcttt cggtaaatag ctttgtattc ggtcaaacag
     9601 ctgatttggt tcttctaatt gctggatttg tttatggaaa tagttaatac ttaatcgttt
     9661 gtcatttact ttttcagcga tttccggcaa atgatgtact tcatcgatca acaaaaatgg
     9721 actagctgga agcaatggtt cttttcgctg tgtttcttgt actaaaaacg catgattgac
     9781 aatcaaaaaa ttactttgcc tgattttctt ttgcaagaaa cgaacaaagt cttgttcgta
     9841 aaatgactga tttcttgcta aaaatccaat acctctgtgc gcgatatctg caaagaatga
     9901 atggttcaaa tttgttaaat gtaattcatc caaatcacca gtcttcgttt gcgtcagcca
     9961 aactaagacc gccatttgat acaaagcata ttgtttttgt tctggcggtt ccacaagtgt
    10021 gcctttaaaa cgctgcaaat caatatagtg gcgatagctt ttgataatta caggttgtat
    10081 gggctgatct agcaattgat tcagcaatgg aatgtctttt tctatgatct gctgctgcaa
    10141 aactagcgaa acggtactga tgacagcggg tttttcaggt gtagccaaat aactcaatgg
    10201 cagcagataa ccgattgttt tacccattcc tgtagctgct tcgatcatca aatcttttga
    10261 ttcatctttt gtgaaatggt cgtagaccgc attcatcagt ctgttctgtt cttttcgata
    10321 catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt tttcttggat atgtccgctc
    10381 gccataatgg acggatgtga atagttccac ttcttttttc tgcaaagcta gcccatcgac
    10441 gatttctaat gagggatcaa gtggctctgg tttttctttc atttcttcaa tcacatgata
    10501 aatgaaacga ctcgtatcca tacctgtgtg tctacttaat cgcgcaatct gctctaacgt
    10561 gatgattggc aattttctca tccgttcttc gatcagcaaa agaagttgac ctgtcacttc
    10621 ggcatcacta tctgcctgat gaggattctc atgaataaat ccaaggcttt ctgacaaatc
    10681 agctaagcga aagcttggtt ccgtcggcaa gaaaatttgt gcaagttcta ctgtatcgat
    10741 gcctggtatc ttcaaactag gaagtccgca tcgcatgagt tcatggttca aaaaattata
    10801 atcaaaataa atattatgcg cgacaaatat ggtgtctgcc aaaagattgt aaatcgtttg
    10861 ggcgacatct tcgaaataag gcgctttttg gacgcgttga ttcgtgatcc ctgttaaatg
    10921 ctggatttgt tttgaaatct tccgccctgg atgaatatcg atcgaaaaac gtgaagtgat
    10981 ccgacctgat tcaatcatga cgcagccaaa ttggatgatc cgatcttctt ttggattcgt
    11041 cccagtcgtt tcgatatcaa cgactgcata agttcttggt ttcaaaatat cttcctcttc
    11101 tcctgcctct aatgttcttt tgaaattata ctacaaagtg cagaaaaata ctctcatcgc
    11161 tttttaaaga aaatagacaa aaacgcccag acacgcaaat gtctgggcgt ttatttttgg
    11221 ctctataatt tctaggactg atagaaaatc attctatcgg tcccttttga gcatgaaaaa
    11281 acaaaacata cagaagctcc gttagaattg aatcaccaca acacaatcaa aggagcgttc
    11341 tgtatgcctg atcttcatca tatcaaacta ctacttggaa tcaaagataa aaacattttc
    11401 atcacaaatg tggaatcgaa gagcattaag aatatcaaga gtcttgttgt ttctgcgacg
    11461 ttaagtaaag aaattcgtcg ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc
    11521 gttaagaacg gaaagaaaaa atcgctcatt caacttaata aatgtgcgaa ccaactgact
    11581 tatcttgctt tagcgaaaca acgctaccac tgtcgtggct gccacaccta ttttacggcg
    11641 aatacctaca ttgtcgatag aaactgcttt atcgctaaac aagtgcgcta taaaatttta
    11701 gaagagctaa ctgaaaaaca agcaatgacg acaattgcta agcattgcgg cgtttcttgg
    11761 tcgaccgttt ctcggacact cgcttctctg atccccatga caaaagtaaa aagaaattgg
    11821 ttaccgcgtt gtttattaat ggatgaattt cgttcactga aaaatcaagt cggtccctac
    11881 tccttttctt gtatggatgg cgatactgga aaattactgg atattcttcc ttcacggaag
    11941 aaaaaggctc tcgtttctta ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag
    12001 gtcatcgtga cggacatgaa tgtttcttat gcctctctga taaaggaatg ttttccaaag
    12061 gcaaaactcg ttgtggatcg ttttcacatt gtcaaacatc tcattcgtaa atttgaggat
    12121 attcgcgtac gaatcatgaa aagctttgat cgaaacgatc caacacaagc caaatattat
    12181 cgacaattaa aggcgttgtc tcggcttctg atcaaacgac aggacaagct tgtttatgac
    12241 aaatggacca aatggcgcaa ttttggttgg gcgtatctca ccgaaagtga agtggtggat
    12301 cgtctgcttt cgatctctga cgaattacga atcgcctatt tttactatca agaaattctt
    12361 caagcgtttc acgacaaaga ggcggacact ttctttaaac ttgtaagaac gatgcccaat
    12421 tccgttccaa aagaactgca ttctataaaa aaagctttta taaactatga atccggcatt
    12481 cgtttagccc tcgaattgcc ttattcaaat gccaaaatag aaaatctgca tacccacatc
    12541 aaagcattga aacgagtcgc ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt
    12601 ttcttactaa ataatctgat tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt
    12661 ctaccttaga tgttcttatc tcactctatc agtcctattt gacaaagagc caagaatcaa
    12721 ttctgattca tcagtcccat aaattataga gccctttttc tttgtcataa aatctttgta
    12781 aagctttttt gttattcaaa actagcggct tttcataacc ttatttcacg aacatcgcat
    12841 ctccaaaact aaagaaacga tagcgctctt caatcgcatg ctggtaagca gataaagtca
    12901 attctcttcc tgcaaaggca ctaactaaca tgactaaagt cgattttggc aaatggaaat
    12961 tagttgagaa agcttctacc actttgaatt catagccagg cgtaatgaaa atatcagtcc
    13021 atccgctgtc tgctttgatt tctccattaa atttcgttcc aatcgtttct aatgttcgaa
    13081 ttgaagttgt tccgacagcc acgattttcc cgccattttt acgtacttgg ttcaatcgct
    13141 cagccgcttc ttccgtcaaa cggtagaatt cactatgcat atggtgttct tcaattttat
    13201 ctacgctgac aggtcggaat gtacctaacc ctacatgtag tgtcagatag actaattcaa
    13261 caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg
    13321 ctgcagcaga accattttct ttcgcataga cagtttgata gcgatctggg tcttccagac
    13381 gttctttgat atatggaggc aatggcattt ctcccaatga ttcaagaatc tctagaaaaa
    13441 ttccttcata ctcaaagtca atgatccgtc cgccgtgttc caattcttct ttcactactg
    13501 cttgcaatct gccatcacca aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt
    13561 tgatcaatgt ctcccacgta tctccttgtg tattcgttag aagaaggact tctaaatgcg
    13621 caccagtatc tggtttttct ccatataaac gagcaggcag aactcgtgta ttattcataa
    13681 ctagcgcatc accaggctgt aactcttcaa tgatttcatg gaaatgttta tcttccatct
    13741 ctccagtttt ccgatccaaa acaagtaaac gagaatgatc gcggtctttt aatggtgttt
    13801 gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc tgttgttaac atatcgtcac
    13861 tcactttcta tcatgtgctt catcattcta tcatgttttt cgttctgttg gcaaaatcag
    13921 cactttttca cttctatttt ttgttttcct aaataaaaaa actaccctat ttgtattttt
    13981 tcattgtttt aggcatacta gtaatagata gttcttcatt gaaaatagaa aaagaggtgg
    14041 caccatgatt gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga
    14101 ggacgtcaat ctttcatttg aaaagggtga gttcatttgt tttatcggta cgagcggaag
    14161 cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact gatccaacac aagggaaaat
    14221 tttgattgat ggaaaatcca tccaagaaat tgatccagtg gaattgcgac gtcagatcgg
    14281 ctatgttatc caaagtattg gattgatgcc tcacatgacg atccgtgaaa atatcactct
    14341 tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat aaaacagctg aaaaaatgat
    14401 tgatcttgtg gaattgcccc gtgagatgtt agatcgctac ccacatgaat tatctggtgg
    14461 tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat
    14521 ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag accttgtcaa
    14581 agatttacaa gagcgtttgg gaaaaacgat cgtatttgta acccatgaca tggatgaagc
    14641 gattaaacta gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac
    14701 accagaaaat attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga
    14761 tcgcctacta caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc
    14821 aatcacgatt acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg
    14881 tgtagatacg ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga
    14941 aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt
    15001 tttttacgta aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg
    15061 gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc
    15121 atcattagtg gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt
    15181 tgcagagtca gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca
    15241 tcagcacggt tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc
    15301 tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa
    15361 agctgccacg attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt
    15421 attggcattg atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt
    15481 tatttattca ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg
    15541 gaattatcgg gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt
    15601 tgaattgccc attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt
    15661 gattgcatgg gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt
    15721 tagtggactg aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat
    15781 tttagcacta ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc
    15841 acttagagag gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct
    15901 cttcttatac ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc
    15961 atttcgatca caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga
    16021 atggttgaac attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact
    16081 atcaaccatc aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga
    16141 acagatttaa cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct
    16201 accgtgaaag atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt
    16261 gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa
    16321 gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc
    16381 aatcgtaaag gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg
    16441 atattaccta tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt
    16501 gttcttggct actctacgga tggacggatc gccagttatg atctagtcat gttggaagat
    16561 gatcgtcaat tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct
    16621 gatacgcctc atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa
    16681 atgcaggaat tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa
    16741 cgtttcttac aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg
    16801 tctttcaaca atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta
    16861 tccgtcactt cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg
    16921 ttgggatcat gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata
    16981 tcatccagac gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg
    17041 gagtcaatgt cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata
    17101 cgtataccgg gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga
    17161 tgactgctag acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg
    17221 caggcataag aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg
    17281 gtgctggtgg tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga
    17341 ttattttagc aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag
    17401 gtattttgga aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa
    17461 aacaaaagct tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta
    17521 aggcaataat ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta
    17581 tttatccatt ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg
    17641 actaagtttt tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca
    17701 tggaattcat accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca
    17761 attcttacat cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc
    17821 gatgccttca gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt
    17881 ttatacaatc gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag
    17941 cttctcgcca tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat
    18001 ccgtcgcaac tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc
    18061 aattgatcga tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt
    18121 gatgcattgc cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc
    18181 gtttgagtga cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg
    18241 acactggtat tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct
    18301 gggaaccaat aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct
    18361 gtttctcgat aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc
    18421 gttgccactg ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt
    18481 cttcctcctt gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc
    18541 ttcaaaggtg gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg
    18601 gttttagaac aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag
    18661 gttggctctt ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat
    18721 tttcctcatc aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc
    18781 cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca
    18841 atgtctgaat cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat
    18901 ccactttcgc agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc
    18961 aatatcatcg atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa
    19021 agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat
    19081 caatacagca ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt
    19141 tacctctttc aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt
    19201 agccattgtc tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa
    19261 aaaaatacaa taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa
    19321 gacccctttt tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg
    19381 tagccaaacg taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag
    19441 cctgtgagtc tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat
    19501 tgatatcttg aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga
    19561 ttttggccgt caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt
    19621 catgttcact atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa
    19681 aatggcactg agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta
    19741 tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg
    19801 tacattattt ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta
    19861 cagctaaata ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag
    19921 agggcacctt tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga
    19981 gggtctgttt ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga
    20041 caacgatatt ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga
    20101 gaatattctg ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt
    20161 tttgtttcgc tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg
    20221 agcgataaga agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg
    20281 cttcgatttc tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat
    20341 aatcaccgat cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga
    20401 ctaatacaag aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa
    20461 gctgttctgc tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg
    20521 gcataatgat gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga
    20581 ttgcatatga gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc
    20641 cttctttttt ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc
    20701 cgatagagta taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga
    20761 tcgaacctag cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt
    20821 gatacatgtg aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag
    20881 aaaaaccggt gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat
    20941 acaaaatacc atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt
    21001 caggaacgat cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga
    21061 tcaatgccat catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg
    21121 gtacagcatt actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc
    21181 gtaaaatcaa agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca
    21241 atggcacagc acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa
    21301 ggataaatgt gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat
    21361 tcagcatcat cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg
    21421 ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca
    21481 tgtagtcgat caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac
    21541 tgctgacgac atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata
    21601 gcgttaatga aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg
    21661 ctgcagcaga caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg
    21721 ataacgcatc agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat
    21781 tttcagcaga ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa
    21841 ctcctttaca cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg
    21901 ttatagtacg ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa
    21961 cagaaaaata tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt
    22021 atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt
    22081 ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca
    22141 ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag
    22201 cttgcatctg cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg
    22261 tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa
    22321 tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat
    22381 ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa
    22441 tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga
    22501 ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg
    22561 tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa
    22621 atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc
    22681 cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat
    22741 cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa
    22801 atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg
    22861 taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt
    22921 gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa
    22981 cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag
    23041 ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa
    23101 agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga
    23161 cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg
    23221 gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt
    23281 tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt
    23341 ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata
    23401 ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat
    23461 tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg
    23521 ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt
    23581 ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag
    23641 aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat
    23701 taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa
    23761 cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat
    23821 ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca
    23881 ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt
    23941 tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg
    24001 ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg
    24061 taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt
    24121 ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat
    24181 cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg
    24241 tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga
    24301 tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca
    24361 ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca
    24421 cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga
    24481 ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat
    24541 ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata
    24601 ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg
    24661 aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat
    24721 attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat
    24781 cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt
    24841 cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt
    24901 gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag
    24961 caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg
    25021 catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga
    25081 aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc
    25141 tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc
    25201 ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct
    25261 cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga
    25321 aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat
    25381 tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc
    25441 ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg
    25501 tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc
    25561 tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct
    25621 tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc
    25681 atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat
    25741 acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt
    25801 tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt
    25861 actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga
    25921 cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa
    25981 atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca
    26041 actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta
    26101 agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga
    26161 accaatctct gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt
    26221 tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc
    26281 ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc
    26341 aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc
    26401 cctttttctt taagtttacg tttatgtctt tattttgaca aaaaaaatgt cttttcgttt
    26461 tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat
    26521 ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg
    26581 gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct
    26641 gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcataa acttccttta
    26701 gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac
    26761 atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt
    26821 ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc
    26881 gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct
    26941 ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg
    27001 ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac
    27061 taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt
    27121 ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt
    27181 ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt
    27241 ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa
    27301 taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa
    27361 aataaatatc tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc
    27421 cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc
    27481 tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga
    27541 aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag
    27601 tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa
    27661 ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg
    27721 attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct
    27781 ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct
    27841 actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg
    27901 ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct
    27961 ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa
    28021 ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca
    28081 ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc
    28141 gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct
    28201 gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc
    28261 aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt
    28321 gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca
    28381 tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca
    28441 acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg
    28501 tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa
    28561 gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc
    28621 acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg
    28681 atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat
    28741 tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt
    28801 cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca
    28861 tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag aacactctca
    28921 ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt
    28981 tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat
    29041 ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta
    29101 taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt
    29161 ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct
    29221 ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt
    29281 gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta
    29341 tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct
    29401 ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt
    29461 tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct
    29521 tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata
    29581 tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc
    29641 gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt
    29701 tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc
    29761 aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca
    29821 tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga
    29881 tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg
    29941 aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt
    30001 ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac
    30061 atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg
    30121 ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag
    30181 ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg
    30241 tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg
    30301 ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca
    30361 aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat
    30421 gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact
    30481 gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat
    30541 ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta
    30601 ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca
    30661 ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt
    30721 tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt
    30781 ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca
    30841 gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg
    30901 ccttttagca taatactatc tgttgctgca agattttgtt tgatagcatc aatcaattta
    30961 ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct
    31021 tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata
    31081 tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct
    31141 aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc
    31201 atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt
    31261 agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt
    31321 atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa
    31381 cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt
    31441 ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt
    31501 tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat
    31561 ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac
    31621 ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc
    31681 ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt
    31741 tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt
    31801 ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg
    31861 gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa
    31921 taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt
    31981 tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct
    32041 tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg
    32101 gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg
    32161 cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt
    32221 tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt
    32281 tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct
    32341 ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc
    32401 ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga
    32461 gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt
    32521 catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt
    32581 aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa
    32641 tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt
    32701 ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga
    32761 tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg
    32821 ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca
    32881 tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt
    32941 ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac
    33001 cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata
    33061 cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt
    33121 ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg
    33181 gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat
    33241 gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt
    33301 cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg
    33361 aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa
    33421 tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag
    33481 cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa
    33541 cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg
    33601 ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat
    33661 cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga
    33721 acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct
    33781 ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga
    33841 tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct
    33901 ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg
    33961 gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc
    34021 taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat
    34081 caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg
    34141 ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca
    34201 aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa
    34261 aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga
    34321 aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc
    34381 aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga
    34441 tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag
    34501 ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac
    34561 ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg
    34621 gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa
    34681 agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga
    34741 tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat
    34801 tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa
    34861 tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt
    34921 gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca
    34981 ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt
    35041 ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg
    35101 gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag
    35161 gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg
    35221 gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg
    35281 gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat
    35341 ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa
    35401 gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt
    35461 tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac
    35521 ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc
    35581 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    35641 gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg
    35701 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    35761 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    35821 ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    35881 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    35941 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    36001 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    36061 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    36121 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    36181 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    36241 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    36301 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt
    36361 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    36421 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    36481 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    36541 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    36601 tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    36661 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    36721 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    36781 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    36841 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    36901 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    36961 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    37021 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    37081 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    37141 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    37201 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    37261 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    37321 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    37381 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    37441 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    37501 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata
    37561 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    37621 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    37681 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    37741 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    37801 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    37861 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    37921 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    37981 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    38041 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    38101 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    38161 ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc
    38221 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    38281 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat
    38341 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    38401 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    38461 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    38521 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    38581 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    38641 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    38701 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    38761 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    38821 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    38881 attccccaca tcttttttca tttattaaaa ctgcctcctt a
//
LOCUS       DAZKAO010000009        36948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000009 DAZKAO010000000
VERSION     DAZKAO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.009"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8H8_000373"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8H8_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP8985475.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="ACG8H8_000374"
     CDS             complement(1692..2711)
                     /locus_tag="ACG8H8_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985476.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="ACG8H8_000375"
     CDS             complement(2733..3740)
                     /locus_tag="ACG8H8_000375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8985477.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8H8_000376"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8H8_000376"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP8985478.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="ACG8H8_000377"
     CDS             complement(4391..6685)
                     /locus_tag="ACG8H8_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP8985479.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="ACG8H8_000378"
     CDS             6945..9110
                     /locus_tag="ACG8H8_000378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP8985480.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="ACG8H8_000379"
     CDS             9414..10247
                     /locus_tag="ACG8H8_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP8985481.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="ACG8H8_000380"
     CDS             complement(10349..12049)
                     /locus_tag="ACG8H8_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985482.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="ACG8H8_000381"
     CDS             12556..13716
                     /locus_tag="ACG8H8_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8985483.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="ACG8H8_000382"
     CDS             13732..14175
                     /locus_tag="ACG8H8_000382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985484.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="ACG8H8_000383"
     CDS             complement(14658..15188)
                     /locus_tag="ACG8H8_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP8985485.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="ACG8H8_000384"
     CDS             15293..15844
                     /locus_tag="ACG8H8_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8985486.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="ACG8H8_000385"
     CDS             complement(15997..16470)
                     /locus_tag="ACG8H8_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8985487.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="ACG8H8_000386"
     CDS             16608..18080
                     /locus_tag="ACG8H8_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP8985488.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="ACG8H8_000387"
     CDS             18077..18787
                     /locus_tag="ACG8H8_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP8985489.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="ACG8H8_000388"
     CDS             19139..19423
                     /locus_tag="ACG8H8_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP8985490.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="ACG8H8_000389"
     CDS             complement(19740..20123)
                     /locus_tag="ACG8H8_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP8985491.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="ACG8H8_000390"
     CDS             complement(20125..20709)
                     /locus_tag="ACG8H8_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP8985492.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="ACG8H8_000391"
     CDS             complement(20756..22231)
                     /locus_tag="ACG8H8_000391"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP8985493.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="ACG8H8_000392"
     CDS             22412..22723
                     /locus_tag="ACG8H8_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985494.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="ACG8H8_000393"
     CDS             complement(22838..25174)
                     /locus_tag="ACG8H8_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP8985495.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="ACG8H8_000394"
     CDS             25316..25984
                     /locus_tag="ACG8H8_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8985496.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="ACG8H8_000395"
     CDS             25987..27030
                     /locus_tag="ACG8H8_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8985497.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="ACG8H8_000396"
     CDS             complement(27267..28148)
                     /locus_tag="ACG8H8_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8985498.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="ACG8H8_000397"
     CDS             28307..29035
                     /locus_tag="ACG8H8_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP8985499.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="ACG8H8_000398"
     CDS             29191..29508
                     /locus_tag="ACG8H8_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8985500.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="ACG8H8_000399"
     CDS             29501..30109
                     /locus_tag="ACG8H8_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP8985501.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="ACG8H8_000400"
     CDS             30164..30955
                     /locus_tag="ACG8H8_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP8985502.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="ACG8H8_000401"
     CDS             31093..31581
                     /locus_tag="ACG8H8_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP8985503.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="ACG8H8_000402"
     CDS             31632..32504
                     /locus_tag="ACG8H8_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP8985504.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="ACG8H8_000403"
     CDS             32680..33042
                     /locus_tag="ACG8H8_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8985505.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="ACG8H8_000404"
     CDS             33051..33803
                     /locus_tag="ACG8H8_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985506.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="ACG8H8_000405"
     CDS             33817..35619
                     /locus_tag="ACG8H8_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985507.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="ACG8H8_000406"
     CDS             35896..36936
                     /locus_tag="ACG8H8_000406"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8985508.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11456 a         6547 c         7633 g        11312 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaa
//
LOCUS       DAZKAO010000010        36569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000010 DAZKAO010000000
VERSION     DAZKAO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.010"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8H8_000407"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8H8_000407"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP8985509.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8H8_000408"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8H8_000408"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP8985510.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8H8_000409"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8H8_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP8985511.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8H8_000410"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8H8_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP8985512.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8H8_000411"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8H8_000411"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP8985513.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8H8_000412"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8H8_000412"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP8985514.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8H8_000413"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8H8_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP8985515.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8H8_000414"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8H8_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP8985516.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8H8_000415"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8H8_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP8985517.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8H8_000416"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8H8_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8985518.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8H8_000417"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8H8_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP8985519.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8H8_000418"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8H8_000418"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP8985520.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8H8_000419"
     CDS             15402..16376
                     /locus_tag="ACG8H8_000419"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP8985521.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8H8_000420"
     CDS             16546..17946
                     /locus_tag="ACG8H8_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP8985522.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8H8_000421"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8H8_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985523.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8H8_000422"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8H8_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8985524.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8H8_000423"
     CDS             19454..21253
                     /locus_tag="ACG8H8_000423"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP8985525.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8H8_000424"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8H8_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP8985526.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8H8_000425"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8H8_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP8985527.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8H8_000426"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8H8_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP8985528.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8H8_000427"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8H8_000427"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP8985529.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8H8_000428"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8H8_000428"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP8985530.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8H8_000429"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8H8_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP8985531.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8H8_000430"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8H8_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP8985532.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8H8_000431"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8H8_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP8985533.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8H8_000432"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8H8_000432"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP8985534.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8H8_000433"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8H8_000433"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP8985535.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8H8_000434"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8H8_000434"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP8985536.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8H8_000435"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8H8_000435"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP8985537.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8H8_000436"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8H8_000436"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8985538.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8H8_000437"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8H8_000437"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP8985539.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11990 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatt aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZKAO010000011        35745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000011 DAZKAO010000000
VERSION     DAZKAO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.011"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..873)
                     /locus_tag="ACG8H8_000438"
     CDS             complement(4..873)
                     /locus_tag="ACG8H8_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP8985540.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            1026..2486
                     /gene="rhaB"
                     /locus_tag="ACG8H8_000439"
     CDS             1026..2486
                     /gene="rhaB"
                     /locus_tag="ACG8H8_000439"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP8985541.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            2483..3739
                     /gene="rhaA"
                     /locus_tag="ACG8H8_000440"
     CDS             2483..3739
                     /gene="rhaA"
                     /locus_tag="ACG8H8_000440"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP8985542.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            3753..4598
                     /gene="rhaD"
                     /locus_tag="ACG8H8_000441"
     CDS             3753..4598
                     /gene="rhaD"
                     /locus_tag="ACG8H8_000441"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP8985543.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            4600..4911
                     /gene="rhaM"
                     /locus_tag="ACG8H8_000442"
     CDS             4600..4911
                     /gene="rhaM"
                     /locus_tag="ACG8H8_000442"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP8985544.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            4993..6144
                     /gene="fucO"
                     /locus_tag="ACG8H8_000443"
     CDS             4993..6144
                     /gene="fucO"
                     /locus_tag="ACG8H8_000443"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP8985545.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            6271..7029
                     /locus_tag="ACG8H8_000444"
     CDS             6271..7029
                     /locus_tag="ACG8H8_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP8985546.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            7129..7773
                     /locus_tag="ACG8H8_000445"
     CDS             7129..7773
                     /locus_tag="ACG8H8_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP8985547.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            7780..8787
                     /locus_tag="ACG8H8_000446"
     CDS             7780..8787
                     /locus_tag="ACG8H8_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP8985548.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            8803..9645
                     /gene="kduI"
                     /locus_tag="ACG8H8_000447"
     CDS             8803..9645
                     /gene="kduI"
                     /locus_tag="ACG8H8_000447"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP8985549.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            9665..10468
                     /locus_tag="ACG8H8_000448"
     CDS             9665..10468
                     /locus_tag="ACG8H8_000448"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP8985550.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(10717..11781)
                     /gene="fni"
                     /locus_tag="ACG8H8_000449"
     CDS             complement(10717..11781)
                     /gene="fni"
                     /locus_tag="ACG8H8_000449"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP8985551.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(11778..12863)
                     /locus_tag="ACG8H8_000450"
     CDS             complement(11778..12863)
                     /locus_tag="ACG8H8_000450"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP8985552.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(12876..13853)
                     /gene="mvaD"
                     /locus_tag="ACG8H8_000451"
     CDS             complement(12876..13853)
                     /gene="mvaD"
                     /locus_tag="ACG8H8_000451"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP8985553.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(13846..14790)
                     /gene="mvk"
                     /locus_tag="ACG8H8_000452"
     CDS             complement(13846..14790)
                     /gene="mvk"
                     /locus_tag="ACG8H8_000452"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP8985554.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            15106..15765
                     /locus_tag="ACG8H8_000453"
     CDS             15106..15765
                     /locus_tag="ACG8H8_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8985555.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            15850..16755
                     /locus_tag="ACG8H8_000454"
     CDS             15850..16755
                     /locus_tag="ACG8H8_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8985556.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(16757..17389)
                     /locus_tag="ACG8H8_000455"
     CDS             complement(16757..17389)
                     /locus_tag="ACG8H8_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP8985557.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    17540..17665
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17709..18233
                     /gene="infC"
                     /locus_tag="ACG8H8_000456"
     CDS             17709..18233
                     /gene="infC"
                     /locus_tag="ACG8H8_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP8985558.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            18305..18505
                     /gene="rpmI"
                     /locus_tag="ACG8H8_000457"
     CDS             18305..18505
                     /gene="rpmI"
                     /locus_tag="ACG8H8_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP8985559.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            18558..18917
                     /gene="rplT"
                     /locus_tag="ACG8H8_000458"
     CDS             18558..18917
                     /gene="rplT"
                     /locus_tag="ACG8H8_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP8985560.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            19169..20506
                     /locus_tag="ACG8H8_000459"
     CDS             19169..20506
                     /locus_tag="ACG8H8_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP8985561.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            20554..22683
                     /locus_tag="ACG8H8_000460"
     CDS             20554..22683
                     /locus_tag="ACG8H8_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP8985562.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            22658..23347
                     /locus_tag="ACG8H8_000461"
     CDS             22658..23347
                     /locus_tag="ACG8H8_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985563.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(23755..24368)
                     /locus_tag="ACG8H8_000462"
                     /pseudo
     CDS             complement(23755..24368)
                     /locus_tag="ACG8H8_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(24504..24698)
                     /locus_tag="ACG8H8_000463"
     CDS             complement(24504..24698)
                     /locus_tag="ACG8H8_000463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985564.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(24748..25188)
                     /locus_tag="ACG8H8_000464"
     CDS             complement(24748..25188)
                     /locus_tag="ACG8H8_000464"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP8985565.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(25192..26037)
                     /locus_tag="ACG8H8_000465"
     CDS             complement(25192..26037)
                     /locus_tag="ACG8H8_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP8985566.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            26186..27604
                     /gene="pepV"
                     /locus_tag="ACG8H8_000466"
     CDS             26186..27604
                     /gene="pepV"
                     /locus_tag="ACG8H8_000466"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP8985567.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(27661..28608)
                     /locus_tag="ACG8H8_000467"
     CDS             complement(27661..28608)
                     /locus_tag="ACG8H8_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8985568.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(28831..29181)
                     /locus_tag="ACG8H8_000468"
     CDS             complement(28831..29181)
                     /locus_tag="ACG8H8_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP8985569.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            29381..30460
                     /gene="pepA"
                     /locus_tag="ACG8H8_000469"
     CDS             29381..30460
                     /gene="pepA"
                     /locus_tag="ACG8H8_000469"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP8985570.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            30604..30924
                     /locus_tag="ACG8H8_000470"
     CDS             30604..30924
                     /locus_tag="ACG8H8_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP8985571.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            30946..31410
                     /locus_tag="ACG8H8_000471"
     CDS             30946..31410
                     /locus_tag="ACG8H8_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8985572.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            31615..32220
                     /gene="ytpR"
                     /locus_tag="ACG8H8_000472"
     CDS             31615..32220
                     /gene="ytpR"
                     /locus_tag="ACG8H8_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP8985573.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(32259..33548)
                     /locus_tag="ACG8H8_000473"
     CDS             complement(32259..33548)
                     /locus_tag="ACG8H8_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP8985574.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            33976..34455
                     /gene="rlmH"
                     /locus_tag="ACG8H8_000474"
     CDS             33976..34455
                     /gene="rlmH"
                     /locus_tag="ACG8H8_000474"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP8985575.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            34540..35049
                     /locus_tag="ACG8H8_000475"
     CDS             34540..35049
                     /locus_tag="ACG8H8_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP8985576.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            35149..>35745
                     /locus_tag="ACG8H8_000476"
     CDS             35149..>35745
                     /locus_tag="ACG8H8_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985577.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGD"
BASE COUNT        11338 a         6539 c         7605 g        10263 t
ORIGIN      
        1 gaatcagctg aatttacgaa agtctctcgg cgatagtcct gtttttgctt tgaacaggcg
       61 gctaaagtaa gcaaaattct caatccctat ttcttcggaa atcattcgaa ccgtaaattc
      121 tgtataccga agaaggtctt tagcgtaaga aagttttttg ctgatcaaat aatcgattgg
      181 tggagaaccg ataaatttgg aaaaatcttt gctcagctga tatttattca ataagaattg
      241 ttgttctaat tgatcaagtg taatcgtttc tttgaaatgt tcgtctaagt attgcttcag
      301 tttgtaaacg taggcgggaa tatcctcttc ttcgaaatct agctgatact tttgcaaaat
      361 cagttcattc agcaactcgt ggatagctac agaggactgg aaatcggtac gggcattcgt
      421 gccttgttgc atgtggatca attctgtgat caaatggtgg atcttgtttt ctgatggatc
      481 ccctgttgaa tggaaggtgt tatggccgtc ttttgtgaac tcctgataaa atgattggac
      541 gttctgacca ttcaaatgga tccaatccat ttcccatggt tcttcgctga tcgtttgata
      601 acgctgatat tcttggcagt caataaaaaa taagtctcca gcttttagct gttgctgatt
      661 atcattatat gtaagcgaac ctttgccaga caaagtaaac tttatcaagt aagaaggtaa
      721 atgtgctcgt tcagtatagt aagggattgg tgctttgaag taaccgattt cttggatata
      781 aaaaaataaa ttcctgcttc tttgtgtagg cgtcaagatc actctttttg agtcttccga
      841 ccatgagtgg ttggcttttt cccagtaatc catgtaaaca ctcccaactt gtcaagataa
      901 tgcaattata taccaaaata gtgtgaagca aaatataaga ttctgtatta tgatagcatt
      961 gttaataaat gataacgctt tattaataaa gtgctaatca acaataaagt aaaagaggtg
     1021 gagaaatgaa tccaacattt gttgccatcg atatcggtgc atcaagcgga cgactaatga
     1081 aaagtacgag ggatgcaaaa ggttctttga tcttggaaga agtacaccgg ttcaaaaacg
     1141 gctttcggaa caaagacggt cacgatcgct ggaagatcga ttatcttatc aaagagattt
     1201 tgattggatt tgaaaaatta aagcagacag ggattgatcg cctttttgcg gggatcgata
     1261 catgggcagt cgattactgt ctgattgatg aacaagggga tcggataaat gatccgattg
     1321 cttatcggga caagcgtacc aatggagcag tagaacagtt ttctgaaatc tattctctag
     1381 ataagttgta tgagcagaca gggattcaga tccagccttt caataccttg ttccaattgt
     1441 ttgctgaaga aaaagaaaat ttggcaaaag cacataaatt gttactgata cctgattatt
     1501 tagggtatgt ctttactggt aacatggtga cggagaaaac taatgcatct actatgcagc
     1561 tgctgaatgc agggacaaaa gaatgggaaa tagaacttct tgaaaaaatc ggtgtctcga
     1621 aggaattatt tccaccattg acagagccgg gaacgatact aggagatctg caaaaagaac
     1681 aattttcaaa atatgatttg ccagatgtga cctttatcac agtagcaagt catgatacgg
     1741 cgtcagctgt attaggaacg ccaggtcaag gagaacgttg gggttatatc agcagtggga
     1801 catggtcgct tttagggatt gaaacaaaga tcgccaatgt ttctcgcaat gctttttctg
     1861 aaaattatac gaatgaatgg ggtgcccata acacggtgcg ttttttgaaa aacatcatgg
     1921 ggatgtggtt gatccaggaa gttgcccgaa tgcaagagta cgagtacagt tatgcggaat
     1981 tggcagagct tgctgcaaaa gaaaaaccgc tgcagcaagt gatggaaatc aatgaccctc
     2041 gatttttgaa tcccccaaat atgatagaag agattcagca atactgtttt gaaacagagc
     2101 agccagtccc tgtctcatcg ggagctatcg ctcgttgtgt gtatgataat cttgctcttt
     2161 gttacgcaca agagctgatc aaagtagaac acttaactgg aacgaaattg gaaaagctgc
     2221 acatcgtagg gggcggatcg aataatcgtc tactgaatca attgacagca gattatgccg
     2281 gaatcattgt agaagcagga cctagtgaag ccacagcaat cgggaatgtt gccatgcaaa
     2341 tgctcagtac aggtcagttt tcgaatatag aacaagcaag aaatgtaatc aaacagtcgt
     2401 ttccatgtga aactttttac ccagatgaaa ataagcaaaa cacgatggaa tggtacgtga
     2461 ataaaatcaa aggagtaatc gtatgaacac gattgaaaga aattacgaac aagcaaaaga
     2521 aaaatatgca actatcggtg tagatacaga tgctgtacta gaaaagatac aggacatcaa
     2581 aatttctatg cattgctggc aaggagatga tgtcaaaggt ttcttgacac cggatggcga
     2641 attgaccggt ggaatcatgg cgacagggaa ttttccagga gccgcacgaa cacctgaaga
     2701 gcttagacaa gatctggaga aggcttattc gttgataccg ggcaaacaca aattgaacct
     2761 tcatgctatc tatttggata cagaagaatc tgtcgatcta aatgaaattg agccgaaaca
     2821 ttttgaaaaa tgggtagagt gggcaaaaaa agaagaaatc ggtcttgact tcaatccgac
     2881 tttcttttcg catccaatga tgaaagacgg ctttacattg gctcatccag ataaagaagt
     2941 tcgtgaatac tggatcgaac acgggaaaag aagccgcaaa atcgcggagt actttgggaa
     3001 agaaactggg caaacaagta tcaataactt ttgggtacct gatgggatga aagataatcc
     3061 gattgatcgt tttaccccac gggaacgttt aatggaatca ctcgatgaaa tttttacaga
     3121 agagttagat gaacggtatg tacaagatgc agtagaaagc aaactatttg gtttaggcgc
     3181 tgaagcttat acagtcggtt cccatgaatt ttatatgggt tacgggttga ctcgtgacaa
     3241 acttgtttgt ctagatgctg gacattttca tccaacagaa gtgatttcca ataaattatc
     3301 ttcattagct ttattcagca aaggaatcat gttgcatgtt tcccgtccag tgcgttggga
     3361 ttcagaccat gtggttttga tggatgatga attgcaggaa atcgctaaag aattagtccg
     3421 taacgaattg ttggaaaaaa caaatatcgg tctggatttc tttgatgcaa cgatcaaccg
     3481 tatcgctgct tgggtcatcg gtacgagaaa tacacaaaaa gcactgctga aagctatgct
     3541 ggaaccagtt gagcgattga aagaaatgga gctggctttt gactttactt caagaatggc
     3601 ttatacggaa gaattgaaag atttcccgta tgccgatgta tggaattatt tctgttacaa
     3661 aaatcaagtt ccagttggtt tggattggct ggaagaagtc caggaatatc aaaaagacgt
     3721 gttggaatta agaaaatagg agtgtggcta tattgaaaaa aatagatatt ttacaagcgc
     3781 cttatgtgaa agaaatgatc gaaacgacgt cgaatcttta tcgcttggga tgggatgaaa
     3841 gaaatggcgg gaatatttct tatttactaa aagaagaaga gatcacaccg ttcttggatc
     3901 ctgaaaaagt gatccgccgc atctcgatga tttttgatgc cagccgttta gcaggatgct
     3961 attttattgt aactggttca ggaagatatt ttaaaaatgt agcgagcgaa cctgccgaga
     4021 atcttggaat cgttcgagtt gctgaagatg gcaaaacact ggaacttttg tgggggctgg
     4081 aaaacgaatg ccttcctaca agcgagttgc caagtcattt catgagtcat atcgcccgtt
     4141 tagaagtaga cccagataat cggatcgtta tgcattgcca tgctagtcat ctattagcga
     4201 tgagttttac gcatgaatta gacgaacgtt cctttagtcg tacgctatgg caaatgtgta
     4261 cggaatgctt agttgttttt ccagaaggaa tttccatcat tccgtggatg gttccgggaa
     4321 caaatgagat tggggaagca acagctgaaa aaatgaaaga aactcgattg gttttatggc
     4381 cacaacatgg aatctacggg tcaggaaaag acatggatga agtctttggt ttgatcgaga
     4441 cagcagaaaa ggcagcagaa gtctatacgt acgtcaaagc gcaaggacca atcttgcaaa
     4501 caatcacgga cgaaaacctg tggcatttag cagatgcgtt tggtgtcact ccaaaagcag
     4561 gttatttgga ggaagttcat acgaaagcag gtgtctgaca tggtgaaaaa agctttttgc
     4621 atgaaagttt acccagaaaa acaagaagaa tacaaaaaaa gacatgatga actttggcca
     4681 gaaatgcgta cagcacttcg tgaacacggg gtcatcgact attcgatcta tcttgatccg
     4741 agtacaagtt tgttatttgg ttacttagtg atcgaagatg aaaagttatg ggaaaaaatg
     4801 gcaaatacat ctatcaatca aaaatggtgg aagtacatgg aagatatcat ggagacaaat
     4861 ccagatgcct ctccagtcag ttgggaattg atgccagtct ttgaactgta gcaacaagca
     4921 actgggcaac cggttgcttg gggttctttc gtttttattg tgaaaaaaaa tacaaattag
     4981 gaggaataga aaatgagtaa tcgaatgatt ttaaatgaaa catcgtattt tggaaaagga
     5041 gcgattgagt cgatcgtccc tgaatttcag cagcgaggat tcggcaaagc agctgtcatt
     5101 actgacaaag gattgatcga gcacggtata gcaacaaaag taacagaact tctagataaa
     5161 gcagcaatcg cttatgcttt atacgacggg atcgtaccta atccaacgat ccaaaatgta
     5221 aaagatggag tggcttttgt taaagaggca gaggcagact gtttgatcgc aatcggtgga
     5281 ggttcgccaa tcgatacggc aaaagccatt gggattattt tgacgaatcc agagttttca
     5341 gacgtcatta gtttagaagg tgttgctgat acgaaaaatc catgtttacc tatattagct
     5401 ataccaacga cttctggaac agcagcagaa gtaacgatca actacgtcat cacagatgaa
     5461 gtaaatcacc gaaaatttgt ttgtgttgat cctcatgata tcccaatcgt cgcgttcatc
     5521 gacagcgata tgatgatggg aatgccgaaa aaattggctg catcaacagg tatggatgct
     5581 atgacgcatg ctattgaagg gtttatcaca aaaggcgctt gggaaatgac tgatatgctg
     5641 catttaaaag caatcgaaat catcggccat tctttggaag tttctgtaga tggcgatcaa
     5701 aatgggcgag aaaaaatggc attaggacag tatattgcgg gaatgggctt ctcaaatgtc
     5761 ggtttaggac tggtacacgg gatggctcac ccgttaagtg catggtacaa cattcctcat
     5821 ggcgtagcgt gtgcagcact tttgccaact gtcatgaaat ataacaaaga atatactgga
     5881 gaaaagtacc gtgagatcgc tcttgttcta gggattaaag gagcagctga aatgtcttta
     5941 gaagatgtaa gagaagcggc ttgtggtgag atcgatcggt tgagcaaagc ggttggaatc
     6001 ccagaaacca tttctgaatt aggtgtaaaa gaagcagata ttccagcaat tgcagaagat
     6061 gctttgcgag atgtatgcac accaggaaat ccaagagaaa cgacagtaga agaaatcatt
     6121 gctttatatc aaagtttgat gtaaaaacga agcggatctt caacattaaa ttgcctgtaa
     6181 agagaaatgg tagtttatat ctggatatct cttttaaatt gatggtattt ccgctataat
     6241 ttttcttata aagaaatagg agaatattat atggaaaata aaaagcctta tggaactgta
     6301 ttgatcaaag cctctaaaat actagactat ttagcggaaa accaagatgt ctctttgcag
     6361 acgatcgcaa agggtgtggg aatgacctct tctacagtct tgaaaatact agatactttg
     6421 ttgatgatcg gctacgtaaa taaaaattca gagaaaaact atcgtttagg tgcaaagctt
     6481 attcgctacg cgaataaaaa cattgaacaa atcgatttgg tagagttgac tttaccgttt
     6541 ttagaacagc tgcagcaaaa gatcgatgag actatccact taggggtatt ggacaacaat
     6601 gagatcttgt acgtgaacaa actagatcca aaacatcaaa cgatccgcat gtcttcaaaa
     6661 atcggaatca cccgaccatt atacaattct gcgatgggca aagccgtatt agcagaattc
     6721 agtgaagagc agtacactga ttatcttgaa aagcatccgt tgatccctta cacagaatat
     6781 acgatcacca atgcattacg gttgaaaaag gaaatccaaa cggtaaaaga aacccacatc
     6841 gcatttgacg atgaagaaat cgaacgtgat attttttgta taggagcctc aatcgtgaaa
     6901 aacgaacaaa tcattggtgc atttagtgta agtatgccga aatatcgttt aacagatgag
     6961 acaaaagaac aaataatcag tgccctcaag caaacaaaaa gcgaaatcga gcaaagaatt
     7021 caaaaataat gaaaaaagcg tttgcaattt ttcttttctg ccgtataata tgggtataga
     7081 tttccaagtt ataaaataaa atttctaaat aggaaatagg tggagaagat gaaaaaaatt
     7141 gatattttat ctcgattgaa aaacgccgga gtgattgcgg tggtacgcgg aaaaagcaaa
     7201 gaagaagctt tgaatgcttg ccacgcaatc atcaaaggtg gtctaactgg tattgagttg
     7261 acttttacag tccctcaagc agatcaagta atcaaagaac ttttgtcatt ctacaaagac
     7321 caaccagaaa tagtgattgg cgcaggcaca gtattagacg ctgtcacagc tcgcttggct
     7381 atcttagctg gcgcagagta tattgttagc ccttcctttg atcaagaaac ggcagagatg
     7441 tgcaatctgt atcaaattcc ttacttacca ggatgtatga ctattaccga gatcaaaaca
     7501 gcgctgaaaa gcggggtaga tatcgtcaaa ctatttcctg gaagcgctta tggaccaagt
     7561 atcatttctg ccttcaaggc gccgatgcct caagtcaata tcatgccaac tggcggtgtg
     7621 agtctggaca atatgaaaga gtggttcgat gctggtgtcg taacagtcgg agtaggtggg
     7681 aaccttttag cacccgcagc tacaggagat tttgacaaag taacggaagt cgcgcaacag
     7741 tatgcagcaa aaatgaaaga aatcaagagg tagctgctta tgggaaaagt agtgacactc
     7801 ggagaaatca tgttgcgatt gtccacagag cagggaaccc gtatcgattc aagtgaacga
     7861 ttcgctgccc attatggcgg aggggaagca aacgtagcaa tttctttagc taattacgga
     7921 catgaagcga tttttgctac aaaagtacct gataacgcat tgggaaaagc agtcaaaaag
     7981 cacttgcaat gttatggagt agatacgaaa caggtattat ctggtggatc aagactaggt
     8041 acttattata tggaaactgg tagtggcgaa cgcgccgcct cagtgatcta tgatcgtgcc
     8101 ggatcaagtt ttgctgaaat ggatcatttg gaatggtcgc tagaggagtt attccaagga
     8161 atagatatat ttcatgtttc aggaatcacc ccagctttgt ctaaaaaatg gcaaaaaatg
     8221 acgaaacagg tgatagaagc tgcaaaaaaa gctggctgcc tgatcagctt cgatatcaac
     8281 tatcgtggaa aattatggac acagcaagaa gccagcgaag taatccatca gcttttacct
     8341 ttagtagata tctgctcagc tggaaaaatg gatgctcttt atttgttagg tatcgagaaa
     8401 gcgccagaag cagaagaaca acctctaatt tattattatc aaaaaatgca ggaaaagttc
     8461 ccgaatattc aggtgtttta ttcgaccaag cgtaccgttc attcagcaag tgaaaatgaa
     8521 ttgaagggga cattgtggat gaaccatgaa tatgtggaat cccaagtcca tagaatcacc
     8581 ccaatcgtgg atcgagtagg tggcggagat gcttttgcag gtggcgtgct ccatgggatt
     8641 ttatccaata tgtctccgca agaaatcatt gattttgcta cggcagcttc tgccttgaag
     8701 catacgatcc atggggactg caaccaattt agtcaagaag aagtcgcaca atttttagca
     8761 tgcggctctg gaaaaatcat tcgatagaag gagtagaaga aaatgcaaga aatggaaaca
     8821 cgttatacac acagtccaga agatatccgt cattattcaa cggaacaatt gagaaaagaa
     8881 tttttagtag aaaaggtatt tgaaccaggg aaaatcagtt tgacttatac gcacaacgat
     8941 cgaatgatct ttggtggagt gacacctact gaaaaagaat tagaaatcac attagacaaa
     9001 gaactaggtg ttgattactt cttagagcgt cgtgaactag gtgtgatcaa tatcggtgga
     9061 ccaggattca tcgagatcga tgggcaaaaa gaagaaatga aaaaacaaga cggatactat
     9121 atcggaaaag aaacacgaca tgttgtattt tcttctcaag atgctgaaaa cccagctaaa
     9181 ttctatatta gttctgttcc agctcatcat aaatatccaa atgtaaaaat cagtatcgac
     9241 caagtaaaac caatggagac tggtgaaggc ctgacactga atgaacgtaa aatctaccaa
     9301 tacatccatc caaatgtatg cgaaagctgt caattacaaa tgggttatac gattttagaa
     9361 ccaggaagcg cttggaatac gatgccttgt catacacatg aacgtcgtat ggaagcttat
     9421 gtctactttg attatgccaa tgaagataca cgtgtgttcc acatgatggg caaaccagat
     9481 gaaacaaaac atttagttgt agacaatgaa caagccatta tttcgccaag ctggtcgatc
     9541 cattcaggtg taggtacaag caattactca ttcatctggg caatgtgcgg agaaaatatc
     9601 acctatacag acatggatat ggtgcaaatg gatcaattaa aataagcata agggagagaa
     9661 aatgatggaa tttagtatgg atatgtttcg tttagatgga aaagtagcat tagtcaccgg
     9721 tgctgtctat ggtattggat ttgagattgc acgctcttta gctacagcag gagcaaaaat
     9781 cgtatttaat aacttgaatc aagaatcagt tgatgaaggt atagcgcatt ataaagaagc
     9841 aggcatcgat gcaaaaggtt atgtctgcga tgtgaccgat gaagaagcgg ttcaagcgat
     9901 ggttcaacag atcaaagaag atgtaggttc agttgatatt ctagtcaaca atgcagggat
     9961 catcaaacgg acaccgatga tcgaaatgga tgcggcagac ttccgccaag taatcgacgt
    10021 ggatttaaat gccccattca tcgtttcaaa agcagttatt ccggatatga tcgaaaaagg
    10081 cggcggtaaa atcatcaata tctgctccat gatgagtgaa ttaggacgcg aaacggtcag
    10141 tgcttatgct gcggcaaaag gcggattaaa aatgctgacg aaaaacatcg cttctgaata
    10201 cggtcaatac aatatccaat gtaatggaat tggaccaggt tatattgcta caccgcaaac
    10261 tgctccatta cgcgaaacac aagaaaacgg tgaacgtcat ccattcgatc aatttatcgt
    10321 tggacgtacg ccagcagcac gctggggcga gccaatcgat ttggctggac catctgtctt
    10381 cttggcttct agcgcatctg atttcgttaa cggccatgtc ttgtatgtgg acggaggcat
    10441 acttgcctat atcggaaaac aaccttaaaa gagctttagt acactgttta gtggagaggt
    10501 tgtgaaatca gcggattgaa cgaataccga aggacagctg attaaacatc atttattcga
    10561 aaaaagaggc tgggacagaa gcatttaact ccaagaaata agaaggaatt cacgaaaatt
    10621 gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgctttt acttccgccg
    10681 tttgtacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttc
    10741 tgtgcgtctt ccatacttca ctaagtcgat ccctcttgct tcgcaccaat ccttcactgg
    10801 tccggaaaag atcagagatt gttggtgtag ggccgcggta ttcgttgcgc cgaccatagt
    10861 ataaagcaga cgaagctctt cttgccattg cttcatcagt ataatagttt cttctacgcc
    10921 gtgattcatc agatgcgtca gtacggttcc tgaagtaccc acggcatttg cgccgagaca
    10981 aagggcttta aatatgtcat aggcattgcg gatacctccg gaggctagga tttccataga
    11041 agtgtctgct tcatttgctt caagcaatga agagactgtc gattgccccc agtcagctaa
    11101 ataactcagt tcccgcttgg agcgacgggc gttttcgatc tgggtgaagc ttgttccgct
    11161 tcgaccgctg atatcaactg tatgaactcc taaagcagct agatcattga gcgtttctct
    11221 tgtcatgcca aaaccgactt cttttacgat gagggggacg tcaatggctg tttgagtttc
    11281 ttgaataagt actttccaat tggtaaaatc acggtctccc tcaggcataa ccaactcttg
    11341 aggtgcattc aaatgaatct gtaaggcatc tgcgtgaaac aatcggatag cttctttggc
    11401 tctttcgaca gaagtccctg caccgatgtt ggcaatgatt ttcccgtgag gatattcttg
    11461 tcgcataatc gtataagtat cggctaaaga agggactttc aaggcagcgc tgactgaacc
    11521 agtggcaatc atcaaatctg cttctcttgc gacgattgca agatcttggt tgattttttt
    11581 tgttttttcg cttccgccag tcatcgcatt gatataaaat gggctgctta gtgtaaaccc
    11641 aactgcctgt gtacttagat cgacatcaga aactgctatt tgcggcaaag ggttatggat
    11701 gatccgtacg aagtcaaatt cgtttttttg tttatcatga aaggctttag ctagtgaaac
    11761 atgttcgtct tttcgattca tcggttttcc tttctttgat cgctatagac atgtaacggc
    11821 agtggagtga tttctgcttt ttcccatgca ctcattaaag gaagaatgcc agatttctgg
    11881 tcaacaatta cgattccgca atcgccccca cctgcaccag aagattttgc ggctccttca
    11941 tactgttcag ctaaattaca caatttgttc aaagcaggcg tttcgatgac gaccccagtg
    12001 attgcagata aatcatgcag taattgtcgg ttttttcgaa tcatctgttg gatcaacgtt
    12061 acattatttt ctttaaaccc tttgatcatt tcgttgacac attctgtact attttttaag
    12121 aaaagctgat aagccaccat tttatcttct ctcgaacggt gaacttgatc gaccaaatca
    12181 gaagtagagg cagggctacc cgtccaacca atcaataaac gtaaatcttc aggagcaatc
    12241 aatggctcaa tggatagacc tggccaatcc agggcaagta actcactgat agaatgctga
    12301 gtttcttgtt cttggagcca aggatggtcg aaggttgaga aagcgatcca gccaccataa
    12361 cagctagcag cgatgtcgcc gcaggaacca ttatcttgaa ctgctaaatt ggctagtgct
    12421 gcaatcttga aaatctccaa ctgagataaa ttcaacgcgt aaaaaacatt caaggctttg
    12481 accgttgcaa cggtcacggc accgctagaa cccaatccat atttccggcc atttgaactg
    12541 tctaactcac ttgttacttt cagatcataa aaggataaaa gaatgttttt ttcttgtgca
    12601 tacttttcag tcaagcgaat agcagcaagg atataatgaa aaggattttc ccgaatatct
    12661 aaaactaatt ctccgttgcg tcttgtccaa cgtacaggca tcccactata ttgtgcagat
    12721 tggatacttc cgacttttcg tgcagattct acagtgactg tcacgaattg atcgactgca
    12781 gcgataactg ctggatggcc tgtttcaaca actgcatatt ctccggcaat atagagtttg
    12841 cctggtgcag atacttcaat cataaaaatc tccctttatt caataatcgc aattcctgga
    12901 ccagcatgtg cagtaattac ttgttctttg ctaaataatt tggtaaatgt ttcttgtacc
    12961 tcagataaat ggctgttttc cactaaaact ttgacattag ggccagcatc catcgtaaag
    13021 tagcatggaa tgccttgctt tctcaattgt cgcaccgcat ccatcgcttt taagctgtca
    13081 ggagaccagt aagtgaatgg aggctgggca gctaaagtcg ttccatgcat ttttagtccg
    13141 tttctttcca tcgtttctcc gagaagttgg aaatcttttg ttttgattgc ttgtttcaat
    13201 tgatatagat ccccttcaac ggaatctaac cagccttgat aaaaactgga tgtttcgact
    13261 gtccgacgca tcccatttct gctggacact tcttttttct ggtcgttgat caaaacgaaa
    13321 accattgcta aatcgtcttc gaaagaatcg gaagggactg gcttagcgta agaacttaag
    13381 tcgtcatggc ctttttccca ttcgacgaaa ccaccgaaaa tacttcggca agctgaacca
    13441 gacccgcgtc gagcaaatct tgaaagagag agatcatcta atccaagctt aagagcagta
    13501 ttgcaagcgc ctgctaaagc agctagcccg ctggcagagg aggctaatcc agctgcggta
    13561 ggaacgaaat tctgactgat tactgaagct tttagtgaga ggccggcttc tttacgaaaa
    13621 aggtcaagaa actgactgac tttttttgtt gctttttcgt cttgcaaggt gccatctaga
    13681 taaaattcat ctaccatata gctatcagaa aagatgactt ctgtctctgt ataaaatgca
    13741 tccaatgtta acgaaaggct gttgttcatt ggaaggatga gttcttcatt tttcttaccc
    13801 caatatttga ttagagcaat gttggtatat gcgcgtgctt tgcctttaaa cataggtatg
    13861 tactcctaat ccttgtatcc aagtttcagc agcacccgca tcttcaagtg cttgactgat
    13921 ttcttgggct tttgtttttg tttgtgctaa tgcaatcata cagccaccgc gtccaccgcc
    13981 agttaatttg gcacccaatg ccccggtatc ttttgctgtt tggatcaata gattaagaaa
    14041 atcattgctg attgtcagct tttccagaag tgattgtgat tcatccattg tctgtgccaa
    14101 cgtttctggt gaattttcaa tgattgcttg ttttgcttgc ttcgtcaagt atcctaattt
    14161 ttggatcatt tgtccagttt catgaggctg ggtttcaaag aggtgagcga catctttgac
    14221 ggcttctctt gtttgtcctt tgattcctgt atcagcgaca atcaaaaaag catcgatgtt
    14281 caaagaaaag tagtcgaaag gatgcccttt tgtaaaataa atcggctgca agctgctagt
    14341 agcggctgca tcgattccac taggattacc gtgggcgatt ttttccgaaa gctggacatt
    14401 ttctaatagt atttcacgag acaaaggaaa tgctaagtaa tcataaaaag cacgagtgac
    14461 tgctgtggcg actgcagcgc ttgatcccat tccacgttca gcaggaatcg tgctttcaat
    14521 ggtcagttgc aagttttttt ggatgtcatg ctgttttttt aagtgaatga ataatttttt
    14581 gatatttttt aatgcatgag gagcttcggc taaatttcct gaatagtagg aagaaaccag
    14641 ttgatcgtcc attgcatcca gctcttcaag gaatgcggtg acttttgttg catagaaagg
    14701 aaaggcaatc gctggttctc cataaacaac cgcgtgctcg cccatcaata tgatctttcc
    14761 gcttgattct ccttggccat agtttgccat aatctaactc cttctcacgt aaaagtgatt
    14821 tttaagcaaa aaataaagcg cccaaaacga gttgcgctgc ttcacgaatt tttttgtcca
    14881 ctaagggtct caaaaaacat tcctccttta ttttactaaa accatctcag aagtaaaact
    14941 aagaatacgt tttctaaaga aaaaacactt ttttcttaag aattttttga ctttttttct
    15001 tctttgacta gaaaaaaata caattgacac tactagatat tgggggtata tttaaaaagg
    15061 ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag cgtgatcgtt
    15121 ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc tgaacacttt
    15181 gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt attttatgca
    15241 aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg ttttgcaagc
    15301 atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta tgaagattca
    15361 ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt tcgtgtgtat
    15421 gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa aaaacatccg
    15481 gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat cgaaaaacgt
    15541 ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta caaagaattg
    15601 aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat ccaaatcgat
    15661 ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt gaaaatgatc
    15721 gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac agttgctcaa
    15781 ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca aatgtcagga
    15841 ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg gatcaatgta
    15901 cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat tagtgacgag
    15961 atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga ttcttttgat
    16021 gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa tcattatgaa
    16081 actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac cacgccgaat
    16141 accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga catgagtgta
    16201 tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc tttaatcgag
    16261 gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac aacgaacaag
    16321 aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac aggaacaaaa
    16381 caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa gttgtcagaa
    16441 aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt tgaaatgacg
    16501 aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt gctgaataac
    16561 aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac cgttccaact
    16621 attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc cgttttcgga
    16681 tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat tgcactgtgg
    16741 cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg atcacaagtg
    16801 cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg attgctgtga
    16861 agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc gaaccggtca
    16921 actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg ctgtcttcta
    16981 attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa gccatcgaag
    17041 caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc gtacctgtta
    17101 atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc agattggaaa
    17161 aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact gttttttgca
    17221 tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg agatagcttt
    17281 ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa taaaactctc
    17341 cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg tgggttcacc
    17401 tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct gctaatctaa
    17461 tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga ttagtttttg
    17521 ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct tagttgataa
    17581 tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc gggttctgta
    17641 agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct cataatattt
    17701 ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt gcacgagagt
    17761 tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca gaagcattga
    17821 atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg aaaccaccag
    17881 ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa gaccgcgaag
    17941 cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct acgatcgact
    18001 tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag ggcgataaag
    18061 tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt ggtcaaaaag
    18121 tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa aaagcgaaaa
    18181 tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag taatttgaat
    18241 gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag gaggaaatat
    18301 agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac gtactggtaa
    18361 aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca aaacaaaaaa
    18421 acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca aacgtattcg
    18481 ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag atttattttt
    18541 attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt aaacgtcgta
    18601 aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta tttaaaacag
    18661 caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt caaaagaaac
    18721 gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg aatggcttga
    18781 gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac cgtaaaatgt
    18841 tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac caagcaaaag
    18901 aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg aaaagttatg
    18961 ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa taacgtataa
    19021 gtctaaaata gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt
    19081 aatgaaagcg ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc
    19141 agaaaaacaa aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt
    19201 tatctcattc tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt
    19261 tgccatcatg gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa
    19321 cccggcgatg gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc
    19381 agctgagtac gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt
    19441 tctagtagat agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga
    19501 caagccaaga atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat
    19561 gacaggaatc ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt
    19621 aggtatttca gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt
    19681 tgctaatatc gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc
    19741 tgaaagctat acttataatg actatttcca aattggtatg attgggatga ttgtaagctt
    19801 agttgtggta ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc
    19861 agcaaacgta ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt
    19921 accagtttta ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg
    19981 tatctgggca ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca
    20041 gtttgcgaaa ttatttacag atggcgcagt agatgttgca ccaatggttg gtttcttgat
    20101 gaccttagcg atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt
    20161 tggagattgg ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc
    20221 actaggattt ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt
    20281 cgtattagct gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac
    20341 aacgattgct cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta
    20401 tacaaaagta acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg
    20461 agcgatctta tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg
    20521 ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt
    20581 gatgttggcg gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc
    20641 ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt
    20701 cagtcttttc aaaattgttt gcgggaaaat aatatcagtc cagaagatgt tgtgtttgtt
    20761 gcacattcta ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc
    20821 gtaatcggga tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg
    20881 aacgatatcg atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc
    20941 aaacacttaa atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg
    21001 gaggtcttgg tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt
    21061 tatgaagcag catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta
    21121 tatggattga caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg
    21181 ctagatacgg caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg
    21241 atgattatgc gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt
    21301 ttaaccatgc tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg
    21361 tctaatggtg tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat
    21421 ggacgtccag caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta
    21481 gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc
    21541 atcgatgtcg gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag
    21601 acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct
    21661 gattacgtca aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca
    21721 gccaatgtcc ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca
    21781 cgaaaagcca tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa
    21841 cagatcatgg aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag
    21901 tatcacttgg agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg
    21961 attacttact ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg
    22021 atttcttcta ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct
    22081 tcgccaagta aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa
    22141 tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa
    22201 gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc
    22261 acaacagaag aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt
    22321 tgcttattgg aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat
    22381 gctggaagtc ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct
    22441 tcatgtatga aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat
    22501 atggcagttt atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca
    22561 agagtctctg attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa
    22621 gtatctacta tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca
    22681 taagttagaa gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta
    22741 ccagtttcgg cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat
    22801 acaacaatca atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt
    22861 tgccgatgta ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga
    22921 gcaaataaac gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat
    22981 gacggagccg ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc
    23041 gcaagctttg attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat
    23101 ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa
    23161 cattcgtgca ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga
    23221 tttttcgcct tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca
    23281 agggatctta gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta
    23341 caaataaaga gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacgatgt
    23401 tctcctgtca actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt
    23461 tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt
    23521 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt
    23581 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac
    23641 ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact
    23701 attttgccgt ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt
    23761 gatgggtctg tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg
    23821 ctcctcgata atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg
    23881 atcaatcatt gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc
    23941 tgaaatcgtc aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca
    24001 atcccaaatt tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa
    24061 atcaaaaaaa gcttttttat tttgagcgat tactgcctga tcggtttttc caacttttcc
    24121 cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata agtgccggac
    24181 tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc tcgaatcggt
    24241 gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact tccaggtgct
    24301 aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg caaattattg
    24361 ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa atcgcttaca
    24421 aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat gcctattaga
    24481 gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttgtatcgg tttgacacga
    24541 ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat gagcagattg
    24601 tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg cattacttta
    24661 acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac tcgacacaat
    24721 acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag aagcgtacgc
    24781 ttggaataac tgatctaact ccggattgac tttgctcagc ttcactaatc gttgcgaatc
    24841 agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt cttcagttcc
    24901 cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac ttttggatag ttggatggat
    24961 gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt ccagaacggg
    25021 gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta ccaataaggc
    25081 tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc ccatttgatc
    25141 tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga ctcatgcctc
    25201 gttttccatt gattttttac aaaaataatt catccatttc ggaagtgctt cgctgatttg
    25261 tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac tatgaagata
    25321 aatagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc ctgtgatcat
    25381 cccagctaaa gtgtcaccca tgccgcccgt tgccatagca gcagaaccta aaggattggt
    25441 gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga gcacagcacc
    25501 gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg gtagagaact
    25561 caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat agggtagcaa
    25621 aagatgatta gcagcaagta aacttaaagc agagccatca atcaccagcc attgttcttt
    25681 tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag tacccaatcc
    25741 gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt cagtatccgt
    25801 ccaaactaca atcattgctt ctggcagatg actgtgaaga gcactccgat ttttttcatc
    25861 agttactaca gtcgtgagac ccgctccgct gtgtacggca gctaaagcac tcataatgat
    25921 tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg ttcctttatg
    25981 tgaaattttt ggacgaggtg tgatgactct tttcactagt tgttcgttta tcagcatcta
    26041 aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag ataagaaaag
    26101 gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg aacacgaaga
    26161 cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag tagaagcacg
    26221 taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg agcgcgatga
    26281 ctcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat tgaaacatat
    26341 gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact atgcagggca
    26401 tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg atgtcgttcc
    26461 tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg gaaagatctt
    26521 tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg cgatgaagat
    26581 cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg gcagcgatga
    26641 agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac cagattttgg
    26701 tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg tttctttagc
    26761 tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg tttccggact
    26821 tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa gtgcagatgc
    26881 agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag taagcggaac
    26941 gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg cacatggtgc
    27001 aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag ataactatga
    27061 attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc atgaagattt
    27121 ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat tgacaatgaa
    27181 tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca tcaatatgaa
    27241 tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg aacaaacagt
    27301 ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac attacgtacc
    27361 aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca ctggagaaaa
    27421 aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac gtggcgttgc
    27481 ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg aatttatgag
    27541 tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc gtttggcgaa
    27601 ataattaatt aaaaagagga tcaagaattg caaattattt ttgcagtctt gatccttttt
    27661 ttactttcta ctgtggtatt catgataaac atgatataga ttcaattcat attctttctt
    27721 agcgttcgtg acgactgtat ccaaaatcaa ccctgttact aatgaaagaa gtgctaaagt
    27781 catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta ataaagcttg tcagcatata
    27841 ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa aagaagaaga tggtccaaat
    27901 cccaaagaaa agcaaaggct tataatcttt aaacatttta ataatcatca tgatcacttt
    27961 gaatccgtca gagaatgtat tcaattttga ttcgctgcct tcaggacgat cacgataatc
    28021 gattgggatt tcaacgaact tgaatttttt atctaaagca tggatcgtca attccgtttc
    28081 gatttgaaaa ccagaactca taataggaaa acttttgaca aagatacgat tgaatcctcg
    28141 atagccggtc atgacatcaa gaatttttgt tttatacatt ctagtaattg tatttctaac
    28201 aaggttattt ccaaaatcgt ggaaaggtct cttattctca tcaaagtagg ttccatttga
    28261 taggcgatca ccaatgacca tatctgcttc accagaacgt aatttctcga gcaaaccatg
    28321 tacagcttct gcaggataag tatcatcgcc atctaccatt aaataatagt cggcatcgat
    28381 atcaaagaac atttggcgga tcacatttcc ttttccttga cgaggttctt tttttacgat
    28441 tgctccacct tgaacggcca attcgtatgt tcgatcggtc gaattattat catagacata
    28501 aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg actgttgaaa ttgttgcttc
    28561 ctcattgtaa caaggaatca gaacagcaat tttttcttgt tgttccattt ttcactctcc
    28621 ttaactatag aaaacaaaag ctaaattaaa aatacctcat aatcaaagga aatgctagtt
    28681 tttcccgcta aatatcattt atttaaaaag aaaatactgg aatatatgtt tggaaagctc
    28741 tatctaaaaa accacccact acaaaagaag gacatgatac ctctcttttg aatagtgggt
    28801 gcgtattcgt tctctcactt ttttgacgta ttaatctgtt gcaccttttt ttcttgaaat
    28861 ttctaatacg gttcctgtat gagcatctgc taaaaattca taataaacag tttcgccatc
    28921 ttctattcga gaaagacctc cccgataagc actcgaccga acggcgaatt tcctaatagg
    28981 ttccttttca aaagagatcc aagaaccttc gattgcacct tcttttagaa aagatgactt
    29041 gatattttca agtacttggt ctgctgacaa tacttttttg cgatgatacc aaatcgttga
    29101 agctaccccg ctcaataatc ctagaccgat tccaactgct aacccgcctt taaaatagct
    29161 tagttcgctt tcttcataca tgtggacata cctccttctt ctcacttgtt actccttgtg
    29221 cttattttac cacactctca tattttcttt gaaataaata accgctacca gaaaaaataa
    29281 gctataataa atacgatcgg tctgcagaaa aaatcatatt ttatccgcag atcaatataa
    29341 gtctgagagg agtttagtct gaaatttaga cgaatagcat atggaagaaa aaacatttca
    29401 acgaatcaaa gagttaacag aattacaagg aacaagcggt tttgaacaag atgttcgcgc
    29461 ttatatggaa agtcatatgg aaccgctggt agatgagtta cagttagatg gtttgggcgg
    29521 catttttggt ctgcgccatc atgaagaagc ggatgcaccc cgtgtaatgg tcgcagcaca
    29581 tatggatgaa gtcggattca tgctgacaca aatccaagaa aacgggcttt ttaaagtcgt
    29641 acctctaggc ggctggaatc cttacgttgt ttctgctcag cgttttacac ttaaaacctc
    29701 tactggtaag aattatccat gtatttcttc ttccgttccc ccacatttat tgcgaggaac
    29761 aagcggacaa aaatcagtag aagtaaccga tattttgttc gatgctgggt ttgaatcaag
    29821 agaagaggca atgagttacg gtgtccttcc aggagacaca atcgttcctt acgctgaaac
    29881 gatcaaaaca gccaacggaa agaatattat cagtaaatca tgggacaacc gctatggctg
    29941 tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa acattaggtc atacattgat
    30001 tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt tctaaagctt ctgtcaataa
    30061 attcaagcca gatctgttct ttgcagtaga ctgctctgca gcagatgata cagtgacgaa
    30121 aaatggcaca ttcggacatt taggagaagg tacattgatg cgtatccaag atccaggttt
    30181 gatcatgctt cctcgtttgc gcgagtactt gctggatatc gctgaaacaa acaatatccc
    30241 ttatcaatac tttgtttcaa aaggcggtac agatgcaggt gctgctcata cacaaaacga
    30301 aggaatccca agtacagtga ttggcgtagt gggacgttat atccatacgc atcaaacgat
    30361 gttcagtatt cgcgattttg aagctgcacg agaaatgttg attcaaacac tgaaaggttt
    30421 ggataaatca acagtgaata cgatcgtata cggaaaataa tgcgatagtg aaactataaa
    30481 atctggcaaa agattgcttt ttgccagatt ttttttataa aaagaggatc aatgtttgtt
    30541 agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggacaa
    30601 agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaagagt
    30661 gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct
    30721 gaaattgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata
    30781 gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga
    30841 aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc
    30901 ctagaaagtg taacaaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat
    30961 aagaatattt tagtaggtat cgacggtagt gaacaagcac ttgctgcttt caaaaaagcg
    31021 gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa
    31081 ttttataact ttatgggcta tgcacctatt gatcaaaatc tagtcgatca gcaaactgaa
    31141 acagccaagg aaatgatcga agaatgtaaa gaatatggga aatctgttga ttacacaaat
    31201 atagaaggaa ttattgcata tggttctgtt cgtgaatcca tggcagtcaa attgccaaaa
    31261 aaatatgaga tcgatctaat catggttggg cagtcaggtc taaatgcagt cgaacgcttt
    31321 atgacaggaa gtgtagccag ctacgtgata cgtcattcac cttgtgatgt cttgatcgtt
    31381 tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtacgac ctttttaatc
    31441 aattaactaa aacagtaaag ggactgtgac aaaagtcttg tcacagtccc ttgttacgaa
    31501 taaatggtgt tccagaagag gcttcttcgg aaataaagca tgaaaaaaat ttcatgcagt
    31561 gaatgcaatt ttttgttaag ataaatctca gcaaaggata atggaggaaa gaacatgatt
    31621 tttgcttata acaaagcaca cgtcggagac accttgatgg ttattatagc cgacgataaa
    31681 ggaacagaaa acactgttga acgcaaatgg aatgttgctc ggatcaaaga tgaacaaggt
    31741 caagttgttg cttggaactt tttccatatc tccgatcatc taacaatcga agggaacggc
    31801 caagtaagct taacagatga ggaattagcg aaattgaatc gattgatcaa agaagcagga
    31861 ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg ttgtcgggta cgtaaaaaca
    31921 tgcgtcccac atcctgattc tgaccattta tcgattactg agacagaagt agacaatggt
    31981 acagtattgc aaatcgtttg cggtgcaccc aatatcgaag ctggacaaaa agttgtcgtt
    32041 gcaaaaccag gagctatgat gccagatgga atgatgatct ggccaggaac tttgcgaggg
    32101 gtagatagct atggaatgat ctgttccgct cgtgaattgc atttgccaaa tgctccagca
    32161 aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg gagaagcatt tccaaaataa
    32221 agaggttgtg acaagtcttt gtcacaacct cttttttatt aattttcact attttgattt
    32281 tgttttaatg ccgattgatc gatcgtcaat tcaacagtag ttgttttttc tttcgagcct
    32341 tcatagtaag tgattttgac agaatctccc acttttttct tatacaaagc agattgaagt
    32401 tctacaccag aagaaacttc tttgtcatcg atcttagtaa tgacgtcgta ttgtttcaat
    32461 ccagcttttt cagccggtgt agctgtttgg acatttgtca cgatgacacc gttagttaca
    32521 gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag aaacggcaga taagtcgacc
    32581 attgtgatac ctagagctgg acgtgtcact tttccgtctt tttccagttg attgatgata
    32641 ttcactacat cgttacttgg aatagcaaat cccattcctt ctacgcttac attagaagta
    32701 gattcagaag tacttgcgat cttacttgag ttgataccga taacttgtcc ttcgatatta
    32761 actaaaggac caccagaatt tccagggttg attgctgcat ctgtttggat cgcattgata
    32821 ttgactgttt cgttcgattc attcgtactt gtcacttgtc ggttcaagga agaaatgatt
    32881 cctgaagtga cagaatttgc atattcagat cctagtgggg aaccaatcgc aatcgcaggc
    32941 tcaccaacct tcagagcact tgagtcacca aaagaggcaa ctgtttcgac tttgtctgaa
    33001 ttgatcttca acaccgctaa atctgaataa gcatctgttc caactaattc agcttttacc
    33061 ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc catctactac gtgattattc
    33121 gtgactacat aagcagtatt cccgtctttt ttgtagatca cgccgcttcc ttcactataa
    33181 gcttccaaat tactatcatc tgagctgcta ctttcttctt gctgcccgaa tagtccgcca
    33241 aaaccgcttg attggttttg actttgtaag ttgataacag aaacgacagc gtcttgaact
    33301 ttatctaccg ctttggtgat atcagaatct acattgactt tgacattttc cacaactgtt
    33361 tcaccggcag aattctgatt gccgcttgta gcagtagaac tattgttgcc agtgcccatc
    33421 gctgcataaa aaatgccagc tgtcagtagt cctccaacga tccctccaac aagccccaga
    33481 cctaacttac gccagaggct attattttta ctttttttca tctttggtgt cacatttttt
    33541 ctatccatgt gttcctcctc caattacatt catttatcta ctgttagtat agtcttattt
    33601 gttgacttta gtctactttg agactgtgaa ctttttatga aaaactttag gaaaaatcat
    33661 tgttttcaga tgaaaatatt tgattttctt tttccacaga tactgtgtaa aactacgcgg
    33721 ataggattgg aaaaataagg gataaaacat atccacagcc tgtgtataaa gtggataaat
    33781 tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat aaggtaaaat aacactgttt
    33841 tctgtggata aacctgtgga tgttgtgcat aactgttggg gaaatctttt taacaaaaat
    33901 agccaaaata aacatataga cgaaaaaatt gattataata aacaaagtga gcatgaagac
    33961 caaaggagag aaaacatgaa tatcaaaatt atctcagtag gaaaattaaa agaaaaatac
    34021 ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg cctatgcaaa aatcgaattg
    34081 atcgaagttc ctgacgaaaa agccccggag aatttaagcg aagcgcaaat gagacaagta
    34141 aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag aacaagaata cgtctatgcc
    34201 ttagcaattg aagggaaaaa tcctacaagt gaatcttttg ctaaacaaat cgaccaatta
    34261 ggtatccaag gaaaaagcca ccttgttttt gttatcggcg gctctttagg actcagcgaa
    34321 gccgtcatga aaagaagcaa tgctcagatc tcttttggaa aaatgacgta tccacatcaa
    34381 ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct tccggatcaa tgcgggggcg
    34441 ccttatcata agtgatgcgg tttttatcat tgatcctaaa aatcttagca agacaaaaag
    34501 tacgtaagaa agtaaagact aaatacttcg gaggaacaaa tgaataacaa tgataggtta
    34561 ttgcgattgc gttatgcgat tgatttgaaa gatacggatt tgattaaagc ttttgagcta
    34621 ggcggtgtac cactgactaa agaagaagct caggcggtac tgaccaaagt acaggataag
    34681 aacaaagata atgcagaaaa taatgtgtat gaaaaaacca tcaacaacca agtttttgac
    34741 gcattcctaa atggcctgat tctcttggcg cgtggtccgc aaaaagataa gccgatagta
    34801 gacacgccgc aaaaaagcaa ggaaatcaaa tatatcaaca atgtgttgct aaaaaaatta
    34861 aaaatcgcct tgtctatgac cactgatgat attttagacg tttttgctga ggcagaaatc
    34921 tatccatcaa aaggcgaaat cggggcattt ttgagaaaag aaggccaacg aaattttaag
    34981 ccttgcggag ataaatatat gcgtaatttt ctcaaggggt taggaattta taatcggaga
    35041 aaagtataaa gaaatttcat gagaaatcaa aacaatggtt gttttgatga gagctagttt
    35101 ttgtatgaga ttaaaatatt atcttaatcg aggagctgga ttggttttat gaatgatgaa
    35161 tataagaatg atgaagataa gatgcttttt gaagagatag agaatcgatg taggctcaat
    35221 tttgagcttc ggggaaagat gtctttaata caacaaaaaa agtatttagc gaataaatca
    35281 gagtttactc tgggacatgt tgaaaaatta atctcggatt ggattagtag tagaagcgaa
    35341 tttacaaaaa taaaacagcc gattaaattt gacatgaaaa aacttttgtt aaataagagt
    35401 gagataggta atagagatca atatattaga gctaaagggc aagaaataat tgattcatta
    35461 ggtgaaatga ggtcatacaa ttatttgtat gtaacacata gagctgatgg aatggtcatt
    35521 actgtgggga aatcttcttc taatgatatt tttttagatg gagatctttt ttatcaactc
    35581 aatacaaatc acttgtctgg cactgaaaat ataattttaa ggacggaata tggcaatgaa
    35641 atatttgcaa aatatgatga gattttaaaa aattatttgg actgggcttg gataattccg
    35701 gttgagtcag gagatgctaa gaaactggag agattattgg gcgat
//
LOCUS       DAZKAO010000012        34960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000012 DAZKAO010000000
VERSION     DAZKAO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.012"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8H8_000477"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8H8_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP8985578.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8H8_000478"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8H8_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8985579.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8H8_000479"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8H8_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP8985580.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8H8_000480"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8H8_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP8985581.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8H8_000481"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8H8_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985582.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8H8_000482"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8H8_000482"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP8985583.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8H8_000483"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8H8_000483"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP8985584.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8H8_000484"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8H8_000484"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP8985585.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8H8_000485"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8H8_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP8985586.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8H8_000486"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8H8_000486"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP8985587.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8H8_000487"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8H8_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP8985588.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8H8_000488"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8H8_000488"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP8985589.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8H8_000489"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8H8_000489"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP8985590.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8H8_000490"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8H8_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP8985591.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8H8_000491"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8H8_000491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP8985592.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8H8_000492"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8H8_000492"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8985593.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8H8_000493"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8H8_000493"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP8985594.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8H8_000494"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8H8_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP8985595.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8H8_000495"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8H8_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985596.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8H8_000496"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8H8_000496"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP8985597.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8H8_000497"
     CDS             20771..21004
                     /locus_tag="ACG8H8_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8985598.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8H8_000498"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8H8_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP8985599.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG8H8_000499"
     CDS             21613..22026
                     /locus_tag="ACG8H8_000499"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP8985600.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG8H8_000500"
     CDS             complement(22064..23212)
                     /locus_tag="ACG8H8_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8985601.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG8H8_000501"
     CDS             complement(23334..23930)
                     /locus_tag="ACG8H8_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8985602.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8H8_000502"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG8H8_000502"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP8985603.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG8H8_000503"
     CDS             27160..27591
                     /locus_tag="ACG8H8_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985604.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG8H8_000504"
     CDS             complement(27636..28985)
                     /locus_tag="ACG8H8_000504"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8985605.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG8H8_000505"
     CDS             complement(29055..30218)
                     /locus_tag="ACG8H8_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8985606.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG8H8_000506"
     CDS             30347..30670
                     /locus_tag="ACG8H8_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985607.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG8H8_000507"
     CDS             complement(30853..32202)
                     /locus_tag="ACG8H8_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8985608.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG8H8_000508"
     CDS             complement(32271..33224)
                     /locus_tag="ACG8H8_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP8985609.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG8H8_000509"
     CDS             complement(33326..34516)
                     /locus_tag="ACG8H8_000509"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP8985610.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34960)
                     /locus_tag="ACG8H8_000510"
     CDS             complement(34716..>34960)
                     /locus_tag="ACG8H8_000510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985611.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9951 a         7642 c         6119 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagtgc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DAZKAO010000013        34864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000013 DAZKAO010000000
VERSION     DAZKAO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.013"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(516..2408)
                     /locus_tag="ACG8H8_000511"
     CDS             complement(516..2408)
                     /locus_tag="ACG8H8_000511"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985612.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2405..4132)
                     /locus_tag="ACG8H8_000512"
     CDS             complement(2405..4132)
                     /locus_tag="ACG8H8_000512"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985613.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4148..4798)
                     /locus_tag="ACG8H8_000513"
     CDS             complement(4148..4798)
                     /locus_tag="ACG8H8_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP8985614.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5002..5298
                     /locus_tag="ACG8H8_000514"
     CDS             5002..5298
                     /locus_tag="ACG8H8_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8985615.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5490..6110
                     /locus_tag="ACG8H8_000515"
     CDS             5490..6110
                     /locus_tag="ACG8H8_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP8985616.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6202..7368)
                     /locus_tag="ACG8H8_000516"
     CDS             complement(6202..7368)
                     /locus_tag="ACG8H8_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8985617.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7380..7733)
                     /locus_tag="ACG8H8_000517"
     CDS             complement(7380..7733)
                     /locus_tag="ACG8H8_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP8985618.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7861..8883
                     /locus_tag="ACG8H8_000518"
     CDS             7861..8883
                     /locus_tag="ACG8H8_000518"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP8985619.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8902..9063
                     /locus_tag="ACG8H8_000519"
     CDS             8902..9063
                     /locus_tag="ACG8H8_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985620.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9322..9756)
                     /locus_tag="ACG8H8_000520"
     CDS             complement(9322..9756)
                     /locus_tag="ACG8H8_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985621.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9866..11725)
                     /locus_tag="ACG8H8_000521"
     CDS             complement(9866..11725)
                     /locus_tag="ACG8H8_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8985622.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11726..11866
                     /locus_tag="ACG8H8_000522"
     CDS             11726..11866
                     /locus_tag="ACG8H8_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP8985623.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11843..13002)
                     /locus_tag="ACG8H8_000523"
                     /pseudo
     CDS             complement(11843..13002)
                     /locus_tag="ACG8H8_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13309..14733)
                     /gene="araA"
                     /locus_tag="ACG8H8_000524"
     CDS             complement(13309..14733)
                     /gene="araA"
                     /locus_tag="ACG8H8_000524"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP8985624.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14749..15447)
                     /locus_tag="ACG8H8_000525"
     CDS             complement(14749..15447)
                     /locus_tag="ACG8H8_000525"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP8985625.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15404..17065)
                     /locus_tag="ACG8H8_000526"
     CDS             complement(15404..17065)
                     /locus_tag="ACG8H8_000526"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP8985626.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17283..18683
                     /locus_tag="ACG8H8_000527"
     CDS             17283..18683
                     /locus_tag="ACG8H8_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP8985627.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18935..20014)
                     /locus_tag="ACG8H8_000528"
     CDS             complement(18935..20014)
                     /locus_tag="ACG8H8_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8985628.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20100..21059)
                     /locus_tag="ACG8H8_000529"
     CDS             complement(20100..21059)
                     /locus_tag="ACG8H8_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP8985629.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21071..22582)
                     /locus_tag="ACG8H8_000530"
     CDS             complement(21071..22582)
                     /locus_tag="ACG8H8_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP8985630.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22604..23167)
                     /locus_tag="ACG8H8_000531"
     CDS             complement(22604..23167)
                     /locus_tag="ACG8H8_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8985631.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23173..24036)
                     /locus_tag="ACG8H8_000532"
     CDS             complement(23173..24036)
                     /locus_tag="ACG8H8_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985632.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24050..24946)
                     /locus_tag="ACG8H8_000533"
     CDS             complement(24050..24946)
                     /locus_tag="ACG8H8_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985633.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25121..26410)
                     /locus_tag="ACG8H8_000534"
     CDS             complement(25121..26410)
                     /locus_tag="ACG8H8_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8985634.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26382..27899)
                     /locus_tag="ACG8H8_000535"
     CDS             complement(26382..27899)
                     /locus_tag="ACG8H8_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP8985635.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28097..28987
                     /locus_tag="ACG8H8_000536"
     CDS             28097..28987
                     /locus_tag="ACG8H8_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP8985636.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29060..29884)
                     /gene="nadE"
                     /locus_tag="ACG8H8_000537"
     CDS             complement(29060..29884)
                     /gene="nadE"
                     /locus_tag="ACG8H8_000537"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP8985637.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29896..31368)
                     /locus_tag="ACG8H8_000538"
     CDS             complement(29896..31368)
                     /locus_tag="ACG8H8_000538"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP8985638.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(32005..32367)
                     /locus_tag="ACG8H8_000539"
     CDS             complement(32005..32367)
                     /locus_tag="ACG8H8_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP8985639.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32590..33342
                     /locus_tag="ACG8H8_000540"
     CDS             32590..33342
                     /locus_tag="ACG8H8_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8985640.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33482..34405)
                     /locus_tag="ACG8H8_000541"
     CDS             complement(33482..34405)
                     /locus_tag="ACG8H8_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985641.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34420..34692)
                     /locus_tag="ACG8H8_000542"
     CDS             complement(34420..34692)
                     /locus_tag="ACG8H8_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985642.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10290 a         7246 c         6022 g        11306 t
ORIGIN      
        1 agccaaacaa aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca
       61 agcttttgaa caccgtttaa ggaagaggtt gtgaaaaagt cgcttagttt cgagtagtaa
      121 gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa
      181 actggctttt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tccgagtcac
      241 aaggagcaat ttcaaaaaac agcttctcag gtttgttgac tattgtgact tgtgaccgag
      301 gagttggcag gagaacaccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt
      361 attagagata caaaacggaa aaatagcttc tcatattttg ccgttttttg agctaatacc
      421 gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg actgtgacac gacttttgtc
      481 acagccccta aaaaaaataa taaatttgta ctattctatg aagcttcttc ttcagaaaac
      541 tgactgttat aaagatctgc atagaatccg tctttttcca tcaatgtatc gtggtttcct
      601 gtttctacga tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg tattgtagac
      661 aagcggtgag caacaacgaa acttgtccgg ttttccaata ggcggttcat cgctttttga
      721 atcagtattt ctgttcgtgt atctacacta gatgttgctt catcaaggat caggacatct
      781 ggatttgcta agaatgcccg cgcaattgtg attagctgac gctgcccttg cgaaatattg
      841 cttgcatcct cgtttaatac agtgttatat ccttctggca atttacgaac aaaatcgtct
      901 acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat
      961 ttgatattgt catagatact tcctgtaaac agccaagtat cttgcagtac cattgaaaac
     1021 tgtgcgcgca attcatcacg tgaaagatca cgggtatcta caccatcgta acgaatacta
     1081 cctccagaaa catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca
     1141 gtcggaccta caatagcgac catctcacca ggtttgacat ccaagttaaa atcagtcatt
     1201 agcaagttat ctggtgtgta gccgaattga acattttcaa aacgaacttt gtatggtgta
     1261 tctttttctt ctggaaggcc agatttttca ttggacatct cttcttcatc caacacttca
     1321 aatacccgtt cggctgaagc aactgttgat tggatagtgt tcatcaaact tgcaagttga
     1381 gtgattggtt gagagaactg tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc
     1441 atgttcccat ttgcaacttt tactccgcct agtacagcga cgaacacata gcctagattt
     1501 ttaacgaaat tcatcaacgg catgatgatt gccgaaataa attgagcttt ccaaccagcg
     1561 gcatacagat tctgattttc tttttcaaat acttcctggt ctttttcagt atgattgaat
     1621 gttttaacaa tggtatgacc accatatgtt tcttctactt ggttattcaa taaacctaaa
     1681 cttttttgct gatctgcgaa aaacacttgt gatttcggcg ctacgatcat cacgatgatc
     1741 aagcttaaag ggatcatcaa taacgcgact aacgtcaatt gccagctgat tgaaagcatc
     1801 atcacgatga ccccaatcaa tgtgactgca ctagttacaa attgagtcaa attttgctgc
     1861 agcgtgcttg cgatgttatc catatcattg attgctcgag acatgatatc cccatttgta
     1921 tgtgtatcat aataggaaat cggtacacga ttcattttgg cttccaaatc atgacgtaat
     1981 tcataaactg ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa gttgaataca
     2041 gcagaaatca aatacatcac gattactgtc gcaatgatct gcgcgatttt atcaaagtct
     2101 atcggaaatt ttgttacttg ttggccttgt tgcatctgtt gagcgccagc cattacacct
     2161 ttgaatattt ctgtggttgc tttccccaag acctttggtg tttggatttg gaagatcacg
     2221 gctgctattg ctagtacaaa tacagcgatg atcgcaacca tacgtttaga catatagcca
     2281 aacaatcggc gtaccgttcc ccagaaattt ttaggctttt caccttttac accgagattt
     2341 ctgccaggtc caccatgtcc tggaccgcct gctggtccat gagagggacg tgtgtttgtt
     2401 ttactcatgc taagtcctcc tctcttagtt gtgaatgaac gatttcttga taagtttcgt
     2461 ttgttgctaa tagttcttca tgtgttcctt tgccaaccag ttttccttca tctagcacaa
     2521 ggatcatatc tgcatccatt actgtactta ctcgttgagc aactaaaacg gtgatacttt
     2581 ctttcatatt cgttttcaat gcttggcgaa gattggcatc cgttttgaaa tccaacgctg
     2641 agaaggaatc atcaaacaca tagatatctg ctgttttgac taatgcccgt gcaattgcca
     2701 gacgctgtct ttgtccacca gagaagtttc ctcctccttg ttccacatgg ctatctaagc
     2761 catcttccat ctctgaaaca aaatctttag cttgtgcgat ttctaatgct ttccaaatca
     2821 tttcatctgt tgcatccggc ttaccatact gcatattgtc tcggatcgtt ccagagaaca
     2881 gaactgcttt ttgaggaaca aagcccatta attctcgcaa attatattga gtcatgtcac
     2941 taatgtccgt tccatttatt tggatcgaac cagattcgat gtcatataat cgagggatca
     3001 aattgactaa ggtcgtttta cctgaaccag tcccccctat gattgcgacg atctcgcccg
     3061 acttggcttg gaaatcaata tcttccaatg caagtttttc tgctccatga tatcgataat
     3121 tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt
     3181 cagggtcatg gataccgatt ttttcatcca acacttcatt aatacggtca gctgaagctt
     3241 gtgcacgagg caccatgata aaaatagcgg aaagcatcat aaagctgatc aagatctgca
     3301 tcgcataagt cataaatgca atcagattac cgacttccaa agtcatatct gctatgtagt
     3361 gtccaccaaa ccaagtgatt gctacgtttg taccgctcat gatcaatgtc atcattggca
     3421 tcatcaatgc gacgatcgta ttaactttga ttgccgtatt tgtataatct ttattcgcta
     3481 aatcaaaacg attttcttca aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg
     3541 tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt cttttgcata cttttgaata
     3601 atggaacagc aaagtacatg attccgccga tcaaaataat cattactggg atgacaacta
     3661 agaagatttt tgtcaactga tgatcttttt gataagcaag tatgctcgcg ccaatcaacg
     3721 tgattggtgc attgatcatc atacgtaaaa acatttgtgt aaccatttgg atttgagtca
     3781 catcattggt cgttcttgtg atcaaagagg ccgttccata tttatcaaac gcatcttttg
     3841 tcagactctc tgatttttta tagacatccg tacgcaattg cttacccagt ttctgtgatt
     3901 cacgagtagc aaaaaatgta tttgcgatag cagctaagat actgatcaaa gaaaagccga
     3961 tcatcacaaa acctgtatgc caaatataat cgacatctcc ttgcgctacc cctttatcaa
     4021 taatattgga ggtaagtgtc ggcaaatata aatctgccag tacttggatc accatgaaaa
     4081 ctgcagctgc aattgctgaa gtcaaggaga tccgttttac gatttttagc atctagttcc
     4141 cttctttcta tttatatact ccgtttttca gccaagaaag tttcaaattg aattcactga
     4201 ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg
     4261 ttgtaaatac agtattcatc actaattgga tcagtaattc caattcatga tcatttttta
     4321 ctttcagctt cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt
     4381 tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt cttgaggcac
     4441 ggtaatccat gttcataaac aagttacgat aaaaagaagc attctcgcct tccaaaatct
     4501 cataaatcat tttagaaaaa taggattcga atcctttgaa tagatcacca tcggattctt
     4561 tcattagttg taacatatct ttagagctgt cacgtctcat tgtctcaaag taataaaaat
     4621 aaagatcctc tttatcttca aaatactgat agaaactgcc tctagggata tccgctaatt
     4681 tgacgatatt agcaatcgaa gcttctttta agggtactct agaaaactcg attctcgcag
     4741 cctcgattag tcttttttgt ttttctttag gcaaatggaa aaatgtctgt gtcggcatat
     4801 ggattctcct ttctttcaag atgacacatt gtcatatgac gctatgtcat tataacacct
     4861 cctacctgtt ttgcaagaca tttgcatagt ttttttcatt atcatccgat acattaaaaa
     4921 agtgataaga ggaatccttc catttgaatt aatgagagtt gcccattaaa atagccacat
     4981 actaaacaaa ggatgagata aatggcccaa tcaattagtg tccaagactt caataaattg
     5041 cctctcgatg ctgacagcat cgttttagat acaagaaata ccttagatta cacaaaaaac
     5101 cacttaagcc actcgctttc tatccccgct gctatcttgc caagacaatt aaaaaatcta
     5161 gatccaaaga aactttatta tattctaagc tatacaggca ggcgatcaga agcattagcc
     5221 gatcaactta ctgctaatgg ttttcgtgct gtttatatca ttggtgggat ggaagctctt
     5281 cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa gaggctgtga caaaaaatcg
     5341 tgtcacagcc tcttttatac cctgtctttt tttagttatt tattcttcaa ccttatcaat
     5401 atgacttgat agccttttct ttttcgagta aactgatttt gttagtcata gttataacaa
     5461 tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc
     5521 ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata
     5581 aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg
     5641 aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt
     5701 ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc
     5761 ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat
     5821 taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg
     5881 ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat
     5941 acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg
     6001 atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt
     6061 tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat
     6121 aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg ccttggatat
     6181 tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag
     6241 agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata
     6301 aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac
     6361 taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa
     6421 gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg
     6481 acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg
     6541 gatcaggttt aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac
     6601 cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat
     6661 ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg
     6721 tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa
     6781 taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact
     6841 tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat
     6901 gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa
     6961 aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct
     7021 cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac
     7081 aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta
     7141 atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac
     7201 gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac
     7261 gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca
     7321 gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt
     7381 tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag
     7441 attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct
     7501 actgtcttta aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca
     7561 tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga
     7621 atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt
     7681 tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc
     7741 tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat
     7801 aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat
     7861 atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg
     7921 atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat
     7981 agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat
     8041 ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag
     8101 tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg
     8161 aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca
     8221 gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat
     8281 gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat
     8341 cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact
     8401 gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat
     8461 ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct
     8521 tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg
     8581 gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac
     8641 tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc
     8701 tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg
     8761 tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta
     8821 ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt
     8881 tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa
     8941 ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat
     9001 cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac
     9061 taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg
     9121 atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat
     9181 ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa
     9241 ggattttgat cactctatca taaaggtaat attacattta tattagataa ctctagtttt
     9301 tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc
     9361 agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc
     9421 atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga
     9481 aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt
     9541 actgactttc ataagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat
     9601 ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat
     9661 cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc
     9721 gatggactgc acataatagc caatattcgg taacatgttc gttttcctac tttctccatt
     9781 gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta
     9841 atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat
     9901 attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt
     9961 gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt
    10021 caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct
    10081 gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag
    10141 tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg
    10201 tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc
    10261 aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg
    10321 ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat
    10381 ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct
    10441 gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt
    10501 ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca
    10561 tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat
    10621 acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg
    10681 aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt
    10741 gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc
    10801 caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat
    10861 aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac
    10921 tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat
    10981 cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag
    11041 gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat
    11101 agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca
    11161 acaaattatc gggattgttg aaattactga tataccctag cagtccaatt gccacaagca
    11221 gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc
    11281 agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc
    11341 cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg
    11401 gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga
    11461 catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa
    11521 tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca
    11581 acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa
    11641 caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg
    11701 aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg
    11761 ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat
    11821 gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc
    11881 gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac
    11941 tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga
    12001 tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt
    12061 gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga
    12121 ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc
    12181 cgctgtctgc caaagcttcc tgagtcgatc ccctagatga ggcaacataa gtatacggtc
    12241 attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat gttgtttttc
    12301 caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat tgataagtac
    12361 agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg tatcttctgt
    12421 caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta aatctgcttg
    12481 ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca tccctaagct
    12541 atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac cgatggtttt
    12601 gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt ctaaaatatt
    12661 aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg aagataagag
    12721 caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg tattcatgac
    12781 gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa caccagctct
    12841 tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac tacgaataac
    12901 tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta acttttctat
    12961 ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc ctccttacta
    13021 acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt cctagaagca
    13081 aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga caagttactg
    13141 ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat aaatggtgtt
    13201 ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc
    13261 aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt atctaataac
    13321 cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg ttagatttag
    13381 cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac cttcatggaa
    13441 attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg
    13501 ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat ctgccatcga
    13561 tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat cttctctatc
    13621 tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg tcggatctac
    13681 ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt aagtataatc
    13741 ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat caatcgctgc
    13801 agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac gttgtacagc
    13861 tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata
    13921 gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta cttttacatg
    13981 ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt ccttgtactc
    14041 ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta caagatctcc
    14101 gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt tgtctccttc
    14161 tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc caaaactttc
    14221 attataggct actgcaacat ccatccaatc agcaatctct ttttggatct caggtcgttt
    14281 ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga acccatattc
    14341 gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg ttttccaagg
    14401 aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa gctttgtccc
    14461 gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac ctgctacttc
    14521 ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc
    14581 ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga ccatttcttg
    14641 tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg atcctacaac
    14701 aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt atttgttttg
    14761 tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa tccgttgagg
    14821 cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc atacttcatc
    14881 tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa atggaccgtg
    14941 tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc
    15001 tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa
    15061 tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac
    15121 atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc
    15181 gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag agggattgag
    15241 tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc
    15301 ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat caaaagcgct
    15361 cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta
    15421 tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc aattgatgaa
    15481 tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc tgatagcctg
    15541 ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac acgtcttctg
    15601 ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct aataacgcga
    15661 ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc tccatagcag
    15721 atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc cccactaaac
    15781 gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt gcaccaaaag
    15841 cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct ggtgtacgta
    15901 caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa taaccataag
    15961 aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat accgtgaata
    16021 atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa atactgaccc
    16081 aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt tctcctgaag
    16141 gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct aatacgatca
    16201 tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc tttggctaca ctgttcgtag
    16261 cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct ggtctcaaat
    16321 ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct tcttctcctg
    16381 ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta
    16441 actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga tcgataggaa
    16501 acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc caatggacat
    16561 aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta ttcaaaatag
    16621 attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat ttttccgtta
    16681 atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt actagtaact
    16741 gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca atcgacccga
    16801 ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt cggtaactga
    16861 cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt tctagtttgt
    16921 tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc aatacagctt
    16981 tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg acatctaaca
    17041 tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt caaacctgat
    17101 caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac gtacatgtca
    17161 agcagttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat aagttataaa
    17221 atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag gagagaatac
    17281 ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc gcatttggcg
    17341 gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt ttacagcatg
    17401 attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg gtcatgttag
    17461 gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga cgacgaaaaa
    17521 tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca tctgcccctc
    17581 acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct gttggcgctg
    17641 catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta aggggaagat
    17701 tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat gttgcagatt
    17761 atttgctaaa agggttgcct gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg
    17821 tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg cgttttctta
    17881 tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag
    17941 aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag acaaccggaa
    18001 cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc ggtattggtg
    18061 tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt ccgctgatcg
    18121 tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgatc atccaaggaa
    18181 tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc aatcggcgaa
    18241 ctttattgaa aataggcggt agcgtgatgg gactttcttt catcctacca gctgttttag
    18301 gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta tgtatttatg
    18361 tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcgtaggc gaaatattcc
    18421 cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg attggttctt
    18481 tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa atcgttttcg
    18541 gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc gtaccagaaa
    18601 ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac
    18661 gtatttctat ccatcattct taaagaggtt gtgacagaag tggtcagctt caagaaataa
    18721 gaaggaaccg cttagtttcc agtcacaagg aacaatttca aaaaacagct tctcatgttt
    18781 gatgaatatt gtgacttgtg accagttggt aggagaacac cgtttagtgg agaggtggtg
    18841 aaagaaacgt tcagccttga gcgatgacaa aagctctcac aaacattgcg acgagtaacc
    18901 gtaggagcaa taagaggcgg taaaaacccg tctcttattt ttcgttttct ttcactgttt
    18961 cccgttcaac tagtacaggt tcatagagaa tatttggctg tgcctgtcct ttttcaatcg
    19021 ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg
    19081 tcaatttttt attccctaaa tggcttaaag aagaatcgtc tgttcctacg atcgataggt
    19141 catctggaat atgatagcca ttttctgtca acttatctag cattaaacta gctatttgat
    19201 cgttatagca aatgatgcca tctaaagctt gttcttcttt caaccgttgt actgtttttt
    19261 cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat atcttccgtc aaaaaacgca
    19321 cattatactg ttcacacgcg ctgataaaac ctttcatcct gtattttcct tgaagatcgt
    19381 cgatttttgt gatgaacaac agacgctgat gtcctttttt tatcaaatat tctgctgcca
    19441 taaaaccgac tttcacatca tctaaacaaa tataagaggc atttaactct tcataagcag
    19501 cattgatcat caccatcgga atgccttgtt ctctcaaagc aacataaagt gctaagtttg
    19561 ggttatactg attacttttc gtaggctcaa cgatcaaacc atctacccca taagcaatca
    19621 ttttcttcaa acactctttc tccaattcgt gatcgttatt cgtactggct aaaagcaatg
    19681 aatacccaga ttctcgtaat tcattttcga ttcctcggat aatcgacgga aaaatataat
    19741 cagagagata tgtggtgatg acaccgatcg tttttgttct gttcaatggt gttttttctt
    19801 tcttatagct gtcatctaca tatgtgcccg accctttttc tttacgcaga tagccctcat
    19861 ttaccaacac agcgattgct tgccgaatcg tatgtctgct gacgtcatat atttcttgca
    19921 gcttgaattc tgagggaatg gttgtattta cttcgtattc ctcacgaagg atcttttgtt
    19981 ttatctcttc tgcaatatct tgatattttg ccatttgcct tgcccctttt tatttgtacg
    20041 tacatatatc ataacgagtc tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct
    20101 cataaggcat acggaacagg ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag
    20161 cattgcgcgt tcgcgtgccg attaggattg tctaacggat cccctttttg gttcttctct
    20221 ggtcgagcat gatagatcaa agtatcaaag cacccgtctt ctgaaaccgt aaacgagttg
    20281 tgaccaggac caaattgctt atttttttca gaagaaacga atactggctc cgataattta
    20341 tgccatgatt cttttaataa aagattcttg tgctcatcag cccacagcag gcccatggca
    20401 tagttttcat ctgttgcact accagaatac gtaatgaata ttttcccatt gcgagtaagg
    20461 actgctggac cttcatttac tgaaaaaccc cgtttttccc attcatactc aggtatagaa
    20521 aggaggatct gcttcccctt taacgtccaa ggattatcca tttcggatag ataaagatta
    20581 gaattcccag taatttcagg atcttgttgg gcccacacat aatatagctt tccttgagaa
    20641 gagaaagttg ttccgtctaa actgaaagtt tcccttttcg tttttatttg tcccttttct
    20701 atccaatctg tattcattgg atctatatgc tgattttcaa gtacgaacat gcggtgatga
    20761 tgagattcat cacgtatagt tttatgattg cttgcagcaa agtaaatata ccattttcca
    20821 ttgatataat ggacttcagg tgcccagatc aattcactca tcggtccttc ttcatgagcc
    20881 tgccatacaa tcaggcttgc agcatgttct aattcattca gtgactttgc ccttcttaat
    20941 tcaatcactt ggtagcctgg aaccgatcct gtaaaataat aataaccatc tgtatgttta
    21001 tatacccaag gatctgcccg ttgccgaacg ataggattat tataaagtgt cttcatcata
    21061 acaaatttcc tcacattttc tttattctaa ttacattcca agagtatttt tctatcccta
    21121 catttaattc gtcatgtttt tctattgaaa aatagtttat ttcaggtttt attttttctg
    21181 gttcttcacg tgtatttttg tccaacagat cgctactggt taatgtgtag tgctctcctt
    21241 gatctagcaa attgaaatca tgttcaatcg ttatcttttg ttgttcattt gtgcgattga
    21301 tcaagaaaat agtcagctga ttgccgttat ctacgatcac actgtccaca taaggaacat
    21361 tttttatctt ttcacaatca taaacagggc aaatgatttg ttgtttcaat acttgacctt
    21421 tagcatattt cgataagtgt tgcatcacat aataagttgg attcctccat gcttgtcctt
    21481 tagcatctgt cagtattaaa ggtattgtgt ttactaacaa tgattgacaa ctaatcttta
    21541 cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc aaataataat gtatcttcca
    21601 tagtaaagta acaccaatcg atgggactac cgacttcaaa atccctatgt cttttttccg
    21661 gttgggcatg tacattccat tcatcaaacg ctagatacat tgttttctca cttctcaaat
    21721 atccttttac ataatcacaa gtagcaacga tttcttcaat ttgacgatcg aatcgataag
    21781 cttttgcaag gtatgtcgct gtatcatcac gctcattttt aggaggttta gtcaagtctt
    21841 cttcatcata cttatcaata tagttatgca aagctaaata atctacattc tcataagctt
    21901 cttctaatgc gatacgatcc cattcagggt acgatggtaa acgtggcgta gagcttccaa
    21961 ctaagattgt ttcaattgaa tcatccacta acttcatgac ttttgaggct tcatttgcta
    22021 acctaccata ttcataagca ctcttagccc ccacttgcca tggaccgtct agctcattcc
    22081 ctaaacacca taatttaacg ccaaaaggct ttttttgccc attttttatt cttaaatcac
    22141 tccagaaggt tccttttgga aaattacaat attctactaa attttttgct tcatcaattc
    22201 cttttgttcc taaattcacc gtgaatatcg gtttcgcttg tacatgactg atccatttaa
    22261 aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg ccatgctaaa tcaatttttt
    22321 ttggtcgttg ttctttccta cctacagtat ccacccaatc ataaccagat gtaaagtttc
    22381 ctccaggata gcgaatgaca cctaattcta attcctttac ataagagagg acatcttgtc
    22441 taaacccgtc accatcagct gtttcatggc ccggttcata aatccctgta tagacgacac
    22501 ttcccatatg ctcaacaaat gagccaaaca gccgagaact gatctctcct accgtaaaat
    22561 cctctctgat ttttactttc acttcttcgt ccaacagctt tccctacctt tcatttccta
    22621 tagtcaagat aatagataca agtttccata cgccaccagc aaagataaaa ataaaaggag
    22681 ggaaaagaaa tatgagcagt acaaatccta ataacaatac gcaacaaaga atatttctca
    22741 aaaaatcaaa aaatactttc catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt
    22801 tgttttcaga aatcacatat atccaggata aaagaaacat ggaataaata agtaaaacta
    22861 catatccact aattgaaagt acgattgtct gttcttctaa taattgatta acaagaaaat
    22921 aaataatgat cgttaacaga ctaaggaaca agttcttacc aaaatgttgc tttattcctt
    22981 gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc attacataaa
    23041 aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt acccagagaa
    23101 tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta gtctcaacta
    23161 ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa aatatttctg
    23221 tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag ccattacagg
    23281 tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta aagttctcat
    23341 ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat tcatcagcgt
    23401 gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt tcaaaaaaat
    23461 attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat
    23521 aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa tgatcaaacc
    23581 tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa tcatgaagac
    23641 ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa
    23701 acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag tgatcactgt
    23761 ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg ttgtccaaac
    23821 cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa atgagataat
    23881 gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg caataaccaa
    23941 caatataact agcatactaa tcgttcgaat gattttttta ccactaactg cttttcgact
    24001 attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc tatttcattt
    24061 gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca acaatgataa
    24121 acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta aatcctcgac
    24181 tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg gttgcaacat
    24241 atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa aaacctaagg
    24301 agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca
    24361 tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag agaatcgtca
    24421 tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata gcaagggttg
    24481 gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc
    24541 ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa
    24601 ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta gataatgcta
    24661 ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg aaatataaag
    24721 aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac
    24781 ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata gcaaatgttc
    24841 ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt aaaaacaaat
    24901 acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag tccacctcat
    24961 ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag atatccgaac
    25021 aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc attcagatat
    25081 ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc agtaattgat
    25141 tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct aataattttt
    25201 caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa cctggtaaat
    25261 atggctctgc atagtccatt tgtttcgata attcagatac aataggatca ttttttactt
    25321 cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata ctccattttt
    25381 taccaatttc agttgtattc caatatttca aaaactcata gacagcttct tttttattat
    25441 tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt tttttgtctg
    25501 aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct ttcaatccgt
    25561 tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt aaattgtcca
    25621 tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt ttttggatcg
    25681 cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa tcatcgttca
    25741 ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca tccttattga
    25801 agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt tctgtcaatt
    25861 cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac aaatcattat
    25921 tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta ccttctactt
    25981 cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca tattcccaga
    26041 aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct ggataattta
    26101 atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt tcattcaaat
    26161 tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt ttattaaatt
    26221 cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc caaaaagtca
    26281 aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa
    26341 tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc attttttgtt
    26401 cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt caataaacaa
    26461 ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac ttgttcactt
    26521 ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc aatcactccc
    26581 tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc acggttaact
    26641 gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata cggcacttct
    26701 tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt
    26761 ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc ttctccgttt
    26821 gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc aatctttact
    26881 ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc atcttcaaaa
    26941 ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg ctcatcttgt
    27001 tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg cttcttacta
    27061 tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa ccgatccatg
    27121 tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata atattgatgc
    27181 agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc ccaatcacca
    27241 aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga atcatctacg
    27301 agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc tgttttatgt
    27361 ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat tccaaaaggg
    27421 ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg aaaattacaa
    27481 tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac agccatattg
    27541 atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac ttcattcgtt
    27601 tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg cccaatactg
    27661 tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat gggaatgttc
    27721 aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc tgatggatga
    27781 tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa tgaactgtac
    27841 aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt ggcttccata
    27901 actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt gtcaataata
    27961 taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt aataatgatt
    28021 aaaacaaata atttaatata acttttatta acaaatatgt taatatataa gaaaaacctt
    28081 ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg tatatgaagc
    28141 attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa agatgaatgt
    28201 caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa
    28261 gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct
    28321 tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt ttgatacgaa
    28381 agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa catgcggttt
    28441 agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta tggatcctcg
    28501 aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc aggcacctaa
    28561 ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa tttcttctga
    28621 atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa atggtgtgga
    28681 gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat atacgcctga
    28741 ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa ttaccaaaca
    28801 cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca tccctaaaga
    28861 gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg tggggggctg
    28921 tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa aagtttacta
    28981 cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct atgaagggac
    29041 ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt ggcaaatgtc
    29101 gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga gcgggaactt
    29161 cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg acttcatctg
    29221 caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat aatttttccg
    29281 gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg aataatggca
    29341 aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat
    29401 gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc gtaatcatac
    29461 gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct gcttcgctta
    29521 atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta acatcgggct
    29581 ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca taaggaagac
    29641 gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct tccatcgtta
    29701 gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct agtacaaaag
    29761 ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt ttacgtattt
    29821 cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct gtctgcaggc
    29881 tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt tcattcgttc
    29941 catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt atttttgcgg
    30001 attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg tatattcttt
    30061 gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga tatcttgtaa
    30121 aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca ctggatggaa
    30181 catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt agtctccttc
    30241 agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg gagtagttac
    30301 tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat catcttcgat
    30361 cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga tcaattttgt
    30421 cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc
    30481 atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat ctaattgttc
    30541 ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc cttggaagtt
    30601 gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg atttcaacgt
    30661 atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg ccataaatgc
    30721 ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc tagctggtat
    30781 cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga tatatgccgc
    30841 tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa attccaatag
    30901 tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca acgtttggaa
    30961 attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag gcccttccac
    31021 ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac gaactgttgc
    31081 tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt ctaatccacg
    31141 caagtaagca atatcggaat cattgaacgt cagattctct aaataattga ctaaccgttc
    31201 caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa
    31261 aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat tgatttgata
    31321 tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct tgtattactc
    31381 ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag gaaaagaaat
    31441 cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa aatgaactcc
    31501 atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga atacttattt
    31561 tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta cttgataaaa
    31621 taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga acaaatataa
    31681 ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat acgtctttcc
    31741 ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga atttttttgg
    31801 aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca
    31861 cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa caaaaatggc
    31921 gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg agcaggttac
    31981 cataaagatg attattagtc gttattaaat agaatccttc gtattttttt gttctatcac
    32041 tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct cctgtttcac
    32101 tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc
    32161 gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct ttttatcata
    32221 tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat aattaatagc
    32281 cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat ttaaattact
    32341 attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt cgatttagat
    32401 ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac aattgaacct
    32461 ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt cgtgagaaaa
    32521 aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca tttgtcagga
    32581 ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt cagtccgaga
    32641 accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa ttcttcaggt
    32701 tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt ttatcgaaca
    32761 atggcattca agttggatga tatccaagag gtcttagaca atccatcttt tgaccaccag
    32821 aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat cgataccctc
    32881 ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat gagtgacaaa
    32941 gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa
    33001 gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat
    33061 atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt atttgatctc
    33121 actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt ccaagctcat
    33181 aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa aggactagga
    33241 atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag tggtcatggt
    33301 gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac atgatccaac
    33361 aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt tagattttta
    33421 tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac tttaaatata
    33481 ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct gtaagaaaca
    33541 attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg aaaccaagaa
    33601 gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc
    33661 tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca actccaagct
    33721 ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc caaaaaacaa
    33781 gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt tttgagttat
    33841 aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct tgctgcttca
    33901 aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag cttgcctgtt
    33961 ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc aataaatcat
    34021 ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct gcaaattctt
    34081 caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc actttgtaat
    34141 cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac ggatgattgg
    34201 tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt ctggcatttg
    34261 cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt ttttcctcat
    34321 ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa cctgtgagct
    34381 ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa gattttcttg
    34441 taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc cttcatgcga
    34501 aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga tttcattcat
    34561 tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga
    34621 tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg ttatctctac
    34681 ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg aaacgcgtct
    34741 catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt attaccaaag
    34801 tctcttattt taaattgaat acattataat gatactgtaa atatatcttt attattagtg
    34861 aatg
//
LOCUS       DAZKAO010000014        34467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000014 DAZKAO010000000
VERSION     DAZKAO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.014"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..2075)
                     /locus_tag="ACG8H8_000543"
     CDS             complement(33..2075)
                     /locus_tag="ACG8H8_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985643.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(2065..2832)
                     /locus_tag="ACG8H8_000544"
     CDS             complement(2065..2832)
                     /locus_tag="ACG8H8_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985644.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3158..5347
                     /gene="nrdD"
                     /locus_tag="ACG8H8_000545"
     CDS             3158..5347
                     /gene="nrdD"
                     /locus_tag="ACG8H8_000545"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP8985645.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            5662..6264
                     /gene="nrdG"
                     /locus_tag="ACG8H8_000546"
     CDS             5662..6264
                     /gene="nrdG"
                     /locus_tag="ACG8H8_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP8985646.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(6318..7439)
                     /gene="dinB"
                     /locus_tag="ACG8H8_000547"
     CDS             complement(6318..7439)
                     /gene="dinB"
                     /locus_tag="ACG8H8_000547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP8985647.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDYHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(7548..8420)
                     /gene="rsmI"
                     /locus_tag="ACG8H8_000548"
     CDS             complement(7548..8420)
                     /gene="rsmI"
                     /locus_tag="ACG8H8_000548"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP8985648.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(8489..8836)
                     /locus_tag="ACG8H8_000549"
     CDS             complement(8489..8836)
                     /locus_tag="ACG8H8_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP8985649.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(8829..9653)
                     /locus_tag="ACG8H8_000550"
     CDS             complement(8829..9653)
                     /locus_tag="ACG8H8_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP8985650.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            complement(9689..10627)
                     /gene="holB"
                     /locus_tag="ACG8H8_000551"
     CDS             complement(9689..10627)
                     /gene="holB"
                     /locus_tag="ACG8H8_000551"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP8985651.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(10641..10970)
                     /locus_tag="ACG8H8_000552"
     CDS             complement(10641..10970)
                     /locus_tag="ACG8H8_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP8985652.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(10985..11629)
                     /gene="tmk"
                     /locus_tag="ACG8H8_000553"
     CDS             complement(10985..11629)
                     /gene="tmk"
                     /locus_tag="ACG8H8_000553"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP8985653.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(11693..12289)
                     /gene="recR"
                     /locus_tag="ACG8H8_000554"
     CDS             complement(11693..12289)
                     /gene="recR"
                     /locus_tag="ACG8H8_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP8985654.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(12314..12628)
                     /locus_tag="ACG8H8_000555"
     CDS             complement(12314..12628)
                     /locus_tag="ACG8H8_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP8985655.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(12648..14393)
                     /gene="dnaX"
                     /locus_tag="ACG8H8_000556"
     CDS             complement(12648..14393)
                     /gene="dnaX"
                     /locus_tag="ACG8H8_000556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP8985656.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            14608..15855
                     /locus_tag="ACG8H8_000557"
     CDS             14608..15855
                     /locus_tag="ACG8H8_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP8985657.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15880..17064
                     /locus_tag="ACG8H8_000558"
     CDS             15880..17064
                     /locus_tag="ACG8H8_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP8985658.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            17061..17858
                     /locus_tag="ACG8H8_000559"
     CDS             17061..17858
                     /locus_tag="ACG8H8_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP8985659.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(18300..18956)
                     /locus_tag="ACG8H8_000560"
     CDS             complement(18300..18956)
                     /locus_tag="ACG8H8_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8985660.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            19149..19427
                     /locus_tag="ACG8H8_000561"
     CDS             19149..19427
                     /locus_tag="ACG8H8_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985661.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            19446..20654
                     /locus_tag="ACG8H8_000562"
     CDS             19446..20654
                     /locus_tag="ACG8H8_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8985662.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(20802..21167)
                     /locus_tag="ACG8H8_000563"
     CDS             complement(20802..21167)
                     /locus_tag="ACG8H8_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP8985663.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(21314..22087)
                     /locus_tag="ACG8H8_000564"
     CDS             complement(21314..22087)
                     /locus_tag="ACG8H8_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8985664.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(22261..23121)
                     /locus_tag="ACG8H8_000565"
     CDS             complement(22261..23121)
                     /locus_tag="ACG8H8_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP8985665.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(23121..24539)
                     /locus_tag="ACG8H8_000566"
     CDS             complement(23121..24539)
                     /locus_tag="ACG8H8_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP8985666.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(24596..25045)
                     /locus_tag="ACG8H8_000567"
     CDS             complement(24596..25045)
                     /locus_tag="ACG8H8_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8985667.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(25490..25753)
                     /locus_tag="ACG8H8_000568"
     CDS             complement(25490..25753)
                     /locus_tag="ACG8H8_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP8985668.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(25852..27147)
                     /gene="rho"
                     /locus_tag="ACG8H8_000569"
     CDS             complement(25852..27147)
                     /gene="rho"
                     /locus_tag="ACG8H8_000569"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP8985669.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(27144..28415)
                     /locus_tag="ACG8H8_000570"
     CDS             complement(27144..28415)
                     /locus_tag="ACG8H8_000570"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP8985670.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(28581..29450)
                     /locus_tag="ACG8H8_000571"
     CDS             complement(28581..29450)
                     /locus_tag="ACG8H8_000571"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP8985671.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            29553..29765
                     /locus_tag="ACG8H8_000572"
     CDS             29553..29765
                     /locus_tag="ACG8H8_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985672.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(29742..31352)
                     /locus_tag="ACG8H8_000573"
     CDS             complement(29742..31352)
                     /locus_tag="ACG8H8_000573"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP8985673.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(31574..31819)
                     /locus_tag="ACG8H8_000574"
     CDS             complement(31574..31819)
                     /locus_tag="ACG8H8_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985674.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(32545..33885)
                     /gene="glnA"
                     /locus_tag="ACG8H8_000575"
     CDS             complement(32545..33885)
                     /gene="glnA"
                     /locus_tag="ACG8H8_000575"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP8985675.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(33913..34305)
                     /locus_tag="ACG8H8_000576"
     CDS             complement(33913..34305)
                     /locus_tag="ACG8H8_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8985676.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT        10040 a         7244 c         6116 g        11067 t
ORIGIN      
        1 gttctattta ttatcttgtc ttattccgta tatcattcct cgataatccg acaatataac
       61 tgactcgtaa tgacataaag caaaagatag ataaggatca ataagccact cacaagataa
      121 gccaaccagt aatcggcagt ttttatatac tgagtgaaga cattgattgc aaatactgcg
      181 tgagtgatcc ctaatatcat gggtggaaag aaggtaagtg cgttttgctc gaagatcatc
      241 cgtctgatcc ttctttgtgg aatccccagc ttagtcaata ggtgataatt ttctttttct
      301 tcctctgctt ctgctaattg ccggactgtc aaaatactac cagtagcaat aatcacgatc
      361 atcccgacaa atagtgtgac aaaaatattg ataccgattc gccgattaac tgcacgcaat
      421 ttcgaatagc tggaagagaa attcaaacga gagctttctt ggctaaattg tgcttccttc
      481 atttggtcat ccggaagttt ctttgtgcta atttctcctt gtagacttcc atttacgata
      541 tcataagaat aataaacagc attgagccac tttatcgaca ggtcattgct cagtcgttca
      601 tctatttttt cattatttcc acctgataca tctacattga ctacttgata atccacacct
      661 tttgcttttt caaaaacggt atcatctaca atcagtacag gacttagata taccattgtt
      721 tcgtcaccta atacacttgg aagcatttgt tgaatcttca attcttgatt atctggcagt
      781 ccaatactag atccatataa tgaaatattc cgaaaaagag tttgcatttg atcaagcaaa
      841 acagtatgac caggtgattt caaagaaatg ttcggcaaat ctggattaac ttttctgaag
      901 gcttgatatt gtgtttccgt gatcagatta gcagtttgaa acataggttc ttcgagattt
      961 tgactataga tactcccaat gactttataa tgcaaggtga tttctttagt tatcttctgc
     1021 ccttcctctg ctagaatcgg cttgattttt ttctctgtct caacatcaaa ctgatagtcc
     1081 atcggggagg aaactttctc ctggtaggat aaaatagtag agatactagc cgtgccgcca
     1141 attgtcatta aagaaattcc gagaatgact gtaatcagag aatttgtacg ccaacttttg
     1201 agcaaatgaa cttgcgtgtt acccattatc aaaaaattga taccatagta ataaatcttc
     1261 catttactca atatgatcaa aaataaccga aatgtataac agaaaaacaa ataagtaccg
     1321 accacacaaa cgaaaaaaat taaaaaagga aaaaagaaaa ctaatgtatc aagataatga
     1381 taactgatca tccatgaagt gatttcccta aaatgactag ctccaaaata tccgctcgtt
     1441 aaaagtagta acccgatgac tgcgagtaat cggtgccttg tcctgaagcg cattgttgaa
     1501 ctatctgctt gttctttttc tccaaaactt cgaatcattg gatagcgcca tattttccat
     1561 attgaatata cagatactgc taaaaggatg atgaaaaaag caaataaagt atctactact
     1621 gatggaatgg agataaaaaa tggactagta agatccatat ccatcattcg aaccaaaatc
     1681 attgaaaaga gtttggaaaa aataataccg agtaatatcc cagaaataca ggcgaacaat
     1741 ccaattatca tgatttctaa cacatatata cctgatatct gcagtttatt cataccaaaa
     1801 agctgataga tgcctatttc cttttgcctt ctattcaaga aaaatcggtt ggcactgaat
     1861 acgaaaaaaa gcaaaacaac agtaatgata aatccaccaa aactcaatcc accatctatc
     1921 gaagtatctt gacttgctct tctgactaaa gattgatcgt aggtcattgc gctgaaggaa
     1981 taatagatca ttacggataa agtcaaactg aaaaaataaa caagatagcc tcgtgactgc
     2041 cgaaaaaaac ttttaacggc tagtttatac agcattcatt tttcctcctc cgccaatcgt
     2101 cgcctgcatg tctattactt tctcaaagaa ctctcttctt gtccctcttc tgacaacttc
     2161 agaaaaaatc acaccatctt taataaataa aattctatcg cagtagcttg ctgtatatgg
     2221 atcatgggtg actaacagta tcgttgcttt ttctttttga ttcatggtct tcaagtagtt
     2281 caacaattct gtagccgact tcgagtccaa agagccagtt ggttcatctg caaaaatcac
     2341 ttgtggctga acaacaagtg ctctagcagc tgcaattcgt tgtctctgtc cgacagaaag
     2401 ttcatcggga taccgtttga gcagttcttc tatattcaac aattgagcta catgtcttac
     2461 tctcttttcc atttcctctt tagtcatccg ttcaactgct aggggcaata ggatattgtc
     2521 ttttgcattt agggtatcga tcaaattgaa ctcttgaaaa atgaatccta gtttttttcg
     2581 acgaaagtca ctgagctcac tgtttttcat ttttgtgatt tcttttcctt ggatcgagac
     2641 actgcccatc gtaggcaaat tgatcgtgga caagatattc atcagcgttg tctttcctgc
     2701 accacttggg cccataatcc caacaaactc gccttcttct actgtgaatg agatattatc
     2761 taaggctttg acgggagaaa gtttgctgcc gtagctttta cttagatgtt tcacttcgat
     2821 catcttttcc aattcttctc ctcctctttc atttttcatt tctatttatt tgcatagtag
     2881 catatctacg aataatttgc tcttatgttt ttgtcacttt tacttggact gaataaaaaa
     2941 ttttattcaa ttattttatt cagcaaaatt aaatatgcaa aaaacaaatc caccagctac
     3001 aagttttcac cttttttcac aataaaatat acactacata tagtatagaa atccaattat
     3061 acagcacatt gaatactata tatagccgat atttagttgc tccttccccg attttgtttt
     3121 atgatagaca agaacaattt agcaggagtt gaagataatg aaagtaaatt atgaagaaaa
     3181 aactgtcgtt actcagcagc cagagacatc ccagatgaaa gtcatcaaac gcgatggccg
     3241 ccatgttgat ttcgatgaac aaaagatcaa tgaagcactg atcaaagcag aaaagaaaat
     3301 tcacggctca ttatcaccac ttacttatga aaaaattcaa atgattgcag acttggttgt
     3361 ccaagaaatc aaaagccggt ttgctgaaaa tgtcaaaatc tatgagattc aaaacatcgt
     3421 ggaacatata ttactagaaa aaaacgaata tgaattagca gaagagtaca tcaattatcg
     3481 taccaaacga gattttgctc gcagtaaagc aacagatatc aacttttcga ttgaaaagct
     3541 aatcaacaag gaccaaactg tcgtgaacga aaacgcaaat aaagacagta atgttttcaa
     3601 tacacagcga gatctgacag ccgggatcgt tggaaaatca atcggactga aaatgttgcc
     3661 accacatata gcaaatgcgc atcaaaaagg cgatatccat taccatgatt tggattatca
     3721 tccttataca ccgatgacga attgctgctt gattgatttc aaaggcatgc tgaacaatgg
     3781 gttcaaaatc ggcaatgccg aagtcgaatc gccaaaatct atccagaccg ccacagcaca
     3841 aatctcacag attattgcca atgtagcgtc aagtcaatat ggtggctgtt ctgccgaccg
     3901 aacagatgaa ttgcttgctc cttttgcaga attgaactat aaaaaacatc taaaagacgc
     3961 ggaagaatgg atcgattcgc cagaacgcca aaaagaatat gcaaaagcaa aaaccaaaaa
     4021 agatatcttc gatgctatgc aaagtttgga atatgaaatc aataccttat ttacttcaaa
     4081 tggacaaact cctttcactt cattaggatt tggactagga gaaaactggt ttgaaagaga
     4141 aattcaaaaa gcaattttac agattcgaat caatggatta gggagtgaaa agcggacagc
     4201 tatcttccct aaactgatct tcactttgaa aaaaggtgtg aacttaaacc ctgaagatcc
     4261 taattacgac atcaaacaac tagcattgga atgtgcaacg aaaagaatgt atcctgatat
     4321 tctgaattac gataaaatcg ttgaattaac tggaagtttc aaagtaccta tgggctgtcg
     4381 ttcattcttg caagggtgga aagacgaaaa tggtcaagaa gtaaatgtcg gacgaatgaa
     4441 tttaggtgtt gttacattga atttgccaag aatcgcgatc gaatcaaaag gagatcagaa
     4501 taaattctgg cagttactgt ctgaccgctt agaaatcatg aaagatgcgc ttctctatcg
     4561 tgtagaacgt tgtaaagaag ccattccagc aaatgcgcca attttatata tgtatggtgc
     4621 tttcgggaaa cgtctttcac gtaccgattc tgtcaatgaa ttattcaaaa accgtcgagc
     4681 tactgtatcg ttaggctaca ttggattgta tgaagttgct tcagctttct tcggtggcga
     4741 atgggaaaca aacccagaag caaaagcttt cacacttgat atcgtcaaag aattaaaagc
     4801 taatgcagat gcatgggggg atgaatatgg ttatcatttc agtgtctatt caactccaag
     4861 tgaaagtcta actgaccgtt tctgccgttt ggatacagaa aaattcggca tcatcgaaaa
     4921 tatcacagat aaagaatatt atacaaacag tttccactat gatgtgcgta aaaacccaac
     4981 gccatttgaa aaattggaat ttgagaagga ttatccaaaa tattgttcag gtggattcat
     5041 ccactactgt gaatacccta tgctccaaca aaatccgaaa gctttagaag ctgtatggga
     5101 ttttgcatat gacaaaatcg ggtatcttgg tacgaatacg ccaatcgatc attgctatca
     5161 atgtggtttc gaaggtgatt tcaaaccgac agaacgaggg ttcgaatgtc cagaatgtgg
     5221 aaatcatgat ccaaaaacat gtgatgtagt caaacgtact tgtggctatt taggaaatcc
     5281 gcaagctcgt ccgatggtac atggccgcca taaagaaatt tcctcacgtg tgaaacacat
     5341 gaaatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agaatagcta aacaaaaata
     5401 gttcctcata tttttcgttt agttgggctt attactgcag atgcaagctt ttgaacaccg
     5461 tttattcaga ataagaggct atgacaagtc ttttttcacc accctctttc gatggctttg
     5521 tatctttgaa cgccctctat caaaataaga acaagctttc ttgaacaatg gatttaggtc
     5581 agtttgttct tatttatgtg gtcgagtaga ttttatcttc aatagctata tctttgaacc
     5641 ttaaatgaaa gaagtttttt tatgagaaat ccaaaaccaa aagaatggtt agcaaaagac
     5701 tacagccaaa actatattgc tgactacaaa ccgtttaatt ttgtcgatgg tgaaggtgtc
     5761 cgcaacagtc tttacgttag tggctgctta tttgcctgcg aaggctgctt caacaaagct
     5821 gcacaaaatt ttcgatatgg caaaccattc acacctgaat tagaagaaca aatcatcgct
     5881 gacttgcgca acgattacgt gcaaggatta actttacttg gtggcgaacc ttttttgaat
     5941 acagatgttt gtctgcaagt cgtacgacgt atccgaaaag agttcggtac gacaaaagat
     6001 atctggtcat ggacaggcta tacctttgat gaattgctac aagattcaga agataaatta
     6061 gaactccttt cgcaaatcga tatccttgtt gacggacgct ttgaattaag caaacgtgac
     6121 ctaaaactac aatttcgcgg aagtagcaac cagcggatca ttgacgtcca aaaatcttta
     6181 gaatccaatc aagttgtgat ttgggaaaaa tgtacggatg caacagaaac ctatgaacaa
     6241 atcaaaaagc aagacttgat ataaaaacag ctttgggaga gaaaacggat ttccggtttc
     6301 aactctcaaa gctgttttta tatttcttga ttttcccata atggaaggac gatgttttca
     6361 taagtcatcg ggtccagatt cgtcaaagta atccctaaca atcggattcc tttttctact
     6421 cccaagattt cctcccatat aaggcttgct tgataaaaga gtgcttcttt tttatagata
     6481 tattctggca atgtcactct tttggtcaca gtagaatagt ccgtgtacct gaccttcaaa
     6541 actactgttt tcccatgttt ttggactctt ctcagtgctt cttctacttt ttcagctagt
     6601 tggcgcaact gagtcagcac tgcttcttcc gtttgcaacg gctgcccata agtatgctct
     6661 tttcccacag attttcgctc tcttgtaacg tttactggag aatcatggat tcctctgact
     6721 ttacgataaa gagaatagcc catttttcca aaatttcgaa tcaacatcat ttctgtgcat
     6781 tcatataggt cttttcctgt ataaatccct agctcatgca ttctaggtac agttttcttc
     6841 cctaccccat gaaatttttc aattggcaaa gcttttaaaa atggcacagc gtcttgaggg
     6901 gcaactaccg tgattcctct aggtttttga tagtctgatg ctaatttggc caaaaactta
     6961 ttgtaactta ctcccgcaga acatgttagc tggacactct cccaaatatc tgcttggatc
     7021 attcgggcga ttttgatggc tgacttgcaa ttgattttat tttcggtcac atcaagatac
     7081 gcttcatcga tactaactgg ttccacaata tctgtataac gatagaatac ttcccgtact
     7141 tgtttggaaa tatcgctgta tttatgatag tcgccaggct taaagatggc ttgcggacaa
     7201 agctcatacg ctttttgtgc actcattgct gaatgaattc cgaattttct tgcttcataa
     7261 tttgctgtag tcactactcc acgcccacca gtgtcactgg ggtgtctggc aattactaat
     7321 ggatgtccgc gaaactcagg atgatctcgt tcttcaaccg aagcgaaaaa agcatccata
     7381 tcaatatgaa tgatttttct tgatgtatca ttatgtaaag gaaattgcag ctccatcatc
     7441 ctcacctctt attcatcata caagaacatt cgttcgcgtt caagagattt gaaaagttag
     7501 tagctgattt cctcattagt ctttcattat agacaactta tactatctca gtctaattca
     7561 tggtactgac ggtagacttc ttgttttttc agcccatttt tcagcgctac tgctttgata
     7621 gcagcatttg gtttttcgcc tgaagcgatc tttgcttcta cttgttcctt taacgttccc
     7681 aactcttcat caacttccgt cactggcatt tcgaacaaat tcccttctac aagcagacaa
     7741 cattcaccct ttactggatg ttcttcgata taagcgagta gctcttccgt acttcctcta
     7801 aggtattctt catggacctt tgtcaattcc cgacacaaaa cgacttggcg atttccaaaa
     7861 atttctaaga aattcgtcaa tgtagcagtg atccgataag gtgactcata aaaaatcagc
     7921 gtaactgtct gttcttttag ctttttcaat gtttctttct gttctttttt cttacgtgga
     7981 aggaagccat aaaataaaaa tggttgaggc actagccctg aagcaatcaa agccgtcatc
     8041 ccagctgtag gtcctgggat agagatcaca gaaatccctt cttttatgca ggctaaaact
     8101 agctcatgtc caggatcgct gatggaaggc attcctgcgt cactgacttg agcaatcgtt
     8161 tttccttctt ttaacaaagc aatcaattga gggacacgtt ctttataatt atgttcatgt
     8221 aaactttttt ggggtgtatg gatttcaaaa tgattcaata acttttgtgt attacgcgta
     8281 tcctcacttg cgataagatc ggattcttgc agcatacgaa cacttctgta gctcatatct
     8341 tctaaatttc caattggcgt cggtacaaga tataaatccc catataccgg atttcccttg
     8401 aaactttttt gattatacat ctgtcacaat ctccttaata aaaagactgg agcagtattt
     8461 ctccgctcca gtctcctctt gttattggtc atcgaccgct ttcgcgatag atcacttcta
     8521 gacaaaacgc acaaggctcg tcattgtccc ttcttgatcc atataaatcg tagcagacat
     8581 ggaatccttc ttcataaatt ttttcaagat tcatacgaga cttagaaagt tcctgcttca
     8641 cattttcagc tggagcttgt gcaacttggt tcaattcccg caggtgttcc cgcagacgtt
     8701 gattttccat ctccaaagtt gtattttttt ctacgatctc atgcaaagca tgcttcattt
     8761 caacaagttc tgttaatgta tttctcaact ctgattctaa ttgattcaat ccgtcgtata
     8821 atgatctctt atccattcat ctcacctact tggataaatt ctttcaattc atcccattca
     8881 tattcgacag gattttcccg tccagtcagc cgaatactaa cgatacggct aagcagattc
     8941 aaccctacga ctttcccttt accatcaggt gtagcaatta cttttccaaa gtcagggagt
     9001 tcttttttcg ctgtctcgta ttcttcgctc tcatattgca gacagcacat caagcgtccg
     9061 cataaaccgg aaatttttgt tggattcaaa gataaacttt gatcttttgc cattttgatt
     9121 gatacaggca cgaaatcgcc taaaaaagaa gaacagcata atggacgccc acatggacca
     9181 attccgccta ttatctttgc ttcatcgcgg acaccaattt gtcgaagctc aatacgagta
     9241 cgaaatacag cggccagatc ttttaccaat tctctaaagt caattcgccc gtccgctgtg
     9301 aaagaaaaaa tcaatttatt tcgatcaaat gtgtattcgg ctttgaccat tttcatttcc
     9361 aactcacgca actgcacttt ttcttttgcg attttcaatg catcataagc atcttttttg
     9421 ttttttttgt ctttttctaa ttcagcttgt gacgctttat gcaagatcgg acggatagct
     9481 tcaggaaggt cattgggatc aatctctttt ttaggaagga caacagtagc aatatgtaag
     9541 gattgctgag attcgactaa taccttgtca ctataaacat attcagagtc tcctggtgca
     9601 aaataataga tatgacctgc atctctatat cggacaccta ctacttctac catttccatc
     9661 ctccttgatc atcatccttt gcggcaagct aaaaaatcgt atgcaaaacg aactgttccg
     9721 caacagcctg aaaactgaca ttgctgcgta gtttctgctc tgcgatcaat atccgttcaa
     9781 ttatctggtt tatctgtttc actttgccat attcgccgtt tgctactgct tgacgctgtg
     9841 cttcctgata ataaaacatc aagacagata atcctgtaaa ctgttgtgtt ttttctttaa
     9901 atgattttac caattttttt tggacataga taaaggcttg agtatcttgc ttttgcaagt
     9961 aaacgaacca ttgataaatc aagtccttag catcattaaa ccattcatct tgagagattt
    10021 caactgcttt tcctaaactg tttgttaaga aagccaaaag ttttgctttt tccaatggaa
    10081 tctgctcttc ttgcaatcga tggatcagtg cttctttcga aagttcttga aaatgcagaa
    10141 cttgacaacg agattgaata gtagggagaa ttcttcctac tgcatctgtt tctaacaccg
    10201 ctaaaaaatc tccagggggc tcttccaaga atttcaacaa actgtttgcc gcactgctat
    10261 tcattttttc agcttctttg atcaaaaaaa ctttttttct tgattcgtaa ccgctgcgac
    10321 tgaattccgt ctgcaaacgt ctgatctgat cgactttgat ggtttgtcct tccggttcaa
    10381 taaccaaaac atctggatgt tctttctttt cgatccgctg acaattgttg cattttccac
    10441 aaggcagatt gtctttcatt tgggtacaga acaggtgctg tgcaagccat atacctactt
    10501 catgttttcc tgttcctttc tccccttcaa aaagataagc atgagcgata cgcccatgct
    10561 caaaactccg ttgaagctgc tgatacacga tcggttgcat ttgagccaag tttatttctt
    10621 gattcatagc tcagctcctc ctaaaattga tggaatcctt caacaggtaa gacgaagact
    10681 gtggcaccgc ctacttctac ttctactgga taagggacgc ccccatccat cgaaatatcc
    10741 agtgtaacag gtgtagatac aaattgcttt cttgattcac acgttttttt gatgatttcc
    10801 aatgcttctt ctacacggtc atcttcaata cccacgataa acgtactgtt tccagcctta
    10861 aggaagccgc ctgtcgatga tagttttgtg gcacggatat ttgcatcaat caattcattt
    10921 gataaacggt tgctgtcttt gtcttggata atcgctaaaa taattttcat aaacgttcac
    10981 cttcctagtt ttcaaaaaat tgcggatact gttcaataat tgtatgataa cttgtttgca
    11041 gcacttcttc aaagcttttt cttgcatcaa ctttgtggat tctttccgga ttctcttctg
    11101 ccagctttaa gtaagcatga cgtacacgct ggtggaattc caaaccttcc gaatctaaac
    11161 ggtcgatttg attttgtctg tttttcttga ttcttcttaa tccggtgtct gaatctacgt
    11221 caagataaag ggtaaaatcc ggcgtagtac cttctgtagc aaactcattc aaccgagcaa
    11281 tctcggtaac gccgatgcgt cggcctgcac cttgataagc aagagaactg tcgacaaatc
    11341 tatcgcataa aacgattttc ccttttgcca gagcaggcag aaccttttcg actagatgtt
    11401 gtcttcttgc tgctgcataa agcagtgctt ccgtacgttc atccatccga tcattttttg
    11461 gatcaaggat cactgctcgg atctcttcag caatcggaat gcctcctggt tctcttgttt
    11521 gaatgatttc tgtttttgct acttctttca aaagagggta aagttcattc aagatgctgg
    11581 tttttcctgc accatcaggc ccctcgattg taataaatag tccgttcaca ttgttgcccc
    11641 catctcttgt gctgctttta tattgtatct gaaaaaacac ttaattagaa atttataact
    11701 cacgtcttcc ttcgacagct ttcaataaag taacttcatc tgcatactcg atatcagagc
    11761 ctacagacag accatgtgct agacgagtca ctttgatacc tgcgggtttg atcagacgtg
    11821 agagatacat agctgttgct tctccctctg ttgttgcatt tgttgcgata atcacttctt
    11881 ttacatctgc atcatgcagc cgctgcaaca gagaagaaat attgatgtct tctggacctg
    11941 tcccttccat cggtgataac acaccatgga gaacatgata aagtccgtga tattctcgca
    12001 ttttttccat tgccattaca tctttcggct cttcgaccac taggataacg cttcgatcac
    12061 gtgaaggatt tctacatatt tcgcagggat cttcttctgt gatgtttccg cagatactac
    12121 aaaaatgcaa atcgcgtttt acacttaaca aagatttagc aaattcattt actacttcat
    12181 ctttcatatc gatcgtataa aaggcgagtc ttgtcgctgt tttctgaccg attcctggaa
    12241 gtttcatgta gctgtcgatc agtttcgcaa ttggttctgg atattgcata ttcattactc
    12301 cttcagttta aaactaaaat cctggcaaac cttttgcgta tttccctaat tttttctcag
    12361 tttccacatc aatttgtgcg atcgcatcat tgactgccat tacgacaaga tcctgaagca
    12421 tctcgatatc atcaggatca acgacttctt cattgatttg gatatctttc atgcgacgat
    12481 cgcctgtaaa aaccgctttg accaattcat tggtggcttt acctgtaaac tctgttttat
    12541 ttagcagctc ttgtgcttct gccatttctt tttgcatttt ttgtacttgt ttcatcatgc
    12601 cttgcatatt gcccattcca cgcatcataa ttatctactc ctttttatta atcatctacc
    12661 actgtaacag cttcggagcc aaacagttcc tctgctttta ctacaagtgt ttcttctggt
    12721 acttcttgtt cttgaggaag caactcgatt tctccttctg agtcttcaga agcaaaagag
    12781 ttttctgttc cctcttcttg tgcctgcgtc aaataatttc ttcgaagttc tggccagctt
    12841 tctcttgtaa tataaacagg atcaggcgca taatctttga tcattcggct taagttgttg
    12901 tgtaccgaaa gctgcaattc ttcgtcattt gctgctcttt gacaaacaat ctcataatca
    12961 aacgcaatca ctagtcctgt aggtccagct gcaactggtt cgcttgcttt aagcattgct
    13021 cgctgagtga cagatagact catcagcaag tcttcccata cttcttttac ttggttcaga
    13081 tgctgtcttg tcgcttcccg cagaacttta tatactcgtt ccgtcggtac acgataggta
    13141 cttgtctgtt ttttatgatt tgtttgctgt accggctgta cttttgtatt ctcaattcct
    13201 gtattactgc gtagttcctt gatttcgttt cttagttcct taatctcttg ttgtagctgt
    13261 tttactatct gatcatctgc aaatgctaca tctgctgaag gaacagaagc agacggtagc
    13321 tgtgcaagtt tgactgtagc gacttccaaa taaatagcgg cactattggt aaaacgaatt
    13381 tcattttgag tatcgctcaa aatctttatc attgaaaaca gctgatccgc tgaaacagtt
    13441 tgtgccaatt ctgtaaatgt acttctgatc gtactcgatt gttcctcaac taggttaggt
    13501 gcttgctgat acatcagcaa gtcacggcag tattgtaaca tatcctctag aaagcgacgt
    13561 gattctttgc cggctgcgag aatttggttc aatgtttcca atgcttctgg tacttctttt
    13621 ttcacgcaag cactcaagta actgtccatc atttcgtcag ttaagctacc tgtcacttgc
    13681 atggcatcat ctaaagtaat tgttccttca ctaaacgaga ttgcctgatc aagaatactc
    13741 aatgcatccc gcatcccgcc ttctgcagaa cgggcaatca cattcaatgc agctggttca
    13801 tagctgatag tcgattcttt caagataaat tcaagatgtt cgacaatatc tgccgtattg
    13861 atccgtttaa aatcaaatcg ctgagtccgt gaaatgatcg tagctggtat tttgtgaggt
    13921 tcggtcgttg ctaagatgaa tatgacactt tcttttggct cttccaatgt tttcaacaag
    13981 gcattgaaag ctcctgttga aagcatatgt acctcatcaa taatgtacac tttgtacgct
    14041 gcctttgtag gcgcataatt tgcccgatca cggataaatc ggatttcttc gactccgtta
    14101 ttgctggcag catcgatttc gatcacatct tcttgcgtac cagcagtaat tgatcgacac
    14161 atttcgcatt cgttgcatgg ctcaccgtct ttactgttcg gacagttgat tgcttttgcg
    14221 aaaatctttg ctgcactcgt ctttccggtt cctctgggtc ctgtaaacaa gtaggcatgt
    14281 gaggttttgt gggaaacaat agcatttttt agtgtctgtg tcacagcttt ttgtccaacg
    14341 atgtcttcga aacgctgcga acgccagact cgataaagtg cttgatatgc cataaggatc
    14401 cccctttttt aaaaaattca ctatctctta ttatacgtga tattaggtaa aaaaactact
    14461 atccttctcg gctttttcag gtatttaaac aatacgcaat tttccatttc aattccgtct
    14521 ctagtccgag aagtttcaca tcttcctatg gtataataaa taaacaataa ttgtttggca
    14581 ctagctgaat cagaggagga attttttatg ggtcttatca aagcagcaac aagtatggtt
    14641 ggcggcggtc tggcagatca atggctagaa gtcatcgaac cagacaatat gagtgataca
    14701 acggtcatga caaaaggtgt caaagtccgc aaagatgaca aacgtggctc caatcgtaaa
    14761 ggaacagaag atgttttaac agacggaaca gtcgtccatg tctatccaaa catgatgatg
    14821 cttttggtcg atggtggaaa aatcatcgat tataccgctg aggaaggtta ttacaccatt
    14881 aaaaatgacg cagctccttc catgttcaat ggaacactga aagaagcaat tgctgaaact
    14941 tttgaccgat tcaaatttgg cggcgtcaca ccgcagaaac aacaagtttt ttatatcaat
    15001 ctccaagaaa taaagggaat caaattcggt acaagcgcgc cgttgaacta ctttgacaat
    15061 ttctataacg cagaattatt cttacgggca catggcactt attctattca tgtcgttgac
    15121 ccgattttat tttatacaaa cgcaatccct aaaaacaaaa cacaagtgga aatcaatgat
    15181 atcaatgagc agtatttagc tgaattccta acagcgctac aatcagcaat caatcagatg
    15241 tctgcagatg gtcaacggat ttcttatgta ccatctaaga gtttggaatt aagcaaatac
    15301 atggatactg cattggatga ttcttggcgt gaattacgtg ggatggagat tgtttctgtg
    15361 gctgtagcca gcatttctta caccgatgat tctgtgaaac tgatcaatat gcgtaacgaa
    15421 ggggcgatgc taggtgatcc tagcgtacgt gaaggctacg tgcaaggttc cattgcacgt
    15481 ggtatggaag cagccggaaa aaatgaagca ggagcaatga ccggctttat gggtgtaggt
    15541 atgggaatga atgcaaatgg ttcttatctt tctcaagcag ctcaaaataa ccaagaacaa
    15601 atcaaacaac aagcagaaaa acaaaatcaa caaactgcac aaggaaatgc ggatacttgg
    15661 acatgtccag tttgcgggac agaaaattcc ggaaaattct gttccaactg cggagctgcc
    15721 aaacctgtag aaaacgttca gcctaagcta caaatgcgat gcagtgaatg caatgagatc
    15781 gtggatcttt ccaacggcat ccctaaattc tgtccaaact gtggtaaacc gtttaaagga
    15841 atccccgtta attaatttat ttagaaaggt gtgcagctga tggatgttct tacacataaa
    15901 tgtcctaatt gcggcggtcc gttgactttt gatcccaaag accaaaaatt tcattgcgaa
    15961 tactgcctga atatctatac agaagaagaa gtcagccaat atgagcaaaa gcaaaaagaa
    16021 gcgcgtatgg ctggcgaaga aagcaaagaa gaaccttcac ctaccgaaga ttttactttt
    16081 acagcccaag aacagcttgc cgatatgaat gaagccgagc gcaaagcggt tgacgaggca
    16141 ggcggttttt cagatacagc ggacgaatct gcagataata ccgcagcgat agatcttttt
    16201 ctttgtccca actgtggtgc agagatcgtg actgatgcga caactgctgc gacttattgt
    16261 tactactgcc ataatcccgt cgtactatcg ggacgtttaa gcggagaatt cttgccaaat
    16321 aaagtacttc cttttgctgt cgagaaagaa gaagcaatca acagatttct cgcttggacc
    16381 aagaaaaaat ggtttgttcc caaagctttt ttcaataagg atcaaatcga taagttgaca
    16441 ggtgtgtatt tcccttattg ggctacagat gctgaagtag atggtgccat gcaggcgaat
    16501 ggtacagtta tccgcatatg gcggataggt gacatcgaat acacagaaac gaagcaattt
    16561 gctgtttcaa gagcaggcaa gctttctttt aaagaactag taaaaaatgc cttatcaaaa
    16621 aatacgcaac aaaagatggt cgaaggcgtg caacctttcc cgttagataa agcaattgat
    16681 tttaaaagcc agtatttagc cgggttccaa gcagaaaaaa gagatatcga atatgaggca
    16741 atcaaatcac agattcaatc tgaattaaaa gactattcag agaagctgtt aaaagatacc
    16801 gcaaatggat atacaacatt gaccggtgtg cgaacttctg cttcaatcac aaacgaagta
    16861 aataattacg tattactgcc ggtatggctt gttacttatc gaagcaatga ctcaagtaaa
    16921 aaagtctact actacgcaat gaatggacaa acaggaaagg tcagcggtgt cctgccgatc
    16981 agccagaaaa aattgggact gaccgctctt gggattttcg ctgggctagc gatcctcttt
    17041 ctgatcggag gttacttgat atgatgaaaa aatttttttt attctttctt ttgctagtaa
    17101 gtgttggctg gccggtggct actcatgcag atacagctac ggtaaatgat gaggctgggt
    17161 tattcacaac agatcaaatc caatctttgg aacaacaggc agaaaagctg aatgaaaaaa
    17221 tcaaaggacg agtcttgatc gtaacgacta catctaattc agaagaacca agagactttg
    17281 ctgataatta cttaagagac gcagtcggaa atgatcaaaa tggctcagta ttgctattag
    17341 atatgggaca gcgtgaaatc tacatttcga cttctggtaa tatgatcgat tatttgactg
    17401 atagcagaat cgattcgatc ttagatgatg tttatgatca gatgacaaat gctgcctatt
    17461 acaatgctgc aaaagcttac ctgtctaaat cagcaacata tgtggaagac ggagttccag
    17521 gaggacatta tcgtgtagac gaagaaactg gcaaaattac acgctataag gttttgacga
    17581 cagtagaaat catcattgct gttgttttgg ctcttatcct tagtcttgtc ttttattttg
    17641 tgactgtttc gcgttatcaa ttgaaatcag gcatctacaa atatccattc cgagaaaaat
    17701 ccagtatcaa attaactgaa aaaaccgatc gtctgacaaa ttcttttgtc acgacaagaa
    17761 gaatccccaa aagcccacct cctggaagcg gcggtggcgg aagtactacc cattctagcg
    17821 gtggcgggac ttttggcggt ggcggcagaa gtttctaaaa attgctaaat gaatgttcgc
    17881 aaaaaaaggg ctgtgacaaa aatcgtgtca cagccccttt tgttgcaaat aaacggcgtt
    17941 caaaagctta catctacggt gataagtcca gccaagttaa aaacataaga gactgttttt
    18001 acttggctct tcttattact tgatgcagaa cagctttttc acaacctctt ggtaaacggt
    18061 gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga aaagctgttt
    18121 tttgaaattg ttccttgtga ctcagagtta aacggcaggc tcacaacctc ttggtaaacg
    18181 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    18241 tttccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttgttcat
    18301 caaaagataa tattgtcttc taacaagtcc atattcaaaa taacgatatg atggtcttta
    18361 acggtgataa taccatcttt tttcagttgt tttaaaattc tgtttacact gctagcagtt
    18421 gagatgccac aaaatttggc gatttcttca ttcgtgatcg aaaaatcgat cagcatccct
    18481 tcttctgtcc gcacaccgaa taaatcatat aattcgtaaa gttgcgtaca aacagctccc
    18541 agctttccgt ttaatagcat ttgctgcatt tttttcatcg aataaagcag acgatagcgg
    18601 taatactctt ttacatagtg ttgtagcttt gggtcttggt tgatattttg ccaaaagcgc
    18661 acacgattga ttcgataaaa ctctgcatat tctgattcaa ctcgaatatt atacggcgaa
    18721 tcgatgaatt gtgaatattc gtcttttaaa agagagacga tttcaagatc tttgatatag
    18781 cgaaggttaa attctctccc atcatttgaa ataacacttg cttttaccgt tccctctttc
    18841 aagatatacg tatgggaatc ttccatccct tcgaatgtca aatatttatg atatcctttg
    18901 cgtataatcg gaaaatcatt tttatccaaa tattccttta agactctact atccaatgat
    18961 gctcccccat ccaaactttc atctaaaaag atcatacagt actttaaaac tttttaaagt
    19021 ccaaaggtat tttactaatg atattaacat ttgttaagta cctttttcca tcacttatgc
    19081 gatttttttc ctttttttta ttcgttatac tgcagaagta aaatttttta agaatagagg
    19141 gaaaaaacat gaattactgg atgaacacaa tcattaatcg attagaaaca gcatatcaaa
    19201 caagatttga tatgaaagct tctttagtct ttttaaatga tgcgtatcaa aactcgatcg
    19261 aattgatcaa agcagtggat gagaatccaa caaacgaatg cgaagaattt ttaaatctgt
    19321 ttatgtctac aagagattta tttattcgcc aacttgttga tcgctatcca tccaattatc
    19381 acgatgtaga agttcaaata caaaaattaa aagcatattc agcttaaggg gaatgtataa
    19441 catacatgga aaagaaatta tttaataaat attttattgg aatcacattg atcaatttta
    19501 ttgtctacct gatttattat ctgttgatgg tcatcatggc agtcattgcg caagacacct
    19561 tacacgccag cttgagccaa gcaggttttg cttctgggat ctatatcatc ggaacattgc
    19621 tggcacgctt attcatgggg aaacagttag aactatttgg tcggaagttt accttaagag
    19681 gcggcgcgat cttctatctc ctttcaacat tagcatatct ttatacgcca actatcgggg
    19741 cattgtattt agttcgattc ttgaatggat tcggctacgg tacagtttct acagctacaa
    19801 atgctatcgt tactgcatat atccctgcat ctaaaaaagg ggaaggcatc aattattatg
    19861 gattaagtac tagtttagct gccgctgttg ggccattttt aggaatgatt ttgctgaata
    19921 tgactgattt tcgtttcatt atctggtttt caattgtact ggtcttcttt gttacgattg
    19981 cctgccttgt ctttcctgta aaaaatattc cattatctga agaacaaaaa atatctcttc
    20041 gctcatggaa tatcaatagt tttattgaaa agaaagcact tttcattaca ggaattgcct
    20101 ttttgatggg tctttcttat tcaagtgttt tatcattttt atcttcttat acaaaagtaa
    20161 tccacttagt tgctgctggg tcatttttct tcgtcgtata tgctttggtc atcacattta
    20221 cacgtccatt gactggaagg attttcgatg tcaaaggaga acaatacgtg atgtatccta
    20281 gctatttatt cttaacagca ggattatttt tactgagtgt aacgacgaat agtgtgacat
    20341 tgcttatctc tggagcatta gtcggcctag gttatggtac gtttatgtca aatggacagg
    20401 ctgtttgttt aaaaatcgtc cgcgaacatc gaatcagtat tgctttatct acctacttta
    20461 ttggtttaga cctaggctta ggcgttggtc cttatttgat ggggatgcta aaatctctta
    20521 cttcatttag aggattgtat gtgattgccg gagtcattcc attgatatgt acagcactct
    20581 atcttgttaa tggccgaaaa catagtcatg aagcagaggg cgaattgatt gaatcattga
    20641 aaaatgaaat ttgatcttga tgaatagata aaaaaaaccg ttcagatctg aaaataatct
    20701 gagcggtttt ttgagtaaaa atagtttatg attattctta attaaacatc tatttatttt
    20761 taaagctttc atttatcgat aagaatagtt taactgttga tttattccat tttccgttca
    20821 tctggtacga tgccttgact tagcaaccac ttgtggaaga accattctgc cacaccgatc
    20881 aatagagcag gaatcaatac tttcatccaa atatcgactg tatcattcat aaaatataga
    20941 tagagccaca agataactgc agctgaacca gcatctgcga tcgtcgctgc gatgttccct
    21001 actttacgta agatcaacag atcccctagc acatagatca tgactacaat aattacccca
    21061 acaatcgttg aatccataaa gctaacaccg tataaacctg tcagcacgac ccacagaatc
    21121 gcccagatca tgattgcctt cactaccaac gccttcacat gtttcatttt ctgcactcct
    21181 ttctcctgga taaaaaagta agattatcta acaagataac cttttcatta ttataccatc
    21241 ggctgagaga agcgagcaaa gataaggtcc tatctaaaaa tgaggaagcc acttagaaca
    21301 ttcacgtaat aatttatccg tacagacatt cgatatggtg ttcctttact ttcgcatact
    21361 gttcttttgt cagttcttta cttgtcacca gataatcgat ttcatgaaaa gcaatggctt
    21421 gatgcatgaa tttatgtgca attttttgct gtgtaactaa aacggcaagc cggtctgatt
    21481 gttttctaaa agcttttttg accattgcct ctccttcatg agtttctgtt actccttggc
    21541 tgaaatctag cccgcgacaa ctcattatcg ctaaatccgc ataatatgac aatgcatcgt
    21601 tataggcttt tgaaccgttg attgttgctc gtttcggaac aattgcccct cccaatacag
    21661 atacttttgc cgttgtatat tcactcaaca tagcagctgt gacgatgcca ttcgtaatga
    21721 tctgcaaacc atcaaatctt gctaggtttc tgactaagtg taaagaggtt gtactggaat
    21781 ccaagaataa gaccatatca tctcgaatca aagaagaagc tgtatctgca atcttacgtt
    21841 tttctgtttc ttcttctaat ttggtgatct tcatcaggta aggcacgtca atttttcgat
    21901 tgtcaataag catggcgcca ccgcgaactc tacggatcaa tccacttttt tccagaaaaa
    21961 caaggtcacg tttaatcgtg gcttcgctat aatttactaa ctcgatcaaa tcggtgactc
    22021 ttgcaaattg gttgtttttt agataattca caatctcctg ctgtctattc tccacttcat
    22081 tcaacataaa atcaccccat ttactttata tagtaacagg aaaaaagagg ttgtgacaga
    22141 agtgattggc ttcgagaaat aagaagaaat ttttcgaaaa aactacttct gaacatcgtg
    22201 tgtggttaag agggtatgac aagactattg tcataccctc ttgaccacac atttttattt
    22261 ttagtataaa ctagccttgc ctactgtgtt caaaatttcc attttatgtt tgaccacttc
    22321 ttgtaccttt ttattagctg ctggataaat tgcattcggc tcgtacatcg atggattttc
    22381 actcaatatt tctctcaaag cattgaaaaa gacgctttta agatctgagc tcaaattcac
    22441 tttggcaact ccgtatttta ctgcttcgct gacttctgcg tccggattac ctgaaccgcc
    22501 atgtaaaaca aatggaagat cgatttgttg gttcaattct ttcagtaagt cgatatttaa
    22561 ttttggagtt ctgtttttcg gatacaagcc atgcgctgtt ccaattgcta cagcaagtgt
    22621 atcgattcct gtttctttca caaaaatctt cgcttgatca ggatctgtat aaatgattgt
    22681 ttcagcacct ccttcagcag aaccattatt cccaatcgtt ccaagctcag cctccactga
    22741 gacatttacc gcatgagcta acgcaaccac ttttttcgta atctgtacat tgtcgtcgaa
    22801 tgatttcaaa gacgcatcga tcatcacgga agaatagccg tttcgaatcg cccgtaagac
    22861 atcttctatc gtaccgccat gatccaagtg gataacgatc ggcagtttac ttgtataagc
    22921 ataggctctt actgccgcaa caaactcatc acttaaatag gcgatttcat caggatgaat
    22981 ttctaagatt actggtgctt ctaatctctc tgcttcttcg atgattgctt tcatcatatc
    23041 atagctgcaa atattgaagg ctggtactgc aaattgattc tctttagcca ctttcaataa
    23101 ctctttcatt gtatataaca ttacgcttcc acctcatttg ttagattttc atttttcgtc
    23161 cacttgtctt gttttttctc tttcactgtt tttttcaata gtccaagcat aaaggctgta
    23221 gtgactgtac ctgatgcaat tgccaagaca tacataggta aattgttcat aacatttgga
    23281 attagcatca cccaaatccc tccatgaggt gccattgaag taactcctac tgccatggaa
    23341 atccctgcag cgactgctga acctgtcatc aaactaggta tcacgcggat cgggtcagat
    23401 accgcaaacg gtatagcacc ttctgtaata tacgaagcgc ctaaaatcca gcatgattga
    23461 cctgaagtcc gttcttcttt ggtaaattta tgtttgaata ataaagtggc taaagcaata
    23521 cctaggggag gtgtcattcc tgcagccata caagcagcaa tcggtgcatt tactccagct
    23581 gacatcaaac cgatcgagaa agtcgaagtc gatttattga tcggcccacc catatcagct
    23641 gccatcatga tcccgataag taatccgaac aatagcccgg atgtatttcc caagctattc
    23701 agccaatttg tcaaagcatc caacacccat gtgatcggtt gttcaatcac aaataccatc
    23761 aaaagcccaa taattaagat cgataaaaag gggacgacaa tcagctgata aatagcagaa
    23821 aattcttttt ttacgctaat tttatttgcc agaaaatcac agatataccc tgctagcacg
    23881 ccaccaatca ttccgccaag aaaacctgaa ccacctgtag aggccagtaa tccggcaaca
    23941 attcctgggg caaaacctgc ttttccagca atcgatgtag caatccccgc gcctaacgca
    24001 ggaaccatca tggcaaacgc tgtatctcct ccgatccttg aaaatgcagc tgctaacaca
    24061 ttatagtctg ctgagttggg atcagccgct gtaatcccaa aggcaaaact cagtgcgatg
    24121 atgattccgc ctgcgatcac aaatggaagc atgtaattca caccattcat gaaatgatta
    24181 tagattgtcg gctttttctc atttgcagct ggttcttttt cattagacac aacgaatacg
    24241 ccaatatctc tcaatgcttc gtctattact acttgcggtt ctttgattgc tcggcttgta
    24301 gagacacgct ttactttttt cccggcaaat cggtccaatt cgatttcttt atctactgca
    24361 agaatgattg catctgcctc tttgatttct tgttcagata actgattttc tgtttttaca
    24421 ccttgtgttt cgaattttgc ttgatggttt agatttcttg ctgttacttc taatttttct
    24481 gccgacatat aagtatgagc aattccagtc gggcaagcag ttacaccaac gattttcatg
    24541 attattttcc tcctttttct tcactatcga tactcggaca aagactctac tattttcatt
    24601 cttgactaat ctgtctgatg atatctgctg gcgtagtggc ttcttttatt ccctttctaa
    24661 aatctgcatt catcaatcgt ctggagagtg tgctgagaat ctctagatgc atatctgctg
    24721 atttttcagg aactacgatg gcaaaaatat attcaattgg catttcatct atcccgctat
    24781 agtccgttaa tttggctgta tgaaaaaaac acagtgctgg ttttgtcaca tatttgcttt
    24841 ttgcatgggg aatcccaaat ccatcttcta ttcctgtgga gatctcctcc tctcgtttga
    24901 atagtgcctt aacgactttt tgaacatttc ttaccttatt tgtttttttc aacacatccc
    24961 ctattcgctc aaataatgat ccttttgaat ctatcgatgg atcgaaaaca atcagttctt
    25021 ctgcgatcaa ttgatcaaac ctcataaaaa acctctcccc cacaaaaaat gttaacgttt
    25081 tcttggaact gaggtcagta tagcatcgct agctgtaccg ttcaatatta tgaatgaaca
    25141 gaggaaaaag acccatcatc cgcgtgtata agtcattaaa atgaacgatc tagctcacaa
    25201 acaatcgcaa gtctataaca aaacaaaaag ccttccttga aaggaaagct tagcttctaa
    25261 ttttaggtaa atggtttttt acttcgaaat aacccctctt catttcgtta atataaggtg
    25321 tttttatcaa gtggataatt aaaactatat actagttttt cattgttttg tgcattatga
    25381 tcctacgact ttttcagcat caaacgaagt tttaaattgg caaaaaacat aaaaaaacag
    25441 actatcccta tcagatagtc tgctaaacgt tgagttatca gcgtttttat tattccgcag
    25501 cgtttgcgtc tttgagaccg tattttttgt tgaaacggtc cacacgtccg tctgcttgtg
    25561 tgaatttttg acgtccagtg tagaatggat gtgagtcaga agttacttcg acacggatga
    25621 ctgggtatgt attaccgtct tcccattcaa ctgtttcttg tgaagttttt gttgaacctg
    25681 acaagaattt gaaaccagtt gttgaatcca tgaatactac tgggtggtaa tctggatgga
    25741 tgttttcttt catgattttt tcgctccttt gccctgctcc atttgctgaa ccagagttga
    25801 tttgtcgatt tacaacatta acatcttaac atgtttcagt caaggttgca attatctttt
    25861 cagatttctg gtcggatttt ttttaccaaa ggagacatct tgaaattctt tgaaaaattg
    25921 ctgattgttt tttgttttac gcaaaaagcg gatcagttgt tccgtatatt ctaatgaatc
    25981 tccagtcata ttttttcgaa gtttccaaat ttcttctaat tcttcacttg gcatcaataa
    26041 ttcttctttt cgggtacctg attttttgat atcgatggct gggaagatcc gacgttcaga
    26101 taattcacgt gaaagttgta attctagatt tccagttcct ttgaattctt catagatcac
    26161 gtcatccata cggcttcctg tatcgactaa agcggtcgct aaaatcgtca aactgccacc
    26221 ttcttcgatg tttcttgctg ccccgaaaaa tttcttaggt ttatacaatg cggctgggtc
    26281 aatcccgcca ctcaatgtac gtccgcttgg tggaacgact aagttatagg cccgagcaag
    26341 tctagtaata ctatccatca agataacgac atctcgttta tcttcgacta aacgcattgc
    26401 gcgttccaat actaattcag aaactcgagt atggttttgc ggctgcagat caaatgtaga
    26461 agataccacg tcgcctttca cgcttcgttc tagatccgtt acttcttccg gtcgttcatc
    26521 aatcagtaga acgattaatt ctacttccgg atagttttcg gtgataccat ttgcgatttc
    26581 ttttaataca gaagttttcc ctgcttttgg cggtgcgacg atcaatccac gctgaccaaa
    26641 accgattgga gaaaagatat cgatcattcg ggtagatagt ctttgcggtg ttgtttccag
    26701 ttgaagctgt ctttgcggat aaagaggtgt taaggctgga aaatgcggac gttcttttgc
    26761 ttcttccgga tcttttccat taacgctttc cacgtgcatc aaaccataat aacgttccga
    26821 ttctttaggt ggacgggctt ttcctgccac tttgtcccca tttctcaaac tgaatcgtct
    26881 gatttgtgag gcagaaatat agatatcttc tgcacttggt ccataattga ttggccgtaa
    26941 aaaaccgtaa ccatcttgac caacgatatc caatattcct tccatataat aaaatccttg
    27001 tttctctgct tgtgcacgaa tcacagctag agataattcc tttttattca tcttactgta
    27061 ataaggaatt ttaaattctt tggcataact ataaacttct tttaacgttt tgttctctag
    27121 ttctgccatt gtcaaataat cgctcattca gctcccactt cctctatcat ttcaattgat
    27181 gcccctaagg ctgttagttt ctcgataata tgatcataac cgcgtaagat ataatctaca
    27241 ttgtaaatcg tcgttgtccc ttctgccaat agtccagcaa tcactaaaca agcacctgcc
    27301 cgaagatcag aagccacgac ttctgtgccg tgaagtttat ttggaccatt cacgatgatc
    27361 atatttcctt ccacggtaat atcagcgccc attcgtgcta gttccggcac atggttcaca
    27421 cgtttcgcat agatcgtatc gatgatttct gaagtgcctg tagtcatcaa tagtaacgca
    27481 gtaagtggct gctgcagatc tgtggcaaat cctggataag gataggtcat gatgtttgcg
    27541 ggttttaatt ctttcgaggg gtaaacttcg atttcatctt cgctgatttt catatggaca
    27601 ccaatttcct gcaattttgc tataaagctt tccaaatgtt caaagatcac atttttcact
    27661 ttgataccat ttcctactgc agctgctaat gctagatagg ttcctgcttc gatgcggtca
    27721 ggaatcatga aatgccgaca gccatgtaat gtttcgactc cttcgatacg gatcacatct
    27781 gtacccgctc ctcgaacttt tgcccccata ttattcagta aagttgccac atctatgatt
    27841 tccggttctc gtgcagcgtt ttcaattact gtctgtcctt ttgcttttac agcagctaac
    27901 atcacattga tcgttgctcc gactgaaacc acgtccatga aaatccgatt gccacgcaat
    27961 cctttatttt ctgtccgtaa atacatggca ccatgttcat ttgtcacttc agcacctaga
    28021 gcttcaaatc ctttgacgtg taggtcaatc ggtctagggc ctaaataaca gccacctggc
    28081 aggcctacta ccgcttcgcc aaatttacca agcagtgttc ccataaaata ataagaagca
    28141 cgcaaactat tgatttttcc gtgcggcatc ggaatcgata cgacttctcg aggatcgatt
    28201 tctagtatgt tgttggaaaa atggacagtt gcgcccatga tctctaaaat ttcaattaat
    28261 gaatgaacat cttgtatgtc cggcacacct tccaagatca ctggggaatc agctaagatt
    28321 gcagctggaa tcaatgcgac tgcactattt tttgcaccgc tgatcgttac ttcaccagct
    28381 aaaggtcggt ttcctttgat tacgattttt ttcatctgtt tccaccttgt ttattaatct
    28441 tttttcatgg atatgcttgt attttatcat agataaaaaa aaagaaacaa gcatggtctg
    28501 ccgttttaag taaaaaagaa tggagagcga cttcaaaagt cgttctccat tcctatatat
    28561 cgtatctgtt atgtggatgc ttacgctttg ttagctgaac cgaaccagtc gatatgagat
    28621 ttagcatctt caacgattgc tgctttacct ggagctaata atttacgagg gtcgaatcct
    28681 ttaccttctt tatctttacc agcttcgata tatttacgag tagctgcagc aaatgataat
    28741 tggaattcag tgttaacatt tacttttgag atacccattg agatagcttt ttcgatttga
    28801 tcttgaggaa tacctgatcc gccatgtaat actaaaggca tgtctggacc tacagcatca
    28861 gcaattgctt gtaagtggtc aaaagctaat cctttccagt tttctggata ttgaccatgg
    28921 atgttaccga taccgcaagc taagtagtcg atacctgtag caaccatttg tttgcattct
    28981 tcaatatcag ctaattcgcc gttaccgatg attccgtctt cttctccgcc gattgaacca
    29041 acttcacatt caactgagat accttttgca tgtgcttttt caacaacgtc tttagctaat
    29101 ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt atagcctaat
    29161 tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta agtggatagc aactggaaca
    29221 gtgatgttca ttgcttcaac taaatcagtg attaaatctt tacagatttt gtaaccaccc
    29281 atgtatttag ctgcacccat tgatgtttgg ataagtactg gtgcttgttt tgcttcagcc
    29341 gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc gacagcgtaa
    29401 ccgcctttac gagcagcttt taaaaattca gctcctgata ctactggcat aataaaattc
    29461 ctcctaagtt ttgtaaaact aaatttgtta agacaattct taaccaacga ccctatttta
    29521 ccaaacattt cggtagtttt ctactaaaat caatgaaaat gaaaattttc aagaagtttt
    29581 ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa aagtcttttg ctttctatct
    29641 tttttcctac aaataataac aaatcagaaa aagagaaaag tgacaagaaa attgcttatc
    29701 cccttctctt gctacttttc tctttctatc aagttcaaaa attattttgt ttcgtgatct
    29761 gctaatgatg ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg acgagaaatc
    29821 aattctgggt ggaattggca tcccacaaag aattttttct ctggcagctc tacgatttct
    29881 actaaacggt tatctggtga gacacctgaa aagactagcc cttgttcttc aaacaattga
    29941 cgatatttgt tattgaactc atagcggtga cggtgacgtt cttgcacaac atcggctcca
    30001 tcatatgctt cagccgtctt cgttcctttt ttgattttac atgggtataa tccaaggcgt
    30061 aaagttccac ccaaattctc gatgttttcc tgatcagcca ttaaatcaat aatattgttt
    30121 gctgtatctg gattcgtttc agctgatgac gcatcttcta atccaacgac gttacgtgca
    30181 aattcgacac aagccatctg cataccaaga caaataccta aaaacggaac gtcattttcg
    30241 cgagcataac gaatcgcttc gattttccct tctactccgc gatcaccaaa tccaccagga
    30301 accaagattc catctgcgtc tttcaaacgt tcttctacat tttctctcgt taattcttgt
    30361 gctttgatcc aatcgatctc gatgtctgaa ttataggcga aaccagaatg tttcaatgct
    30421 tcaactactg agatatatgc atctggtaac tcgacatatt taccgacaag cgcaatccgt
    30481 acttttttct tcaagttcaa gacacgttct tctaattctt tccattctgt catgtctgct
    30541 tctggtgtat ctagttttaa atggtcgcaa acaatttgat ccattccttg tgcttgtaaa
    30601 actaatggga tggagtaaag tgtatctaca tctggcgact cgataactgc ttctggttcg
    30661 acatcacaaa attgtgccaa tttgtttttc acgctttgag aaacaggttg ctctgtacgt
    30721 accaccaaaa tattcggttg gatacctaaa ccacgtagct cttttacact gtgctgcgtt
    30781 ggtttagttt tcatttctcc agcagctttc aagtaaggaa tcaatgttgt atggatatac
    30841 ataacattat ctgtaccgac atctgctttc atttgacgaa gtgcttcaag gaatggcaaa
    30901 gattctatat cccctactgt tccccctact tctgtaatga tcacatctga atcagtcatt
    30961 ttagctgctc gcatgatttt ttctttgatc tcatttgtaa tatgaggaat aacttgaaca
    31021 gttgctccca agtattcgcc cttacgttct ttacgcaaaa cttcagaata gatttttcct
    31081 gtcgtcacgt tggagtattt gttcaaattg atgtcaataa aacgttcgta gtggcccaga
    31141 tccaaatctg tttcagcacc atcatctgtt acgaatactt ctccgtgctg atatgggctc
    31201 atcgtccccg gatccacgtt gatgtatgga tcaaattttt ggatagttac ttttaagccg
    31261 cggtttttca ataagcgtcc taaagaagct gcaacaatcc cttttccgat tgatgaaacg
    31321 acaccgccgg tcacaaaaat atattttgtc ataagttcaa aaactccttt ttaatttatg
    31381 tgcaggaaaa gtctcagaca ataaacaaag ctccctattc ttcaataaga atagggagcc
    31441 gaaatttctg taaacttctg accctctaaa agggtgccca aggtgtataa tacaagctga
    31501 ctaagggaaa gtcaagtact caaatgaata ctatccgctt acaatttttt ctttttcgat
    31561 atcctttgag aaattatttc ttaagtcctt tatgagtgca aaaaaaattt aacccacata
    31621 ataagcttga tataaacata gctaacgtta aacctaagca aatcagaaaa tcgttactga
    31681 aaaattttaa gtgaaataat agataaaaaa ataaaacaaa cattatgata gataatatcc
    31741 cacctaacaa agaactactt tttcctactt gcagcaacag tttatatagc ttatcgtaga
    31801 aacaatcttt ttctttcacc cttatttacc tcctttacgt aaaaaacgta gtccacagtt
    31861 ctcggatttt tggcatatct tttctattaa ataatatcaa aaacaaaaca attccactaa
    31921 ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg tagggattca ttgaaggtaa
    31981 cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct tatttctaaa
    32041 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccatc aaacatgaga
    32101 agctgttttt gaaattattt cttgtgattc ggagttaaat ggtaggctca caacctctgg
    32161 ataaacggta ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga
    32221 aagccatttt cacaaaattt ttcttaatac tcgaaactaa acgtcttttt caaaacctct
    32281 tggtaaacgg tgttcaaaag cttgcatctg cggtaataag ttcagccaat tcaaaaacaa
    32341 gaaaagctgt ttttgcttgg ctattcttat tacttgttgc agggcagctt tttcacaacc
    32401 tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    32461 atgaggaact atttttccgt tagtttctct aatactcagg tcaaaacgct gatttcacag
    32521 cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc
    32581 ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa
    32641 gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt
    32701 atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac
    32761 ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc
    32821 tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa
    32881 ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg
    32941 gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt
    33001 gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa
    33061 atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc
    33121 atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt
    33181 cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa
    33241 ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg
    33301 tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa
    33361 cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc
    33421 tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc
    33481 tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg
    33541 tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca
    33601 aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag
    33661 atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc
    33721 gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt
    33781 tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc
    33841 aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct
    33901 tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt
    33961 tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc
    34021 tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg
    34081 tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc
    34141 aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc
    34201 ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc
    34261 aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc
    34321 gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag
    34381 gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa
    34441 ggtatctagt tgattttcgt accagat
//
LOCUS       DAZKAO010000015        33865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000015 DAZKAO010000000
VERSION     DAZKAO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.015"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2056
                     /locus_tag="ACG8H8_000577"
     CDS             158..2056
                     /locus_tag="ACG8H8_000577"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP8985677.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2355..3074)
                     /locus_tag="ACG8H8_000578"
     CDS             complement(2355..3074)
                     /locus_tag="ACG8H8_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP8985678.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3264..3311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3411..3743
                     /locus_tag="ACG8H8_000579"
     CDS             3411..3743
                     /locus_tag="ACG8H8_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985679.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3730..5733
                     /locus_tag="ACG8H8_000580"
     CDS             3730..5733
                     /locus_tag="ACG8H8_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP8985680.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5752..6222
                     /locus_tag="ACG8H8_000581"
     CDS             5752..6222
                     /locus_tag="ACG8H8_000581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP8985681.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6243..6827
                     /locus_tag="ACG8H8_000582"
     CDS             6243..6827
                     /locus_tag="ACG8H8_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP8985682.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6842..7828
                     /locus_tag="ACG8H8_000583"
     CDS             6842..7828
                     /locus_tag="ACG8H8_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP8985683.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7818..8129
                     /locus_tag="ACG8H8_000584"
     CDS             7818..8129
                     /locus_tag="ACG8H8_000584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP8985684.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8151..9932
                     /locus_tag="ACG8H8_000585"
     CDS             8151..9932
                     /locus_tag="ACG8H8_000585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP8985685.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9925..11301
                     /locus_tag="ACG8H8_000586"
     CDS             9925..11301
                     /locus_tag="ACG8H8_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP8985686.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11308..11943
                     /locus_tag="ACG8H8_000587"
     CDS             11308..11943
                     /locus_tag="ACG8H8_000587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP8985687.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11973..13328
                     /locus_tag="ACG8H8_000588"
     CDS             11973..13328
                     /locus_tag="ACG8H8_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP8985688.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13520..14914)
                     /locus_tag="ACG8H8_000589"
     CDS             complement(13520..14914)
                     /locus_tag="ACG8H8_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP8985689.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            15006..15977
                     /locus_tag="ACG8H8_000590"
     CDS             15006..15977
                     /locus_tag="ACG8H8_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP8985690.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15974..16756
                     /locus_tag="ACG8H8_000591"
     CDS             15974..16756
                     /locus_tag="ACG8H8_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP8985691.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16925..17296)
                     /locus_tag="ACG8H8_000592"
     CDS             complement(16925..17296)
                     /locus_tag="ACG8H8_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8985692.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17430..17873
                     /locus_tag="ACG8H8_000593"
     CDS             17430..17873
                     /locus_tag="ACG8H8_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP8985693.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17916..18725)
                     /gene="yidA"
                     /locus_tag="ACG8H8_000594"
     CDS             complement(17916..18725)
                     /gene="yidA"
                     /locus_tag="ACG8H8_000594"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP8985694.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18744..20111)
                     /locus_tag="ACG8H8_000595"
     CDS             complement(18744..20111)
                     /locus_tag="ACG8H8_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8985695.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20095..20925)
                     /locus_tag="ACG8H8_000596"
     CDS             complement(20095..20925)
                     /locus_tag="ACG8H8_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP8985696.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21088..21525
                     /locus_tag="ACG8H8_000597"
     CDS             21088..21525
                     /locus_tag="ACG8H8_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP8985697.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21601..22221
                     /gene="rpoE"
                     /locus_tag="ACG8H8_000598"
     CDS             21601..22221
                     /gene="rpoE"
                     /locus_tag="ACG8H8_000598"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP8985698.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22410..22916
                     /locus_tag="ACG8H8_000599"
     CDS             22410..22916
                     /locus_tag="ACG8H8_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP8985699.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22933..23832
                     /locus_tag="ACG8H8_000600"
     CDS             22933..23832
                     /locus_tag="ACG8H8_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP8985700.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23919..24803
                     /locus_tag="ACG8H8_000601"
     CDS             23919..24803
                     /locus_tag="ACG8H8_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP8985701.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24995..25066)
                     /locus_tag="ACG8H8_000602"
     tRNA            complement(24995..25066)
                     /locus_tag="ACG8H8_000602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25031..25033),aa:Arg,seq:ccg)
     gene            25126..26460
                     /gene="rpoN"
                     /locus_tag="ACG8H8_000603"
     CDS             25126..26460
                     /gene="rpoN"
                     /locus_tag="ACG8H8_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP8985702.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26609..27700
                     /locus_tag="ACG8H8_000604"
     CDS             26609..27700
                     /locus_tag="ACG8H8_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP8985703.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27741..28742
                     /gene="gap"
                     /locus_tag="ACG8H8_000605"
     CDS             27741..28742
                     /gene="gap"
                     /locus_tag="ACG8H8_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP8985704.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28852..30042
                     /gene="pgk"
                     /locus_tag="ACG8H8_000606"
     CDS             28852..30042
                     /gene="pgk"
                     /locus_tag="ACG8H8_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP8985705.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30146..30901
                     /gene="tpiA"
                     /locus_tag="ACG8H8_000607"
     CDS             30146..30901
                     /gene="tpiA"
                     /locus_tag="ACG8H8_000607"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP8985706.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31017..32315
                     /gene="eno"
                     /locus_tag="ACG8H8_000608"
     CDS             31017..32315
                     /gene="eno"
                     /locus_tag="ACG8H8_000608"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP8985707.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32714..33853
                     /locus_tag="ACG8H8_000609"
     CDS             32714..33853
                     /locus_tag="ACG8H8_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP8985708.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11187 a         5996 c         7210 g         9472 t
ORIGIN      
        1 agaaaaatat agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct
       61 atttctattt ttttattata ttggatatat atattcattg aaaatagagc ataacaactt
      121 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
      181 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
      241 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
      301 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
      361 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
      421 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
      481 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
      541 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
      601 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
      661 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
      721 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
      781 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
      841 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
      901 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
      961 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
     1021 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
     1081 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
     1141 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
     1201 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
     1261 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
     1321 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
     1381 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
     1441 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
     1501 tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa
     1561 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
     1621 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
     1681 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
     1741 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
     1801 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
     1861 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
     1921 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
     1981 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
     2041 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
     2101 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
     2161 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
     2221 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
     2281 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
     2341 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
     2401 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
     2461 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
     2521 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
     2581 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
     2641 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
     2701 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
     2761 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
     2821 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
     2881 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
     2941 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
     3001 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
     3061 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
     3121 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
     3181 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
     3241 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
     3301 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
     3361 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
     3421 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
     3481 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
     3541 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
     3601 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
     3661 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
     3721 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
     3781 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
     3841 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
     3901 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
     3961 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
     4021 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
     4081 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
     4141 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
     4201 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
     4261 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
     4321 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
     4381 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
     4441 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
     4501 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
     4561 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
     4621 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
     4681 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
     4741 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
     4801 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
     4861 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
     4921 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
     4981 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
     5041 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
     5101 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
     5161 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
     5221 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
     5281 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
     5341 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
     5401 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
     5461 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
     5521 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
     5581 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
     5641 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
     5701 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
     5761 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
     5821 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
     5881 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
     5941 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
     6001 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
     6061 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
     6121 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
     6181 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
     6241 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
     6301 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
     6361 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
     6421 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
     6481 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
     6541 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
     6601 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
     6661 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
     6721 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
     6781 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
     6841 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
     6901 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
     6961 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
     7021 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
     7081 cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga
     7141 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
     7201 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
     7261 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
     7321 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
     7381 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
     7441 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
     7501 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
     7561 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct
     7621 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
     7681 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
     7741 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa
     7801 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
     7861 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
     7921 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
     7981 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
     8041 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
     8101 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
     8161 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
     8221 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
     8281 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
     8341 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
     8401 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
     8461 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
     8521 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
     8581 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
     8641 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
     8701 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
     8761 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
     8821 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
     8881 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
     8941 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
     9001 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
     9061 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
     9121 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
     9181 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
     9241 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
     9301 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
     9361 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
     9421 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
     9481 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
     9541 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
     9601 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
     9661 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
     9721 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
     9781 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
     9841 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
     9901 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
     9961 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    10021 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    10081 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    10141 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    10201 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    10261 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    10321 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    10381 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    10441 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    10501 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    10561 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    10621 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    10681 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    10741 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    10801 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    10861 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    10921 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    10981 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    11041 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    11101 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    11161 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    11221 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    11281 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    11341 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    11401 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    11461 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    11521 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    11581 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    11641 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    11701 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    11761 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    11821 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    11881 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    11941 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    12001 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    12061 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    12121 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    12181 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    12241 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    12301 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    12361 tgctttacat cctaaaattg gcctttgtta tccaattgat tggtacggtt gctttatctt
    12421 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    12481 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    12541 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    12601 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    12661 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    12721 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    12781 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    12841 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    12901 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    12961 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    13021 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    13081 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    13141 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    13201 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    13261 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    13321 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    13381 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    13441 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    13501 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    13561 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    13621 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    13681 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    13741 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    13801 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    13861 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    13921 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    13981 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    14041 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    14101 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    14161 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    14221 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    14281 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    14341 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    14401 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    14461 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    14521 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    14581 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    14641 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    14701 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    14761 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    14821 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    14881 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    14941 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    15001 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    15061 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    15121 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    15181 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    15241 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    15301 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    15361 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    15421 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    15481 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    15541 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    15601 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    15661 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    15721 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    15781 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    15841 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    15901 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    15961 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    16021 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    16081 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    16141 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    16201 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    16261 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    16321 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    16381 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    16441 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    16501 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    16561 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    16621 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    16681 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    16741 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    16801 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    16861 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    16921 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    16981 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccggca gctttatgtt
    17041 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    17101 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    17161 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    17221 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    17281 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    17341 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    17401 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    17461 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    17521 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    17581 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    17641 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    17701 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    17761 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    17821 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    17881 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    17941 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    18001 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    18061 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    18121 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    18181 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    18241 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    18301 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    18361 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    18421 cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    18481 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    18541 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    18601 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    18661 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    18721 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    18781 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    18841 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    18901 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    18961 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    19021 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    19081 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    19141 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    19201 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    19261 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    19321 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    19381 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    19441 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    19501 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    19561 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    19621 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    19681 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    19741 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    19801 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    19861 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    19921 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    19981 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    20041 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    20101 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    20161 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    20221 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    20281 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    20341 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg
    20401 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    20461 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    20521 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    20581 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    20641 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    20701 gatcactgga gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt
    20761 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    20821 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    20881 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    20941 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    21001 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    21061 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    21121 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    21181 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    21241 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    21301 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    21361 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    21421 ttgaaagacc gtcccacagg agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    21481 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    21541 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    21601 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    21661 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    21721 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    21781 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    21841 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    21901 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    21961 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    22021 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    22081 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    22141 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    22201 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    22261 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
    22321 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
    22381 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
    22441 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
    22501 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
    22561 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
    22621 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
    22681 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
    22741 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
    22801 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
    22861 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
    22921 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
    22981 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
    23041 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
    23101 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
    23161 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
    23221 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
    23281 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
    23341 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
    23401 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
    23461 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
    23521 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
    23581 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
    23641 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
    23701 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
    23761 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
    23821 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
    23881 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
    23941 tcagtattat tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga
    24001 tcaccgaagt aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat
    24061 cagaacaatc aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc
    24121 aagaatcagc ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa
    24181 cgagccgaga tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga
    24241 ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg
    24301 tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc
    24361 acgagattgg caattacaca tacgatcatc agaatctggc cactctttct gcccagtcga
    24421 tgacggagga agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca
    24481 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
    24541 cgatcatcga atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt
    24601 tgcagaagat ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc
    24661 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca
    24721 tcacgacagt tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa
    24781 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
    24841 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacaggtaca
    24901 acaaaaaact gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca
    24961 actattcgta aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg
    25021 tctcaagaac cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa
    25081 ttgtaactaa acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa
    25141 aatttttcac aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag
    25201 tccatccaag ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca
    25261 atggaaaatc cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct
    25321 tcttcatctt cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa
    25381 gggtctttat ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg
    25441 aggaaaatcg ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt
    25501 ttagaagaag ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact
    25561 ctgattcagc aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg
    25621 cttcaaacag aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt
    25681 tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta
    25741 catgctgttc agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt
    25801 ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat
    25861 aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt
    25921 tacttcaaac aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg
    25981 aaacaggaat tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg
    26041 gttgctcaag tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc
    26101 aagcctctta cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt
    26161 cgtgcagtca acgggaaata tttggaaaca gatttcggtg tatttgaatt aaaaaaattt
    26221 ttcactacac gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca
    26281 gcgaagaaaa agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtcggac
    26341 cagaaattgg ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca
    26401 aaatatcgtg atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa
    26461 tcagtcaaaa gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag
    26521 agcgaaactg ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc
    26581 gaatagttaa tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa
    26641 gtgtccaact aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc
    26701 agatattatc gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca
    26761 gccgattggg cgtcgaagtt tatcggaaag tatggggatc acagaacgag tcttacgaac
    26821 agaaacagac cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct
    26881 gacaactaag ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg
    26941 tacgcatcaa accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt
    27001 ttcaggaaac agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga
    27061 agccttagac cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac
    27121 tatggctgtg gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt
    27181 tgtccctgca agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc
    27241 aagaatggct gctaaagcaa acggtaacca ccgcgcactt tatgttccgg aacaattaag
    27301 tctggcaact tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat
    27361 tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg
    27421 taagatgact gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt
    27481 tggctacttt ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga
    27541 attgcaagat ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc
    27601 taaagcaatt cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga
    27661 agctgctgca aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt
    27721 tcataaggag gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg
    27781 gacgtctagc attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg
    27841 acttgacaga tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt
    27901 tcaacggaac agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag
    27961 tattagctaa ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc
    28021 tagaatgtac tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg
    28081 ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca
    28141 caaaccacga aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta
    28201 actgtttagc tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa
    28261 tgacaactat ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag
    28321 gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg
    28381 ctaaagctat cggtttagta attcccgaat tgaacggtaa attagacgga gctgctcaac
    28441 gtgttcctgt accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa
    28501 ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca
    28561 cagacgaaat cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta
    28621 cacaaactaa agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg
    28681 acaacgaaat gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat
    28741 aagatagaca aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa
    28801 tcgcgcttct tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag
    28861 aaaacagtga aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac
    28921 gttcctttga aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca
    28981 atcaactacg ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta
    29041 aaaacagaag aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa
    29101 ttattaggta aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca
    29161 atccgcaaca tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt
    29221 gacggtaaaa aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc
    29281 gatgtatttg ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt
    29341 atcgcttcaa ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc
    29401 atcggtgaag cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa
    29461 gtttctgata aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc
    29521 ggtggcggaa tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta
    29581 gtagaagctg ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt
    29641 gtcttgccaa tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta
    29701 atcgaaggag acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa
    29761 ttatttgctg atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta
    29821 tttgaaatga gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta
    29881 gaaggcgcaa caacaatcat cggtggcgga gactcagccg cagcagcaga acaattagga
    29941 tttgctgaca aattcacaca catctcaaca ggtggcggcg caagcttgga attgcttgaa
    30001 ggtaaagaat taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa
    30061 ttctgacgag taatcgaaca aaaggtgaag ggagttttca tgactcccta gccatttttc
    30121 aataaattaa ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat
    30181 gaacaaaact gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc
    30241 aaatgacaaa gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac
    30301 atcagctgaa ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg
    30361 tgcgttcact ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat
    30421 catcggacat tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa
    30481 agcgaaagct atctttgcaa atggtatgac accaatccta tgctgcggcg aatctttaga
    30541 aacctatgaa gcaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc
    30601 tgatctttca gctgaacagg tgtcaaacat ggttattgct tatgaaccaa tctgggcaat
    30661 cggtactggt aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca
    30721 aacagttgaa aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg
    30781 ttctgtaaaa ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt
    30841 agtcggtggc gcaagcttgc aagcagattc attcttgtca ttgttagacg ctgtaaaata
    30901 attgttgaaa atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa
    30961 aatcgtatat gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt
    31021 caattattac tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg
    31081 aagtagaagt atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt
    31141 caactggtga atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta
    31201 aaggtgtaac taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct
    31261 acgatgttcg tgaccaaatg gctatcgaca aagctatgat cgctttagac ggaactccta
    31321 acaaaggtaa attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag
    31381 ctgctgacta cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat
    31441 tgccaacacc aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact
    31501 tccaagaatt catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg
    31561 gtgctgaagt attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg
    31621 ttggtgacga aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa
    31681 tcatcgaagc aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg
    31741 atgctgcttc ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgactcag
    31801 gcgaaggcga aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat
    31861 atccaatcat ctcaatcgaa gatggattag acgaaaacga ctgggacgga ttcaagaaat
    31921 taactgacgt attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca
    31981 ctcaaaaatt atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga
    32041 accaaatcgg tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct
    32101 acacagcagt tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg
    32161 ctgttgcaac aaacgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg
    32221 ctaaatacaa ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag
    32281 gtttgaaatc tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat
    32341 cgactattga aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc
    32401 tgaaatatca gcaggtagag agtttttttg caactagctt ctgtatatga tgtttacaaa
    32461 gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat
    32521 aagagctttt aaggatagaa cagtagaata ggaatctatg ggcaagtcct ctcgtataaa
    32581 taaaatagga gggaagtagc agcatatttt tcataaagtg gctctctttc ttcattttta
    32641 ctttggttgc tcctatttga aaattcgagc acactcaaat acaataaaaa gaaatggcaa
    32701 gggagaggat tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga ttatccgaat
    32761 taaatcagtg aatggaaacg aaggtgaagt agcagcctat ttgaataagc ttttggcaaa
    32821 gcatgacata acaggagaga ttgtttctta cagagatggt agagataatt tgattgctcg
    32881 ttaccaaaag ggccaatcag gaaaagtgtt aggcctatct ggacatatgg atgtcgtggc
    32941 ggctggtgat gaatcgtctt ggacctatgc accctttgca gcagaaatcc atgggaaccg
    33001 tttgtacgga cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg tgattgccat
    33061 gatcgaactg aaagagtcag gaaaaccatt taacggaaca gtcaaattat tagccactgt
    33121 cggagaagaa gtgggtgaac taggtgggga acagctcacg aaagcaggct atgtagatga
    33181 cttagatgcc ttgatcatcg gagagccaac caattacagt ttgatgtata cgcacatggg
    33241 atcaatcaac tatacagtca cctcgcacgg aaaagaagcg catagctcta tgccagacca
    33301 aggttataat gcaatcaatc acctaaatga attcattacg aaagcaaatg ctgaaatgaa
    33361 tcacctcgct gaaacaatcg agaatcctgt cctaggaaaa acaatccata atgttacatt
    33421 gatcagtggc gggaatcaag tcaacagcat cccaagccat gcgcagctcc aagggaacat
    33481 tcgatcgatt ccagaatacc caaatgataa aatcattgca ttgctgcaat caatcgtcaa
    33541 tgaattgaat caagagacag actatcattt ggaattgatg atcgattaca acaaaatacc
    33601 tgtcaaagct gatccagatt ctcctttgat tcattgcatt cagcaacaat tcagccagcc
    33661 attaccttta gttggagcgg cagcaacaac ggatgcagcc gaatttacga aagctaacca
    33721 ttccttcgac tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc aagtagatga
    33781 gtatgttgaa atcgataatt atttggatat gatcgaaaaa tatcaaggaa ttattttatc
    33841 ctacttagcg taagattttt ttaat
//
LOCUS       DAZKAO010000016        33649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000016 DAZKAO010000000
VERSION     DAZKAO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.016"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            228..2111
                     /locus_tag="ACG8H8_000610"
     CDS             228..2111
                     /locus_tag="ACG8H8_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8985709.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            2123..3070
                     /locus_tag="ACG8H8_000611"
     CDS             2123..3070
                     /locus_tag="ACG8H8_000611"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP8985710.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3090..3617
                     /locus_tag="ACG8H8_000612"
     CDS             3090..3617
                     /locus_tag="ACG8H8_000612"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP8985711.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3680..4333)
                     /locus_tag="ACG8H8_000613"
     CDS             complement(3680..4333)
                     /locus_tag="ACG8H8_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP8985712.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4465..5307
                     /locus_tag="ACG8H8_000614"
     CDS             4465..5307
                     /locus_tag="ACG8H8_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8985713.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5465..6361
                     /locus_tag="ACG8H8_000615"
     CDS             5465..6361
                     /locus_tag="ACG8H8_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8985714.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6364..7077
                     /locus_tag="ACG8H8_000616"
     CDS             6364..7077
                     /locus_tag="ACG8H8_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP8985715.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7097..7615
                     /gene="msrA"
                     /locus_tag="ACG8H8_000617"
     CDS             7097..7615
                     /gene="msrA"
                     /locus_tag="ACG8H8_000617"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8985716.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7612..7839
                     /locus_tag="ACG8H8_000618"
     CDS             7612..7839
                     /locus_tag="ACG8H8_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP8985717.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7991..8542
                     /gene="lepB"
                     /locus_tag="ACG8H8_000619"
     CDS             7991..8542
                     /gene="lepB"
                     /locus_tag="ACG8H8_000619"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8985718.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8625..9167)
                     /locus_tag="ACG8H8_000620"
     CDS             complement(8625..9167)
                     /locus_tag="ACG8H8_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP8985719.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9404..9802)
                     /locus_tag="ACG8H8_000621"
     CDS             complement(9404..9802)
                     /locus_tag="ACG8H8_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8985720.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            10062..10922
                     /gene="ylqF"
                     /locus_tag="ACG8H8_000622"
     CDS             10062..10922
                     /gene="ylqF"
                     /locus_tag="ACG8H8_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP8985721.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            10915..11682
                     /locus_tag="ACG8H8_000623"
     CDS             10915..11682
                     /locus_tag="ACG8H8_000623"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP8985722.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11738..12595
                     /gene="dprA"
                     /locus_tag="ACG8H8_000624"
     CDS             11738..12595
                     /gene="dprA"
                     /locus_tag="ACG8H8_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP8985723.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12717..14795
                     /gene="topA"
                     /locus_tag="ACG8H8_000625"
     CDS             12717..14795
                     /gene="topA"
                     /locus_tag="ACG8H8_000625"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP8985724.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14764..16140
                     /gene="trmFO"
                     /locus_tag="ACG8H8_000626"
     CDS             14764..16140
                     /gene="trmFO"
                     /locus_tag="ACG8H8_000626"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP8985725.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16338..17243
                     /gene="xerC"
                     /locus_tag="ACG8H8_000627"
     CDS             16338..17243
                     /gene="xerC"
                     /locus_tag="ACG8H8_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP8985726.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17280..17828
                     /gene="hslV"
                     /locus_tag="ACG8H8_000628"
     CDS             17280..17828
                     /gene="hslV"
                     /locus_tag="ACG8H8_000628"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP8985727.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            17842..19242
                     /gene="hslU"
                     /locus_tag="ACG8H8_000629"
     CDS             17842..19242
                     /gene="hslU"
                     /locus_tag="ACG8H8_000629"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP8985728.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19260..20051
                     /gene="codY"
                     /locus_tag="ACG8H8_000630"
     CDS             19260..20051
                     /gene="codY"
                     /locus_tag="ACG8H8_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP8985729.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20133..21008
                     /locus_tag="ACG8H8_000631"
     CDS             20133..21008
                     /locus_tag="ACG8H8_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP8985730.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(21140..21778)
                     /gene="plsY"
                     /locus_tag="ACG8H8_000632"
     CDS             complement(21140..21778)
                     /gene="plsY"
                     /locus_tag="ACG8H8_000632"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP8985731.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            21905..22339
                     /locus_tag="ACG8H8_000633"
     CDS             21905..22339
                     /locus_tag="ACG8H8_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP8985732.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            22505..24550
                     /gene="parE"
                     /locus_tag="ACG8H8_000634"
     CDS             22505..24550
                     /gene="parE"
                     /locus_tag="ACG8H8_000634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP8985733.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            24562..27012
                     /gene="parC"
                     /locus_tag="ACG8H8_000635"
     CDS             24562..27012
                     /gene="parC"
                     /locus_tag="ACG8H8_000635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP8985734.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            27289..29520
                     /gene="pflB"
                     /locus_tag="ACG8H8_000636"
     CDS             27289..29520
                     /gene="pflB"
                     /locus_tag="ACG8H8_000636"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP8985735.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            29764..30525
                     /gene="pflA"
                     /locus_tag="ACG8H8_000637"
     CDS             29764..30525
                     /gene="pflA"
                     /locus_tag="ACG8H8_000637"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP8985736.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            30610..31536
                     /locus_tag="ACG8H8_000638"
     CDS             30610..31536
                     /locus_tag="ACG8H8_000638"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP8985737.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            31718..32611
                     /locus_tag="ACG8H8_000639"
     CDS             31718..32611
                     /locus_tag="ACG8H8_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP8985738.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            32745..32870
                     /locus_tag="ACG8H8_000640"
     CDS             32745..32870
                     /locus_tag="ACG8H8_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP8985739.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(33004..33636)
                     /locus_tag="ACG8H8_000641"
     CDS             complement(33004..33636)
                     /locus_tag="ACG8H8_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985740.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        11926 a         5807 c         7057 g         8859 t
ORIGIN      
        1 ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa
       61 aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga
      121 atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga
      181 agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa
      241 aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt
      301 tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc
      361 tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc
      421 caactgatta ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg
      481 ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg
      541 aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta
      601 aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta
      661 ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc
      721 agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag
      781 acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac
      841 aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa
      901 accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt
      961 acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc
     1021 ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc
     1081 agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag
     1141 aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca
     1201 aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc
     1261 aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta
     1321 atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc
     1381 atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt
     1441 acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag
     1501 aattacttga acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt
     1561 caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt
     1621 tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt
     1681 attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca
     1741 aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa
     1801 acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac
     1861 caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag
     1921 aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag
     1981 aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat
     2041 ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg
     2101 tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt
     2161 ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata
     2221 ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt
     2281 tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct
     2341 attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc
     2401 agactataat ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt
     2461 caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc
     2521 ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga
     2581 aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac
     2641 tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc
     2701 tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca
     2761 attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc
     2821 gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac
     2881 attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg
     2941 agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt
     3001 tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat
     3061 cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa
     3121 acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct
     3181 tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg
     3241 gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag
     3301 cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag
     3361 acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta
     3421 cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg
     3481 ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc
     3541 gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag
     3601 gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc
     3661 catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac
     3721 ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa
     3781 agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc
     3841 caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga
     3901 tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa
     3961 tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga
     4021 tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat
     4081 cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg
     4141 ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata tggagggagg
     4201 aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc
     4261 caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt
     4321 tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg
     4381 tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg
     4441 ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt
     4501 acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg
     4561 atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg
     4621 atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg
     4681 atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc
     4741 ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg
     4801 gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa
     4861 ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac
     4921 aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc
     4981 agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc
     5041 cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt
     5101 aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat
     5161 gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac
     5221 atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg
     5281 tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc
     5341 ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata
     5401 aagactacct ttttgataaa atttttgata acattagaac caatcgaata caagaggtga
     5461 aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat
     5521 catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt
     5581 aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga
     5641 ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc
     5701 aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa
     5761 tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa
     5821 tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat
     5881 ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca
     5941 ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta
     6001 cattttaggc atctacaatc cattttattt gaactttcct gagatcactg acatgcagac
     6061 aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta
     6121 ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga
     6181 agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt
     6241 gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc
     6301 agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata
     6361 aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa
     6421 ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt
     6481 atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga
     6541 gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc
     6601 cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca
     6661 ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt atattttgat
     6721 ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca
     6781 ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat
     6841 tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg
     6901 caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt
     6961 agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact
     7021 agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat
     7081 tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat
     7141 ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca
     7201 tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt
     7261 ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca
     7321 gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc
     7381 ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt
     7441 agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt
     7501 ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag
     7561 aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga
     7621 agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt
     7681 ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa
     7741 atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa
     7801 gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt gtactttatg
     7861 atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct
     7921 agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga
     7981 ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata
     8041 cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc
     8101 agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa
     8161 attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta
     8221 tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa
     8281 gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc
     8341 aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc
     8401 ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt
     8461 tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga
     8521 tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct
     8581 ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta
     8641 aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg
     8701 atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac
     8761 aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg
     8821 gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat
     8881 ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg
     8941 aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa
     9001 cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct
     9061 atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca
     9121 ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct
     9181 ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga
     9241 tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg
     9301 taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg
     9361 cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata
     9421 cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag
     9481 gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt
     9541 tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt gtttttttat
     9601 tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta
     9661 tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat tcttcatttt
     9721 ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca acaggaaaat
     9781 taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg tctatcatct
     9841 cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa ataaaaattt
     9901 gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc acttaactaa
     9961 tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa aaaacaagta
    10021 aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc caatggtttc
    10081 cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat gtggacatcg
    10141 tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg ctagatcaga
    10201 tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca gatccacaac
    10261 agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta gctatcacgg
    10321 cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca ttgaaagaaa
    10381 aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca atggtcttag
    10441 gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag aaaattgcac
    10501 agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta ggtaatgagt
    10561 tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa gaaatcggca
    10621 aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat gatttagcga
    10681 tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat cgttatggtt
    10741 tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt acagcaaaac
    10801 gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat attcgtcaag
    10861 gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa agtgatgact
    10921 gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga tgatcctaca
    10981 ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc ttcttttgaa
    11041 cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt ttgctttgaa
    11101 aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt aggaagagga
    11161 ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga gatcatatta
    11221 gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta tcaacaagtt
    11281 caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat cgatgatgtg
    11341 aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt gtccattgta
    11401 ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca ggaaaaaata
    11461 atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc taaagtatat
    11521 cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt ctcgcataat
    11581 gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat tacgccaatc
    11641 catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc cgcttttacg
    11701 tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag aagaaaattt
    11761 attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac ttgctaactg
    11821 gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga taggtaaaat
    11881 aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta actggggcaa
    11941 aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc ctcagctgct
    12001 tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt tactttcttg
    12061 taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa aaacagttta
    12121 ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct gcgcgaaagg
    12181 gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa tcgcagtcat
    12241 tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag aggtgattgc
    12301 aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa agcattgttt
    12361 tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct ttgaagccaa
    12421 gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa gaacagtttt
    12481 ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt tgatccaaga
    12541 cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag aataacacca
    12601 tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta gctacgattt
    12661 gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat tcagtaatgg
    12721 cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt gagaaatatc
    12781 ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta ccgaaaagta
    12841 aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata cgcggaaaag
    12901 gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt tacttggcaa
    12961 gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta cttgggttgg
    13021 atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca gtcaaagcag
    13081 cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa gctcgtcgta
    13141 ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag gtcaagaaag
    13201 ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac cgtgaaaaag
    13261 agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt aaaaaagctc
    13321 gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa ttaccagatg
    13381 cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa gttaaaaaag
    13441 tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt agcctgcaac
    13501 aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta gcacagcaat
    13561 tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca tacatgcgta
    13621 ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt attgaagata
    13681 cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct caaggcgcac
    13741 aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat gaactcaaac
    13801 aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt tttgtggcta
    13861 gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa aatggtgtca
    13921 tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg tatgtggaag
    13981 gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa gaaggcgatg
    14041 tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca gcacgtttta
    14101 gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct tccacttatg
    14161 caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa aaaagatttg
    14221 agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc cctcaaatcg
    14281 tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa gaaggcacgg
    14341 aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa ctgacgaatg
    14401 cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc gattgtgatg
    14461 tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat gcttgcagta
    14521 attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg acttgcccag
    14581 tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta ttctatggtt
    14641 gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc agaaattgtc
    14701 caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa gtagtctgtg
    14761 taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct ttcattgaaa
    14821 atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca gatcgcacaa
    14881 gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga ggcgcatcac
    14941 acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag tttggctaat
    15001 gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat cacttcggca
    15061 gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc tttttctgaa
    15121 acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga agaaattact
    15181 gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca tccattagca
    15241 gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc agctgcacca
    15301 atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg ctatgacaaa
    15361 ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc tttccatgag
    15421 gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa atattttgaa
    15481 ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct tttcggacct
    15541 atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc tgttatccag
    15601 ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca gactcacttg
    15661 aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa tgctgaattc
    15721 gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt gctgaaaccg
    15781 acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac tggtgtagaa
    15841 ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc acgcttagca
    15901 aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat ggcttactac
    15961 attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg tctgtttcct
    16021 gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc acaacgagct
    16081 ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc aaatgagtaa
    16141 ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg tttttcttaa
    16201 gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta gaaatattgg
    16261 ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat tctctgggac
    16321 aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg cgttatttga
    16381 ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac attctccatt
    16441 tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa tattttacgg
    16501 ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga aattctatca
    16561 gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa gaagtgataa
    16621 aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg ccacgttttt
    16681 tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt cctttggatt
    16741 tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta agtgagtgta
    16801 gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc catggtaaag
    16861 ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg caagaatatt
    16921 tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat atcgtttttg
    16981 tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg aatcaaatga
    17041 tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat acatttgcta
    17101 ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta ggacatgcca
    17161 atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa aaaaactatc
    17221 gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag gaaacaaaca
    17281 tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac gggaaatttg
    17341 ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa ggcactgcaa
    17401 agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga agtgttgcag
    17461 atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac gggaatctac
    17521 aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg caaaaacttg
    17581 aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt acaggagaag
    17641 ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt gcgttggctg
    17701 cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa attgcagaaa
    17761 atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt atcgtagaag
    17821 aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca aatcgtaagt
    17881 gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc cgttgctttg
    17941 cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat tacaccaaaa
    18001 aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg acgtttggca
    18061 aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga agtcggttat
    18121 gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat ccaaatcgta
    18181 gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga aaaacgtttg
    18241 gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa tcaatttgaa
    18301 caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca agaagaagtg
    18361 acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa gggtttactg
    18421 gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc aatgatgaac
    18481 aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt gacaccgaag
    18541 aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat caaggaagaa
    18601 tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt agcggaagaa
    18661 agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca gcaatctgga
    18721 gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg ttcacaagta
    18781 aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc aggagctttc
    18841 catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc gatacgggta
    18901 gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc aaataatgct
    18961 ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac gtttacaaaa
    19021 gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac agataatatc
    19081 ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt atttgaagca
    19141 ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga caaacttggc
    19201 tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg aggacgacaa
    19261 tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa aagaatactt
    19321 ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt ggtattcttg
    19381 acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat gaacgtcacg
    19441 atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct gaatcttata
    19501 ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact agtgatatta
    19561 ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact gtcgttccga
    19621 tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa caatcgtttg
    19681 cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg cagatccttt
    19741 atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc caaatggcaa
    19801 tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa gcattggatg
    19861 gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc actcgttctg
    19921 tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct cgttcgttag
    19981 gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag ttagcaaaac
    20041 acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag ccttcccatt
    20101 ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa aatgaccagc
    20161 tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca aaagaacatg
    20221 gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca ccggttttat
    20281 tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa acatatcaaa
    20341 tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat ggacaagaaa
    20401 tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca tttgattttg
    20461 aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat caagtggcaa
    20521 ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc aatgtacctc
    20581 ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg aaatcaagat
    20641 tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta ggtcagacaa
    20701 atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt tttgaaacaa
    20761 ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta actttaagct
    20821 ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg cctttcgttt
    20881 gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac ttgatggaaa
    20941 aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat tccatcacag
    21001 tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa ggtgtgtaaa
    21061 aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtcacactc ccgttttatc
    21121 tctttggaat gaaaaagtat caggattttt tcttgctgct attcaaacca aacgggattc
    21181 gactttcagt atggtttctg atacgcttga tattgtcttt atgccgataa aagataaagg
    21241 aagaaactaa aaaggcaatg atcgtcagta accaattggg cttagccaaa atcacaggag
    21301 ctacataagg aaggacgatc gtagataaag tgatcaatac tgcactgatc atactgctca
    21361 aactcaccat acttgtacaa aataaactaa cgataaagat gatacaagaa taaacaaaaa
    21421 atacaggatt aaatcctaaa agcattcctg cacttgtcgc aacagctttt cctcctttga
    21481 acccagcaaa aatcggaaat gtatggccaa gaatggcgca taccccaaac caaagagggt
    21541 taatcccaga aaggtggaag atcatcggca aacaagtagc caatgtacct tttaaaatat
    21601 ccataagcag gacaacaatc ccggctgttt tgcctaatac acgaaatgta ttggtcgttc
    21661 cagtattacc gctcccgtgt tgtcggatat cttttttgaa aaacaatttg ccaatccaga
    21721 caccggaagg aatagaacct aaaagatatg ctaaaataag taataagatc aatttcattt
    21781 tttaccctct ttcaagttga aaactatcct ttattttagc atgaataggt aacaggaaca
    21841 attgaaaaaa tgaaagatac aagtataata gatggaataa aaagaaaagc aaggggtggc
    21901 agaaatgtct gtaaaaaatc caacacaaga aacgattttc aactatttgg aagaagcaaa
    21961 aaatattgcc gtcgtaggct tgagcaataa accagaacga acaagttaca tggttgcaca
    22021 ggtattgcaa gacaatggat ataaaatcat accagtaaat ccattattgg ctggtgaaga
    22081 aattctaggc gagaaagtct atgcgaattt gacagaaatc gatgatccaa tcgatatcgt
    22141 agatgtattt agacggagtg aatttttacc tgaaatagca ggtgattttt tgaaaactga
    22201 tgctaaagtg ttctgggcac aattagggat tgaaaacaaa gaagcagctg agatgctaag
    22261 aaaagcagga cgtacagata tcgtaatgga tcgttgtatt aagattgaac ttggtaagtt
    22321 gaaagaaaac caaaaataaa aatgaccaca aagaagagat gatcgataga ggggaatgtc
    22381 gaaaaagcgg ttaggagata attaaaattg atttctcgtc taccctcttt tttttcacga
    22441 ccttttctag tatgataatc gtggtgcggt tttgccgcaa agtattattg aaggagcgtt
    22501 tgccttggca aaaaaagtaa acaacgaata caatgactcg tccatccaag tattggaagg
    22561 attagaggca gttagaaaaa gaccagggat gtacattgga tccactgaca gccgaggctt
    22621 gcatcattta gtctatgaaa tcgtcgataa tgcagttgac gaagcattat caggttatgg
    22681 cagtgagatt gatgtgacca tccatgaaga caatagtata actgttgcag atagcggacg
    22741 tgggatgcct gtaggaatgc atgcttccgg aataccaaca gttgaagtga tttttactgt
    22801 gctgcatgct ggcggaaagt ttggtcaagg agggtacaaa acttctggtg gactgcacgg
    22861 tgtaggagcc agtgtggtaa atgccttatc gaaatggcta attgtaacga tcgttagaga
    22921 tggcgtagag tatcagcaga aatttaaaaa cggcggtaaa cctgacggta ctttgaaaaa
    22981 gataggaaaa acaaaaaaag caaacgggac gactgtccat tttttaccag atgatacaat
    23041 attcagtaca acgaaatttt catatgaaat tttagccgaa cggttaagag aatctgcctt
    23101 tcttctaaaa ggcgttaaaa tctctctttc tgatcttcgt ggagaagaac cagtcaagga
    23161 aatttttcat tacgaagaag gaatcaaaga atttgttgat tatctgaatg aagaaaaaga
    23221 tacattgaca ccagttgttt atttttcagg agaaaaagaa ggtatcgaag tagaggtcgc
    23281 ttatcaatac aatgacggtt attcagaaaa tgttttatct tttgtcaata acgttcggac
    23341 aaaagatggg ggaacccatg aagctggtat gaaagcggcg atgacgaaat cttataatga
    23401 atatgcgaga aaagttggtt tgctgaaaga acgggacaag aatctggaag gtagtgattt
    23461 cagagaaggg ttagctgctg tactttctat ccgtgtacct gaaaatcttc tgcaatttga
    23521 agggcaaaca aaagaaaaac ttggtacgcc agttgctcgt accgtagtag ataacgtcat
    23581 cagtgaacaa atgggctttt atttgcagga aaatagcgaa atgagccaga tgttggtccg
    23641 taaagcaatc aaagccagag aagcgagaga agcagcacgt aaagccagag aagaaagccg
    23701 aaatgggaag aagcgtaaaa aaggcgaatc tcttctttca ggaaaattga cacccgcaca
    23761 atcccgaaac cctaagaaaa atgaattgta tttagtcgaa ggggattctg ctggcggatc
    23821 tgctaaacaa ggaagagaca gaaagttcca agcaattcta cctttacgag gaaaagtcat
    23881 caataccgaa aaagctaaaa tgcaagatat cttgaaaaat gaagaaatca atacgatgat
    23941 ctatacgatt ggtgcaggag tcggtcctga gttttctatc gaagactgta attacgacaa
    24001 agtaatcatc atgaccgatg cggatacaga cggcgcccat attcaagtat tactgcttac
    24061 tttcttttat cgttatatga aaccgctgat tgaagctggg aaagtatata tcgcgttacc
    24121 gcctttatat aaagtatcca aaggacaagg aaagaagcaa gtcattgaat atgcgtggac
    24181 agatgacgaa ttggcagcaa tgattaaaaa agtcggcaaa ggatatatgc tgcagcgata
    24241 taaaggactt ggagagatga atgcggaaca attgtgggaa acaacgatgg atccgacttc
    24301 ccgtacgttg atccgcgtcc gtatcgatga tgctgcgcaa gctgaacgtc gagtaaccac
    24361 actgatgggt gacaaagtag aacctcgaag aaaatggatc gaaaatcacg tgcaatttac
    24421 gctcgaagaa gatggcagta tcttagataa aaaagaggat actgaaatct ctccttcagt
    24481 ttctaatgat ttattagatg aagaacgagc agataaaaat gaaaacaatc agttatttga
    24541 agtagaatag gaggtcacct gatggaaaat cgacaagaag tacaagaatt aactttagaa
    24601 gaagtgatgg gtgaccgctt cggacgttat tccaaatata ttatccaaga acgtgcactg
    24661 cctgatatcc gtgatggatt gaagccagtg caacgccgga tattatttgc catgaacaaa
    24721 gatggcaata cgtatgacaa aggattccgt aaatcagcaa aatctgtcgg aaatatcatg
    24781 gggaattatc atcctcatgg tgacatcagt atttatgaag cgatggtccg catgagccaa
    24841 gactggaagt tgcgggaagt actgatcgaa atgcacggga ataacgggag tatggacggt
    24901 gatccacctg ctgcgatgcg ttatactgaa gccagattat ccaagttaag tggagagatg
    24961 ctagcagata tcgagaaaga aacagtcgat ctcgtgtgga attttgatga tacagaaaaa
    25021 gaaccaacag tccttccagc tcgataccca aaccttctcg tcaatggatc gacaggtata
    25081 tctgctggat atgcaacaga aatcccgact cataacttag ctgaagtgat cgatggcaca
    25141 atctatatga tcgatcatcc acaagcgagc cttgaaaaat tgatggaata tattcctgga
    25201 cctgactttc caacaggagg gatcttgcaa ggaaaagatg aaatcaaaaa agcttatgaa
    25261 acaggacgag gaaaagttat tcttcgttcg aaaacaaaga tagaggcaat gaagggtaat
    25321 aaacagcaaa tcgtgatcac agaaatccca tatgaagtaa ataaagcgac cttggtgaaa
    25381 aaaatggatg agatccgttt gaataagaaa atcgatggaa tagctgaagt acgagatgaa
    25441 agtgatagaa caggtctgca aattgtcgtc gaattgaaaa aagacgccaa tgctcaagga
    25501 attttgaatt atttgttcaa aaacactgag ctacagatca attacaattt caatatggtc
    25561 gctatcgacc atatgactcc gcatcaagtt ggactgaaag acattttaag aagttatatc
    25621 gagcatcgaa agcaagtcat aacaaaacgt agtcaattcg atttagccaa agctcaaaaa
    25681 agacagcata tcgtagaagg attgatgaaa gctttatcga ttctggatga agtaatcgca
    25741 acgattcgtg aaagtaaaga caaaaaagac gccaaaaaga atttagtcga cgtctttcaa
    25801 tttacagaag aacaagcaga agcaattgtc acattgcaat tataccgttt gacgaacacg
    25861 gatatcacag agcttcaaaa ggaatccgaa agcttgattg cgcaaatcac agagttgaat
    25921 aaaatccttt caaatgacaa agagcttttc tctgttatga aaaaagaatt gcgagaagtg
    25981 aaaaaaaatt attcttccgc tcgtctaacg acaatcgaag acgaaatcga agaaatcaaa
    26041 attgatacgc aagtcttagt ggcacaagaa gacgtaattg tcagtgtgac gcgagaaggc
    26101 tatgtgaagc gaacaagcct tcgttcttat agtgcttcca aaccagaaga aatcgggatg
    26161 cgagaaggcg actacttgct atactcaggt gaattgagta cattggacca tgttcttctg
    26221 atcacaaata aagcaaatgt gatctaccgc cctgtccatg aactacccga tttgaaatgg
    26281 aaagatgctg gtgagcacat ttctcaaaca atctccaatc tgtctgtaga cgaatcgatt
    26341 ttagctgttt tcccatatca aaaaattcaa gcagagaaaa catttgtatt catcagcaag
    26401 aatggattga tcaagcaaac acgaatgaca gagttcgaac catggcgcac ctataagagc
    26461 cgtccgctca gctgtatgaa gctaaaggcg gaagaagatg aactgattgc tgtgtatctg
    26521 aaagaagatc agccaggctt agatgttttc ttagtaacgc atcaaggaat gggtttgcgc
    26581 tatcctttaa cagatgttcc cgttgttgga gcaaaggcag ctggagtgaa gtctatgaat
    26641 ttgaaagatg gagataggat tatcaacggg ctgcttgtgt tagcagaagg tgatacgccg
    26701 atcgtaatcg ttactcagcg aggagctgtt aaacgcatgc ttgctcaaga aatcagccaa
    26761 acttcacgtg cgaagcgggg agtgacggtc atgcgtgagc tgaaaaaaaa tccgcaccgt
    26821 attatctata tgtcggaagg aagatcaaaa gaaataacct tgatgaatca aaaagggcag
    26881 cagttaacga ttgacccaac tgatttccct ataggtgatc gaacatcgaa tggatcattt
    26941 gcgatggatg agaaaaaagg tggagaagtc atcgaagtta tcgatgctcc tgagattttc
    27001 atcgagtcgt aactgttgta gtattttaat gaagagacgc catctgtatt aatacaggtg
    27061 gcgtctcttt ttcattcatt tttaataaat ccttatacgt ttgcaaaaaa agatgaaagt
    27121 gttcacaatg cttaaatgtt ggtatgtcaa cgttgattta aatatatgta aataaaacat
    27181 aaaaattgtt aaataaaaaa caagaaaacc cttgcaaaga tagggtgctg tgttatacta
    27241 atcacgtaaa aactgttaca taaaacagaa atttaggagg tccatacaat ggagcaatgg
    27301 aatggattta aaggtaccaa atggaaagaa ggcgtagaca ctagagactt tattcaaaat
    27361 aactacactg aatatcgcgg tgatgacagc tttttggaag gcccaacaga agcaacagaa
    27421 aaattatggg atgaattgca aaagttaaat gatatccaat tcgagcgtaa cggtgtctat
    27481 gatatggaca cagaaatcgt atcaactatt acttcacacg atgcaggcta tttggataaa
    27541 gatttggaaa aaatcgtcgg tctacagaca gacaaacctt tgaaacaagc attcatgccg
    27601 ttcggcggaa tcaatatggc taacaatgcg ctaaaaagca atggctatac accaaacgat
    27661 gaattgacac atattttcac tgactggcgc aaaacacata atcaaggtgt atttgacgct
    27721 tatactgctg aaatgcgtgc tgctcgtaaa aacaaaatca ttacagggct tccagacgct
    27781 tacggtcgcg gacgtatcat cggtgactat cgtcgtgtgg cattgtatgg aatcgactat
    27841 ttgatggaac aaaagaaaaa agatcatgat atgactggtt atcatacaat gagtgaagaa
    27901 gtgattcgtt tacgtgaaga aatcactgaa caatatcgtg cattagcgca aatgaaagaa
    27961 atggctgcga aatatggcta tgatatttct aaaccagctg caaatgcaaa agaagctgtc
    28021 caatggctat acttcggata tttagcagca atcaaatcac aaaatggtgc tgcgatgtca
    28081 atcggacgta tctcagcatt ccttgatatc tatatccaac gtgatttgga taatggagta
    28141 atcacagaaa aagaagcaca agaattgatc gaccacttag taatgaaatt acgtatggtc
    28201 aaattcgcgc gtacacctga atataaccaa ttattctcag gattccctat ctgggctact
    28261 ttatccattg caggtatggg attagatggt cgttcattag ttacaaagaa tgatttccgt
    28321 ctgcttcata cattaacaaa tatgggacct tcaccagaac caaacttgac tgttttgtat
    28381 tctgaacacc ttccagaagg attcagaaca tatgctagca gaatcgctat cgaaagttct
    28441 tcgattcaat tcgaaaatga taatcagctt cgtgaacaat ggggttctga tgattgtgcg
    28501 attgcttgct gtgtatcagg aacagttgtc ggaaaagaca tgcaattctt tggcgctcgt
    28561 gctaacttgg ctaaaacact attatatgcc atcaatggcg gagtcgatga agtgactggc
    28621 gcccaagttg gaccagaata tcgtccaatc acatcagaaa tactagactt tgaagaagtg
    28681 aaacataact tcatgaatat gatggattgg ttagcagaac tatatgtcaa tacgttgaac
    28741 atcattcatt atatgcatga taaatattct tatgaagcag ctcagctggc tttgatggat
    28801 tctgacttgc aacgcacatt tgctacaggt attgctggga tctctcatgc ggcagacagc
    28861 ttgtcagcga tcaaatatgc aaaagtaaaa gcaatccgta atgaaaaagg aatagcagtt
    28921 gattttgaag tggatggcga cttccctaaa tacggaaatg atgatgaccg tgcagatgaa
    28981 ttagtttcat ggatcttgga atacttcatg ggacaaatca aacgtcataa aacttaccgc
    29041 aactcaaaac caacaacttc attattgacg attacttcaa atgttgttta cggtaaaaat
    29101 acaggaaata ctcctgatgg ccgtgaagct ggaaaaccac ttgcgccagg agcaaatcca
    29161 agttacaatg cggaacaaag tgggttatta gctagcctga actcaacagc taaaatgaat
    29221 tacggctgtg cattggatgg aatttcaaat acccaaacaa tcaatccttc aggtcttgga
    29281 aaagaacaag atacaagaat cgaaaacttg cgtaatgtct tagatggata cttctcaaaa
    29341 ggagcttatc atttgaatgt caatgtattc agtaatgatt tattgcgtga tgcaatggaa
    29401 aatccaatga aatatccgaa cttgacgatc cgggtatctg gttatgcagt taaattccgt
    29461 gacttgacaa gagaacaaca attagatgtc ttgatgagaa cttcacacga cagaatgtaa
    29521 aaagttcgtg aaaaaagttt ttcgacttag cgtcgaaagg acgttgaata gccatcgcat
    29581 caaaatcgaa taatcagtat gcttatttga aagaaacgat ttacaggggc aggacttgta
    29641 tgttccgtcc ctgtaaattt tacacataac gaagcggcaa ttgcgaaaaa ttttgttcag
    29701 aaagcttcgc ttcgacagct aacttgtact taaaatgaaa tcaaagaaaa gatgtgaact
    29761 taaatggaag aaaaaacaat cggctatgtt cattctatcg aaactttcgg ttcagtagac
    29821 ggacctggac tgcgctttgt cgtgtttatg caaggatgcc gcatgcgttg ccaattttgt
    29881 cacaatccag atacttggaa tattggcgga gggaaagaat acacagcaga tgaactttta
    29941 gataaagcag aacgcttcag accttactgg ggagataaag gtggtattac tgttagtggt
    30001 ggagaacctc tattgcagat cgattttttg atcgaactat ttaaaaaagc aaaagagcgg
    30061 aagatgcata ctacattgga tacatgcgga aaacctttta cgtatgaaga acctttcttt
    30121 tcacgttttc aagaattgat gaagtataca gatttactgt tatttgacat caaacacatc
    30181 gataatgaag agcataaaaa actaacgcat tgggacaatg aaaatatttt agaaatggct
    30241 caatatttgt caaaaatcaa caaaccggtt tggattcgcc atgtactagt tcctgagcga
    30301 agtgattatg atgaatattt gatccgacta gacaacttta ttcaaacctt gtcaaatgta
    30361 gacagagtag agattcttcc ttatcatacg atgggaaaat acaagtggga aacacttggt
    30421 ttgaaatatc cattagaagg gatcgaacca cctacaaaag aacgtgtcga aaatgctaaa
    30481 cgattgcttc atacagaaga gtatacaaaa tatttaacgc gataggtcgc atcggaaaga
    30541 agataagtat tgcttctttc cgtttttttg ctattattaa ataggagaaa tcacaaagga
    30601 gcgaatgaca tgtcaaaaat tttagttttt ggtcaccaaa atccagatac agatgcaatc
    30661 ggggcagcca ttgcgttttc acacttgcaa aatgaattag gtaaagaaac agaagcagtt
    30721 gctctaggta cacctaatga agaaacacaa tacgcattag accactttca gctatctgca
    30781 ccacgagtag tagagtcagc taaagcagaa aatgcagatg tcatgcttgt ggatcataat
    30841 gaattccaac aaagtatttc agatatcagc gaagtaaata ttttagctgt agtagaccac
    30901 catcgtattg caaactttga aacagctaat ccattgtact atcgtgcaga accagttgga
    30961 tgtacaagta cgattatttt aaaactatat aaagaaaacc aagtaacaat tcctaaagaa
    31021 atcgcaggaa tcatgctctc agctatcatt tcagatacat tgttgttcaa atcaccaaca
    31081 tgtacgcctg aagatgaagc agctgcaaaa gaacttgcag caatcgctga agtcgatcta
    31141 aacacttatg gacttgaaat gttgaaagca ggaacaaatc taagtgataa atcagctgcg
    31201 gttcttcttg atttggatgc aaaaagtttc ccaatgggca gttccaacgt tcgtatcgct
    31261 caagtcaata cagtggacct taatgaagtg atggatcgac aagcagagct agaagcagca
    31321 atggaagatg aaatgaaagc aaatggctac gatctgttcg tattagttgt cacaaacatc
    31381 ttagacagtg attcagaatt acttgttgct ggtaaaggaa aagaaaaagt agaagaagct
    31441 ttccaaacaa ctttgaccaa caaccgtgct ttgcttaaag gtgtcgtttc acgtaaaaaa
    31501 caagtcgtac ctcaattgac ggctgtattt agctaatcaa atggtagtat gaagcattca
    31561 aaatgaatgg ataagcgatt gatataaaaa gaaacataga acatactaaa ggccgaagaa
    31621 atggaggaga ataacagtca attatgactc ttgttcttcc tcctttttac gtaaaagcct
    31681 tttaagtaaa ataaaacgga tagaaaagag aacatatatg aactattatt caaaaaataa
    31741 ggaaaacttt tatcaaaaac tgaccgatga tccgcttttt tcttcattga ccgattatct
    31801 atatgaacat cgagaacaag aaacgattct aagagaattg aaaaaagaat tttcgcaaaa
    31861 taaattttcc cattttcttg atttattgat cgatgcagga ttaatcaaga gggaagaacg
    31921 aagatatcat ttgaactttc caatatttga tcctaaagat tacttgcaac aagccacttc
    31981 ggctgctgaa accatagctg aacaattgaa gagactctct gttgatgaac aaaaattagc
    32041 gatgggagaa gttatttggg cgtattgttt cgaagatgaa cggaaagaag cgtattttta
    32101 tggagtaaga aacagccgag agacagaatt gctgaggaca actgcaggaa atgagaagta
    32161 ccgttttatc acactttcct ctatagaaca cttcccgctt actttggcta attacttttt
    32221 tgtacagaag aatcagcttc ctgtaacgaa ggcattcaaa gaattagccg aacttattgg
    32281 agacgtaaat gaatcgtact tttttgatca aatagaagtt atcgttgaca ggatacgtaa
    32341 gaataaatat aaaaatcgcc ggcctagtat tttccatcag tctctacttg tgacgaatac
    32401 tataaaggaa gaagaatcat tcacattaga attacctatt gtcgaaaaaa acaattttga
    32461 gattgaatta ccaacattgg atccttcatt gacaatggag gaaatagcct ttcttaaacg
    32521 acaaatcttt tctgagctga gcaagaaatt tataccgcat gcattttctt atatcaaaga
    32581 atatggaaca gttttagtga gtaaaaccta agagagcagt aagcaaaaat tatactgact
    32641 cttgcaggag gagcctttct cttcctttat ggttaaaaga attatttgag tattttgcct
    32701 atctaccacc tattagattc actacatggt ctggaaacaa aggaatgcaa aaaaatggtg
    32761 agaaatgtgg aatgacaaag gaagtagtga taagaaaagt tagattttta aataatcaat
    32821 attatgattc agtcaagtat ggcattttat gggaagaact agctgattag attaggaagt
    32881 ccatttgata atccagaatc actaagaaac gaatggcgag tcattaataa tttatccaat
    32941 cttgggttat tcttttagat tgaagtgttt aaaactaaaa aagcatttat gaagtcgtaa
    33001 tagtcaggct tcaaaatgct ttttattttg tttgtttttt ctttgccaca taaatacacc
    33061 tagactgacg atgaagagca tatccaacag atttcctgcg acttggatcg tcgaagggtt
    33121 ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa aacaggtgac
    33181 aggaactaat cgaaagaaga gcggacattt caattggcta aaaatgagtg aaaaaataaa
    33241 ataagtaaaa atggataaaa tcacgactaa accattcatg ataaagaaga aaagcgtatt
    33301 gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg caatcaatac
    33361 gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa aaataggcac cgaaataaac
    33421 agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt cttcggcttc
    33481 tgtgatatca gaatgatcca aaagcaacca gcctaatgca ctagaaggcg catgtgaggt
    33541 cttaaggtat gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa aagagcctaa
    33601 atgtaaaaaa ttagtggttc tacgttgaat ttccataata tctccaaat
//
LOCUS       DAZKAO010000017        33259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000017 DAZKAO010000000
VERSION     DAZKAO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.017"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1053
                     /locus_tag="ACG8H8_000642"
     CDS             46..1053
                     /locus_tag="ACG8H8_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8985741.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            1078..2262
                     /locus_tag="ACG8H8_000643"
     CDS             1078..2262
                     /locus_tag="ACG8H8_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP8985742.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2699..4282)
                     /gene="abc-f"
                     /locus_tag="ACG8H8_000644"
     CDS             complement(2699..4282)
                     /gene="abc-f"
                     /locus_tag="ACG8H8_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP8985743.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(4387..4515)
                     /locus_tag="ACG8H8_000645"
     CDS             complement(4387..4515)
                     /locus_tag="ACG8H8_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985744.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(4860..5003)
                     /locus_tag="ACG8H8_000646"
     CDS             complement(4860..5003)
                     /locus_tag="ACG8H8_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP8985745.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(5226..5705)
                     /locus_tag="ACG8H8_000647"
     CDS             complement(5226..5705)
                     /locus_tag="ACG8H8_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8985746.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(5778..6062)
                     /locus_tag="ACG8H8_000648"
     CDS             complement(5778..6062)
                     /locus_tag="ACG8H8_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985747.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(6140..6334)
                     /gene="ssrS"
                     /locus_tag="ACG8H8_000649"
     ncRNA           complement(6140..6334)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8H8_000649"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6406..7692)
                     /locus_tag="ACG8H8_000650"
     CDS             complement(6406..7692)
                     /locus_tag="ACG8H8_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP8985748.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7830..8501
                     /locus_tag="ACG8H8_000651"
     CDS             7830..8501
                     /locus_tag="ACG8H8_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP8985749.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            8467..8955
                     /locus_tag="ACG8H8_000652"
     CDS             8467..8955
                     /locus_tag="ACG8H8_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP8985750.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            8968..9915
                     /gene="prmA"
                     /locus_tag="ACG8H8_000653"
     CDS             8968..9915
                     /gene="prmA"
                     /locus_tag="ACG8H8_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP8985751.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            9916..10680
                     /locus_tag="ACG8H8_000654"
     CDS             9916..10680
                     /locus_tag="ACG8H8_000654"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP8985752.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            10913..13126
                     /locus_tag="ACG8H8_000655"
     CDS             10913..13126
                     /locus_tag="ACG8H8_000655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP8985753.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            13137..13583
                     /gene="dtd"
                     /locus_tag="ACG8H8_000656"
     CDS             13137..13583
                     /gene="dtd"
                     /locus_tag="ACG8H8_000656"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP8985754.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            13754..15013
                     /locus_tag="ACG8H8_000657"
     CDS             13754..15013
                     /locus_tag="ACG8H8_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP8985755.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(15102..16049)
                     /locus_tag="ACG8H8_000658"
     CDS             complement(15102..16049)
                     /locus_tag="ACG8H8_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8985756.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            16443..17735
                     /gene="hisS"
                     /locus_tag="ACG8H8_000659"
     CDS             16443..17735
                     /gene="hisS"
                     /locus_tag="ACG8H8_000659"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP8985757.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            17750..19519
                     /gene="aspS"
                     /locus_tag="ACG8H8_000660"
     CDS             17750..19519
                     /gene="aspS"
                     /locus_tag="ACG8H8_000660"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP8985758.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            19628..20503
                     /locus_tag="ACG8H8_000661"
     CDS             19628..20503
                     /locus_tag="ACG8H8_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8985759.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            20769..22652
                     /locus_tag="ACG8H8_000662"
     CDS             20769..22652
                     /locus_tag="ACG8H8_000662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP8985760.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(22727..23011)
                     /locus_tag="ACG8H8_000663"
     CDS             complement(22727..23011)
                     /locus_tag="ACG8H8_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP8985761.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(23001..23735)
                     /locus_tag="ACG8H8_000664"
     CDS             complement(23001..23735)
                     /locus_tag="ACG8H8_000664"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP8985762.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            23864..24487
                     /locus_tag="ACG8H8_000665"
     CDS             23864..24487
                     /locus_tag="ACG8H8_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP8985763.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            24679..25800
                     /locus_tag="ACG8H8_000666"
     CDS             24679..25800
                     /locus_tag="ACG8H8_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP8985764.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            25807..28935
                     /locus_tag="ACG8H8_000667"
     CDS             25807..28935
                     /locus_tag="ACG8H8_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8985765.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            28954..32148
                     /locus_tag="ACG8H8_000668"
     CDS             28954..32148
                     /locus_tag="ACG8H8_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP8985766.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(32294..33001)
                     /locus_tag="ACG8H8_000669"
     CDS             complement(32294..33001)
                     /locus_tag="ACG8H8_000669"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8985767.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10872 a         5990 c         7074 g         9323 t
ORIGIN      
        1 acaggtgaaa aaattgttat aatatatcta gaaatgaggt gcaccatgtt accagaaaaa
       61 atggaacaag ggtttaatca aaacctggaa gctattcagc ttttacagaa tgcattggga
      121 acttcctttt tagaagccta tgtcgaaaat gcagaaaatt taatagatga ttatcaagta
      181 cgtgtggtgg atggagtgcc gacaaaagag acaacgcaac ggatcacagc tctttatgaa
      241 gagttgaaga aactttcttt cgagccagaa gaatggcgca gactgtctca attactttta
      301 ttgaagggca gccagacaga acatttgcag gcgaaccatc aactcactcc tgatagtatc
      361 ggttttttat ttgtttttct tattgaacaa ctatacacaa ataaaaaagc accggttaag
      421 attctggata ttgcggcagg aatgggcaac ttgttattaa cagttctttt aaacctatcg
      481 aatgcggggt accaaacaga aggaattggt gtggatattg atgatacatt gttggctgtc
      541 gcagcttcta caagtgattt gacacaagca aatgttcaat actttcatca agacgggttg
      601 caggaattat taatagatcc tgtcgatttt gcaatcagcg atttgccaat cggttattat
      661 ccgaatgacg aaaaagcaaa agagttttta acaagtaccg aagaaggcca tagttatgca
      721 catcatttat tgctagagca atcaatgaaa tatgtgaagc cggatggttt tggtttgttt
      781 ttgatgccga gtggattttt agaaacggat caaagtgaag aaatcaaaaa atggtttaaa
      841 gaagaaggtt atcttcaagg aatgattcaa ttgccagatg aattgttccg taataaacaa
      901 tcgcaaaaaa gtattttgat cttacagaaa aaaggaccac aagcacaaca agtaaaagaa
      961 gtcttgcttg tgaaacttgc ttcattaaaa gaaccagaga aggtgacgga atttttcaac
     1021 gagtttaaaa actggaaatc atctgctctg taatcattca acgagaagga gagtcatatg
     1081 tctaaaacaa ttgcaatcaa tgctgggagt tcaagtttga aatggcaatt atatcaaatg
     1141 ccagaagaaa ccgtagttgc gaaagggatc gtcgaacgta tcggtttaca agattctatt
     1201 tttaccatca agtacggcga taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac
     1261 gaagtagcag taaaaatgct gttagatcag ttgattgaac tgcaaatttt aagctcttat
     1321 gatgaaatca ccggagtagg gcaccgtgtt gttcacggag gagaaactta tggggattca
     1381 gtagtgatcg atgagaaagt aatggaccgc attgctgaat tagcagaatt tgctcctttg
     1441 cataatcctg caaacttaat ggggatcaaa gcgtttaaaa agattttacc tgacattttg
     1501 agtgtggctg tattcgatac atctttccac tcaacaatgc caaagcaaaa ttatttatat
     1561 agcttaccgg tgtcttatta tgaagatttc aaagtaagaa aatatggctt ccatggcaca
     1621 agtcatcgtt atgtgtcgga acgtgcggca aaattattga acaaaccgat cgaagaacta
     1681 aaaatcatca cttgccactt aggaaatggt gtatcgatca cagcagtcga tggaggtaaa
     1741 tccatggata cttctatggg attcacacca ttagctgggg tgacaatggg tacacgttca
     1801 ggtgatatcg atccagctgt tctcccatat ttaatggaaa aattagagat tgatattgat
     1861 gaaatgatca atgttttgaa caagaaatct ggtctgctag gcctaacagg attgtctagt
     1921 gatatgcgtg atcttgaaaa gaactatgaa aaagaacata ttcgtttagc ttatgacatt
     1981 ttcgtggacc gcatccgaaa atacatcggc ggttatgtga caacgatgaa tggtgttgat
     2041 gcaattgtct ttactgctgg tatcggcgag aatgacggac atgtacgtag tgaaatcatc
     2101 aaaggtatgt cttggtttgg ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa
     2161 gaagtagata tctctacaaa agaatcaaaa gtaagagtat tagtgatccc aacagatgag
     2221 gaactaatga ttgcccgtga tgtggagcgt ctgcgcggct aagccaaagc attcgttagg
     2281 ataaaagatg aaacgcatgc aagagggaat tcttgcatgt gttttttttg cggagaagac
     2341 cgcaaaatgg taatttaact tcaatttccc ttctttttta caggatcttc ttagggaatt
     2401 ttgcttggaa aagtggtcaa aagggaaaag actggcattt tatttattga ttttcaggaa
     2461 aatcccaata ttttattttc attcatcttt ataagggaca ttacatatgt aaaaagaaaa
     2521 aacattttta gatcagcctc gtgggctgtc gactttattc ttcgctttat gtttagctag
     2581 aaaaccgatc cgaaacattc attgatcgaa taataaaaac ttgattttcc acctgtactt
     2641 tgcctgaatc gtgaaagcac aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct
     2701 agtcaatcag ttggccgagc ggctggcggc tgatctgctc ctgatcaata acgagtgctt
     2761 cttggaagtg ttgctgcaaa aagtagcggt catggctgac tgctaggagc gttccttgat
     2821 agtcttgcag catttcttca atttcttctc gagcatagct gtcgaggtga ttggttggtt
     2881 catccaaaat caggacgttg attttttcta aaaagagttt cagcaacgcc aaacggattt
     2941 tttcaccacc ggataaatct ttgatccgtt tacccacatc gctttgataa aagccacttt
     3001 tggctaattg ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg taagccagca
     3061 aacggctatc gggatcggca aactgcagct gctgcggcaa ataaccgatt tgcaagctac
     3121 tacctaattt gatggtgcca ccgtaaggct tttcctcacc gagtaataat ttgagtaagg
     3181 ttgattttcc tgcaccattg tcgccgatga tggctaaatg atctccacga tagactgcaa
     3241 aactgctgtc tttgaatagt acttgatcag cgtactgctg gtgcaagttt tgaatgatga
     3301 aaacttcttt gccagatttt ccaccattag acaagctttg cagccgattt ttcgggggag
     3361 gtggcggttt gacgagtgtc agtttcgcca ggcggcgttc cagttctttg gcttttttga
     3421 agaaggactc attgtctcct tcatgtgccc actgacgaaa acgacgaatc atgactttca
     3481 accgctggac ttctttttgc tgcagttcgt aattttttgt cagctcagcc aagcgagctt
     3541 ctttcaagac tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca ccttcttcca
     3601 gttcccatgt ttttgtggtg acttgatcga gaaattgccg atcatgagaa acgaccagaa
     3661 ccgatttttt tgtattctgt aaatagtttt ccaaccagcg aatactgtta agatctaaat
     3721 gattggtcgg ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag
     3781 cgactttaac tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa
     3841 tccctaagcc ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt
     3901 caaagcgatg ctgcagctca ccgtaacggg ccagacactt ctctaagttt gtatctggtt
     3961 ctgtcatcaa tgcttcgtag cgactcattt gctgtttgag gtcctgtatc tcagcggcag
     4021 aatggagcaa gtagtggttg acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc
     4081 cgatctttaa gccttttttg cgactgataa caccgctgtc gacaccttct ctgccggtca
     4141 agatttgtaa aaaggttgat ttgccagttc cgttgatacc gactaagaca attttttcac
     4201 cagcttcaat ttgacaggat aaatcctcaa ataatggatt cccgccgatt gtttttttaa
     4261 tagaaattgc ttgtatgatc atgatctctc tccttgagac cactcaaaaa ggccatggtt
     4321 atccatggcc tgtgttattt aaactcagat tatcatgaga aaaatacctt gagatggcca
     4381 ttgattttac gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag caactgtaaa
     4441 accaattgca tggctaaatg cgatatggta tcgttggtaa ttttcattac ttgttatttt
     4501 agtcatgcgc atcatagtat tttccctcct tcgtgtagtt gtcttttcca gtgtacctga
     4561 aagtgtcaga aagttcaact agcgctgaca tttacttaat atttatcgat gtgtaattct
     4621 gtcagagagc tttttttatc tgactgaggg taatgtctcg tggaccacat cattgtattc
     4681 tgagctaaac cagcgttcag gcgaaatcat atggtgtttg ataaaaaact ttgtctacat
     4741 gacttccttt tgccatttgc ttaataccgt tgcattaaac agctcttctc tttggaaatc
     4801 tcaccaacat cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt agcctttcat
     4861 caattcttga atgatttacc gaatgtagca cttgccttct cgcggtaccc gaacccgaaa
     4921 taaaatttta gtttggcgat aaaatcggta tccgccatcg atacttcata ttttttcgta
     4981 tcagattcat cggaaacttc cacattgacg aaccaataag ctttggcatg cttaggtcgt
     5041 tttaaaccac accagggcga ctaatcgccc tggtgttcac ttttttttat tttacttgaa
     5101 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
     5161 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
     5221 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
     5281 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
     5341 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
     5401 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
     5461 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
     5521 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
     5581 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
     5641 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
     5701 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
     5761 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
     5821 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
     5881 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagctcc gtaaaattgt
     5941 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
     6001 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
     6061 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
     6121 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
     6181 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
     6241 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
     6301 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
     6361 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
     6421 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
     6481 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
     6541 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
     6601 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
     6661 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
     6721 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
     6781 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
     6841 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
     6901 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
     6961 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
     7021 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
     7081 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
     7141 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
     7201 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
     7261 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
     7321 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
     7381 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
     7441 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
     7501 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
     7561 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
     7621 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
     7681 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
     7741 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
     7801 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
     7861 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
     7921 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
     7981 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
     8041 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
     8101 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
     8161 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
     8221 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
     8281 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
     8341 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
     8401 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
     8461 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
     8521 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
     8581 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
     8641 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
     8701 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
     8761 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
     8821 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
     8881 cagcaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
     8941 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
     9001 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
     9061 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
     9121 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
     9181 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
     9241 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
     9301 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
     9361 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
     9421 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
     9481 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
     9541 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
     9601 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
     9661 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
     9721 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
     9781 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
     9841 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
     9901 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
     9961 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    10021 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt
    10081 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    10141 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    10201 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    10261 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    10321 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    10381 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    10441 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    10501 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    10561 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    10621 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    10681 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    10741 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    10801 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    10861 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    10921 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    10981 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    11041 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    11101 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    11161 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    11221 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    11281 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    11341 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    11401 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    11461 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    11521 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    11581 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    11641 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    11701 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    11761 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    11821 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    11881 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    11941 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    12001 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    12061 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    12121 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    12181 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    12241 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    12301 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    12361 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    12421 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    12481 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    12541 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    12601 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    12661 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    12721 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    12781 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    12841 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    12901 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    12961 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    13021 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    13081 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    13141 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    13201 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    13261 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    13321 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    13381 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    13441 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    13501 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    13561 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    13621 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    13681 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    13741 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    13801 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    13861 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    13921 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    13981 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    14041 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    14101 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    14161 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    14221 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    14281 ttctccgatt gatcacataa aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca
    14341 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    14401 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    14461 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    14521 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    14581 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    14641 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    14701 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    14761 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    14821 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    14881 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    14941 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    15001 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    15061 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    15121 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    15181 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    15241 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    15301 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    15361 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    15421 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    15481 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    15541 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    15601 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    15661 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    15721 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    15781 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    15841 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    15901 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    15961 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    16021 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    16081 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    16141 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    16201 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    16261 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    16321 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    16381 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    16441 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    16501 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    16561 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    16621 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    16681 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    16741 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    16801 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    16861 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    16921 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    16981 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    17041 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    17101 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    17161 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    17221 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    17281 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    17341 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    17401 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    17461 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    17521 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    17581 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    17641 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    17701 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    17761 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    17821 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    17881 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    17941 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    18001 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    18061 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    18121 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    18181 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    18241 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    18301 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    18361 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    18421 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    18481 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    18541 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    18601 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    18661 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    18721 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    18781 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    18841 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    18901 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    18961 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    19021 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    19081 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    19141 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaact
    19201 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    19261 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    19321 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    19381 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    19441 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    19501 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    19561 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    19621 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    19681 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    19741 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    19801 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    19861 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    19921 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    19981 ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg
    20041 cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc
    20101 gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt
    20161 tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga
    20221 tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa
    20281 agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga
    20341 aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact
    20401 gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga
    20461 aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa
    20521 ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta
    20581 gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat
    20641 gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct
    20701 acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg
    20761 aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg
    20821 ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga
    20881 aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc
    20941 tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa
    21001 gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca
    21061 ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt
    21121 atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg
    21181 agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc
    21241 cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg
    21301 tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg
    21361 atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg
    21421 cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa
    21481 atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt
    21541 tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg
    21601 tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc
    21661 cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta
    21721 tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag
    21781 tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc
    21841 tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga
    21901 tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg
    21961 ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa
    22021 aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta
    22081 gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg
    22141 cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc
    22201 tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca
    22261 gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgatcg
    22321 gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg
    22381 gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg
    22441 tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt
    22501 acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg
    22561 aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta
    22621 cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca
    22681 tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag
    22741 gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt
    22801 tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca
    22861 tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt
    22921 cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga
    22981 ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat
    23041 tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct
    23101 tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat
    23161 tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga
    23221 tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg
    23281 atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg
    23341 ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa
    23401 gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt
    23461 tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt
    23521 tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt
    23581 cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc
    23641 acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg
    23701 tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat
    23761 agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat
    23821 tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat
    23881 gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa
    23941 agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga
    24001 cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga
    24061 actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa
    24121 aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa
    24181 tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat
    24241 ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt
    24301 gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga
    24361 cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca
    24421 agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa
    24481 aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc
    24541 agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat
    24601 atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac
    24661 attttttgag gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa
    24721 ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc
    24781 gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc
    24841 ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa
    24901 aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt
    24961 acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa
    25021 ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg
    25081 agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata
    25141 gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt
    25201 gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg
    25261 atccctggtg aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa
    25321 gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct
    25381 gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat
    25441 tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg
    25501 ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact
    25561 gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg
    25621 gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa
    25681 atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta
    25741 actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga
    25801 tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc
    25861 gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct
    25921 ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga
    25981 cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg
    26041 actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt
    26101 ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt
    26161 tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg
    26221 atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa
    26281 ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat
    26341 ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa
    26401 cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt
    26461 ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac
    26521 catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag
    26581 gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat
    26641 gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata
    26701 gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc
    26761 cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa
    26821 acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac
    26881 cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg
    26941 atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt
    27001 tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag
    27061 gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt
    27121 cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa
    27181 gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa
    27241 ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc
    27301 caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt
    27361 cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag
    27421 gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact
    27481 cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat
    27541 ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa
    27601 caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta
    27661 gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag aatagagctg
    27721 gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag
    27781 cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa
    27841 caattgcaag atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag
    27901 ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa
    27961 gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat
    28021 gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt
    28081 tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa
    28141 cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta
    28201 gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca acagttagaa
    28261 gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa
    28321 attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa
    28381 ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag
    28441 aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca
    28501 aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt
    28561 ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg
    28621 tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca
    28681 ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt
    28741 attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta
    28801 gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa
    28861 gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc
    28921 tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc
    28981 attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa
    29041 gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat
    29101 ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt
    29161 aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa
    29221 aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa
    29281 ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa
    29341 taccaagtag aaacgattga gactaatcag tatcacccag agctttctat tttggcactt
    29401 tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat
    29461 gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt
    29521 cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga
    29581 agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat
    29641 aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag
    29701 actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca attttccgat
    29761 caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca
    29821 ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt
    29881 tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga
    29941 ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt
    30001 atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta
    30061 gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct
    30121 cgagtcgatt tcgtctatgg cgaagtcatc tattcgactg acccgaagca tgaggtctcg
    30181 acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta
    30241 ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg
    30301 gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc
    30361 agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc
    30421 gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag
    30481 caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa
    30541 aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa
    30601 caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga
    30661 ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg
    30721 atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat
    30781 gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat
    30841 cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc
    30901 tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg
    30961 agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa
    31021 gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga
    31081 gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat
    31141 tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca
    31201 ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat
    31261 ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca
    31321 aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg
    31381 cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct
    31441 gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa
    31501 atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg
    31561 acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt
    31621 tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga
    31681 gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag
    31741 ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg
    31801 gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga
    31861 actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct
    31921 gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa
    31981 gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa
    32041 aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact
    32101 gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa
    32161 agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa
    32221 aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt
    32281 ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag
    32341 aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa
    32401 gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt
    32461 ataaggtctc ttctggaagc atatgaaatt ctttctcagc tagctgaaaa atctcttttt
    32521 ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt
    32581 tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc
    32641 ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc
    32701 agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa
    32761 tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta
    32821 gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga
    32881 tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc
    32941 gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca
    33001 aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca
    33061 gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct
    33121 tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat
    33181 aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc
    33241 tctgtggaat tttcgaaag
//
LOCUS       DAZKAO010000018        32377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000018 DAZKAO010000000
VERSION     DAZKAO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.018"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..3383
                     /gene="fss3"
                     /locus_tag="ACG8H8_000670"
     CDS             156..3383
                     /gene="fss3"
                     /locus_tag="ACG8H8_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP8985768.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3495..3809
                     /locus_tag="ACG8H8_000671"
     CDS             3495..3809
                     /locus_tag="ACG8H8_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8985769.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3822..4196
                     /locus_tag="ACG8H8_000672"
     CDS             3822..4196
                     /locus_tag="ACG8H8_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8985770.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4206..4550
                     /locus_tag="ACG8H8_000673"
     CDS             4206..4550
                     /locus_tag="ACG8H8_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP8985771.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4619..5635
                     /locus_tag="ACG8H8_000674"
     CDS             4619..5635
                     /locus_tag="ACG8H8_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP8985772.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6229..8103
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000675"
     CDS             6229..8103
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000675"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP8985773.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8205..8585
                     /locus_tag="ACG8H8_000676"
                     /pseudo
     CDS             8205..8585
                     /locus_tag="ACG8H8_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8630..9772
                     /gene="dcm"
                     /locus_tag="ACG8H8_000677"
     CDS             8630..9772
                     /gene="dcm"
                     /locus_tag="ACG8H8_000677"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFP8985774.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9762..10051
                     /locus_tag="ACG8H8_000678"
                     /pseudo
     CDS             9762..10051
                     /locus_tag="ACG8H8_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10307..11491
                     /gene="mobT"
                     /locus_tag="ACG8H8_000679"
     CDS             10307..11491
                     /gene="mobT"
                     /locus_tag="ACG8H8_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP8985775.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11488..11601
                     /locus_tag="ACG8H8_000680"
     CDS             11488..11601
                     /locus_tag="ACG8H8_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP8985776.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            12371..14281
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000681"
     CDS             12371..14281
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000681"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP8985777.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            14385..14609
                     /locus_tag="ACG8H8_000682"
     CDS             14385..14609
                     /locus_tag="ACG8H8_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985778.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            14622..15125
                     /locus_tag="ACG8H8_000683"
     CDS             14622..15125
                     /locus_tag="ACG8H8_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP8985779.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            15252..16289
                     /locus_tag="ACG8H8_000684"
     CDS             15252..16289
                     /locus_tag="ACG8H8_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFP8985780.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            16304..18001
                     /locus_tag="ACG8H8_000685"
     CDS             16304..18001
                     /locus_tag="ACG8H8_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFP8985781.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            17994..18455
                     /locus_tag="ACG8H8_000686"
     CDS             17994..18455
                     /locus_tag="ACG8H8_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP8985782.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            18468..19328
                     /locus_tag="ACG8H8_000687"
     CDS             18468..19328
                     /locus_tag="ACG8H8_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985783.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            19366..19755
                     /locus_tag="ACG8H8_000688"
     CDS             19366..19755
                     /locus_tag="ACG8H8_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8985784.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            19742..22189
                     /locus_tag="ACG8H8_000689"
     CDS             19742..22189
                     /locus_tag="ACG8H8_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8985785.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            22194..24302
                     /locus_tag="ACG8H8_000690"
     CDS             22194..24302
                     /locus_tag="ACG8H8_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP8985786.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            24299..25303
                     /locus_tag="ACG8H8_000691"
     CDS             24299..25303
                     /locus_tag="ACG8H8_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP8985787.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            25322..26233
                     /locus_tag="ACG8H8_000692"
     CDS             25322..26233
                     /locus_tag="ACG8H8_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8985788.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            26420..26707
                     /locus_tag="ACG8H8_000693"
                     /pseudo
     CDS             26420..26707
                     /locus_tag="ACG8H8_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26895..27110)
                     /locus_tag="ACG8H8_000694"
     CDS             complement(26895..27110)
                     /locus_tag="ACG8H8_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985789.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(27369..27578)
                     /locus_tag="ACG8H8_000695"
                     /pseudo
     CDS             complement(27369..27578)
                     /locus_tag="ACG8H8_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(27881..29320)
                     /locus_tag="ACG8H8_000696"
                     /pseudo
     CDS             complement(27881..29320)
                     /locus_tag="ACG8H8_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(29868..30539)
                     /locus_tag="ACG8H8_000697"
     CDS             complement(29868..30539)
                     /locus_tag="ACG8H8_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP8985790.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(30888..32339)
                     /locus_tag="ACG8H8_000698"
     CDS             complement(30888..32339)
                     /locus_tag="ACG8H8_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8985791.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        10821 a         5447 c         6627 g         9482 t
ORIGIN      
        1 ggcagtatat ttcgtatttt atattaaatt tttaggctca ttcattctca aatccattct
       61 atttagtcaa taaatatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt
      121 atttttttgt gcctaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat
      181 tatgtgtatt aactattttg agtttggctg ttcaatcaat tgtaggagca attgctccta
      241 cgattgcttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag
      301 cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg
      361 ttccgcatta tgcagtgtac aacggcgtta aacaagatat tttctgtgtt gaaccaggag
      421 tcccgattta caatgagttc actcctggat atgagaagaa cccgttacca gatatgtccg
      481 ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata
      541 ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac
      601 gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaatcaat caagccattg
      661 ccgattatca gaagaaacca agcttccata atagtacggc taaaacagta ttagggcaat
      721 cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa
      781 ataccgcaaa cattgattat cgtgtgaatg gcaatcagtt gataatcagc ccaaatgcca
      841 attcaaaaga aagtggtgtg ttgacgctta agaaatctgc tggtaccgga acacctgtcg
      901 cctataaaaa ggctggccaa cagactttaa tggccggggc gatcgataag ccgaacactt
      961 atacggtcaa aattgatgtg gaaatggagg gttctttgaa gattaaaaaa gttgataaag
     1021 aatcgggtga tattatacca ggaacggttt tccatttaga ctttggaaaa actttacccg
     1081 caaaagacgt gaccaccgat aaagaaggca ttgcgacctt agatgagatt cctcatggta
     1141 caaaggtaac cattactgaa aaatcggtgc cggcgcctta tacgattgat accacaccca
     1201 tgactgccac aattaaagca ggtgaaacca tttcagtgac ttcaaaaaat acgcgagaaa
     1261 aaggtcaaat tattctggat aaaaccggaa ttgaaacagg gagtgatctt tggaacgata
     1321 attactcttt agcgggcaat acgtttgcca ttcgcaagga tagcccaact ggtgaaatcg
     1381 tccaagaaat gaccacggat gaaaaaggtc atgcagaaac accaaaagag attgccaatg
     1441 cgttggaatt gggaacctac tatgtgactg aaaccaaggc tagtcatggc tttgtgaata
     1501 ctttcaaacc agtaaaagtc gaattgaatt atgtcaatca aacagtggct cttgttacca
     1561 gtaacgtaaa agggcaaaac caagaaatta ctggtgaaac cactttgaca aaagaagaca
     1621 aagataccgg tcatgaaact caagggaaag ctgagtttaa aagagctgaa tacaccttgt
     1681 ttactgttaa ggatggaaaa gcggtcaaat ggaccgagaa ctttaaacca gaattgttga
     1741 agggaacgaa agcttctgat gaaaccgtaa ctttgacttt agatgaaaag aatcaagttg
     1801 ccgttaaaca cttagcgatt aatgagtatt actggcaaga aaccaaagcg cctgaaggat
     1861 ataccttgga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa
     1921 atgctgtgat tactcgagat gttgcggcaa aagaacaagt gattcgcttt ggctttgatt
     1981 tctttaaatt tgctggatca actactggca ctgccgaaac tggcttcaat gatctaacct
     2041 ttaaagtgtc gccattggaa ggaaccaatg aaatcaccgg tgccgaagat gaagcgacca
     2101 cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg
     2161 gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag
     2221 aaatccgttc tacttttaaa gaaaataaag aggattatac caagagtgat tatatcttta
     2281 ctattaccga aaaagggcaa aagcaaccga ttaaaacggt aacggtccct tatgggaatc
     2341 taaccaatga gaatttctca gtcagtttga accgtttgat gctttatgat ttgcctgagg
     2401 aagaagatag cttaacttct cttgcgactt ggaaagaagg agataaagaa ttgactactc
     2461 ttgattctac cgagctagtc gataaattga gctataatct acatgaaatc aaagaagact
     2521 ggtatatcgt agctcaagcc atcgatgtag atgctacaaa agctgcccaa gaaaaagata
     2581 aaaaagccaa accggtggcg attgcggaaa caaccgcaac tttagcgaat aaagagaaaa
     2641 caggaacttg ggagattctg cataaattaa ctgtcgaaca agttttagac aagaccattg
     2701 tcttattcaa ctatgtgtat gaaagcaaag aagcctatga agctggtgat gagccggtag
     2761 cgaaggatgc tagcttgaac aatcaagcgc aaactgttaa ttgtatggtg gaacgtcatg
     2821 tttccatcca aaccaaagcg cacttagaag aaggttctca gacctttact catggtgacg
     2881 tggtagatat gtttgatgat gtatccatta cccatgatgt actagatggc tcaaaagaag
     2941 cttttgaaac aattctgtac gctttatttc cagatggtac aagtaaagaa atttggaaat
     3001 ctggcaagat tgattacgaa gtgaatgaca aagaatttac caaaaccgta cttgcggaaa
     3061 aagtggatac aagtaaatat ccagaaggta caagttttac cttcaaagaa attaactatg
     3121 acaaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaactt
     3181 taacgcccaa aaaagttccg acaacaccaa gtacaccaga acaatctgaa atgccaactg
     3241 ttccaagtgg ctctcaagaa tctagtccta cagtgaaggc attccctcaa accggggaga
     3301 aaacttccaa tgtgctactg ttcattggct ttagcttgat ctttgcgacg gctggctatt
     3361 atttttggaa tcgtcggaac taaggtgata agatgaaaca tgtaaatagt agaaaacaat
     3421 acgcgcgacc gcctcctaaa aaagtggtcg cgctattttt gtattcaaaa ctaacaatga
     3481 aaagaggaaa aaatatggaa ttgaaatttg ttgtaccaga tatggtcgag acgttcggaa
     3541 aactaacata cggtggtgaa ggtgaagttc tatcggaagg gtatggtcga aatactacaa
     3601 ttgttggtcg aagttaccat ctgttttcaa ataaacaacg ggccgatgat attgaagtgg
     3661 tggtagctgc ggaagccggc gaaaaggatt ttgaccaaga tcaaccacta aaagctgtaa
     3721 atccccactt aattgccaaa ggctatgaaa ttgagaatcg tgggtttacc gattacgtgt
     3781 tgtatgttga tgatttagtg aaagcatagg aggaaaagaa gatgagatta acagaaggta
     3841 ttgtcgtaga ttcaggatta acctttggca agttgcgttt ttccgcatta cgtcgagaag
     3901 tacgcaagca aaatgaggac ggaacggtta gtaacgaggt aaaagaacgg acctataatt
     3961 tgaaatcctc cgcgcaaggt cgaatgatcc aagtcagtat tccagcaaat gtacccttac
     4021 gcgagtttgc ttatgatgca gaggtagagc tggtcaatcc aatcgtggat acggtcgcca
     4081 attatgtttt tcgggaagga accaccgtta attggtttat caaggcagat gatttagtct
     4141 tgaaacgaca actgaatcaa ggaaaaccta cgaaccaaaa tgaaggaaag aaataggtga
     4201 agaatatgga aattaatgtt gaacatattt tagattgtct cgatcaattt ggcaaaggtg
     4261 agctaacgga ggaacaactc actaacgcat taacctatga tgaaaaaatg tttctgatta
     4321 tgcatcaagg gttactggaa gtaggtaacg atgccaaaga agactttgat gtactggatt
     4381 ggttagggga agaagagtct ttctttatgg ttgtcgaggt aaatgaaagt ttatgtcggc
     4441 aggcagaatc tgttttggaa gaaatcgatg tggagatgcc ggatgccatc gaaagatttt
     4501 taaaacaatt agtggaaacg aaacaacttc ccgtagtagt aaatgactag cataactttt
     4561 tgaaattgcc caatgaaatc ctattgggct ttttcgactg gaggtggaaa ccatcaaaat
     4621 gtacaaagga catcgcatac gcgcaggaga tcaacacttg atttatcact ttgttcttgg
     4681 ctggctaatc tttttgttca ttggttggat gagcgtcttt tatttacaag aacttagaca
     4741 atttgatatt tctaaggtat cattttccac tagtgaaatt ggctggtcta tgaaggatgt
     4801 aatctgcttg ctaggaagtc tcgctctttc aggaagcatg atgttacttt atatacactt
     4861 ttttcaggat cattggcgaa gtctttggca tcgacaaaag ttagctcgga tgattctaga
     4921 aaaccattgg tatgaagtaa agcagactca aggtgaaggt tttttcaaag atttgaatag
     4981 tagttggacc aaagagagta tcagttactt ccccaaaatt tattatcgaa tgaaagatgg
     5041 cttactttct attcgcgttc aaatttcact gggaaaatat caggatcagc tattgaagtt
     5101 ggaaaataag ttagaaagcg gactgtattg tgagttagta gaaaaagaac tcaaagactc
     5161 ctatgtggaa tacaccttgt tatacgatat gattgccaat cgaattggga tagaagaaat
     5221 agtggcagaa aatggggcgt tgcgattgat gaaaaatcaa gtatgggcgt atgattcctt
     5281 gccgcatatg ttaattgctg gcggcacagg tggtgggaag acctatttct tactcaccat
     5341 tattcaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcgga
     5401 cctcgccgat ttaggtacga taatgcctca cgtatactcc caaaaagaag aaatttctga
     5461 atgtgtggaa gatttttacg aacgcatgat agctcgtagt aggtcaatga aagaaatgcc
     5521 caactacaaa acaggagaga attatgcgta tctcggactt ccgcccaact ttttaatctt
     5581 tgatgaatac gtggcttata tgggtgcgaa tcgttttcct acaagcatcg aatagatgtg
     5641 aaatggaagc ctattgagat tatcaatagt gacaactaat gttttgagag atggaatgaa
     5701 agccttcaag ttgagctgaa acatccccgc taatactcct acatgctcta atcaatgaag
     5761 tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa atctgcctaa gtgcttgagc
     5821 ataaggctga ctatagggcg ggcggctgaa aaagataact atggtaagaa tgtctgaaaa
     5881 tgactgacga atctgcgaat gtacgggtct aaaaggctga tcttattgaa agataggaac
     5941 atacggacgt atggtgagat accgacaagt aaggttccat gatatgaaaa tcgggatagc
     6001 taacgaagaa gctataattt cacaggctca tagtagatac ctaaggttat acatgatagc
     6061 taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta aatgactact tctagatagg
     6121 tgcatataag taataacgaa attgctttgt ttctgtgaaa gtaagggcac agtaccgatg
     6181 aagcgtctaa caaatgtgga gggatagccc taagtctatt taataacaat gaatgttgaa
     6241 acagatgaag ttcaaggatc tggtaagaac gtgggaaaat ccgttatgga ggtgccacgc
     6301 ttgaacacaa aaatacgtta ctgggaatat tatgatctac aagaaacatt cgatcagcta
     6361 tttactcaaa gtagaaacgg gaaaaagttt tatcaattat atgagttaat catttctgaa
     6421 aataatattc ttttagcata ccgtactatc aaggcaaaca aggggtcttc gacaccagga
     6481 acggattcct tcacaattga taactataag gaaatgaacc aagcagagtt tatccatctt
     6541 attctcagtc atttagaaaa ttacaaacca aaatccataa agcgagtaat gatacctaaa
     6601 cccaatggtg agaaacgccc attaggaatt ccatgcatga ttgatcgaat gatccaacag
     6661 atgttcaagc agatactgga gccaatatgt gaagctaagt tttatgaaca tagctacggt
     6721 ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca ttatgtatct cataaacatc
     6781 agtaaaatgc actatgcagt agacattgat atcaaaggtt tctttgataa tgtaaaccac
     6841 cgtttactga taaaacaatt atggaatata ggaatttgtg acaaacaagt cttagctatt
     6901 ctttcaaaat ctctgaaatc acccattcaa ggagaaggta tcccgagcaa aggaacgatc
     6961 caaggaggta tcatttctcc actattatct aatgttgttc taaacgactt agaccattgg
     7021 gtctcaaagc agtggcatac ctttgaaaca aagtatccct atactaaagg ttacaataaa
     7081 tttcgtgctc taagagatac aaatctaaag caaggctata ttgtcaggta cgcagatgac
     7141 tttaaaatca tgactaatga ttatccgaca gcattaaaat ggttccacgc tgtcaagctt
     7201 tacctaaaag acagacttaa attagatatt tccaatgaga agtctaaaat cgtcaatttg
     7261 cgtaaatgta agtcggaatt tttaggattc gctatttgcg tgaaacaaaa agggaaaaaa
     7321 tgggtttgta attcacgtat ttctaacaaa aaaaaggatc agattaaaga agagattaaa
     7381 caaagaatca aagatattca aaagagtcct acagcacaaa atgctctcct gtttaatgct
     7441 ctcgtgctcg gtgttcataa ttatttcagg tatgccactc atattaattt agatttgaat
     7501 aggatgaact acgttctttc attcactact tacaaccgat tatcaaattg tagtacaaga
     7561 tgttacccca acaaagcttc cccaacttac aaaaagtttt actcaacaac gatgaaaacg
     7621 tataaagtag ctggagtcta tttgtaccct ctttgcgacg tatctacaaa aacaatttac
     7681 ggatttaata cggaggatac accgtttact ccgtttggtc gtcaaagatt ggagcacaaa
     7741 agcctacagt ctcttgtgtt ccaagaatta agaaaattga tggaatcgaa aataccgaat
     7801 agaacggttg agtatttaga tagtagaata tctcgttact caatgaaatc aggcaagtgt
     7861 gagattacaa aacaattttt gccagcaaaa gccgttcact gtcatcacta tttgccaaaa
     7921 agtcttggtg gagacgacaa attcgataac ttgagaatta ttcataagga tatccatctt
     7981 ctgatccata caacaaataa aatgataatc gatcactacg tgcatgaact gaaactggtg
     8041 ccagagcaaa tagcaaaaat caacctttat cgaaagatgt gtaatctgca aaacattcaa
     8101 tagaaaataa atagatggaa cgccgtatga ggtgaaagtc tcacgtacgg tgtggagcgg
     8161 gggaaaagat ggagattata tcaaattctt acctatcgct atagatgtta acgacaaaag
     8221 aaagtgcggt gattttgaat aagcttaaac aaatcgtgat gttaggccgt caatctggtt
     8281 tctttctgat tctagcttgt caaaggccgg atgcgaaata tctaggagat gggattcgcg
     8341 atcagtttaa cttccgtgtg gctttgggtc gtatgagtga gcttgggtat tccatgatgt
     8401 ttggagaagt cgataaaaat ttttttatga aacgcatcaa aggacgaggt tatgtagata
     8461 cagggggcag tgtgattagt gagttttata cgccacttgt accaaaaggg tatgatttct
     8521 taagggaaat tagcaacatt gttggattaa ccgttcatac ggaaagagaa aataatagtg
     8581 tgtagcctag gaatatcaat aatcctgggc ttttttattg aggtgattta tgacattttt
     8641 agatttattc gccgggattg gtggctttcg cttaggtatg gaacaagccg gtcaccagtg
     8701 tatcggtttt tgtgaaattg atgaatttgc tcggaggagt tataaagcaa tccatgatac
     8761 aaggaaggag gtggaaatgc atgacatcac aagtgtatca gacgaatttg ttcgatccct
     8821 cggaccagtg gacatccttt gcggcggctt tccgtgccaa gctttttcaa ttgcgggaaa
     8881 gcggcaagga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcgccac
     8941 tcttctccaa cctaagtttc tattccttga gaacgtccgg ggattgctca atcacgaagg
     9001 aggggctacg ttcgagacga tcctccgaac gttggatgga ttggggtatg atgtggaatg
     9061 gcaagtgctt aactcaaagg cctacgttcc ccaaagccgt gagcggatct tccttatcgg
     9121 acattctcga gatgcatgta ccgaacaagt atttcctatc attggatcgt cttcaacatc
     9181 tgatcaaaac atcagaaact tggtaaatat caatccttcc aatcgaggaa tgggtggcca
     9241 agtctatggt tcagatagcg tagcaccaac cttgaccggt gatgaaggaa ttaaaattgc
     9301 cttaccggta aaggatggga tttctgtagc gggtatgttg tcgggaaatt ttgaacaagg
     9361 caataggatt tatgaggtaa ccggaacagc ccccactttg tcaacgaaac aagggggaac
     9421 gaagattatg attcggaaaa atgaaacaga atatcaagag gtgaagccag gggacagtgt
     9481 gaacctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac aaagtgtgca
     9541 taccttacta acaggagacc aacaagcggt agtcacggat cagtaccaga ttcgaaagtt
     9601 aacacctagg gagtgctggc ggcttcaggg ctttccagat tgggcttttg atcgagcttc
     9661 tcaagtcaat tcagatagtc agttgtataa acaggccgga aactcagtaa cagttccggt
     9721 catttttgat attgctagaa gattaaaggg ggtaaaagca tatgatttat gacgagttga
     9781 ttggagaaat ctactggatg attggcaaga ttcaggcaga tccgaaattg gaagaagaac
     9841 tgtaccggct caattttgat attcgcaaaa acggggtgaa agtccaggtg attcgtatgt
     9901 agtggatgag gaaacggatg ctcgaatgga gctcaatcaa gtcatcggtg aattggaacg
     9961 aattgcggac cttgccaaag aacctgatat ccgccaatac ttgtttgaga taaaagcaga
    10021 gttggaaatt gagggtatca ccgcggagta atagggaaat aacccactga taggcactga
    10081 ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg tgcctgttca tggacggcgg
    10141 cagccggcca tgatgtctca ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaac
    10201 aagcaagtaa ccataaaaaa tgcttgttgt atcaaggata ttaagtaatt tgtataggtg
    10261 ttaactcatt tggaaaagtt aacaccttga atttggaggt gaaaacttgg cgcaacgaaa
    10321 tttagattat cggttattaa aagaacgacg caacgaatat ggcgtttcac aaaataaatt
    10381 agctactgct tgcggactta gccgcccata cctaaatcag atagaaaacg gtggcgtaat
    10441 cgcctctaca aagaccatga gaaaaatttt tgatcaactg gaaagtttca atcccgattt
    10501 acctttaacg ttactatttg actatgtaag gattcgcttt cccacaacgg atgtgcggaa
    10561 aattattcaa gaaattctcc atttgaaatt cgagtatatg cttcatgaag attacgcctt
    10621 ttactcctat caagaacaat atgtcatggg cgatattgta gtgatgttat ctcatgaaga
    10681 agataaaggt gtccttttgg aattaaaagg tcgtggttgc cggcagtttg aaactttttt
    10741 actcgcccaa aagcgtagct ggtacgactt ttttgaagat tgtctaaaag ccggtggcgt
    10801 gatgaaacgc ttggatttag caatcaatga tcgagtgggg ctactggata ttcctgattt
    10861 aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttca aaagttaccg
    10921 ttccggggaa ctgttgaaag ctgatgaaaa ggatggtatg ggcaatacct tatatattgg
    10981 cagcctcaaa agcgaggttt atttttgttt gtacgaaaaa gattatgaac aatatatcaa
    11041 gttagggatt ccattggacc agaccgaaac gaaaaatcgt tttgagattc ggttgaaaaa
    11101 tgaccgtgct tattatgcga ttcaagattt gttaaaaggt cgtagcattg aaagtaccac
    11161 gttttccatt atcaatcggt atttgcgatt tgcggataaa gtagatggaa aacggcgaac
    11221 caattggccc ttgaatgaac aatggggtcg ttttattgga cggaatcgga aagagattca
    11281 attaacttct gaaccgaaac cgtataccat tgaacgaaca ttaaattggc taggtagaca
    11341 agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcaaa caacgtatat
    11401 ccaggatatg gtacagaatg cgcgactctc ggaccggcat aaaaagattt tggagcaaca
    11461 aagtatggca atcgaaaact tgattgtatg aaaagaggaa ttttgatgga aattgtaaaa
    11521 gatatgttat tggtacttgg tggcagtatg cttggtgtta ccgtgatgtg cttgatgaat
    11581 gcaagtgcag cagcggattg agcaatggaa aagaaaggaa agtgacaaaa tgaatttcgg
    11641 acaaaattta tgtgcgactc gttccttagt gaaaagctaa ggcactaata aaagaacgaa
    11701 aaagtttggt tattaggaga actgataatc tgacaggtgg aatgatgaag taacgctctg
    11761 aaacgcctgc cctgagcctc cgacaagcat tgtatgagga aactcagaat gtttgaagct
    11821 cggtaaagac ggctgaaaga taccgtaata gttgatgaac atatatcttc tatcgaaaac
    11881 tatccagagg tgggtagcgt atccaacagg tcgggggtct ataaaatatc tatggttaga
    11941 atgttctata aggaactgac gaaacagcga atgtacgggt ctaaatttga actttgtaga
    12001 aatgcaaagg tacgagaaat cgtagctggt gtggcaaagt aagaatatag gttatgaaat
    12061 gccatataac gttacaggcg gtatcaagcc agcaggctta tagctagtga cctaaggata
    12121 tatatgtata gataagatta tcggaacgag gaaaggtatt gggttctcat caagagaata
    12181 aactgacgaa gaacaataag cagttgaact aatgctgaaa agcagagggc gaacctaaga
    12241 aattcttgta atgagaatgg aggaatgccc tcaagtcgtg tatatattag aaaagtatta
    12301 tgttgtttca ataaggatta cgagtaagat aaaaaagctc actcaatttc aagtaaagga
    12361 gtgaagcctt atgcctaaaa ataaaaatga aacattgtgt gtagaagatt taagacacgc
    12421 tgaatattac gaaatgcaaa acacctttga tgatttatat gccaaaagta agaatggaga
    12481 tattttcact catcttatgg atattatcct atcgagagaa aatatattac tggcatatcg
    12541 aaatatcaaa gcaaatgcag gcagtaaaac agcaggaaca gacggtacaa taattaaaga
    12601 tattggcaaa ttgccagcag aaacagttgt caaaaaagta agatacattg ttgcaggcag
    12661 tccacatggg tatcgaccta agccagtgag gcgaaaagaa ataccaaaac caaatggtaa
    12721 aaccagacct ctaggtattc cttgtatgtg ggacaggctt atacaacaat gtataaaaca
    12781 agtattagaa ccaatctgcg aagctaaatt tagtgaaaac agctatggtt ttagacctaa
    12841 taggtcagtt gaaaatgcaa taaaagcaac ctataaccgc ctacaaatca gccagctaca
    12901 ttatgtgatt gaatttgata ttaagggttt ctttgacaac gtgaaccatt ccaagctgat
    12961 aaaacagata tgggctatgg gaatacggga caagcacttg atattcattt taaaaaggat
    13021 attaaaagct ccaatcaaaa tgactaacgg tacaataaca tatccagaga aaggaactcc
    13081 gcaaggggga ataatatccc cactactagc aaacattgtt cttaatgagt tagaccattg
    13141 ggtagaaagc caatggcagg aaaatcctgt tacaaaaaac tatgtggttc atatcaataa
    13201 aagcggtagc ccatgtaaaa gcaacgctta caaagaaatg aagaaaacaa agttaaagga
    13261 aatgtatatg gttcgttatg cagatgattt tagagttttc tgccgctaca aggaaagtgc
    13321 tgaaaaagcc aaaatagcaa tcactcaatg gattgaacaa cgattaaaat tggaagtatc
    13381 acaagaaaaa acaagaattg taaatgttag aaaaagatac tccgactttc ttggtttcaa
    13441 gattaaaatg ataccgagaa gaaagaagtt agttgttaaa tcacatataa gtgataaaca
    13501 attcaaaaac caaaaagaca agctggtatc acaggcaaag aaaattgctt ctccctcaaa
    13561 agggaaaacg gaattagaag aaacaagact ttataattca atggtattag gtatgcaaaa
    13621 ttattatcag attgcaacga atgtaaatct ggattgtatg aaactgaata gagctgtaat
    13681 ggctatcttc actaacagat taggtacaaa caaaaggggc agattgagaa aaacaggcag
    13741 acaacttacg acaaaggagg caacaagata tggtaaatct aaaatgcttc ggtatgttgc
    13801 tggcgagggc gaacctattt tccctgttgg atatatccaa cacagaaaac caatggcaaa
    13861 ggtgtattta gcgaattgct acactctaga gggaagaaaa tttatccaca ccaatctaag
    13921 tgttgataaa ttactgatgt accagttaat gaaattatct ttggaaaatc gcacaataga
    13981 atacgcagac aatcgaattt cgctattttc tgctcaacat ggaaaatgtg ctataactct
    14041 ggaagaattt caacaagcta atgaaataca ttgtcatcat attgtaccaa ctggagctgg
    14101 tggaaatgac gattataaaa atcttatttt ggtaaaagag gcggtacacc gtctaataca
    14161 cgctaaaaca gaagaaacaa tacaaaaata tatagctcta ttgaagctaa ataaagtcca
    14221 gctcataaga ctaaataaat atagagtatt agctggcaat caagaattaa acttgatata
    14281 ggtctactac ataaatactt ttcaatataa acacgatgga acgcggagtg cggtgaaagt
    14341 cgcacgctcc gtgtgaatgg ggggaaaagt ttgagatatt tataatatca ggaacttacc
    14401 tatccatata tcaatggttt ttaaccaatg ctcaatcttt agtcttacta gcgattgtgg
    14461 tcattggttt gtttttagga tttaaacggg agttttccaa actgattggc ttcttggtga
    14521 ttgctttgat tgcggtaggt ctggtcttta atgcttctgg tgtgaaggat gtcctactca
    14581 atttgtttaa ccgaattgtc ggtgcctgag ggggaatcac aatggaacag atgcgtgttt
    14641 atattgcgaa ccttggaaaa tacaatgaag gtgagttggt gggggcatgg tttaccccac
    14701 ctgtcgattt tgatgaagtg aaagaacaga ttggattaaa tgacgaatat gaagagtacg
    14761 cgattcatga ttatgaactg ccttttgaga ttgatgagta tacgccaatt gaagagatta
    14821 atcggttgtg taacttagcg gaggaactag aaggaacgcc aattggtgaa gctgcttctg
    14881 agattcaaca cgctttcttc aattctttcg aggaaatggt agagcatgtg gatgatattg
    14941 tctattatcc agattgcgat gatatgagtg atgtggcgca ttacttgatt gtagaagctg
    15001 gtgattttgg cgaagttcca gaacaattga agccgtatat tgattttgat gcttgcggtc
    15061 gagatttgga gattggagga aattatttgg ttactagtcg tggagttttt gagtatccag
    15121 aatgaatcaa acactatata tagtgtttga ttagtatatg taagcaaaaa aacacaatat
    15181 atagagtgca aactgaaggg taatgtgata taatatttgc cgaagaaata tttttgaaat
    15241 gaggtaatat tatggcaaat attcaaacaa gttttattga tttccataat tcaattagat
    15301 tggacgtaga agacaatacg ctacttaagg actataaaga ccaagtaatt gatggattaa
    15361 aagattactt acctgatgat gtgaagtttg aaactttttt acagggaagc tattctgttt
    15421 ataccggaat aaaaagctgt gatgaaaaaa ttgattttga catcgatatt gcagttgctt
    15481 tcgaaattga tcatactgtt tatgaagatc caagggagcc taaactgtgg gtaaaagagg
    15541 cgctagttga aatttttcct aatgctcaag ttaatttaaa agtaccttgt gtaactgcta
    15601 catttactgg aaaaaagaca aaaaaaaatg tgcacgttga tgttgcggtt tatgctaaag
    15661 aagatgaaaa ttatttctta gcaaaagcaa aagaattcag tgctcccgaa aaccgatgct
    15721 gggaggaagc ggatcccaaa gtattaaaag agaaaattaa tagtcatgta gcagattcag
    15781 atgatcgaaa acaatttcga agatgtattc gctatttaaa aagatggaaa gataacaatt
    15841 ttaaccaaga atataaacca acaggaattg gacttacaat taatgtgatg gatacgttcc
    15901 tagttaataa gtccactgat tttttaacta gaaaagttca gtataatgat atggaatgta
    15961 tgaagcagat tgtttcttcg ctgaaagatt catttgtgta tgagtacagt gaaacagatg
    16021 gttggcatta tcgtctacat gcatatcttc cagtgaagcc taacagtgac acctattcaa
    16081 aaatgacagt gaatcagatg agtgatttta aaaacaaact ttctaaatta tatgatgatt
    16141 tgattttcgc tatcgatact gaagatgagt atgaagctac aaaacgatta aataatcaat
    16201 ttggggagga ttttttaatt atttcagaag aagaagttac agaaaaaaac ttaagaaatg
    16261 catttgttac agactatccg agtgcttagg ttggtgttaa agattggatt atactgatat
    16321 tttaaaagaa aagctaacag atgttgtgcg tttattcgat ggtaattact atggggattt
    16381 ttcatttgct acaattggaa agttgcgttt aaaaattgaa ataccaaaac attttccatt
    16441 ggtacttcct aggatttaca ttcataattt tgatcattta ggactttacc taccacatgt
    16501 agaaaaagat gggttggtat gtttttcttc agatataaat tatttagtag atgaagaaaa
    16561 ttcagaattg gttttattgc agtgtgttaa taaaactata aaaacaattg aggaaggttt
    16621 gaaagggtta aataaaaatg attttcgaaa agagtttgtt gctttttggg acaaacaaga
    16681 gaatcaaatt tcgttattct acctttgtga accagcgttg tttatcaagt cagttgatac
    16741 aataatgtac ggtaagtctt tggttgcttt ggataaagag aacgaattac aattaaatgc
    16801 agcgagtaga ttgtttaatg gagagaaaga agatactcat gaattagatg catactatgt
    16861 tccaattaga aacaagaata atattatgcc cccgaagaat aataaagcat ggtcaaagaa
    16921 ggatatttta caaatagtaa aaaaaaatgt aacaggtgca aataaaagat tttttttaaa
    16981 acttgaaaaa agaaagaata gacataatgt tttacttatt ctagatatcc caattgaaga
    17041 aaagtcaaat aatgtcataa ttggatttct ttttacaaaa caatctggat ctataaaaga
    17101 ttataagggt acgcctagga ctgtattagt tcagcgtcat gatgaattat atttggtgaa
    17161 tagaacgagc ggagaaaact atggattggg tgctaatata gctattgttg gggtagggtc
    17221 agttggcagt aaaattgctg aaaatcttgg tccattaggt gtgagaaaga tgacactcat
    17281 agacaatgat tgttttcaag ctgaaaattt gtatcgaaat tcacttggtg cagatagttt
    17341 aaaaaatgcc cgatctcaga ataatttttt taaagttgat ttaatgcagg aggagctcga
    17401 aaaaaaaatc ccataccttt gtgttgaagt ggagtcaatg aacgttttag acttaattag
    17461 tatgcaacct ggttttttca aggaatatga ctatgttttt gtttgcatag gagatacaat
    17521 ggcaagcctg gctttaaata attatttttc caaaagtagt caaaaagttt tctattcttg
    17581 gctggagcca tatggcattg ggggacacac tctatttata aattattctc taaatcaaaa
    17641 aggttgtttc aattgtcttt atacagattc aaactctaaa aataggtgtt caaatagagc
    17701 ttctctggta aaagaaggac aaatttttga aaggacttta gcgagttgcc gctcaaaatt
    17761 tataccatat aatgctattg cctcgacgga atgtgcaatt aatgcaataa aacttttcca
    17821 taaagtcaat caaaataata tgaccaatag tactcttttt tcatggatag gagatgggag
    17881 tacttttgag aatttaggat atcaatatag cgacagaaat acttttctta atggagtcaa
    17941 taagtttgaa gaaagattct tttctaaata ttgcaaagta tgtggggagt tagatgatta
    18001 aattttcgtg tgaccgtatt gtcattgaag taagggatga agttcttaag atatttgaac
    18061 aatatcggca gggaaaggat gacttgcctg aagctggagg agttcttcat ggaaaaagac
    18121 ttaagaatgg gagcataata attaccgata taacaacacc tcaaatagga gatatacaaa
    18181 aaagattact ctttaaaaaa aaccgagaaa ttcatcaaca gctttctaat gagaaatgga
    18241 tagaatcaaa taagttttct attgcattgg gagaatggca tactcatcct gaagctgtac
    18301 caactccatc aaatgttgat aagaaaagct ggagactgaa cgtgagcaag caacatgatg
    18361 atagagttta tgtttttatt atcgttggat tttctgattt gagaatgtgg ttactgtcaa
    18421 aaaaatcaga tattaggcag gctataaaac tataaaggag tgattaggtg aaatcacaaa
    18481 agaatatttg gtggtggggg tttttagtag gaaaggtaat gataccagcc cttctcttaa
    18541 tttctctacc gatgattact tttttcttta ccaataaaaa aatgagtaaa gaagccgtga
    18601 tttttttctt tggagaccaa aaagctacct ttattgaaac gttagtaaca tctcttcagc
    18661 ttaatttgaa ttacattttt tcaattatta ttgttatatc gttattgaat ttttttaaaa
    18721 aaagaaattc aggaaaggtg tttaactcta atggaaatgt gtactacaac tatttttatt
    18781 ttgtattttg ggtagcagca actttacttg gttatgataa aattcagatt gcaggaattc
    18841 ctatacacat gcaatataag ttggttttga gtggaatttt ttctgaggtg ttgccagata
    18901 tatacgatga tcattacgac agtgatggaa catgtaaagt aagcattgaa aaagaaaatt
    18961 tcgatgacat cgatggatat gactctgtga acttactgat tattgatact tacgatataa
    19021 aaatgagtga attatctatg gaaaatcaaa catatcctac gataattgtt agagggaatt
    19081 caatagatgg agttcgtaaa gttaatagat ctttaatctt ggaaataaaa aaaacaatgg
    19141 atgaaattca aaaaagtgac tttaaaaaag tatttgttgc ttcaacatct aatccaaaaa
    19201 atagtatcaa tattattaat tcatcgttta gatttttcgg tcgatcacgt aggtttaaac
    19261 tttatgttct tcaaaaggat tatgcaagca atggaaagta tagtaagaaa tatagaattt
    19321 ttatttaaga aggaattttt ccttcttttt attttggagg aaactatgaa aaaaataaaa
    19381 agctacacca gtatttgggc agttgaaaaa gtcatttatg cgattaatga ctttcagctg
    19441 ccttttcctg tgacctttaa ccagatggct tggtttgttt tatccttgat gtttgtcatt
    19501 gtatttgccc atgtgcctcc gctatccatg atagaggggg cttttttaaa atacttgggc
    19561 attccgatag cggtcacttg gttcatgtcg caaaagacct ttgacggtaa gaaaccattg
    19621 ggatttttac gatcctttat cagctatcac ttacggttta aggtcacttt tgcaggaaag
    19681 aaagttaaag aacagaagaa acgatgggac gaaccaatca ccttggtaag gagtgtgaat
    19741 tatgtacccg attaagtata ttgaaaataa tcttgtcttt aaccaagaag gcgagtgttt
    19801 tgcttactat gaattagtgc cttacaatta ctcctttttg tcacctgaac agaagtatca
    19861 agtacatgat aactttcgcc aattaattgc gcaaaatcgt gaaggtaaga tccatgcctt
    19921 acaaattgcc acagaaagta gtattcgtgc cacacaagaa cgttcgaaaa aggaaattac
    19981 cggtagactc aaagaagtag caaagcaacg aattgattta cagacagatg cattagtatc
    20041 catgattggc gatagtcaga ttgattatcg cttctttatt ggctttaaac tgatttcgac
    20101 agatgaagag gtcaatctaa agaatctcag gaaatctttc ttttctggac ttcaagaatt
    20161 tgtctatgga gtgaatcatc atttgatggg tgattttgtt tccttatcca atgaagaaat
    20221 tcgtcgctat accaagcttg aaaaactaat ggaaagtaaa ttggctcgaa gatttaaagt
    20281 caaacgagta acgccgagtg acttgacgta tttaattgaa catatctatg gtgaaaaagg
    20341 cacaccgttt gaagaatatg aattccagct accaaagaaa aagctgaaat cagaaacgct
    20401 ggtgaaacgt tacgacttat tacgtcccac tcgttgtttg attgaagaaa agcctcggta
    20461 cttacggatg gaacatgaaa atcatgagtc ttatgtagcg tacttaacaa ttaataccat
    20521 tgtaggggaa atggagtttc cttcctcgga acttttctat taccagcaac aacaatttac
    20581 gtttccgatt gatacttcaa tgaatgtgga aattgtgacg aataaaaagg ccctagctac
    20641 cgttcgtaat aagaaaaagg aattaaaaga tttagacaac catgcctatc aatctgataa
    20701 tgaaacgaac tccaatgtat tagatgcttt ggattccgtg gatgaattgg aaaccacctt
    20761 ggatcagtcg aaagaatcca tgtataaact aagctatgtg gttcgagtga gtgctaaatc
    20821 agtggatgaa ttaaaacgcc gctgtgatga agtgctcgat ttttatgacg ataccaatgt
    20881 aaaactagtc cggccctttg gcgatatgat gggtctacac aaagaattct taccctcaag
    20941 taagcgatac atgaatgact atattcaata tgttacgtct gactttttgg cgggacttgg
    21001 ttttggggct actcaaatgc taggagaatt agaaggcatt tactttggct ataacgtaga
    21061 tacggggcgc aatgtttacc tgaaaccggc attggcatca caaggggtca aaggctcagt
    21121 aaccaatgct ttggcatctg ctttccttgg ttcgttaggt ggggggaaat cttttagcaa
    21181 caatctctta gtctattatg ccgtcctttt tggtgggcaa gctgtgattg tcgatccaaa
    21241 aggagaaaga ggcggttgga aagaaacgtt gccagaaatt gcggatgaaa ttaatattct
    21301 taacttgacc agtaagccag aaaatcaagg cttacttgat ccctatgtga ttatgaagaa
    21361 aaagaaagac tcagaaagtt tggcgattga tatcctgacc tttttaacag ggatttccag
    21421 tcgggatggg gagaagttcc ctgtgttacg caaagccatt cgaacggtta cccaaagtga
    21481 tcagcgagga ttattacaag taatcgttga attgcgtaaa gaggactcac cagtcgcgga
    21541 aaatatcgcg gatcacatcg agtcctttac cgattatgat tttgcccatt tgcttttctc
    21601 agatggttcg attacccagt cgattagttt agataagcag ttaaatatca ttcaagtagc
    21661 ggatttggtt ttaccagatg cagaaaccac ttttgaagaa tataccacca tggaattgct
    21721 gagtgtttcc atgctaattg ttatttccac ttttgccttg gactttatcc atagcaaccg
    21781 agaaattttc aagattgtgg acctggatga ggcttggagt tttcttcaag tagctcaagg
    21841 aaaaacgtta tcgaataaat tagtgcgtgc tggtcgggcc atgaatgccg gtgtttactt
    21901 cgtcacgcaa aacagtgatg acttgttgga tgaacgtttg aagaataata ttgggctaaa
    21961 gtttgcattt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggatt
    22021 agataaagaa gatgaaagta atcaaaaatg catgcgagat ttggaaaatg ggcaatgctt
    22081 gatgcaggat ttgtatggtc gagtaggtgt ggtccaagtt catccagtct ttgaagaact
    22141 tttccatgct tttgataccc gtccgccagt tcaagagaga atggagtgag gagatgaaaa
    22201 agaaactttt tcgtatcttt ggaattgttt taatcagttt ggtcactctg ataatacttt
    22261 tgagcttagt tggaacggta gctgaggcaa caggtctggt tgatgataca gttggagccg
    22321 gaaatcttta ttcccaatat tcacttggta actatcagtt ggatttcttt gtggatagtt
    22381 cgtgggattg gctgccttgg aattggggtg atggcctagg gaagagcgta atgtatggac
    22441 tttatgccat tactaatttt atttggacag ttagtctcta cttatcaaat gccacgggct
    22501 acgtggttca agaagcctat aagctggatt tcatttcaga tacggctgaa agtattggga
    22561 agaatatcca aaccttagcc ggcattaccg aaaatggact ccagacctcg ggcttttact
    22621 ttgggtttct attattgatg attttagctc taggagttta tgtggcgtat acaggattat
    22681 taaaacgaga aactaccaaa gcagtccggg ctgtcattaa ttttgtgatg atttttctac
    22741 tttcgggttc gttcattgcg tatgcgccca cctatatcac aaaaatcaat gattttagct
    22801 cagatgtcag cgaagcggct cttagccttg gaaccgagat tgtcgtgccg aattcagaaa
    22861 gccaaggaaa ggacagtgtg gatttaatcc gagatagctt gttttccatt caagtccaac
    22921 aaccgtggtt actcttgcaa tttgatgatt ccaatataga agaagttggc gaagatcgtg
    22981 ttaacaaaat cctgtctgta agtccagatg aaaataaagg caaggatcgg gaagaagcgg
    23041 tcaaagcgga gattgaagat aatgacaatg cgaatctcag tattaccaag accatgaacc
    23101 gcttagggac cgtggtcttt ttggtgttgt tcaatattgg catatccttc tttgtctttc
    23161 tcctaacagg gattatgttg ttctcgcaaa ttctctttat tatctttgcg atgtttttgc
    23221 cgattagttt cttattgtcc atgttgccga cttatgagag tttgggcaag aaggcgatta
    23281 ttcgcttgtt caatacgatt atgatgcgag caggagttac tttggtgatt acgactgcat
    23341 ttagcatctc cacgatgttt ttcaatattt cggcaaccta tccatttttc atggttgctt
    23401 ttctgcaaat tgtgaccttt gccggtatct atttcaaatt gggcgacatt atgaatatgt
    23461 tcaatctcca gagtaatgat agccaatcaa tgggaagacg ggtcatgcga aaaccgcaaa
    23521 tgttaatgaa tcgaacattg cggcaactca atcgaaatgt gggacggact ttggcttttg
    23581 gtggtggtgc agcagttgga aataagttgg caaaggaaca atcaaaatcc aaatttaagc
    23641 catccggttc ttctctacga aagaattcac gattaccgaa cgatcacgag ataccatctg
    23701 acttgaataa ggaaaagcca attccaaata ataagaaaca atcccgaatg aatttggccg
    23761 ggagaaaagt cggcaaggtt ctggataccc aagctttggt gaaagataag accaaacaag
    23821 taaaggatgg agtacggaat acgccaacca acttgaaata caacttccat aaaggaattg
    23881 aaaaaacgaa aaaagcaccg gaagagttta agcgaggact ggtccaagaa aaagcaaatc
    23941 gaggagagtt gcgagaaaaa caacaacaac gtagagacga aaaaatagct gaaaagcgca
    24001 aagtattgaa cgaaataggg aatcctcatg ggaaaagaag ggaaaatgtt ccgataaaag
    24061 caaaggtcca atctcaaaag gatagagcac caaagcggat tgtgagagca aattcgaatc
    24121 ccgaaatcaa acgaaaattc gctagccaag aaatcatttc aaaaggaata aaccaatcct
    24181 caagtaagaa aaattctttt caacaagtga aacggcggtc tactctgtcc aaacgaacca
    24241 atcgaaaagt acaaaaaagt ttgaaccctc atcccaaacc aaggtcaggt gagaaaaaat
    24301 gaacgtaaag aaattaagtg ccatttcgtt aactttccta acaatctctt ttatgggtat
    24361 cttgatgtgt gtgggcttat tgtttggtga agacagtgaa ggaagtagta gttcaggtgc
    24421 tcctcctggg gggagtagtg tctcagaaga ggttttgaaa caccgtgcaa tggtggaaaa
    24481 atatgccaaa gaatcggata taacagagta tgttccgatt cttttagcga ttattcaagt
    24541 ggaatctggt ggcacaatgg aagatgtgat gcaaagttct gagtcagccg gtttaccacc
    24601 gaattcatta agtaccgagg catccatcaa acaaggatgt ctctattttg cgtctttggt
    24661 taagcgttcc aaagaattag gttgtgatca agatagtatc attcaggcct ataactttgg
    24721 cggtggttat ttggactatg tggcaaaaaa cgggaagaag catagttttg ctttagctga
    24781 atctttttca aaggaaaaat cttgcggtca aaaaatcgat taccctaatc ccatcgcgaa
    24841 taaggaaaat ggtggatggc gctataacta tggcaatatg ttttattgct tattggtgaa
    24901 gcaataccta actacaacac agtttgacga taaaacagtc caagggattt ttgacgaggc
    24961 gtttaagtat gaaggaacgg cttatgtatt tggtgggtca agacccgaaa caggatttga
    25021 ttgtagtggc ttgactcaat ggtgttatgc caaagttggt ctgaaacttc ctcgggtggc
    25081 tcaagatcag tatgatgcca tgacccatat tgacctaaaa gatgccaaac caggggattt
    25141 ggttttcttt cattcgactt atgatgccgg tacttatgtg acccatgtcg gtatttatgc
    25201 aggggagaat cgcatgtttc atgcaggaga tccagtaggc tggacgaatc taacagaaag
    25261 ctattggcag cagcatctta tcggtgctgg acggtacaaa taagagaaaa gaggaaaaag
    25321 aatgaagata aaaattgaac ggaatcaaaa ggaaaagagc ctaaaaaaga aaaaagaacg
    25381 agtcgtatcc gtgggtaagc atcgaaaaat ggtgcttgcc ctttggcttt tattatcttg
    25441 tagtcttact tttggcgttt ataaaaattt caccgccatt gatcaacata caactcatga
    25501 aaaagtggtt attaaagaga aagtagtgaa tacttctggt attgaaaact ttacgaagga
    25561 tttttcgaaa gaatattttt cgtggaaaaa taacaaagaa gcaattgaaa agagaatgaa
    25621 caacctcgaa cagtacctta cagaagaagg tttcgcttta agtcaagata tggttcgagc
    25681 agatattccc actaattccg aagttcagtc agtaaaagtc ttagatgttg aaaagagctc
    25741 ggaggaattt gtcgtttcat ttttagtgga gcaaaagatt acagagggaa aaaaggcaca
    25801 atcgatttct tctgcatacc gagtgacgat ctttgaagat gaaaacagga attatatagt
    25861 aacgagtttg ccaactatga ttgcaaaacc tgatagggca aaatacaaga caaaacaagt
    25921 agaaaatgat tcgaaaattg atgccaaaac tacagaagaa atcgctgagt ttttggaaac
    25981 gttctttaaa ttatatccat cagcttctga aaaagaattg gagtattatg ttgaaaatga
    26041 tgcgatgcga ccaatcaata ttgacttaaa gttttcgaag ttagaggatc cagtttacct
    26101 acaaaacgga aaaaaaattc tagtgagtac aaagataaaa taccttgata atgtatcaaa
    26161 aaccactagc tactttcagt acaaattaat attacaaaaa aatggaaact ggaaaatcat
    26221 tgatgttaaa taataatgac tcaatctaaa tttatgagaa gttataagta gtttaactta
    26281 tttcaggcag aagtcgccca tcctctatag gtgggtgatg aatgccgttc ggtaactcga
    26341 aaaccgacaa ttaagacata gtgccttttt attttaatcc atgtcataat cagtctatat
    26401 aagaaatggt tgatatacca tggaattaga tacaaataat cattcattat ttcttctttg
    26461 ttaccacctt attttagtag tgaaacattg taaacatctc attgatgatg taatgttagc
    26521 ttatgcaaaa aactatcttt gaaagaatat cagaatcaca tcacattacc ttagttgagt
    26581 gaaatcgtga taaagactac tttcatatta tgctcaaagc acaacctaaa accgaattaa
    26641 caaaattcag caatgcctaa gctcatgtct gataaaatga gactttctaa gggtcaaaaa
    26701 tttcctttga atacgatcat tagcttagat aaaagtaaaa accttagaac tacggggata
    26761 gcttggttaa taagagatat cgctgttaat aaagacataa gcttgcaagt atgctctatt
    26821 cccaagaatc tcccacttca agcactaagg atcgttaaat tcagctaagt ggcaagtagt
    26881 tcactagacg ataattatct ccatatttca atctcagctt ttccattttt catagcaaat
    26941 tccgttattt ttatagcatc taaaagattc agcttttcca ctgatctttc accacttttc
    27001 agctttttta ttgtgcttaa attgatacca gtaccttttg atattgaatt ggaagtaaaa
    27061 tcattaaggg ttttttttat taaattcata tccgcaattc cagcgttcat aagaatgaag
    27121 attctcctct catagactac tttgaaaatg gtactacagt ggctgtttat atatttcagt
    27181 aggttactgt agtggtcatt ttattataat tagaaaagga acctaaatca aaaatgctaa
    27241 agaaaatttt catagtatta aacctatcat gactaaagcc acggaattct tgataaaaga
    27301 gtatacttgc aagcctatgt ctttactaac agcgatatct cttattaacc aatctatccc
    27361 cgttgttcct aaggttcttg atgttttcta aggcaatgct tgtatacata gcccttgatc
    27421 aaaatattga tactaacgtt gacgtttcga tcatgatgtg tacggcaaat aggacaagtc
    27481 cattctcgaa ttgtgaatga cttcttacca tctgtgtgtc cacattctga accaatttga
    27541 ctagacggaa atcatttatc tatcttgatg atttttttga cagaagtatc gaaaatcttt
    27601 acataatctg ggtgttgcat tcttatgcag atgagagcgt tagctccgta ttcataacca
    27661 gttgcctcta aaacgtagtc catcggtgga tacctagctt gcggttgcca gtggctttcg
    27721 cagtcttctt gtaatcaaat cgagcatcta attgagtact agtattttga agataatact
    27781 tgagtttttc ggttttcaga agttcacttc atcttccatc atccatggaa atcccactca
    27841 cccatacgca ggcctccaaa aattcttcaa caatggtttt tcagacattt ttctgaatac
    27901 ctccttgttt tttgatacgc cagatagtca ggaaaaaggc aacaaaacac aaaacaaagc
    27961 tgcctaaaaa ggtaatcttc attccatata aaaagacatc cggccgatct gcgatatagt
    28021 tagttactcg ttggcccatc ttagcagaca tcgcttgata taagaccgtc gtagaaagcg
    28081 aaatcccgat gaccatcccc aaatttcggg caaaggaatt catggagcct gccagtccca
    28141 aattttcgat agtgacagaa ctcataacca tcgtattatt tggggactgg aacaaggcat
    28201 tgccggcacc catgatggca gtagcgacaa tgtacagcca caaaggagag tccactgaca
    28261 aaaacatata catgacttgg gttaccgaca ataaacccaa gcctactaat acaaaaatct
    28321 ccgggccaac ccggtcggtc aggtatccgc ttaaaggcgc cccgacgacc atcatcaagg
    28381 gaaagaccat catcaacagt ccggcatagc taggactcaa cccgcgagca ttttgcagat
    28441 aaaaaggaat cactacatta acaaagaagt tggaagaaaa aatcaacaag gctgtaatca
    28501 agctcatggt aaaaatcttg ttcttaaaaa taccgaaaga aatcaatgga gaggctgctt
    28561 tcttttcaac ttttaaaaag ctgacaaagg caatcactga aattacgaac agtacaaggg
    28621 ggactggatg gaaaaagcca ctttcttgcc ccaaaaagat tgttccaaaa aggctgataa
    28681 tcgtagccgc aatggcagca aagccccctc catcgattgt atggccgctc atggtgatat
    28741 cttttggtaa gaatttttca gcaatcaaca tcgttacgat tcccaccggt acattgatcc
    28801 aaaaaatgta cgcccaagaa aattgggaca aaatcaagcc gccaagacct ggtccagcaa
    28861 tcgagcctaa tgaaacaagc gcaccgattg ctcccaatgc ccgaccacgt tcatgtatgg
    28921 gaaagacttc cgtgatgatt ccggcatttg tagccatagt catactagca ccaatggctt
    28981 gtaccacccg tgcgaacaat taaataccta gcgaatgatt gaacccacac aacagcgagc
    29041 ccaagacaaa gatcagcgta ccaatccgaa atactttgat tttcccacag ctatcgccaa
    29101 ttttaccaaa cagcaacaaa caagaacaga caatcatcag ataaacggac actacccatt
    29161 ccgcttgatt cattggtatg cccatatcat ctgagatggt aggtagtgca atattgacaa
    29221 tactgccatc caatgtggac atgaaggtaa acatagatat agcaatcaaa atccaccagc
    29281 gatttttttg gacttttgca tcttcttgat aactatgcat aataggattt cctttctaat
    29341 gatcgtgtag ctaagaacaa tataacctat gtctactgcc caacaacttc tcgcgactct
    29401 agccatagga agcttgagta atttcgattt gttattctca tgtaaccatg taagtttgag
    29461 atgctttatt tgaacttact ctatagtaac tagtgataat tcgctttact ttcttctagc
    29521 aatttctaaa agtcgacttc attgttcttc agattaatag tgtgagctat ctcgtgagac
    29581 gtaagaaaat ttccattcta ctttttccca attttttcat atttatggtg ttgcacctat
    29641 ttgttttcca atttgctttt caaatacatt gctatgatat tcaattccat aggattatac
    29701 taaatgtcaa tagtatgaat aatatattat agatgtttat atatcactgt taattattat
    29761 ccacaataat taatgattac ctcgatattt gtcaatcaaa ggttgaaaca atttattttc
    29821 agcatctttt ctaacttgag ctgcactctt aatagaatca tacgatccta aatagtacct
    29881 ctttttttta aagttatata cgcaatccat ttctctttag aacgataaac tcccttaaca
    29941 ccacttgtat tatttttcgg taattttgac gttagacact ctattactgt cccttcaaca
    30001 catgtccttg tttcttgcct ttttaaacat ccacaacttt gagtatgtcc aaaaaataag
    30061 ttactataac ttatctcaca catactgcca cactcacatt gacaaaccca ttttgttttc
    30121 catgaacagt tattgtccaa aggatacttt gcagtaagta atccgaattt tttattttca
    30181 atgtgatgct tttttgattt caaacatcca cagcttttta catcaccttt tagaagtcgc
    30241 tttgccgata ctaacttttt tctcccacac agacattgac attcccaaac aacagttcct
    30301 tcacttcgca gtttagttgg cttgattgca actaacttac caaaagtttt atttgtcaaa
    30361 tttttatatt tatgcctatt tttataattt ccacatccac aacttttggt tctacctgat
    30421 agtaagtttg atcgatatac aatcgtttca ttcccacagt cacataggta acgatatttt
    30481 ttatatccat tatgagtaat ctcctcaaca acagtaaggt gaccatatct ttccttcact
    30541 gcacctaccc tcccaagaaa gaagtaccat ataatttcaa ttaactttta ataaatcaga
    30601 aaaaaatatt ttcaattttt cttagtctga ttttatgact aatttataat acaagactga
    30661 acgatacgta cacgaaatcc tcaagataga taaattgttt tcatctagta aaacctgctc
    30721 aaaatatgag cataaagacg gcaataatac actcgatatt cgataatgga cttgttttat
    30781 ttgtcaaact catcgtaatc gagatatcaa ttccagtctc aatatcctga tcaaaggtct
    30841 aagaatccaa ccattttctt gttactttgt cagattgatt acctttttta gtctccgaat
    30901 aataaggttt tcggatcaac taacatgctc tgagaaagac tactatcttt gcaaatttcc
    30961 aaatgaaggt gtgggccagt actattccca gtacttccta catagccaat tatttgtcct
    31021 tgcaaaacat tatctccagt ttttactagg tattgttgct ggtgagcata taacgcggtt
    31081 gatccatctt cgtgttgtat caccacgtaa tttccccaag agtagtgaaa ctctgcttta
    31141 acaaccttac cagctttaac tgcatgaatc ggctcaccct gtgtagctgc catatcaagt
    31201 cctcggtgaa attcatttcc acgatttcca aacaaactgg ttattgaata attatgaata
    31261 ggcacttggt atccagtctt actcatttgt ggacctttaa cttctttatc atagttggtt
    31321 aaatcatagg ttttaattaa ttcatttagc ttcaaatagt attgtgtatc tgttgcatac
    31381 cgtcctgtaa ggaatttagt tgcttctcga tatgttttac tattagcttt taatgctcct
    31441 gcataaaaat ctttatttcc actaattccc ttagttaata atttggcata atcttcaaaa
    31501 ctttcctcat agttttcata cactctaaat tttgattggg tcgtataaag cgttccatta
    31561 cccaaatctt cgtttgttac cataatgaca aagtttccat tataagttcc ttttatccca
    31621 aataaattgt aattcggtgc ctgagctaac tggctttgac cactggcaga ttctaagatc
    31681 gcttgtgcaa tcataacaga agcatataaa tcatatttct gaccaatttt tctcgcagac
    31741 tcaccaattt tcataataaa attttctaca gattcatttt tgtcaaaatg aatttcttca
    31801 ttaccagata gtattggttg tgattggaaa gattctgttg attctatttg ctgtggtgtc
    31861 gaaaaatttt ttgtttgtcc ttccgttttt ggcgcttcct tttttgaaga ctctatatgg
    31921 tttggtcttg tcactttcga agaagttatt tcacttgtac tacttgaagt tgttgaagat
    31981 tcggcacttg aaatcgtact actttcactt gacgaagttg ttgaagactc ggcatttgaa
    32041 gtcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    32101 tcacttgatg aagttgttga agactcagca tttgaagtcg tactactttc acttgataaa
    32161 gttgtcaaaa gttcactact ctctgctgtt acatcggaat tactcatttc agtcgtctcg
    32221 gttctttctt ttgcaatttt gtatgttgaa ttttcgcttg ccaacacatg gaaaggtgtt
    32281 gctataaaga gttgggacat taatataact gctgatgcaa gttttaaaga ttttttcatt
    32341 tgatttacct ctcttttttc tctttaatag tgttttc
//
LOCUS       DAZKAO010000019        31336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000019 DAZKAO010000000
VERSION     DAZKAO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.019"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8H8_000699"
     CDS             147..1016
                     /locus_tag="ACG8H8_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8985792.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8H8_000700"
     CDS             1225..2250
                     /locus_tag="ACG8H8_000700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8985793.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8H8_000701"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8H8_000701"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP8985794.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACG8H8_000702"
     CDS             3333..5036
                     /locus_tag="ACG8H8_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8985795.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8H8_000703"
     CDS             5038..7071
                     /locus_tag="ACG8H8_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP8985796.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LVPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8H8_000704"
     CDS             7082..7522
                     /locus_tag="ACG8H8_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8985797.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8H8_000705"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8H8_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985798.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8H8_000706"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8H8_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8985799.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8H8_000707"
     CDS             9793..11559
                     /locus_tag="ACG8H8_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP8985800.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8H8_000708"
     CDS             11574..12011
                     /locus_tag="ACG8H8_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8985801.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8H8_000709"
     CDS             12024..13175
                     /locus_tag="ACG8H8_000709"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP8985802.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACG8H8_000710"
     CDS             complement(13237..13935)
                     /locus_tag="ACG8H8_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP8985803.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACG8H8_000711"
     CDS             complement(13928..15280)
                     /locus_tag="ACG8H8_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP8985804.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACG8H8_000712"
     CDS             complement(15503..16903)
                     /locus_tag="ACG8H8_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP8985805.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACG8H8_000713"
     CDS             17064..17801
                     /locus_tag="ACG8H8_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP8985806.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACG8H8_000714"
     CDS             17926..18405
                     /locus_tag="ACG8H8_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP8985807.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACG8H8_000715"
     CDS             18402..19169
                     /locus_tag="ACG8H8_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP8985808.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACG8H8_000716"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACG8H8_000716"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8985809.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACG8H8_000717"
     CDS             complement(20072..20674)
                     /locus_tag="ACG8H8_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP8985810.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACG8H8_000718"
     CDS             20780..21010
                     /locus_tag="ACG8H8_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985811.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACG8H8_000719"
     CDS             21001..21249
                     /locus_tag="ACG8H8_000719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985812.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACG8H8_000720"
     CDS             21355..21939
                     /locus_tag="ACG8H8_000720"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP8985813.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACG8H8_000721"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACG8H8_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP8985814.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACG8H8_000722"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACG8H8_000722"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP8985815.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACG8H8_000723"
     CDS             23840..24019
                     /locus_tag="ACG8H8_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985816.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACG8H8_000724"
     CDS             24041..25066
                     /locus_tag="ACG8H8_000724"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP8985817.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /gene="glyA"
                     /locus_tag="ACG8H8_000725"
     CDS             25241..26485
                     /gene="glyA"
                     /locus_tag="ACG8H8_000725"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP8985818.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="ACG8H8_000726"
     CDS             26638..27585
                     /locus_tag="ACG8H8_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP8985819.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="ACG8H8_000727"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="ACG8H8_000727"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP8985820.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="ACG8H8_000728"
     CDS             complement(28487..28729)
                     /locus_tag="ACG8H8_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985821.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="ACG8H8_000729"
     tRNA            28850..28922
                     /locus_tag="ACG8H8_000729"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="ACG8H8_000730"
     CDS             complement(29041..29679)
                     /locus_tag="ACG8H8_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP8985822.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="ACG8H8_000731"
     CDS             complement(29802..30434)
                     /locus_tag="ACG8H8_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP8985823.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30785..31084
                     /locus_tag="ACG8H8_000732"
     CDS             30785..31084
                     /locus_tag="ACG8H8_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8985824.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10698 a         5457 c         6302 g         8879 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggtgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
    30601 aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc
    30661 gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag
    30721 atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat
    30781 cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt
    30841 aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga
    30901 aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc
    30961 aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg
    31021 aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt
    31081 ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc
    31141 taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt
    31201 tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg
    31261 ttaattaaaa gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt
    31321 taaaaattgg aaatat
//
LOCUS       DAZKAO010000020        29116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000020 DAZKAO010000000
VERSION     DAZKAO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.020"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1037)
                     /locus_tag="ACG8H8_000733"
     CDS             complement(<1..1037)
                     /locus_tag="ACG8H8_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8985825.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTR"
     gene            complement(1119..1808)
                     /locus_tag="ACG8H8_000734"
     CDS             complement(1119..1808)
                     /locus_tag="ACG8H8_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8985826.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1914..2282)
                     /gene="rplL"
                     /locus_tag="ACG8H8_000735"
     CDS             complement(1914..2282)
                     /gene="rplL"
                     /locus_tag="ACG8H8_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP8985827.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2335..2838)
                     /gene="rplJ"
                     /locus_tag="ACG8H8_000736"
     CDS             complement(2335..2838)
                     /gene="rplJ"
                     /locus_tag="ACG8H8_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP8985828.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3042..3169)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3248..3937)
                     /gene="rplA"
                     /locus_tag="ACG8H8_000737"
     CDS             complement(3248..3937)
                     /gene="rplA"
                     /locus_tag="ACG8H8_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP8985829.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4041..4463)
                     /gene="rplK"
                     /locus_tag="ACG8H8_000738"
     CDS             complement(4041..4463)
                     /gene="rplK"
                     /locus_tag="ACG8H8_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP8985830.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4806..5474
                     /gene="sdaAB"
                     /locus_tag="ACG8H8_000739"
     CDS             4806..5474
                     /gene="sdaAB"
                     /locus_tag="ACG8H8_000739"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP8985831.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5487..6359
                     /gene="sdaAA"
                     /locus_tag="ACG8H8_000740"
     CDS             5487..6359
                     /gene="sdaAA"
                     /locus_tag="ACG8H8_000740"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP8985832.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6450..7550)
                     /locus_tag="ACG8H8_000741"
                     /pseudo
     CDS             complement(6450..7550)
                     /locus_tag="ACG8H8_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7642..8529)
                     /locus_tag="ACG8H8_000742"
     CDS             complement(7642..8529)
                     /locus_tag="ACG8H8_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8985833.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8606..9151)
                     /gene="nusG"
                     /locus_tag="ACG8H8_000743"
     CDS             complement(8606..9151)
                     /gene="nusG"
                     /locus_tag="ACG8H8_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP8985834.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9252..9422)
                     /gene="secE"
                     /locus_tag="ACG8H8_000744"
     CDS             complement(9252..9422)
                     /gene="secE"
                     /locus_tag="ACG8H8_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP8985835.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9446..9598)
                     /gene="rpmG"
                     /locus_tag="ACG8H8_000745"
     CDS             complement(9446..9598)
                     /gene="rpmG"
                     /locus_tag="ACG8H8_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8985836.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9760..10404
                     /gene="cbpA"
                     /locus_tag="ACG8H8_000746"
     CDS             9760..10404
                     /gene="cbpA"
                     /locus_tag="ACG8H8_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP8985837.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10460..10963)
                     /locus_tag="ACG8H8_000747"
     CDS             complement(10460..10963)
                     /locus_tag="ACG8H8_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP8985838.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10976..11022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11560..12525
                     /locus_tag="ACG8H8_000748"
     CDS             11560..12525
                     /locus_tag="ACG8H8_000748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8985839.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12624..13796)
                     /locus_tag="ACG8H8_000749"
     CDS             complement(12624..13796)
                     /locus_tag="ACG8H8_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985840.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13783..14928)
                     /locus_tag="ACG8H8_000750"
     CDS             complement(13783..14928)
                     /locus_tag="ACG8H8_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985841.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14942..15874)
                     /locus_tag="ACG8H8_000751"
     CDS             complement(14942..15874)
                     /locus_tag="ACG8H8_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985842.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15991..17070
                     /locus_tag="ACG8H8_000752"
     CDS             15991..17070
                     /locus_tag="ACG8H8_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP8985843.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17067..17696
                     /locus_tag="ACG8H8_000753"
     CDS             17067..17696
                     /locus_tag="ACG8H8_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8985844.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17806..18033)
                     /locus_tag="ACG8H8_000754"
     CDS             complement(17806..18033)
                     /locus_tag="ACG8H8_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8985845.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18136..20724)
                     /locus_tag="ACG8H8_000755"
     CDS             complement(18136..20724)
                     /locus_tag="ACG8H8_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP8985846.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20744..21403)
                     /locus_tag="ACG8H8_000756"
     CDS             complement(20744..21403)
                     /locus_tag="ACG8H8_000756"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8985847.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21452..21955)
                     /gene="trmL"
                     /locus_tag="ACG8H8_000757"
     CDS             complement(21452..21955)
                     /gene="trmL"
                     /locus_tag="ACG8H8_000757"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP8985848.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22176..23042)
                     /locus_tag="ACG8H8_000758"
     CDS             complement(22176..23042)
                     /locus_tag="ACG8H8_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP8985849.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23151..23792
                     /locus_tag="ACG8H8_000759"
     CDS             23151..23792
                     /locus_tag="ACG8H8_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP8985850.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23854..25215)
                     /gene="mgtE"
                     /locus_tag="ACG8H8_000760"
     CDS             complement(23854..25215)
                     /gene="mgtE"
                     /locus_tag="ACG8H8_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP8985851.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25233..26132)
                     /locus_tag="ACG8H8_000761"
     CDS             complement(25233..26132)
                     /locus_tag="ACG8H8_000761"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8985852.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26134..26931)
                     /locus_tag="ACG8H8_000762"
     CDS             complement(26134..26931)
                     /locus_tag="ACG8H8_000762"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP8985853.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26909..27613)
                     /locus_tag="ACG8H8_000763"
     CDS             complement(26909..27613)
                     /locus_tag="ACG8H8_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP8985854.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27744..28319
                     /locus_tag="ACG8H8_000764"
     CDS             27744..28319
                     /locus_tag="ACG8H8_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP8985855.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28437..29114
                     /locus_tag="ACG8H8_000765"
     CDS             28437..29114
                     /locus_tag="ACG8H8_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP8985856.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8567 a         6170 c         5235 g         9144 t
ORIGIN      
        1 cgggtaaata cagtattatt tcggttgctt gctgcttcac ctagcccttg tactcgtgtt
       61 tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc taaaagcaaa
      121 atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg cataacaagt
      181 cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac gataggtgca
      241 gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt tccccaattg
      301 taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac
      361 atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac taaatgcggc
      421 aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac agaacgcggc
      481 attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa taaatagatc
      541 atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag caaaatcccc
      601 acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg tcccgctagt
      661 accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa
      721 gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat
      781 aacgctacca ttcgaatgat caattttgga cgatccacca cgtcccctaa aggaactaaa
      841 aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc gactgcactt
      901 gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta atacatattg
      961 gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt taaaggtgtt
     1021 ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac ttatggaaaa
     1081 gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt ttgttttcct
     1141 tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac agtaacactt
     1201 gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc accacacgca
     1261 tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg ctgtagaggc
     1321 aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg tatcatatag
     1381 cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac ccaatgattc
     1441 gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc agtgatgtta
     1501 ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa gctcaaacca
     1561 gcaatgactc caactactga agtaggaata cctggaacag cacacatcac cgctgtcaat
     1621 acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc
     1681 ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa atacaaccga
     1741 tagcccacta ccagtatcag aatgattccc aacgagatca acatcaatcg gattattttc
     1801 tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt aacataaaaa
     1861 caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa aaattattta
     1921 acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc ttcttcttta
     1981 gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc ttctttcaag
     2041 cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc tccagctgca
     2101 gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc agcagcagct
     2161 acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt aactaggtcg
     2221 ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat gttcaatgcc
     2281 attataattt cctccgttta attgttcttt tttatttaat gctttatgcg gcaattaagc
     2341 tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt tgcggactgg
     2401 cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca attttgccaa
     2461 tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt tgatttctaa
     2521 tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta catcttcgtt
     2581 actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt
     2641 tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt tagcatcacg
     2701 aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt caacgactac
     2761 tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg ctttttttgc
     2821 gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa gagttttttg
     2881 cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat tcatcaccta
     2941 ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc
     3001 tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa taaaaaactc
     3061 tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc tcggtaggaa
     3121 attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc tcaactagct
     3181 tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc aacattctaa
     3241 tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt gttgtgatcg
     3301 ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc aatacatcat
     3361 ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt ccgattggta
     3421 catggatatt tccagctttg tccacacggt aagttacttt accagctttc acttcgttta
     3481 ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt aagccttttg
     3541 gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt gcaacaacta
     3601 catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca cctacgaaat
     3661 ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact aaaacagttt
     3721 gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg tctgcttttt
     3781 tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt gcaatgtttg
     3841 tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa tcaacttttt
     3901 ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct tgattgtggt
     3961 tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa ctgagaagct
     4021 gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc agttccttca
     4081 accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt taattcagcg
     4141 atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg ttcgcctgaa
     4201 cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt ttttgtaacg
     4261 aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa acctgcttga
     4321 tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc cgcttgacct
     4381 agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg caatttaacg
     4441 attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg tggttaattg
     4501 gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga caaataatgt
     4561 gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa gatttttttc
     4621 tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa caacaatttt
     4681 tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca gatgaaagca
     4741 aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa aatgaggtgg
     4801 agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct gtgatgattg
     4861 gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata cgtagtatat
     4921 ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca aaaacctatc
     4981 gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa cccgatgatg
     5041 aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc ttgtttgttc
     5101 ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa aaaggaacac
     5161 gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt tctgaattga
     5221 atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc caccaagatg
     5281 ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg aatatcggca
     5341 ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt gaagtagatg
     5401 atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa
     5461 attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag
     5521 caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa atgagtggcc
     5581 gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa
     5641 tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt gatgcaaaaa
     5701 aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc ttaaaagctg
     5761 tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt tgtgctacac
     5821 cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga gatcggcttg
     5881 atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc gggttagtca
     5941 tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa gttggttctg
     6001 caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct catcaagcag
     6061 cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat cccgttgcag
     6121 gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa gcttttatct
     6181 ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg
     6241 caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca gaaggtggct
     6301 tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag agggcgtgat
     6361 aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa aacgaagaga
     6421 ggactaattg cccttctctt cgttttttat taatccatca atttcgtatt ttttacttca
     6481 tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt caggtgttca
     6541 aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac tggtcggcca
     6601 atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc tcgacggaca
     6661 ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt tggcaatact
     6721 tcaaaggacg caggacctcc atctaattga cgtccaccat gattggatac ccagatacca
     6781 tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc ttttacgata
     6841 actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt
     6901 gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc actttgttcg
     6961 ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat gacgtcttct
     7021 tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc ccctgcttct
     7081 actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata taattggaaa
     7141 aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg
     7201 ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc ttcccctttt
     7261 tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc taattcaatt
     7321 ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg tggcatgatt
     7381 ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc agaaccaccg
     7441 cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc tagttaggca
     7501 atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat agtagatcct
     7561 tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta agcaaacatt
     7621 tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa gctgatctac
     7681 ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta ctttttcctc
     7741 tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt ttaacggtac
     7801 tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa atcgagtggt
     7861 tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa cgacagcagg
     7921 cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat cattaaaagg
     7981 cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc catacgttcc
     8041 taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga ctttatctat
     8101 gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt gattccactc
     8161 gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt ttccagacaa
     8221 atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt cttttactgc
     8281 ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg tcccgctgta
     8341 gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg attctgccgt
     8401 tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac cacttaacgc
     8461 aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat ttttgattgg
     8521 ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa ggccaatcat
     8581 atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg aaatcaagtt
     8641 ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct ttttctccgt
     8701 caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt ttcacgactt
     8761 cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa cgcaagatat
     8821 gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga
     8881 atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc atgatcattt
     8941 caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc ccatttttga
     9001 cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc attttcatac
     9061 tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa tatgtatgca
     9121 agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat tgtaaaataa
     9181 aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa aagtacgttg
     9241 tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt atccattacg
     9301 taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc tttacggagt
     9361 tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt tagaaatttc
     9421 atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta tattgattac
     9481 aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca ctgactgatt
     9541 ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc tttgccattt
     9601 acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac gtaacttttt
     9661 acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga tatgattaat
     9721 ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa aacacttgta
     9781 tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga agcattaaaa
     9841 attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg taatattttc
     9901 cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg tgatatgacg
     9961 cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga tacttctttc
    10021 ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga cagtgaaaat
    10081 catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca atcttggagc
    10141 gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg agatttggct
    10201 gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac tcttgatatc
    10261 gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac tgaagcgaaa
    10321 gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt agaaattgaa
    10381 aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt
    10441 cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga tctaaataat
    10501 acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc gtgccatagg
    10561 ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt cccatgctga
    10621 tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact ttgttttgca
    10681 ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc acatccatga
    10741 taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc caaagcaatt
    10801 tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg atcggccctg
    10861 aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg atgccattta
    10921 caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct cctcttttct
    10981 cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca aacaataatt
    11041 ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag caaaagctta
    11101 aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg taaggggtca
    11161 taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg ttgtgaaact
    11221 taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa cggtctagca
    11281 agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac gtaagtaatg
    11341 gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt tcattttcct
    11401 agacttctgc tataataacc aagaattaat tttgtaacag atttttttga aaaaacaagt
    11461 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
    11521 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
    11581 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
    11641 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
    11701 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
    11761 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
    11821 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
    11881 tctggaaatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
    11941 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
    12001 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
    12061 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
    12121 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
    12181 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
    12241 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
    12301 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
    12361 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
    12421 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
    12481 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
    12541 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
    12601 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
    12661 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
    12721 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
    12781 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
    12841 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
    12901 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
    12961 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
    13021 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
    13081 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
    13141 gcactctggt ttcgtttact gatctctttg cgattaaacg ctagattgat gacgctgaaa
    13201 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
    13261 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
    13321 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
    13381 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
    13441 cgactctgta tcttgatcgt cgccatcttt cctacttcga cttgttcggt aaagtctgca
    13501 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
    13561 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
    13621 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
    13681 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
    13741 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
    13801 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
    13861 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc
    13921 gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata
    13981 cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat
    14041 caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc
    14101 taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc
    14161 accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa
    14221 aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat
    14281 caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac
    14341 cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt
    14401 gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt
    14461 catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat
    14521 acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc
    14581 cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt
    14641 tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt
    14701 gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt
    14761 gattacaatt ccagtattaa tcgtttcaag attgccacct tgatcaatct cgccaaaggc
    14821 cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt
    14881 atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc
    14941 tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt
    15001 tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca
    15061 atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat
    15121 cagaagctat tgttcgctga atcgctgaca acacattttc tgggagtaat gggatgtcga
    15181 tcgtcaaacg atccgccgtc cgtaccatat cttttagaga ttctttggtt cctttagcaa
    15241 tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag aatttctact tcttccatgt
    15301 aatgtgttgt atagacaatg gttgctcctt gtcgattcag ctccttgata ctttctaaaa
    15361 ttttattccg actttggggg tctacagcta ctgtcggctc atctaaaaaa attaaccgcg
    15421 gcttgtgggc aatcccacat gcgatgttca accgacgttt caagccacca ctaagttctc
    15481 ctggacgaaa cgaaacatat ttttctaatc ccactaattg aatcacctct tcaatcaagg
    15541 ctttcctttt ctctttttcg gaaacatata aaccgcaaaa ataatcgata ttttcttgca
    15601 cacttagttc gtcaaagacc gcaacgtctt gaggaacgac cccaatcatt gccttgacag
    15661 cataattttc aggagtcatt gttgtcccga acaatttaat ggttcctttg tcataattta
    15721 acaaagaaag catacagttg atggcagtcg atttcccact gccgtttgga cctaacagac
    15781 ctaagacttc tccttcttgt acttgcagat caaaatgatt caatgctaca aaatctccat
    15841 agcgtttgac taatttatca atttcgataa tcatcatgaa aaactccttc caaatttctt
    15901 gttgatctga ctataataaa gtgcaagaaa agaaactagt gacgattggc taaaccacac
    15961 atgacatttg tcacaatgaa ggagtctgct atgcataaat ctcggctact caacgaaact
    16021 agctttctac tggttatctc attgcttttg ctggtaagtc aaggattcgt tgctatgcaa
    16081 gttatctatt tattgatcgc cgttttattc actggtgtca gttatttact gcctacaaag
    16141 ccaaattgga ttttccaagt tattctattt cttttatgtg cctttttacc tgatcttgtc
    16201 ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag aaagccacct tgattacttg
    16261 tttttgttag gttttttgtt gatttcgacg gttggtaatc atccccccat caaggtcgcc
    16321 tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa aaaaagatca acgaacgacc
    16381 gatcttgaga aaaagtacat tgtctccaaa gatactcatt gggaaaaaca acaagagtta
    16441 tcggaacaaa ataaagcatt ggccctgtca caagaaacac aattagcctt gcaaattgtg
    16501 gaagaacgga accgaattgc ccgagacatc catgacaatg tcggtcatct gctgtcaagt
    16561 gcgattttac aaattggtgc gcttgaaatg atcaatcaag aaccaaaact aaaagaacca
    16621 ttagaaaatt taagtgccac cgttcacact gggatggatc ggattcgtca aagtgtccat
    16681 gacctccatg agacatcgat ctcttttcaa cactccttag agtttctgat cgctgatttt
    16741 ccttcttctg ttacgattga aggaaacttg tttgaaggat tgaatgaaac gcagcaaaac
    16801 tgcttcttaa tggtcatcaa agaagcctta accaacagta tcaaacatag tcatgccaaa
    16861 accgttgttt tacattttcg aacgttgccg gccttttatc gcttgcaaat cacgaatgat
    16921 ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt tgaaaacgat gcgtcaacgt
    16981 gttcaagaac aaaagggaca agtccatatt ttccaagaaa accagcaatt tcagttaacg
    17041 atcattttac caagggaggg acaccaatga taacagtagt cattattgat gatgatccat
    17101 ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt gtgttaggca
    17161 ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc gatgttttgc
    17221 tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa attttggcta
    17281 aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa tacatccgcg
    17341 aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa gcgattattc
    17401 cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc atcgagactt
    17461 tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct tcttttagtg
    17521 aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa gaaattgcag
    17581 atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta ctagaaaagc
    17641 tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg caataagtct
    17701 tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt ttttcacatc
    17761 ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag gattcgcagg
    17821 gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg ccttgtctaa
    17881 cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac tttcattcaa
    17941 tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt cttttgctaa
    18001 tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt ttgatttcag
    18061 cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat ttttagctat
    18121 ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag aactgttttc
    18181 cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa gaacgaaatc
    18241 tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg aagaaaacag
    18301 atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac tgtcatcttc
    18361 tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat cttcaaatgg
    18421 atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag tgatattcat
    18481 tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg attcattcgc
    18541 tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct tactgcgagt
    18601 cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga ccatgggcaa
    18661 aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat aagaaagagt
    18721 gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact ggataactaa
    18781 ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt ctcccatatc
    18841 tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt ctcctatccg
    18901 ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat tgaatatctc
    18961 ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg gtgctaatac
    19021 ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt tagagatcaa
    19081 gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat tctttgtgaa
    19141 atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac tagaaccatc
    19201 accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt ccaaaagatc
    19261 atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta ctaagataac
    19321 ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat cgaccataga
    19381 catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg aaggtgtttt
    19441 ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac ctgtcgtttc
    19501 gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa aaatttcttg
    19561 ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat tgacgattcc
    19621 gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata gaggggaacg
    19681 gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga atattttttc
    19741 gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt ttttacgact
    19801 gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac tgttttcgta
    19861 aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa ttacagtagc
    19921 aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga tcgtttgctg
    19981 aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct gaaaaatctg
    20041 atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga tttgttcggc
    20101 tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct ggtacggatt
    20161 ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga aagctaactg
    20221 actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt ggtttagccg
    20281 aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt gttttaatac
    20341 cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta caattttttc
    20401 tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta cgatcccatt
    20461 tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc cgtatttagg
    20521 atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat cgccgaaact
    20581 tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga tgtgtaccaa
    20641 aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg taccaacgaa
    20701 ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt aaaggttctg
    20761 gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca tatggcaata
    20821 atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat ccacccattt
    20881 cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt aaagaagctc
    20941 catgtcctac gaataaaagt gggccatctt tatgtcgggc aaccgcatct gctacaacag
    21001 tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa ggatcataca
    21061 gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac atttcatcga
    21121 tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta taaaaaatct
    21181 ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct cgctttgctg
    21241 tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat gctttgattt
    21301 cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa cgtctttcct
    21361 gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc aaactcctgt
    21421 ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt tcatagtgat
    21481 gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact atcattgcag
    21541 ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg cgtaaacact
    21601 tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt ccaaataaaa
    21661 agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg gcaaattttg
    21721 agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa ttactgtggt
    21781 aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt ttgtcatcag
    21841 ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca cacgtacggg
    21901 caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga ttcatcagga
    21961 tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt tgcataaaaa
    22021 aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag ctcttctcac
    22081 taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt gttgctaata
    22141 taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga aaaacgtttt
    22201 ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg attcttatat
    22261 aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc caaaagatct
    22321 ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc agttaatttc
    22381 attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt ttcaggttga
    22441 ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac caccttcaac
    22501 acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg cgaaaagata
    22561 tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc agctacacac
    22621 caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga aatatcaaat
    22681 ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct accttctccg
    22741 cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt ctcaataacg
    22801 ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc ttgatcgtat
    22861 tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag gtcaaaacgg
    22921 tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg gcaaaccgga
    22981 cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat tttttttaac
    23041 atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg caactgctaa
    23101 aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag atgatcaaag
    23161 aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga gccgatcgga
    23221 tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt gtgatcgaag
    23281 aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt cgtcctagag
    23341 ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat ttgattgaag
    23401 caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca ggctggctag
    23461 acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc accttccata
    23521 tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg ctggtagaac
    23581 atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt gtagaaaatt
    23641 tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt ttgcctggtg
    23701 gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta agcgaagtac
    23761 acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac atttgcaaca
    23821 ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca taaataaaga
    23881 agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga taaagggacc
    23941 gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta aactacctgc
    24001 aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc ccaagataaa
    24061 attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag ctcctgtgac
    24121 aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt tctcatcaga
    24181 aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac cggcagttcc
    24241 tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt cttcatagtg
    24301 actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc aaggcaaacg
    24361 tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc cggcaagtcc
    24421 tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga ctgtgacgat
    24481 tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat aatctctaaa
    24541 tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc gttcgctcat
    24601 cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag aaataacccc
    24661 tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag ctacatctgc
    24721 ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga tcgaaacaaa
    24781 ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt ccttgatttc
    24841 actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt tatccagcgt
    24901 attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag ctgcatagct
    24961 aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa tcacatcgaa
    25021 catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt tctcttctgt
    25081 aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac tgtttctgaa
    25141 tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt cctctaattc
    25201 ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct cacaactgcc
    25261 tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa tggatggtaa
    25321 aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat tcgcccgcca
    25381 tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct tatttgatgt
    25441 gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg cttctctatc
    25501 cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa tagcgaagaa
    25561 taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga aagccttcca
    25621 ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat atctaatttt
    25681 gcgtgcgcaa atccatgctt tgcaaaaagc atcaagccag atgtatcccg atctagtcgc
    25741 gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc ctttacacga
    25801 ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc tgctggttta
    25861 tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc atccaaaagt
    25921 acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc cttagctaga
    25981 gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg aaattttatt
    26041 ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac ttgctgagaa
    26101 tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa aggcatcttt
    26161 cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt cttctgctat
    26221 tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa ccgttacaag
    26281 ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa tcacgatcgg
    26341 agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca actgaaaggc
    26401 attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg tcgaaccagt
    26461 aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt ttttgatgta
    26521 tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca gtgctaaaaa
    26581 atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg gataactaac
    26641 actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt aatcccgcca
    26701 atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat cattcagaca
    26761 atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg agatgaccaa
    26821 ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt tcaaacgttc
    26881 tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca tcccctgcct
    26941 cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct gattttcctc
    27001 ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg gacatctctt
    27061 catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt tcttctggaa
    27121 aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa ttcatcgcaa
    27181 gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc tgtacaggat
    27241 actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg atgtaatctc
    27301 gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact tgatggatat
    27361 cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct tcttctaaac
    27421 gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact ggcttgactc
    27481 tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt ttgcggatcc
    27541 cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa aatgcttccc
    27601 aatctcgttc catagctgat acctctactt cattaacatt cattgtttat tctaccatat
    27661 atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa attaggcaaa
    27721 atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat aaatctatgc
    27781 tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat aaagatttcc
    27841 gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa caaaattgtg
    27901 gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca cctgctgaaa
    27961 aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca tttatcttcg
    28021 aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg aatatcgatc
    28081 ctgcttcatt aatccaaacc ggaaagctta caacaaaacg agctgaattc cctattgaag
    28141 aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt gaactggaac
    28201 tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat aaatttttta aaacgttttt
    28261 ctttacctta tcgtcctgct aagaacaaga tccaacgtat gcttgaagct aaaaattaga
    28321 aacctttcat tttcacaagg cacttgagtt cattttttta cttttttctg ttaagttata
    28381 ggatgtgaaa gaatctagtc actagttgta tagaatctat tttgggggag attcatatga
    28441 tcgaaatcta tttatttgtt aatcctttag gggaatactg tttcgaaatg gaacaacagt
    28501 tattacaatt tatcgaagaa gagtacggga caccatcaaa agaaaagatg caatttcgct
    28561 ttcttccttt ggtcaatttg cagacaattg gggatgtcat gcaacaaaaa ggaatttctc
    28621 agaacaatct tgaggaacgt aatcacttat tctcaatgac ttattctgca gcattggact
    28681 gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg ttttttgatc aaattgcaag
    28741 aagcagtcgg ctgtaatgac ataccatatt ctcaagaact tgctgaatcc atatttgaag
    28801 aaatcggcgg cgatttgaat atgtttaaag aagatcgtca gtctgacttc gttaaagaag
    28861 tattcttttc tgatcaaaaa attgctcggg aaatgggcat cacaaaacac ccttctgcag
    28921 tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt agtagaagta aaaggtacaa
    28981 gtagcctttg gagaatcaca gagctgtgta aaacaacaga taactcatat caagttttta
    29041 atggtgaaca tttacataca aatgattctc agttccattc aagaactagc catttacact
    29101 tgctttctaa ttaaaa
//
LOCUS       DAZKAO010000021        27959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000021 DAZKAO010000000
VERSION     DAZKAO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.021"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..973)
                     /locus_tag="ACG8H8_000766"
     CDS             complement(74..973)
                     /locus_tag="ACG8H8_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8985857.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(983..1339)
                     /locus_tag="ACG8H8_000767"
     CDS             complement(983..1339)
                     /locus_tag="ACG8H8_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8985858.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1694..1990
                     /locus_tag="ACG8H8_000768"
     CDS             1694..1990
                     /locus_tag="ACG8H8_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP8985859.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2634..3587)
                     /locus_tag="ACG8H8_000769"
     CDS             complement(2634..3587)
                     /locus_tag="ACG8H8_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8985860.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3610..4365)
                     /locus_tag="ACG8H8_000770"
     CDS             complement(3610..4365)
                     /locus_tag="ACG8H8_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985861.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4362..5321)
                     /locus_tag="ACG8H8_000771"
     CDS             complement(4362..5321)
                     /locus_tag="ACG8H8_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8985862.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5314..6264)
                     /locus_tag="ACG8H8_000772"
     CDS             complement(5314..6264)
                     /locus_tag="ACG8H8_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8985863.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6414..6866
                     /locus_tag="ACG8H8_000773"
     CDS             6414..6866
                     /locus_tag="ACG8H8_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP8985864.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6955..9222)
                     /gene="gshAB"
                     /locus_tag="ACG8H8_000774"
     CDS             complement(6955..9222)
                     /gene="gshAB"
                     /locus_tag="ACG8H8_000774"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP8985865.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9409..10407)
                     /gene="ftsY"
                     /locus_tag="ACG8H8_000775"
     CDS             complement(9409..10407)
                     /gene="ftsY"
                     /locus_tag="ACG8H8_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP8985866.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10418..11227)
                     /locus_tag="ACG8H8_000776"
     CDS             complement(10418..11227)
                     /locus_tag="ACG8H8_000776"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8985867.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11236..14817)
                     /gene="smc"
                     /locus_tag="ACG8H8_000777"
     CDS             complement(11236..14817)
                     /gene="smc"
                     /locus_tag="ACG8H8_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP8985868.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14837..15523)
                     /gene="rnc"
                     /locus_tag="ACG8H8_000778"
     CDS             complement(14837..15523)
                     /gene="rnc"
                     /locus_tag="ACG8H8_000778"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP8985869.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15871..17652)
                     /locus_tag="ACG8H8_000779"
     CDS             complement(15871..17652)
                     /locus_tag="ACG8H8_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8985870.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17678..18592)
                     /locus_tag="ACG8H8_000780"
     CDS             complement(17678..18592)
                     /locus_tag="ACG8H8_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985871.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18619..19581)
                     /gene="opp4B"
                     /locus_tag="ACG8H8_000781"
     CDS             complement(18619..19581)
                     /gene="opp4B"
                     /locus_tag="ACG8H8_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP8985872.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19581..20525)
                     /locus_tag="ACG8H8_000782"
     CDS             complement(19581..20525)
                     /locus_tag="ACG8H8_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985873.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20526..21530)
                     /locus_tag="ACG8H8_000783"
     CDS             complement(20526..21530)
                     /locus_tag="ACG8H8_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985874.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21798..22040)
                     /gene="acpP"
                     /locus_tag="ACG8H8_000784"
     CDS             complement(21798..22040)
                     /gene="acpP"
                     /locus_tag="ACG8H8_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP8985875.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22162..23163)
                     /gene="plsX"
                     /locus_tag="ACG8H8_000785"
     CDS             complement(22162..23163)
                     /gene="plsX"
                     /locus_tag="ACG8H8_000785"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP8985876.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23212..25248)
                     /gene="recG"
                     /locus_tag="ACG8H8_000786"
     CDS             complement(23212..25248)
                     /gene="recG"
                     /locus_tag="ACG8H8_000786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP8985877.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25365..27047)
                     /locus_tag="ACG8H8_000787"
     CDS             complement(25365..27047)
                     /locus_tag="ACG8H8_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP8985878.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27099..27461)
                     /locus_tag="ACG8H8_000788"
     CDS             complement(27099..27461)
                     /locus_tag="ACG8H8_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8985879.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27731..27919
                     /gene="rpmB"
                     /locus_tag="ACG8H8_000789"
     CDS             27731..27919
                     /gene="rpmB"
                     /locus_tag="ACG8H8_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP8985880.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7721 a         6053 c         4933 g         9252 t
ORIGIN      
        1 taaatggatc tactgctctt acttatcctt atgttttaca aggtattttc ttttacacat
       61 tactaatgct gtctcaatac ggaacgattg tcatcgcttg ttcaaattcc tcttgcaaat
      121 gttgtgtttg ataggcctga aaagctgggt gatgggcacg caagaggaca cgtaatttca
      181 taggatcata gctgtctagc aactgttctt tcaagacaaa atctcgggtc agctgccatt
      241 cataataata accaaaaggt ctgcagctgt aaatcgtgtg accgatggat ttatcttcaa
      301 agcgacgatg cgtatggcca aaaactactt gtttgatatt tgaataacgg tcaagtaatt
      361 ctccgaatga ttctgatcct aaaaatgcat ttaatttgtt ccaacgctca taaggagcag
      421 attgatacat gataaatgcc cgtttaggaa cgaaatgagt ggataaaatc acctcaagtt
      481 tttttgcttc cagctggtct agcaaatttt ttgcagctgc aagcatttct gcattgacct
      541 cgagatctgt tttcccacgc tggatgaacc gatcatacca atacaaacgc ttcattcgta
      601 cgattgcctc gtaatctttc aattcggaaa actgatagtc gtaccagcca ttcatcccta
      661 aaagaacggt ctgctcattt aaagcaatat accgttcatt taaaaaatgt gggtctggaa
      721 aatggtccat catctgttca ttgggcacat ctgccaactc gtgattgccg aaatgaaatg
      781 tcgtagataa tcctttattg acgaaaaatt cgacaacagc taggcaacgg tctactttat
      841 tagccgtatc tccggctaga tgaagccgag tgatcttgtg cttttgcaaa acttgccaaa
      901 gctgcatcag ctctgattcg ccgaatttat tgatgtccac atgtaggtcg ctgatgatcg
      961 ccaattttcc cattttatcc ctctagtctt ttcgaacaac ttgcaagttg ccttttctca
     1021 agccgcttgg tccttttaat tgtaaactat tataagttgg ttctaaagca agtgtggatg
     1081 gatcatccat gtaaagtatt gcactgtctt tgattttgat tggtccaact ggtgtatcag
     1141 ccactacaga ataaagactt agatctgttt gtgtatcttt tttgacaatg atcaatcgaa
     1201 atgccgtatc caattggcaa ctgacttggc tttctgcaaa tggaccgtca ccgttttcga
     1261 tatctaataa aagttgatcg tctgcttcta atttattctt caatacttct tgtgcttctt
     1321 ttgtcacttc taatttcatc tgtatcacct catgtcatag aataatcact ttattcatca
     1381 gatgcaacta atttgtctgg ttctgactgt tctcaattta ttggaaagta agtcattcgt
     1441 ttattatgat ttctcttatc acaaaagaga caactttccc ttttcttcgt tcagtttctt
     1501 gaacggaaaa agacccaaaa agcaacctgt tatttgttta accttattcc tttcttaaat
     1561 aacaaaaaat actataaaaa ataaaaaacc tttaacaact attggaaaaa gaattacttt
     1621 ttgttcacgg attattaaca actcttcggt agagtaaact tatcaaataa aagacttctt
     1681 tggaggaatc actatgaagg aatatataaa aaacatctat tttatcgaag aaacacaaaa
     1741 tatcgagggt tcttatattg aagtaaaaac actctttgtc aatgaagata aaaccaaagc
     1801 attggatatt tataaaaaat tagcttcaaa aaaaacgaat agttttggat tgatattaag
     1861 cgagtataaa atcaaagcag aagagagtta tttctatcaa ttactaaaac ggtggagtaa
     1921 gcttcccgcc gatttttatc gaaaaatgca gatcatcaac tatcaaccgc ttgctgagac
     1981 acatgcctaa atagaggttg tgaaatcagt gctttgagct aataccgaag gtcagctgat
     2041 tgaacattgt ttagaagaga ttgtgacaac tgcgatttgc tttgagtaat aagaacgctg
     2101 gacagaaaat agcttctcat attttctagt ctagtgggct tattgccgag aagcagcaga
     2161 tggaacatcg tttagaagag attgtgacaa ctgcgatttg ttttgagtaa taagaacgct
     2221 ggacagaaaa tagcttctca tattttctag tctagtggac ttattgccga gaagcagcag
     2281 atggaacatc gtttaaagaa gagatcggta caaaataaaa atatctaaat ttaagtttta
     2341 tctaagtttc gctttgtacc tttgattcaa atcaaaggag gcgatcttga tgctgaaaaa
     2401 atggatcaca ctattttttg ccactcccca ttctagaatc aacccgtatg atgtagataa
     2461 tgaattcacg ttgaaaaaag atttctcaaa taaataaaaa ccatccgtaa aatcatcaat
     2521 acgattttac ggatggtttt tgataacttc tttgaggata ggctcttgct atttaagaat
     2581 gtaagaaaca ttattgttac atagtatgag agtttaatcc tctgttattt atgctattta
     2641 aatgcttgat tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc cccgcttaaa
     2701 taccagacat ctggttcaag ggagatgatc tgctgatttt taccagcatt cgtttgtgct
     2761 accagttcat tggcagaaat atcatctttg ctgtcgtctc caccaatagc ttttgttctg
     2821 tctactacaa ataagatatc cggatttttt tccagcacat attcataaga aacactttga
     2881 ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa agccaaataa atcatggatg
     2941 aaaccgaatc tagaaccaga tccgtaggct gataattgcc cttcattcac aagtgtgact
     3001 agtgcttttt tgtcagtagc ttctgctttc tttttcgtct gatcgattgc actagataat
     3061 tcctctaatt gagttttagc ttcttcttgc ttaccaaaga tctcccctaa agcagtgaca
     3121 ttttgctgga aagattccca tggatttttc gcatctaaag aaaggaaaat ggtcggagca
     3181 atttgactta gatctttttg aaaatctctt tgtcggccag aaatgatgat tagatctggc
     3241 tgaagctgat taattttttc taagtctggt tcttttattc cacctgctga ttctactttt
     3301 ttataggatg aaagatactc aggaaggctg cttgtagctg ctccaactac cttatctcca
     3361 acaccaagag catccatcgt atctaaagaa ccattgtcga atacgacgac tttttctgga
     3421 ttatttggca cctcgatttg gtctccatta ctatctgtta tcgtcaatgt agtttcagtt
     3481 tgtgtgctgc ttttttttgt gtctgcctga ctggtgttgt tgctatttcc acatgctgct
     3541 aaaccaaatg atgctagaat caagatacct actactaatt ttttcatctc ttctctccta
     3601 atgttagttt tagaaataaa gacagaatct gcggccttca atctcacaaa tccgtatgtc
     3661 catttcgtat aattcatcca atacatcttt ttttatcatt tcttgggtcg ttccttgcgt
     3721 aaataactgt ccatctttca ttgcaacaat ttcgtctgca taacttgcag caaagttgat
     3781 atcatgaaga acaataacaa ttgttttccc gatttcttgt actaaatttt ttaaaatctg
     3841 catgatttga accgcaaaat tcatatctag attgttcaaa ggttcatcca gtaaaatata
     3901 atccgtgtct tgcgctagca ccattgcaat atacgcacgt tggagttgac cgcctgaaag
     3961 agtgtcaatc gattgatccg ctatttcttc taacccaagg tgttccagtg attcctgaac
     4021 gatttcctcg tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc caaattctac
     4081 taattctttc acggtgattt ggacatttat ttcattcgat tgtcgtagga tcgataattt
     4141 tttggctaat tcattggatt tccaagcctt tacttcgtta tggtctaaat agatttcacc
     4201 tgtatctttt ggtatcaaac gactgatcat cgacaacacc gttgactttc cagcaccatt
     4261 tggaccgata aaagcagtca acttcttttc aggaatcgtt aactgtacat ctttaaccac
     4321 tgattttcca tggtacagtt tattgacctt ttgtaaatca atcataggat actcttcctt
     4381 tcgcgaatca attttgtgac aaaataaatt cctccgacaa attcaacgac gacactcact
     4441 gttgtattcc agccgaaaat ctgctctact gcgagctgtc ctcctgtcag aaacagcata
     4501 gctaccagaa agcttccgat aaatagctga ccatgacgat aagtagaaaa gacacgataa
     4561 ctgatatttg cgacaataaa ccctaaaaaa gtaactggac ccacaagagc cgttgatgtc
     4621 cctgtcaaaa gactgacaca acataaaatc aaaaattcaa aacgatctgt gtctatcccg
     4681 aggtttactg cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg atatcgaaat
     4741 aacaggtacc agcagccgat aatgatcaaa gaagcaggca aaagatactg actctggata
     4801 ttgccaaagc ttgcaaataa tctgccttga agcaaatcat attcattcgg atccatgatg
     4861 acttgtaaaa aggtactgac actaccaaaa agcgtaccta aaatcatccc tgacatcagt
     4921 aataaaaaaa gattcgctct catttttttc aaaagaaaga aaaataacaa tagactcagc
     4981 ccgaccatca gcccaacgtt caataagaaa aaaactgtac ctgtctgctg aagcatcgtc
     5041 actcctccga taaagaagaa aaggaccgtt tggacaagga catataacga ctcaagtccc
     5101 aatactgaag gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat tgtggcactg
     5161 ctgacagcta gcgagacaag gataaatgca aaaattttct tactcctcaa tgcacatgca
     5221 aaatgccagt ttccatatgt atggatcgtc aagtacattc ctgtaacaaa taaaacaaga
     5281 acaagcatac caataaaaat agatggcttt tttttaagca tatcgtcgtc cccttatcag
     5341 aagataaagg aagatagccc caccgatgat tccaacgatc acactgactg gtatttcata
     5401 aggaaaaatc aacacgcgag cgacaagatc gcaaaatact aagaacaaac tacctgctaa
     5461 agcagttacg ccaattgttt tgtgcacttg atctccatat attctagaga caagattcgg
     5521 aatgatcact ccgataaaag gaatgcttcc taccatgatc aacatgactg cactagccaa
     5581 tgatactaag ccaatgccta aaaactgcat cttctggtaa tctattccca gatttactgc
     5641 aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa ccaagagcaa
     5701 caatggaaca acagcataca ataactcata atttcctcgt atcacagtcg caaaattccc
     5761 ttgaagccat gaagtcatat tttgtaccaa ctggaattga taagcaaaaa aagtagcaac
     5821 tgaaccaatg atattcccta acatgactcc aatcaacgga agcatcaatt gatttttctg
     5881 gaggaacaat cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa agcaaaatgc
     5941 tgtcaaggat cggacaatca aggatgcacc aggtaggaaa atcatagcca ctaaaatacc
     6001 taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa acaaatttat tttgggtcaa
     6061 atgttgcatg atcaacccac aaatactact ggtcgctccc acaaggatca aacaaatcgt
     6121 tcgtggcaaa cgagtcacta gcaagatttg taagctttgc ccatctaact ggagcagttc
     6181 tgacaattgt acattcgata ctccgatgaa caatgaaata ctacttaaaa taactaataa
     6241 tgctactagt cgtccgtagc gcatccaatc cacctttcta attgagaatg attttcattc
     6301 ttattttaag tgaatcgttg aacaaaagca accgcttttt catatgtttt aggtattatt
     6361 gggaatatgc tataatcgta agaaagacaa agagaagaag gagggtattt tttatggcac
     6421 aagatgatgt aaaaagccat ttggataagg gactttatgg tacaccttta gtgaaccctg
     6481 aagaacagca taaatattta gggacattca gggaacgctg ctatcttagt atgactgttg
     6541 ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa aaacatccag
     6601 atgcttccat actcttgaat ggggctctac ccctttctgt ccaaaaccaa tatatccagc
     6661 tagcaacgaa aaacaatagc cgtttcactg tagtcaacga ttacgtcact gcaaatcctg
     6721 aggaattcgg attgctctta acagcaaaag aagcagtaaa tgaacctgtg attgatatcg
     6781 aaaaaaaata cccagaagaa aactcttcgc cagctgaaaa agaaaaacca aaaaagaaaa
     6841 aaagcttctg ggatcgttta ttttaaaacg aaaaaacaca taaattttta catgcaaaaa
     6901 aagcttttga tcagattgac atcaaatagg acaatctgtc aaaagctttt tcttttaaaa
     6961 tacttcagga tataacaatt tcaatacatt catcgtcaat cttctgcctt ttcctgcaaa
     7021 aggataaaca tgcatatgca tggctggatt gaaatttgct tcgatgatcc catatgcttt
     7081 tttatcagtt gtaggatcga tttctttatc tggtataatc aaatcgatac cactaatctt
     7141 agctcctagt gcttctaccg cagaaacggc gatttttttg taagcttcag aaaattcatc
     7201 agtcatatcg atcgaatcac cacctgtact gatatttgag ttctccctta gataaacaat
     7261 ttgattggct tggggaactg attcgatcgt caacccttgt tctttcaaca tcaattgttc
     7321 taattcacct aattggatca attctaaagg tgcacgatga ttcgttccac gcaaagggtc
     7381 actatttttt tcttcaacaa gttctttcac tgttcggata ctgtcaccaa taacattagc
     7441 agggactcgc aacatgatgg cttggacttc tccgtcgatc acaaagaaac gatattccgt
     7501 tcccggcata aattcttcta ctaaaaccga actgtcttca cgaaaagcaa tggctaaacc
     7561 tgcttgataa tcttccagac tagctccttc cttgaaaatc gtgataccta acccgtaatt
     7621 ggttgattta ggtttgatca caaatgcctg ctcggcaaat ctcggataag cttttaccgc
     7681 ttcttccatg gatgaaaatt ctgcgcctcc tggtacccgg aacccagctt cttttaagac
     7741 ttttttcgtt actgttttgt tttccatgat aagtgggacg atatagctgt ccttacttgt
     7801 catgtttgca tttttcacat attctacatg gtcttggaat tgcagacgca aaaattgatc
     7861 cgactcatct aatactttga cttggacacc tttttggatg gcatcgaaca taaatatctg
     7921 agtagaaagt tccatttcgc gaaaaccggc caactgatag ggacgttcat ggctttcttt
     7981 ataatattga tgtcccaatg ccacagcaac ttcttgctga ctcgatagct gtgcttcttg
     8041 atagagtttc gctgctaacg tttgttcagg atgatccatt tgagtcaatg cttcctctac
     8101 taaataaaga gaatccaacc aatccaattc aaccagcatt tgcttcattc cttcaagaat
     8161 ttctctacct tcttgatgaa attctgtttg atctgatggt tgttctagtg ctactttgtt
     8221 atttcgagtc gtaccttctg ctacccattg gtcacagtct tctttttctt ctgtccataa
     8281 catgtacatc aggaataatt gtaagaaccg aacttgctct ggattgatac ccaatcttgc
     8341 ataaggattc aaatcgatat tccgcaattc aatataacga acacctgttg ttgctaaatc
     8401 tgcgactttc tttccgcctc ggaaacgaag cggtgtataa aattctttct cctccgaaag
     8461 tttcccttct tcgatcattc gctcgatatc tgctaaatac tgtttcatag aagcatagga
     8521 aactttcaca tttggatgat tcgtatagcc cagcgcactg ttgcgaatac tgcgaacagg
     8581 ttcttgcgga tgtgattcat ctaaaaagta gttcttctca ctcattggtg aagcaccgaa
     8641 taaataagtg atcatccagc gatagcgcat aaagtttctc gctgttttca aataaagttc
     8701 tgttttgaat tcggaaaaat cttgaaattc ttcctgatgg ctgaacaatg ttcttagcaa
     8761 ctcatcgcca aattcaaaat tgaaatgaat accgcttacc atctgttttc gcttcccgta
     8821 tttttttgct aaataacgtc gatacaagac atcttcgaaa ttttttaatt ttgcaataat
     8881 gatctcttcg tctttttccg gtaaggcagg tggcatactt aatggccaga tcatctcttc
     8941 tttcggtata gaacgagcag tcacatcata aacagcagcg agatactgaa ataattcggg
     9001 aatagaatcg gtaacagggg tgatcatctc tgtttgtgtt tcgctaaaat ctgtttggat
     9061 ataggggtga taggatcgat cgccaaagat tgctggatga tcggtttttg ctagatttcc
     9121 tgcaagatca acccgctgtc cttcccgttc tataccaaat cgagcttggt cgataaaagg
     9181 gcgcgcattg acatgcaata ataattgctt aaaattcatc attaagtgac ctccaattca
     9241 tatgaatcta attataatca aaaagtctaa gaaaaagaac gaaattgttt gagaaccgct
     9301 tacaaataag tataaaaatg aaggcatcaa aaacacagct atgttaaaaa caatcattca
     9361 aacaaggagc tgcaactttt gtcacagctc ctgcttttta gacatgggtt attcttcttt
     9421 tagtaatcct ttgaacaatc caacgacaaa atcatttgga tcaaacggct ccaagtcatc
     9481 gattccttca ccaagaccga ccaatttcac tggtaaatgc aattcattac ggatagcaag
     9541 tacgataccg ccctttgctg taccatctaa ttttgtcaaa acaagaccgg tcacatctgt
     9601 tgtttctttg aattgttttg cttgaaccat cgcgttttga ccagtggttg catctaatac
     9661 aagaagtgtt tcttggggtg cttctggtaa ttcccgctgg attactcgtt ttattttttc
     9721 taattcgttc atcaagttca ctttattttg cagtcggcct gcagtatcaa ctagtaaaac
     9781 atctgcctgc tctgtctttg ctctttccaa agcatcgaat acgacagctg ctggatcgcc
     9841 tcctgcattg ccacgaacaa cttcaacacc agcgcgttcg ccccagacaa ctaactggtc
     9901 gattgctcca gcgcggaagg tatctgctgc tgccatcaat acttttttac cttcgttttt
     9961 gaactgatgg gcaagtttac caatacttgt tgttttccct acaccattaa caccgacaaa
    10021 caaaataact gttagtccgt cttcttggat atttagttca tttacttcat tgattccttc
    10081 cgcttcatag agatccacaa gtttctcgat gatcgcattt tggacttctg ccgatttttt
    10141 cgcatttctg agtttcactt cttgacgtaa tgcttcggtg attcgtatcg ctgtttcaaa
    10201 acctacatct gcaccgataa gtgtttcttc taattcttca aaaaagtctt catcgactgt
    10261 tcgaaaattg gcaaacaact cattcatccg ttcaccaaat gttttacgcg tcttttctaa
    10321 acccttgtcg tacttctcct gtacttcaac ttcttctgtt ttctcttcgc cagtaaaagc
    10381 acgcttgatt ttatcaaaaa atcccatttg actcccccta ctctaataca aatttttgga
    10441 tcactttggc tacgccattt tcttcattgc tggctgtcac gaaatctgct gcttctttga
    10501 cgaaaggcac agcattctcc atcgctacgc ctagtccagc atattggatc atcggtaaat
    10561 cattttcctc atcaccgatc gccatgattt cttcttgttt caaaccaaga tcttttgcca
    10621 ataaagaaat accataagct tttgtaacac cttttggcat aaattccaaa agcatacttc
    10681 ttgttttgat gatctcatac atttcatgga attctgctgg tatttctttg attttaggat
    10741 ctaattcttc ttggtcataa gcgattactg ccttattaag cacaaattca tctgtaatat
    10801 cgcttatatc cgctggttgg aattgcagca gcttattcaa cacattgtac aaggattgct
    10861 tccttggggc actaggcagc tgtaaaacca cattgtcaga caaaacatct agtggcagat
    10921 ccaattgctg agccagtttg tataagcgtt ggatatctga tacttccatc actttctttt
    10981 ccatgatttc accagtatca tttttttgta ccaaaccgcc gttgaaagtg atgctgtagt
    11041 caccttcatc taccagtcct aattcttgca gataatgcgc catcgcagct aaggggcgtc
    11101 ctgtacatag aacgatcttg accccttgct gttttgcttg tgctaatgct tgtttattct
    11161 catctgaaat cagcttttgt tcatttaata atgtaccgtc caaatcgatt gctactaatt
    11221 tgatcatttt cgttcctaca ctttcgtttc gttgattttc gcctcttcat tgatatcttc
    11281 catccgtaca gaaacgattt ttgatacacc ggattcctgc atcgtcactc cgtacagtac
    11341 atctgctgcg accatcgttc ctttgcggtg tgtgactacg ataaattgtg tgtcatcttg
    11401 gaaatcgctc aagtaacgac caaaacgttt cacgttggct tcatctaacg ctgcttctac
    11461 ctcatctaag atacaaaacg gaacaggaca gacacggata atggaaaaga gcaacgcgat
    11521 ggctgtaagt gctcgttctc ctccagaaag caaacttaga ctttgcagtt ttttgccagg
    11581 aggttgtact tcaatctcaa tacctgtttt cagcaaatca gttggttccg tcagtactaa
    11641 ttcagcttta ccgccgccaa acatatttgg aaagacaatt ttgaattctt gacggatagc
    11701 ttcaaatact tctttgaatc ttgtccgcac ttcatcatcc atctcatcca tcgtttcaaa
    11761 caattggttt ttcgctgcta acagatcatc tcgctgtgtt gctaagaatg tatgtctttc
    11821 actgacttgt tcgtattgtt cgattgaatt taagtttact ggtcccagtt tttcaatctg
    11881 ttcttttaag gatgaaacct ttgttctact gtcttcgata tcggttgttt cttgatagtc
    11941 ttgggaagct ttctcaaatg tcagctgata ttctgactgc agataggtca aatgattatc
    12001 taaaatcatt tcagctcggt ctttttggac ttctaatttg gattgttccg acagtcgtgc
    12061 tttttgctca cgattacgct ctgccaagac tgcttcaagc ttatcgatct ccttttgcct
    12121 tctatctcgc tgctctttgg cttttgccaa ctcggctttt aatgtttctc gctgagcaga
    12181 taattcgtta atttgttttt ccaaactttc ttctgtgact tcatgatcag aaaagtctgc
    12241 tgtcaatgtc gccaactgct tttctatcgc ttcttgacgt tcggttgcct ctgctttttg
    12301 gactcgggca ccgcggagct ggatctgcag atgattaaac tgctctttca agacagcaag
    12361 atctgcttgt tcttgtgctt tttgactttg tacttgtgct cttctggctt ccatctggtc
    12421 gctttcttga cttaacgatt tgatttcttc gtcgattttt tgtcgttgcg attcaagatc
    12481 tgtttgtttg tctttcaatt cgttcgtttg cttttcgtaa tcttcgataa actgctgcag
    12541 ttcacgtgtc tcaaaagttg agatttgctt ttctttttca aaacgttcaa gttcatttat
    12601 gatgttttgt agctgatttg tcgcttcttg ttcttcaaaa cgtaattgtt cgcctcgtgt
    12661 acgcaaaact tcttgttctt cggctaaacg agctgtttct tgttgcagct cctgaacttt
    12721 tttttcttgt gcttggagtt gtttatctgc ttcttcaaat tcactagtca actgtttcaa
    12781 ttcttgattt tgtacaaaca aactgccttg gtttcctcgt ttattcgctc cacctgtcat
    12841 ggatcctccg gcattcatca catcaccttc taaggaaacc actcgatatt gatagcggat
    12901 cgtttgcgcg attgcattgg cactagttaa gtctttggct aaaaggatcg ttcctagcag
    12961 gttttgaacc acagtttgga tttgatcggg ataactgact tgttcactgg caatccctat
    13021 aaacccttct acagctgctg cttgtgtcaa tatatgagcc ggtaattgtc ttggtttgat
    13081 tgtcgtcagt ggaaggaatg ttgctcttcc tcctctttgc tgtttcaaat acgtgatggc
    13141 ttgccgagca tctttttcat tttcaacgat gacatgctgg gctgccccac ctaaagctgc
    13201 ttcgatagct aacgtgaaat ctgcaggaac atcgatcagt tcagccactg ctccgacaat
    13261 acccgaaagc tgctgtttat gctgcaagac caatcgaacc ccttggtaaa aaccaaaata
    13321 gttttcttgg atttcttgaa ggcttttctt acgagcacgt agctgctgga cttgattcat
    13381 taattgatac attttaggct gttcttttgc taatgcttct tggaactttt tcccgttttg
    13441 ttggatcatt tctaacttct cttttgtttc agttaaagat gattttagct gcgcctgctt
    13501 ttgtgtcaat tcttcttttt gtaagatcaa tcggtctact gatgcttcta cttccgattg
    13561 cttggctaac gtctgcttgc tttttgctgt ttcttgtata tattgacgtt ctagatactt
    13621 cagttcgttt cctacagctg cttgttcttg catcaaatca acatattgat ctcttaattc
    13681 tgccaataat tctttcgttg atttttggta tttttctaat tcttgctgtg tttttatgag
    13741 atttgcttca gctttttgga tctctgtttc tttttctgct gctgctttca tcaagctttc
    13801 ttgaagcttt tcaaaatgtt taaccttttt ttgtgcttct gctaatgaag tttgatattc
    13861 ttgcgagctt ttttgtgtat gctttgtccg ctcctgcaaa acatcttttt gtccttctgt
    13921 ctgcttcagt ttttccgaaa gatctaataa tacttgctga tttttttcga tcagacgatc
    13981 ggcttgcgca ttttctttac gctgcttcgc caaaatggat tcttgttctt ggatcgactc
    14041 agatagtttg cctaattcta gattgaattt cgcaagctgt gcttgtttgt tgtcccaatc
    14101 tttttttgct gtcttgattt cagcaaccat caaagagaca tcggtttgag tcagtgtttc
    14161 ttttagccgt aaaaactcct ttgctgcctc actctgggcg gccaaaggag ttaactgttc
    14221 ttccaattcg tggatgatgt cttggacgcg gctgaggttg tcttctgttt caaaaagctt
    14281 ctgttctgct ttctttttcc gctgcttgta tttcaagaca ccagctgctt cttcaaagat
    14341 cccacgacgg tcttccggtt tactactgaa aatcgcttct acttttcctt gagaaatgat
    14401 tgagaacgat tcttttccta atccagaatc taagaacaat tcttggatat cttttaatct
    14461 gcagctttgt ttattgatga aaaactcgct ttccccagtc cgtcgataac ggcgggttac
    14521 actgatctca ttgtactcca atggcagata atgatcacta ttgtctaaaa tcactgtgac
    14581 ttctgcaata ttcagctgct tacgggtatc ggaaccggca aagatgatat ccggcatttt
    14641 tccgcctcgc agacttttag cagattgttc tcctaatacc cagcggatag cctctgtaat
    14701 attgcttttt ccgctgccat ttggtccaac cactgctgtg acgctatttt caaaatcaat
    14761 gactgtcttg tctgcaaatg atttaaaacc ggctatttca attcgtttca aatacaaagc
    14821 tttccctcac ttacttttac agtgctgcca atgcattttc cgccgcagct tgttctgcta
    14881 attttttcga tttcccttgt ccagtgccaa tcaattgatc atctacataa acttcgatcc
    14941 aaaatactcg ttcatgagct ggaccttctt cattgatcaa tcggtattcg attgatacat
    15001 cacctgattt ttgcaagact tcttgtagtt ttgttttatg atccatctca tgtgaaaaag
    15061 cacctgcttt gactttagga aaaatcactt tttcgatgaa tgcatgggtc gtgtcgaatc
    15121 cctggtcaag atataacgcg cctaaaaaag cttcgaatag gtcgcaaagc aatgctggtc
    15181 gcgtacgtcc tcctgaattc tcttctccct tgcccaacaa aacatattga tcaaagtgac
    15241 attctttggc aaatttcgac aagctgtctt cgcgaacgat cgcagctcgt gtttttgtca
    15301 attttccttc tggcaaatga ggatataaac ggaataaata ctgagaaacc atcagttcta
    15361 aaacggcatc tcctaaaaat tcaagtcgct cattatccga taattgcaga ttgcgatgct
    15421 cattcacata ggatgaatgg gtaaaagctt gttcaagcaa atttagatcg tgaaaaacga
    15481 tgttatagtt ttcttttagt tcttttgtca gctggttgtc cattttccac attcctttta
    15541 tggatatttt ttcgatactt ccttattata ctggtttttt gtcaaatttg aaatggttcg
    15601 gtcaaaaggt aagtaaagct ttaataatcc tagtgattga cgagaagttg tgaaaaattc
    15661 gtttagtttc gagtatgaag aaaaaatttt gtgaaaatgg ctttccatat ttttgcataa
    15721 atttgactta ataccgagaa actggacttt gaacaccgtt tattggagaa gtcatgaaat
    15781 cagcgccttg acctgaatat tagagaaata aacagaaaaa tatcaaaaaa ccgaaaaagg
    15841 acatcgccct ttttcggtca tttttagtga ttatgaacgt gaatctgctg taacagaaac
    15901 atttgcccat gggtttggta catcataaga ccaatcccag tctttgacac ggtcgttgac
    15961 tggcaatact tcattacgga acaacgttgg aatcgcaaat gcttcttcaa atgcatattg
    16021 ttgccatttt tgatatgctt ctttttggta ctcttcatca aatgctttgc ttgaactgat
    16081 atcgttgatc aattttgtat tttcttctga ttcataacga gtgtagttga acgcagcatt
    16141 tggtccccat agtccacttg gatttggatc agttcctgta ttccaagcag cttggtaaac
    16201 atcaatttca ggatcatcgt tttctaattt atcatagaaa gaattgaatt cgatcagacg
    16261 acctgttgtg tattggacat ccaaaccaat ttctttccat tgttggatat agtaatctgc
    16321 caatggttgc gctgtttccc caccggacat cgaagcaaag ttgattgtta gtttatttcc
    16381 atctttgtct tctcttaaac catcgtcatc cacatctttg tagccagcat catctaatag
    16441 ttttttcgct ttgtctagat cttgtgtgaa tcctttcatc tcatcatcat ggtatgactg
    16501 gaatactgga ggaatcaatg aagtcgcatt gcttctcaaa cctgcataga aacgttcacc
    16561 tactgcatta ttatctaatg catagcccat tgcttgacgt aatgatttat cagccatttt
    16621 tgaatctgga ttgtattcca cactctttgt atctgcattc catttaccta acttgaatcc
    16681 tagataagta taagaagttt gttcacggcc taagtttgta tagccagcaa tttctttata
    16741 ggtatcgtaa gtatctgttg gcatttggaa aatcatatca tataatttag agttcaatgc
    16801 ttcagttgca cttgccatcg gtacagattt catcgtgatc tttttcaatt taggcgtacc
    16861 attccagtag tgctcgtttg gcaagtattc tactgattct cctgcaacga tattactcat
    16921 gtaataaggg ccaaaagtca ctggattttt acgtacttga tcgcttgatt ccatatcttt
    16981 gattgctaca ccttctaacg tatgttttgg tgctgcatat gaccagatac caccacccga
    17041 ttgaagcatg cttgggttta cttctttata ttcgatttca actgttttgt cgtctatttt
    17101 tttgattccg gagattgtgt cggcacttcc atcatgatat tcttccatac ctacgatgtt
    17161 acggaaagtg tcgtcataac ggataccagt gtaatcttta ttcccaatga tttcgtaagg
    17221 gaaaatcaca tcgtccgccg ttacatcttc accatctgac catttgacgt tgtcttttac
    17281 tttgattgtc gcttttttcg tatcttcatc tagatcaagc gttgcagctc cgtcgtctgt
    17341 gattctgaaa tcgtcatcgt ttgcaaatag cggttcatga gaaggagcca taaaagctgc
    17401 atcatatgaa tcttggtaaa actcccactg gaacaatcct ttaaactgac tgtccgttgc
    17461 gaccgcagct tctaattcgc cgtcttcgat tgcatcatca ttattttttg ttgcaattgg
    17521 aaaagttaat tcctctttgt tattgccgtt gcctccagag ttgtttccag aggtatctgt
    17581 acctccgccc ccgcatgctg caagaacgac agcagacatc agcgctgcaa atccaaaaat
    17641 cgttttcttc attcctattt cccccttcgc ttctatacta gcctaaccgt tggcgcgcat
    17701 ctgcagaacg cttgaatgct tgtccaacat aattgatact caacatcaaa actaaaataa
    17761 ggattgatgc gggtaaccag atccattgcc gattcactaa gacttctccg ttacttgcat
    17821 aaccgatcaa tgtacctaaa ctaggtacat ttgccggcaa gccgaaacct aagaaagtca
    17881 aagttgtctc gataccgatg tttgccgcaa agttcatcgt caaattagta atgattaatg
    17941 aacttagatt cggcatgatt tcgcggaaca tgattttaaa atcattggtt cccattgttt
    18001 ttgaggcact gacataatct cttcttgctt cagataatgt cttgcttcgg aataatcggg
    18061 ctttagctac ccaataaaat gcactcatga tgaatataaa ggaccagaca ttgtatctgg
    18121 aaataatcga tacgaagacg ataatgatca gcatgatcgg tagaatcatg ataaaatcaa
    18181 cgatccgcat caagatgttg tcaatgatcc ctccgtagta gccagagatg atccctaatc
    18241 caaccccgat gatcgaagta aggatcgtga tcgcaaatcc gatcgtaaca gagtttctag
    18301 cacctatgat caattgtcct aatacatcac gtccgccttc atctgctccc agaatgaact
    18361 tttgtccttg tgtcgaaact gttcctggtg cagcgtattt gtcgaagata ctaacataca
    18421 tcactttgtc ttgatcggtc agcatcgcac cgataaagat cactaataat aaaaccacta
    18481 gcaaacctaa tgaaaaaata gcaattttat cttttttaaa ttctcgagca atcattcgta
    18541 tccccatagg aggaatactt tcttgaactg ctgtattgtt tttttcactc atcgatccat
    18601 tctcctttct gattggtttt attgaatccg aatacgcggg tcaacgatac tcatgatgat
    18661 atctgaaagt aatgtcccca ctagcgtcgc gattcctaaa atcaatacta aggatgtgat
    18721 cacggtatag tcccgttggc cgatggaatc aataaatagt tttccgactc ccggataacc
    18781 aaaaatcttt tcgataacta cagaaccagc aatcaacgaa gtgatttcat accccatttg
    18841 agaagccatc ggcaacgctg catttcggaa aatatgtcga ctgaatactt tattcgtcgg
    18901 tacccctttg gaacgggctg ttctgacaaa atcgagcgat ttcgaatcga ttacttcact
    18961 tcgcagatat tggattgtga cggcagtcgc caaaaatgct tgggtaattg atggtaacag
    19021 aatatatttc aaacggtcta tccattgagt gataaatcca ccttgcaaac ctagtgtttg
    19081 tgatccactt gttgggaacc agcctaaacg ataaccaaaa acaaatagca tgatcaagcc
    19141 gaagataaat aaaggaacag caaaactaaa gaagttgtag attacgacaa tcttatccaa
    19201 gattgaattt tgataccggc cagcaagcat ccccagaggc acagcaatca aataagtaag
    19261 gacaactgtc aagatcgata gatataaagt gttgatgatc cgttcgccga tcaagtcagc
    19321 aacagagcgt ttgtataaga aactttgacc aaaatcgcct tgcaaagcat ttccgatcca
    19381 acggaagtac tggacatacc atgggtcatt caagccagct gcttgtcgca tctgttcgat
    19441 cactgccgga tcctgatttg gattgatcaa accggtaaat ggatcccccg gcatcgcttg
    19501 ggctaataag aagatcagaa cacttaaaat aataacttgc gggatcatca ataaaacacg
    19561 tcgcagaatc gttttccaca ttagcttgca cctccatctt tcaacgccac tttgtgtgta
    19621 gttgtgatcg tacgcaaatc gtagactcga ccagtcgcat catagtattc tttttggttt
    19681 tggatatact cttgttctac tcttcgtcga ttttctttgt ggctttccct attttctaca
    19741 tcgatttgcg gaatagctga aagcagacgt ttcgtataga tatgtcgcgg gtcattataa
    19801 atgtcttctc ttgtaccgat ttctacaaat cgacctttgt acataattgc gatattgtcg
    19861 cacatgtgtt ttactacacc taaatcatgt gaaatgaata aatagctgag gccgtattct
    19921 tgctggattc gtttcatgaa attcaatact tgcgcttgaa cagatagatc cagagcagat
    19981 acaggttcat cggctacgat cagttttgga ttggtagcaa cagcacgggc tactcctaga
    20041 cgttgacgtt gtccgcctga aaattcatga ggatatttat atagcgcatc ttccggcatc
    20101 ccaacgatat ctagcagtcc ttttaccttt ttcttttctt cttgatcgct taaccgttca
    20161 aaatttcgga tgggttcagc aatgatatcc aacactcgct ttttaggatt caaactggac
    20221 attgaatctt ggaagatcat ctgcacatcc ttgttataac ccattttttt gcggttgctt
    20281 cgcttcgtta catcttttcc ttcatacatg atttgtccgg ctgttacctt ttctaatccc
    20341 acaacggctt ttcccgtagt agattttcca gagccagact ctcccactaa accatatgtc
    20401 tttccttttt cgataataaa atccacacca tctacagcat atacatgatc cgttactcga
    20461 ttgaagaatc cactgcgaat agggtaatga actttcaggt ctttgattgt gattaattct
    20521 gtcatctaca cctctccttt ttcgtctcta aagtggaaat gcttatagca ggagcaacgt
    20581 accaagtggt tcggtgcgac ttcatgaagg ataggctctt cttcatgatc gcttgctggt
    20641 atccatggaa tacgtggtgc aaaccgacaa cctttacgag gcatgttttt cagagaagga
    20701 acgacacctt cgatcacatg gagttctgca tcatgatttc cttcttgtgg tatagagttc
    20761 agtaaagagc gtgtataagg atgcttagga tgatcaaaca gttcttttac acctgctact
    20821 tcaacgaact gtccgccata catcactgcc actcgatcag ccatttctgc tacgactcct
    20881 aagtcatgag tgatcaagat gatccctgtt tttgtttctt cctgaaggtc tttcagtaaa
    20941 tcaagaatct gtgcttggat ggttacatct agggctgtcg tcggttcatc tgcaatgacg
    21001 attggaggtt tgcaggcgat tgcgattgcg atgatgacac gttgtctcat accgcctgat
    21061 agttcatggg gatattggcg tccaactctc gcaggattag gaatccctac ttgttctagc
    21121 aattctagtg cacgagcttg acgctgttct gcattcatct ttgtgtgata ggaaagtcct
    21181 tctttgatct ggtcttcgat tctcatgaga ggatttaatg cagacaacgg atcttggaaa
    21241 atcattccga tatcatttcc gcggatttta ttatagagcg tttcgttcag gtcgattaaa
    21301 ttcagatcgt tatataaaat ctcacctgta atttttgtgt tattaggatc aagtaatccc
    21361 ataatcgtag tagccaaggt acttttcccg cacccagatt ccccaacgat tgctaaaatc
    21421 tcgtttttgt ctaaagtaaa tgaaacgtca tctactgcat cgtaatagtc atctttgagt
    21481 cggaatcctg tatgtaggtt ctgaacttct aataattggt tatcggacaa tcgaataccc
    21541 ctctctgctt cctaaatgga agtttttctt aataaaaaag gacattacac agaataaagc
    21601 gttttataac aaaggttatt cttatctttg tgtaatgaat tattaataat tatatagaaa
    21661 atcgctttac tttgcaacat aatttctgtt tatcgccaga attatcagaa aaatgacttt
    21721 tacgtgtcaa aaagattaaa aaaagaagga taaaaagctg tatgcagctt tttatccttc
    21781 tttgctttta tttgaaatta tttaagatgg ctgctgatat aatctactgc gccaccgact
    21841 gtttcaattt gttcagcgtc ttcatctgaa atttccgtac cgaattcatc ttcaagctct
    21901 aaaacgaatt ccataatact aattgaatca gcttttaaat catctttgat gttcaattca
    21961 tccgtcactt tatcggtatc tatctcaaaa tgatttgaaa taattttcgc tactttgtta
    22021 aatacttctt cgcgagtcaa ctttttcacc tccaagaatc atacacttgt caattaccta
    22081 caccatggct aattttactg gcatttttca ggaatgtcca cctttttttc aatggaacgg
    22141 attgcttcag atatccaaat actattcttc gcttttttca aattgttcga ctaattgtcc
    22201 aactacatct gtctcaagca ttgtgtggat ttggcggatc gtatagcgaa cagcatcggg
    22261 gcctgttgct ccatgagttt taatgactgg tgctttcaag ccaaacaaaa cggcaccgcc
    22321 atgtttggaa taatccattt ctgctttcaa agaacgtaag ccgtctttta aaagcatagc
    22381 gcccattttt cctttgatcc cttcatttag aatagcagat tttaatagat tcatcatatt
    22441 cattgctgtt ccttcaatag atttcaaaac agcgttgcct gtaaatccat ctgtgaccac
    22501 tacatcagcc acaccattga gtagctcacg agcttctacg tttccaataa aattcaagct
    22561 tgtttcattc tgaaggagtt cataggcttt tttcgtcact tcactacctt ttgttgcttc
    22621 tgttccgttg tttaagagtg cgacacgagg acgtccgata cctcggactt tacgtgcata
    22681 aaaagaacct aaaataccgt attgcaacaa atgttctggt ttgttttctg cgttagcgcc
    22741 aagatccagc atatcgaaac cggcaccttc tttccctact actggcaagg tagacatcaa
    22801 gccagggcgt tcgatatttt tgatacgtcc tacgatgaac aaccctgctg ctagtagcgc
    22861 gcctgtattc cctgctgaaa atacagcatc tgcttcaccg tttttcactg cttgtgcagc
    22921 aaggaccatt gatgcagtct tttttcgtcg aattgctttt actggctcat catcactatt
    22981 gattttttcg tctgtatgga taatcgtgat gtttttttca tttgttaagt atttcttgat
    23041 ttctgcttct tttccgtaaa gtaagaattc tacttctgga aaatcttgct gcgctaaagc
    23101 cacgccttct acaattgctt gaggtgcgtg atctccgccc attgcatcta cggcaatttt
    23161 cattgtttcc actccttttt ccgtcatcag tataaagtat agcacagtct gctaatcgaa
    23221 ataacgatta tcctgatttt ctatttggag gctttgcgct aaaggctgat actcaggaag
    23281 catctgccaa tcctttatct gccaaagggc tgcagcctct tctctggcta cctccaaaac
    23341 atttccatcc gtcaccaagt cagcaatcgc aaattgaggc agaccagatt gacggaagcc
    23401 aaatacttca ccaggaccac gtaattccag atctttttcg cttagtacaa aaccattggt
    23461 ggtctctgtc atgattttca tccgttccac acctagttca tttttaggat tagcaactaa
    23521 gatacagtag gaagcatcac ttccgcgtcc aacacgtcca cgaagttgat gcagctgtgc
    23581 caatccaaat cgatccgcat ccatgatcag catgacggtc gcgttcggca cgtttacccc
    23641 tacttcaatt acagtagtcg aaactaatat ctgcatctgg ttgtctttga aggcttccat
    23701 gattacttct ttttcctgat tcttcatttt accatgtaac aagccaactt catattttgg
    23761 tgcaaatagt tcacgaagtt tttcatagat ttccactgca ttttttacat ctaatgcttc
    23821 tgattcttca atcagcggac agatgacata catttgatgt cctcttgcca gttctttttt
    23881 cgtccaatcc agtacactgt ccaattgagg tgttcttacc cagcgtgttt cgatcgggat
    23941 acgtccagct ggaagttcat caatgataga tacatccatc tcaccatatg ctgtaatagc
    24001 taatgttcta ggaatcggag tcgctgtcat aaataagaca tctggtcgcc agcctttttc
    24061 acgaagtact ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa taactagacc
    24121 taaattctga aattcgactc catcttggat caatgcatgt gtgccgatca cgatgttaat
    24181 ttccccttgc tctagttttt caaggagctc acgacgttct tttgctttcg ttgaccccgt
    24241 aagcaatgcg atggttactt cattgggatc aaacagctgt tgcaagcttt ccatatgttg
    24301 ttgtgccaag atttctgtag gcaccatcaa cgcaccttgg aaacctgctg taacggtagc
    24361 gtataacgca atcgcggcaa ccactgtttt tccgctacct acatctccct gcaaaagtcg
    24421 ctgcatatgc tgcggactgc gaagatcccg acagatttca ttcgttacac gtttttgtgc
    24481 atttgtcagt tcaaaaggta gcttttgagt aaattctttc aatttttgga catcataaag
    24541 gatctccaaa ccattcgttt ctgctttttc tgcttttttc aatccttgaa gacgcatttg
    24601 aaataaaaag aactcttcaa aaacgacacg tcgtttggct tgatggtttt cttcgtggtc
    24661 ttttggaaaa tgcatacttc ttacagcaga agctcggtca agcagtcgat atttctccac
    24721 aaggttgttt ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc cttttttgat
    24781 tagatcaact aatgtcgttt gacgaattga tttgttgacg tgatatatcg gcgaaaagtc
    24841 atccattgtt tgagaaccta aaatcttcat cccgttcaat gctttacgct ttgcatccca
    24901 tttaccgtat acggcgattt cctccgataa aatgactttt tcttttagat aaggttgatt
    24961 aaaaaaagaa acgttgaaca catcatgttc ttgcatcata cgaaattgca atcggctttt
    25021 tttataacca aatcgattca tcacaggagg cgagactacg attcctttca aagtaacctt
    25081 ttcttgatct tggatctcgt ttagatttct ttcctgaata tcttcgtagc ggaaaggata
    25141 ataagtcagt aagtcttcaa tcgtttggat gcccaagctg gcaagactag ctgctctttt
    25201 ttctccgact cctgccagca tagtgattgg atcatctaaa gaacgcataa atttttcctc
    25261 cctcccttaa caaaaaaagg acttggtcag cgatcatacc tgatcactaa ccgaaggcct
    25321 tttatgaatg aatactgtgt tagaaagata ttctgacaca gaaattattc agcagaaaat
    25381 agataaggat agacaggctg atctccttgg tggatctcga tttccaaatc aggatgtttt
    25441 tctgttagtt tctcagcaaa ttcatttgct tgttcaactg ttccttcatc acctgccaag
    25501 atcgttacga tctctgtgtc ctcattgatc atttgttcta gtgtacttaa cgctgttgct
    25561 gtgatatctg ggttagagat caggattttt ccatcgatca tgcccaaaaa gtctccttct
    25621 ttgatctcta caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg accgctcaca
    25681 acgctagaca acatttctgt catcgtttct ttgttttctt ctaaagaagc ttgatcattg
    25741 aatgaaagca tagctgtcaa tccttgggaa atcgttcttg aaggaacaac tgctactggg
    25801 atatctgcta cttctgctgc ttgatcagct gccatgaaga tgttcttatt gtttggcaag
    25861 acaatcactt tatctgcgtt tacttcttga atcgctttaa caatgtcttc cgtacttgga
    25921 ttcattgttt gcccaccact gataacataa ttagcgccta aactttcaaa cagctcttgg
    25981 acgcctttac cagcagcgat cgctacgata ccatacggga tacgtggtgt ttcttttttc
    26041 gcaaactcgt tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa gcgcatgttg
    26101 tcgactttga ttttgactaa tgaaccgaat ttttgtccat aattcaagac ttctcctggg
    26161 ttttctgtat gcacgtggac tttgatgatc tcgtcatcat tgaccacaag taaagaatct
    26221 cctataccat ctaaatagtt acggaattct tcgtaatcga atgtactatc gactgtaggt
    26281 ccttctccga tttttaccat gatttctgta cagtaaccaa aatggatatc ttctgtcgcc
    26341 atttgacctt ggacactgcg atgatgttct gcattgacca tatcatccat ttctgctgga
    26401 gaaggttcat atgtttcatc cgcttggaat tctccattca atgcacttaa aaagccctca
    26461 taaacaaaca gcagtccttg ccctccactg tctacgacac cgacttcttt taggactgga
    26521 agcaagtctg gagttttagc taaagcacgc tttgcccctt ttacaacagc tgtcattact
    26581 tcaacgacat catcagtact ttttgctttc ttttctccag ctttcgctgc ttctctagct
    26641 actgtcaaaa tcgttccttc tactggtttc attactgctt tatacgcagt ttcaactcca
    26701 tgagtgaagg ctgcagcaag atcggaagca tttaatgaga caacatctgg aatttgctta
    26761 gaaaatccac gaaacaattg agacaaaata acgccggaat ttccgcgcgc acccatcagc
    26821 aatccttttg aaagcattgc tgctaattct cctacttttt ctgaggttga atctactact
    26881 gccttagctc cactagtcat agatagattc atatttgttc ccgtatcgcc atctggcaca
    26941 gggaatacat ttaatgaatt gacgtattct gcatttactt gcagacgatt cgcacctgcc
    27001 tggaccattt cctggaactg gcttgcactt atttctgtta cactcaccta gataatcctc
    27061 cttcagatcg actgctgagt taccagccag tctcctgcct agtctggcag tacacgaaca
    27121 ccttgtacaa agacattaac tgaattggca gttacgccta ataatgcctc tagattgtat
    27181 ttcacttttt cttggacgtt gcgggaaact tcagagattt tcgtaccata gctgataatc
    27241 gtataaacat ctacagcaat accattatct tcttggcgga caactactcc tcttgagtaa
    27301 ttttctttac gcaaaatctc agtaatatta tctttaattt gatttttgct ggccattcct
    27361 acaacaccga acacatctgt cgcggcgcca ccaacaactg ttgcaattac atcattggta
    27421 atttcaatgg ttccagctga cgtttgaatt tttacagcca tgaatgagcc tccttaacgg
    27481 gtaaaaaact cccatttttc ttctatcata ttttatcata gcgcaacttg aaataaaagg
    27541 cagttaccca aagtactcaa aaaccttgca aaatttcagg tataaccttg tttaatttac
    27601 aattattctt ctcaattgtc aattgtatct atgaaaatgt acttgcaaat ttccattcta
    27661 tatgatatgt tagtctggta tgagctgaat tattctattg ctccaaatca aggcaaagga
    27721 ggaaattacc atggcaaaag tttgttactt tacaggtcgc aagacaaaaa gtggaaacaa
    27781 ccgttcacac gctatgaact caacaaaacg tactgttaaa cctaacttac aaaaagttcg
    27841 cgttatggtc gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt tgaaatcagg
    27901 aaaagttgaa cgcgtttaat tgaatgatac ttggaccgtt cagatagtga acggttttt
//
LOCUS       DAZKAO010000022        27691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000022 DAZKAO010000000
VERSION     DAZKAO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.022"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1169)
                     /locus_tag="ACG8H8_000790"
     CDS             complement(225..1169)
                     /locus_tag="ACG8H8_000790"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP8985881.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1465..2409)
                     /locus_tag="ACG8H8_000791"
     CDS             complement(1465..2409)
                     /locus_tag="ACG8H8_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP8985882.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2630..3844
                     /locus_tag="ACG8H8_000792"
     CDS             2630..3844
                     /locus_tag="ACG8H8_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8985883.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3866..5881
                     /locus_tag="ACG8H8_000793"
     CDS             3866..5881
                     /locus_tag="ACG8H8_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP8985884.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5964..7337)
                     /locus_tag="ACG8H8_000794"
     CDS             complement(5964..7337)
                     /locus_tag="ACG8H8_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP8985885.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7363..7647)
                     /locus_tag="ACG8H8_000795"
     CDS             complement(7363..7647)
                     /locus_tag="ACG8H8_000795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8985886.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7649..9664)
                     /locus_tag="ACG8H8_000796"
     CDS             complement(7649..9664)
                     /locus_tag="ACG8H8_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8985887.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9843..10148)
                     /locus_tag="ACG8H8_000797"
     CDS             complement(9843..10148)
                     /locus_tag="ACG8H8_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP8985888.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10237..11268)
                     /locus_tag="ACG8H8_000798"
     CDS             complement(10237..11268)
                     /locus_tag="ACG8H8_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8985889.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11475..12350
                     /locus_tag="ACG8H8_000799"
     CDS             11475..12350
                     /locus_tag="ACG8H8_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8985890.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12502..13344)
                     /locus_tag="ACG8H8_000800"
     CDS             complement(12502..13344)
                     /locus_tag="ACG8H8_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8985891.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13436..15313)
                     /locus_tag="ACG8H8_000801"
     CDS             complement(13436..15313)
                     /locus_tag="ACG8H8_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8985892.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15310..16731)
                     /locus_tag="ACG8H8_000802"
     CDS             complement(15310..16731)
                     /locus_tag="ACG8H8_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8985893.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16734..20123)
                     /locus_tag="ACG8H8_000803"
     CDS             complement(16734..20123)
                     /locus_tag="ACG8H8_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8985894.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20380..21771
                     /locus_tag="ACG8H8_000804"
     CDS             20380..21771
                     /locus_tag="ACG8H8_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985895.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21844..22491)
                     /locus_tag="ACG8H8_000805"
     CDS             complement(21844..22491)
                     /locus_tag="ACG8H8_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8985896.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22499..23185)
                     /locus_tag="ACG8H8_000806"
     CDS             complement(22499..23185)
                     /locus_tag="ACG8H8_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985897.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23191..24264)
                     /locus_tag="ACG8H8_000807"
     CDS             complement(23191..24264)
                     /locus_tag="ACG8H8_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985898.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24486..25160
                     /locus_tag="ACG8H8_000808"
     CDS             24486..25160
                     /locus_tag="ACG8H8_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8985899.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25165..26505
                     /locus_tag="ACG8H8_000809"
     CDS             25165..26505
                     /locus_tag="ACG8H8_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP8985900.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26654..27640)
                     /locus_tag="ACG8H8_000810"
     CDS             complement(26654..27640)
                     /locus_tag="ACG8H8_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP8985901.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8070 a         5239 c         5028 g         9354 t
ORIGIN      
        1 cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca
       61 acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac
      121 cattctattt atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca
      181 gctgttaagc agttttagga tttttagttc atattcattg tttcttataa tacagtgttc
      241 aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt
      301 gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag
      361 atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg
      421 ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct
      481 tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat
      541 tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca
      601 gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg
      661 acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc
      721 aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata
      781 tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc
      841 atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga
      901 gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat
      961 tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa
     1021 aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta
     1081 gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg
     1141 attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt
     1201 tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt
     1261 tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa
     1321 tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa
     1381 aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat
     1441 atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt
     1501 tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc
     1561 gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt
     1621 ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata
     1681 gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt
     1741 atcgatataa tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa
     1801 gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa
     1861 cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg
     1921 cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc
     1981 ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa
     2041 tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc
     2101 tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat
     2161 ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc
     2221 aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat
     2281 atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc
     2341 taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc
     2401 gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc
     2461 ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt
     2521 tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata
     2581 accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa
     2641 taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc
     2701 agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt
     2761 tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt
     2821 tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat
     2881 ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg
     2941 actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta
     3001 tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc
     3061 agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt
     3121 agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
     3181 aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc
     3241 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
     3301 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
     3361 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
     3421 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
     3481 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
     3541 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta
     3601 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
     3661 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
     3721 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
     3781 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
     3841 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
     3901 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
     3961 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
     4021 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
     4081 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
     4141 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
     4201 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
     4261 tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa
     4321 agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg
     4381 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac
     4441 tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct actataagca
     4501 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
     4561 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
     4621 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
     4681 agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
     4741 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
     4801 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
     4861 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
     4921 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
     4981 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
     5041 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
     5101 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
     5161 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
     5221 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
     5281 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
     5341 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
     5401 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
     5461 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
     5521 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
     5581 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
     5641 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
     5701 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
     5761 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
     5821 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
     5881 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
     5941 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
     6001 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
     6061 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
     6121 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
     6181 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
     6241 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
     6301 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
     6361 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
     6421 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
     6481 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
     6541 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
     6601 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
     6661 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
     6721 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
     6781 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
     6841 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
     6901 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
     6961 gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg
     7021 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
     7081 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
     7141 gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg
     7201 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
     7261 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
     7321 ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat
     7381 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
     7441 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
     7501 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
     7561 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
     7621 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
     7681 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
     7741 ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga
     7801 acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca
     7861 aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat
     7921 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
     7981 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
     8041 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
     8101 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
     8161 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
     8221 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
     8281 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
     8341 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
     8401 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
     8461 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
     8521 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
     8581 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
     8641 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
     8701 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
     8761 tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga
     8821 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
     8881 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
     8941 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
     9001 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
     9061 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
     9121 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
     9181 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
     9241 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
     9301 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
     9361 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
     9421 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
     9481 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca
     9541 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
     9601 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
     9661 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
     9721 tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa
     9781 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
     9841 gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
     9901 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
     9961 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
    10021 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
    10081 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    10141 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    10201 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    10261 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    10321 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    10381 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    10441 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    10501 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    10561 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    10621 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    10681 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    10741 atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg
    10801 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    10861 gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    10921 ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    10981 ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata
    11041 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    11101 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    11161 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    11221 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    11281 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    11341 ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa
    11401 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    11461 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    11521 cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    11581 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    11641 aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat
    11701 tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca
    11761 ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat
    11821 tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa
    11881 tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg
    11941 tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag
    12001 ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt
    12061 atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa
    12121 atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat
    12181 atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata
    12241 cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca
    12301 aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc
    12361 tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca
    12421 ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata
    12481 tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag
    12541 atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg
    12601 tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg
    12661 gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt
    12721 cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt
    12781 gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc
    12841 taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga
    12901 aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc
    12961 acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc
    13021 agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg
    13081 ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg
    13141 gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc
    13201 ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac
    13261 aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag
    13321 aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa
    13381 gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa
    13441 tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg
    13501 caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat
    13561 ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag
    13621 taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat
    13681 agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa
    13741 ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata
    13801 aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt
    13861 ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga
    13921 ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc
    13981 ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc
    14041 caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct
    14101 cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat
    14161 acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga
    14221 atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag
    14281 aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg
    14341 ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg
    14401 ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt
    14461 ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt
    14521 caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat
    14581 gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt
    14641 attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg
    14701 cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta cagactgtat
    14761 ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg
    14821 cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc
    14881 cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta
    14941 aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag
    15001 tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat
    15061 aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat
    15121 cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt
    15181 tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga
    15241 agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt
    15301 tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct
    15361 tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag
    15421 aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc
    15481 ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga
    15541 aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc
    15601 gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc
    15661 gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc
    15721 taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac
    15781 agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc
    15841 cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc
    15901 agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg
    15961 tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa
    16021 aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt
    16081 tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct
    16141 aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg
    16201 atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac
    16261 catattctct gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc
    16321 tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt
    16381 gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc
    16441 gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc
    16501 tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc
    16561 tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt
    16621 gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg
    16681 tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca tatttagctg
    16741 ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct
    16801 actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga
    16861 ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga
    16921 tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt
    16981 agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa
    17041 ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct
    17101 ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt
    17161 accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc
    17221 atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa
    17281 tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca
    17341 ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc
    17401 tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc
    17461 tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga
    17521 ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt
    17581 accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct
    17641 tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct
    17701 ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt
    17761 aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc
    17821 aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa
    17881 tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta
    17941 ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg
    18001 tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata
    18061 tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact
    18121 tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga
    18181 ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg
    18241 tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca
    18301 ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag
    18361 atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga
    18421 cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt
    18481 ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg
    18541 tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct
    18601 gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga
    18661 tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc
    18721 gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca
    18781 ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa
    18841 aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa
    18901 aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat
    18961 agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa
    19021 ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg
    19081 ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg
    19141 ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc
    19201 cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg
    19261 atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt
    19321 gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg
    19381 atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc atcccattga
    19441 tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa
    19501 ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta
    19561 gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt
    19621 tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta
    19681 tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg
    19741 tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta
    19801 gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct
    19861 ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc
    19921 ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga
    19981 tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct
    20041 attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc
    20101 agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg
    20161 aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata
    20221 attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt
    20281 tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa
    20341 aaacatatga tcaggttgtt ggaatgaaac tatagaaaga tggaaaaaat cattcgaatt
    20401 ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca
    20461 gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg
    20521 attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta
    20581 aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt
    20641 gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct
    20701 tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc
    20761 gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt
    20821 acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag
    20881 aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc
    20941 ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa
    21001 ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa
    21061 aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa
    21121 gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa
    21181 atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa
    21241 gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt
    21301 ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa
    21361 caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta
    21421 ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct tatcggtaat
    21481 cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt
    21541 cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac
    21601 tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat
    21661 ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat
    21721 agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa
    21781 aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc
    21841 tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc
    21901 ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga
    21961 aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa
    22021 taattccagc catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt
    22081 ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc
    22141 taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc
    22201 gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa
    22261 ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt
    22321 gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa
    22381 taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat
    22441 ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc
    22501 attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac
    22561 tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag
    22621 cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg
    22681 gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct
    22741 gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag
    22801 attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta
    22861 gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc
    22921 ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac
    22981 cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag
    23041 tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa
    23101 aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt
    23161 ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac
    23221 tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc
    23281 actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag
    23341 actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga
    23401 ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc
    23461 aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac
    23521 cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg
    23581 aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt
    23641 cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc
    23701 aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg
    23761 agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact
    23821 gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat
    23881 gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc
    23941 tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta
    24001 caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg
    24061 tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc
    24121 atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac
    24181 gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg
    24241 aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa
    24301 aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt
    24361 tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa
    24421 tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg
    24481 ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat aatataaaag
    24541 aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg
    24601 agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg
    24661 gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga
    24721 ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc
    24781 taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct
    24841 cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa
    24901 gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc
    24961 tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga
    25021 tctggggatt tgattctgat acgacgatta cagttattga agtttatatg agcaacttac
    25081 gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt
    25141 atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc
    25201 ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact
    25261 ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat
    25321 tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta
    25381 aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca
    25441 aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa
    25501 gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg
    25561 aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt
    25621 tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa
    25681 aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg
    25741 attgtttttt ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc
    25801 cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg
    25861 cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat
    25921 actattattg aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact
    25981 ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca
    26041 aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg
    26101 gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt
    26161 gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg
    26221 aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac
    26281 gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt
    26341 tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt
    26401 gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa
    26461 ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa
    26521 aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag
    26581 aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct
    26641 ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt
    26701 caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt
    26761 ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt
    26821 ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt
    26881 gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg
    26941 tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg
    27001 tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc
    27061 gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg
    27121 gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac
    27181 ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg
    27241 ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt
    27301 ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat
    27361 tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt
    27421 cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac
    27481 cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat
    27541 catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg
    27601 tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat
    27661 agatttatga ttggtatatt gttacttata a
//
LOCUS       DAZKAO010000023        27690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000023 DAZKAO010000000
VERSION     DAZKAO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.023"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1916
                     /gene="yfcC"
                     /locus_tag="ACG8H8_000811"
     CDS             177..1916
                     /gene="yfcC"
                     /locus_tag="ACG8H8_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP8985902.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2073..3257
                     /gene="iadA"
                     /locus_tag="ACG8H8_000812"
     CDS             2073..3257
                     /gene="iadA"
                     /locus_tag="ACG8H8_000812"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP8985903.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3368..3571
                     /locus_tag="ACG8H8_000813"
     CDS             3368..3571
                     /locus_tag="ACG8H8_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP8985904.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3672..4334)
                     /locus_tag="ACG8H8_000814"
     CDS             complement(3672..4334)
                     /locus_tag="ACG8H8_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985905.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4347..5849)
                     /locus_tag="ACG8H8_000815"
     CDS             complement(4347..5849)
                     /locus_tag="ACG8H8_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8985906.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6397..8076
                     /locus_tag="ACG8H8_000816"
     CDS             6397..8076
                     /locus_tag="ACG8H8_000816"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP8985907.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8203..8682
                     /locus_tag="ACG8H8_000817"
     CDS             8203..8682
                     /locus_tag="ACG8H8_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP8985908.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8901..9581
                     /locus_tag="ACG8H8_000818"
     CDS             8901..9581
                     /locus_tag="ACG8H8_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP8985909.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9589..10920
                     /locus_tag="ACG8H8_000819"
     CDS             9589..10920
                     /locus_tag="ACG8H8_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP8985910.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10935..11897
                     /locus_tag="ACG8H8_000820"
     CDS             10935..11897
                     /locus_tag="ACG8H8_000820"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP8985911.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12008..12346
                     /locus_tag="ACG8H8_000821"
     CDS             12008..12346
                     /locus_tag="ACG8H8_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP8985912.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12425..13132
                     /locus_tag="ACG8H8_000822"
     CDS             12425..13132
                     /locus_tag="ACG8H8_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP8985913.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13235..14716)
                     /locus_tag="ACG8H8_000823"
     CDS             complement(13235..14716)
                     /locus_tag="ACG8H8_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985914.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15025..15561
                     /locus_tag="ACG8H8_000824"
     CDS             15025..15561
                     /locus_tag="ACG8H8_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8985915.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15639..17009
                     /locus_tag="ACG8H8_000825"
     CDS             15639..17009
                     /locus_tag="ACG8H8_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP8985916.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17076..17411
                     /locus_tag="ACG8H8_000826"
     CDS             17076..17411
                     /locus_tag="ACG8H8_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985917.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17492..18349)
                     /locus_tag="ACG8H8_000827"
     CDS             complement(17492..18349)
                     /locus_tag="ACG8H8_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985918.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18456..18971
                     /gene="tadA"
                     /locus_tag="ACG8H8_000828"
     CDS             18456..18971
                     /gene="tadA"
                     /locus_tag="ACG8H8_000828"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP8985919.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18994..19078
                     /gene="ffs"
                     /locus_tag="ACG8H8_000829"
     ncRNA           18994..19078
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8H8_000829"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19288..20673
                     /locus_tag="ACG8H8_000830"
     CDS             19288..20673
                     /locus_tag="ACG8H8_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP8985920.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20710..21387
                     /locus_tag="ACG8H8_000831"
     CDS             20710..21387
                     /locus_tag="ACG8H8_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP8985921.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21395..22159
                     /locus_tag="ACG8H8_000832"
     CDS             21395..22159
                     /locus_tag="ACG8H8_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP8985922.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22161..22820
                     /locus_tag="ACG8H8_000833"
     CDS             22161..22820
                     /locus_tag="ACG8H8_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP8985923.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22850..23359)
                     /locus_tag="ACG8H8_000834"
     CDS             complement(22850..23359)
                     /locus_tag="ACG8H8_000834"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP8985924.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23471..24469)
                     /locus_tag="ACG8H8_000835"
     CDS             complement(23471..24469)
                     /locus_tag="ACG8H8_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8985925.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24670..25254
                     /locus_tag="ACG8H8_000836"
     CDS             24670..25254
                     /locus_tag="ACG8H8_000836"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8985926.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25331..26506)
                     /locus_tag="ACG8H8_000837"
     CDS             complement(25331..26506)
                     /locus_tag="ACG8H8_000837"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP8985927.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26588..26968
                     /locus_tag="ACG8H8_000838"
     CDS             26588..26968
                     /locus_tag="ACG8H8_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP8985928.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27042..27404
                     /locus_tag="ACG8H8_000839"
     CDS             27042..27404
                     /locus_tag="ACG8H8_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985929.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9000 a         4676 c         5775 g         8239 t
ORIGIN      
        1 ttaatataaa tggaaagaga aaaaaataat catacatagg agaaaaaaaa caaaatagaa
       61 aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt
      121 tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga
      181 agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata
      241 cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag
      301 caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc
      361 gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt
      421 ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg
      481 aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag
      541 atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata
      601 cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg
      661 gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag
      721 ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag
      781 cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt
      841 taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg
      901 taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg
      961 tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc
     1021 cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat
     1081 tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg
     1141 cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc
     1201 gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga
     1261 tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct
     1321 attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg
     1381 ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg
     1441 gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg
     1501 tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg
     1561 ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa
     1621 gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca
     1681 tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc
     1741 ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag
     1801 gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc
     1861 tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact
     1921 ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt
     1981 ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc
     2041 atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt
     2101 ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg
     2161 tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg
     2221 gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag
     2281 aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg
     2341 taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga
     2401 tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt
     2461 accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata
     2521 aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg
     2581 aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg
     2641 ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg
     2701 tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag
     2761 aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta
     2821 gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga
     2881 aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag
     2941 gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca
     3001 aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg
     3061 ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa
     3121 tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata
     3181 cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt
     3241 tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt
     3301 tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg
     3361 aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga
     3421 aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc
     3481 acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga
     3541 caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca
     3601 aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat
     3661 tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc aaagacttca
     3721 tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca
     3781 tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca
     3841 gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact
     3901 cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt
     3961 cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg
     4021 atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct
     4081 tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa
     4141 cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga
     4201 aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa
     4261 ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt
     4321 tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt
     4381 aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct
     4441 aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca
     4501 gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg
     4561 ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt
     4621 tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact
     4681 cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat gattttactt
     4741 ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc
     4801 ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta
     4861 gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat
     4921 tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt
     4981 ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca
     5041 tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg
     5101 aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt gatcgtcatt
     5161 tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg
     5221 tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca
     5281 tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca
     5341 gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt
     5401 cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa
     5461 ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa
     5521 ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga
     5581 gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc
     5641 tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt
     5701 tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa
     5761 ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta
     5821 cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga
     5881 taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat
     5941 gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa
     6001 aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat
     6061 gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag
     6121 tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag
     6181 cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac
     6241 tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt
     6301 gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat
     6361 ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca
     6421 ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt
     6481 tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta
     6541 atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca
     6601 catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca
     6661 gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca
     6721 gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa
     6781 acagtagtga gctttttccg taccactcat acgataccag attcagtggg gatcaatctg
     6841 aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc
     6901 ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc
     6961 ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata
     7021 gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta
     7081 gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa
     7141 gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa
     7201 cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca
     7261 gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa
     7321 atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact
     7381 actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga
     7441 gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct
     7501 aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga
     7561 gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa
     7621 aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt
     7681 gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga
     7741 aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc
     7801 actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc
     7861 gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc
     7921 gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca agacatccga
     7981 gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc
     8041 attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct
     8101 ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat
     8161 ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa
     8221 gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg
     8281 gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt
     8341 aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac
     8401 gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct
     8461 ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga
     8521 ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa
     8581 gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta
     8641 gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct
     8701 aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta
     8761 ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc
     8821 agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga
     8881 gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg
     8941 aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag
     9001 acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc
     9061 acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg
     9121 agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa
     9181 aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg
     9241 aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag
     9301 gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag
     9361 aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag
     9421 aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca
     9481 atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg
     9541 aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa
     9601 catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga
     9661 agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca
     9721 gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa
     9781 ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac
     9841 gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg
     9901 actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg
     9961 gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc
    10021 aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc
    10081 tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc
    10141 aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg
    10201 cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt
    10261 tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta
    10321 gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag
    10381 aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg
    10441 ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa
    10501 gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct
    10561 ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc
    10621 aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt
    10681 agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc
    10741 ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac
    10801 attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt
    10861 attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag
    10921 aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata
    10981 aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat
    11041 tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga
    11101 ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag
    11161 cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt
    11221 tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg
    11281 tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt
    11341 ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg
    11401 gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga
    11461 tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga
    11521 tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg
    11581 caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga
    11641 cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg
    11701 ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg
    11761 aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag
    11821 atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga
    11881 gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat
    11941 gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag
    12001 aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg
    12061 aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt
    12121 catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag
    12181 cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac
    12241 aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg
    12301 atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt
    12361 cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac
    12421 gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct
    12481 tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga
    12541 tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag
    12601 tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa
    12661 ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc
    12721 gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc
    12781 attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc
    12841 cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt
    12901 cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc
    12961 tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat
    13021 ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt
    13081 cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga
    13141 agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat
    13201 ttatttggaa taagaggatg tgacaagaat tttgtcacat cctctttgtt ttgttctcat
    13261 aacctttttt catatacagt tcatgaaccg cttttattaa cttgccaaat aacactgtat
    13321 tgtgcgttgt atccagatag aaagtctccg tttcatctga aattttctca tgctgatctg
    13381 taatgatcaa atcagcatgt tctgaatatg cggtaatttt aacagcatct ttgcgaaata
    13441 actgctgcaa atggctttga agatgataat ttcctgtaaa ctgtttggtt atctgcaaat
    13501 ggatgagtaa ctgctctttc gtttctgttt gtagtaagtg ttggatcaat tgactaaggt
    13561 attgactgat ttggtttctt tccccatttt ttgaaaaaat tgtatcgatt ttttctctta
    13621 atttcttttc ctgtataaaa gaactagaat gattggacgt ggcttctgga aagcaaagtg
    13681 tagaaaaact actgctggat tcacccaaca gttcgaaaaa aacaactgcc aacacaagaa
    13741 aataaaaata aattggtatt ttattttcta aatggttgag aggaaactgt tgagttaagt
    13801 aagtcatcaa ttgaccgact tgtcgacaaa taaggttttt gcttgtggaa aataaaaaac
    13861 caatctcctg tttttgtcga aaagagattt gatttggaaa aaaacaatag aaaaaataat
    13921 taaagtaggc tctctctgtt tctgtctctt cacaagggcg actcactgaa aaagaagtaa
    13981 agtattctga ataatcatgt gtatgctgca ataagtccat caattcagtg atttcagaag
    14041 aagtttttgg aaaatatgcc tgttgtttgt ttcgtaatat gaataaagtt aacagcaagt
    14101 agatattttt ttcttgtttt gaaatttttt ctatttgctc atctgtactg ttcattttta
    14161 gtgcatttaa ttcaagtgag gaaaatggcc aatcgttacc tccaaatgta tctgaaagaa
    14221 aaaagtaaag atagagacgg atagataact catttccttc aagagtacag caatttttta
    14281 cagataggat ccgtaaatgg aattttttta aggaatgatt gagctgtttt gtcagttttt
    14341 cgagatagga gggcgatata taaagttgtt caagtaaata tttttttggt acagcagttt
    14401 gtggataatt gattagtaac atcaaatagt taaaacgatt ggattctttt aggtagcata
    14461 attttaactt agaaaaaaca gctaactttt tagtttgatc ggtttctttg gtttggatct
    14521 ctcctttatg attagaataa ataaattcat caatcaataa aaattgcttg agtgatacat
    14581 ttatttttcg gatcagttct ttgattgtcc gttttggaat atgtaagtca ttggataatt
    14641 cttgtaaaga acaattattt ttttgaatga ccatttgtag aacaagtaac tctttttttc
    14701 ttgcttcaat tagcataaat acctcctttt tattctgttt ttgtatatag ttagacaaaa
    14761 actgtgagtg gaaagtcatt tcagtctgtg ttattctcac agagtgtcgc cttctttttt
    14821 atataacgtt atttaatttt tttgttacct tgtttattct attgtttatt tcctagggta
    14881 ttttgtcaag atattcttgt ttttaaggcg gaaaaattga tttttaatgg atataactaa
    14941 atatgttttg ataattatca ataatatttt tagaattatt tatgttattt atttatgggt
    15001 agatgaaaag agacggaggt aagaatgatg gggagagtaa caaattatag taaagaatat
    15061 ttgctgttca aaagtatggt atacgtggaa gaatatggaa tgaagtcaat tactgcaaga
    15121 gagttagcag agttctgcgg ttgttctaca tacccgattt atacacattt caaatcaatc
    15181 agcgggttaa aagagaagat attagaagaa atcactgttt attttgaaaa ctacttagct
    15241 gaatgcagtt ccgatgatgt tttaagcatt gtctacttat tgaaagattt tttttctatc
    15301 catgaaagta ttcgtgaaat cattgcaaaa tctgatttag acatggattc gctatttaag
    15361 cggccatttt tctcatatgt caaaggtcac acgaagatca aagaagaaaa gtcgcttgaa
    15421 ttggtatggc ttaatgcatt gggaagtttg cgctcagatg attcttctca agattttctt
    15481 gaaatgatga acgctgtttg ggataaaaac gaggtgacgg atgaatactt ggaaaatatc
    15541 ttaaaggaac agtcacctta ggcagcaaca tgaaaaagta gatcagccac ctttattttt
    15601 cgtaaaaaag attgtaaaat gtctaacgaa acattggaat gaaggaggaa gcgacaacgt
    15661 taccacagca atttatcaaa aaatatcgtc ttcttttagg cgaagaagca tccgattttt
    15721 tttctgcgct tgaacaagga agcgtgaaaa aaggatttcg gtggaatcca ctaaaaccgg
    15781 caggattgga catggtccaa acttaccata gcgaagaatt acaacccgct ccctacagca
    15841 atgagggatt tcttggcact gtcaatggaa agtctttcct ccatcaagct gggtatgaat
    15901 atagccaaga accaagtgca atgatcgttg gaacagctgc tgcagcaaaa cccggtgaaa
    15961 aagttttgga tttgtgtgcg gctcccggtg gtaaatcaac ccaattagct gctcaaatga
    16021 aggggaaagg gttattagtg acgaatgaga tctttccgaa acgtgcgaag attttatcag
    16081 aaaatataga acgatgggga gtttcgaacg caatagtgac aaatcatgca cccgcagaac
    16141 ttgtccctca tttttcgggc ttttttgatc ggatcgtggt tgatgctcct tgttcaggag
    16201 aaggaatgtt ccgaaaagat ccaaatgcaa taaaagagtg gacagaagaa tctccgctat
    16261 actgtcaaaa acgtcagcaa gagattttgt cttcagcaat aaaaatgcta aaaaataaag
    16321 gacaactgat ttattcgact tgtacgtttg caccagaaga aaatgaagag atcattagct
    16381 ggcttgtaga aaattatcca gtaacgattg aggagattcc tttgactcaa tccgttagtt
    16441 caggacgttc tgaatgggga agcgtagctg gtttggaaaa aacaatacgt atatggcctc
    16501 ataaagatca gggtgaagga cattttgttg caaagctgac ttttcatgga caaaatcaga
    16561 tgcataaaga aaagaagaca cgaaaaaaaa gtaaagtgca aatgacaaaa gagcaggaaa
    16621 aactctggac ggagttttca aatgattttc attatgaagc tactggaaga cttttagtgt
    16681 tcaacgacca tctttgggaa gttcctgagt tagccccttc tctcgatggt ttaaaagtcg
    16741 tacgtacagg tttgcattta ggagatttca aaaagaatcg atttgaacct agctatgcat
    16801 tagcgcttgc tacaaaaaag atagagaata taccttgctt gccgatcact caaaaggaat
    16861 ggcaatctta cacagcagga gaaacatttc aacgtgatgg gaatcaagga tgggttttgc
    16921 tagtattaga taagatacct gtcggatttg ggaaacaagt aaaaggaaca gtaaagaact
    16981 ttttccctaa aggattgaga tttcactgag tggattagtt cgtttatttt tttgtctatg
    17041 ctatgatgga gatagataaa aaggagctga tacttatgaa aacatcaaca aaagtaacaa
    17101 tcggattaag cgtagcagcc gctgccagtg tagcgacagc agtcgttgta tcgggaaaag
    17161 taatggaaaa gatccatcat atgactactc ggacgaaagt aaaaaaattc gtctatgaca
    17221 aatttgatgg aaatgaaaaa ttactagaaa tcgtagatgg tctttcagat agtgacttgg
    17281 attctttgat gggaatgtta gcaaaaatca aatcaggaaa aaagaaaatc tctgtttatg
    17341 gtgattcaat caaagattca accgaagatg taaaagaccg gctaatgcat tttgttgaaa
    17401 aaatgatgta agagaaacta gcttttgaac actattcatt cgaaataagg ggttgtgaca
    17461 aaattattgt cacaacccct ttaatctatc tttaattatg ttcatgaagc aataaaagat
    17521 agcaaaacag taaatcgttg gcttttttta gattgaaacc agactgttct tgaaattttt
    17581 cgattcgata ttttagagta ttacgatgaa gaaacagtgt ttttgcagca gagctgacat
    17641 tgcctaattc attccataat tcttgaatga tcacttccat ttcattttgt ttgatcagtt
    17701 ctatgtaact tttgatcaac ttactatctt ttacagtatc ttttgtatag taatgtaaag
    17761 ccacatcttg catagaaaaa gtacggctgt gagtataaag attgttttgc tctgttaaaa
    17821 aaatttttct ttcttcagca aacaaaggga cgaggtcggt gccaggtgaa tggaaagaac
    17881 caacgaatac agacgttgtc gtatcaaaat cagcatctaa agatagaaaa ataccgttga
    17941 tttcagtagc atcaagataa tcacggttta tttgttcgac taagagcgca tctgtgtctg
    18001 tataaaaaaa gaaatcagca gaatagcgaa ataattccaa aatagtatct tgccattctt
    18061 tttttaagaa ttctcctttt gattcgacgt gaagctggat caaacgaaag cttttttctt
    18121 gaacacaaga agcatcttta aaaagatagt gataccatgg gtgttttttt tgatcgttga
    18181 ttacagggaa taacgctttc aaaaggctgg tttcttgttg agataatgaa tcttgtttga
    18241 tccagataaa agcatcatca attgcaaaag aaaggatatc cgcttctgat gctggagagt
    18301 tttttttctg tgcttgtggg taaagagcaa gtaatttatc cattttcatc ttgttcacct
    18361 cttgtattta ttttaccata gaaaaaaaag ttagttacag cctttttatc atatgctaaa
    18421 gtaaaacaag aaaaaacaaa ggcagggaag ggtaattggt aaatacaaat cttgatgcag
    18481 tagaaaaaga aaaatggatg cgccttgcat tggcagaagc aaagaaagca gaaatgcttc
    18541 atgaagttcc gattggagca gtggttgtat tggatggaaa agtgattgga agaggatata
    18601 accttagaga aaccacgcaa gatgctacta cacatgcaga aatgcttgca atcaaggaag
    18661 cctgtgaaaa agtgggaagc tggcgcttgg aagatgctgc actttttgtt accttagagc
    18721 cttgtcctat gtgcagcggc gcgatgattt tgtctcgtgt agcagaagtt tatttcggcg
    18781 cgtatgatcc aaaaggcgga acagccggta ccttgatgaa tcttttggag gacgagcggt
    18841 ttaaccacca agcatatgta gaaggtggca ttttagaaga cgaatgtggg atgattttga
    18901 ctgacttttt ccgaaagctc cgccagcgaa aaaagaaatt aaaaaaagac tggtcaacga
    18961 atgaaaaata aggtatacta ttctttgccg aaaggctagg acaatggtgg gcttgtgaag
    19021 cgtgtcaggt ctggaaggaa gcagcactaa gcaggttccg ccatgtgtct gattataatg
    19081 aaacgacaac agactttcgg gtctgttttt tttattttta tagtactgta aaaatgaatg
    19141 ttcctgattt tttagccaga ctgatttatc gaatattgta cttatacaat ttgcttcaag
    19201 gcaggaaaaa ctctgaaata gagcttgttt caatggaaga aaaagctgcc tatggtatga
    19261 taaagataaa gagaggcgag agggtacatg gaaaaaatag tgattttaca tacgaatgat
    19321 ctgcattctc atttagaaaa ttggccaagg atcagaaggt ttttggatca acgtaaacga
    19381 gaaaacgaaa agaaagaaaa aacgacaacg attgcagtag atctaggtga ttttgtggat
    19441 cgttggcatc cattgtcaga agcaacgaat ggacaggaaa atatcttgct gatgaatgaa
    19501 gttggttatg atgcagcgac gataggaaac aatgaaggcg taggaaattc caaagaagag
    19561 ttgaatcatt tatacgatca tgctgagttt gatatcatcc tggataactt attcgataaa
    19621 aatacgctcc agccaccggc atggacgaaa ccatataaaa tcattacaac gaaacaccag
    19681 acgaaaatcg gactaattgc atttactgct ccatttccgc ttacttataa tccgaatggc
    19741 tgggacatac ggaatcctta tgatattttg ccagaattag tcgaagattt acgttcacaa
    19801 gtggatgttt tagttttgat gagtcatctc gggattcagg atgatttcca aattgcaaaa
    19861 gaaataccag caattgatgt tattttagga tcgcacaccc atcatttatt cagagaaggt
    19921 cacgtaatca atgatgtcca aatagcagcc gctggaaagt acgggcagta tgtcggtgaa
    19981 gtccatttaa ccatcgatga agagaaaaaa atccttaagc attcagccaa agccatccct
    20041 accgaaacaa tgacagcttt tacagaagat gaacaagaaa taaatagtta tctagaacgt
    20101 gggcatgaat tattaaaaga aaaagaagta gcagatttgc cattttctct gagtctagat
    20161 atcttcaatg agcattcgtt tatccaagta gcctttgagg cggtgaaaga acgaggaaag
    20221 acggaagcag caatattaaa cagtggtctg tttttaactg aaattccaaa agggcgggtc
    20281 aatcaggatc agcttcacac tgcgttgccg catccgatgc acttgttaaa tgtcacattg
    20341 ggtggaaatg atctgatccg cttagtttta gaaatagaaa aaaatcgtaa ctttttacgt
    20401 aactatccta tgaagggaat gggatttcgc ggtaagattt ttgggcagat ggtctataat
    20461 ggaatcacat atgattctat taaccatcaa gtcttttgga agaacaagcc aatcgatctg
    20521 aataaaaatt atacttttac taccgtagat catttgatgt ttgttccatt ttttccaacg
    20581 attgagattg ctggaaaaaa tgaatttttg ttccctgaat ttatccgaag tgtggtagga
    20641 gattacttga aagctcacta cccaatcaaa taaaaacaag gtataataaa aaagataggt
    20701 ggtgaacgca tgtctgcaaa acaaaatgaa cagcagaacg ttgagcataa aacagaaatt
    20761 gctaagaaaa aatcatcagc tgaacaggaa acaacagtca ccgaagaact gacagctgct
    20821 ttggaagaaa tcgttgagga gcctaaagca gaaaaagtca aaggcgagct agtgacactg
    20881 ttagatgaca gtaatttttt gataggtgaa agacattatc ggcttgtgtc cgattatcgt
    20941 gaaggattta atgcagaaaa gcttggagaa cgttacagtg atgtactagc ccgatatgat
    21001 tatatcgtag gagattgggg ttatgaacaa ctacgcctaa aaggattttt ccaagcggat
    21061 aatcgacggg cgcatccaga tcagcgaatc gattccttgg aagactatct ttacgaatat
    21121 tgcaattttg gttgtgcgta ttttgtcatc gagagaatcg gcggtaaaaa agaaaagaca
    21181 actcagcgcc gaaaaaagaa aaaaaaccat aatcgtgcgt atatcgatga gaaaaaagga
    21241 ccagtcgcat ctaatcgcaa aaaaccggtt attaaaaatc gaaaaacaga gtcagcaaaa
    21301 acctctgaaa aagtaaaaaa acaagaacaa cctaaacaag tagaaaaaac aaaaacagca
    21361 tttacgatca gaaaaaggga agagtagtgg atgaatggac tataaaggat atttaattga
    21421 tttagacgga acgatttatc gtgggacaca acccataccg gccggtaaac gatttgtgga
    21481 agaattgcaa aaaagaaaat tacctttctt gtttgttacc aataacacga ctaagtcacc
    21541 agaaacagta gctaatcgtc tagcagatga gtttgacatt catgtagctc cagagacagt
    21601 ctacacagct actttggcaa caatcgattt tatgaaagca gatggaaaag ggaaaaaagt
    21661 ctatgttatc ggagaagcag gattgattga cttgatttta gctgccggat ttacatggga
    21721 agaagaggct cctgactatg tagtggtagg cttagataat tatctgacct atgaaaaagt
    21781 agttaaagca acacttgcta ttcaaaaagg tgctactttc attggaacga acccagataa
    21841 gaatatacct actgaacgcg gccttcttcc tggagcaggg tcagtcatct cttttgtaga
    21901 gacagcaacc caaacgccgc cgatctacat cggaaaacca gaagccatca ttatggataa
    21961 agccgttgaa gtattagggc tgcaaaaaga agaagtaatc atggttggag ataactatga
    22021 aacagatatc caagctggga tacgcaataa tatagatact ttgctcgttt tatcaggttt
    22081 tacaaaagaa gaagatgtac ctgggttgcc tatcccagct acctatgtga aacaatcact
    22141 ggatgaatgg agcttctaat atgaaaaatt cgagatggca gtggatggaa tatgctggat
    22201 tgttcagcct atttctgaca ctgatcagtc tggcagttgg tgtgacgatc aactttcgac
    22261 cactatacgt atttgatatc ggacatttgc aaattttgga ttatacttct ttagatcaag
    22321 agacgttact gaaaaatttt gatcacctaa tgaattatct caataatccc ttcaaaacca
    22381 tcctcagctt gccagatttc ccagtttctg ctagcggagc ccatcacttt tatgaagtga
    22441 aaattttgtt tttagtggat tatgctgtgt ttttcatcac tttgatcccg agtattttgt
    22501 ttatcaaata tttgcaaaag aatgatcggc tttggcggtt gattcgtccg tttcaaatcg
    22561 gcatgttact gccagttgtt ttcggttttt tcatgatgat aggatttgat cgctttttta
    22621 tcctttttca tgaaacgttt ttcaacaacg atgactggtt attcgatccc gttactgatc
    22681 caatcatcaa tgtacttcca gaagaatttt tcatgcatag ctttatctta ttttttgtat
    22741 tattagaatt attttttgct gtttttcttt tcttaggaaa aaatagcttg aaacaaacaa
    22801 aaaagaaaga actcgtctaa atagacgttt tctttctttt tttgttgaat tattgagtaa
    22861 ggcgacttct tttttctggt tctttgtgtg ctaatcgact ggcagcagca gcggcgatcg
    22921 ctccgacgat atcatctaag aaggtatgga catgaacgcc atcgtggctg tttagttctt
    22981 ttaaaatacc aggtttcacc ttgtcgatat aaccataatt cgtaaagccg atcgtaccgt
    23041 aaacattcac gattgacaag gctaggatct catcgatacc gtaaaggcct tcgtcatctt
    23101 cgataatatg ttgcaacgga ctcatcaatt ttttcttttc tgctaatata tctaattgga
    23161 ttccagtgat aatggcgtta tgtacttcac gctttgttag cacgctgttg acactttcag
    23221 tacaatccgc caaagtcaaa tcagaaatat agtctttttg taaaaacatg acaagttgtg
    23281 cgatatcttc tacagttacg ccacgttcct tcaatagttc tcttgccttt tcttctaaag
    23341 tctcagtttt gacgaccata ttattgctcc ccctttattt tttctttctc tatttcatgt
    23401 taacggaaca tgaaaaaaat gcaagaaact gctttcgggt ttaacttgaa atgcaaagag
    23461 atataaaaaa tcaactagga cgaagatttt tatcgtataa gcttgtgctg tgaccaggct
    23521 gagttcgttc ctttggatta atatagtgta aagcgttgtt cactgcagta ggtgcttcac
    23581 cgagcccagt agcaatcagt ttcacttttc cttcataggt acagatgtca ccagctgcat
    23641 aaacgcctgg aatagaagta gacatatctg attttacagt aatggcattt ctggtactat
    23701 caagtcccca agaagaaaga tgttctaaat tagaagagaa tccataattg acgatcagag
    23761 aatcaatcat caaatcaatc gtctcgtcac ttttcacttt ttttaaccgg atatctgtca
    23821 gttccccatt attgcctgat aatccatcga tgagataggg agtaagcaag ttgacagatg
    23881 aagatttcaa gcgtgataca ctatgttcgt gcgcacgaaa ttccgggcga cgatgaatca
    23941 agtaaacttc gcttgcaata ggctctaaca tcaacgtcca atcgatagca gaatcaccac
    24001 ctccagcaat agcgactttt tttccagcat acttcatcaa atcattcacg aaataatcca
    24061 atccgtggtt ttcaaatgat tcagcattat ctaaattcaa ttttcttggc tggaatgaac
    24121 catttcctaa agctaaaatg actgctttgg aataatggat gcctttattt gtagtaatct
    24181 cgataacttc gtcttcacga gtaagcgaga gaacttcttc ttttaaatgg aatgtatggt
    24241 taaaagtagt taattgtttt tctaattgat cgattaattc acttgctttt attgctggat
    24301 acccaggaat atcgtaaata tatttttcgg gatacaaagt tgctaattga ccaccaagtt
    24361 gaggtagact gtcaatgatt tttgttttag cttggcgcat tcctgcgtaa aaagcggcaa
    24421 acatccctac aggacctgca ccgatgattg taatgtcata gatttccata aaaattcctc
    24481 ccttttcaca ctatagcaga atttaattga gaatgcttct caaaaatgtt tgaattcgaa
    24541 atcgaaacgg taagttatag aatgtatcta gtgatgaaaa ataaaagaaa acaaatctag
    24601 tatttttacg agaggttagg tattatgtta ttattattat gaagtatgca aatattagga
    24661 ggaaacaata tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt
    24721 atcaaaacaa accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg
    24781 gttcaaaatt tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc
    24841 gttattcctg atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa
    24901 agtatttatg gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga
    24961 gctttatcta tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat
    25021 aacacaaatg tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa
    25081 atcattgaag cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt
    25141 ggacatgtac tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca
    25201 caagaccgac ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt
    25261 atggtacgga gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg
    25321 aataaatgat ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta
    25381 aagtttcttc tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg
    25441 tgatgccagg atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt
    25501 cgtctgaaat ataggcggta aaatctaacc acattaagta agttccttca gggttcatta
    25561 ttctcacttt cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta
    25621 gataaggaat cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa
    25681 tcagtccaaa agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag
    25741 ctttcatctc tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag
    25801 tcttggttgc ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca
    25861 tggaagtaaa tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa
    25921 tcacttgatg ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac
    25981 gccctccagg attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt
    26041 ttttctccat gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc
    26101 gaacgatgtg acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat
    26161 gaatcaaaat agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta
    26221 acacaccact tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt
    26281 cccaatgaat gatagcctga tatagtttat cagacagagt ggcataacca aagattccat
    26341 gtttgatgta ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg
    26401 ctacccacaa aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta
    26461 cactttctgt atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag
    26521 tcattttcgt cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg
    26581 gaagtagatg acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc
    26641 ttatggcgca ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt
    26701 acaatccggt tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt
    26761 tcagatcatc gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga
    26821 aaaaacaaat ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa
    26881 gatcggattt accactattg cggagcagtt acctatttgg atagaagaag cattatctga
    26941 actagaacgg aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat
    27001 ttttatgtaa gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg
    27061 ctggtacaat tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg
    27121 atgacaagtt ggaatttgcg ttagcggaac tttctgaagg aaagactggt ttagcgaata
    27181 tacttaattt cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat
    27241 tgacaaatac acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa
    27301 agaatcaaag agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct
    27361 ctgttcttgg aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga
    27421 acaaagaagt ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa
    27481 tgaaatgttc tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac
    27541 ggttgtataa cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt
    27601 tatagttgac caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg
    27661 ttatatcaca tttctaaaaa atttaaaaaa
//
LOCUS       DAZKAO010000024        27232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000024 DAZKAO010000000
VERSION     DAZKAO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.024"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..445)
                     /locus_tag="ACG8H8_000840"
     CDS             complement(161..445)
                     /locus_tag="ACG8H8_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985930.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="ACG8H8_000841"
     CDS             complement(463..1353)
                     /locus_tag="ACG8H8_000841"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP8985931.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="ACG8H8_000842"
     CDS             complement(1373..1591)
                     /locus_tag="ACG8H8_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP8985932.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="ACG8H8_000843"
     CDS             complement(1561..3261)
                     /locus_tag="ACG8H8_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP8985933.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="ACG8H8_000844"
     CDS             3528..4301
                     /locus_tag="ACG8H8_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985934.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACG8H8_000845"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACG8H8_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP8985935.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="ACG8H8_000846"
     CDS             complement(5125..6096)
                     /locus_tag="ACG8H8_000846"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP8985936.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACG8H8_000847"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACG8H8_000847"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP8985937.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACG8H8_000848"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACG8H8_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP8985938.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="ACG8H8_000849"
     CDS             complement(9051..9875)
                     /locus_tag="ACG8H8_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP8985939.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="ACG8H8_000850"
     CDS             complement(9848..10528)
                     /locus_tag="ACG8H8_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8985940.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="ACG8H8_000851"
     CDS             complement(10551..11489)
                     /locus_tag="ACG8H8_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP8985941.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACG8H8_000852"
     CDS             complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACG8H8_000852"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP8985942.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACG8H8_000853"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACG8H8_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP8985943.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="ACG8H8_000854"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="ACG8H8_000854"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP8985944.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACG8H8_000855"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACG8H8_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP8985945.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="ACG8H8_000856"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="ACG8H8_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP8985946.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="ACG8H8_000857"
     CDS             complement(19463..20704)
                     /locus_tag="ACG8H8_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP8985947.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="ACG8H8_000858"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="ACG8H8_000858"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP8985948.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="ACG8H8_000859"
     CDS             complement(21731..22654)
                     /locus_tag="ACG8H8_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP8985949.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="ACG8H8_000860"
     CDS             complement(23392..24678)
                     /locus_tag="ACG8H8_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP8985950.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="ACG8H8_000861"
     CDS             complement(24671..25939)
                     /locus_tag="ACG8H8_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP8985951.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8H8_000862"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8H8_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP8985952.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="ACG8H8_000863"
     CDS             complement(26711..27157)
                     /locus_tag="ACG8H8_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985953.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7741 a         5873 c         4723 g         8895 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tc
//
LOCUS       DAZKAO010000025        27046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000025 DAZKAO010000000
VERSION     DAZKAO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.025"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..691)
                     /locus_tag="ACG8H8_000864"
     CDS             complement(44..691)
                     /locus_tag="ACG8H8_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985954.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(870..1589)
                     /locus_tag="ACG8H8_000865"
     CDS             complement(870..1589)
                     /locus_tag="ACG8H8_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8985955.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1720..2871)
                     /locus_tag="ACG8H8_000866"
     CDS             complement(1720..2871)
                     /locus_tag="ACG8H8_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP8985956.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3088..3828)
                     /locus_tag="ACG8H8_000867"
     CDS             complement(3088..3828)
                     /locus_tag="ACG8H8_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8985957.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3832..4155)
                     /gene="rsfS"
                     /locus_tag="ACG8H8_000868"
     CDS             complement(3832..4155)
                     /gene="rsfS"
                     /locus_tag="ACG8H8_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP8985958.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4212..4805)
                     /gene="yqeK"
                     /locus_tag="ACG8H8_000869"
     CDS             complement(4212..4805)
                     /gene="yqeK"
                     /locus_tag="ACG8H8_000869"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP8985959.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4789..5439)
                     /locus_tag="ACG8H8_000870"
     CDS             complement(4789..5439)
                     /locus_tag="ACG8H8_000870"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP8985960.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5449..5760)
                     /gene="yhbY"
                     /locus_tag="ACG8H8_000871"
     CDS             complement(5449..5760)
                     /gene="yhbY"
                     /locus_tag="ACG8H8_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP8985961.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5776..6885)
                     /gene="yqeH"
                     /locus_tag="ACG8H8_000872"
     CDS             complement(5776..6885)
                     /gene="yqeH"
                     /locus_tag="ACG8H8_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP8985962.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6882..7409)
                     /locus_tag="ACG8H8_000873"
     CDS             complement(6882..7409)
                     /locus_tag="ACG8H8_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP8985963.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7589..8374)
                     /locus_tag="ACG8H8_000874"
     CDS             complement(7589..8374)
                     /locus_tag="ACG8H8_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP8985964.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8367..9236)
                     /gene="accD"
                     /locus_tag="ACG8H8_000875"
     CDS             complement(8367..9236)
                     /gene="accD"
                     /locus_tag="ACG8H8_000875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP8985965.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9237..10616)
                     /locus_tag="ACG8H8_000876"
     CDS             complement(9237..10616)
                     /locus_tag="ACG8H8_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP8985966.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10624..11043)
                     /gene="fabZ"
                     /locus_tag="ACG8H8_000877"
     CDS             complement(10624..11043)
                     /gene="fabZ"
                     /locus_tag="ACG8H8_000877"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP8985967.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11068..11544)
                     /gene="accB"
                     /locus_tag="ACG8H8_000878"
     CDS             complement(11068..11544)
                     /gene="accB"
                     /locus_tag="ACG8H8_000878"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP8985968.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11547..12782)
                     /gene="fabF"
                     /locus_tag="ACG8H8_000879"
     CDS             complement(11547..12782)
                     /gene="fabF"
                     /locus_tag="ACG8H8_000879"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP8985969.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12801..13538)
                     /gene="fabG"
                     /locus_tag="ACG8H8_000880"
     CDS             complement(12801..13538)
                     /gene="fabG"
                     /locus_tag="ACG8H8_000880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP8985970.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13550..14467)
                     /gene="fabD"
                     /locus_tag="ACG8H8_000881"
     CDS             complement(13550..14467)
                     /gene="fabD"
                     /locus_tag="ACG8H8_000881"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP8985971.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14574..14798)
                     /locus_tag="ACG8H8_000882"
     CDS             complement(14574..14798)
                     /locus_tag="ACG8H8_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP8985972.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14851..15813)
                     /locus_tag="ACG8H8_000883"
     CDS             complement(14851..15813)
                     /locus_tag="ACG8H8_000883"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP8985973.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15823..16263)
                     /locus_tag="ACG8H8_000884"
     CDS             complement(15823..16263)
                     /locus_tag="ACG8H8_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8985974.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16569..17534
                     /locus_tag="ACG8H8_000885"
     CDS             16569..17534
                     /locus_tag="ACG8H8_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP8985975.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17591..17821)
                     /locus_tag="ACG8H8_000886"
     CDS             complement(17591..17821)
                     /locus_tag="ACG8H8_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP8985976.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17907..18794)
                     /locus_tag="ACG8H8_000887"
     CDS             complement(17907..18794)
                     /locus_tag="ACG8H8_000887"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8985977.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18883..20106)
                     /locus_tag="ACG8H8_000888"
     CDS             complement(18883..20106)
                     /locus_tag="ACG8H8_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8985978.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20123..21169)
                     /locus_tag="ACG8H8_000889"
     CDS             complement(20123..21169)
                     /locus_tag="ACG8H8_000889"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8985979.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(21333..23060)
                     /gene="ptsP"
                     /locus_tag="ACG8H8_000890"
     CDS             complement(21333..23060)
                     /gene="ptsP"
                     /locus_tag="ACG8H8_000890"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP8985980.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(23060..23326)
                     /locus_tag="ACG8H8_000891"
     CDS             complement(23060..23326)
                     /locus_tag="ACG8H8_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP8985981.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23805..26039
                     /locus_tag="ACG8H8_000892"
     CDS             23805..26039
                     /locus_tag="ACG8H8_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8985982.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26187..26507
                     /locus_tag="ACG8H8_000893"
     CDS             26187..26507
                     /locus_tag="ACG8H8_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP8985983.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26712..26936)
                     /locus_tag="ACG8H8_000894"
     CDS             complement(26712..26936)
                     /locus_tag="ACG8H8_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP8985984.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         7529 a         5873 c         4647 g         8997 t
ORIGIN      
        1 caataataat tgcagaaaaa gtaattcaat catttaaggt catttattct atatataaat
       61 ccaaatctgt ttcaggatat tttaatttga caagcgaaat aatcattttc agttcttcca
      121 tgttttctgg atattctggg tcaaatacct tgctatgacg gccaatcccg ccattgtcga
      181 tcaatcggta aattcgattc cattcaatga attggtcaat tgattggaaa ttgctgattg
      241 gatagccgcc acaatccaaa atctctgata cacgaaatgc taggtatgca ctatcgatca
      301 ctttattttt tttcgtgtct atcttctcat ttctttcgat aagaattttt tcaggacgat
      361 gtttcaagct ccactctaat agtagaaaga aagcctccat ccgatactct tcttcaaata
      421 accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat cagataattt tgtctataaa
      481 tcagcattgg tgcgcggtcc accatacact ctaaataacg gttcagatac cctgtaccga
      541 catagtttcc taatcgatag ccttcaacgc cgaatcttgc gtatttttgc gctaattttg
      601 aacaatcagt tacgttcaca gctaactctt ctactttgcg gtaaccgctt aagatacgtt
      661 caaaaggttc taaagtgatt tttgcttcca tattcttccc cgtccatctt ccacatactt
      721 catatcattt cacatgatac ttatgctctc atcataccac aggttgaatt agaaaagaaa
      781 ataaatcaca caaaaaagca agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag
      841 actgaatact tttcgttaaa actggttgct tagtcttcta atgccacatt atggaacaca
      901 tgctgtacat cttctagatc ttccagtgca tcaatcattt tttcaaatgt ttcttgatct
      961 tctggagata gtgttacctc gttttgaggg atcatttgga tctcagcgat tgaaaaatca
     1021 gtgattcctt tttcttttaa tgcttcttgt atactatgga aatccgctac ttcaccgtaa
     1081 acgatgattt gtccttcttc ttccattaca tcgcgaacat cgatgtcttt ttccattagg
     1141 tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa agattgctgt attgtcaaac
     1201 atgtaagaaa ccgctccgga aacacccatg ttcccgccat ttttcccaaa tgctgcccgt
     1261 acgtctgctg ctgttcgatt cacattgttc gtcaatgtat caacaatcac cattgagccg
     1321 cttggtccaa atccttcata gcgcaattca gagtatgttt catctcctgc accttttgct
     1381 ttttcgattg ctcggtcaat aatatgtttt ggtacattat aagttttagc gcgatcaatg
     1441 acgaaacgta gtttttggtt tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag
     1501 atttcaatcc caaattttgc ataaatccgg ctattattag catctttagc agttttcttt
     1561 gctacgatat ttgcccattt acgtcccata ttttcacttc gctttctgat ttgatagttt
     1621 cataacgcaa aaacgtcctg ttccattata cttgtgaatc acgattctgt gaagcttttt
     1681 ttctttcttc cttcattctt ctcattttta gtcaattatc tatactcgga ttgggatttg
     1741 cccgaaattc tgttccggaa tattgcctcc caattgatag atttgatcac ttctaactaa
     1801 cagttccgct tcttgtgcct ctttttttcc gattcgggaa taaatagaaa tctctttttt
     1861 ttcttttaag aatcttcttc cagcatcggt aaatccaagt agtcgaagat aatttttatt
     1921 ttgttggaca ggaatctctt gctttttgat attcagcaag acataacaag ataatcgttg
     1981 gatccgtgtc catgtatatc tttttgtttt tacctttgac acaaaatcct caaaactatc
     2041 agcagttgca gctgctgttt tcatgcgcga ttctattcct tcggtcatct gatagatatc
     2101 tgtcagttcc agcaaagtag aagataacaa ccgataacgc aaatacggcc aataagattc
     2161 ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc tgaggcggca caagtccagc
     2221 tattcttttt ccttcttcta acgcatggcg aatggctgtt gcactggcta tcctctgatc
     2281 tgtgatgatt ttttcatgat agcctgcact tttacgtcgg atcggatgta attccatagg
     2341 gtggacatac ttggcatttt cctttgcata acttaatccc aagatatgat ttggcgaaga
     2401 aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc tcagtcattt tttgcggata
     2461 actatacgtt tgatcagaca gttcttgaaa tgcctcttct atcctatctt gattctcttg
     2521 gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa
     2581 actgtcacag tccagtgttt gcaagatttt tactgcacct ttagcaaaat agtcagctga
     2641 ctgaaccgcc cattcgacgg gaagctccac tactaaatcc acaccatttt gtaaagcttc
     2701 attcgcgcga tgccatttat cgatgatagc cggttcgcct ctttgcaaaa aattcccgct
     2761 cattacagca atgacgacct ctgctcctgt tatttttcgt gccatttcca catgatagcg
     2821 atgtccattg tgaaaaggat tgtactctac gataatcccg cagcttttca tgcgcatcct
     2881 ccttcatagt tatcgataaa aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa
     2941 aaatacccga actatataaa ctattctcgc aaacacaata cttctgcgac gaaaagctgc
     3001 actgcaagag caatgcctat atcgttcgga tatataattt atgttgaaga tttattcttt
     3061 tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat gctacaaaga accagcgctt
     3121 tgatttctct gatgctgctt cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc
     3181 gctgttttcc agcattctta aataactttc caatggatag gtacgctctt gatgtagttc
     3241 atcttcacga ataaatcgac cagacccttc ttgttcttca acgaaaaagg taagaaaatg
     3301 ttcgatgctg tgtttctcgc ttcctggata actatcccac aaaaaagcaa aattatcccc
     3361 ttgataatga tagctatatt ctggaaaaac ctcatctact tgataggtgg agtgtacgtc
     3421 aaataaaaat cggccatttt cttctaacgc ttgatagact tcatcaaaca cttgctgtac
     3481 ctcttgtcta tttttcatat agcacagcga atcagaaaaa caggtaattg cctgatattg
     3541 tccgatttct gataaatcga gcatattgcc ttccacaaat tggatcgatg cgtcttcttc
     3601 atatgctcgc tggctggcaa tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt
     3661 atatccatct tttgcaaagg caaccgctaa tgctccagta ccgcatgcca gttccaaaat
     3721 attttttgtg tcttcttgta aatgacgttt cgaaaaagct aaccattgct gataaagtgt
     3781 ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca taagccatat gttaatccaa
     3841 ccattcacta agatcgacta gcggagcatc tgaccatagt ttttccagat tatagaaatt
     3901 tcgttctggc gcatggaaaa catgtacgat cacatctcca agatcgatca atacccattt
     3961 tccgccttct tttccttcaa tacgttttac ttcatatttg ttttcttctt ctttatcaat
     4021 gatttcttct gtaattgcgt tgatttgacg ttcgctattt gctgaacaga tcatgaaata
     4081 atctgccaac aaggaaacat ttcgaacgtc taaagcgata atgtcttctg cccgttttga
     4141 atctgctgct tcaatcgcaa tttttaattg ttctttacta tctatgatga tttcctccta
     4201 atatgagcag cttatttttt tgctacccag taattatatg tttcgattgt ttttggataa
     4261 atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct
     4321 tcgtctagat cagcccatgc aatggctcgt gcatcttgta ccccaggaaa atctcgacct
     4381 ggttcaatat agtctgctac ataaatgatt ttgtcaagga gactcatctt agcagcccca
     4441 gtcgtatgca cccggattgc atgaagaatc tctgcatcgg tgattcctaa ttcccgctct
     4501 acaaacgaag cgccaacaag accatgccag attgcattat tataattcaa taactccgga
     4561 tcaaaaccgt ctcttacgat taccattttg aactcctcat ctggtcgttc ttttgcataa
     4621 tcatgtgtca atgcagcaat actagctttt tcaggagaag caccgtattt tttggcaaga
     4681 gccacggctg tttcttctac tccaagcaca tgcttgaagc gttgttcgct cattctcatc
     4741 tgtatttttt gcatcaggtt ttctctagaa taagcagtat attcattact atagtccata
     4801 ttcatacaac ccttcatttt gaatataatc aattacttta tctggaacaa ggtagcggat
     4861 agaacagcct tctttgatct tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac
     4921 atctacccaa attacaggat atggagtatc cgttgtgtat cctggtcgac gtatacccac
     4981 aaagttgacc atagaagtca gctcatcgat tttataccat tttggcaaat attctaccat
     5041 atccccaccg ataataaagt aataatctgt atccggattg ttttgcgtca attccttcat
     5101 agtatcatag gtgtaacttt tcccaccacg agctaattca atcgtttcaa tctgcaaaaa
     5161 aggattgtct tccaccgcta attccaacat gttgagtcga tgttttgcat caatcgtctg
     5221 tttctcatca acatgaggtg gctgataaga aggcatcaaa aatacgcggt caagacctaa
     5281 attccggcca gcttgttcag ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc
     5341 gcccaagatc cctacttgct ttttcttctc taagaattga ggcatttctt caggaaataa
     5401 ttgtgaacga acaaatgtct tagcttgcgt attcatcatg gtcactcctt atattgcttt
     5461 tacttctttt gatagccttt gatacttttc ttttgtggaa ggtttgtata atacaataac
     5521 acgtccaatg atttggacga tttcgcaatc tatttctttt tctaatactt gagctgcttc
     5581 ttctgcaatc tcatctgtat tttgaagtaa cgatactttg atcagttctc ttttttctaa
     5641 agcttcttca atttgaacga tcattgctga attgatccca ccttttccga tttgaaaaat
     5701 cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat
     5761 aattttcctt cttccttaaa tcaatgcttt gcgtttcaat acatctacac cctttggcgc
     5821 ccagcccgca attactactg gcgctgttac agtgatccag cctagaccgg caaaaacgat
     5881 gtctgttttt tctttcacag aaaactcaaa acgaacaagc tccggaaatg cctcgacttc
     5941 atcaggacga ggaggctgta ataatccacc tacatgtttt tgataaaatt cgtctgcttt
     6001 ttccagtttc gttcgatgaa tatttacatc atttgacaca taagcaataa atgaagcacg
     6061 attgccgctg ataaaatcaa atcttgcaag tccgcccaag aaaagtgttt gcccttcatt
     6121 cagctgataa actttaggtt tgatttcttt taacggtgca atgatccgca aatctttttt
     6181 acctaagtag tgcgccattt gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg
     6241 cccgtcatct aaaggaatct cgattttatc caaggtcgtt cctggaaact gcgaagttgt
     6301 gattacatct tgtactcccg ctgtattttt gatgatttga ttgataagcg tcgattttcc
     6361 gacatttgtt actccgacga tatagacatc ccgtccatcg cggtattctt cgattttttc
     6421 taatagttcc gtcatctcat gagatttctt agcactagtt aataatacat ctactggacg
     6481 aattcctgct tcgtaagcac gttccttcat ccattgtgtt aatttagttt ttttcaatga
     6541 tttaggtaat atatccactt tgttcccaac taaaagtact ggattgtcac cgacaaaacg
     6601 gtgtagtcct gggattagtg aaccattgaa gtcaaaaata tccaccacat tgacgatcaa
     6661 tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac
     6721 atcttgggtc tcattataat ggcgtaatct gaaacaacgc tgacaataga cttccccgga
     6781 agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt
     6841 ttgtataacg gcaccacagc cgatacattg taattcttct gtcattcgat tccacctcgc
     6901 cagcccatat cagggtgttt tgctaataga taggccataa ttcttttttc ccaaaaacga
     6961 ttaaattgtg tcttccaact atctgttgcg ataatgggtt ttacaaggat gctacgtatc
     7021 cctgcacgat tggctccacg tatatcagtc ataatctgat cgcctaccat tactatctca
     7081 tcatctgcta aatccaattg tttctttgct atgttgatcc ctcttgctaa aggtttcatt
     7141 gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta
     7201 ttgtttgaaa caacaacaac agggattcct gcatttttca tttctaaaat ccaagtaagc
     7261 aactcttctg ttccatctgg attattccat gcaatcaacg tattgtctaa atcggtcaat
     7321 actgctttga tgcctagatt ctttaattgg gcaggtgtaa tctgatagat ggcttctacc
     7381 atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag
     7441 cacataaagt actcctcaac cccgtattat actgcatatc agcaaaaaaa actacctcta
     7501 tactcgttcg tctttcagaa aaagcatttc tttacaaaat gaccaaaaat aaagattgat
     7561 cacagaaaaa tttagaaaca aaaaacaact aatattggcg gaacctcttg tatcgttttt
     7621 ccagtaattt atgctgatct aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt
     7681 tcataatgtg tgcgacttta tatcgtggca actgctcgcc gttgaacgtt tcaggaataa
     7741 ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt aattttcatc aactcagctg
     7801 cttctgcggc gcgactaccg tctttccaca aaatcgacgc aaatccttct ggagaaagca
     7861 cagcataaat cgtttcttcc agcatccaga cttcatccgc taaacctaga gcaagggcac
     7921 cgccacttcc accttcacca atgatgatgg cgatgatcgg tacctttaaa tcagacattt
     7981 cgagcagatt tctggcaata gcctctcctt cgccgcgctc ttctgctcct acaccgcaat
     8041 atgcacctgc tgtattgatc aaggtaatca ccggtctatt gaacttttcc gcttgtttca
     8101 tcagtcgtaa agcttttcga tagccttcag gatggggaga accaaaattt ctgcggatat
     8161 tttcctgcag atcccgtcct ttttgaatac cgatcactgt taccggtttg ccatctaatc
     8221 gagcgattcc accaacaatc gcttcgtcat ctgcaaataa acgatcacca tggcattcct
     8281 cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg
     8341 ctaactgaac gacatcatgt gctgtcttat ccacgatcta tccatccttt ctgtgtatgc
     8401 aattcaagaa gcgtatggat tttctgtttc aattccatgc ggggaacaat ctgatcgaca
     8461 aaaccatgag ataataagaa ttcagccttt tggaaatctt ctggaagttc ctgcttgatc
     8521 gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc tttggggttc tgctagaata
     8581 atatcgcctt ccattgcaaa actggcagtt actcctccgg tcgtcgggtc cgttaaaaca
     8641 gttagataaa aaagaccttc attgctatgc cgtttgacag cagcagagat ttttgccatc
     8701 tgcattagag aaaagattcc ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg
     8761 actggaagac tttcctttgt tgctcgttcg aataatctgg tgatcttttc tccgacaatc
     8821 gttcccatac ttcccatgat aaaattagga tccatcactc caataatgaa gggaatcccc
     8881 tgaatggttg ctttaccagt gaggactgct tcatgaagtc ctgttttatc ttgcattttt
     8941 ttaatttttt ccgtatattc gggaaatgct agcggatctt tggttacaag atccgtatcc
     9001 cattcttcaa aacttttttc atcaataatc aaagctagac gctgccatgc accgattcga
     9061 aaagcgtatc cgcaatgagg gcatactttt tcttcaccga tgtctttcgt ataaaggata
     9121 tgtttgcaat ttggacactt tgcccacatg ttatcaggaa cagatggcga gttagcaggt
     9181 ttgctctctc tattaggatt gatccgaata tatttctttt tcttaaacag agccatctat
     9241 gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct
     9301 gtgctgtaat cacctgctat gactgcagga tgactaatca aatccatctg aaattctgcg
     9361 ttcgtgatga ttccttctgt gataaattca cttaaggctc gctgcatctt cattaatgct
     9421 tcaaaacgcg tatttccatg gacgattatt tttgcaatca ttgaatcata atatggcggg
     9481 atcgtatatc ccgggtaaac tgcactgtct actcgcagtc ccatcccacc tgctggaagc
     9541 agtaagtttt ggatcttccc tggagaagga gcaaaatgga aagcaggatt ttctgcatta
     9601 atccggcatt cgattgcgtg tccttggaat actacttctt cttgacacac attcaaaggc
     9661 tcaccggctg ctatctccac ctgttttttt actaaatcga ttcctgtgac catttctgtt
     9721 actggatgtt ccacttggat tcgagtattc atttccatga aataaaaatc gccttcttca
     9781 tccatcagaa actcgatcgt tccggcattt tcataattga ctgctttagc tgctcgaaca
     9841 gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca ctacagaagg agattcttcc
     9901 agtacttttt gattgttgcg ttgtaaagaa caatctcgct cgcctaaatg aatgacatga
     9961 ccaaatcgat cacctaagat ctgaacttct atatggcgag caggataaat gattttttcc
    10021 aaatacatat catcatttcc aaatgccgct gctgcttctt gctgagctga agaaaaatgc
    10081 tgtgggagct cttcttttga ttgaactttc cgaatgcctt tcccgccacc acctgcagct
    10141 gctttcagca tcactggata accaattctg tctgctattt ccaatgcttc ttctactgta
    10201 gataatacac cagtacttcc tggaatgact gggacatttg cttcttgcat caattgacgc
    10261 gcattgatct tatttcccat tgcatcgatc gttgcacttt taggaccaat aaatgtgata
    10321 ttgcactctt cacacatgga ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga
    10381 tgaattgctt cggcttttgt cacgattgca gcgctcagta cttcctgaac attcagatat
    10441 gaatcaacgg cttttgccgg gccaatgcag attgcttcat ctgctaactg tgtatgaagt
    10501 gcttcggcat ctgcttctga atatatcgct acggtttgga tccctagttc gcggcatgca
    10561 cgaatgatac gtacagcgat ttcaccgcgg ttagctatca atactttaga aaacatattt
    10621 cacctatcca atcataaagg ttaattccgc ttcggctact tttttaccgt ctactttagc
    10681 gattcctttg cctattccgg ctgaagcttt cactttcaat atctcaactt caaggtataa
    10741 ggtatctccc ggtgtaactt tcttacggaa ttttgctttg tccaatccgc cgaaataagc
    10801 cgtctttccc ttgaattgtt caagagaaag taatgcaact gctcctgctt gagccattgc
    10861 ttcaacaatc aatacccctg gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg
    10921 ctcattgatc gttacatttt tcttagcaac gatccgttct ccttcaacca tttcttccac
    10981 tcgatcaatc aaaagcattg gataacgatg tggaatgatt tcttttattt cttgtatgtt
    11041 cattgttttc cctctttcct gctttgctta tttttgctga atcctaaaga gtggctgtcc
    11101 atattcgaca actgactcgt tttctacaag gatttctgtg atcactccgt cggcctcact
    11161 agttatctca ttcatcagtt tcatcgcttc aataatgcag ataacttctc ctttggcaac
    11221 ttgatcacct acttttttga aagctggttt gtcgggcgct ggttgcagat aaacgattcc
    11281 cacgattggc gaaacaacct cgattccatc aaatactgcc gattcttttt ctttcattat
    11341 ttctggagca gttgttgctt gttcaggtat gacattgact ggctccgaaa ctggcaacgt
    11401 ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta tttttattca tatacaattc
    11461 aaattgacct tcacgcaaat cgaattccgt tagcgaggat tgatcaaatt gactgactaa
    11521 ttctctgatt tcattgatat ccaaaattat tcctcccagc gtttcatgca cagaaccgca
    11581 ttatgtccgc caaaccctaa tgaattactc attgcataag taaaacttgc aggtttgctt
    11641 tcgtttttga caactgtcaa gtcaatctca ggatcttgtt tttctacatt gatcgtaggt
    11701 gggataaatt gatgttctaa tgccagcaaa gttgccaagg cttcgatccc gccggctgct
    11761 cccaataaat gtccagtcat cgatttcgtg ctgctgacgt aagtatctct gaaattttca
    11821 cctaatgcga tttggattgc ttttgcttct gcctggtcat tggctggtgt acttgttccg
    11881 tgagcattga tatatccgac ttgttccggc tgtacaccag cttcttccat cgcaagcttc
    11941 attgctttag atgcaccaga accatctgga gtaggagctg tcatatggta agcgtcacag
    12001 tttgcaccgt atccaacgat ttctccatag attttggcac cgcgagcttg tgcatgttct
    12061 aaagattcaa gaactagaac gcctgctcct tcacccatca caaaaccatt ccgattttcg
    12121 tcaaacggaa tcgaaccacg attaggatca gttgattctg agagtgctgt taaagctgca
    12181 aatccagcaa tccccatctc acaaatcgtt gcttctgttc ctccagcaag aatgacatca
    12241 gaatagccat gtttgatgtt acggaatgct tccccaattg aattcgttcc gctagcacaa
    12301 gctgttacga tagctgtaca aatacctttt gcacctacac gcaaagcaat atttccagct
    12361 gccatgttgg caatcgccat tggtacgaac attggagaaa ctcgttttgc acctttttca
    12421 tgtagacgaa taacttggtt ttcgattgtt ggaagaccac cgatcccaga tccgatgatg
    12481 acgccaaacc ggtcttgatt cattttttct ttatctaaac cgctcatctc cattgcttct
    12541 aaagcagcgt agattccata gatcgaaaac atatccattc gtttgctatc ttttttaata
    12601 aaatatttat cgtaagggaa atctttgact tcacccgcta aggtaatccc tgtttctgaa
    12661 gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct cttttaagct attcaaaaag
    12721 ctgtctgcgt catttccaat aggtgaagtg actccatagc cagtaattac cactcgattc
    12781 atttgtttgc tctccttttc ttatccgtgc atgaccaatc cgccatcgac attgatgact
    12841 tgtccggtga tatatggatt ttgtgctaag aaaactgctg tttgtgcaat atcttctact
    12901 tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa
    12961 acttccgtca tatcagttgt gatgaatcct ggcgcaatgg cattacatgt aatgcctcta
    13021 gttgcagctt ctcgtgccac agattttgtt aatcctacta cgccagcttt actagctgca
    13081 taatttgctt gaccaatatt tccaatcaac ccagatacac tagagagatt aatgatggcc
    13141 ccttctcgct gcttcatcat ttttttcaat acgtgctgcg tcatattgaa ggttccaatc
    13201 aaattgatat ccagacattt tttgaaatct tctgcatcca ttcgcatcac aagtttgtca
    13261 tttgtgattc cggcattgtt tactaacaca tggatcgatc caagcttttc ttctgcttct
    13321 ttgatcattt gtcccgcttt ctcataatca gagatgtcac cggaaactcc tacgcatttt
    13381 acaccgaatg cttcgatttc ttctatcttt tcttttggaa tctctccgcg accattcaaa
    13441 atgatatttg ctccagcttt tgcaaaagct aaggccatcg ctttgccgat tcctcttgta
    13501 ctacctgtga taaatacatt ttttcctttt acatccataa cgttcctcct tatagtattg
    13561 ctattgtttc atcaaatgtc tgcttattct caacacgcag aactggtatt gttttatcga
    13621 tttttttcat aaatccgctt aatacttttc cggggccgac ttcgatgact tgttcgatgc
    13681 cgattgtttt taacttatgg atactctcat agaaacgaac aggttgcatg acttgctgtt
    13741 caagtagtcc cgcgattgtt tctttcttca ttatttccgt tgttgtgttg cttatgatag
    13801 gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt cgccagtttt tttgctgccg
    13861 gttctaaaat agctgtatga aaaggcccgc tgacatttag agggatcatt cttttgaacc
    13921 ctttttcttt taatagtaaa acagcttcgt ctactgcttt ttcttcgcca ccaatcacga
    13981 tctgctgagg tgtattataa tttgcaggag atacgatgcc gtacttgcta gcttcatgac
    14041 agctttcctc gatcgtttca atcggcgcat tcatcactgc aaccattttc ccgcttccag
    14101 ctggggcagc ttctgtcata taagcgcctc tttttgctac taacgcaact gcttcagtga
    14161 aggatagcgc attactagct actaatgccg aatattctcc taaactcagt cctaaagctg
    14221 catctgggat gattccatgc tccttcaata agcgataata cgcgatgctg actgttagaa
    14281 tagctggctg tgtatattgc gtctgatcca aacgttcatt ttctgtaaag caaagttcag
    14341 ccatatcgta gccgagaatc tcacttgctt catcaaatgt ctgctttaca acggcttctt
    14401 catataaatc ttttcccata ccttgatatt gtgcaccttg gccactaaat aagaatgctg
    14461 ttttcatgtt tttctccaat atatccagaa tatatgtttg attcttccta ttcaaaaagg
    14521 ccgagacatt cctatttcag gaaatgtccc agcccccaac ctaattttaa tgattatgca
    14581 gtttgttttt ctacgtaagt cactaaatct tgaaccgttt tgattccgtc ttctgtttcg
    14641 attttcacgt caaattcatc ttcaatttca ttgatgattt ggaataaatc caagctgtca
    14701 gcttctaagt cttcttggat gtcagtagtc aatttgattt cttcagtttc ttttcctaat
    14761 tcttctgcga tgatttcttg aattttttcg aataccataa taatatattc ctccgatatt
    14821 gtttgctttt tatttttata atttaacagt ctacaactcc atgagaatac ttccccatgt
    14881 taaacctccg ccatagcctg taaatattac tttctgctgt tttcctaagg tgatctttcc
    14941 actagaaact gcttcatcca ataagatagg gatgcttgca gcagatgtat tcccatattt
    15001 atccatattg gtcaggaact tttcttgcgg catctttact tttcgggcaa ttttctcaat
    15061 aattctgagg tttgcctgat gcaaaagcag ataatcaact ggtttgtctt tcatgacttc
    15121 ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg
    15181 caaataatac gaaccttctg aatcttctga atagaacgga tttttatttt gctggtagcc
    15241 agcggtcaat gagctacttc tggtaccgtc agctgctaat ttttctttca gcaatttttt
    15301 ctgtggattc gcttctaaga gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt
    15361 acgatcagac caatcgatta gcttagatag tgtttcacca ccaatcacca gtcccttagt
    15421 tgagccgcag cggatcattt tttcagccat gcttaacgca taaacaaatc ctgagcacgc
    15481 agcactgata tcaaaagcga tagcatgagt tgctcccaat ttcccttgta cttgtgctgc
    15541 tacagatggt gtcgtgtaat cgggcgacat tgttgctact aaaataaaat ccaaatcttt
    15601 agggtttaga tttttctgtt ttatcaattt ttccgccact aaaatgcata aatcagaagt
    15661 ctcctgattt gttacacatc ttcgttcacg aatacccgtc cttgacgaaa tccattcatc
    15721 agaggtatcc atcatcttgg aaagatcatt attagtaata atcttttcag gaaggacgct
    15781 ggctgttgca gtaatcattc caaattgttc cattaaatat cctcacttat agtcttgcaa
    15841 gaagtcgtgc aagtttttca gtgcatccag caaggcatgc tgttcctccg tagccattcc
    15901 gtctaagatg ttcttgacca tttcacgatg aaaatgctga tggacccgaa atagtaactt
    15961 tccttttttc gttagtccca actttacaac gcggcggtca tcttcactgc ggatacgttc
    16021 gacatagcct ttttttacta acttgttaat agcagtggtc aaggtcccaa cagtaattga
    16081 tagttctttg gctacttctg aagttgtttt tttcttgtac atcccgattg cctcgatcgt
    16141 gtgcatctct gtgattgaca gatctttgaa ttgagatttt ttcagctcag actcttcgat
    16201 tgtcaaaata tcattgaaga cactaacaag ataatcattt actgttttta aatctggttc
    16261 catgttccac cgtccactaa ttagtttgat tatcaaatct tttgatgttc aaactatatc
    16321 ttaacttttt tcaaaatgca accttaaaag atcatttttt tactatatga gcttttttta
    16381 agattttact catttaaaac aactgataaa tcagcattta taatttcttt tattagaaaa
    16441 atcatctatc tcacatattt tgattatcaa agtattttct gtgaacagaa ctatagaatt
    16501 ccctaaaact tgtccattat ttcaaaattt gttatagtct ttttattcaa agcgtaaaag
    16561 gagatcctat gttaaaagaa aaaatgaaaa ctctccccat gctgccgggt gtctatctta
    16621 tgaaagaccg agatggtaag gtcatctatg ttggtaaagc aaaacggctc aaaaatagag
    16681 taagcagcta ttttcatcag aataaacagc attcaaaaaa agtattacgt atgattcatc
    16741 atataacaga ttttgatttc gtcgtagtag acacggaatt agatgcttta ttgctcgagt
    16801 gtcaattgat ccaacattac cgtccttttt ataaccgaca aatgaattat ttcagtaatt
    16861 ataattatgt acatattacc aataagggat ttgtattaac agatactcct acggctcgaa
    16921 catacgggcc ttttcgtctc tataaaaaaa tgccgtctat tttgcgtata atggaagaaa
    16981 cttatcaaat gccttggttg tctgaaatta gtttgttagc tttgcgagtc cagcttcctg
    17041 acctacagga aatgactttt gaacagaaaa aaaaggaact ccaagggctg tttcaaggcc
    17101 gaaataaaaa gctactgact tacttgaaaa aaagacaaca acatttcatc tatcagttga
    17161 atttcgaaaa agccggtatg cttcaaaaag atatcgaatt agttacttat tttattagac
    17221 ggatccagga acaaaagcag tttttaagaa caccatcgct caccttcagc atgccattag
    17281 cagcagatga gagtcagaaa aagcactacc tcgtttgtta tggtcaactt gcagaaacaa
    17341 tgattacctc tggtgacgtt tctcctgact tctattatga aaagaaagaa gctcatttat
    17401 ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt acaaatcttg atcagttacc
    17461 gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt aaacaaaaaa gaagctgaaa
    17521 agcaactaaa ttagttgctt ctcggcttct tttaggcatc ttcaacttca aatgtcaatt
    17581 atcctaaaat ttattggatt aagtcataga tttccatcgc tacgatatcg atattatcaa
    17641 attcgtattg ctgatgagta tctgtttcag ttaattcaaa tgtatcggtc gctttatcat
    17701 aagttactgt acacttctct acgccttctt tttcaaaacg gcgaacttgc agttcattat
    17761 ctgctgactc gaccatagcc tccagtcgtt ttatgattgc gactaattgt gaatgtgtca
    17821 tcggaggtcc tccttttctt atttctgtta tattgtatca aaagcatagc ctacttgcat
    17881 tgattttctc taaaaaaact cgttttttat gatttttttt ctggtatttt tccttcaggc
    17941 caccaaagtt tgatcaaagc ttgggttccg tcattctcaa aacctttatc tgccaactct
    18001 tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa gatccatttt ttctgcttct
    18061 tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa aaaaaccagg tgtgtaatct
    18121 tcttttaaaa tacgaggaga ataattgctt aaagaccagt tagctgcact tccacctgaa
    18181 agcgtctcaa tcactttctt taaatcaagt ccgttttttt gtgcatagac aagcatttcc
    18241 gtcattcctg tcatcgtacc tgcaatcatg atctgattag ccatttttgt atgctgacct
    18301 ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt ctttgaataa aggcagtact
    18361 tgatcataaa cggcttcttc tccaccgacc atgatcgtca gtgtaccatt ctttgctcct
    18421 aagtcaccac cggataccgg tgcatctaac gcatctgcgc ctttttcttt tgctgttttt
    18481 gcaatttttt ctgccaggct tggtgtactt gtagtcaaat caactaaaat atgtcctgag
    18541 atatctgcag aaaaaatccc cgactgtcca tagtagattt cttctacatc tttggggtaa
    18601 cctaccattg taaagagaat attactttgt tctgctaccg cttttggtgt atctgcccag
    18661 atagctcctt ctgttactaa aggatcagct tttgcttttg tccgattata gacagctact
    18721 tcgtgtcctg cttctagtag atggcgtgcg acagcacttc ccatgacgcc tgttccgata
    18781 aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa tccttgcttc tatgctagta
    18841 tacgctagaa acaaggattt tttcataaaa aatgtttgaa ttttatcttt taaatgaatt
    18901 taattttatt ttgattcgtt caatcgagtc ttcttcttct tttcttcgca tctcttcttc
    18961 atgataaaca accatgtctt gataaaattc gatcatagct tcgccgaaat gtgtagatga
    19021 aatatcataa agtttagctt ctaaaacttt tggatcttta ggaacttcac tggaaatata
    19081 atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa gttttcccta atgaaggatg
    19141 atcgaacaat cggttcaaat aagcatttcc ttccacgaca caaggtgttc cggctgccat
    19201 cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa gcactgacaa aataatctgc
    19261 cgctttataa taaagtgcta catcattgtt gtccacttct cctgtgaact gcacaaattc
    19321 ttctaactgc agttctgaga tcaattcctc taactcttct acataaggtc cttttcctac
    19381 gataactaat ctggcgttag gataagcgga gatgacctga ggaaacccat taatcaaggc
    19441 ttggatattt ttctcataag aaatcctgct taaagaaaga atcatcaaat ggttttcctg
    19501 aagacctagt gactccctta atttgctgat atcctgctga gtgatatcag aacgtttgaa
    19561 tttttcaatg tcgataccag taggaatcac gcgaagcggt gctattacac catagctgcg
    19621 taatgtatca ataacacgtt cacttggaca aactacacct gtcgtatgat ttgcaaataa
    19681 tctagacaag tattttacat gggattgcct gatcactttt cctttaccga tataatgaag
    19741 gtagtcttca tacatcgtat ggtaagtatg gatcagaggt attttcaact tcttagctac
    19801 catcttccca agtatccctg ctccgaattc tgtatgggta tgaataagat ctagttctag
    19861 ttctttggca accaaataag cataccacat gcctctaaca acaactcttc gatccttgaa
    19921 agaaataaat ggtacacttg gcattcgaat cacatctttt tccaacttat ccgcattagg
    19981 atcggtcgtt gtaaaaatat agacttcatg cccatgtctt tctaattctt gttttaatgt
    20041 tttgatcgat gtagcaactc cgctgacttg cggaaagtag gtatcggtaa aaaagccgat
    20101 tttcatgttt ttcgcctcca aactagtcct tgacctgatt tacttttttt aatttgctag
    20161 agactttttc attcagtaca ctttcgtata catttttaag ttcttcaccg attttattga
    20221 tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt actcggtagt tcttgattgg
    20281 taatctgttg gatcagtcgg ataaattctt cgtttgtctt ccccatatag cagttcttgc
    20341 tatcttctaa ccagccatta tacactggaa tatctcgaac aagtacgttt tgtttgcttg
    20401 ctaaagcttc taaaacaacg atcccttctg tctcttcata agaagggaaa aagaatagat
    20461 ctgcacctga atacgcacct tctatgatct ctccacgaat gtaaccagga aacaggacat
    20521 tttcaggatg atcttctttt acaattttcc ggatgttccg aggaatcgag tacatgggaa
    20581 catgaccaaa ccaaataaat gtgtattcgg gcatcttctt agcaatttct acgaaatcaa
    20641 tgattccttt acgttcgaaa aacaaaccga cacaaatgat tactttttga tccggagaca
    20701 gtttgaaata ttctctgaac ttttgttctt tcattggatc agggtgatat ttctctaagt
    20761 cgatcccatt ggaaatagct tgaatcggca cacggatacc atagctttcc aacaactgtt
    20821 ttgaataagg tgttggcgtg atcagatagt ccgctttttg gtacaatcct accagatatt
    20881 tttttacgat aggagaaagc tggttcgatc tgataaagga atttcggaaa tcttcctctg
    20941 tagaatgcgc atgatagaca actttttttc cttctcttct ggctttattg accatattat
    21001 gactattgat tccgtatgta ttgatgtgca ggatgtcata atcttctttt tcatctagtg
    21061 tataagagat accgacttcc gtcagagcac gtttttgatg atcaagcgca cgcccgattc
    21121 cagattttgc aagcatcttt tcactttcaa aatataataa taccttcacc ttacgtcccc
    21181 cttcacttat atagtataac atagcctaca aactgtcact caaacttaag ccctattttt
    21241 gtctattctt actgacaaaa acgttttctt aaaaaagaac aaaaaaactg gcgtagctct
    21301 tttacaagcc acaccagctt ttgatacgtt gtttatttta catattcatg aacaagttca
    21361 acgacttctt ccatcatatc gcattctttc aatgcacgat cagcaagttc agccattttc
    21421 gctgtgtcca agcgtttcat taaactacgc gttttaagaa tagatgtcgc actcattgag
    21481 aactcatcta gacccatacc tactagcaat ggaacagctg tttgatctcc agccatttct
    21541 ccacacatac ctgcccattt tccttcagcg tgtgctgcat caattacatt tttgatcaaa
    21601 cgtaaaattg atgggttata tggttggtac aagtaagaaa cgcgttcgtt catacggtct
    21661 gctgccattg tgtattgaat caaatcgttc gtacctacag agaagaaatc tacttctttt
    21721 gcaaacttgt ctgctaagac tgctgctgct ggaatctcga tcatgatgcc gacttggatc
    21781 gtatcagata cttccttgcc ttcacttatc aatttttgtt tttcttcttc aaagatcgct
    21841 tttgctgcgc ggaattcttt gagtgtagct accataggga acatgatacg taaattacca
    21901 tgaacagatg cacgaagcaa tgcacgcatt tgtgtacgga acatttcgtc gccttgttca
    21961 gataaactga tacgcaatgc acgatatcct aagaatggat tcatttcatg aggtaattgc
    22021 agatatggta gttctttatc tccaccgatg tccattgtac gaacaactac aggttttcct
    22081 tccatacctt ctaatacagc tttgtaagct tcatattggt catcttctgt tgggaagtct
    22141 ggagaatcca tatacaggaa ttcagtacgg tacagaccaa ctgcttctcc accattatta
    22201 tggacaccaa ctagatcttt tggtgttccg atgtttgcag ctaattcaaa atgcttgccg
    22261 tcagctgtga cagtttctgc attttttaat ttttcccatt ctgctttttg tgctgcgtaa
    22321 tctgcacctg ctttttcaaa ttcagctttt tgttcatctg taggatcgat gattacatcg
    22381 ccttcgatcc cgttgacagc caaaatatcg ccttctttga ctttagcagt aatttctttt
    22441 gtcccaacga ttgctggaat ttctaaagaa cgtgccatga tagcagaatg tgaagtacgt
    22501 ccaccaatgt ctgttacaaa cgcttttaca aaattgcgat ctaattgtgc tgtatcactt
    22561 ggagtcaagt catgtgcaac aacgaccact tcttcgttga tcattgatgg atttggtaaa
    22621 gtaacgccta ataagtgcgc taaaatacgt ttagcaacgt cacgaatatc tgcagcacgc
    22681 tcttgcatat aagcattatc ttccattgct tcgaacatac cgatatacat atccgttact
    22741 tcttttagcg ctgattcagc atttacgctg ttgtctttaa tattttgctt gatttgacct
    22801 accatttctg gatctgaaag aaccatcaaa tgggcatcaa atacttgcgc ttctgcttca
    22861 cctaagcttt gcgctgcttt ttcacgaatt tgctgcaatt cttcagtaga tttagctaat
    22921 gcagcatcca aacgagcttc ttcagccgca gtatcttcta ctgttgcctt actgaatgtt
    22981 aaatccggtt gaactagcag gtaagctttt gcaacggcta caccgtcact agcggcaatc
    23041 ccttttagca tttcaaccat tattcagata agccttcttt cttcattgtt tcaacgattg
    23101 ctgccattgc atcagcttcg tcagcacctt ctgcagtaat agtaacatca gaaccttgac
    23161 ctacacctaa agacataacg cccatgattg atttcaagtt tactgattta cctttatact
    23221 ctaagtttac atcagagtta aatttgcttg ctgtttgtac taatagtgta gctggacgag
    23281 cgtggatccc agtttctgct actacgtgaa attctttctt ttccatatta atcggtctcc
    23341 tttaagtgag tgttgttttt atgtgagggt tttctttttt agtaattcag agaatcctaa
    23401 caaatgccac cctttcatct gtaaagatta acattttttt tcgttagata caacagtttt
    23461 cattcaattt cagataaaag tattcaaatt attccggatt ctttttcatc cttttcttca
    23521 cattttataa caaacgtgtt tatttccttt ttcttgtcgc tttctcttct cctgacgttt
    23581 atcattcttt cctttgaccc tctttgtttt catatgaaac gaattacttg cttccctttt
    23641 tatttttagt ataataagag tcaaaggtca ggaatggtca atagagattt caccttttct
    23701 acctcataaa tctcttaaaa aactaggggt tattctttta cctcatgaaa aacgagatgt
    23761 aaaatatgga tgtacccatt tttgtgaaag gacgtgaatg attcatgctt tgtcaaaact
    23821 gcggcaagaa tgaggcaacg attcacttgt atgccactgt caatggtcag cggacacaac
    23881 tcgattactg tcagagctgt tatcaaaaaa tcaaaaatca acaaaatgga ggtctgacta
    23941 atatggccca aaatgatcca tttggatttg gcagtttgga cgatcttttc cgttccatgt
    24001 ctcgccaaat gcaacagcaa ggaaattatg agcaaacacc accaacacaa tttggcggaa
    24061 acaacaactt caatggtggt cagccacctc aaggcaatac agaaggttta ttgggagaat
    24121 acggaatcaa tatcacagaa caagcacgac aaggtgatat cgatcctgtg atcggacgcg
    24181 atgacgaaat caaacgagtc attgaaattt taaatcgccg aaccaagaac aatcctgtct
    24241 tgattggaga accaggtgtt ggtaaaacag cagttgttga aggtttagca caaaaaatcg
    24301 tagatggtga tgtaccgcaa aaattaatgg acaaagaagt cattcgttta gatgtcgtat
    24361 ctttagtcca aggtactggt atacgaggac aatttgaaga acgtatgcaa aaattgattg
    24421 aagagatccg tcaagcagaa aatgtgatcc tattcataga tgaagttcac gaaatcgtcg
    24481 gtgctggttc tgctggtgat ggaaatatgg atgctggtaa tattttaaaa ccagctttag
    24541 ctcgaggaga attgcaaatg gttggtgcta ctaccttaaa tgagtaccgc atcatcgaaa
    24601 aagacgctgc tttagaacgt cgtatgcaac ctgtacgggt agatgaacca actgtcgaag
    24661 aaacgatcag tattttgaaa ggtttgcaaa aacgttatga agactatcat catgtaaaat
    24721 atactgatga ggccatcgaa gcagccgcaa ctttatccaa tcgttacgtc caagatcgct
    24781 tcttgccgga taaagccatc gatttgctag atgaaacagg ttctaagaaa aacttgacga
    24841 tccaaatcgt tgatcctaaa acaatcgaga aaaaactgca agaagcagaa gaacaaaaag
    24901 ttttagcttc tcgagaagaa gatttcgaaa aagcagctta ttatcgcgat cagatcaata
    24961 aacttcaaaa aatgaaagaa cgtcagctca ctgaagagga aacacctgtg attaccgaaa
    25021 aagatatgga aaaaatcgtt gaacaacgga caggtattcc agtgggtgaa ttaaaagaaa
    25081 aagaacagac acaactgaaa aacttagcag atgacttgaa agctcatgtc atcggacaag
    25141 ataacgctgt agatcgagta gcgaaagcga ttcgccgaaa ccgtgttgga ttaaacaaac
    25201 agaatcgtcc aatcggctcc ttcttatttg ttggaccaac tggtgtaggt aaaacagagc
    25261 ttgcgaaaca acttgcctac gaactattcg gttcacaaga ttcaatgatt cgtttcgata
    25321 tgtctgaata tatggaaaaa catagtgtct ctaaattgat tggttctcct ccaggttatg
    25381 tcggatacga agaagctggt caattaacag aaaaagtacg gagaaatcct tacagtcttg
    25441 tcttgttaga tgaagtagaa aaagctcatc cagatgtttt gcatatgttc ttgcaaatct
    25501 tagatgatgg tcgattgaca gatgcacaag gaagaacggt cagctttaaa gatacgatca
    25561 tcattatgac aagtaatgcc ggaactggta aagtagaagc aaacgttggt tttggtgcgg
    25621 cacgcgaagg tgttactcgt tctgtattga accaattaaa caactacttt acaccagaat
    25681 ttctgaaccg ttttgacggg atcatcgaat tcagtgcatt atcaaaagaa aacttgatga
    25741 caatcgtcac actaatgcta gacgatgtca accaaatgct ggcagcacag caacttcaca
    25801 tcgaagttcc tacgaatgtc aaagaaaaat tagttgactt aggctatgat ccatctatgg
    25861 gtgctcgtcc tcttcgacgc acgatccaag aacaaatcga agatggtatc gctgaatttt
    25921 atcttgatca cccttctatc catgagttga aagcaaaact ggataaagat ggaaaaatca
    25981 tcgttacttc taaaccagaa cgtttggcta aagaatcagc cgaagaaacg gctgaataaa
    26041 tccagtttta tagcgtgaga cggtatttct ttgagaaatg ccgtctcatt cttttttcag
    26101 aaaagtcaaa atactgttca ttttataaaa aagaaattat aataagagaa acaagcaatt
    26161 aaaaatacat aaaagaggtg agcgccatga aattggtaaa tgtaacaaac agccattccc
    26221 gtcttgtatt aaaccagctt gaaaacactg atgcccatct agtcaaggtc tacacagccg
    26281 gaaataccac tattgtctac actgaagccc ctgaacataa tgaaattctg ctgatcaatg
    26341 acaaacgtaa aatccagccg aaagaaatcg aagatgctaa aaattatttc ttagataaaa
    26401 tcaaagacgc cgtttaccac gaggatgaaa tcaaagtaat cgaattggca ggccttgttg
    26461 aaatctcgat tccaaaacat gaattaggcg ctacattagc cgtgtaaaca aattttgaac
    26521 ataataatta aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    26581 gtataaacgg cgaaagcaga agcaactcct tcggaaataa gctgaaattc acgaaaattg
    26641 cttttcaaat ttttgtgaat tccagcttat ttcttggagt taaacgcttc tgtctcagcc
    26701 tctttttctt tctagctttt tgatttttct cttttctttt gtacttttct cttttcattt
    26761 tcagttctac gcgtgtcttt atttttaact ttttcctctt tatggatgat tgatcgtact
    26821 ttttgtgcaa tttgctcgtt tttgtcccag tcttttacat ctaataatcg acataatcgt
    26881 aacatctctt cttttttctg tttttttctt tcacgtcttt gccattctgt ttccatgtgt
    26941 cacactctcc tttctttaat catatgtacg caaaataatt tttatatttt tttgattaga
    27001 catttctatg attttaatat agtaaaaaga gccgtcacgg aaaatc
//
LOCUS       DAZKAO010000026        26789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000026 DAZKAO010000000
VERSION     DAZKAO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.026"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..494
                     /locus_tag="ACG8H8_000895"
     CDS             117..494
                     /locus_tag="ACG8H8_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985985.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            1063..2451
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000896"
     CDS             1063..2451
                     /gene="ltrA"
                     /locus_tag="ACG8H8_000896"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP8985986.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2573..3868)
                     /gene="purB"
                     /locus_tag="ACG8H8_000897"
     CDS             complement(2573..3868)
                     /gene="purB"
                     /locus_tag="ACG8H8_000897"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP8985987.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3881..5005)
                     /gene="purK"
                     /locus_tag="ACG8H8_000898"
     CDS             complement(3881..5005)
                     /gene="purK"
                     /locus_tag="ACG8H8_000898"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP8985988.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4998..5486)
                     /gene="purE"
                     /locus_tag="ACG8H8_000899"
     CDS             complement(4998..5486)
                     /gene="purE"
                     /locus_tag="ACG8H8_000899"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP8985989.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5902..7209)
                     /locus_tag="ACG8H8_000900"
     CDS             complement(5902..7209)
                     /locus_tag="ACG8H8_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP8985990.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(7212..7793)
                     /locus_tag="ACG8H8_000901"
     CDS             complement(7212..7793)
                     /locus_tag="ACG8H8_000901"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP8985991.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7909..8006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(8365..9657)
                     /locus_tag="ACG8H8_000902"
     CDS             complement(8365..9657)
                     /locus_tag="ACG8H8_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8985992.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9654..10277)
                     /locus_tag="ACG8H8_000903"
     CDS             complement(9654..10277)
                     /locus_tag="ACG8H8_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985993.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(10280..11116)
                     /locus_tag="ACG8H8_000904"
     CDS             complement(10280..11116)
                     /locus_tag="ACG8H8_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985994.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(11133..12020)
                     /locus_tag="ACG8H8_000905"
     CDS             complement(11133..12020)
                     /locus_tag="ACG8H8_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8985995.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(12036..13319)
                     /locus_tag="ACG8H8_000906"
     CDS             complement(12036..13319)
                     /locus_tag="ACG8H8_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8985996.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(14002..14685)
                     /locus_tag="ACG8H8_000907"
     CDS             complement(14002..14685)
                     /locus_tag="ACG8H8_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP8985997.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14703..15320)
                     /locus_tag="ACG8H8_000908"
     CDS             complement(14703..15320)
                     /locus_tag="ACG8H8_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP8985998.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(15330..16154)
                     /locus_tag="ACG8H8_000909"
     CDS             complement(15330..16154)
                     /locus_tag="ACG8H8_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP8985999.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(16147..16707)
                     /locus_tag="ACG8H8_000910"
     CDS             complement(16147..16707)
                     /locus_tag="ACG8H8_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986000.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16881..17837
                     /locus_tag="ACG8H8_000911"
     CDS             16881..17837
                     /locus_tag="ACG8H8_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8986001.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(18142..19098)
                     /locus_tag="ACG8H8_000912"
     CDS             complement(18142..19098)
                     /locus_tag="ACG8H8_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP8986002.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            19202..19804
                     /locus_tag="ACG8H8_000913"
     CDS             19202..19804
                     /locus_tag="ACG8H8_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8986003.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(19892..21391)
                     /locus_tag="ACG8H8_000914"
     CDS             complement(19892..21391)
                     /locus_tag="ACG8H8_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP8986004.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(21407..21931)
                     /locus_tag="ACG8H8_000915"
     CDS             complement(21407..21931)
                     /locus_tag="ACG8H8_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP8986005.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            22229..22978
                     /locus_tag="ACG8H8_000916"
     CDS             22229..22978
                     /locus_tag="ACG8H8_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP8986006.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            22969..23754
                     /locus_tag="ACG8H8_000917"
     CDS             22969..23754
                     /locus_tag="ACG8H8_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8986007.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            23723..24439
                     /locus_tag="ACG8H8_000918"
     CDS             23723..24439
                     /locus_tag="ACG8H8_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8986008.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(24541..25560)
                     /locus_tag="ACG8H8_000919"
     CDS             complement(24541..25560)
                     /locus_tag="ACG8H8_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP8986009.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(25579..26580)
                     /locus_tag="ACG8H8_000920"
     CDS             complement(25579..26580)
                     /locus_tag="ACG8H8_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP8986010.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         8064 a         5688 c         4789 g         8248 t
ORIGIN      
        1 tgttttatca gaaacgaaaa tcttatacca tcaacaagcg tttacattta tacttaatga
       61 tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac ttgccgatgg
      121 aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa gaacgttcct
      181 gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa gatactagtt
      241 tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc cgtaaaagga
      301 cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt catgaacaga
      361 agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag atggaatacg
      421 cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat aatagccaag
      481 ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa gaggtagccg
      541 cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa gaagcagaac
      601 cgctaacgga tctgcttctt ttttggtgtg cgccctgcat gggtgaaaac ttggtggtga
      661 aagtccacta caggcttggc agtaggaact gttagcgaaa gacaaggcgg caatcgtgag
      721 gttgtggctg aaggaagtcg gacgcaaact cctgaactga cgaacagaaa ctagatacaa
      781 ggctgaatta ggtcggataa gtcttcatca caagacaaag tccaatactg cccgaaacct
      841 atacagtaaa tctagcagtt acatgggagg aaagttttca ctcttaccgg gggagatctc
      901 atgagggtgt tgtgggcgtt gaatgaaaac aaaccagcac aacgatcatg gcgacatgat
      961 ggtgaatcat gagaagtcag ccgaggtcat agtagtaacg acactgaccg ttacgaagga
     1021 ctgaacaata agaatcttga ataatttagg aggtgtgaga agatgcgacg accgcagaaa
     1081 acaagaaaac ttggctatca tcaaagagct agtgtggaac acgaagggta tgatgaagtg
     1141 cgcagcactt cttgcggtga acagaggaaa caagatggtg tagaaaatct gtttgggaga
     1201 attctagatc ggaataatct caatcaagcg tatcttcaag tagtcagaaa taaaggggcg
     1261 gctggtgcag atgggatgac cgtcgatcaa ctgcttcctt acttgaaaga gcaccgcgag
     1321 gaattactat ccaatttacg ccaagggaac tataaacctc agcctgtttt gcgagtagaa
     1381 ataccgaaac caaacggcgg tgtaagaaaa ctaggaattc ccacggttat tgatcgtatg
     1441 cttcaacagg caatcaatca agtgttacaa ccgctatttg aaccagaatt ctcagataat
     1501 agttatgggt ttcgcccaaa tcgcagcgca catgacgcag tgaagaaagc aaaatcttat
     1561 tatgaacaag gttaccgata tgtggtggac attgatatga aagcctactt tgacaccgta
     1621 aatcacgata agctgatgta tttcattgag cggaaagtaa aggacaaaca agtcctaaaa
     1681 ctgatccgtc aatacttaat gagtggtgta atggaaaatg gactcatcac aacaactgaa
     1741 gaaggaacgc cccaaggagg caatctctct cccctgttaa gtaacatcta cctgaacgaa
     1801 tttgatcaag tgctagaaaa tctaggacat aaatttgttc gatacgccga tgactgcaat
     1861 atctatgtta gaagcaaacg agcaggacag cgtgtgatga agaaatccat tcaatttctc
     1921 gaagaacaac taaagttgac ggtaaatcga gaaaagagtg cggtaggaag tccattgaaa
     1981 cgaaagtttc ttggattctg cattctgcca acaaagaaag gtattaaaat tcgccctcac
     2041 gcaaaagcaa aacagcgtgt gaaaagcaag ctcaaacaac tcactaaaag aaaccgtgga
     2101 agaagtatcc aacttatttt aaaagaaatc aagcaactaa tgactggttg gatcaactac
     2161 tatggaattg gtgagatgaa agaattcatg agggagttaa acggttggtt gaagcgaaga
     2221 atcagacaat atatttggaa acaatggaag aatccaagaa ccaaaagaaa aaatctgatt
     2281 cgtttaggga tcgataaagc gaaagcgtat gaatggtcaa atacaagaaa aggtatttgg
     2341 tcagtctcca aaagtcatat tcttcatcga tcattaacag acaaagaact ggcccgccaa
     2401 ggatatgagg acatctcctt gaagtaccag ttcgtacact caacttattg aaccgccgta
     2461 tacgggtccg tacgtacggt ggtgtgagag gggcggaagg tcagttggct ttcccctcta
     2521 ctcgattatt tttgctattc tattttaatt gaattttgca actcactctt ctttattcca
     2581 aacctactcg tttgaatatc acgtcaacgt ttttcaaatg atgatgataa tcaaaggcat
     2641 cgtctatttc ttctttagat aataccgatg tgatggttgg atcttcttct aacagcgggc
     2701 ggaactgcac ttggttgtcc caagcgtacg cagtttttgg ctgaacaaga tcataagctt
     2761 cttctcttgt cattccctta tcgatcaatt tcagcataac gcgctggcta taaatcaaac
     2821 caaatgttgc agacatattc cgcttcatgt tttcaggaaa gaccgttaga tttttaacga
     2881 tattgccaaa acgatttaac atgtaattca ataaaatcgt ggtatcaggc aagatgattc
     2941 gttctgctga agaatgagaa atatctcttt catgccataa tgtgacattt tcataggcag
     3001 tgaccatgtg tccgcgcacc actctggcta gaccagccat attttctgaa ccgatcggat
     3061 tccttttgtg cggcatagca gaagaacctt tttgaccttt cgcaaaaaat tcttctactt
     3121 cacgtgtttc ggatttttgc aatccgcgta tctctgttgc aaaacgttca atacttgtag
     3181 caataagcgc catcgctgag aaatattcag catgtagatc ccgagggaga acttgagtag
     3241 agatttcttg tggacggatc cccaattgtt tacatacata ctcttcaata aatggcggga
     3301 tatttgcgaa cgttcccact gctcctgaga tttttcctgc ttctactcct tttgccgcat
     3361 gttcaaatcg ttcgatgttc cgtttcattt ctgaatacca tgtggcaagt ttcaatccaa
     3421 aagtagttgg ttcagcatgg acaccgtgtg tccgacccat catcactgta tatttgtgtt
     3481 cttttgcttt ttcgccaatg atttccgtaa aacgtttcaa gtcttcccgt aaaatatcat
     3541 ttgcttgctt gatgagatag ccataagcag tatctacgac atccgtactt gttagaccat
     3601 aatggaccca ttttctttct tctcccaatg tttcagaaac cgctcgtgta aacgcaacga
     3661 catcatggcg tgttaatttt tcgatttcca agatccgatc gatatcaaat gatgcatgct
     3721 cacgaatttt tttcacatct tcttgcggga tttcaccaag ttctgcccaa gcttcatctg
     3781 ctaaaatctc gacttcgagc cacgcacggt atttgttctc ttccgtccag atattcccca
     3841 tttcagggcg tgtataacgt tctaacatgt tttttctcct ctaatcccaa atattcgttt
     3901 gtttgatttc ttccagcgtt tgctggatgt cttctgtcaa gatagtgata tgtcccatct
     3961 tgcgaccttt ttttgcttgt ttttttccat agaaatggaa ttgccagtct ggcttattct
     4021 caatcaagcg atagctttcc agcagttgtt ctcccaaaac attgaccatt actgcattgc
     4081 ttatcaaacg tactttttct cctaatggcc aaccacaaat acctcgaata tgggcatcga
     4141 actgactcat agaacaagct tcaatagaat aatgtccaga attgtgagga cgaggagcta
     4201 attcattcac atatagcccg cctgtttttg ttaaaaacat ttctacccct aaaacacctg
     4261 aaaggctcac tgcttctgca atcacacggg caatacgctc agcctcctcg atcacatcct
     4321 gatcaatcgc ggctggagca atcgtttcat gaagaatatt gttgtggtgg atgttttcca
     4381 cgaccggaaa agtcgtgaaa tctccttgac catttcctgc caccatgatc gaaatctctt
     4441 tttcaaacgg gatccatgct tctaaaacac aggttccttc tcttaaaagg tccatggcag
     4501 gagctagatc cgcccgactt ttcaaaacat actgcccctt gccatcatag ccgcctcgag
     4561 tggttttcaa aacacaagga tagccgatcc cgtcaatcgc atcttggata tccgtcggac
     4621 tgacgatcgt tgcataagga gcaatcacga tgttattcgt ttccaaaaaa gatttttcta
     4681 acaggcggtc ttgcgtaatc gcaagcaagt ctgtcccttg aggaataaat gacatgggta
     4741 aaatcgcatt taacgcctct acactgacgt tttcgaattc ataggtaatg acatctgttc
     4801 ttctggcaag ctctttcagc gcaaaggtgt catcatagac actttcaatg tgccaatctg
     4861 caacttgtgc ggctggacaa tcgctcacag gatccagcac acctacttta aagcccattt
     4921 cttttgcact aatcgccatc atacgaccta attgaccgcc accgacgatc ccgatcgttg
     4981 aaccaggcaa caaaggttta accaagttga tcactacttt ctataacagt tttagccaac
     5041 attgaacgtc tttcagccaa tttagcctcc aattctaaat catacatgga aagcatctgt
     5101 atcgcaagta gtccagcatt aattgctccc gcttttccga ttgctgtagt agctacaggt
     5161 actcctccag gcatttggac aatcgaaaga agcgaatcca aaccatttaa tgttcgtgat
     5221 tggaccggta caccgataac cggcaaagtt gtttttgatg cgaccatacc cggcagatgc
     5281 gcggctcccc ctgctcctgc aataatgatt ttgattcctt tttttcgagc gttttgtgca
     5341 tattcaaaca tcaaatctgg ggttcgatga gcagaaacga cctttttttc ataagacaca
     5401 ccgaattcat ccaatatttg acatgcttct ttcattgttt cccaatctga agtactcccc
     5461 ataatcacag agatttttgc tgtcattgtt tttcctccca gtttttctac attcatattt
     5521 taacaatcac tttttttgat gtcaaagaaa tttcgaatgt taacaaagaa aaacatttta
     5581 ttgttcgtgt ttattaaaga taaaaaaaag gaatgtgaca aaagttaggt cacattcctt
     5641 tttatggata aacggtgttc tcctgccaac tcctcggtca caaggaacaa tattcaacaa
     5701 acatgagaag cttttttttg aaattgttcc ttgtgactcg gagatgaacg gcaggctcac
     5761 gacctcttcc ttcaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc
     5821 aaaatacgag gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt
     5881 cacaaccttt cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt
     5941 tttcaatcca ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc
     6001 atttccagtg aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt
     6061 gaaacccagc ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa
     6121 atcaacttcc aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat
     6181 tcctccacca agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa
     6241 tcctaaaaca atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga
     6301 aagttgaacc aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc
     6361 gcctaagata acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac
     6421 atttttccca gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac
     6481 catggaaatg atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata
     6541 aaagggttgt ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc
     6601 catgaaagca gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat
     6661 gaaccctttt gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag
     6721 cagatttgtt ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat
     6781 tgctacagga atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg
     6841 gaatagtttt ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc
     6901 gataatagat ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc
     6961 tactgcttgg atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac
     7021 tgtcaattgc aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt
     7081 catctgttct tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc
     7141 tgcatacatc gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc
     7201 tttttccacg attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca
     7261 atacgcgcaa gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg
     7321 aaagactttt cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat
     7381 tcgatcaatc cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg
     7441 tcttctgaag ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa
     7501 gaataaacag aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg
     7561 aagatcattg gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct
     7621 tcaatggtca caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc
     7681 atttgttcca tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg
     7741 ccctcgccta atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt
     7801 gctcctttat catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt
     7861 ctgttaaaga aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct
     7921 tttacttata gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat
     7981 atataagtag gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc
     8041 acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc
     8101 taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat
     8161 ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct
     8221 aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc
     8281 gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg
     8341 gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac
     8401 tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc
     8461 atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc
     8521 atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc
     8581 atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc
     8641 ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc
     8701 tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata
     8761 cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat
     8821 atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata
     8881 catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag
     8941 ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat
     9001 cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa
     9061 attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt
     9121 atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa
     9181 gtggatagaa aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg
     9241 atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac
     9301 cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc
     9361 atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat
     9421 aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt
     9481 ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt
     9541 ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc
     9601 ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta
     9661 aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt
     9721 aatgcagtga gtaaaaaatc atgctgtata caccagaaca gtccatacaa aaggcataaa
     9781 taccacagaa accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa
     9841 acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg
     9901 gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc
     9961 actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg
    10021 aaacacatcc gtttgatttt tcctttcctc caacatacct ttttttctgc caagtaatgg
    10081 acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca
    10141 gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg
    10201 ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct
    10261 tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat
    10321 atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa
    10381 acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata
    10441 ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc
    10501 aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt
    10561 tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg
    10621 ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg
    10681 gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg
    10741 gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc
    10801 tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg
    10861 tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa
    10921 ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca
    10981 aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata
    11041 agaccggata aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg
    11101 ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat
    11161 ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta
    11221 gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc
    11281 gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc
    11341 gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc
    11401 ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct
    11461 gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac
    11521 atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg
    11581 cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact
    11641 agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa
    11701 gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc
    11761 acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca
    11821 tcactcagca tctttatata attatccaag ccgatccatg agactggatt tcccaaacga
    11881 taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat
    11941 cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt
    12001 tttagttttt cttttttcat gccttcctcc tgctgctatc ctagaatgtt tgacgcaaac
    12061 tcataaagac tctctgctgc ttcttctgct gttacttctt cataaatgac tttttcaact
    12121 aaaagattgt attggtcttg aatttccgga ttcaaagcac tatcaaagac attcacttcg
    12181 ccgttgtcca tatcggaaat catttctaaa taatcaaatg tcgcttgcaa agttgcatca
    12241 tctgcacgag cgagctgttc tcgaatattc gacatgattg gtacaccacg ttctcctttt
    12301 aaaatttcat tggcttcttc actgttttga aagaaatcaa taaacttagc agcttcttct
    12361 ttatgggctg agctttttgc catactcagc atttgcgaag aacgtaaagg gataccagat
    12421 tctccatctg cttttctgcg aggtggatta atcaactgaa tctccatatc ttcaggagcc
    12481 cctgacaata tttctgtcaa ctggttactt gatagaaaca ccattgccgc ttctctatcc
    12541 ctaaccgcct gaaaagacag atccgtcgta cttgcagctc caccacctgg ttctggatgg
    12601 gcccctttgt ctaccagatt tttacgcagc tggaaaaaat ctgttagata gtctggattt
    12661 tcaaatccca agctgttaga tagatcttcc cgattataaa aattcaatcc tttctcaact
    12721 tgtggtattt gaataataca gccagctata aaatcttcga atcttgaaat agcatagatt
    12781 cctaattctt cagtgatttg atagctgatt tgttcaaatt catcccatgt ccaatttgaa
    12841 gctggttcag ggatatttaa ttgttcaaat atatttttat tgtaagcaat cgcaaacgaa
    12901 ttcaccccgt tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc taaataggat
    12961 tcatctgcat tagaaatatc aattatccct tgctccacat attcatctaa tggttcaatc
    13021 tgattgatgt attgaggaaa ttgattgttc agttggaaaa tgtcccagat atcatctgat
    13081 gctgctagtg tacctaattt tgtgtaatat ccttcataat cataaaattc ataatcgatc
    13141 gtcacatggg gatttttttc ttcataaagc tcgattactt ttactgttgc atcgtgtcgg
    13201 acttgcgaac cccaccaagc catgttcagc gtgatttttt tgtcatcttc ttgcccacaa
    13261 gccgtcaaga agaataacat aacacttaaa gctaagatga ttctcattct tttcttcatt
    13321 ctctgcacct ctacttccat tgttattttt caatatactg tatttttata cagaacatat
    13381 ttattaaacg atctttttca aggggaataa aaaccaagac ttgccttatc gttctgccta
    13441 ggatgaacaa ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc tttcatcgct
    13501 aaaaacactt cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa ggatatcaga
    13561 caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg cgctcttccc
    13621 taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat acacgtcgat
    13681 gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt caagccccga
    13741 caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt ttttcattct
    13801 atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata ataacaaaag
    13861 gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct cttttttttg
    13921 aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc
    13981 tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc gatatttttc
    14041 cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc
    14101 tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag
    14161 ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat
    14221 attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac
    14281 cgtactgata actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt
    14341 aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga
    14401 agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc
    14461 ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc
    14521 attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa
    14581 ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc
    14641 ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt
    14701 tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc
    14761 gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag
    14821 ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac
    14881 agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg
    14941 atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc
    15001 ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa
    15061 tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt
    15121 agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt
    15181 tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag
    15241 agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt
    15301 ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct
    15361 tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag
    15421 tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata
    15481 aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt
    15541 gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc
    15601 agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc
    15661 gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact
    15721 tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct
    15781 tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg
    15841 ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga
    15901 ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga
    15961 tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta
    16021 gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca
    16081 tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct
    16141 aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg
    16201 aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc
    16261 gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc
    16321 atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa
    16381 agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt
    16441 gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg
    16501 caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat
    16561 cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat
    16621 cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat
    16681 aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag
    16741 ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct
    16801 ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa
    16861 ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca
    16921 tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt
    16981 ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg
    17041 gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa
    17101 atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg
    17161 aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa
    17221 agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac
    17281 gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca
    17341 gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc
    17401 tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg
    17461 aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta
    17521 tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc
    17581 caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg
    17641 ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata
    17701 catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc
    17761 ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc
    17821 tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag
    17881 aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag
    17941 gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca
    18001 caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga
    18061 ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa
    18121 tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat
    18181 ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc
    18241 agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt
    18301 taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc
    18361 atagatatgt tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc
    18421 tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg
    18481 tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt
    18541 cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc
    18601 catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg
    18661 cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat
    18721 taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt
    18781 gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat
    18841 gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa
    18901 cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt
    18961 agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa
    19021 cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt
    19081 tcgcattcct atacgcattt acttccccct tttttatttt aattatactt gaataatgaa
    19141 aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc
    19201 tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt
    19261 tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc
    19321 tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat
    19381 catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt
    19441 tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt
    19501 cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct
    19561 gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga
    19621 tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac
    19681 agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct
    19741 aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga
    19801 ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc
    19861 catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg
    19921 cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct
    19981 ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa
    20041 gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc
    20101 ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca
    20161 attgcggtag catatcgata accatactca agacagctgc ttcgccatat tccttgaagg
    20221 cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg
    20281 cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac
    20341 ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt
    20401 ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac
    20461 gacgtgtgat ttctttttct tccagttcga tttgtttttg acgttctacg attttaatct
    20521 gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt
    20581 ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt
    20641 cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt
    20701 ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat
    20761 ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc
    20821 tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag
    20881 cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca
    20941 tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt
    21001 cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac
    21061 cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt
    21121 caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa
    21181 ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa
    21241 tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg
    21301 ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg
    21361 cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca
    21421 tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg
    21481 ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac
    21541 cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa
    21601 cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca
    21661 tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag
    21721 caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc
    21781 ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata
    21841 catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat
    21901 aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta
    21961 tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat
    22021 aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc
    22081 acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct
    22141 ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc
    22201 catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc
    22261 tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga
    22321 cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt
    22381 tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg
    22441 tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc
    22501 tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca
    22561 agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa
    22621 agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg
    22681 cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat
    22741 agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc
    22801 catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca
    22861 aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt
    22921 ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat
    22981 gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca
    23041 gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg
    23101 aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc
    23161 atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta
    23221 aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt
    23281 gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta
    23341 gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa
    23401 gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac
    23461 ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc
    23521 tgcgatgacg gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt
    23581 tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc
    23641 cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa
    23701 ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat
    23761 ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag
    23821 tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct
    23881 gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca
    23941 aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa
    24001 tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca
    24061 gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagccga
    24121 tcagaaattg atcgatcaag tattcaacca caccactccc attcacgaaa taccatcatt
    24181 catcgatttg cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg catttattct
    24241 aaaagaagat actatttttt caacaaaaga actggctcaa aaaagctcac tgtcacgtat
    24301 atccgtcaag aaatatttga attttttagt agaacaagat atattgaacg aaagaatcaa
    24361 atatttggaa atcggccggc cgctaacctg ttatgaattg acaaagaatg ggcggaaaaa
    24421 gctcacagat tactgttgag tcatcgaatt atcccataca aaatagtcca agtatacaga
    24481 agtaacaact gtttacttgg actattttta aacattttat tgacgaatat ccatgttttc
    24541 ttatcttaat tgacgggctt taggaagaga tacctcattt ttcgtttttc cagtttctat
    24601 tatttcacga aagttctcga aacttgtcga aatcatattt gataatgcct catctgtatt
    24661 tgaacctaca tgcggggtta caagaactct agggtacatt tctactaatt gctggatagc
    24721 agggtctgga atcgtctccc atggcttgaa ggatcggaaa aaaacctctg tttcatttgc
    24781 aaaaacatcg gttgcaaaac cagctaaatg attgcttttc aaagctctta aaatagcttc
    24841 attatcttga agctctccac gggctgtatt aatcaagatg ctaccttttt tcatttgagc
    24901 taaaaatcct tcattgatca tttgatcgtt ttcacctggg atatgaggta catgaatact
    24961 gacaatatcg cttttggcta ataactcttc aagcgagtgg aacgtcaata cttttttagc
    25021 agcttctgtc tgatgaatat cgtagccgat cactttggct cctagtccct taaacaaaac
    25081 agcttccgtc aaccctatcc tgcctgttcc tatgatccct actgtgcaat ttctgatttc
    25141 acgactaaac atttgctcat ccactcgaaa atctttgaaa gatgttcgga tcgtcgtata
    25201 cgcagtattt ctcaaaagca tcatggcaaa agtcaaagat aactctgcta tcgcatttgg
    25261 cgaatacgat ggtacacggg ctactgtcat accaaaatca gctgccgctt gtagatcgat
    25321 atgattgact cccacagtcc gtgtaaaaac ataacgaatc ccatattcct gcatttttgc
    25381 aagattggtc tcatctgcta tacaatttcc cctcaacaat acagcatcat ggtcctttgc
    25441 tgacacgata ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat aatggtaaat
    25501 attcaatttt tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg cgattctcat
    25561 actttcttcc tccatttctt atgaaaataa acctatgcca tgaatcattt gtaatacaac
    25621 gatccataca ggtgtcagta ccacagcaaa aatagttgac agtaaggatg cattggaggc
    25681 taaaagtgcc tccctatcaa aactgatagc ataggctgct gccactgttg cagctggtgt
    25741 agccatcatg ataacgattg ttgccagtgc ttcaaaattg actggtagaa tattcgttgc
    25801 cgtcaaaact gctagaatca cgatattcaa tgcaggtaca aataccactt tgactagact
    25861 gtaataccat gaagtttttt cagatgcagc ttttttgaag cttacttcac ctaatgttgc
    25921 gccgattgct agccacgcca atggtgaact taaaccagct aaataatcca tcggctgaaa
    25981 tagccacggt gcagtctggt caattcgcaa aaacgctact tggcttatct ctccttctgc
    26041 acctgtaact gacacttgtg gcagataccc ttggaacagc caaatgaaaa ggccggcaaa
    26101 agtagcgata atgattggat tgacgaacat cgctttgata tttttagctt ccatcttcaa
    26161 gccactcatt tttatgtatc cataagaata caaaaagatt cggtagccaa tattgaaaat
    26221 tgaggaatac atcacgccga caggaccata aatcgcactg acgataggcg ttccgaaaaa
    26281 ggtcgtagaa ccaaagatcg tcaggacacg caacgttgtt tgcttatccc ctttatagct
    26341 taagaaaaaa ggttttgaaa ctagaataag aataatataa ataactaatc cccaaatcaa
    26401 gacattgatt ccttgcatca gtaattctgt attgatatct tgcatgaaag ctcgaaatgc
    26461 cagtgctggc aaagccactg ttaagacgac tttcgataga atttttccaa cagattcaga
    26521 aaaaatcccc ttttttctac aaacaaaccc taataatata ataaaaactg ttgaagtgat
    26581 tgcactgaca atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata agttcatctt
    26641 tctcctccta attatgttca tttattcaca ataaaaaaag cttttatttt gctaatacgt
    26701 attaacattt ttaattttat ctttacattg ttacttattg aacaatagga aattaaagaa
    26761 acttttgaaa cttatgtaaa gtgattttt
//
LOCUS       DAZKAO010000027        25502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000027 DAZKAO010000000
VERSION     DAZKAO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.027"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8H8_000921"
     CDS             133..3660
                     /locus_tag="ACG8H8_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP8986011.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8H8_000922"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8H8_000922"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP8986012.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8H8_000923"
     CDS             7448..7789
                     /locus_tag="ACG8H8_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP8986013.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8H8_000924"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8H8_000924"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP8986014.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8H8_000925"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8H8_000925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP8986015.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8H8_000926"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8H8_000926"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP8986016.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8H8_000927"
     CDS             13000..13737
                     /locus_tag="ACG8H8_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8986017.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8H8_000928"
     CDS             13751..14566
                     /locus_tag="ACG8H8_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8986018.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8H8_000929"
     CDS             14579..15226
                     /locus_tag="ACG8H8_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8986019.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8H8_000930"
     CDS             15242..15898
                     /locus_tag="ACG8H8_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8986020.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8H8_000931"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8H8_000931"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP8986021.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8H8_000932"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8H8_000932"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP8986022.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8H8_000933"
     CDS             18240..18758
                     /locus_tag="ACG8H8_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8986023.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8H8_000934"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8H8_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP8986024.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8H8_000935"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8H8_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP8986025.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8H8_000936"
     CDS             20691..21743
                     /locus_tag="ACG8H8_000936"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP8986026.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8H8_000937"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8H8_000937"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP8986027.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8H8_000938"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8H8_000938"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP8986028.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8H8_000939"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8H8_000939"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8986029.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8H8_000940"
     CDS             24333..25427
                     /locus_tag="ACG8H8_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP8986030.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAZKAO010000028        25417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000028 DAZKAO010000000
VERSION     DAZKAO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.028"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8H8_000941"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8H8_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP8986031.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8H8_000942"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8H8_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP8986032.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8H8_000943"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8H8_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP8986033.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8H8_000944"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8H8_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986034.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8H8_000945"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8H8_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP8986035.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8H8_000946"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8H8_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP8986036.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8H8_000947"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8H8_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986037.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8H8_000948"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8H8_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP8986038.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG8H8_000949"
     CDS             complement(7139..7417)
                     /locus_tag="ACG8H8_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP8986039.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG8H8_000950"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8H8_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP8986040.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8H8_000951"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8H8_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP8986041.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8H8_000952"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8H8_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8986042.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8H8_000953"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8H8_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP8986043.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSVLESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8H8_000954"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8H8_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP8986044.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8H8_000955"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8H8_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP8986045.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8H8_000956"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8H8_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFP8986046.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8H8_000957"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8H8_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986047.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8H8_000958"
     CDS             13967..14695
                     /locus_tag="ACG8H8_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFP8986048.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8H8_000959"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8H8_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP8986049.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8H8_000960"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8H8_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986050.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8H8_000961"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8H8_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986051.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8H8_000962"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8H8_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFP8986052.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8H8_000963"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8H8_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986053.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8H8_000964"
     CDS             16394..16606
                     /locus_tag="ACG8H8_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986054.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8H8_000965"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8H8_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986055.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8H8_000966"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8H8_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986056.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8H8_000967"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8H8_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986057.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8H8_000968"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8H8_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986058.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8H8_000969"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8H8_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP8986059.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8H8_000970"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8H8_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986060.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8H8_000971"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8H8_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986061.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8H8_000972"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8H8_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986062.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8H8_000973"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8H8_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986063.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG8H8_000974"
     CDS             complement(19851..20543)
                     /locus_tag="ACG8H8_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP8986064.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG8H8_000975"
     CDS             complement(20549..21220)
                     /locus_tag="ACG8H8_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP8986065.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG8H8_000976"
     CDS             complement(21213..21554)
                     /locus_tag="ACG8H8_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986066.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG8H8_000977"
     CDS             complement(21732..22430)
                     /locus_tag="ACG8H8_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP8986067.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACG8H8_000978"
     CDS             22517..22987
                     /locus_tag="ACG8H8_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986068.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACG8H8_000979"
     CDS             complement(23030..23209)
                     /locus_tag="ACG8H8_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986069.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACG8H8_000980"
     CDS             complement(23196..23534)
                     /locus_tag="ACG8H8_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP8986070.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="ACG8H8_000981"
     CDS             complement(23550..23753)
                     /locus_tag="ACG8H8_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986071.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="ACG8H8_000982"
     CDS             complement(23768..24544)
                     /locus_tag="ACG8H8_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP8986072.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="ACG8H8_000983"
     CDS             24843..25163
                     /locus_tag="ACG8H8_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986073.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..>25417
                     /locus_tag="ACG8H8_000984"
     CDS             25181..>25417
                     /locus_tag="ACG8H8_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP8986074.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFE"
BASE COUNT         7102 a         5268 c         3942 g         9105 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaaacag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaa
//
LOCUS       DAZKAO010000029        25091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000029 DAZKAO010000000
VERSION     DAZKAO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.029"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..1341
                     /locus_tag="ACG8H8_000985"
     CDS             196..1341
                     /locus_tag="ACG8H8_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8986075.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1426..1731)
                     /locus_tag="ACG8H8_000986"
     CDS             complement(1426..1731)
                     /locus_tag="ACG8H8_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP8986076.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1756..2187)
                     /gene="ruvX"
                     /locus_tag="ACG8H8_000987"
     CDS             complement(1756..2187)
                     /gene="ruvX"
                     /locus_tag="ACG8H8_000987"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP8986077.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2184..2450)
                     /locus_tag="ACG8H8_000988"
     CDS             complement(2184..2450)
                     /locus_tag="ACG8H8_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP8986078.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2705..3430)
                     /locus_tag="ACG8H8_000989"
     CDS             complement(2705..3430)
                     /locus_tag="ACG8H8_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP8986079.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3791..3861)
                     /locus_tag="ACG8H8_000990"
     tRNA            complement(3791..3861)
                     /locus_tag="ACG8H8_000990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3827..3829),aa:Gly,seq:ccc)
     gene            3978..4730
                     /locus_tag="ACG8H8_000991"
     CDS             3978..4730
                     /locus_tag="ACG8H8_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP8986080.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4746..5225
                     /locus_tag="ACG8H8_000992"
     CDS             4746..5225
                     /locus_tag="ACG8H8_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP8986081.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5476..7056)
                     /locus_tag="ACG8H8_000993"
     CDS             complement(5476..7056)
                     /locus_tag="ACG8H8_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP8986082.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7049..7792)
                     /locus_tag="ACG8H8_000994"
     CDS             complement(7049..7792)
                     /locus_tag="ACG8H8_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986083.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(7968..8744)
                     /locus_tag="ACG8H8_000995"
     CDS             complement(7968..8744)
                     /locus_tag="ACG8H8_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8986084.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(8992..9828)
                     /locus_tag="ACG8H8_000996"
     CDS             complement(8992..9828)
                     /locus_tag="ACG8H8_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP8986085.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(9839..10768)
                     /locus_tag="ACG8H8_000997"
     CDS             complement(9839..10768)
                     /locus_tag="ACG8H8_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP8986086.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            10960..11313
                     /locus_tag="ACG8H8_000998"
     CDS             10960..11313
                     /locus_tag="ACG8H8_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP8986087.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(11387..12043)
                     /locus_tag="ACG8H8_000999"
     CDS             complement(11387..12043)
                     /locus_tag="ACG8H8_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP8986088.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            12269..14203
                     /locus_tag="ACG8H8_001000"
     CDS             12269..14203
                     /locus_tag="ACG8H8_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8986089.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(14289..15512)
                     /locus_tag="ACG8H8_001001"
     CDS             complement(14289..15512)
                     /locus_tag="ACG8H8_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8986090.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(15506..17377)
                     /locus_tag="ACG8H8_001002"
     CDS             complement(15506..17377)
                     /locus_tag="ACG8H8_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP8986091.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(17398..18516)
                     /locus_tag="ACG8H8_001003"
     CDS             complement(17398..18516)
                     /locus_tag="ACG8H8_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8986092.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(18599..19432)
                     /locus_tag="ACG8H8_001004"
     CDS             complement(18599..19432)
                     /locus_tag="ACG8H8_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986093.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(19425..20342)
                     /locus_tag="ACG8H8_001005"
     CDS             complement(19425..20342)
                     /locus_tag="ACG8H8_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986094.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(20569..21693)
                     /locus_tag="ACG8H8_001006"
     CDS             complement(20569..21693)
                     /locus_tag="ACG8H8_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8986095.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            21876..22784
                     /locus_tag="ACG8H8_001007"
     CDS             21876..22784
                     /locus_tag="ACG8H8_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986096.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            22925..24538
                     /locus_tag="ACG8H8_001008"
     CDS             22925..24538
                     /locus_tag="ACG8H8_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8986097.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7952 a         4878 c         4177 g         8084 t
ORIGIN      
        1 cgaaatcaaa tctacaaacg caggtaacga tgaagattta ttaaattatt taaaagaatt
       61 aacatctttt attgatgaaa taaaaacaac tacattcaac aaaaaaaaat attttcaaaa
      121 tcaatatgat atagaagaat aaagattaca ttttcctatc aaaagcacgc cacgcaactg
      181 ataggaggaa aaacaatggc aacaattaaa caatatgaaa agaaagacgg aaccaaagct
      241 tggatgtttc aaacatacct tggaatcagc ccgctaactg gtaaagaagt ccgtactaca
      301 cgtagaaatt ttaaaacaaa aaaagaagct caaatagagc ttaatagact acttgtagac
      361 tttgaagaga atggacttaa aagggaaacg aacgaaactt tccaagaagt gtatgatctc
      421 tggtatgaaa gctataaaac tactgtaaaa gaagtcactc aattaaaaat tgagatcaag
      481 tttaataaat ggattcttcc aaaatatggg cagtttagaa ttaaagaaat tactacaaaa
      541 catgttcaga agatattgaa tcagtgggct aaaacaacag atcaatataa agtcctacat
      601 tctaccgtaa gccgtatttt caaatacgca atgactctag ggctgatcac taaaaatccc
      661 tgtgagctcg taatcatgcc caacagaaat gttgaaaaaa agaaggatac tgtcaaaatt
      721 tattcacgag atcaattaga aaccttattt caatatttgg aatccaaaag aagtacgtat
      781 agggcagagt acgataaaac tttactgcgc tttctatttt ttagtggctg tcgaatcagt
      841 gaggctttag ccttagactg gtcagatatt gactttgatt caaacaccgt cactataaac
      901 aaaaccttaa gccaaacaaa atatgggtac aaaatttcaa gtccaaaaac gagaaatagc
      961 tatggttccc tatctttaga caataaaacc attaattggc taaagaaatg gcaaatcaat
     1021 caaaaaagat atatgttaac cctcgggatt actgatccaa caatgatttt ttgtggaatc
     1081 tacaaagaaa tcattactaa acacgctatc tattctcgta tgatcacaat ctgcaagaat
     1141 tctggtattc catttctagg taaccatgta accagacaca ctcatgctag tatgctgtta
     1201 gagtcgggag tctccatgaa ggaagttcaa gaacgcctta gacactcatc aatcaaatta
     1261 acaatggata cctatgctca tctaactaaa gaaaccaaag aaaaaaccgt tgaaaagctt
     1321 gcagagcacc taaatttcta gcggaacgta ttcaatcccg tattcaattc aaaacaaaaa
     1381 aaagctgaac ccttatctac caagggttca gctatcattt acagcttatt cttcttcttc
     1441 cgccatgaat gtattgaata cttcttcaat catgtcccat tctgcgtctg tttcgatttg
     1501 ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt gtaattctac
     1561 atcttcatct tctgaaacac cagcaggata taaaagaaca tagttacgtc caaattcttc
     1621 ttgtccgtca actgttagta ggatttcata taatgtttca tttccttggt catccaccaa
     1681 tgtgatgtgt tcgtgtcctt catggtcgtg ggaatggtta tgttctgtca taagattacc
     1741 tcttttcttt tattattaaa ataattgctt acctgagccg tctaaataat tttgtaagat
     1801 catcacagct gctaatttat cgatgacttt ttttcgtttg gctcttgaag tatttgcctg
     1861 ctctactagc atacgttctg cctgaacagt ggttaatcgt tcatcttgat aatcaactgg
     1921 caaggagaat agttctttga tcttttctcc gtatgacatg gaagcttctg cccttggtcc
     1981 gatggaattg ttcatatttt taggtaatcc aactacaaac cggtctactt catattgtgc
     2041 tactaattcc ttcaaccgat caaagccaaa ctcttcttct gcttcattga tccgaatgat
     2101 ctctactcct tgagctgtcc agccaaaggg gtcactgata gcaacaccta ctgtcttaga
     2161 accgacatcc aagcccatta ctctcataaa ttcactccat gatttgagag gtagtccttg
     2221 acaattactt ccatgatctc atcgcgttcg tgacggcgaa tcaaatttcg agcatcacga
     2281 taacgaggaa tgtaggccgg gtctccagaa agcaagtagc cgacaatctg attgattgga
     2341 ttgtagcctt tttcttccaa agcccgataa acaatcgcta atgtttcgct cacttctttt
     2401 ttgcgactat cgtcaaaacc aaaacgtacg gtttcatctg taaaacccat gattctacac
     2461 ctctttcccg caaggtaatt ccagcttacc ttctacaacc ttattttact cgaagaaagg
     2521 gaaaactaca aacaaatatt ccaaacttcc gaataaatta agaaacacta tttgtatgtt
     2581 tttttccttt tttctgtagc tttatcaaaa gtactctttt cataaagtat acgctatatt
     2641 tctttgaatg tatgttttta cacaattttt tcataatatt cataaaaaat tcgaatagct
     2701 gctactaatt ttttgttttt gttgcttgtc ctgtctcata attgatcgtg tagccatctt
     2761 gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt tctgcttcca
     2821 attgcactcc cctggctacc agttcattcc cctcaaaatg aggagtgaca cggtagcgta
     2881 catgatgatt tgtttctttg atatatgccg caatatcatt ttcgtgagca agcatgtatg
     2941 gagcattcaa cgaacgtgtc cctgtcatca agtttttaat gttgttattt tgaccagtca
     3001 gctggaaacc aattaaatgg ctgcgattat ataaccattg cccgtcactt aatttctttt
     3061 gtttccaacc tgtcgggtca atatacaaag gctctctctt ttcagtaggg aacaaatctt
     3121 ttccaagcat agcattggct tgtcctactc tgttcaattt atcaatattt gaatagcttt
     3181 cccatgttcc tttggaaagg ctaagatcct cttttgtaaa ttccggctga ttcccattga
     3241 tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga gaagcttgtg
     3301 gtgtggaatc aaaaatagtt gtaatctgct gaaagatatc tgaatctttt tgtgtggtgg
     3361 aaacaccgaa tgcccctgca atcacagcag cgacgatccc cacgatcgcc ttggttgttt
     3421 ttttgctcat ttctttactc ctttactaac agctccctaa atcttaacat atgttcccct
     3481 ataaataaat actagaaaga aatagaaaca gcaaaaaata aaacctgttt taagatggct
     3541 taaaaataca aaaaagagac ggattcctcg tctctaatca gtcaccactc tatctgtaag
     3601 gtatttatta ttattcaaaa attttcttca tactcaagac aatacactga tttcacaacc
     3661 gcttggtaaa cggtgcgcaa aagcttgcat ctgcggtaat aagtccatcc aagttaaaaa
     3721 cataagaagt tgtttttgct tggctgttct tattacttgt tgcagagcag ctttttcaca
     3781 acctatttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga agctgggatt
     3841 ttaccactaa actacacccg caatacaaaa acagtatacg cctcttttat tccttcctca
     3901 agttttttta ttctttacgc ttctggtatg ttttgtagta aaataaaact aatcaatcaa
     3961 ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac acttgtttac
     4021 tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa gatacgatga
     4081 gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact caaaccggtc
     4141 tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc aaccgttcga
     4201 ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt gcttcagagc
     4261 taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt tctttctttc
     4321 ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg gtgttatttg
     4381 gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga tacgcagctt
     4441 atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta tcttctttat
     4501 tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat caggatctaa
     4561 tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac gctatccgtg
     4621 atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg atgatgactg
     4681 ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag gaaaggaaca
     4741 caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact gcggcatttg
     4801 ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct ggtgcttttg
     4861 gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt ggttcattgc
     4921 ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg aaaatgcccg
     4981 taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta gcttatcaag
     5041 cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg gtccaagcaa
     5101 ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt aaccataaca
     5161 tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa cggcttttta
     5221 aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt gagcgatgac
     5281 aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt aattatgagt
     5341 attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg acttaatact
     5401 gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca tgatttttgt
     5461 cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat aaacgatcca
     5521 tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc aaaatggata
     5581 aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca atgaacccat
     5641 catagttgcg actaaaccca aacttcctga acctctgtta aataattggt taatattggt
     5701 ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca ataagtagca
     5761 gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct gaaaagacag
     5821 tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc cgaatctgaa
     5881 cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca gaaaattttc
     5941 ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta agaaagccaa
     6001 tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag ttaattttat
     6061 ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg aagaagaaag
     6121 aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa ataaaagctg
     6181 atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt ttgaatcagt
     6241 cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa atcccagagc
     6301 cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct gactggcagc
     6361 atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat tttcaatagt
     6421 ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg agctgccaag
     6481 caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag ccaaaccaaa
     6541 aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca aaaggattga
     6601 gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa taaacatgac
     6661 tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct ttgctgaaaa
     6721 aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc cttcaaaaaa
     6781 gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa gtgccacgta
     6841 ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa atccgtaaat
     6901 cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag ttaatcgctt
     6961 tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat ataaaagatt
     7021 aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct gtcgcttgcc
     7081 ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca ttcaataatt
     7141 cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt ttatcgcata
     7201 actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct tttgcggcat
     7261 gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct tgcaaaggtt
     7321 catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc gttttttgac
     7381 gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata tcaaaaagcg
     7441 ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag gcagctgaaa
     7501 tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa tcaggcacat
     7561 aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca tctactaaaa
     7621 tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt gattttccgg
     7681 caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga gacaagtctt
     7741 ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc atttctgtac
     7801 tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt aaatgtcaaa
     7861 aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt gttctcttct
     7921 acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta aaatgattca
     7981 ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt tcccatctct
     8041 agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga tgtaatgttt
     8101 tcagtattaa tgatataagc ttttaaatct ttacataaat actcatattc gagtgatttt
     8161 ttatattttt taattgtgcc gtttacgaga atctcttcat ctttcaatac taggcctatt
     8221 ttatttttat aacctggcat gctcattaga taaaggattt catcgtaatg gatatagtga
     8281 tcgctattac cataacggac agttgtccac ctttttttcg tagcatgttg aacaggatag
     8341 ttttcttttt ctttcaataa gtaactttgg atgctttttt tatttttttc ataagtgaat
     8401 tcttttatca aataaccact aggcgtaatc tttcgattaa taacagttat tgctttattc
     8461 ggatgagacg ttagataaac aattttacac ttttcattct tttctctaag catttcgcct
     8521 agttcgatac cgctataata ggcgttcaaa tcgatgtcta ggaaaaaaat atctgaggaa
     8581 tagatttcat tatttttaat gaattcaaca aattgtataa cgttcaatcc ctgaacgatc
     8641 tcaaatttcc aaggacttaa taaaattctg gattcttcca aaatttctac tagcattttt
     8701 tcaaaaacca aactatcatc caaaataaat atacgcgtta ccataaaaag tacctccact
     8761 cgatatttta ctatcaaaat catttcatac aacttttcaa catacatgtt aggaataaat
     8821 tgtaattctc ttcacaatct tttccaacac ctttgaaacc gatatcaaat actttcagga
     8881 tgctgcttct aaaataaata tcaaacaaat gaatacatca tgcaaaaaaa gccagacaac
     8941 cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt attttgttct tttaaaaaga
     9001 acgatacaca tactgatttt tcggagcaga gactttctca gcgtttgtaa caactacaga
     9061 tgggatttca cgcttgaatg gttgataata gtctgattgg atggttcctt ctattttata
     9121 ccattcatca tttttaaccg ttactccttc cggcatatgc gtcaacaaac caaagactcc
     9181 tgaatctgcc acacaatgga taatgccgaa tcggaacaag aagatatctg tattgctatc
     9241 gttcggtgtt tggtaaacaa atccttcata gctgattttt ttgccaatga attcgctagg
     9301 gtaattataa attagctcca taacttctaa atagttttca tctgtgatct tgatagtttg
     9361 cttatcccca tacttttctt tcaactcatt catttggtct aagtagtctg atttgttgaa
     9421 ataaatactg gtatccggct ttaagtattg tgtctgcatc tccggatccc ctactgattc
     9481 tttacttact gggaaattaa atcctttggc ttctacgatc gtagtgtcga gactaactgt
     9541 tggaaacaac gtccccacga ttactggcaa agctagcagt acataagcga ttccacgttg
     9601 ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt gccttttctt ctgcttttcc
     9661 gtttttgacc cacaaataca gctgaactaa agccaaaaga gcagataaaa tcattgaaag
     9721 aatagctaag tagcgataat ggacattgat atattgatcg agtttgccag aaacttgcaa
     9781 gtacatcatc aattcgaaat acccagataa aatcaaaaac cgcagcatat tttccctcct
     9841 aaataaataa tgtgaatcca ataacgctaa gggcaatcag tccgatcagc gataaaataa
     9901 agcgtcctgt aaaatatcgt ttcatcatca gtagattttt gatatcgacc attgggccaa
     9961 agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact taacaaagaa gatcctataa
    10021 acgcatctgc ttctgagcaa agagacatcg taaaggccaa taacagcatg atgataatgg
    10081 ctaaaatctt tgttgagccg agcgttagca taattctagt tggtaaataa gtctgcatcg
    10141 cagctgcgat taatccgcca atgatcaaat aacgacctgt atcaaaaaat tcgtcgattg
    10201 aatgtgtcaa cacatgccca gtttgatgga tcagctgatt ttttggttca ttcttctcac
    10261 tttgttcttc ttcatggaca tgatgatggt gattttctgc tactttagac gtgatttttt
    10321 ctttcaatac agcgtctttt tgaaaatagg caagccagac accaatcaca agggcaacaa
    10381 aaaagctgcc tatcacccgc cagcttgcca tctgccaaga attgccaaat gcgataaaag
    10441 tggaaaaaat cacgattgga ttgatgatcg gagcagtcag cataaaagca aacgctgtgt
    10501 gaactggtac gcctttttta acaaattgat gaacgatcgg tacgatgccg cattcacatg
    10561 aaggaaagaa aaatcctaag atcgaaccag taaaaataga gaagatacga ttttcaggaa
    10621 gccatctctt cacacgttct ggggtcaaaa atacttgtaa ggcgcctgag ataagacagc
    10681 ctaataatac gaaaggtaat gcttcaataa cgatcgataa gaaaatcgta ctcatctgca
    10741 aaaccgaatt tggaataaaa gaaaacatga gtttcctcct ttttatttct agttcattcg
    10801 ctttgcaaat atacgctttt attcaaaaaa aacaagttgc tctgtaaatt tttacacaac
    10861 tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt aaattaaggt
    10921 ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac cactaacttg
    10981 actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct ttcgatgcct
    11041 aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac acattatgat
    11101 attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa atttgaagtg
    11161 ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg gcaagcacgg
    11221 ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga agatgattcc
    11281 ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa aaaagcgatt
    11341 gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat tctgctgtat
    11401 tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc aattcattcg
    11461 tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca gcggtaaagt
    11521 tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt gctctttcag
    11581 cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct gacatatctt
    11641 ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca ttcacatcaa
    11701 aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt aaagcacttc
    11761 ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt aatgtctttg
    11821 caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct aattcgccaa
    11881 aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca cttaattcag
    11941 ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga taatacaaag
    12001 gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga cctccacgta
    12061 ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa aaatcaattc
    12121 cattgctcag atattgtgaa attttattct ttattcattt atttatcatt tctgcaaatc
    12181 tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt tatttgagga
    12241 gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc agattgctcg
    12301 ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta caggcgctcg
    12361 tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga tcattgctgg
    12421 aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc tcggatacct
    12481 cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc tgaaagcttt
    12541 tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca ttagcgaagg
    12601 tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc tgcaacacga
    12661 tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat tgcatgggtt
    12721 taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg gacagcgcac
    12781 acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc tagatgaacc
    12841 aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc aaagctatac
    12901 tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag tcaatgaagt
    12961 ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga aatatctaga
    13021 tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc aaacagaaat
    13081 ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca caaaacgagc
    13141 gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc aaggggatga
    13201 aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag tcttgcaaat
    13261 cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca atctagatat
    13321 ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat cgacgttgct
    13381 gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag ggacaaatgt
    13441 caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga cgatcctagc
    13501 agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg ctttaggtgc
    13561 tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg gcggtgaaaa
    13621 agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga ttttggatga
    13681 accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat tgatcgatta
    13741 tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga tcgcgacaaa
    13801 agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg attattattt
    13861 agagaaaaag caggaagaag aagaaatcgc cgaattgttg gcacaagatg agccagaatc
    13921 tgctcctgct cccacgaaca aatctgatta ttaccaaagc aaagaacagc aaaagcttat
    13981 ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa atggcgcgga tcgatgaatg
    14041 gatcgatcag ttggaacaag aaatggtcgc gccagaaaac ctggaagatc acgtaaaact
    14101 caatgagctg aaccaagatt tagaagctgc tagacaagat caagaaacaa aattgacgga
    14161 atgggaagaa ctaagcttgg aattagaaga atatgaaaat taacgaaaaa accaaaacag
    14221 aaaccattca tgctgttttg gtttttcttt ttttatttta tcaaacaaaa attccatatt
    14281 tacctttttc accaataaga agaccaatta ctagtcattc tcactaaagc ttctaaataa
    14341 tagtaatcac cccaaataca aggtgtattt accccattat ttttgggctt attataaaca
    14401 gactgtgtta aaaagccatc ttcttcttcc gttgtagcat atctttcttt caattgtcgc
    14461 ataattttcc aaacagcaga ctggtataag ttcttttctg ttttgtctaa tagacctgat
    14521 tctattaatt ctaataatcc acaaaccatg atcaccatac tagatgtatc tttttcttct
    14581 ctgctagaat catcaaagat caaatcccaa taacaaatgg tatcttctgg caaccgattc
    14641 ataaaataat tggctaatat tttgcttaaa tcaaaatatt tttgttcttt caaataacga
    14701 taacttaatg caaaaccgta aatcccccat gcttggcctc tagcccaaca ggaattatca
    14761 ctataacctt gatgcgtact tccatatctt ggttttccat ctttgacatc catataaaat
    14821 gtatgataag ttgaaccatc ttctcttact aaataatctg ctgcattttg aatatgagcc
    14881 tgagcagctt ttttatattt ttgatttcca gtttcttcac tcgcccaata caataatgga
    14941 agattcatgc aacaatccat gatcattcta ccttgttgtg aaggatcact aggatctccc
    15001 caagcttgta taactcctgc cttctcccaa aaacgctgaa gtaatgtttc tgcagcatca
    15061 attgaagctt ccttcatttt tcgatttccc gttattttat aatctgctac ggttgataaa
    15121 gaatacaaaa aaccaagatc gtgatgatta gtgataattt gacagtcaat cctgtggata
    15181 aaatctatat ctgctttttc agctgtttgt tgaaataaag gatcacccgt cttctcaaat
    15241 aacaaccaat taattcctgt ccaaaatcct tctgtccaac caatattgtc tcttaatgga
    15301 taacgtcctt ttataggaca ctcgcttggg aattgatact gactgttact gatacgtttg
    15361 attaacctct ccgtgtgtct gcgacaacgc tcgatctcat tattccattc tttttccatt
    15421 aattctttat aacattcatt tatccatttt tctggatgta ttatctcttc aaacatcact
    15481 ttatcaagca tttccccacc agcctttagc ataattttag tcttttttta ttgtttttat
    15541 tttggatagt tagcccggtt gtcccatcaa aatcaatttt gggacgattg ttccatactt
    15601 ctaaactata atcccgatct tgatgagcaa ccactcctgt tccgagccaa tgggttcctt
    15661 ttccaaatgt accctctaat ccaggaataa aagtgttttc ccatgtcatc aagtttgtat
    15721 tgggcaaacc cataatagct gtggattttc gtttaatagt atcgtcactt aaacagataa
    15781 tacctgaaaa gccagcttca ctcaccctat agttttcctt gtcttcttct atgattattc
    15841 cttctttctc aggagaacat tcaatagcaa accctgtctc atatagaaca tattctgtat
    15901 ctaattctac tttgtgtacg cgaatctgcc agccttcaaa aggaataagc caagttctga
    15961 tttttgcctg taaagtaggt tcccatgtag aggcaagtac ctctttctta catacaatgt
    16021 ctcgtacatt atttcgaacg atgaactgtc cataggtttt gatgctctca ttcttaaaac
    16081 tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat tgaatccatt gccatttctg
    16141 ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg actagaataa gcaaatttta
    16201 aatatttttc attggtcaat gtatggaaat ttggccctaa ttgtcccgca ttcaaaaaaa
    16261 atgtatgtcc attatcatgt tgtatgagca aattggcttc tgaaagaagt ttagttcctt
    16321 cacgaatagg cataggcatt tctttagccg tccaatactc atggtcttca ggtagcgcca
    16381 acaataaaaa aatcttattc agccataatg gagataacgt actattatag ggttcagtca
    16441 atgtcaattg agggtaggta taaccaatat tcaggatacc tttttcatca aatatttctt
    16501 tttgcatcca ccatcttata ttccgattga ctaacccctt gatttcacct aatttaaaag
    16561 gcaaaagatc ggcccacaac atagctgacc aaaagcttga aactgcaaaa cgataaatca
    16621 tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa aaaatgaata tagtctctag
    16681 aaaaatcaat agctcggttc ttatatatct ggctaatggg cttttctggt tctagtacgc
    16741 tataaatcaa accataataa tgaaatgcaa aaggattata ataatcttgg cgacctcttg
    16801 aactgtctcg ataccagcca ttttgaagat aacaattttc tatattatca aatgcttctt
    16861 tcagtgctga ttctttaact ggataaccaa atttttggat aattgttccg acaattatct
    16921 taaaaaattg ccaattacca tctgcacatt catattcaaa aatttgcgag aaccaatgaa
    16981 taatctggct tttttctcct gtatctaatt ttttccataa taattgttca tgatgatata
    17041 acgctaataa cattgcgggc atttctacca tacgctggtc tctttcatgt atttctcccc
    17101 aataatcggg atgggaagga tctgttccag cttttattcc tgatagaatt aatttcaacc
    17161 atcgttcatc taattggtca attgcgggcc ccgctcccca taaaacccga gagaatcctt
    17221 ccattcctgc aattttattt ccatatccta ccccttgatg tatataatca atttgtgcgc
    17281 cacttgctcg ataaaacggc tggagtggtt gatttatctt tttaaataag tcaaagtaat
    17341 cctgtctggt ccttaatagt ggtactggat gtttcattgt attttctcct ctttttacta
    17401 ccaaatagct aattccgact gttctaatcg aagtaagcct tcaataaaaa aataatcaga
    17461 atagatcagt ggcacattat aagtcagttc tctatggtat gcttctgctc cgcctccaat
    17521 gatcccatct tcatccatat tccaatttgc atagtgcttt tctgtctttt ggagtatatt
    17581 gatagcagct tcatgataca tttttgatcg tacagaggaa acatatcctg ctaattccag
    17641 aagaccgcat gcagcgcata ctccagcact ggtatcaata ttcgaatctt ttacagcaaa
    17701 atcaatgaga ggaatataac cagttttttc gattttctga ataaaattta ctgctatctt
    17761 ttctgctata gctagatagt acggattttt tgtgtaacga taacttaacg cgaatccata
    17821 taaagcccat gcatttcctc tgctccaaca agaattctca ttgtatcctt gcccgccaag
    17881 taaccgaata tatttccctg attctgcatc aaattctgcg atatgcccgg ttgatccatc
    17941 tttcctcact agatacttag ctgttgtatc tgcatgctgc cgagcaattt tagagaacct
    18001 cggatcacca gtttccctcg aagcccaata cagtaatggt aaattcatca tactatcaat
    18061 aatcatccaa ccaggtttat ggtcattcca tgctactaaa tagttccctg aaatattata
    18121 gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt cgactatgtt tgttttgaga
    18181 ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa cctacatcat gatgaacgtc
    18241 tgaaaaatgg tacagggcag attctaataa ttgttcttgt tgtattgctg cctctttgaa
    18301 cttttctcgt tttgtcttgt gaaagagctg ccataaaata ccactccaaa aaccattggt
    18361 ccaccagtcg attcctctgt cctttattac gtctgtatat tttccgttgt aagaaatata
    18421 tggaatttta ttccccattc tgtctaattc cgcatctatc ttttcagaag ctacattcaa
    18481 aatttttctt gaccattcct tcgtctgttc attcatggat atcccgccat tcttttaatt
    18541 tttatgaatg tggagttgct cctagaacga actagatggt taacattcgt tatttttttt
    18601 acttatttgc agctccaatg tcattctcct taaatttaga ctgcgcccac acataaatca
    18661 agaccattgg gatcactgaa gttgctaaaa ttagcataat tgcgccataa ttaattggct
    18721 cacctgtact agaaaccgtc atatttttga taattaaggg aaccgtatat ttatctttat
    18781 ctgaaagcat gactaatgga aatagatatt gattccaact tcccataaag ataagtaaac
    18841 cactagtaat aatcgtaggt gataaaatcg gtacagcgat tttcgtaaaa atcacccact
    18901 cattggcacc atcgattctg gcagcctcca tcagtgctgt aggaaattga ctgctatatt
    18961 gtcgcatcat aaacactgaa gttgtactgg caatcgaagg gatgatcaca cctgcataag
    19021 tattcgataa tcctaaacga ctcataatgt aaaataaagg aatcaatgtg gtaaaaaaag
    19081 gcaccatcct agatagcata actgtattga acaacagttt attccccata aaacgatact
    19141 ttgctaatgc ataacccgcc attgtagaac tggctgtagc taaaactgta ctgattacag
    19201 caataacaat cgaattccaa ataatcaagg gataattata tctcgccatg atggcttgcc
    19261 agttttttaa tagcatatcc ccaaacttga aattgattcc gccacttaaa atttcttgat
    19321 tactttgcgt tgaagctaca atcatatata aatatggcat caaaaatatt aaggcaccaa
    19381 acacaataat gacgtacgta aaaattctgc tttgtatctt ttgtttagtc atttttatca
    19441 cctccgaatt taaattgaat agccgataat ataacagata caaacaccat cgtccaggca
    19501 atagcagagg cttttccaaa atcaaccatt tcaaaacttg ttttatataa attgactcct
    19561 acgaccaaag ctgaattttc tggtccattg gaagtcccaa agaatatatt tgggatattg
    19621 aaattaccca agccattgat agtggctaaa actgtggcca ttaaaatgat tggctttagc
    19681 atggggatcg ttaataaaaa gaattgccga aatttcgatg ctccatctat ttcagcactt
    19741 tcgtatatct ctttagaaat cccttgaaga ccagctaaaa ataaaatagt gttatatcct
    19801 acactaagcc aaatcatcgt gatgatcatt gatattcgta tccaaaaagg atttgttaac
    19861 cactgtacac cgttaattcc tagcgcattc aaaaatccat tgaataaccc agtcggattg
    19921 aaaaaagtca tgaatacgga agctacagcc accgttgatg taatggttgg aatataatag
    19981 acagttctaa aaaatccttt tccttttaac tgtgctgaat tcaataaaac agcaaagtaa
    20041 atagctaata gcatcgttat tggaattgat cctgccatga taatcaaaac attcgtgtaa
    20101 gatttaataa acaatggatc tcggaagata gctagataat tgtcaaatcc aacaaattca
    20161 aatgagtagc caattttcat aaaactaaaa gcaaacgaga taatgatggg aatcaaaatc
    20221 gtcacaataa agaaaataac aaaaggtgtg accaatccat aagcactttt tttatgagac
    20281 gtatctgttg cttttttctt gctgttcttt attgtcttgc ttggtaacga agattgtatc
    20341 atcgttaagt cctcctaata ttttttaatc accagttaat tttacgccgt acttatgagc
    20401 aaaattttct gcttctttta gatatgaatt ttcaaaatca cctgtttgcc aatagttata
    20461 tgcagcggta gtcaaataag acgtagcatc tgaataagtg gctggtaaaa cagtatttga
    20521 tgtattttca ttgattttat tcaatagttc atagtatttt tgtcctcctt agtagtcaaa
    20581 ctttttctca gctgcttctg tttgataggc caatgagtta gatgtagcca cgcccagctc
    20641 cagtgcgttg gctaagcatt cttcatctgt cagcataaat gtaagaaatt gttgtgccac
    20701 ttgtggattt ttggcatctt tagggacata aaaacttgat cctcctgata ctgcaccttt
    20761 tcctggattt tcttttgaaa aaggaatcag tggagccatg cgccatttgt cagatgcatc
    20821 aggaaaattc aaagacatat tcgtagcata ccagccgcca gcaacaaaca ttgcagattc
    20881 ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt acaatatctg agtcgatcat
    20941 ttctttgatt aactttgcag catgtttcgc ttctttagta ttcagacgga tatttccttc
    21001 ttcatcaagc aaagattgat tctgctgggc tagcatgttg ataaacaatt cttggtcccc
    21061 agattctggc aaggcaattc caaaactacc agttttttct tttaatgctt ttgccacctg
    21121 gatatattct tcccaagaac tgatttcttc aaaatctaca cctatttcag caaatttttt
    21181 tggttgataa taacaaagtg ctggggtaaa atcactagcc caaggaataa tcgtttcatt
    21241 actcgtttgt tgacgcaaaa tattcaattt ttgatcataa aactgatcgc catatttgtc
    21301 ataaaatcca tattcatcag cactataaaa agcatcttga aatttttcca gcacacctgc
    21361 tgctgaagtg tctgccatgg agataatatc cggcatatct tcgtgtgtta ccaattttgg
    21421 ggtgatcatt tgtactgttg ctgttcccga agctacattc acaattttta agtcaatatt
    21481 tgctccagtt ttgcggttat agatttttgc agcattttca acatagtttg tcagtttgcc
    21541 gtttgcgaca tatacttcta atgtagtggc tttttttatc attggatctg cgatgtttgc
    21601 tgttacttct tcgttagttt ttccacatgc acttaagctg aataataggg taatgtttag
    21661 caaagctatc accaatttgt atcttttttt catgtctatc ccttctttct tttcaagttc
    21721 ttatataaaa cgctttcact tatgtatatt aacggaatta gtaacacaaa tatttatata
    21781 aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt tataatagtc attaattata
    21841 aatagaaagc tttttatata aaaagagaaa ggaaagtggt tcttttgtta acaaacaacc
    21901 aatcctccaa tcttgtcaaa aaaacactaa cctattccat agatacccat cttcatttat
    21961 ctgctgctcc ctattttcga ctactaataa atgaaagcgc taatttatat gctgaatttg
    22021 cacataaatc tatagaacta gataatcctt cccttttatt cattgataaa cagcaagaag
    22081 tccaaatctt ttctactcaa aaggaaacac aaacttttat cattatccag ttttattttg
    22141 atgagcaatt attagcacat attacgggac aatctttttc tatagatcaa gaaataacat
    22201 ctatcattac ttttctgaaa aatgaacagt tcaaaattga ttatctaaaa catagtaagc
    22261 cagctatttc ctatgatcag gaaatcaatt cttttttcat actatgccaa cagatgagca
    22321 ttcatttgct ttccttatta gttacttatt gtctgcaaaa aattcaattg tcttccttac
    22381 caaggcatta tcaacaggaa cttgctttat tgacaatacc ttcaaatgag aaagtcattc
    22441 ctaaaaaaaa gcatattctt tattcagatg aattagttga tccaatcata cgatatttag
    22501 aagaacatct ttcagaaaat tttttgcttg aacaattagc ccaaattttt tttatcagtc
    22561 cagccagctt aaaaaaacat tttagaaaag tgaatggaaa aagtgttatt agctatttta
    22621 aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga aaatgaatta acctatacag
    22681 aaattgcaga tatattagga tttcattcga tccatcattt ttcttcagct tttaaaaaat
    22741 atacagggtt atccccttca aagtattatc tatccttaaa atagaaaaaa atttttaggt
    22801 tttatccaca aattatttct ctatattttc tgtctattca aaataaacaa taattttccg
    22861 gcacaattat ttgctgtttt ttaccataac atagtacgat caacgtagaa aaggagatgt
    22921 ttttatgaaa gttgttgtca ttggcggtgt agctggcggt ccttcatttg ccactcgttt
    22981 ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa cgcggagaaa atatttctta
    23041 tgcaagttgt gctttgcctt attatttagg aggtgtgatc actgaccgtg actcgctgat
    23101 cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc gatgtcttta ctaaacacga
    23161 agtaacagca atcgatcctt ctactaagcg attaacagtt aaagacctat ccacaaatga
    23221 agaaataaaa acagattacg ataagttgat catctcttct ggtgctagac cagattatcc
    23281 ggatattccc ggagtttttg aagcagaaaa cggctttgtc ctccgtagtg tgacggatgc
    23341 ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa catgtcgtca ttcttggtgc
    23401 aggtgttatg ggtctggaat tagctgagaa tttcaagcat cgcggcttag acgtgacttt
    23461 aatcgatcaa ttgccacaag tcgctttccc ttatgatcca gaaattgcta atttagttta
    23521 tgacaaattg ctgaaagaag gattagccgt tcatttagaa acaagagtta ctgagatccg
    23581 tgataaaggt cgagaaatag tattatcaga tggctccgtc ctttctgctg atatgctaat
    23641 ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa gcaaccggca tacaattatc
    23701 agatacaggt cagatcattg ttgatgacca gttacaaacc aatctgccgg acatctatgc
    23761 gattggcgat attatcgaaa cgactagtgt agtgactggt cagccgatcc aaagtatgct
    23821 ttccagtgcg gccaatcgtc aaggacatat gctggcagat attttaaatg gtacgcctat
    23881 gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc tttgatcata cagcaagtta
    23941 tgcgggaatg acagaacatg aattaaaagc atcaggcata acaaattata aaactgtttt
    24001 tatcactccc tttgaccatg cctatttcta tccagaagct acaagattaa atctaaagct
    24061 gatttttgat gcagatagcg gtcgtatttt aggtggacaa gcatttggag aaaaaggtgt
    24121 cgataaacgg atgggagaac tttctgtagc aatcaccgga aacttgacag tctttgattt
    24181 gcctgatttg gagttgcctt actctccacc gtattctact acccgtgacc cgttgaatat
    24241 agctggttat gtcgcaatca atcaaatgac gaatatcgta gaaacgatca aagcaagtga
    24301 tatacccgaa aacgatttga aagaagcgtt ctttttagat atacgtgaac ctaataaagc
    24361 accttctggc agtatctcgg caacaaaaaa tattccaatg aatgagttac gagatcgaat
    24421 caatgaaatc ccaaaagata aaaaagttta cattactttc agaagaggat tgaatactta
    24481 tacttctgcc cgaatcttgg caggtttggg tatcaaagcg attttgattg aagaataaaa
    24541 aaacgaccgc ggtgggtagc cgcggtctag gagttatttt tacttgagga gtaaaaatga
    24601 aaaagtatat agggttgttg gttggtatgt atatagaata gaagataaat gtgaagaaat
    24661 tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa cattgcgcaa
    24721 tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg agacaaatca
    24781 tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat catattaatc
    24841 tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat ataataaagt
    24901 ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga tactaggaac
    24961 tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct cgttgtcttc
    25021 taatccttca atgaatgatc cctaatgtgt gtattaaata atttaatttt tttgttccta
    25081 tttatttaac a
//
LOCUS       DAZKAO010000030        24853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000030 DAZKAO010000000
VERSION     DAZKAO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.030"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(324..1106)
                     /locus_tag="ACG8H8_001009"
     CDS             complement(324..1106)
                     /locus_tag="ACG8H8_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986098.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="ACG8H8_001010"
     CDS             complement(1171..2007)
                     /locus_tag="ACG8H8_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8986099.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="ACG8H8_001011"
     CDS             complement(2025..3434)
                     /locus_tag="ACG8H8_001011"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8986100.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="ACG8H8_001012"
     CDS             complement(3448..5271)
                     /locus_tag="ACG8H8_001012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8986101.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="ACG8H8_001013"
     CDS             complement(6114..6440)
                     /locus_tag="ACG8H8_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986102.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="ACG8H8_001014"
     CDS             complement(6486..6848)
                     /locus_tag="ACG8H8_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP8986103.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="ACG8H8_001015"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="ACG8H8_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="ACG8H8_001016"
     CDS             7227..7562
                     /locus_tag="ACG8H8_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986104.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="ACG8H8_001017"
     CDS             complement(7638..9635)
                     /locus_tag="ACG8H8_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986105.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="ACG8H8_001018"
     CDS             complement(9733..10008)
                     /locus_tag="ACG8H8_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986106.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="ACG8H8_001019"
     CDS             10240..10974
                     /locus_tag="ACG8H8_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986107.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="ACG8H8_001020"
     CDS             11013..12155
                     /locus_tag="ACG8H8_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8986108.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="ACG8H8_001021"
     CDS             complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="ACG8H8_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP8986109.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13646..14116)
                     /gene="sufU"
                     /locus_tag="ACG8H8_001022"
     CDS             complement(13646..14116)
                     /gene="sufU"
                     /locus_tag="ACG8H8_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP8986110.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14103..15338)
                     /locus_tag="ACG8H8_001023"
     CDS             complement(14103..15338)
                     /locus_tag="ACG8H8_001023"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP8986111.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="ACG8H8_001024"
     CDS             complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="ACG8H8_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP8986112.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="ACG8H8_001025"
     CDS             complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="ACG8H8_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP8986113.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17604..18452)
                     /locus_tag="ACG8H8_001026"
     CDS             complement(17604..18452)
                     /locus_tag="ACG8H8_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8986114.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18476..19162)
                     /locus_tag="ACG8H8_001027"
     CDS             complement(18476..19162)
                     /locus_tag="ACG8H8_001027"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP8986115.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19155..20189)
                     /locus_tag="ACG8H8_001028"
     CDS             complement(19155..20189)
                     /locus_tag="ACG8H8_001028"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP8986116.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20252..20508)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20584..20922)
                     /locus_tag="ACG8H8_001029"
     CDS             complement(20584..20922)
                     /locus_tag="ACG8H8_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP8986117.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20924..21280)
                     /locus_tag="ACG8H8_001030"
     CDS             complement(20924..21280)
                     /locus_tag="ACG8H8_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP8986118.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21286..22473)
                     /locus_tag="ACG8H8_001031"
     CDS             complement(21286..22473)
                     /locus_tag="ACG8H8_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP8986119.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22664..23509)
                     /locus_tag="ACG8H8_001032"
     CDS             complement(22664..23509)
                     /locus_tag="ACG8H8_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8986120.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23743..24708
                     /locus_tag="ACG8H8_001033"
     CDS             23743..24708
                     /locus_tag="ACG8H8_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP8986121.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7264 a         5228 c         4223 g         8138 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    12241 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    12301 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    12361 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    12421 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    12481 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    12541 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    12601 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    12661 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    12721 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    12781 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    12841 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    12901 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    12961 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    13021 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    13081 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    13141 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    13201 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    13261 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    13321 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    13381 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    13441 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    13501 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    13561 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    13621 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    13681 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    13741 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    13801 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    13861 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    13921 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    13981 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    14041 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    14101 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    14161 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    14221 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    14281 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    14341 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    14401 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    14461 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    14521 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    14581 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    14641 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    14701 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    14761 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    14821 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    14881 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    14941 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    15001 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    15061 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    15121 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    15181 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    15241 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    15301 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    15361 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    15421 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    15481 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    15541 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    15601 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    15661 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    15721 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    15781 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    15841 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    15901 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    15961 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    16021 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    16081 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    16141 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    16201 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    16261 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    16321 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    16381 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    16441 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    16501 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    16561 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    16621 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    16681 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    16741 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    16801 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    16861 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    16921 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    16981 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    17041 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    17101 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    17161 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    17221 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    17281 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    17341 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    17401 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    17461 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    17521 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    17581 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    17641 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    17701 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    17761 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    17821 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    17881 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    17941 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    18001 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    18061 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    18121 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    18181 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    18241 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    18301 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    18361 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    18421 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    18481 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    18541 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    18601 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    18661 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    18721 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    18781 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    18841 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    18901 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    18961 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    19021 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    19081 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    19141 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    19201 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    19261 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    19321 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    19381 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    19441 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    19501 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    19561 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    19621 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    19681 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    19741 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    19801 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    19861 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    19921 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    19981 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    20041 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    20101 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    20161 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta taatttttca
    20221 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    20281 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    20341 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    20401 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    20461 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    20521 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    20581 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    20641 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    20701 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    20761 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    20821 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    20881 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    20941 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    21001 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    21061 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    21121 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    21181 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    21241 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    21301 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    21361 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    21421 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    21481 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    21541 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    21601 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    21661 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    21721 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    21781 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    21841 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    21901 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    21961 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    22021 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    22081 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    22141 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    22201 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    22261 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    22321 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    22381 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    22441 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    22501 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    22561 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    22621 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    22681 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    22741 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    22801 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    22861 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    22921 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    22981 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    23041 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    23101 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    23161 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    23221 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    23281 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    23341 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    23401 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    23461 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    23521 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    23581 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    23641 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    23701 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    23761 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    23821 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    23881 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    23941 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    24001 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    24061 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    24121 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    24181 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    24241 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    24301 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    24361 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    24421 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    24481 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    24541 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    24601 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    24661 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    24721 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    24781 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    24841 caagaggtcg taa
//
LOCUS       DAZKAO010000031        24677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000031 DAZKAO010000000
VERSION     DAZKAO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.031"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..812
                     /gene="purC"
                     /locus_tag="ACG8H8_001034"
     CDS             84..812
                     /gene="purC"
                     /locus_tag="ACG8H8_001034"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP8986122.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            812..1066
                     /gene="purS"
                     /locus_tag="ACG8H8_001035"
     CDS             812..1066
                     /gene="purS"
                     /locus_tag="ACG8H8_001035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP8986123.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1068..1739
                     /gene="purQ"
                     /locus_tag="ACG8H8_001036"
     CDS             1068..1739
                     /gene="purQ"
                     /locus_tag="ACG8H8_001036"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP8986124.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1740..3962
                     /gene="purL"
                     /locus_tag="ACG8H8_001037"
     CDS             1740..3962
                     /gene="purL"
                     /locus_tag="ACG8H8_001037"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP8986125.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3947..5386
                     /gene="purF"
                     /locus_tag="ACG8H8_001038"
     CDS             3947..5386
                     /gene="purF"
                     /locus_tag="ACG8H8_001038"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP8986126.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5409..6461
                     /gene="purM"
                     /locus_tag="ACG8H8_001039"
     CDS             5409..6461
                     /gene="purM"
                     /locus_tag="ACG8H8_001039"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP8986127.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6458..7036
                     /gene="purN"
                     /locus_tag="ACG8H8_001040"
     CDS             6458..7036
                     /gene="purN"
                     /locus_tag="ACG8H8_001040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP8986128.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7033..8568
                     /gene="purH"
                     /locus_tag="ACG8H8_001041"
     CDS             7033..8568
                     /gene="purH"
                     /locus_tag="ACG8H8_001041"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP8986129.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8584..9858
                     /gene="purD"
                     /locus_tag="ACG8H8_001042"
     CDS             8584..9858
                     /gene="purD"
                     /locus_tag="ACG8H8_001042"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP8986130.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9980..10894
                     /locus_tag="ACG8H8_001043"
     CDS             9980..10894
                     /locus_tag="ACG8H8_001043"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP8986131.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11027..12592)
                     /locus_tag="ACG8H8_001044"
     CDS             complement(11027..12592)
                     /locus_tag="ACG8H8_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986132.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12585..14036)
                     /locus_tag="ACG8H8_001045"
     CDS             complement(12585..14036)
                     /locus_tag="ACG8H8_001045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8986133.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14050..15066)
                     /locus_tag="ACG8H8_001046"
     CDS             complement(14050..15066)
                     /locus_tag="ACG8H8_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8986134.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15286..16374
                     /gene="chvE"
                     /locus_tag="ACG8H8_001047"
     CDS             15286..16374
                     /gene="chvE"
                     /locus_tag="ACG8H8_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8986135.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16428..17954
                     /gene="mmsA"
                     /locus_tag="ACG8H8_001048"
     CDS             16428..17954
                     /gene="mmsA"
                     /locus_tag="ACG8H8_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8986136.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17956..19161
                     /gene="mmsB"
                     /locus_tag="ACG8H8_001049"
     CDS             17956..19161
                     /gene="mmsB"
                     /locus_tag="ACG8H8_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP8986137.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19418..20395
                     /gene="guaC"
                     /locus_tag="ACG8H8_001050"
     CDS             19418..20395
                     /gene="guaC"
                     /locus_tag="ACG8H8_001050"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP8986138.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20659..22083
                     /locus_tag="ACG8H8_001051"
     CDS             20659..22083
                     /locus_tag="ACG8H8_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP8986139.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22125..22382)
                     /locus_tag="ACG8H8_001052"
     CDS             complement(22125..22382)
                     /locus_tag="ACG8H8_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986140.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22544..23653
                     /gene="pdhA"
                     /locus_tag="ACG8H8_001053"
     CDS             22544..23653
                     /gene="pdhA"
                     /locus_tag="ACG8H8_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP8986141.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23656..24633
                     /locus_tag="ACG8H8_001054"
     CDS             23656..24633
                     /locus_tag="ACG8H8_001054"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP8986142.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7988 a         4203 c         5579 g         6907 t
ORIGIN      
        1 agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat
       61 gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa ggaaaagcca
      121 aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta gatcaagcaa
      181 ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg aataatcaaa
      241 tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat tttattgaac
      301 aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg gaagtggtta
      361 tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat
      421 tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat ccatttatca
      481 atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt gagctgaaag
      541 cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc ggtttggttt
      601 tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt gcggatgaag
      661 taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg gacaaagacg
      721 tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat aaccgtctat
      781 cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat atgtaaccta
      841 taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg
      901 atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac ttgctgatgc
      961 agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg caaatgtaaa
     1021 tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg
     1081 tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt cgcgagatca
     1141 tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga tttgatgggg
     1201 ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg attgctcggt
     1261 tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca gtattcggta
     1321 catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta ctgagacgaa
     1381 atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc aagacggcct
     1441 ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat ggggaaggaa
     1501 attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag attgccttta
     1561 cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg aacaaagcag
     1621 ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg ctaggatcag
     1681 aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg accgtataga
     1741 tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc tatgccgaat
     1801 ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga agaatgccta
     1861 attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc tataaaaatt
     1921 ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa ggccctggag
     1981 aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc
     2041 acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt ggcggaatca
     2101 tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca ttgaggttcg
     2161 gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca ggtatcggcg
     2221 gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt gatccttgtt
     2281 atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac aaacatatcc
     2341 agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc
     2401 gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa gaagaacagc
     2461 aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt
     2521 tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg ggagcagcag
     2581 gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt atattgacat
     2641 tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg ttatcggaat
     2701 cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc caagaactat
     2761 ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac gggcaatatc
     2821 ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta gctgaagatg
     2881 caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt cagcagttag
     2941 ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg ctgcagcaac
     3001 cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta caaaccaata
     3061 cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg aataaagcgt
     3121 tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa atcggaggac
     3181 aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag cccttggcga
     3241 tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg gaattatcga
     3301 cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa
     3361 atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca atgattggta
     3421 tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa aaatcagggg
     3481 atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa ttacaaaaga
     3541 tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt gaaaagagaa
     3601 accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct catgactgtt
     3661 cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag ctagggatat
     3721 ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca agatttgtgt
     3781 tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat gctgctgttt
     3841 tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac ggtcagatca
     3901 aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt cttatgaagt
     3961 gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag acgccgctag
     4021 tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg caggtatcgt
     4081 gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt ctgaggtatt
     4141 caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac atgtacgtta
     4201 tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca aatttcatga
     4261 tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc ttagaaaaga
     4321 gttagaggaa aatggggcga tcttccattc taactccgat acggagatat tgatgcattt
     4381 gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt tgaatgaagt
     4441 caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg ctttggaccc
     4501 aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg tgattgcctc
     4561 ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc agcctggtga
     4621 gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg acacgcagct
     4681 ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca tcgcagggat
     4741 caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat cgccagtaaa
     4801 agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg gttatgcgga
     4861 agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg cccgaacatt
     4921 tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat ctgctgtacg
     4981 aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc ggggtacgac
     5041 aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc atgtgaggat
     5101 tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa cgcgtaaaga
     5161 actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg cagattcttt
     5221 aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag atgctccgta
     5281 ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat atgactatga
     5341 agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa
     5401 gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg
     5461 gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga
     5521 tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc
     5581 cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca atcgaagcag
     5641 acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat atcttagcac
     5701 aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat gatccgttac
     5761 gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat atggcgctga
     5821 ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat gatctagccg
     5881 gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg atcaacccag
     5941 gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac tctcttgttc
     6001 gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca gaattgaaaa
     6061 aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc
     6121 tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg tttgtagaaa
     6181 atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc tcatggccga
     6241 ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg gagatgtatg
     6301 aaatcttcaa tatgggcatc ggtatggtcc tggcagtcca agaatcgaaa gtaaaagaaa
     6361 tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca
     6421 aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg tttttgcttc
     6481 tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag gattggaatc
     6541 ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc
     6601 gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa aagagtatga
     6661 agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat tagctggata
     6721 tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca tcaatatcca
     6781 tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct ttgaagctgg
     6841 agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata ctggaccaat
     6901 catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt tagaagaaaa
     6961 aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg
     7021 aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg gattgtagca
     7081 ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg aacaaagcat
     7141 gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa
     7201 atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct tttaggaaga
     7261 cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc aatcgacttt
     7321 gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt cgaaatagca
     7381 gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac
     7441 catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact ttcagaatta
     7501 cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa agtctttcgt
     7561 catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt ggaagaaaaa
     7621 gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat
     7681 agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc gatcgcagca
     7741 gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc agatgctgcg
     7801 ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca catgaatcct
     7861 tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta tgaagcagat
     7921 cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct gccaacagca
     7981 gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac acaagaagct
     8041 ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt tgcgcaggaa
     8101 gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt acaagatcaa
     8161 gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa gccgactcca
     8221 gcagaagcaa aagcattagc gtttgcttgg agagctgtca aacatgtgaa atctaatgcg
     8281 atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat gaatcgagtc
     8341 ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc agttttagct
     8401 agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca tggtataaaa
     8461 gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat ggcgaataaa
     8521 tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag aggagtcgga
     8581 taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt
     8641 ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa
     8701 gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat ccattttgtt
     8761 aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc
     8821 gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc agcagctctg
     8881 atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat cccgacagct
     8941 aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg
     9001 ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt ggctaccaca
     9061 gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt tggggatagt
     9121 agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct tttggcgttt
     9181 gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg ggcatttgat
     9241 aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc gcaaatttca
     9301 aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa
     9361 gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac gaaggaagga
     9421 ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct
     9481 cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg tacagtagag
     9541 ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc cggttacccc
     9601 gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct ttctgtttac
     9661 tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg tctttattta
     9721 gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg
     9781 gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc tgccaatagc
     9841 gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca aacataatcg
     9901 gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt gttgtgtata
     9961 taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc agaaagcagc
    10021 tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa aagtgattga
    10081 gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg gaaacggtga
    10141 atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag aagatctttt
    10201 tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta aaaaacatat
    10261 cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg atgctttact
    10321 tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt ttgatgaatt
    10381 ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga
    10441 attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat ttggcttgaa
    10501 acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag caagtatcga
    10561 tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc aagcatggtc
    10621 accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt
    10681 aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg gcttggcttc
    10741 tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga tgaacgtagg
    10801 gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag attggtacga
    10861 attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag gtcaaaaggg
    10921 ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga tattctgtct
    10981 ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag ttatttttgt
    11041 ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt ttcttaaata
    11101 aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct gaaatacgat
    11161 agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc agataatcga
    11221 tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa
    11281 cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct ttgataaaat
    11341 ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag ggcagagcag
    11401 ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg
    11461 ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc ttttctttca
    11521 ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt gttcgagttg
    11581 gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt ttcgataatg
    11641 aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt ttcgcttctt
    11701 gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt tcaagtatca
    11761 tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg
    11821 cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt tgggctacct
    11881 gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc
    11941 gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac ttcaaataat
    12001 cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct aataatacgg
    12061 tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat gtttcactga
    12121 tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa
    12181 gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact gctttttcta
    12241 ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct gcctgtacct
    12301 ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg agacgagtcc
    12361 aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga
    12421 tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc atctcaccat
    12481 ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt atttgttccc
    12541 gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac atgtttgttt
    12601 cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc ttctcggtat
    12661 ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag acgcttgttt
    12721 acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc taactcttta
    12781 cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac tttgaattgg
    12841 atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg gacgtacttc
    12901 gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca
    12961 ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta tcttattttt
    13021 aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat ccagtcttgt
    13081 cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt cagagcttct
    13141 tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata aataaaatgg
    13201 ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta ttgtttcaaa
    13261 gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct agcggtaagt
    13321 gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc tgaagaaagt
    13381 tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc taaaaagcga
    13441 cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac tattgttaat
    13501 agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat ctccacacga
    13561 acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg attgatgatc
    13621 tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc gttttgataa
    13681 tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc tttaagtgta
    13741 tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat attttcctca
    13801 ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc caaaactttt
    13861 tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt ctgttgataa
    13921 atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac agcaaataga
    13981 agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata
    14041 cttctccagt caataatttc tggttgggat gttagctata ttctgctggg aaaagacagt
    14101 ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc caaaataacg
    14161 agtaacggta ttggctacat accagccgac atcaggttta cactcgacta cacaattgac
    14221 gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta caatgatcaa
    14281 atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt gagtcatttc
    14341 atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta aatatcgttt
    14401 catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa caatagtcat
    14461 cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat tgattgattc
    14521 caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag caagttctag
    14581 gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata aacccgcccg
    14641 atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg gatcattcgt
    14701 ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt cttttaatac
    14761 agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt tataggcaat
    14821 gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc ggtagagaac
    14881 ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt tccgccagct
    14941 actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt cctttgtatt
    15001 aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg
    15061 tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat aaaagggaaa
    15121 atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa
    15181 aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc
    15241 tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa aaacaaattt
    15301 agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag tgcgtgtggc
    15361 aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca
    15421 gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa actgggttat
    15481 aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca aatagaaaat
    15541 atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc ggctttaact
    15601 gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga tcgtttactg
    15661 atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt aggtgtatcg
    15721 caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc attcacgatt
    15781 gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa cggagtgatg
    15841 tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg acagacgagt
    15901 ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag gatggataat
    15961 ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca
    16021 ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga aagtaaacct
    16081 ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat cattgccggg
    16141 gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac gattgaaatg
    16201 atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac ttatgataat
    16261 ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa agataattat
    16321 caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa ttaagaggaa
    16381 ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg gcggattgta
    16441 ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta gattgcgtga
    16501 atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat
    16561 caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac ggggaaatct
    16621 tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa aaaggcatcg
    16681 tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag aatatttttc
    16741 tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa aagaaaacat
    16801 atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta tcccagattg
    16861 gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt
    16921 tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat ttgctgagat
    16981 tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat aagctgaatg
    17041 aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg attgagacac
    17101 tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc agagatttag
    17161 ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa gtagaagact
    17221 ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc aacttgcatt
    17281 tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga actgaattcg
    17341 ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata ttaaaggaag
    17401 gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc gcttatgtct
    17461 cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa aacgtgacgc
    17521 tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa gaagtaactg
    17581 aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat caaattgtca
    17641 gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg atgacacaac
    17701 ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca aaatatgaga
    17761 tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt atctcttctg
    17821 aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa ggacgtatta
    17881 caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta atgacgaaag
    17941 aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta
    18001 aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat cattttagcg
    18061 ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc attgaatata
    18121 actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat gttattagtt
    18181 gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt
    18241 gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat cgcatgtctt
    18301 gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc agggataccg
    18361 gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca agttgttcta
    18421 ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac gggttattta
    18481 ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg aatgatcatc
    18541 acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa tcatttattt
    18601 gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt cgttgtacta
    18661 ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat cattcttggt
    18721 ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg acagatttat
    18781 gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg
    18841 ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt tttagctgct
    18901 cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc aatggcggca
    18961 gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg tgcgattgtc
    19021 ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt cggggtagac
    19081 tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga cttgtataat
    19141 aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact gtgacaagac
    19201 atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt gtatagacta
    19261 aaaataatat atgttttttt accattcgta taaattctct ttttgctctt gatttttttg
    19321 cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt aataatgttc
    19381 gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga
    19441 tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg atacaacggt
    19501 cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat
    19561 tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt
    19621 tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc
    19681 gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc
    19741 gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact cagttattga
    19801 tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg
    19861 tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat
    19921 tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca
    19981 actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg
    20041 gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat
    20101 tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat
    20161 cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt
    20221 tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat
    20281 gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg
    20341 aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat
    20401 aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa
    20461 acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt
    20521 tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt
    20581 ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga aaaaatagac
    20641 aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt
    20701 caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa
    20761 cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac cgttgtccat
    20821 gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag cggatggttt
    20881 gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg gcaaatgatc
    20941 tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc aaagcagatc
    21001 caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct tttgccgaat
    21061 tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga aatggatctt
    21121 ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa atttgaattg
    21181 gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat ctttttgatt
    21241 ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc ttttttttac
    21301 cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat ggatgaagaa
    21361 gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa agcagtgctt
    21421 atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg acgagaaaag
    21481 ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt atttacggat
    21541 ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc ggataatgtg
    21601 ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata
    21661 ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga gttttcgatg
    21721 aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga tttgaaaatg
    21781 caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg gggagattca
    21841 caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat tacttgggat
    21901 gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct tggaattgca
    21961 ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag taggctggat
    22021 gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg gttaacagaa
    22081 taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat ttcaagctgt
    22141 ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc attatttgta
    22201 cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat aacaccaacg
    22261 tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa
    22321 atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat gcggattggc
    22381 ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat aaatccatga
    22441 taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt tcaatcgcag
    22501 aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa acaaaaaagc
    22561 accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag tatatcaagc
    22621 acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat ccgatgatga
    22681 actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac gttcaacagc
    22741 gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag aggcaagtca
    22801 gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt atcgggatgt
    22861 acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt ctcgaggaca
    22921 tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg
    22981 tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa
    23041 tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat
    23101 caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca acgggtatgc
    23161 gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc
    23221 agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat atactgtatc
    23281 taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg aaacactgac
    23341 ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc gtacaaaaga
    23401 cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat atttgacgga
    23461 aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta aagaagaaat
    23521 caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag atttcttgaa
    23581 aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt atgaagcgaa
    23641 ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga tgccttagca
    23701 cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga
    23761 ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg agtattcgat
    23821 actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt
    23881 cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga cgaagtagta
    23941 gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc catcacaatt
    24001 cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa tttagaaggt
    24061 ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta tgatgcaaaa
    24121 ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga acacatgaaa
    24181 ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc tttggataaa
    24241 gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc aatggtgcgt
    24301 gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga aatcattgat
    24361 cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga aaaaactggt
    24421 cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca agtagtttct
    24481 gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt ttctgcacct
    24541 gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc aaaagatatc
    24601 gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa caaagtcatg
    24661 gcttatcaat ttaaatt
//
LOCUS       DAZKAO010000032        24411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000032 DAZKAO010000000
VERSION     DAZKAO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.032"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..461)
                     /locus_tag="ACG8H8_001055"
     CDS             complement(93..461)
                     /locus_tag="ACG8H8_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP8986143.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            632..1588
                     /locus_tag="ACG8H8_001056"
     CDS             632..1588
                     /locus_tag="ACG8H8_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8986144.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1661..1777
                     /locus_tag="ACG8H8_001057"
     CDS             1661..1777
                     /locus_tag="ACG8H8_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP8986145.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1832..3514)
                     /gene="recN"
                     /locus_tag="ACG8H8_001058"
     CDS             complement(1832..3514)
                     /gene="recN"
                     /locus_tag="ACG8H8_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP8986146.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3532..3981)
                     /locus_tag="ACG8H8_001059"
     CDS             complement(3532..3981)
                     /locus_tag="ACG8H8_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8986147.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4088..4906)
                     /locus_tag="ACG8H8_001060"
     CDS             complement(4088..4906)
                     /locus_tag="ACG8H8_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP8986148.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4915..5805)
                     /locus_tag="ACG8H8_001061"
     CDS             complement(4915..5805)
                     /locus_tag="ACG8H8_001061"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP8986149.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5807..6031)
                     /locus_tag="ACG8H8_001062"
     CDS             complement(5807..6031)
                     /locus_tag="ACG8H8_001062"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP8986150.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6036..7373)
                     /gene="xseA"
                     /locus_tag="ACG8H8_001063"
     CDS             complement(6036..7373)
                     /gene="xseA"
                     /locus_tag="ACG8H8_001063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP8986151.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7374..8228)
                     /locus_tag="ACG8H8_001064"
     CDS             complement(7374..8228)
                     /locus_tag="ACG8H8_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP8986152.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8325..8774)
                     /gene="nusB"
                     /locus_tag="ACG8H8_001065"
     CDS             complement(8325..8774)
                     /gene="nusB"
                     /locus_tag="ACG8H8_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP8986153.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8767..9192)
                     /locus_tag="ACG8H8_001066"
     CDS             complement(8767..9192)
                     /locus_tag="ACG8H8_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8986154.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9438..10502)
                     /locus_tag="ACG8H8_001067"
     CDS             complement(9438..10502)
                     /locus_tag="ACG8H8_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP8986155.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10674..10919)
                     /locus_tag="ACG8H8_001068"
     CDS             complement(10674..10919)
                     /locus_tag="ACG8H8_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8986156.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11058..11432)
                     /locus_tag="ACG8H8_001069"
     CDS             complement(11058..11432)
                     /locus_tag="ACG8H8_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP8986157.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11515..12429)
                     /locus_tag="ACG8H8_001070"
     CDS             complement(11515..12429)
                     /locus_tag="ACG8H8_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8986158.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12450..13253)
                     /locus_tag="ACG8H8_001071"
     CDS             complement(12450..13253)
                     /locus_tag="ACG8H8_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP8986159.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13286..14275)
                     /locus_tag="ACG8H8_001072"
     CDS             complement(13286..14275)
                     /locus_tag="ACG8H8_001072"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP8986160.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14378..14869)
                     /locus_tag="ACG8H8_001073"
     CDS             complement(14378..14869)
                     /locus_tag="ACG8H8_001073"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP8986161.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15060..17867)
                     /locus_tag="ACG8H8_001074"
     CDS             complement(15060..17867)
                     /locus_tag="ACG8H8_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8986162.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18301..18594)
                     /gene="rpmA"
                     /locus_tag="ACG8H8_001075"
     CDS             complement(18301..18594)
                     /gene="rpmA"
                     /locus_tag="ACG8H8_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP8986163.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18607..18951)
                     /locus_tag="ACG8H8_001076"
     CDS             complement(18607..18951)
                     /locus_tag="ACG8H8_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP8986164.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18978..19286)
                     /gene="rplU"
                     /locus_tag="ACG8H8_001077"
     CDS             complement(18978..19286)
                     /gene="rplU"
                     /locus_tag="ACG8H8_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP8986165.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19306..19382)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19568..20209
                     /locus_tag="ACG8H8_001078"
     CDS             19568..20209
                     /locus_tag="ACG8H8_001078"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP8986166.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20271..21902)
                     /locus_tag="ACG8H8_001079"
     CDS             complement(20271..21902)
                     /locus_tag="ACG8H8_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP8986167.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(22025..22384)
                     /locus_tag="ACG8H8_001080"
     CDS             complement(22025..22384)
                     /locus_tag="ACG8H8_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP8986168.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22543..22616)
                     /locus_tag="ACG8H8_001081"
     tRNA            complement(22543..22616)
                     /locus_tag="ACG8H8_001081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22580..22582),aa:Arg,seq:tct)
     gene            complement(22925..23608)
                     /locus_tag="ACG8H8_001082"
     CDS             complement(22925..23608)
                     /locus_tag="ACG8H8_001082"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP8986169.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23736..24254)
                     /locus_tag="ACG8H8_001083"
     CDS             complement(23736..24254)
                     /locus_tag="ACG8H8_001083"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8986170.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT         7146 a         5325 c         4019 g         7921 t
ORIGIN      
        1 ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc aaaaagcctt
       61 ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat gttcacaatg
      121 atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt catcttgaaa
      181 taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat acaaattccc
      241 attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt atttctagac
      301 aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt tattttaaaa
      361 cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac gattaccggg
      421 taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa ctcctttttt
      481 atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta tttaaattta
      541 tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat
      601 aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga
      661 gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc
      721 taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac
      781 ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac
      841 aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt
      901 tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat
      961 tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga
     1021 tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa
     1081 cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc
     1141 cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag
     1201 agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca
     1261 agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat
     1321 gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat
     1381 cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga ttattttaac
     1441 tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag
     1501 ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg taactaccta
     1561 ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg aaaaaagatt
     1621 gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa aaaaacaaac
     1681 aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt taacgattgg
     1741 atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag ctatcagctg
     1801 ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt ttcaatagtt
     1861 cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata cgtgcaattt
     1921 cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt ccatcaatca
     1981 ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc agatgagtga
     2041 tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg gcctgtgcaa
     2101 ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca ccttgctcag
     2161 aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca gaagcgactt
     2221 tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg atttggtcaa
     2281 atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca aactcagtgt
     2341 tttccatata cagacttttc aattcatgca gaatggattt ttccaattct tttgcgattt
     2401 ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct ttttgctcta
     2461 gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct agctcttcgg
     2521 tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta cgtttcaact
     2581 gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct aactccaata
     2641 aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa gcactttgga
     2701 tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc agttctgaaa
     2761 cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct ccgtaacctg
     2821 cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct tcttttaatt
     2881 tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg aaatggagca
     2941 tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc cttttttcca
     3001 gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg aaagctacat
     3061 ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc tgatgttcgt
     3121 tttgtccttg gatatctact aaataactgc cgattcttct cagattagct aatgtgataa
     3181 tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt cgaacaatca
     3241 gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat ccttcttgtt
     3301 ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt atataatcgc
     3361 ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat ttacccgcac
     3421 cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat tccggaataa
     3481 tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa cttaaaggta
     3541 atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa tcatcaaaac
     3601 attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat caatcaaatt
     3661 tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt ccatttggtc
     3721 aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat cttcgtttgt
     3781 ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga tcagtttcag
     3841 ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc ttttcaggat
     3901 ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag taatcaaacg
     3961 atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct taatcaatga
     4021 tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg attttcacag
     4081 ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct gccatattaa
     4141 tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag aactcgatat
     4201 ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat ccaccttgtg
     4261 ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca cgaacaattc
     4321 cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa gcaatcactt
     4381 ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt gaaataaaag
     4441 aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt tttgcataac
     4501 gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc caagctagct
     4561 gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa gctgcatcag
     4621 taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca gatagatcaa
     4681 atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga agttttttac
     4741 ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca aaccgctcgt
     4801 ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct cttgtttcaa
     4861 ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt tccctcattt
     4921 ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag aaaaaacgtc
     4981 ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca acgcctcacg
     5041 tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt catctttacc
     5101 agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat ctcttatctg
     5161 aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt cttcaggttg
     5221 cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg tctttctttc
     5281 atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga tatctgcagc
     5341 ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa gcagcagaag
     5401 cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg taagcaaacc
     5461 atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag gttttcctct
     5521 acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat acgtatgcac
     5581 catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat caaaggaagc
     5641 aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca ctgaataact
     5701 catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca agatttcttt
     5761 ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag ctttcctcgt
     5821 ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag gtctcttctg
     5881 ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta aacgaatcta
     5941 gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt tctaattctt
     6001 gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc tatctcttgt
     6061 acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc ttgatgaatc
     6121 tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc tcttcccatg
     6181 attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg ctgctgctgt
     6241 tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc agtttcttgg
     6301 cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct taatgcgatc
     6361 gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg ctgtttcaac
     6421 tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa tacataggaa
     6481 cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa cgcctgctgc
     6541 aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg aacagctaat
     6601 tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc gatcgtggta
     6661 tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat cgcccgagca
     6721 acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg accgatgatc
     6781 attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac attgtccgct
     6841 gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg atatcttctt
     6901 ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag gaccgcgatt
     6961 cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc cttcatcagc
     7021 ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg ctccatgtgc
     7081 tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc aacgacaagg
     7141 actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt gaacataata
     7201 gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt ggcacgtaaa
     7261 cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc ggcatcaaat
     7321 tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc catctattca
     7381 cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt gatagtcatt
     7441 ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac ttcatcaaat
     7501 ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc tatcactaca
     7561 gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc tacgactaaa
     7621 atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc aacagtaaca
     7681 gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt gcttcgtccg
     7741 ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat tttcatgatg
     7801 ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc taaattcatc
     7861 ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt ttcttcatct
     7921 atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc ttgattgtat
     7981 ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg ctctaccaat
     8041 gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat ttggctggct
     8101 ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc tttcaatttt
     8161 tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc gttaatcaat
     8221 tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata gaattttccc
     8281 gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc cccagcttct
     8341 aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg gtcatcacta
     8401 aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac atctgtcaca
     8461 taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga gatccgattg
     8521 atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc tttttttgca
     8581 cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc atcttcgttg
     8641 atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt tgttaggtct
     8701 gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc gtgtcttgat
     8761 aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg ttggttgttc
     8821 taatttttca ggaacaacag ctactacatg gatattgact tcagataaat cgatatccgt
     8881 catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt taggaactga
     8941 aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct cgtttgctaa
     9001 gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc tggcaaaagt
     9061 tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa ttacttcgat
     9121 gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa tcaaattttt
     9181 ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc cgaattctct
     9241 atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg caaactgcta
     9301 tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc ttctatagtg
     9361 tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt ttacgcttac
     9421 ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt caacacacga
     9481 tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat tcctggtaag
     9541 tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc tgcacgagaa
     9601 gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga atgtccaaat
     9661 gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa ttggacacca
     9721 gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac tatttgataa
     9781 atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat atcggaaacg
     9841 tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc gatgattttt
     9901 ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat cgtatcaaaa
     9961 gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc gacttcaatc
    10021 tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc cgcaatatga
    10081 catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag ttcttcgatc
    10141 atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga atattctaaa
    10201 aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg tactaagttt
    10261 gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg agcctgcgct
    10321 gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa agtaataaca
    10381 gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg actggtaacc
    10441 aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac acgtagcatc
    10501 ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact tttcttcgta
    10561 cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa ataaagccaa
    10621 aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa aacttatgat
    10681 cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa aatagatgga
    10741 ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg tccaatactt
    10801 gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc tttactggtg
    10861 atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa ccaactcata
    10921 atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg gacaaataat
    10981 tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa cctcctctta
    11041 gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg tttcactaca
    11101 tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct gatcgcacga
    11161 agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt cgtttgaatg
    11221 gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata atccacttct
    11281 tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc aaaaccatta
    11341 ttacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc atcaattacc
    11401 aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt tgtttatgaa
    11461 cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta tggattataa
    11521 aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga taacgattgg
    11581 agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg cagctaaacc
    11641 aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag aaagggctaa
    11701 gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag gcaatttatc
    11761 ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta agaatttgat
    11821 tgatacccat ctttgtacaa gggcagctaa tacgaacatc cctaaaattg aagcaccttt
    11881 tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac cagctttgta
    11941 gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac ctaagaagaa
    12001 gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg cacctagcat
    12061 tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac ccatcatacc
    12121 tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac gttcttcttc
    12181 cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat taaagaattc
    12241 caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt ttttgattgc
    12301 tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc atgaaccttg
    12361 aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt tgattttttc
    12421 ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc gcctaaaggg
    12481 tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga aaggttcaag
    12541 taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa ttgagaaatt
    12601 gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc catcatgttg
    12661 attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat accatctgtt
    12721 aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat aaatagcaac
    12781 gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat gtgccacatt
    12841 tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac gattggaaca
    12901 gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg aacggcaatc
    12961 gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat aattgctgat
    13021 gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc ccaacctaaa
    13081 gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc ggtaactaaa
    13141 ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag aataccttcc
    13201 ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga catgataagc
    13261 ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt gcttttttca
    13321 aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg aatttcacac
    13381 ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac aatactttac
    13441 ttacaacgac tttaccgacc gaatgcgcca ttgacccaac atttaattct ttgatgtcta
    13501 cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc aatgctttgg
    13561 tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga ataacatttg
    13621 cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca tcctttgata
    13681 ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa gctgtcgcaa
    13741 cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt tttccgtctc
    13801 cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact gctgctttcg
    13861 gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt gctgtttcaa
    13921 tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg taggcttcaa
    13981 tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc tcttcgaata
    14041 atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat aacacttcgt
    14101 cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca tctggacctt
    14161 cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc attgaaccgg
    14221 attgtaagat tccttcagca aattgtccat gacttgctag gataatccct accatattta
    14281 tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc taaccattac
    14341 aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg attttcgcta
    14401 gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct aactccactt
    14461 cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa acaaaattgg
    14521 taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac ttcaactgta
    14581 caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc atcacttttg
    14641 ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt attacatttg
    14701 ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta tcttgagcga
    14761 ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa actgttgcga
    14821 cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc tgcaaaactc
    14881 cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac cacctcctag
    14941 cacacactac tataagcaaa aagcgtgcca acttttttac gatacactaa ttgaatttaa
    15001 aggattctta accaatacac aaaaacgata cactataaaa gtgtatcgtt tttgtgtatt
    15061 catggcacag tgtattcttt tttatctaac tgattatgga ctagacgtag cagataatac
    15121 atttcttcta tcggcacttt tacttttatc tgatcttcca acagaacaac tgaggctgcc
    15181 aattggctat aaagaggatc attcgttgta tttgtcaatt cgttttcttc cacagtcaat
    15241 ggttcattcc gaataatacg ttcgatcatt cctgctgtgt gcatcactag gttgattttg
    15301 aatccatatt cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga aaattgccac
    15361 attggatcga ttaacttact accattaata aagatgaagt tatcagagat aaactcaaca
    15421 cacgtatttt tagctgactc ttcatctagg gagatgaatt cagtttcctc caattctgat
    15481 tccatcagca actgatccaa aatgacttcg atattttgat caataaaacg ttctaaagga
    15541 ataaatgggg cttggatatt caaatcagta atcccagtag tagctatgat ctgatattct
    15601 ttttggataa ctggcagttc ttctttcact tccacaatcg acaaagctag aacttctaat
    15661 tctgtttctt gacgcttgtt taccgctcgt tcgatcaatt ctttgattct ttgtgctgta
    15721 ccttcgccag atgcgcagat agccaaaaca gcttttttct tatgaatagg ttttgcttcc
    15781 tgaacagatt ctacagaaga tatttctgcg taacctcgaa aatttttcaa tgattcatgc
    15841 aacatatcta gatctgtccc taatactgag acctttcgta cagtttccaa aactagagaa
    15901 gttgttacca tatcaacagt acgtactgga attccagtgt ctctttgaat ttgcgaatta
    15961 aacgacgcaa gggaccccat atctactaag agtatgaccc cgcttccttc atctacttct
    16021 tgtacattgc gttcaattcg gtccaatgct gttttcggat ccatatccaa aggcatgtct
    16081 actgctcgta cgttctctgc atctaacaac tgttggacaa cttggaccat actgcttgct
    16141 gtgctgtttc cgtgagctgc aacgactaca ccgattctgc cgcttgcttg atttgcccgt
    16201 aacgagacga gtagaacggt caagtaatag tcttcacttt ctggaatctc cacttgaaaa
    16261 tacgtttcga tataactacg cacttctttc gcaacagcat attcatctgg aaaatcaaaa
    16321 gccatctcac ggatgttatc attcgtatgc cgttcttctc ctaagttgat ttttttcaaa
    16381 aaagaactga tatgcaagct catagcataa ataaagtttt gctgaaactc atatggtaaa
    16441 gatcttttga ccattacata aatctgattg gtcacttcta tgattttcgg atctacaaat
    16501 tctgctaatt tgctgtctgc attaaaagaa aaaccatggt ctttatagaa agacttcaga
    16561 tggatgttga tatctgtaga aatgaaatga ttgattgctt cttgatccaa accatccgac
    16621 ttcagtaaag ctgctttatc tccgatgata tcatatagat tataaggtaa ctcataggag
    16681 tccgattgga ttttcatgat cgtatcattt ggtgctacag ttatctttgg ctccaatatt
    16741 ttagaaagct ctgccattgc actgcggttg cttgctaatt gaatcaatcc gctacgaatc
    16801 ccttctgtca aatcatcgat tgtgatcgag atttcgggtg attgcatatg atttagaaat
    16861 cctcgtgcac atactaactg gatatttgac ttcaattgtc ctatatttcc ataggtcaca
    16921 ctgccgatca atgcttttgc tacatcttct gtcaaagaaa tctttcgctg cgtgcggttt
    16981 gcttcgtggg caatcatgat ttttactaaa tcgactttct catttgcagg acgtttttca
    17041 aaagccggaa gtttgatatt gattgggata cgccgaacaa aagtttctaa tagagaagaa
    17101 cctgggtctt ctgtggtcgc acctacgatc cgtacatttg cttcatggga tttcgttgtt
    17161 tccccaagac gactgtaagt tccatgatcc ataaagtaaa agatcatttc ttgaccttca
    17221 ggcggcaacc ggtgaatctc atccaaaaac agcataccgc catccgcttg atcgataatc
    17281 cccatttttt cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa taaatgactc
    17341 atcaaaagct cagggttatt ggcataatca gcacaattga aaacaatcag ttccgtttcc
    17401 tcatctatta catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa ataagtcttt
    17461 cctgatccgg taggtcctgt aatcaaacaa tttaatcctc ttggcggata caaaatcgca
    17521 gcttttgcct gttccactga atttttcatg ctgccgttag ctccaatgat ttttgcaaag
    17581 atatccttag tatctatttg gagtgtattt ttttcttcag agaccggcgt ttctttgtaa
    17641 ctgactacat gttttgtaac tggctttacg tgcgtaacga gtgtttgata tatgtaacgt
    17701 actggtcgtc cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt cagatcttta
    17761 ctgctatttg ttcgctgaat ccctaacact tcagcaattt cctgtgttgt tactccttga
    17821 tcatattctg cctttgttaa atgagcggtt ttttctttga catacatata aattcggtca
    17881 atgcgttttg ccaatggtat cgctcctttt ctatagacta ttgtacttag gcttcaaaac
    17941 ataatactat gtatttattt ctgtttcgtc aactatttac ctgattttgt tttgttgata
    18001 acacgcggat agatagaaat gttcaattta aaagaacgaa aaatcccgat acgattttta
    18061 taatggaaac aagaaaaaga acactgttta ccatataatt atttcactga tagcaaacaa
    18121 tatagcttcc atcaattgaa aacgatagtt atcaaaacat cctgattcca aattggcaaa
    18181 aaaatattaa gtgtaaaaag acaaagtaaa cttcgtagcc tccactacaa cttttatttt
    18241 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattcaa
    18301 ttaagcttct tgagctactg gataaacaga cacttgtttt ttgttacggc ctttacgttc
    18361 gaaacgtact acgccatcaa cttttgcaaa taaagtgtcg tctccaccga tacccacgtt
    18421 tacacctgga tagatttttg taccgcgttg acggtaaaga attgatccac ctgtaactgt
    18481 ttgtccatca gcacgtttag cacctagacg ttttgattct gagtcacggc cgttagatgt
    18541 agaaccgcca ccttttttgt gggcaaataa ttgtagattc atagataaca acatggtctg
    18601 cacctcctat ttatcattaa ttgttttaat ttggatatat tctgagtttt cagcttctat
    18661 cgattgtaat cctaataaaa gattttctaa tagaatctca gcaatattgt tttgttcttg
    18721 tgtcattcct gaagtgactt cggtataaag ataaccgcct tcttcttcat tcatttcaac
    18781 aatcggctca aaaccggcta atgaggcaat cccgttgaca gtgctgatcg ctaacgcaga
    18841 aacacccgca caaacgatat cacttccata aggaccagca tcagcatgcc cggtcaaagt
    18901 gaatgaaacg atctgacctg cgtcatttcg cttaaatgtt cctttgatca tatataggcc
    18961 ttctttctca aacgctatta agcgttgatc gcgtcgataa ccacttttgt atatggttga
    19021 cgatgacctt gtttacggtg tgaatgtttt ttaggtttgt atttgaaagt aacgactttc
    19081 ttttgtttgc cgtgtttttc aacagtacct tcaacagttg cacctgcaac agttggagcg
    19141 cctacttttg tagattctcc gcctactaag ataacttcgt caaaaacaac tttttctcct
    19201 gcttcaacgt caagtttttc aacatagatt gcttgtccca cttcaacttt tacttgttta
    19261 ccaccagttt taataattgc gtacatgcta taaagcacct ccttctgata tacttagact
    19321 cgccatccga agtgacagca tatgctgtgc ttattacttg ttctgagcgg ttgtagctgt
    19381 ggagtccaca attacaacag tactagctta ccagaacagc aaccgttagt caactaaatt
    19441 taaaatcaaa aagtaaaaca aaaattcaag caatacttct gcttttctat cctcacagtt
    19501 ctaccaaata aaatagtcgc attgtgtttc ttttattctt ataataaaag aaaaaggagg
    19561 agcataaatg gaatacccaa aagaactgat acaagaagct tctttaagaa tgaacggccg
    19621 tcctgtagaa ggatttatcg ctgggcaagg acctcttcat cctaaactga tgctcgtggg
    19681 agaagcccca gggaaaacag agattgatac gcatgttcct tttagcggtc aagctggaaa
    19741 agaattgatg caggcacttt cttcaactgg cttaacacga gaagaggtgt atatcaccag
    19801 tgctgtaagg agccgcccgt atcgcgtgac tcatcggatc aataaacgga cacaacagtc
    19861 agaaactgtt tatccaaatc ggacacctac tcgttccgaa gtatttgcgc atgcaccaat
    19921 attggattac gaactgatga aggtccagcc aaaattaatt gcgacacttg gaaacattgg
    19981 tcttcaacgt ttacttggca aagaatatac ggttaccaaa aatcatggtc aactctatac
    20041 agggcctgtc ttacaattaa cagaacaaaa ggatgcctac gaatggtcag aaaaaaacta
    20101 tcgaattctt ccactttttc atccagcagc tattttctac aatcgctccc ttgtacctgt
    20161 tatccatcaa gactggcaag tactagcttc acttttacag caatattaaa aagaacagac
    20221 aagaccataa aatcttcagt ttacagtctt gtctgttctt tttcattgtt ttaatggcta
    20281 attgcagtat atttcgcatg taaagctgtc aacgcatgag caactgtttg atgttgatgc
    20341 gtgaatgtat gttcccacaa gtgagaaagt tgtttttcta cttctggctg caaggaagta
    20401 tagataatcg tttgtcctct ttgctctgct ttttcttgaa attcatcaaa aactgcacgt
    20461 gccgtcaaat caatacttgg tacgcctcgc atggagaaaa tgacaccatc gtgatgattt
    20521 agttcttcca atttattttt gagtttttct gaagacataa aaaacagtgg accactaata
    20581 tacactactg tccagttatt cagcttttcc gtttctggca gccccattct ttgccaatcg
    20641 atcgtttcta atgaaatcgt gatagcagcg cttttgacaa caaaaaatac acagccgccg
    20701 atcaccccga tcacgatagc aatacttaaa tcaaagataa ctgtacaaat catcgtaagg
    20761 ataaacaata gaactgctga ccaatattta ttagtaaata gctccttgat cgtttcccat
    20821 tcattcatac gccaagccgt cacaatcaaa acacctgcaa gtgctggcat tggaatctgt
    20881 gacatcactg gcgcaaaaac aagcatggag agcagtaaga caactgcatg tatcatacca
    20941 gctaatctag tttgtgcacc agatttgatg gccacgcttg tacgggcgat ggccgcagta
    21001 gcaggaatcc cgccaaagaa aggaagcaac agatttccaa ttccttgcgc cattaattct
    21061 tgattgctat ctaagggctt atttgccatt cggccagccg aagcaccaca tagcaagctt
    21121 tcaatcattc ctaataaagc aatgctaata gctggaactg ccacttgttg caaggcagaa
    21181 aagctaaaat ctcccatatt caaacgatta ctactgatca atgtttgtgg aatcttaccc
    21241 acagttgcta ctggtaaatg gaacaatagc ataactgctg ttgccaaaat gatagccagc
    21301 agcgaactag gcattcgttt cgcccatttt tttggataga taaacatccc taaaatgact
    21361 agccctccca tgatcaaaga ggctgtgttt atcggaaatc ctatcgtttg ataactactt
    21421 agtttttcaa taacgtttgt accttccgag tgaacaccaa ataaattatc gatctgacct
    21481 aatgcaataa tgatagcaat acctgaagta aaccccgtaa ttacgggtgc tgggataaaa
    21541 gaagtcagtg ttcctaatcg gaaaagtcct gctgcaagta aaaatagacc cgctaaaaaa
    21601 gtagcaagca atacaccttg cattcccttt gttgccgcaa tagacatcaa gatagctgcc
    21661 atcgcacctg tcggacctga tatttgataa aaaccgcctg ataaaccgcc aataacgata
    21721 ccggcaatga tagccgtaat caaacctgcg gccgcatcag ctccgcttga taccccaaaa
    21781 gccaaggcta aaggcaatgc tacagcagct actgtgatcc cagccaaaag atctttctga
    21841 aatcttgaaa gatcataacc tgaaaattct ttttttagtg aggaaacata atttttgaac
    21901 acctatctaa ctcctttttc atgaaatttt ctaaaatacc attcattaga atatcatatt
    21961 tcatgagata aagtaatatt tttcacaaag tttcctgtta gataaattct tattttttct
    22021 atatttataa gaaaatagaa agcaatgcca acagagctag cccaccttgt ttgagtaaaa
    22081 tccaacgatc gctagtcaaa ctgccatata atgcaacaaa aaaaatagat attaatagca
    22141 tacttgtgat ttctttagat gcggaagaaa gataagctcc ataaagtagt cccactccta
    22201 atagtccatt gtacactcct tggttcttaa acaatgtttg aacagatttt ctgcttaact
    22261 cttctttact cattttaaaa accctacttg tcgtgtcaga atcagttctc accgtttcga
    22321 tatacatgat ataaaaaaat tcaattgcaa ctagagtcac taaaattcta gatagtatcg
    22381 tcatatcttc ttcctctttt ctgatgagtt ttattttttt gctccttata attgtactcg
    22441 ataaggatgt ttttttattt tatttgcctt tgaaaacaaa caatattggc acaaaaaaca
    22501 aagaaaaacc gttagaaacc tcagtttcta acggtttttt agcgtcacag gagggattcg
    22561 aacccccgac cgtccgctta gaaggcggat gctctatcca gctgagctac tatgacaaga
    22621 atcagcggaa catcaataat tatagataat taaattgctt ctgtcaatac gaaatagtta
    22681 tttaattagc aaataatgaa ttattttggc ccaactacta aatcaatctt ttaaaacgag
    22741 acattctcct tcagctgttc taaggttatc aattgaaaac aggagctaca ttgacttcat
    22801 gctactcctg tttatttaga tttcctgtat cctttaattg acttacaaat atagaaactt
    22861 ttacatcaag ttcgaattga gccctcgatt caatcgatta tctacaggct gccataatca
    22921 tgtatcacaa ctcgattgtt tctgattcct ttgtcattaa gatcacacca tcacctaatg
    22981 gcaagattgt cgatgtcaaa tcgggatgat ccatcacaac acgaagaaat tcatttaatt
    23041 tacgatggat agctcgtttg ccacggggaa tctcttcatc tgggagcaag atcgttcctc
    23101 cttggaagat gtcatctacc atcaaaacgc caccttttct taacaaacgt aagcattcag
    23161 gtaaaaattc aatatatttt gattttgcac tatccataaa aataaagtca tacggaccag
    23221 acaactccgg caagatatct gctgcttgac cttcaagcaa agtcacttta tctgtcagtc
    23281 ctagacgttc atagtttttt ttagctttct cgatcatgac atcgaaacga tcaattgtcg
    23341 taacatgagc gttttctccg attgtcgtgg ccatgacact tgatgaaaaa ccgatagctg
    23401 ctccgatctc caagattcgt tcaggctgaa gctgtccaag taaaaatttc aaaaatacaa
    23461 ctgtttcatg gggaataact ggtacttctg ctacatgagc ttcttcttcc agtttgccta
    23521 gctcacccgg taattttttt tgctgggtac gcataaaatc caatagttct tctttgacta
    23581 caggacgatg catcatttca ttgcgcatac tactcctcct cttcacacat tatacgaaca
    23641 actgactaaa tctacaagct gcatgagtgt tttctcgtgt ttcatgttca gaagagtcgt
    23701 tggatctctg gaatacattt atgtttttaa cattcttaca aaaatttctg tgttcgtctc
    23761 cgcttctttt tcaactctac aaaggtattc catgctgtga ttcctgttag gcaaaaagca
    23821 atcgtaaaga ataacccctg aatccatgca aatgccgttt ttgaaaggac tagtccaata
    23881 tatggaatct gaaagatggg actaccgata atatttctct tttttactac tccaggctca
    23941 atataagtct ttgtatcagc ttttgtataa aaatcttcgt ggttgttttc aacggctatt
    24001 acgcgacgcg tcgagatttt ttttcctgaa ttttcataat atgtaataat atctccgact
    24061 aaaatctcat ttgtattttt cggtcgtaca tatatcagat ttccttgtgc ataatatgga
    24121 ctcatttctt tgtcctcaat aatataagga gtcaaaccaa aaattcgggg aaccaagacg
    24181 acgagaagaa taagaattaa aaatataatc aacgttatag aacttataaa agttattttt
    24241 ttgtaatcag gtatcgtttt caacttcttt ctttattcaa tccttcttaa tcatataata
    24301 acgataaatt ttttcaaatt atagctgtaa aaatgtttcc ctttaataat tatatgtttc
    24361 taattctatt tcctcatctt tatctcattt tttattataa ataattgacg t
//
LOCUS       DAZKAO010000033        24088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000033 DAZKAO010000000
VERSION     DAZKAO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.033"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..3437
                     /locus_tag="ACG8H8_001084"
     CDS             159..3437
                     /locus_tag="ACG8H8_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP8986171.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3978..5885)
                     /locus_tag="ACG8H8_001085"
     CDS             complement(3978..5885)
                     /locus_tag="ACG8H8_001085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP8986172.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6015..6890
                     /locus_tag="ACG8H8_001086"
     CDS             6015..6890
                     /locus_tag="ACG8H8_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP8986173.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6970..7944
                     /locus_tag="ACG8H8_001087"
     CDS             6970..7944
                     /locus_tag="ACG8H8_001087"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP8986174.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8124..9269
                     /locus_tag="ACG8H8_001088"
     CDS             8124..9269
                     /locus_tag="ACG8H8_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP8986175.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9406..9750
                     /locus_tag="ACG8H8_001089"
     CDS             9406..9750
                     /locus_tag="ACG8H8_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP8986176.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10021..11307
                     /gene="efmA"
                     /locus_tag="ACG8H8_001090"
     CDS             10021..11307
                     /gene="efmA"
                     /locus_tag="ACG8H8_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP8986177.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11445..12044)
                     /locus_tag="ACG8H8_001091"
     CDS             complement(11445..12044)
                     /locus_tag="ACG8H8_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8986178.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12448..13569
                     /gene="mnmA"
                     /locus_tag="ACG8H8_001092"
     CDS             12448..13569
                     /gene="mnmA"
                     /locus_tag="ACG8H8_001092"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP8986179.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13815..15236
                     /gene="arcD"
                     /locus_tag="ACG8H8_001093"
     CDS             13815..15236
                     /gene="arcD"
                     /locus_tag="ACG8H8_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP8986180.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15250..16368
                     /locus_tag="ACG8H8_001094"
     CDS             15250..16368
                     /locus_tag="ACG8H8_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP8986181.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16428..17720)
                     /locus_tag="ACG8H8_001095"
     CDS             complement(16428..17720)
                     /locus_tag="ACG8H8_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP8986182.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17870..18430
                     /locus_tag="ACG8H8_001096"
     CDS             17870..18430
                     /locus_tag="ACG8H8_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8986183.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18559..18936
                     /locus_tag="ACG8H8_001097"
     CDS             18559..18936
                     /locus_tag="ACG8H8_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP8986184.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18869..20941
                     /locus_tag="ACG8H8_001098"
     CDS             18869..20941
                     /locus_tag="ACG8H8_001098"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP8986185.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21152..22099)
                     /locus_tag="ACG8H8_001099"
     CDS             complement(21152..22099)
                     /locus_tag="ACG8H8_001099"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8986186.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22345..22809
                     /locus_tag="ACG8H8_001100"
     CDS             22345..22809
                     /locus_tag="ACG8H8_001100"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP8986187.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22871..23914)
                     /locus_tag="ACG8H8_001101"
     CDS             complement(22871..23914)
                     /locus_tag="ACG8H8_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP8986188.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7828 a         4413 c         5065 g         6782 t
ORIGIN      
        1 tttttacgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3601 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3661 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3721 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3781 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3841 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3901 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3961 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     4021 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     4081 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4141 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4201 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4261 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4321 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4381 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4441 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4501 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4561 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4621 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4681 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4741 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4801 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4861 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4921 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4981 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     5041 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     5101 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5161 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5221 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5281 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5341 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5401 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5461 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5521 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5581 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5641 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5701 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5761 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5821 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5881 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5941 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     6001 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     6061 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6121 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6181 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6241 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6301 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6361 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6421 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6481 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6541 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6601 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6661 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6721 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6781 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6841 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6901 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6961 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     7021 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     7081 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7141 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7201 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7261 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7321 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7381 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7441 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7501 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7561 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7621 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7681 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7741 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7801 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7861 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7921 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7981 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     8041 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     8101 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8161 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8221 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8281 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8341 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8401 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8461 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8521 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8581 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8641 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8701 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8761 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8821 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8881 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8941 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     9001 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     9061 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9121 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9181 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9241 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9301 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9361 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9421 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9481 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9541 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9601 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9661 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9721 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9781 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9841 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9901 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9961 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
    10021 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
    10081 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10141 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10201 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10261 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10321 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10381 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10441 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10501 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10561 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10621 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10681 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10741 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10801 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10861 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10921 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10981 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    11041 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    11101 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11161 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11221 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11281 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11341 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11401 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11461 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11521 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11581 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11641 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11701 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11761 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11821 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11881 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11941 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    12001 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    12061 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12121 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12181 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12241 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12301 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12361 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12421 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12481 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12541 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12601 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12661 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12721 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12781 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12841 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12901 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12961 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    13021 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    13081 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13141 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13201 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13261 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13321 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13381 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13441 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13501 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13561 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13621 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13681 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13741 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13801 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13861 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13921 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13981 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    14041 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    14101 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14161 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14221 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14281 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14341 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14401 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14461 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14521 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14581 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14641 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14701 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14761 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14821 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14881 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14941 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    15001 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    15061 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15121 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15181 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15241 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15301 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15361 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15421 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15481 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15541 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15601 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15661 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15721 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15781 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15841 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15901 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15961 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    16021 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    16081 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16141 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16201 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16261 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16321 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16381 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16441 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16501 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16561 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16621 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16681 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16741 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16801 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16861 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16921 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16981 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    17041 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    17101 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17161 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17221 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17281 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17341 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17401 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17461 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17521 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17581 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17641 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17701 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17761 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17821 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17881 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17941 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    18001 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    18061 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18121 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18181 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18241 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18301 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18361 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18421 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18481 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18541 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18601 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18661 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18721 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18781 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18841 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18901 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18961 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    19021 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    19081 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19141 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19201 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19261 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19321 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19381 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19441 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19501 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19561 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19621 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19681 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19741 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19801 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19861 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19921 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19981 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    20041 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    20101 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20161 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20221 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20281 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20341 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20401 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20461 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20521 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20581 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20641 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20701 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20761 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20821 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20881 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20941 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    21001 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    21061 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21121 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21181 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21241 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21301 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21361 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21421 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21481 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21541 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21601 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21661 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21721 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21781 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21841 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21901 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21961 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    22021 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    22081 caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca
    22141 ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag
    22201 gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt
    22261 taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca
    22321 gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta
    22381 gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca
    22441 gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa
    22501 agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc
    22561 tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt
    22621 gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg
    22681 ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg
    22741 ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg
    22801 caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt
    22861 catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa
    22921 gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt
    22981 ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg
    23041 ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa
    23101 accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt
    23161 caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga
    23221 gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta
    23281 agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt
    23341 tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca
    23401 cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga
    23461 tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca
    23521 tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag
    23581 tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt
    23641 gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa
    23701 attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca
    23761 ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgctga
    23821 aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct
    23881 ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat
    23941 actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg
    24001 tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt
    24061 ctacggaaat aagccgaaat ttcacaaa
//
LOCUS       DAZKAO010000034        23825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000034 DAZKAO010000000
VERSION     DAZKAO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.034"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8H8_001102"
     CDS             complement(53..787)
                     /locus_tag="ACG8H8_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP8986189.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8H8_001103"
     CDS             1114..1524
                     /locus_tag="ACG8H8_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP8986190.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8H8_001104"
     CDS             1517..2218
                     /locus_tag="ACG8H8_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP8986191.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8H8_001105"
     CDS             2218..3804
                     /locus_tag="ACG8H8_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986192.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8H8_001106"
     CDS             3823..4044
                     /locus_tag="ACG8H8_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP8986193.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8H8_001107"
     CDS             4041..4802
                     /locus_tag="ACG8H8_001107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP8986194.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8H8_001108"
     CDS             5182..5691
                     /locus_tag="ACG8H8_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP8986195.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8H8_001109"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8H8_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP8986196.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8H8_001110"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8H8_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP8986197.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8H8_001111"
     CDS             7318..8169
                     /locus_tag="ACG8H8_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP8986198.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8H8_001112"
     CDS             8197..8832
                     /locus_tag="ACG8H8_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP8986199.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8H8_001113"
     CDS             8852..9625
                     /locus_tag="ACG8H8_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8986200.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8H8_001114"
     CDS             10033..10830
                     /locus_tag="ACG8H8_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP8986201.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8H8_001115"
     CDS             10808..11221
                     /locus_tag="ACG8H8_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8986202.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8H8_001116"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8H8_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP8986203.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8H8_001117"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8H8_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP8986204.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8H8_001118"
     CDS             15998..16558
                     /locus_tag="ACG8H8_001118"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP8986205.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8H8_001119"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8H8_001119"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP8986206.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8H8_001120"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8H8_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP8986207.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8H8_001121"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8H8_001121"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP8986208.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8H8_001122"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8H8_001122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP8986209.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8H8_001123"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8H8_001123"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP8986210.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8H8_001124"
     CDS             21905..22483
                     /locus_tag="ACG8H8_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8986211.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8H8_001125"
     CDS             22535..23017
                     /locus_tag="ACG8H8_001125"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP8986212.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8H8_001126"
     CDS             23134..23508
                     /locus_tag="ACG8H8_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986213.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6643 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaag
//
LOCUS       DAZKAO010000035        23447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000035 DAZKAO010000000
VERSION     DAZKAO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.035"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..2189)
                     /locus_tag="ACG8H8_001127"
     CDS             complement(45..2189)
                     /locus_tag="ACG8H8_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8986214.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2546..3919)
                     /locus_tag="ACG8H8_001128"
     CDS             complement(2546..3919)
                     /locus_tag="ACG8H8_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8986215.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3919..4314)
                     /gene="rbsD"
                     /locus_tag="ACG8H8_001129"
     CDS             complement(3919..4314)
                     /gene="rbsD"
                     /locus_tag="ACG8H8_001129"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP8986216.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4342..5259)
                     /gene="rbsK"
                     /locus_tag="ACG8H8_001130"
     CDS             complement(4342..5259)
                     /gene="rbsK"
                     /locus_tag="ACG8H8_001130"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP8986217.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5367..6380)
                     /locus_tag="ACG8H8_001131"
     CDS             complement(5367..6380)
                     /locus_tag="ACG8H8_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP8986218.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6461..7060)
                     /locus_tag="ACG8H8_001132"
     CDS             complement(6461..7060)
                     /locus_tag="ACG8H8_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP8986219.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7246..7947
                     /locus_tag="ACG8H8_001133"
     CDS             7246..7947
                     /locus_tag="ACG8H8_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986220.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7957..10668
                     /locus_tag="ACG8H8_001134"
     CDS             7957..10668
                     /locus_tag="ACG8H8_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP8986221.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11119..12747
                     /gene="treC"
                     /locus_tag="ACG8H8_001135"
     CDS             11119..12747
                     /gene="treC"
                     /locus_tag="ACG8H8_001135"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP8986222.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12807..13988)
                     /locus_tag="ACG8H8_001136"
     CDS             complement(12807..13988)
                     /locus_tag="ACG8H8_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8986223.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14240..15763
                     /locus_tag="ACG8H8_001137"
     CDS             14240..15763
                     /locus_tag="ACG8H8_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP8986224.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15832..16560)
                     /locus_tag="ACG8H8_001138"
     CDS             complement(15832..16560)
                     /locus_tag="ACG8H8_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8986225.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16573..17853)
                     /locus_tag="ACG8H8_001139"
     CDS             complement(16573..17853)
                     /locus_tag="ACG8H8_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP8986226.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17994..18596)
                     /locus_tag="ACG8H8_001140"
     CDS             complement(17994..18596)
                     /locus_tag="ACG8H8_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP8986227.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18841..19047
                     /locus_tag="ACG8H8_001141"
     CDS             18841..19047
                     /locus_tag="ACG8H8_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986228.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19359..20078
                     /locus_tag="ACG8H8_001142"
     CDS             19359..20078
                     /locus_tag="ACG8H8_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8986229.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20159..20842)
                     /locus_tag="ACG8H8_001143"
     CDS             complement(20159..20842)
                     /locus_tag="ACG8H8_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP8986230.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21060..23246
                     /locus_tag="ACG8H8_001144"
     CDS             21060..23246
                     /locus_tag="ACG8H8_001144"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP8986231.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7800 a         4222 c         4595 g         6830 t
ORIGIN      
        1 taaaaaggga gtgtgacagt gtcacatccc ctttttaatt tattctattt ctttaaaggc
       61 agatcaagcc agaatgattt gtctaaactt aacaaggtta gtacaggaac agttgcagct
      121 ttaactgtac gagtagttgt ttctggtatc tgtcttgccg taaaattgtt ccacaaatcc
      181 cagattgaag cgacacggaa gatggaagaa gcgttgttga cgcgttttat cgttgaaaat
      241 tctaatcctg tgattggcac gccatctgtt gtccatttga ttggcacatc gaatcgctta
      301 gacatattga ggatttcagc gtttgcttct tcagttgttg ctgcttcttt tttcggttca
      361 tcatattttg tcaggtcgta cgcttcgatg atcgcgttca atttttgtga atattggcga
      421 tctgtagcat aacgtccttc caaatatttg gttgcttctt tgtagtcttt tgtttccgac
      481 ttccaagttg gtttgtagaa atcaggattg ccagtcagtc catttttgat caagtcggca
      541 tagtcttcca aactttcctt gtaagaagga tatttacgga attgtgcgcg gatttggtag
      601 ctttgtccgg aactatcttg ttcaagtgta ttgaaggtca cgctgcttcc tttatatgat
      661 cctttgatac caaaaaggtt atgatttggt tcgcttgata ataagctgtt tccagatcca
      721 ctttcaagga tagcttgagc catcatgact gatgcgtaga ttccttcttt ttgcccgatt
      781 tcctgtgcat catctgcaat ctttttcaag aactgatcag ttgtttggtt ttgactgaaa
      841 tggaacgagc catctaatcc atctaaaata gatgagccgc ctgtcagcga acctgaagca
      901 gcaggtaaac ctgctaagtt tactcggaca gcaaaatcag gcgtgaaatc tgaaatagaa
      961 gttactcgaa ctttatctcc tggttgaggc gcatggataa attgtccgtt cccaatatag
     1021 atacctacat ggtgagtaac accggaagaa ggtccccaga agacgaggtc gccaggttgg
     1081 gcctgactta ctggaatctg cgtacctgca tattgctgtt cgcctgtcca gccaccgata
     1141 taatgaccgg ttgctttcat atatacgtaa taagtcaaac cagaacagtc aaaagtattt
     1201 ggaccttttg ctccccatac atatggtttt ccaagatgct tgaatgcttc tcgaacgatt
     1261 gcttgttgga cattcgttgt atttgtaggc aatacagcat caattggtga ttttacaaca
     1321 aaatcagaag aagcagaaga tggattctct ataccgcttt gatttgctgt atttggacga
     1381 gatgaagcat tgttcgttcc gccattcgat gaaccattgt ttggatggtt tggttctctc
     1441 tgagtatttc cattgttatt cgatccagat gggtttgttt gattcgaaga attcccgctg
     1501 ttcgaattat tgttattatt gtttgaatta tttgaaccat tattattgtt gttattaccc
     1561 acatctggtt tgtttggcgt ttccggatct ggtgtactag ggacttcttc actaggatca
     1621 gttggtttct ctgttgaagt atccggtcca gctttaaatt ctgcagtaat agataaattg
     1681 ttcgtaccta ctgtaaaggt ctttgcatca taatttactt ctttggcacc attaacagcg
     1741 actgaagcaa tttgttcacc ttcagctggc gtaatagtgt aagttacctt cgtttccgct
     1801 aataaacctt ctaccgttcc actttccgta atatccaaag gttgctgttc aggggaatcc
     1861 gctgtcgaag cagtcagttg gatcttacca atcaaagaag gagcaattgt aagggaatat
     1921 gtctcggctg gcttttcatt ttctgatgga tcagccggat caatttcagt tggctcggtc
     1981 gatcctgttg ttgagttagt tgttccagtt gggtcttctg taaaagaatc cgttgtttca
     2041 ctagtagctg agctggatgt agtcgtatct gttgtgactg tttgatcagc tatttgatta
     2101 tctacggcaa atacggtatt ggtcgacaaa acagacggag caatcagcca agtagcagct
     2161 agcaaacgta tcgttttttt atttttcata ataaactagt ccttttcctt tcattgattt
     2221 tctgtcttat tcagcattca ttagacattt tttggaacag ataatcgtgt cgaaaagctt
     2281 tttcttttca taaatctgtc tgtgtattct aaataaaaca atttgtaaat aagcaacgag
     2341 cattcattcg tttttctttg acgttcattt tattgcatag accacctttg tttggggata
     2401 aacaagtggg gataaaaatg gttatgcata tgtaaattat accaaagttt ggaaagtatc
     2461 acaaaaattt ataaaaataa tgatcctaaa aagaaaaaga ctgaaatatg tgataaacat
     2521 ctcatacttc agtctttttc attaactatt cataagacat taggtcttca tcaaacccgc
     2581 taacgagagt agtgatcatt gctacgacta gactaacagc cagtcctgct aagtaccaga
     2641 aaaaagtacc ggaaatgaat gcaggcaatg tggtcagact accaaataca aaagcgatcg
     2701 ctttcacttg ggcaaatccc attacagctc caccagcagc tgcaccaatg caagcagcaa
     2761 caaatggttt tttcaatggg atcgcaattc cgtaaacagt tggttcggtc actcccgcaa
     2821 gtaaaatcga agcaaaagca gaaccggaga tcgaacgggt ttttgaacgt ttacttcgca
     2881 gaaatacacc taaagctgca ccagccattc caaaattggc tgctcctgtc aacggaacga
     2941 ttcggtcgaa cccataagca gagatattac tgatcatgac agggtttaca gcccattgga
     3001 taccaaatat taccaaaata ttccaaccac cagcgatgat tgcgccactg atcggtccgt
     3061 taatatccat catccaagta attgcggcag ctaaaccttc gccaaggtaa acacccgcag
     3121 gcccgaatac cattaacgtc aaaggaaccg tgataactaa tgaaagtaaa ggtacaaata
     3181 ctaattgcat attttcgcga atatattttt tgaggaattt ttcaacaaaa gaataaaaga
     3241 aaatacctaa aatcgccggc atgacagtag acgtatactg catcagtact acaggaatcc
     3301 ccagaaaatc agtagctgct ttgccactgt ctgaaagtaa agaggtcaga ttcggttcaa
     3361 tcagtgctgc agcaatgaca gccgcaatat atccattcac tttaaacgtt ttggcagcag
     3421 aaaaagcaag aaagattggc agaaaataaa agacggcatt cgacgcagca gacaaaatat
     3481 gataagttcc gcttgtttca ctaagccagc caatcgtagt cgctaaaaca accagacctt
     3541 ttaatatacc agaaccggca aacagcggaa tgatcggcgt aaagatttcc gatatcaaac
     3601 caaatagttt ttcgatcgga tttagctttt ctttcggagc tgaatgatcg atttgacgcg
     3661 tcaattcctt gtaagcagga gccaatccct gatccatgat tatttctgca tatctatcga
     3721 gtaaaatgaa atacttaatg gaagggattt gctgttttaa tttttctgca ttggctactg
     3781 gctgtttttt caatttgata cgaagccgag tcgcacatgg aaggacagaa tcaatattgt
     3841 cttttcctcc caactgttca atcagcatat gaatctgctc ggacagtacc atctgttcag
     3901 gattcattgt cgtttccatt agaagaccac tccactcact aatataatat tggcgtaagg
     3961 tgtcatttcg cctgtacgaa taaatgcatg acagttattc aactcttttt tcatttcttc
     4021 atgtgggata aagctaatcg gtgtatctgg taacaataat ttgatttttt ccaatatttc
     4081 cgggttcatt gtctttattt cttccgccaa ttcaatcgat tgtacctcaa gttcttctaa
     4141 aacgttttct aaaacagata aaaaacttgg aagaccgttt gagacagcca aatctatttt
     4201 ttcagtacca aaggggacag gcatgcctgc atccccgata cttaatttat caaagtgccc
     4261 catttgtgcg attactcgtg aaatatctga attgattact tttccttttt tcatgtttgt
     4321 ttcttcctct cattgttaat tttagactgc tgattgttct aaagcttcgc ggtaagggat
     4381 agaaggttgc gcaccataag actgcacagc aacagaagag gctagatttc cataaaggat
     4441 cgcttctttc aagttactaa ggtctggctg aagtgtgctg cttaatgcgc caataaatgt
     4501 atcacctgcg gctgttgtgt caacggcatc cactttaaaa gcagggacga taccgtgttc
     4561 tttttctgta tgatagaaag cgccggcact gcctagtgta atgattactg ttccaatacc
     4621 taattcatgg agtttttcgg ctgctgcgac caaactgtta tgatctgtca ctctgattcc
     4681 tgtaatgatt tctgtttctg tttcatttgg tataatgata tctgtctttt caagtagttc
     4741 agtaggaatc gtttcacgag ctggtgcagg attcaggatc gttgtttttc ctgcgtcttt
     4801 tgcaatagaa aaagctagga tcgtcgcatc taagctactt tcaaactgag caataataaa
     4861 gtcactgttt gtaatcagtt gcttgttttt taggacttct tgttcatgga aagcattatt
     4921 tgcccctgca tggatcaaga tactattttc tcctgctgcg tctaccataa tcattgcttg
     4981 tccggttgtg gcgccttgga tttcagctat cccagatgta tcgatgtttt cttttgtcag
     5041 tagatctaat aactgttgtc cttcgctgtc agccccaacg cctccaataa aagatgtccg
     5101 tgcgcctgaa cgtttggctg ccactgcttg gttggcacct ttacctccgc cagcgtgaaa
     5161 aatctcatgc gcatgcatcg tttcgcctgg cttgggcatg ttggttaaac gaagggtagt
     5221 gtccatatta atacttccaa ttactgtaac cgtgttcatt ttacaaacct ccaactatct
     5281 catttatttt gatgggaaaa ttatttttta aaacgtttta gtaaaacgtt ttaatagttc
     5341 gataaaaata tagcacacct tgaaagctat ttcaagggtg cagttgaaaa tcttttttct
     5401 aatcgtacag gtaatctttt ttcttcccat tctttttctg ggaattggat ccgatctaac
     5461 aataacttgg cagatgtttg tcccaattcg aagattggtt gtgcgatcgt tgtcagtttc
     5521 ggcttgatat actcgcacat atctatatta tcatagccaa taatgctgta gtcttcaggg
     5581 atgctttttc cagcttcctc taatccgcga tataatccaa aagctaattc atcatttaaa
     5641 gcaaacacac cagttgcagc agaatcaaga agttctgctg tgatctggta gccaccttgt
     5701 tttgagaact gtgtaggtaa gaggatcagt tggtcgtgag ggatttggta aacatcaaga
     5761 gcagatttaa acccctcaat ccgagcgtgg acattttccg gtggattttc aggatagaca
     5821 agggcgatcg tttgatgtcc tagactaagc aagtgcatgc ccgctaggta accaccgcga
     5881 aaatcgtctg ttcgtacggc atcactaaat ccttcagatt ttttctgatc caaaacaatg
     5941 aagggccggc cttgtttttt caaattttcg ttgattgcat ctgtagagac agcagaagtc
     6001 gcgataatga atccatccac acctcgacgt gtgagctcgg ctagatattc gatttctttt
     6061 tgatgatcag aatcagcatt acataatatc gtgacgaaat tctgcttata caagatatcc
     6121 tcaattcctc gcatcagtgt actgaagaat ggattcgtga tatcaggaac gagtacacca
     6181 attgttttcg tttccttggt aatcatttgt ctagcaaaat agtcaggctg atagcctaat
     6241 tcttctttgg cagctaaaac tttctgaaca gttttcggac tgaatttctc cgtatttcca
     6301 tttaatatca aggagacagt tgtgattgaa acgccggaaa gtttagcgac ttctttgatt
     6361 gtgatctttt ttttcaccat gttcatactc ctatctaaat cctgtggtta ttttcccatg
     6421 aaatcaaaaa gaagtaaatg aaaatcacgt gtgccagtac ttatttgctt tgttggatag
     6481 cttcgattat ttgtttcgtt gagcgtactc gtgcagtttt cggaaatatc ttttttatga
     6541 acaattgatg ggattctttg tcacgatcac tgcaagcatc ttcagcaagc acgatatggt
     6601 acccgtgttg aaaggcatct aatgcagtag catatacacc gtttgttgtc gctacaccgc
     6661 ttaaaatgat agtttcgatt ccacgccggc gaagttgaag atcaagagaa gtaccaaaaa
     6721 aagcacttgg attgtatttt gtgatagtca acatattatc tgtatctttc aaagaatcct
     6781 tcaaaagtaa atcagtgaat tctggaggta cagaagcagt cattccaccc atatcagttt
     6841 gttgactcaa ataagggaaa gtggagatgt ctacgttcac taaaactttc aaggcagcgg
     6901 tacgtttcaa tgttcttact aacaaatcat tgtttgtcag aatagatgct gcagtgtgtg
     6961 gagagagtgt accgttattt acgatctctt tttgcatgtc gatcgcgaca aaagcagttt
     7021 ttgataaatc aaaaatcata ttttccttta aatcattcat gatgttcctt ctttcaaata
     7081 acaatgtttt ttatgtaagt aaatcataaa tcgtttattt gtcaactaaa ttaaaggatt
     7141 cctttcttta gtctagagaa attcagtggt aaaataaata agtgtgagaa aacgcattcg
     7201 atgcatagat atagcgagaa atatcgtgaa aatggaggaa tcagagtgaa gtatgtcgaa
     7261 gtaaaaaatg aatacaaacg atacaaaatg ggagaaacga ccattacagc aaatgacgat
     7321 atttcttttt caattgaaaa aggcgaatta gtgattatac ttggtccaag tggagcagga
     7381 aagtcaacga ttcttaatat tttaggtggg atggatacac ctgatgaagg tcagatcatt
     7441 attgatgaca cagatattgc gcaattttca gataaacagc ttactgctta ccgacgaaca
     7501 gatgtagggt tcgttttcca attctacaat ctcgttccta atctaacagc aaaagaaaat
     7561 gtggaattgg caacggaagt ttctcctgat gcgcttgatc ctgtagaggt attgaggcaa
     7621 gtgggcctag ctcatcgatt gaataacttt ccgtcccaat tatcaggagg cgaacagcag
     7681 agggtttcaa ttgctcgtgc actagctaaa aatcccaaac ttcttttatg tgatgaaccg
     7741 acaggcgcgc tggattttga aacgggcaag caagtattga aattattgca aaacgctagc
     7801 agacaacacg gcaataccgt tttgatcatt acacataatt ctgctttagc gcccatcgct
     7861 gatagagtca ttcacattaa tgatgctaaa gtaaggtctg ttgaattaaa tgatcatcct
     7921 tcatccattg atgagattgt atggtagggg gattttatga aaacaagaac atatcttaaa
     7981 gcaggttttc gcgaaattcg gcaatctaaa ggacgattca ttgcaatcat ccttattgtc
     8041 atgctaggaa cacttttatt tgtaggtgtc aaaactgctg gacctgtcat gcaaaaaacg
     8101 atggatcact atgttggtac agcaggactt tctgatttgc aaatcgtttc tactggtggc
     8161 ttgacacaaa aagatattgc tcaagcagaa aagatcccag atgcaaaagt agaaacagga
     8221 aaacagatct attattccaa tactggcaaa aacgaagtga ttcaaatatt ttcttataac
     8281 aaaaataaca agcagaacaa gctacaatta actgatggaa aactcccgga aaaaccaaac
     8341 cagcttgtat tggatgaaaa agcggaagat gaagggtata aaatcggtga tatttatcag
     8401 attgattcag atgaattgaa agaaaaagag tataccatca cgggctttgt ccgttctcca
     8461 ttgttcatca acaatttgga gagaggatac gcgaacgtcg gaaatggtag cgtcgattat
     8521 tttgtctatt tacctgaatc cgtttttaaa acagatattt attctgtctt atatcttgat
     8581 tttacaaacg taaagaattt gaatacgtac agcaaagctt acaaagataa aatggagcaa
     8641 aatcaagaaa aggcagaaaa gtatttacaa gatcgacccg aagaacgctt agaagagctg
     8701 aaaaaatcgg ccaacgaaga attagaaccg gcacagcaaa aaatagcgga tggcaaagca
     8761 caaacaacgc aagcaagaac acggttagaa gatgctaaaa aacagttagc gcaacagtca
     8821 gcaactatcc agcagcttcc tgaagcgcaa cgaacaatcg ctgttcaaac attatctcaa
     8881 caagaagctc aacttgctga acaagaaagg caattagttg agaaagaacg tgaactggcc
     8941 tctgcagagg aagaattgaa tcgaaatgca gaaaaaattg aaaatttaac taaaccatca
     9001 tacctataca atgaacgttc agaaaatgtc ggttttcaag aatttggtga tctgtctgat
     9061 cgtatcgctg cgattgccaa tgtttttcct gttttctttt tctttattgc cgcattgatc
     9121 acctttacga caatgacacg tatggtagaa gaaaaccgca gggaaatcgg gacattaaag
     9181 gcgttaggtt atacgaaagt tgaaatcgca aaaaaatatg caatctatgc aagtctagcc
     9241 tcagggatag gtattgtttt aggaacgata ttaggaacga atcttttgcc aaggatcatt
     9301 tttgagctgt ctaacgagcg gtatgatatt gggagtgcgc ttatttttta tgactggccg
     9361 ccgattttac aagcagctgc cgcattcttc attgccgcat ttggcgctgc aatgatcgtt
     9421 ttattcaaag atttaagaga acggcctgct gcattgcttc aaccgaaagc accgaagcca
     9481 gggaaacgca tttttcttga atacatcact ccgctgtggt cacgccttag ctttaatcaa
     9541 aaaatcagtt accgaaatct cttccgatat aagtcaagaa tgcttatggc gattatcgga
     9601 attgctggat gtgccgggct gatggttgca ggcgtgggat tgaaagattc tctaagttct
     9661 gtttcagcca aacaatttgg accgatcatt gactatcaag caatcgttac attggattca
     9721 tccacggaag atagtcgtca aaaagcagcg gatatgctgc agaagcaaga aaaagtaaat
     9781 gagatcctag ctattcatac tcaaaccgta gaattgagaa aaaaaggaca tgcttcgcaa
     9841 agtctgacag tgatcgtacc aagtaaaaca gctgatttgg atcgttttat ccatttaact
     9901 gatcttgaag agaacccacg ctccttgcct aaaacaggaa tcgctattac gcaaaaagta
     9961 gcagaactat ttgacttatc tacaggagat catatttcgc tttatgatga agatcaaaaa
    10021 gaatggaaag tcgaagtaaa agaaatcgtg aaaaactatc taggtagctt tatttatatg
    10081 tcccctgaat attatgcttc agttactgat caatcgatga tggataatgc atttttgctg
    10141 cgtacagata agatgacaac aaaagaagaa gaaatgcttt ccgagaaact gcttgccaca
    10201 gacgctgtga caaatacgtc tttcatgtcc aaacaaatcg aaacgcaaga agaatcaatg
    10261 gcgaacttag actcggttgt cttaatcttt gtcgtattat caggactgct ggcttttatc
    10321 gtcttgtata atctaactaa tatcaatatt tccgagcgtg tacgcgaatt atctacaata
    10381 aaagtccttg gattttttga taaagaagtc acgatgtaca ttgttcgaga aaatatcatc
    10441 tttactcttt taggaatcat cggaggattt ggtgtaggat atatattgac tgacttcatc
    10501 ttgcaacaag cgtctatgga gaatgtcatc tttccattag tgatcacatg gtgggcttat
    10561 gtcttatcag caggattaac tatcgtattt acggttattg tgatgattgt tacccatttc
    10621 aaattaaagc atatcgatat gattgatgcg ttgaaatcga atgaataaaa acagaggttg
    10681 tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gcaaaatagt ttttcatatt
    10741 ttgccgtttt ttgagctaat accgaaggac agctgattga acaccatgaa tcagaggttg
    10801 taaaaaagcc gtcctgcatc gagcaaaaag aatactcaaa taaaattagc tcctcatgtt
    10861 ttacatttga gaagactgat tgccgtagat aaaagctttt gaacaccatt tatagaagaa
    10921 acaagaatat tttgctattt taaacaagag gatagacaaa tacatgtctg atctcttgtt
    10981 ttttattggg caacagacct cttagaatgt ataaagataa attttacaaa cgctttcggt
    11041 tcagttataa taataaatga taacttgtat atataagttg tggtaggtaa taaatcagtt
    11101 gaatggaggt aaatatccat gacatttaaa gacaaagtca tttaccaaat ctatcccaaa
    11161 tcttacaaag acagtaatgg tgatggaatt ggagatttaa aaggaatcaa agaaaaactc
    11221 ccgtatctga atgatctagg catcgacatg atttggctaa accctattta tccaagccct
    11281 caaaaagaca acggttatga tatctctgat tacacagcca tcgatcctat ctttggtacg
    11341 atggaggaat tcgaacagct aatagccgaa gcaaagaaat accggataga aatcatgctc
    11401 gatatggtgc tgaatcacgt ttcgacagaa catgaatggt tcaaaaaagc actagcaggg
    11461 gaccagcatt atcaggactt cttcatttta agagaagagc caacggattg ggtatcaaaa
    11521 tttggtggaa gtgcttggtc gccatttgga gatacgggga aatattattt gcacttatat
    11581 gatcggacac aagctgactt gaactggcgg aaccaagcag taagagagga actattcaaa
    11641 gttgttcgtt tttggatgga aaaaggggta aaaggattcc gtttcgatgt tatcaatgtt
    11701 atcggtaaag acgaacaatt gaaaaataat aaagaaaatg atggcaaacc tgaatatacg
    11761 gacaaaccaa tcacccatac gtatctaaaa gaattgaacc aagcaacgtt tgggacggat
    11821 tccactatca tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg tatcttgtat
    11881 acagaaccta gtagagaaga attatcaatg gtctttaatt tccatcattt gaaagtcgat
    11941 tatgaaaatg ggaataaatg gtctactcct ccatttgatt ttgcagaatt aaagcgacta
    12001 tttcatacat ggggagaaaa gatgagtgag ggtaacggct ggaatgctct attttttaat
    12061 aatcatgatc agccacgtgc gctcaatcga tttgtagcta tcgatcagta tcgaaaacaa
    12121 ggagcagaga tgctagcaac catgatccat ttgaaccgtg ggacgcccta tatttatatg
    12181 ggagaagaaa tcggcatgat cgacccagac tattcatcga tttccgacta tattgacgta
    12241 gaaagtttga atgcttataa gttactcctt gaacaaggat tcacagaaaa agaagcgttt
    12301 cgtcggatca aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg gtcagacaca
    12361 gaacaagcag gatttactac agggaaacct tggttaaaag ttggaggcaa gctagaagag
    12421 attaatgtag aaaaagaact atcttctgaa gattctattt tgtcctatta caagaaattg
    12481 attcggctga gaaaaacata tccaatcgtt gcacaaggag attatcatgc ttatgaagca
    12541 aatcatccgg aagtttatgg ctttcttcgc caatttgaag gacagcagct gcttgtatta
    12601 acaaatttct atgataaaga tacaaggatc gccatcccag aaggattcac agaagccaat
    12661 attcttatcg ataattattc cgatactgtt cttcatcaaa gtctggagct aaaaccatac
    12721 caggcaattg ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc ctacgagcta
    12781 ttctcgtaag atcgacgttt tgtgttttat ttaaaataat cttcttcaga aaatgaagag
    12841 ttttcatact ctcctttatc gccataaaat ttattgtagc gttgtttgaa tttacggaaa
    12901 ataaaagtaa cgagtaattt gactaacgta tagataggaa taccaaaaat aacgcccaca
    12961 attcccatta gatcacccat gacaagtaaa acgattaaga tcgtaatcgg atggatctgt
    13021 aaacgatcac ccattactcg aggaatcacc aagtcaccgt ggagaagttg tacggcaaac
    13081 cagacaatga caaatttgac agccataaaa gtagagtctt ggaatgcaat gatcaaacct
    13141 ggaaagaagg cgataaacgg cccaatatat ggaatgatac ataacaaccc agcagaaata
    13201 gctaaaacac tagcaaattc taatccgatc agtaagaaac aaacccagta gactgctcca
    13261 aggataaatg aagcaatgat ttgccccttt agaaatgaac ccaattgttg attagcaatc
    13321 tttgtcaaat ttttaaaatc tgtacgaaat gctggtggga caatcgctaa aactgactga
    13381 taaaattttt gaggatctct caaaaggaaa aaagcgacaa ttggaccagt aaataaagta
    13441 ataaaaatag ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc actttcccaa
    13501 taatttccaa taaatccagc tatatcagac gtcacgtcgt caatagaagc gaaaaattga
    13561 ttaaatgaat cggcaaaagg tgttttctcc aaaaattgtc ggagactctc ttgaaaatct
    13621 tgaaacaaat ctggcaaact actgatcaat gtctttgcct gttcttcaag aaaaggaata
    13681 agccatatgc ttccaagccc caataaaaat aaaataatca cataaacgat aatgactgac
    13741 aagttcctcg aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag ataatacaag
    13801 atcagtgcaa acaataatgg gggcaagata gtagcgataa tgattccgac aggcttgaat
    13861 ataaacgaga tctgatggaa aaaataaatg atcagcgcaa gtaagataac tattcctaaa
    13921 acatagtaag aggtttttcc tcctaaaaag cggatcaaac gattcgttgt atctgtttct
    13981 tttttcactc tttttcctcc tttatctata acttcattat agacaaaagc gggtataaaa
    14041 ggaaaatgat agaggttgat tgcaaaaata tcgtcaatca ttcatataaa ctttctcatc
    14101 aattaaaata agaaaacgct tgacagttct tttaaccata tgataaatta aatcctgttg
    14161 ctaaaacgta actattacgg tttgcgaaaa gcgaatcgga acattcaaat gaaagaagat
    14221 gagaagagga gtgtcataaa tgaaacgatt gataggtata ttggccatgc tggttttagc
    14281 aggtatgtta actgcttgcg gggcaagtgg gaaagccgag gacagtaaag aaaaattgag
    14341 cgtgatgacg accttttatc ccatgtatga ttttacaaaa gcaatcgtcg gggatgaagg
    14401 agaagtagag ttattgattc cagcagggac tgattcccac gattatgaac cttctgctaa
    14461 agacatggca aagatccaag atacagatat ctttgtttat aatgatgaaa acatggaaac
    14521 atgggtcccc gcaatccaaa aaacattaca agaaggcaat gtccatacga taaaagcgac
    14581 agaaggtatg cttttacttc cgggaagtga agaaggacat gatcatgacc atgaacacgg
    14641 ggaagaaggc catacacatg agctagaccc acatgtatgg ctagctccaa gtttagcaat
    14701 caagcaggta gcaaacatac gtgatcagct gattgaagct tacccagaaa aacaggaagt
    14761 atggacaaaa aatgcagcag cctataccga gaaacttcaa gcactccatc agctttatca
    14821 agaaaccttt aagcaagcaa aacaaagatc ttttgtcacg caacatgctg cattcaacta
    14881 tctagcgtta gaatatggat tgaatcaagt atcgattgct ggtctttctt ctagtgaaga
    14941 accgtctgcg gcgagaattg ctgaactgaa acactttgtt aaagaacatg gaatcaacta
    15001 tatttatttt gaagaaaacg ccaaagatag tattgccaga acgttagcaa acgaagcagg
    15061 tgtatcttta gaagtattga atcctttgga aggattgacg aatgaacaaa tagaaaatgg
    15121 agaaaactat ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa cgacagagac
    15181 agaaaacccg ctagaagata ctttgacccc caaaaaagaa aagagcgtct acaacggtta
    15241 ttttgaagac gaggatgtac aagatcgctc tctttcagat tgggcaggaa cttggcaaac
    15301 agtagatacg tatgtagaag atgggacttt tgatcctgtt tttgaatata aagaaaaatt
    15361 gaatcaggat aagacagcac aagaatataa agaatattat acgaagggct atcaaacaga
    15421 tattgcttca atcaagatca gtgagaaaga gatggcattt acgtttaaga atggaacaac
    15481 gaaacgttca aattatcgtt atgtagggaa agaaatcttg acctacgagg caggaaaccg
    15541 tggcgtaagg tatctattcg aagcagaaga taaggaaagt ggcgctttta gatatattca
    15601 attcagtgat cacacgattg caccggctga ctccgaacat tttcatttat atgcaggaaa
    15661 tgaaagtcaa gaagtattgc tgaaagaaat ggagaattgg ccaacatttt atccagatac
    15721 aatggatgga cgtgaaattg cacaggaaat gcttttgcac tgataggcaa aaaagaaccg
    15781 aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt tttatgaaat
    15841 caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca acgtgacttc
    15901 tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg atttatgaca
    15961 acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga attgtaattg
    16021 ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag aaaaatattg
    16081 gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga agatatcttt
    16141 ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct cgacattttc
    16201 ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt tgctttttaa
    16261 gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta aaggatctaa
    16321 ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta gttcgatatc
    16381 taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa gttcttccgg
    16441 atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc tatttttgat
    16501 caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa tgggaatcat
    16561 atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct ggttttcaat
    16621 tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga taaacaagcc
    16681 aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca actggtttaa
    16741 cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat tcttcttgag
    16801 tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg cattgtccaa
    16861 gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt cgattggctc
    16921 aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt ttaatgacag
    16981 taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg ccggaaaaag
    17041 gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta atccttctaa
    17101 gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat catgtttgaa
    17161 ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt gcatcctgac
    17221 ttgttgctct aataactgat cagctaattt cttattagag attttccaat aataaaccaa
    17281 gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag ctaagacatc
    17341 aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa ataatatcaa
    17401 tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct ctaacgattt
    17461 ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt gaaaatactg
    17521 caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc cgatgcttgt
    17581 taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa ccaattgttg
    17641 acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa ttagagcagt
    17701 aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca gaatgactcc
    17761 cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa ttgtgtaaat
    17821 caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt gatttttacc
    17881 aaattatagc acaattggcg tattattttt ttaatttttc agggattttg aattcagaac
    17941 tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat tgtttaatga
    18001 ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat aggaagcaac
    18061 gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga agctgagaaa
    18121 aataacgtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa taatggggga
    18181 cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg aagcagtaac
    18241 aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa ttgcagatgt
    18301 ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca aacgaattca
    18361 gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag aaaggtgatt
    18421 cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc tagttttcca
    18481 agattattca ataaaacttc taagacaaat tttacttgga catacaccat atcttcttcc
    18541 gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc tttcatctca
    18601 tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt tgtttcataa
    18661 aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt tttccaagaa
    18721 ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca agtttgtgct
    18781 ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg agggattctt
    18841 atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc tgatttattt
    18901 gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat gatacgtgtc
    18961 atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca gttagttgta
    19021 cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca taaagaggtt
    19081 gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag cttttcatgt
    19141 tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta tttgaaaaaa
    19201 gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt acttttggct
    19261 acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt tctataaatg
    19321 aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt gtgatcattg
    19381 ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc caagatttag
    19441 aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta ttttattcta
    19501 atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc tttttggcaa
    19561 tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac gaagatagta
    19621 tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta aagcagtatg
    19681 aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa aagaaaccag
    19741 atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc aaaaaaagag
    19801 gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat aaagaactca
    19861 atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata gtgatcgatg
    19921 gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta caagaaatca
    19981 agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc tacactacaa
    20041 ataacccgaa agcaaaggga atctttacaa ctaaataata taaaaactaa gcaaattgct
    20101 cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt cattgagatc
    20161 aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga gtgagggcca
    20221 tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga tgatgacaga
    20281 aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag tggaaaaatc
    20341 gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt gctacccctt
    20401 ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta tcaaaagcta
    20461 cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga tgctcatgga
    20521 cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca aacaagcgcc
    20581 agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga agaatcccta
    20641 agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct ccgtcaaatg
    20701 cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca ccatattgga
    20761 tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct attgtacgcc
    20821 cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt tgtcttcctt
    20881 atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa tcatatcgct
    20941 tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca acgaaacttt
    21001 aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa atcaatcagt
    21061 tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag aacaagtata
    21121 aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat gaagcggctt
    21181 atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt gaaggaaaca
    21241 gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa gaacaaaaag
    21301 cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct gggagcggca
    21361 aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa gtagacgaag
    21421 aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa aaacgagtag
    21481 ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt atctttttta
    21541 ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga atacaaggag
    21601 acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg atacgtaaat
    21661 ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg ttagactatt
    21721 ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa aaatatgatt
    21781 ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg ctcacggaac
    21841 tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc caaaatctga
    21901 aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat gattatctat
    21961 tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa cggatgacaa
    22021 aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc tatggattca
    22081 gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc aaaactcttt
    22141 tcttattaac taattatcgt tctcgggcag ctattgtcca aggagcaaat agattgatca
    22201 gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa gaagcaggtg
    22261 ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta atcaaacaaa
    22321 ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta taccgatcag
    22381 cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct tttgtactgg
    22441 aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat ttctatcaaa
    22501 aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc taccagcaat
    22561 tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat caatactttt
    22621 ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc cccaatttaa
    22681 aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca gaccagcatc
    22741 aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct aaggaaatcg
    22801 accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact ttcagaagac
    22861 tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc cgcttgattg
    22921 cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa agtaaaggtc
    22981 ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct tataacggag
    23041 cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagta acgacgatag
    23101 aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt ctgtatctat
    23161 gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc aaagaaaccg
    23221 ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt ggtcagtttc
    23281 aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat ttcagcttat
    23341 tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt gacaagaatt
    23401 ttgtcacacc tcccttattt attttgaaaa tgagaatact attatca
//
LOCUS       DAZKAO010000036        22703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000036 DAZKAO010000000
VERSION     DAZKAO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.036"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..235
                     /locus_tag="ACG8H8_001145"
     CDS             65..235
                     /locus_tag="ACG8H8_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986232.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            455..1162
                     /locus_tag="ACG8H8_001146"
     CDS             455..1162
                     /locus_tag="ACG8H8_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8986233.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1222..1866)
                     /locus_tag="ACG8H8_001147"
     CDS             complement(1222..1866)
                     /locus_tag="ACG8H8_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP8986234.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            1993..2280
                     /locus_tag="ACG8H8_001148"
     CDS             1993..2280
                     /locus_tag="ACG8H8_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986235.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2388..2708
                     /locus_tag="ACG8H8_001149"
     CDS             2388..2708
                     /locus_tag="ACG8H8_001149"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8986236.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2789..4102
                     /locus_tag="ACG8H8_001150"
     CDS             2789..4102
                     /locus_tag="ACG8H8_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP8986237.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4099..4818
                     /locus_tag="ACG8H8_001151"
     CDS             4099..4818
                     /locus_tag="ACG8H8_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP8986238.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4946..5497
                     /gene="raiA"
                     /locus_tag="ACG8H8_001152"
     CDS             4946..5497
                     /gene="raiA"
                     /locus_tag="ACG8H8_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP8986239.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5719..8253
                     /gene="secA"
                     /locus_tag="ACG8H8_001153"
     CDS             5719..8253
                     /gene="secA"
                     /locus_tag="ACG8H8_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP8986240.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            8380..9481
                     /gene="prfB"
                     /locus_tag="ACG8H8_001154"
     CDS             join(8380..8451,8453..9481)
                     /gene="prfB"
                     /locus_tag="ACG8H8_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP8986241.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9586..10272
                     /gene="ftsE"
                     /locus_tag="ACG8H8_001155"
     CDS             9586..10272
                     /gene="ftsE"
                     /locus_tag="ACG8H8_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP8986242.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10265..11149
                     /gene="ftsX"
                     /locus_tag="ACG8H8_001156"
     CDS             10265..11149
                     /gene="ftsX"
                     /locus_tag="ACG8H8_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP8986243.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            11301..12749
                     /gene="cls"
                     /locus_tag="ACG8H8_001157"
     CDS             11301..12749
                     /gene="cls"
                     /locus_tag="ACG8H8_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP8986244.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            13086..13943
                     /locus_tag="ACG8H8_001158"
     CDS             13086..13943
                     /locus_tag="ACG8H8_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP8986245.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14058..14978
                     /gene="pstC"
                     /locus_tag="ACG8H8_001159"
     CDS             14058..14978
                     /gene="pstC"
                     /locus_tag="ACG8H8_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP8986246.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14978..15862
                     /gene="pstA"
                     /locus_tag="ACG8H8_001160"
     CDS             14978..15862
                     /gene="pstA"
                     /locus_tag="ACG8H8_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP8986247.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15877..16683
                     /gene="pstB"
                     /locus_tag="ACG8H8_001161"
     CDS             15877..16683
                     /gene="pstB"
                     /locus_tag="ACG8H8_001161"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP8986248.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16699..17457
                     /gene="pstB"
                     /locus_tag="ACG8H8_001162"
     CDS             16699..17457
                     /gene="pstB"
                     /locus_tag="ACG8H8_001162"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP8986249.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17470..18147
                     /gene="phoU"
                     /locus_tag="ACG8H8_001163"
     CDS             17470..18147
                     /gene="phoU"
                     /locus_tag="ACG8H8_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP8986250.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18711..19403
                     /locus_tag="ACG8H8_001164"
     CDS             18711..19403
                     /locus_tag="ACG8H8_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP8986251.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            19400..20167
                     /locus_tag="ACG8H8_001165"
     CDS             19400..20167
                     /locus_tag="ACG8H8_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP8986252.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            20267..21835
                     /gene="liaX"
                     /locus_tag="ACG8H8_001166"
     CDS             20267..21835
                     /gene="liaX"
                     /locus_tag="ACG8H8_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP8986253.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22011..22319
                     /locus_tag="ACG8H8_001167"
     CDS             22011..22319
                     /locus_tag="ACG8H8_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP8986254.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22319..22669
                     /locus_tag="ACG8H8_001168"
     CDS             22319..22669
                     /locus_tag="ACG8H8_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP8986255.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7470 a         3937 c         4914 g         6382 t
ORIGIN      
        1 ttccttgaag attctgctga tttaagatag agttaagtta actacaagaa ttggagggat
       61 ccacatgaaa tggaaagaaa aagtagacga agcagctgaa aagttatatg atttggtcaa
      121 atcagaaaaa tacaatattg aagtgaatat tccgaaaaaa ggcggaaaag cggtccaggt
      181 aaaatcaaaa cgcccaacga atcatacaaa aaaatggatg gctaaaaacc gctaataata
      241 acaaaagatc cataagtcgg cattttcatc gattttaggg tcttttgtta ttttctaggt
      301 tgaacatatc taaaaaattg tttctgacaa taggagtagg aagaacgatg tgtgtctaaa
      361 tcctctgttg atggatagaa acctttatct ttttcagttg tgaatcaggt atataccagt
      421 tataatagaa gaaacgaaag gacgggtgta cgttatgtcg aatcaattac ctgtatatat
      481 acagattcat gaccaattaa aaaatgaaat cgaacaaggt atatggaaag tcggagaccg
      541 tcttccttca gaaagagaat tagcagtgaa attcaatgtc agccgtatga cgttgagaca
      601 agcaattcaa aacttggcag atgaaggaat cttagaaaga aagatcggtt cgggtacata
      661 tgttgcgcga gaaaaagtac aagaaaaaat gtctggtgca acaagtttta cagaaattat
      721 ggaatcgcaa aatcgagtac cgtctagtcg gacaatctct tatttcttga cttcgccaag
      781 ctcaagtgaa atggaaaaac taaacttgag taaagatgac acgatcttgc gaatggaacg
      841 tattcgtttc gcagatgatg tccctatttg ttttgaagtt gccagtgtgc cacaaaagct
      901 gatcgatggc tacagcaaag cagagattac tgaatcgttg tatcgcacgc tagaagaaaa
      961 aggcgggaaa aagatcggtc cagcgaatca aacgattact gctgtgttgg cttctgagaa
     1021 aattgctgac tatttagagg taaaacgagg agatgcagtt cttcgtttgc gccagatttc
     1081 ttattttgaa gacggtacgc cgtttgagta tgtgcgtacg caatacgtag gaaatcgttt
     1141 tgagttttat ttggaaaaat aaaaaacagg tgatccctga ttccttactt agaaataaaa
     1201 gagaatcaga atcacctgtt tttatatatg atcagttacc agttgctcgt gataagaaac
     1261 aggaccctct tcaaattgga cttgtccgtt cagcagctca atgatggatt ctttaaagtg
     1321 gtcagcactt ttttcatcta cagcacagat gacgctaact tgatcagtat attggatttc
     1381 ttttataaaa aaatgttgga gttcaagata attctgtaat tttcctagtt gcggatacgt
     1441 gatcatcaca gagatctcct gttgcaaggt accttctacg attccaatgt cagacaatgc
     1501 ttgagagaca gcatgagtgt atgctcgtat taatccgcct gtgccgagtt tcgttcctcc
     1561 aaaatatcga gtgactactg cagctacatt gatcagttga ttttttttga ggacttccaa
     1621 catcggtacg ccagccgttc cacttggttc tccatcatcg ctgcttcgct ggatttcatt
     1681 tttttctcca atgatgaagg cgctgcagtt atggttcgct ttccaatgtt ctttttttat
     1741 ttcttgaatg aaactttttg cttggtcctc tgattctatt cgtctaagag aacagataaa
     1801 gcgtgatttt ttgatctcaa tctcactttg tccattttct tttattgtac gatagcgctc
     1861 gatcatcttg tcacctcttt ttttcatttt ctttattcag tataaataaa gttctaggtt
     1921 gtttcaacac tgtaacggtt gcgcaatcgc tgtagtatgc gataataaag acaaaggtag
     1981 gtggatctgg ggatgcagga acgaatgaaa aagtacgatg caattacgca ttatttgaag
     2041 aacaatggcg gctctcaagt aacgttgacc tttacccaat ttgacgaact gctgtttccc
     2101 agtaacggac ttcccaaaac agcaagggaa agtacagatt ggtgggcaaa tgattataaa
     2161 caccctgaaa aaggtgccta tggctggata aatgcaggct atgaagtagt tgtcatcaat
     2221 ttggacaaag aatatgtcgt gttcaataaa ttagtgaaat ctagttggct atttgattaa
     2281 ataaaccgcg tttagaaaac gttgacaaat ttctttcttt ttatataata gagtaacaga
     2341 cgcgaagaaa acgtttgcgt agtttgtttt aaaaaggaga gaaaagtatg gctaaaaaaa
     2401 caatcatgct tgtatgttcg gctggtatga gcacaagttt actagtaaca aaaatgcaaa
     2461 aagctgctga agcaaaagga atcgactcgg atattttcgc tgtgtccgct tctgacgcag
     2521 ataacaatct tgcaaataaa gatgtagatg tactgttatt aggacctcaa gttcgcttta
     2581 tgaaagctga ttttgaaaaa cgtctggaac caaaaggcat tcctttagac gtcatcaata
     2641 tggctgatta tggtatgatg aatggcgaaa aagtgttaga tcaagcaatc acgctcatag
     2701 gtgaataaat agaacagtat aactatcgaa caagcgatcc aaaaaataga tcgcttgttt
     2761 tttcgtatat ttttttaggg cgaataatat gaatcatttt tggggtagaa ggcttctgac
     2821 gagagatatt aaagcaatag actgtgagga ggtaaattta ccaaattatg agaagatcgc
     2881 agcagtggaa cgtttgggca actggattct ttgtcatcgc tgtggaatga ggacgtccgt
     2941 cattgatgga cagcttgcag attacggtta tttttgtaca cattgtctat atttagggcg
     3001 atgcgacact caacaagatt tatatttgtt tgatcagcct gaagcaaaac ctcgagaagt
     3061 cgtctttttt tggacaggac agcttactga aaaacaaacg gaaatcgcaa agagaatact
     3121 ttgccattca gaaaaaagac atcatcttat ttgggctgta acaggtgcag ggaaaacaga
     3181 gatgctttat cctattttag tggaaacttt aaaagctgga ggaagagtag cgatttgtac
     3241 accgcgagtc gatgtttgta atgaattgtt tttacgctat cgttatgttt ttccagaaga
     3301 aaaaatcacg ttgctccatg ggaatacagc aacggcttat acctttactt cttttgttat
     3361 ctgtacgatt catcagctat accgttttta tcggtccttt gatttaatag tgatcgatga
     3421 gatcgatgcg tttccttata acggggatgc aggattagct catgctgtgc aaacagcgat
     3481 taaaccagac ggacgattca tctacttaag tgccacacct gataaaaagt tactaaagga
     3541 aatcaagcag acgtttgaca tccacaaact tgctttgcgc tttcatcggc gtttacttcc
     3601 tgaacccgtc ttggttttca tgaataattg gcgacaaaaa tgcaagaaca agaagaagat
     3661 aaaatctttg gtgcggatca ttcatcaatt aattaaagaa aaccatatcc ttttattttg
     3721 tccaagtatc acgatgatgc atcgtttgaa agccacactt caagaggctc ttccgagata
     3781 tgagatcaca gatgtttctt caagagacaa tcagcgggcg gaaaaagtcc agaaaatgcg
     3841 agaagcaaaa tatgacatac tgttaaccac gatgatcctt gaacgtggag tcacatttga
     3901 aagaatatcc gttatcgttt taggagcgga tcatccggtt tttactaaat cttctttggt
     3961 acaaatcgca ggaagagccg atcgtaaagg gccttatacg aacagtcaag tatacttttt
     4021 ctatgaagaa aaaactgcag ctattcgaaa agcttgtcag gaaatcaaag aaatgaatga
     4081 agaagggaag aagatgctgt gaggtgcagt tgctgcagta aagatatcgt ccaaaattta
     4141 acattcagcg agattattgg tgtttcgaaa tacagattac ctcagacttg ttcagtttgt
     4201 cgcgcacaat ttcatttatt ggaaacaaga ggctgttctg gctgtcaaaa accaatccat
     4261 ttcaatctag agagtacgaa aaagaaagta aagtctattc ccgtttactg cagtgactgt
     4321 caagaatgga agaagaaata tccgacctat gattttcagc accgtgcctt ttttgtgtac
     4381 gatgaaaata tgaaggcctg gctcaatcaa tacaagttta tgggagatat ccgactagca
     4441 ggcacctttg catataaatg gacgcagata aaaaaagaat acccgacata catctattgt
     4501 ccaatacctt tatctgaaaa aaggcgaaca gaaagaggat tcaatcaagt aagcgagatg
     4561 ttgaaagcag caagaattcc ttttcagatg ctactcaaaa gagagaagca tctgctggct
     4621 caagcaaaaa acacgcgaaa ggaaagattg gagatgcctc aaccttttga acttgcagtc
     4681 accttttcta agatacaaaa taaaaaaata ttgattattg atgatgtcta tacaacagga
     4741 cagactcttt tccacgcagc agattgtttg caggtcgcac agccgaaaat catccgaacc
     4801 atttctttag caagatgaat tagatgtgga aatttgatag cgatttaatt tgcaaataac
     4861 ctagagttcg atataataga attaagaaga gggaaagagt ggtccttctc cttcttgatt
     4921 gtggtagacg aaaggggtaa tttttatgtt tagatataat gtacgcggcg aaaatatcga
     4981 agtaactgaa gcgattcgcg actacgttga gaaaaaagta ggcaaactag aacgctattt
     5041 cagtgacgca cccgatgcaa cggcgcatgt gaacttaaaa gtttatacag aaaaaacagc
     5101 aaaagtggaa gtaacgatcc cattacctta tcttgtatta cgtgccgaag aaacttctcc
     5161 agatttatac gcaagtatcg atttggttgt tgacaaattg gaacgccaaa tccgcaaatt
     5221 caaaacaaaa attaatcgta aatcccgtga aacgggcatg aatactgcag atgctactgc
     5281 tttattcaat gaagaggacg aaaacgaaaa tgattctgaa ttagatatcg tacgtacgaa
     5341 acgcctttca ttgaaaccaa tggatagtga agaagcagtc ttacaaatga acatgctggg
     5401 acataacttc tttatttttg aagatgcaga aacaaatggt acaagtatcg tttaccgccg
     5461 taaagatgga aaatacggac tgattgaaac agactgatca gccttactta aataacaacg
     5521 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
     5581 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
     5641 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
     5701 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
     5761 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
     5821 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
     5881 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
     5941 cgtgtattgg ggttatatcc ataccacgta caattaatgg gtgggatcgt gcttcacgat
     6001 gggaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgactgcaac gatgccggtg
     6061 tatctgaatg cgttgactgg cgaaggggtc cacgtagtta ccgtcaatga atatttagca
     6121 acccgtgact cgacggaaat gggcgagctt tataatttct taggattgac agttgggttg
     6181 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgcga tatcacttat
     6241 agcacgaata atgaattagg atttgactat ttgagagaca acatggtcgt ttaccgtcat
     6301 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc gatcttgatt
     6361 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatcaac agctttatat
     6421 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatata
     6481 caatcaaaaa caatcagttt aactgaagca gggatcgaaa aagctgaaga aacattcggt
     6541 ttggacaact tatatgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     6601 agagccaact acatcatgct tttagacatc gattatgttg ttcaagatgg aaaagtattg
     6661 attgttgacc aatttacggg tcggatcatg gatggacgcc gttattcaga tggtcttcat
     6721 caagcgatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac gatggcgacg
     6781 atcactttcc agaactattt ccgtatgtac aagaaacttg cggggatgac tggtacagct
     6841 aagacagaag aagaagagtt cagagagatt tataatattc aagtatacca gattccgaca
     6901 aaccgtccgg tcatccgtga cgatcgacca gatttgcttt acccaacatt acaaagcaag
     6961 ttccatgctg tcgtacaaga tatcaaagat cgctaccgca aaggacagcc tgtcttagtc
     7021 ggaactgttg cagttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     7081 cctcatgaag tactgaatgc gaaaaatcac tttaaagaag cagaaatcat catgaatgcg
     7141 ggtcaaaaag gtgcagtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     7201 ctagggcttg gcgtaagaga acttggtggc ctagcagtta tcgggacaga acgacatgaa
     7261 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     7321 tcacaattct acttgtcttt agaagatgat ttgatgaaac gattcggttc agaacgaatc
     7381 aaagctttct tagatcgaat gaagatcggt gacgaagatg ctgtgatcca aagcaagatg
     7441 ctgacgaaac aagtagaagc tgcacaaaaa cgcgtagaag gaaacaacta cgacacacgt
     7501 aagaacgtct tgcaatacga tgacgtgatg cgtgagcaac gtgaagtaat ctataaacag
     7561 cgtcaagaag tgatcatgga agaaaaaagc ttatctaaac cactgatgaa tatggtcaaa
     7621 cggacaatca gtcgtgtggt agatgcgcat acgcaattag aaaaagaaaa ttggaatttg
     7681 gaaagtatcg tcgattttgc tggcagcacg cttgtccatg aagacagtat ttcccttaca
     7741 gatatcgaag gaaaaacctc tcaagaaatc aaagaatatc taaacgaacg agcacaagaa
     7801 gtattcaata ccaaagcagc tcaattgaac ggaccagagc aactgcttga atttgaaaaa
     7861 gtcgtgatct tacgtgtcgt ggactctaaa tggacagatc atattgatgc aatggaccaa
     7921 ttacgtcagt caatcggctt gcgtgcctat ggtcaaaaca atccattagt cgaataccaa
     7981 acagaaggtt ataagatgtt cgaagatatg gtaggtgcca ttgaatatga agtcactcgt
     8041 atcttcatga aagctgagat ccgtcaaaac gtacaacgtg agcaagtttc tcaaggaaca
     8101 gcttctcact cagaagatgg agatgccaaa gaaaacagta atactagcgc gaaaaaacaa
     8161 ccaatccatg tgaacaaggt tggtagaaat gatccttgtc cttgtggaag cgggaaaaaa
     8221 tataagaact gtcacggaaa agatgcaaac taatttctga cagcaggatt ggtgagacaa
     8281 attctttttt aaagaatttg tctcaccttc tttctgttat ttgacgattc tgttagacta
     8341 gatataataa aaaaagtata gtgaaagaag gaaaaaaaca tggaaatatc cgaaattcga
     8401 aatctcctga cagaaatgca ggaaaaggtc acaagcttca ggaggtctct ttgacttaga
     8461 ccagctggaa gaagacattg cggaagcaga agcaaaaatg gctgaacctg gtttttggga
     8521 tgatagcgaa gctgctcaaa aagtgatcag tgaaaacaat gcaaataaag aaacttatga
     8581 tcaattccat tctcttgctg aagaactgga agaaatcgaa ttgatggtgg agatgttgca
     8641 ggaagaacct gatgcagtaa tgcaagaaga agcagaagca cgtatcaatg cgctagatga
     8701 aaaaatgaat acgtatgaat tatctatgtt gttagatggt ccttatgatc gaaacaacgc
     8761 catcgtcgaa ttgcatccag gggctggcgg aacggagtcc caagactggg gcagcatgct
     8821 tttacgcatg tatacacgct gggcagagca gcatgggtat caagtagaga cactagatta
     8881 tcaagcaggc gatgaagcag gaatcaagag tgtgacccta ctgatcaaag gacacaacgc
     8941 atttggctat ctgaaatcag aaaaaggtgt tcatcgcctt gtacgtatct cgccttttga
     9001 ctcagcaaaa cgtcgtcata cttctttttg ttcggtagat gtgatgccag agcttgatga
     9061 aacgatcgat atagaaatca atccagatga tttgaagatc gacacatatc gggcgagtgg
     9121 tgcaggtggt cagcacatca ataagacaga atccgctgta cgaatcactc acttgcctac
     9181 aggaacagta gtagcgagtc aggcacaacg ttcacaattg aaaaaccggg aacaagcgat
     9241 ggggatgctg aaagctaagc tttaccaatt agaagtggaa aagaaagaac aagaagcagc
     9301 tgcactgaga ggagaacaaa aagaaatcgg ctggggatca cagatccgtt cttatgtttt
     9361 ccatccatat tctatggtaa aagaccatcg gacaaacgta gagacaggaa atgtccaagc
     9421 tgttatggac ggagatcttg atttatttat tgacggctat ttgaaaatgc atctgcaata
     9481 agagcccttt gttaaattga aataaaattg taagattttg caagcaagtt gaaacattgc
     9541 catgctataa tagctaaaga ctgaaaaaga tatggagaga acgccatgat tgaaatgaag
     9601 gatgtaatga agaagtactc aaacggtacc actgctatcc gcaatctctc gattgtcatc
     9661 gaacaaggag agtttgtgta tgttgttggt ccgtctggag ctggtaaatc tacattcatc
     9721 aaattgatgt atcgagaaga aaaagcttca aaaggaactt tgaacgtcgc aggtcatgat
     9781 ctaatagcca tcaaaaaccg cgaagttcct tatctacgca gagaaatcgg cgtggtgttc
     9841 caagactata aattattacc aagaaaaaca gtatatgaaa atgttgcgta cgccatgcaa
     9901 gttatcggca gaaaacctcg ggatatcaaa aaacgagtaa tggaagtgct ggatcttgtt
     9961 ggtttgaaac acaaagtccg agtctttcct agcgaattat ctggtgggga acaacaacgt
    10021 gtttctattg cccgagcaat cgtaaataca cctaaagtac ttatcgcgga tgaaccgaca
    10081 gggaatctag atccggaaaa ctcatgggaa atcatgaaac tattagatcg gatcaatgca
    10141 caaggaacaa ctgtcgtgat ggctacacat aacagtacga tcgtaaatac gattcgacac
    10201 cgagtaattg ctatcgaaaa cggccgcatt attcgagacc aagcggaagg ggaatatgga
    10261 tacgatgatt agaacctttt tccgccattt attagaaagt atcaagagtt tgaaacgtaa
    10321 cggctggatg acagtagctt cggtcagtgc ggtaacgatc acgttgactt tagttggtgt
    10381 gttcatggcg gtcatcatga acgcaaccaa actagcacaa gatatagaag gaaacgtgga
    10441 tgtatctgtg ttcgtagaca ttggtacaaa agacgaagag atgaaaaaat tagaaaaaga
    10501 attaaatgac ttagatcatg tcaaaaaagt cgaattttca agcaaagaac aggaattgaa
    10561 gaaaatccaa aacgaaatgg gcgattcttg gaaattgttt gaaggggata ataacccgtt
    10621 atatgatgta tacgtagtaa gtgctaccga tccttcttat acaaaacaag tagcagaaaa
    10681 agcggcagat ttgaagaatg tttcccgttc tgattacggt ggggcttcct cagatcggat
    10741 tttccaaatt gctggtgcag ttcgaacatg gggattagtt gcggcggcat tgctgctgtt
    10801 tgtagcaatg tttctcattt ctaacacgat tcggatcacg attttatcta gacagcgtga
    10861 aattcaaatc atgcgtttag taggggcaaa aaatggatat atccgctggc catttttcct
    10921 tgaaggtggt tggatcggct taatcggtgc cattcttccg atactgatca ttacatttgg
    10981 ttacgcatgg ttttaccaat tgatcaatcc gtcattatta cgatctcatt attcattgat
    11041 ccatccagaa aatatcgttt ggaaaatcaa tcttttgatg gtcggtattg gtgtgatcat
    11101 tggttcactc ggctcgatca tctctatgag acgtttcttg aaattctagt gtcacaagat
    11161 aaatataatc agaaagttgg acgatttcgt tccagctttc tttttttatc agctaggtgg
    11221 agtaaatggg gcggcttttt tctctttact ttaaaaaaca acttgggtat tcttaagttg
    11281 gtagaaaaag aggtgaagcc gtgaagatta tcgttgacaa tttttttacg atccttttga
    11341 ttatgaatat tttgctttcc tttattatcg tttttcgaga aagaaaggaa actgcgcaaa
    11401 catgggcatg gctgctcgtt ttgatgttca ttccagttgt aggatttatt ttatatattt
    11461 ttttaggaag agggatttcc aaagagaaaa tctttgattt gaagatccaa gggaaaatcg
    11521 gtaagaatct agaaatcgaa gaagataaac aagcattgat gcgagggctt tatcctcatc
    11581 cgcctactgg acaagtagat gtgaaacaat tgatctatat gcttactgta tttgaaagta
    11641 cgctttatac gactaaaaat gaaatcactt tatttacaga tggtcgtgaa aaattcgatg
    11701 cgctgatcga ggatatcaaa caggcagaag accatatcca tttccaatat tatatttacc
    11761 gcagtgatac attaggtgaa gaagttcgag aggcattgac agaggcagca agacgcggag
    11821 taaaagtcag agtccttctt gatgcatggg gttccacaca agtttcttct agtttttttc
    11881 agaatttgaa aaaagcaggt ggagaagtcg catttttctt tcctctattc gttccgtata
    11941 tcaatcctcg aatcaattat cgcaatcatc gtaaaatcgt agtgattgat gggaaaatcg
    12001 gatatacagg tgggttcaat gttggaaacg aatatctagg attggtaaag aaatttggtt
    12061 attggcgaga caatcatctg cgtatctatg gagaagcggt ttacaccttg caaaaccgtt
    12121 ttttgatgga ctggaattct cagcatacaa aagaagtagg atattcaccg gaatttttcc
    12181 cgaccattca ttctacaggt gatatggctg ttcaaatcgt gacaagcgga ccagataccg
    12241 aacatgagca aatcaaaatg acatatttga aaatgatttc gttagccaaa cgggagatat
    12301 tgatccaaac gccgtattac ataccggatg gatcgattca tgaggcattg aagttggctc
    12361 ttctctcagg cgtaaaagtc catatccaaa tacctaacaa accagatcat cttctcgttt
    12421 actgggcaac gtattctttc gctgcagagc tgatcgagta tggggcaaga atcgaaacat
    12481 atgaaaatgg atttatccat gcaaagacga tgattatcga tggcggcatc gtttccgttg
    12541 gttcagctaa tatcgacgtt cgctcattta gattggattt tgaagtcaat acactgatct
    12601 atgatgaacg aatggcttct cgcgtaagaa aagcattctt tgaagattca aagatttcga
    12661 cgcatctcac aaaagaagtg tatgaaaacc gagggatgat gatcaagatg aaagaaggat
    12721 tagcaagact gatttcgccg ctcctttaaa atacaaaaag gacaggagag ccatcaatag
    12781 atggaaatct cgcgtccttt tttctcgggg ccatttctga gagtacgtca gaaatggaga
    12841 aagcgtctcg catgagcgaa attaactttg gggtatctct tgttttttgg ggaaagcaag
    12901 aaataagcta atatatgcta ttaaaagaga cacatttcaa agccagtata gcatagaaac
    12961 ttttaatgag atagcttttt ttgcaattat gagaatttat tttgttattt aatgattttg
    13021 attcgttttc atcaagtaat gaaaatgata gaataaacat actttaggat taaaggagat
    13081 ttctaatgaa aaaactaaaa ttattcttac caataatagg attagctatt cttttatcag
    13141 gctgttcaaa gtggattgat cgaggagaat ccattactgc agttggctct tctgccttac
    13201 agcctttagt agaaacggtt gctgaaacct accaggcaga aaatcctgga aaattcatca
    13261 atgtacaagg gggcggaagc ggaactgggt tgtctcaagt acagtcagga gcagtcgata
    13321 tcggaaattc tgatttgttc gcggaagaaa aatcagggat tgatgcttcg gcattagtcg
    13381 atcatcgagt agcagttgtt ggaatcactc ctattgtgaa caaggatatt ggcgtaaaag
    13441 atatcagtat ggaaaacctg aaaaaattat ttacgggcga gatcaaaaac tggagtgaag
    13501 tcggtgggaa agatcagcca gtagttatat tgaaccgtgc atcaggaagc gggacccgta
    13561 gtacgtttga aaaatgggta ttggacggag aaacagctgt tcaggcacaa gaacaagatt
    13621 caagcggaat ggtgcgccaa atcgttgcgg acacacccgg agcaatcagc tatgttgctt
    13681 tttcttacgt ttccgatgat gtggatacgt tgaacgttaa cggtgtggaa ccaacagatg
    13741 aaaatgtgac aacgaacaaa tggacgattt gggcatatga gcatatgtac acaagagacc
    13801 atccaaaaaa actgacaaaa gaatttttag actatatctt atctgatgaa gtacaagata
    13861 atattgtcgg agagctaggc tatatccctg tttcacatat gaaagttgaa cgtgattggc
    13921 aaggaaacat tgttaaagag taatctttac acaaaattta caaacttagc taaactttta
    13981 catgttatta aaaccaaatt gatgttcaat ttgtacactg agtgagtatg ttaaacctga
    14041 ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt attaacaaaa tcaaaacgtt
    14101 caaaaatgga acagcgcgga aaattcatta gttttttgtg tatcgcgctg atcgtgttgg
    14161 ttgtattatc tattttttat tttgtcgcta gtaaaggatt ggccaccttt tttactgata
    14221 agatcaatgt atttgatttt ctgtttggta cagattggaa tccaagccaa ataggagcaa
    14281 atgggaaacc catggtaggg gcattgccta tgatcacagg ttcatttatt gtgacctttt
    14341 tatctgctat tgttgccaca ccatttgcta ttggagcagc agtgttcatg acggagattt
    14401 ctcctaaaaa cggaacgaag atcttgcaac cggtcatcga gctacttgta ggtattccgt
    14461 ctgtcgttta cggatttatc ggtttatctg ttatcgtgcc ttttgtcaga agtatctttg
    14521 gcgggacagg gttcggtatc ttagctggta catttgtgtt attcgttatg atcttaccga
    14581 ctgtcacaac gatgactgtg gatgcattaa aggcagtgcc gcgacactat cgggaagctt
    14641 cattagcgct aggtgccaca agatggcaaa caatctacaa agtggtgctg cgagcagcag
    14701 tgcccggaat tttaacagca gtcgtttttg gtatggctcg tgcgtttggc gaagccttag
    14761 ctatccaaat ggttattggt aatgcggcat taatgccaac aggacttact actcctgctt
    14821 ctacattgac gtccatcttg acaatgggaa tcggaaatac aatcatggga acagttgaaa
    14881 ataatgtgtt atggtcatta gcgctgatct tgctattgat gtcattattc tttaatatcg
    14941 tgatccgtat gattgggaag aaaggagagc tgaaataatg aacgcaaaac gctcagataa
    15001 aattgctaca ggtgttctat atgtggtttc agggatcatc gtattgatct tagcagcttt
    15061 attactttat atcttagttc gaggaatacc gcacatttca tggtcattct tgactgaacc
    15121 ttccaaagcc taccaagaag gcggtgggat cgggattcaa ttatttaact cgatttattt
    15181 gcttttgatt accatgatca tcagttttcc tatctcttta ggtgcgggta tctacctttc
    15241 ggaatatgcc ggaaaaaact ggctgacaga cgtgatccgt acgtctatcg aaatcttgag
    15301 ctctttgcct tctgttgttg tcggtttatt cggtttcttg gttttcgtta ttcaattcca
    15361 gtatggtttc tcaatcattt ctggtgcatt agctttgacc gtattcaact tgcctctttt
    15421 gactcgaacg gtcgaggaat ctttacaagc ggtccattac acacaacgag aagctggatt
    15481 ggcgttaggt ttgtcacgct gggaaacagt gataaaagtg gttgttccag aagcgacacc
    15541 aggaattttg actggtgtga tcctaagttc aggaagaatt tttggtgaag ctgccgcatt
    15601 gatttataca gctggtcaaa gcgcgccagc attagatttt acgaactgga atccattaag
    15661 tgtttctagt ccgatcagca ttttccgcca agctgaaaca ctggctgtcc atatttggaa
    15721 aatcaacaca gaaggtacga tgcctgatgg tgtttctgtt tcggctggcg cttctgccgt
    15781 gttgatcatt gctgttttac tgttcaactt cggcgctcgt gcgattggca aacgattata
    15841 ccgaaaaatg acttcagcct aaggagaaga agtaaaatga aaaaatataa ttggaatgaa
    15901 acaaatgtca ttaccatgcc aaaagataag gaagttgcat tatatacgaa tgatctgcat
    15961 gtttggtatg gcgacaatga agcaatcaag ggagtggatc tagaattcga aaaaaataag
    16021 atcactgcat tgattggtcc ctctggctgc ggaaaatcca cgtatcttcg ttctttgaat
    16081 cgtatgaatg atgggattgc taatacgaaa attactggaa agattatgta taaagacgtg
    16141 gatatcaatg cgcctcaagt ggatgtttat gaaatgcgta aacggatcgg catggtgttc
    16201 caacgcccca acccttttag caaatcgatt tatgacaata taacttttgc tttgaaacag
    16261 catggtgaaa aaaacaaaaa gaaattagat gaaatcgttg aaacaagctt gaaacaagca
    16321 gctttgtggg atcaagtaaa agatagcttg gataaaagtg ccttggcttt atctggcgga
    16381 caacagcaac gtttgtgtat cgctcgtgca atcgcgatga aacccgatat cttgctgtta
    16441 gacgagccgg caagtgcctt ggatcctatt tcgacaggaa cggttgaaga aacacttgta
    16501 aacttgaaag aagactacac gatcattatc gtgacgcata atatgcaaca agcagctcgt
    16561 atcagcgact atactgcctt tttctatcta ggaaaagtaa tggaatatga caaaacaaac
    16621 aagatattta ccagaccaaa aattcaggca accgaagatt atgtgtccgg tcattttggt
    16681 taagacggag gcctcaacat gacaaaagaa attatctcat ccaaagatct tcatctgtat
    16741 tatggaaaga aagaagcact aaagggaatt gatctaagtt tcaatcaagg tgaaatcaca
    16801 gcgatgatcg gtccttctgg ttgcgggaaa tctacctatc tacgttcgtt gaatcgaatg
    16861 aacgatctga ttccaaatgt aacgatcacc ggaagtgttt tatataaaga caaagacatc
    16921 tatggtccga aaacagatgt tgttgaacta agaaaagaaa tcggcatggt gttccaacag
    16981 ccaaatccat ttcctttttc tatttatgaa aatgtcatct atggattgaa gttaaaaggt
    17041 gaaaaagata aaaaagtcct tgatcaagta gtggaagaaa gtctgaaggc agcgtccgtt
    17101 tgggaggatg tgaaagataa actgcataaa agtgccttgt ctttatccgg tgggcagcag
    17161 cagcgtgtat gtatcgcacg tgttttggca gtgaatcccg aaatcatctt attggacgaa
    17221 ccaacaagtg cacttgaccc tgtttctact gggaaaatcg aaagtatgct gttagaatta
    17281 agagagcggt atacgatgat catcgtgacc cataatatgt ctcaagcttc acgtatttca
    17341 gataagacgg cattcttttt agatggtcac ttgatcgaat acaacgatac gaagaagata
    17401 ttcatgaatc cagaaaaaca agagacagaa gattatattt caggtcgatt tggataagga
    17461 ggaaacggaa tgctacgtac acaatttgaa gaagaattgc tgaatctcca taatcagttc
    17521 tacgaaatgg ggatgatggt gagcagcgct gtgcacaagt cagttcgtgc atacatcaaa
    17581 cacgataaaa tcttagctca agaagttatc gaaaacgata taaatatcaa taatatggaa
    17641 acgcgtttgg agaagaaaag ttttgaaatg attgcattac aacagccagt tacaactgac
    17701 ttgcgtatga ttatcacggt catgaaagct agctctgatt tagaacgtat ggccgatcat
    17761 gcggtgtccg ttgctaaatc cacaatccgc gtaaaaggac agacgagaat accggaaatc
    17821 gaaaaagaga tctccgatat gtccgattac gtgaaaaaaa tggtggacaa cgtcctagtt
    17881 gcgtatgtca aaacagacga aaaagatgcg cgtacaatcg ccaatatgga tcaaagagtc
    17941 aatgactatt tcaatcgaat ctataatgca accatcaaaa acatgcaaga aaatcctgaa
    18001 acagtcatca gtggcacaga ctatttgaat gttgccagct atctagaacg aatcggagac
    18061 tatgtgacaa acatctgtga atggatcgtc tacttggcta cagggaaaat cactgaatta
    18121 aatacgaacc acaatgagat tttctaaact gaagttgtga ttctgctgtt tatttaggaa
    18181 aagaggttgt gaaatcagcg ttttgtcctg agtattagag aaacaaacgg taaaatagtt
    18241 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat
    18301 tctggaaaag ggtttgtgac aaaacatgtc acaaaccctt ttcattattc caatagaaag
    18361 gataaagaat gcaaaaaaag attagcatca tcatcactct attttttatt tgtatacttg
    18421 cttcatggtt tattcagcgt caagctgctc tatatgacgt acctgtcatc gagatcgaaa
    18481 gagtgagaat agaagaggat caacaattga ttacaggaag attgatgaat acggaccaaa
    18541 aaggagaagt gatttcttta acggctcctt atcaaaaaaa tcaaatcgaa acagcaaata
    18601 taacaacaag acaaatctat cttgtccaaa tgcatggaga tgaatggcaa ctagtagcaa
    18661 aaaaaaatga tggctggctt tttttcttta caagtgtctt cattggcacc atgttgctta
    18721 ttggtggaaa acagggcgta tttgctttag gaagtctcat catcaacatc gttttacttt
    18781 tacttttgtt gctcctcttt accaaaacag aaggtatttc tctcttaaat atagcaatct
    18841 tatttgtcgt tatagggata atcgttagta tattggcgtt agacggatgg aatagaagca
    18901 gtataatcaa aatttcggct gcagttacaa gtgttttttt agcatttttt atttgtcttt
    18961 tgacaatgaa tcattggaaa gatcaaggac tgcgctatga agaactgaat tatctgacac
    19021 gaccgtatcg ttctgttttt ctttccagcg tgctgattgg gacagctgga gggacactcg
    19081 ataccgtgat cacagtcatt gcgacattag aagaattgac caagcaacaa ccgaaaatat
    19141 cagcgaaaca gttatggctt tcaggaaggc aagtgggccg agatgtgatt ggttcgatgg
    19201 ctaatatcct tttattcgtc tatatcagcg gagcgattcc atcgcttctg ttatatttag
    19261 gaaatcaatg gtctttcaaa gaaacggtag aacgacattt atcattagaa cttttacgcg
    19321 tcattgctgg cagcttagga atagtattat caatccccat cgcggtcctc ttttttctcg
    19381 ctgtaaggag gttaaaaaaa tgaatgtact tattatactt gttgggattt ttgcaatcag
    19441 tgtgctattt gtcggtggta cgcaaggtat gtatattctt ttaggattat tcattaattt
    19501 aggaatcttt ttcctattat tattcggcta tcatcagaag tggccgatat tggtattgtc
    19561 gattataggg ttcctattga tcgctgtggt tatcttattt tttatcaatg gttataatct
    19621 gaaaatgcga gccgcttttg ccagtatact gatctttttg ttttgtttcc tactattgat
    19681 tcctatcact gattttttag cgatccaagg ctttacttcg atagaactag aagaactatc
    19741 tggcttagat aagacgttag caattgattt tagactactt acgaggtcgt tgcttctgat
    19801 cagtcttagc ggagctgttt tagatgcgag tgtagcgatt tctagtggaa cattcgaagt
    19861 atatcaggca aatccgcaaa tgtcttttaa ccaattgcgc catgctagct ttgccattgc
    19921 taaaaaaata ttggcatcaa cagtgatgac gcttcttttt gcgtttatgg ggagttcttt
    19981 agcgttgatt ctttggttta ttgacttaga catttctttt cagcaaataa tcaatgaaaa
    20041 atcatttgtc ttggaatata cgatggctat tcttaccaca ctggccgcac tacttgtcct
    20101 gcccttaact tgcgtgacta cagcatatct ctttacaaaa aagaaagaac atctcaagat
    20161 ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga
    20221 tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga
    20281 acgtatttta gacttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt
    20341 agaaagcatg gcaactgaaa aagacgaaaa acaaatcaaa aaagaagcag acaaggtaac
    20401 tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gatgaacttg aaaacggtga
    20461 agaaaaaatt tctgagccag ttgatccaaa agaaaaagaa agacaagatc aagaaaactt
    20521 agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagacga
    20581 agtaaatgct caattcgctg acaataaggc agcacgtaat gagaaacagg aagcattgat
    20641 gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca
    20701 aacgttagaa gcagaaatca aagagttaga agcaaccggc gatacactat tagaagaaca
    20761 attgaagctg gaagcagaat taaaagatat ccgcaagaat caatggtctg agaaaaaaga
    20821 aacatttacg gataagtttg aattgccaga tgattggaaa gatcaagcga cagatacgct
    20881 gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa
    20941 aacattccaa actgtttcag aaacagtaaa tgataacatg gaatggaaag atgtcagctt
    21001 acgcgtccct ggaattgcga caacgaaatt tgaacacgaa ttttattatg aagctcctgc
    21061 agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag
    21121 tgatgatgtg aaagtggaag ccaagatcaa actttacgga aaaatgggtg ctgaaccatt
    21181 tgaagcattc tctgaacgga gccagatcga ggtcaatgaa gatcatatct ccttccaaat
    21241 tccaaataag cgtgtgcgtg cagaccttgt tttctacttg cctaaacgag tatacgacca
    21301 tgcagctatc aaacttctaa atggcaacat catgattgag acattggaag ctaaagatat
    21361 ttatacaaaa tctacgaatg ggaacatcat cgtgaaccaa ttaactgcaa cgatgctgga
    21421 agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat
    21481 agaaacagta aacggtacag tgacatttgg tgccacacca gaaaaccttt ctgtgtcttt
    21541 agtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag
    21601 ttcagtgaac gggaatgtca aagttgcttt accagatagc atcggtttgg aaggacacgc
    21661 taaaacaagt cttggaagca ttaacagccg tctgtccaat tacgaagtag ttcgtgagaa
    21721 aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatgatg agatcgcaca
    21781 agtgcagctt tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg
    21841 ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttttaaaat agcttcccct
    21901 attttaaaaa aatgtgcctt attgccgata ggctatctct tgaacaccga ttaagaagaa
    21961 ggttgtcaca acgattttct tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa
    22021 aaaagttgac aaaatccaat aaaaatgttg ttttgacagg tactttagca gggattgggg
    22081 aatatatcgg gattgatccc actgttatcc gtgtgcttta tgtattctta agcttagttg
    22141 cttttggaag tccaatcatt ttatacattt tattagcttt gattatccca tctgcgcgtt
    22201 ctgcacgaac aaactatgga cataacaatg catattatga aaaaaacaat tatcgtgaac
    22261 aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga cgaatggagt gacttttaat
    22321 gtcgtacttt cagcgtattt tggttaatac gttaacattc atatcgttgg ctgttctttt
    22381 tcctgcgatg gtccatgtga gcagtatttg gacggccgtc cttgccagct tcgtattatc
    22441 tgtgttgaac atgtttgtca aaccgatttt gacgatcctg tccctgccct ttacattagt
    22501 cacattggga ttgtttacgt ttgttatcaa tgcagcgatt cttaaaatga cttccttttt
    22561 tgtaggagca gaacatttta gtttttcttc atttggtgca gcgttattcg tagctatctt
    22621 gatgtcactt gtcaatgtaa ttgttagtga acataacagc agaaaataaa aaaagctgtg
    22681 ccgagatctc ggcgcagctt ttt
//
LOCUS       DAZKAO010000037        22578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000037 DAZKAO010000000
VERSION     DAZKAO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.037"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..287)
                     /locus_tag="ACG8H8_001169"
                     /pseudo
     CDS             complement(195..287)
                     /locus_tag="ACG8H8_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(423..1058)
                     /locus_tag="ACG8H8_001170"
     CDS             complement(423..1058)
                     /locus_tag="ACG8H8_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986256.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1570..2712
                     /locus_tag="ACG8H8_001171"
     CDS             1570..2712
                     /locus_tag="ACG8H8_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8986257.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2705..2911
                     /locus_tag="ACG8H8_001172"
     CDS             2705..2911
                     /locus_tag="ACG8H8_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP8986258.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            2990..4183
                     /locus_tag="ACG8H8_001173"
     CDS             2990..4183
                     /locus_tag="ACG8H8_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8986259.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4388..5527
                     /locus_tag="ACG8H8_001174"
     CDS             4388..5527
                     /locus_tag="ACG8H8_001174"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP8986260.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5520..6320
                     /locus_tag="ACG8H8_001175"
     CDS             5520..6320
                     /locus_tag="ACG8H8_001175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8986261.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6320..7147
                     /locus_tag="ACG8H8_001176"
     CDS             6320..7147
                     /locus_tag="ACG8H8_001176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8986262.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7152..7859
                     /locus_tag="ACG8H8_001177"
     CDS             7152..7859
                     /locus_tag="ACG8H8_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8986263.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            7959..8825
                     /gene="rfbA"
                     /locus_tag="ACG8H8_001178"
     CDS             7959..8825
                     /gene="rfbA"
                     /locus_tag="ACG8H8_001178"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP8986264.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            8839..9411
                     /gene="rfbC"
                     /locus_tag="ACG8H8_001179"
     CDS             8839..9411
                     /gene="rfbC"
                     /locus_tag="ACG8H8_001179"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP8986265.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9433..10461
                     /gene="rfbB"
                     /locus_tag="ACG8H8_001180"
     CDS             9433..10461
                     /gene="rfbB"
                     /locus_tag="ACG8H8_001180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP8986266.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10559..11410
                     /gene="rfbD"
                     /locus_tag="ACG8H8_001181"
     CDS             10559..11410
                     /gene="rfbD"
                     /locus_tag="ACG8H8_001181"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP8986267.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11444..13477
                     /locus_tag="ACG8H8_001182"
     CDS             11444..13477
                     /locus_tag="ACG8H8_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986268.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13521..14801
                     /locus_tag="ACG8H8_001183"
     CDS             13521..14801
                     /locus_tag="ACG8H8_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP8986269.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15011..15817
                     /locus_tag="ACG8H8_001184"
     CDS             15011..15817
                     /locus_tag="ACG8H8_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986270.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            15829..17049
                     /locus_tag="ACG8H8_001185"
     CDS             15829..17049
                     /locus_tag="ACG8H8_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986271.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17039..18625
                     /locus_tag="ACG8H8_001186"
     CDS             17039..18625
                     /locus_tag="ACG8H8_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8986272.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18664..20802
                     /locus_tag="ACG8H8_001187"
     CDS             18664..20802
                     /locus_tag="ACG8H8_001187"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8986273.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21170..22153)
                     /locus_tag="ACG8H8_001188"
     CDS             complement(21170..22153)
                     /locus_tag="ACG8H8_001188"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8986274.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7203 a         3884 c         4676 g         6815 t
ORIGIN      
        1 atatcctttt tcacaaaaca tttttgtaga aacatctaaa atgagtttta ctgtttcttc
       61 tggatattta tttcgtgcca taacttacct ccaatacaaa ccgttggtct gattaaagta
      121 tatcttagta ttttatgctt gtcaatataa agacttgcaa ttttataaaa atgcagaatg
      181 caagcctttt tcatttattc tacaatcttc cagttgcttt ccttatgcgg tgtcaattca
      241 tactgtgata cctgcgttgc ctttgcctga tttaccacaa atcagatttt atccctttct
      301 tcctcttgat tgcccacaac agaaaagaaa aagccctgtc aagagctttg ccaccattgg
      361 tggtgctttg cactcttgat cgggcttttt gtttgctgta tcttctcgca agaggaaaaa
      421 agttactcgt cctcatcatc aaaattgtta ttccttaata cttctcgtaa cagttccata
      481 tcttcttttg agttcgggtt tatcattttc acgacttgat aaggtgtctt atacttatgt
      541 ccctctatgc tttccccaaa catatccata ctgtataaat catcatagcg gtctaataat
      601 ttttgaaact tcaaatgctg gataaattct ttgattgggt aaaggtcgga cgcttcaatg
      661 cttttcccgt tgatatagtc tgtaatatat tcccgtaact tttctaactc atattccagc
      721 cattcttcct cgctgtcaaa gaagttatca tagtgtccat gtttcagttc tgcatttaga
      781 cgttcttcgg ctcttaaaaa ctgacagact tccttttcag attgattgac atacttccat
      841 tctcccgaca acaattcatt gggcgtaacg tccagcactt ccattatctt ttccagcgtt
      901 tcataggcag gatagttcaa ccctctttca atcttggaaa ggctctgcat attgataccg
      961 attttgtccg caagttcctg ttgtttcatt cccctaaatt ttcttatggt ctgtatatta
     1021 tttcctaaat ggcttatttt cattgcataa ccctccatat ctcgttatat cttcgctatt
     1081 ataagtctat catgcttaat atgatttgta aaggcaaaaa aagatattga caaataagca
     1141 tataagaatt actatgttct tgtaatcgaa taagcacaat agaatgaaat ataaaaacat
     1201 tcaaaaagaa atacctatct gattaaatcc gtagatatga atatcggttg aaatggaact
     1261 tgttagaaaa ggcaaaacag aacttgctga caaagtattt ctgacacggt ggcggaagcc
     1321 tctcaggctt accgccatga caccttgtgt caaggggaag aaatgctttg tcggctggcg
     1381 agcctgcgag ccttgtctgt gcccccgtta tctaacaggg gggcactatt tcacaaaaga
     1441 caagtcaaaa accatacacc cccaaacctg tgagtagtaa tgagaggttt taagaagata
     1501 gtacatatca cactttttga aaggtggttt acaggtattt caaggctatt gtgggattgg
     1561 aggaatacaa tgaacaattt agaatttgca cttgaaatga agaacaaacg tcttgccagc
     1621 ggtctttcac aaggagaact tgcaagtctt acccatgtat caagatatag tatcaaccgc
     1681 tttgagaacg gaaaagctaa cgccagcaag gaaacacaga acattatcct gcgttgtctg
     1741 aattactata tctgcgataa gcctttttat ctgctcgttg attatctgtc tgtccgtttt
     1801 ccaacgactg acgcattgga agttatccga aaggtgcttg gcatgaaagc agattatttt
     1861 atccattatg actatgggta ttatggctat aaggagcatt atgcttacgg ggaaatcaag
     1921 gtcatggctt ctgatgatga acacatgggc gtatttttag aattaaaagg ggcaggctca
     1981 cgcaacatgg aatatgtctt acaggcacag aacagggact ggtattcatt cttaaaccgc
     2041 tgtcttgact gtggcggtgt tatccgacgt tttgacttgg caatcaatga tatgtgcggt
     2101 ctgctggata ttcctgtgct atctgaaaaa tacagaaatg gtggtgcgga atgtcgctgt
     2161 aaaaactatg aaaatgtaca aggtggaaaa ttaagcggaa aaaaccgtaa tttagcaagt
     2221 accctttata tcggctcaaa ggcaagcaca aaatatttct gcctgtatga aaaacaaaag
     2281 gaacaggcaa cgaaaaagaa acataccgat attatcaacc gttttgaaat tcgtctgcgt
     2341 gacaaaaagg cggtacaggc agttgaagaa ctgttattga cgtataaccc tcatgggctg
     2401 gtgttttatc tcattaccga ttttgtggaa tttcctgact atccattgtg ggagatattc
     2461 atttcccatg acagtttacc ttttgaaatg aaccctgtac ctgtcaatat ggaacgcact
     2521 ctgcaatggc tggaaagaca ggtcatgccg tcaatcgtga tgatagagga aatcgacagg
     2581 ctgacaggtt caaactacat gaaaatgatt gatgaatgta cccacctttc tgaaaaacag
     2641 gaaatgcttg tggaacagat gtgtacggac atatcagacg taatcgaaag tgagggggtg
     2701 ttttatgagt aatacacagg atattcccgt atgggaaaaa tacaccctta ccattgaaga
     2761 agcgtcaaag tatttccgca ttggagaaaa caagttaaga cgattggcag aggaaaacaa
     2821 ggacgctggc tggctcatta tgaatggcaa ccgcatacag attaaacgcc gacagtttga
     2881 aaaggttatt gataaattgg acgcaatcta atgcaaatga gccttgtatg tgttatgatg
     2941 aacacaagtc atatcaaggc tctttccaac aaggaaagga gcagacacca tgaaagaaaa
     3001 aagacgggat aacaaaggtc gtatcctgca tactggagag agccaacgaa cagacggaaa
     3061 atacttatat aaatatgtag acgcattggg aaacacaaaa tatgtgtatg cttggagatt
     3121 gacacccaca gacccgacac caaagggaaa acgggaaaaa ccctcacttc gagaactgga
     3181 acagcagata agacgggata ttgaggacgg tatcgacagc acaggcaaga aaatgacgct
     3241 ttgccaactc tatgccaaac agaacgcaca gaggacaaat gtgaagaaaa gcacacagaa
     3301 acaacgggaa caactcatgc ggttattaaa agaggacaag ttaggtgcta ggagcattga
     3361 tacgataaaa ccctctgacg ctaaagaatg ggcgttacgc atgaaagaca aaggcttttc
     3421 ctacaatacc attaacaacc ataaacgctc gttaaaagcg tcattctaca tagccataca
     3481 agatgattat gtgaggaaaa acccttttga tttcaagcta agtgaagtca tagaaaatga
     3541 taccaaagag aaagtcgcat tgacagagga acaggagcaa gccttattgt catttattaa
     3601 gacggacaac gtgtatcaca agcattatga tgatgtgctg atactgttaa agacaggact
     3661 tcgtatctcg gaactgtgcg gactgacagt agccgacatt gatttcaaga atgaagttgt
     3721 gattatcgac caccagttac taaagagtaa ggaacagggc tattatattg aaacgcctaa
     3781 gacgaaaagc gggataaggc aagtgccatt aagcaaagaa acgatacagg catttcaacg
     3841 ggttatcaac agccgaccaa agataaagtc gatagaggta gacggacaca gcggttttgt
     3901 gtttatcaat ccgaaaggta atccaaaagt tgccattgat tataacgcct tatttatccg
     3961 tatggtaaag aaatacaaca agcaccacaa ggacaatccc ttgccacata ttacaccgca
     4021 tacgctacgc catacattct gcacaaggct ggcaagcaag aacatgaacc cgaaagattt
     4081 acagtatatc atggggcatt caaacatcag tatcacaatg aactggtacg ctcatgcgtc
     4141 catagatacc gcaaaatcag aggttcagcg tctaatcgca tagaagtatt taccacgatt
     4201 ttaaccacga ttgacaggga aaatataaga agatagacct agatatgcga ggtttaccac
     4261 aaaagcaaaa tgcccgtaga gccgataaaa taaggctttg cggacatttg agaagatata
     4321 aaaagatagt caaaaagaca tatataattt ataataatta tgactcttaa ttaaagtagg
     4381 tgcccatatg cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc
     4441 actgatatta acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc
     4501 aaatgaaaga agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat
     4561 ttcatttgtt atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt
     4621 accgatcttg attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga
     4681 gctgacacct aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt
     4741 agcagatata cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg
     4801 gcttagtttt cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat
     4861 tgacggtttg gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat
     4921 cattggatat tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct
     4981 tgtagcaagt attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg
     5041 cgatacagga gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa
     5101 gaacgtgact tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga
     5161 cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa
     5221 aaaacatctt catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac
     5281 gatttatgcg atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag
     5341 aatcggaacg atcatattga ttattgccat gttgtttgct gcaattttat tatttgaatt
     5401 gattggtctg atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa
     5461 taaagagtac cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga
     5521 tgaataacaa attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa
     5581 tttttcggac actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc
     5641 gaatttttga caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat
     5701 atccctacat cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc
     5761 acaatctgat agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg
     5821 atgaagagac agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt
     5881 tagcacctaa gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg
     5941 ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca
     6001 aacgactggc tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga
     6061 tgatctcagg tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg
     6121 atgagcgttt cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag
     6181 gttacaagtt attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc
     6241 atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt
     6301 gggggtggcg ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca
     6361 actattcagt caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat
     6421 tcagcttatt atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga
     6481 aaatatcgtt tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc
     6541 gttgtctcaa gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct
     6601 agtcgacgat tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc
     6661 agttccgatg attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat
     6721 caatcgtaca gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat
     6781 caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc
     6841 tttttcttta gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac
     6901 tgtggagatc cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt
     6961 ttcaagtaat cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga
     7021 tcaaaggcat ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt
     7081 gaccgtcaaa aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa
     7141 ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag
     7201 agcagttaga tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg
     7261 atgggtcagt agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa
     7321 tccagctttt ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc
     7381 agacgaaagg agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag
     7441 tgaaaaagat cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt
     7501 taatcattgt ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt
     7561 ccaaagaagg atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt
     7621 tccggtcttc tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata
     7681 tgtggattgg tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt
     7741 accgaagaca tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa
     7801 tttcttggcg tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat
     7861 aaaagatgtt taaatcctta atatttccat gacaaatata acaattgatg agataataac
     7921 aaagtgaaat ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg
     7981 ggggcagcgg aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga
     8041 tttatgacaa accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag
     8101 aaattctgat tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg
     8161 gaaatgattt aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac
     8221 aagcgtttat cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg
     8281 acaatattta ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata
     8341 caggcgcgac agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat
     8401 ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt
     8461 acgctgtaac tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca
     8521 aaccttcaga acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag
     8581 gaaaactatc cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg
     8641 aatcattgct tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag
     8701 tggcttgttt ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg
     8761 cccttgcaca gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg
     8821 aataaggagt ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta
     8881 gaaatggacg tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa
     8941 tttgcagaac atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa
     9001 gctggtgtcc ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt
     9061 cgggtagtca ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact
     9121 tatggtcaat gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct
     9181 aaaggatatg cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt
     9241 gataattact ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg
     9301 attgattggc caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg
     9361 acattaaaag aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg
     9421 agaaattttc ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat
     9481 ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg
     9541 acttatgcag gaaacaaaga gaacttggct ggacttccta gcgatcgcgt agaattggtc
     9601 gtaggagatg ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt
     9661 gttcattacg cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta
     9721 caaacgaata tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc
     9781 tatcatcacg tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca
     9841 ggacatgggg aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt
     9901 ccatattctt ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt
     9961 ggcttacgtg caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa
    10021 aaatttattc ctcgccaagt aacgaatatc ttaagcggta ttcgtccaaa attatatggc
    10081 gaaggaaaga atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg
    10141 attttgacga aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat
    10201 aataagaccg tgatggaatt gatcttagaa atgatgggtc aaccaaaaga tgcgtacgac
    10261 catgtaaacg accgtgcagg acacgacttg cgttatgcaa tcgattcgac aaaattacgg
    10321 gaagagttag gatggaagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc
    10381 aaatggtacg aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca
    10441 tatgccaaaa atggacaata atacagagaa aatgtcactg tattaatgga aaaacacatg
    10501 tgaggcaggc gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataatcatat
    10561 gtttttatta actggaggaa atgggcaact aggtacagaa ttgcgccatt tattagatga
    10621 aaaaaatctg caatatgttt caacagatgc aaaagacttg gatatcacag atgccgagaa
    10681 aacaatggct tacatcacag aattaaaacc tgatgttatt ttccattgtg cagcttacac
    10741 agcagtagat aaagcagaag aagaagcaaa agaattagat gagaagatca atgttgatgg
    10801 tacaagaaat gtagcacttg cagcaaaagc tgtgaatgct caattcgtct atatcagtac
    10861 agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa
    10921 tccactaaat gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt
    10981 agaagactat tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt
    11041 cttcacgatg caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt
    11101 tgggcgacca acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa
    11161 agcaccattt ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgaattcgc
    11221 aaaagaaata ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc
    11281 gcaaaaagca acacgtccgc agtattcagt catgagcctg aaaaagacag aagagttagg
    11341 atttgtcata ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat
    11401 gaataagtaa tttcctttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag
    11461 aagtgcacga cgaaaagaaa agccaaaagc gaaccaggca gattcgaatt tggtgcttga
    11521 cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt
    11581 taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg
    11641 gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt
    11701 gcagacacct ttttacctct cacaagctga agaggacttt ccgttggtga acgaggatta
    11761 ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt
    11821 tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt
    11881 ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt
    11941 aacgaagaga aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc
    12001 agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat
    12061 gactgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat
    12121 gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt
    12181 gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa
    12241 agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg
    12301 agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt
    12361 atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt
    12421 ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc
    12481 cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa
    12541 aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat
    12601 cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt
    12661 tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt
    12721 tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc
    12781 agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc
    12841 ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt
    12901 tggctggaga cgcttgtttt acctcgcttt tgccgcctta gttatcgtat ctggcttttt
    12961 cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat
    13021 ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa
    13081 cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc
    13141 tgcatggaag aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca
    13201 tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt
    13261 ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa
    13321 acgaccatta actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc
    13381 ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca
    13441 aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg
    13501 aaatgttagt agaaaggaga gtggagataa ttcaagcaat cgtacaaaaa tttgtcaaca
    13561 aaatgagtgt cggtttcttg cttttgttac tgacgctagt cttttttgtt tggacagcag
    13621 ggttaggttt tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta
    13681 gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc
    13741 taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt
    13801 ctttatttgg agacttactg tttcctttga ttattttatt ttgtttagta attaaatgga
    13861 tcgagtggac aaaatgggat cgcggtattt atctgattgt cttttcggtc ttcctcggaa
    13921 tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc
    13981 caggcaattc gctttctgat atttggatca atacgaatat aatcggaagt tctttgatga
    14041 ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat gtacgaatca
    14101 tcggtgcagt agcagttgtt gcagcttttc tcgggatttg ggtctgccaa tctagaggtg
    14161 cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg
    14221 tcattcgtgc gccatttatt gcttacacgg ttacgattct tttagctttg ccgatttctt
    14281 atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat atttggcata
    14341 aattctatca gacgctagga gaaaaatctg aacaaatttt actaggaatg aaaacattta
    14401 tcttccaacg aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata
    14461 tctatggatt gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct
    14521 taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg
    14581 tgatgatgca gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt
    14641 atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct
    14701 atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac
    14761 cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag
    14821 aggctgtgac aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta
    14881 aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca
    14941 gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg
    15001 aggaacacaa atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa
    15061 attattgcta cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg
    15121 aatcttatgg gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg
    15181 tgttggactg aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac
    15241 cgggatgatt ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg
    15301 ggaatatact tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa
    15361 aatcctatcc aatctgtaca cccacttatt cttcattctg attggcatag ctgtcgcgct
    15421 tataaatggt atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat
    15481 gtgtgctttc ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga
    15541 tatcatgcaa ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg
    15601 gagccctaat cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta
    15661 tgttgttcaa ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac
    15721 atatactttg tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt
    15781 ccgtagattg aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat
    15841 gcaattagac taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga
    15901 tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa
    15961 aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg
    16021 ggaaaatcta caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg
    16081 gaaatcgaag gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat
    16141 tctggctatg aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc
    16201 gatgaaatgg tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct
    16261 gtaaaaactt attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg
    16321 aatcccgata ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta
    16381 aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct
    16441 catgatatca gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa
    16501 gtcgtcgaat atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa
    16561 aaatcagtta aaacagtgga tcggaataaa aaccaagtag aacggttcgc cgctccagat
    16621 atcgtcgacg tgacgaaagc ggaattgttg gataaagaca aaaatccggt ggatatgctg
    16681 gaacaaggag aaaaactata cgttaaagta acttatgaag taaaagatga tacaatcaaa
    16741 aatccggttt taggaattgc gctacgaaca gtcgacaatt tttatgtatg cggacttaat
    16801 actctactag atggtatcaa gattccttgg aaaaaaggag agaatatctt ttatctggag
    16861 tatgaaaagt taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa
    16921 aatgcaacgg ttcctttggt atataagaca aagtacatga acttgtttgt cagcggttct
    16981 tatatcggag aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat
    17041 gaaatatgat tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca
    17101 gatcaaacca ggaagtaaag ttttagagtt tggtcccgga aacggccgat taacgaaaca
    17161 tttaatcgga gcaaaacaat gtgaagtgag cattgttgag ttagataagg aattgttcga
    17221 ttttgtcagc gaatttgctc aagatggatt ttatggtgat atcgaaagtt ttgagtgggc
    17281 aaactattat gctggacaga catttgatta cgtcttgttt gcggatgttt tagaacattt
    17341 ggtagatcct ggaaaaacat tgaagaaagt cagagaattt ttgaatgaag aaggagagat
    17401 cctgattaca ttccctaact tggcgcataa ttctgtcatg atcgatttgt tcaataacca
    17461 attgccgtgg gcttcttatg gtctcctaga tgaaacccat aattcttttt atactcatga
    17521 tggcttccaa aaagtttttg aaaaagcggg attattcatt aatattgaag attatttgta
    17581 cttagctgta ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg
    17641 ctatgatttc aaaatgcgac catttggtga agtctaccaa tactttttct cattgatgaa
    17701 acatccagta gcacaaagtt ccattgcaga gccacaaaat tctaattatg taaaagtgtt
    17761 agaagtaacc cagcaaacga aacaagatga aacaatccaa caaataccat tcaataattt
    17821 cacaggggaa aatgagacac taagtttccc cgtatcagaa acaacagaaa gaatggtgtt
    17881 tcgcttcgcg caacaaccta gctttatcga attcagcgca gaagctgccg gagaaaaact
    17941 aacatttatc gattcgaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg
    18001 gaaagaatta ccagagttcg ttctaacgga tatttctggg aaagaagtga ctatccattg
    18061 tcactatcgc tttatcgggg aattgacacc aacaatgaaa gaattgttgg aaacgattcg
    18121 tcccatggct gaagtcacaa agcagctttc tgagaaaaat gacgaattag aaagagaaaa
    18181 tcatcatctt cttgaggaaa atacaagact agaccatacg ttaaaaacaa ccacaaatcg
    18241 ctattgcaca ttgttggaaa gtgatgaatg gacaataaaa catagactag gacgacgtaa
    18301 gaaagaaact tcgaaaaaaa tccaagaaaa agaactttct atatgtattg acgagaaaat
    18361 ctgggatgca gaaacaaaaa tattaaaaat catcggatgg ggaatcgcta attcggatcg
    18421 ccagcccttg tcctataagt tatcagctga ccaatcgcca tttttcgaag caattcctgt
    18481 ttcacgagaa gaagtcaatc aagcagaaca gctcgcaaaa ggaacagaag cgggatttga
    18541 gttacgaatc ctttgcgagc aagaacgttc attcttagtg gaagcagtag ctcaaaatgg
    18601 ccaatcatgg atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga
    18661 atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact
    18721 ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata
    18781 aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt ggatatcaat
    18841 catttatata atcttgatgt taagacccaa agcggtttta aggtacgttt atcagggaaa
    18901 atgagaggaa aagccatctt agacttccaa acagcgagac atcaaaatgg catagctgtg
    18961 aaattaaacg gtaaataccc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa
    19021 agattactga aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcgatccgt
    19081 cgggtcaaat tggaattgaa gccaggatcg atcgactatg cagagtggat cagtcgtcat
    19141 gagaaaccag atttgaaaga gcaaaggaaa gaagcacaag gatttgcgta ccgtccattg
    19201 atttccatac ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat
    19261 tcggtactgg atcaaacgta tgatcattgg gaactatgta tcagtgatga tgcttctact
    19321 gatccagcta tccgaaagtg tctggaatcc tatcaagcaa aagatgatag aatcaaagtc
    19381 gttttccgaa aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatagca
    19441 gaaggagaat ttatagcact cttagataat gatgatgaat taccgccatt tgcattatat
    19501 gaagtggcaa aagtgctaaa cgtacatcca gaactggatg tgatctacag tgacgaagat
    19561 aaaatcgatg cagatggcaa tcgctttgat ccgcatttca aagcagactg gtcgcctgat
    19621 acattgatgg gaaataatta tatctctcac ttaggcgtat atcgaacaag cattgttaaa
    19681 gaacttggcg gtttccgtaa aggctatgaa ggttctcaag attatgattt ggtccttcgt
    19741 gtgacagaac aaatccctgc agatcatatt taccacattg accgagtctt gtatcattgg
    19801 cggacgatcc caggatcaac tgcaagtaat ggcgaagcca aaagttatat ctatgattcc
    19861 ggagtgaaag cattgacaga tgccctgtct agaagaaaca tcaaaggaag cgtacgtcct
    19921 ggcagaattt ctggatttta tgagattgct tatgacgtct tgcaagagga tttggtcagt
    19981 gtgatcattc caacgaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc
    20041 gaaaaaacgt cttattcaaa ttatgaaatc attattgcgg ataacggaag tacagatcca
    20101 aaaatgcaag aattatttgc agaatacaag catcagctga aagaccgctt tatcgtagaa
    20161 ttgattgaca ttccattcaa ttattctcga attaacaatt tagcagcaga aaaagcaaac
    20221 ggcaaatact ttttattctt gaataacgat acagaagtga ttgagccaga ttggatgacg
    20281 gtgatggttt cttatgcaca gtttgatcga atcggctgtg taggagcaaa actgttttat
    20341 ccagatgata caacgcagca tgcaggagta ttattaggaa tcggtggtgt tgcagggcat
    20401 gcattgaata actatgatcg aacacattgt ggttatttcg gccgattggt cattgacgtc
    20461 aattacttag cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtt
    20521 aacgggtttg atgaaacatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt
    20581 tatgaactgg gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct
    20641 aaatcaagag gttatgaaga tactccagaa aaacaaagac gttttgctgg tgaaatcaaa
    20701 aaaatgcaag ataaatggcc agcatatatt gcgcatgatc cattttacaa tgataattta
    20761 acaaaagaag gaatcggcga cttttcgttg agaccagatt aaactataat aaaaagctgt
    20821 tatgttgtga ttcgctcgca cataacggct ttttatagtt ttttgtcaaa aaaaggcaga
    20881 aggtgccgac tcaaaaaaat aagtcgaggt tgggacagaa gtgtttaact ccgagaaata
    20941 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcggctta tttaagaaga
    21001 agttgcttct gctcccaccg tttattcgtt tttaaagaat gagacaaaag tgtttcttac
    21061 ttttgtccca cgctcttatt catgctcaga gcgccaatag atacaaaatc atagccaaag
    21121 tttcacgaac cctagctgtc aaagaatcga tattttattt taatataggc tattttaaaa
    21181 taaatggttt aaggtattcc tctacaaaag ccactctatc aattatcatg ggtttaaaat
    21241 aagaagtgat ggagataacc agattgttct tagcgtcaat ataaagaaca tttccactat
    21301 taccaattgc tgaatatatt tcgttctcag aatctaatac ccaccataaa tagccatatc
    21361 tcatattttg taagtcttct aaagcactgg ttctaggttt gaacatctcg tttatccaca
    21421 tgggtggtac gatttgtttt cctttataca tccctttatt taggcataat acaccaattt
    21481 ttgccagatc acttgccgat aaacacaaac catatcctgc cgtcccgata tttagtggat
    21541 cacaaaacca gacattggtt ttaggagact tatccattgt aaactgctta tgttcttcag
    21601 ctgtttcagc ataataattg acatggtttg gtatccctat aggatcaaat agatacttat
    21661 tggcaaaatc aacagttgtt agcttacttg tctgatacaa caagccagtt aatatgtgta
    21721 aacagacagt ttgatagttg aattcgccgg ttataccttt tcttcctccc aataaatcta
    21781 gtgatgtttt tgtccaatct tcacttgaac agacttttgt ccatggatcg cctttacatt
    21841 tgtagggtgc tttcattgtt agcaagtgct tgattgtaat agcgtaaata gttttctccc
    21901 cgcgtttcac tttatactct ggaaaataat ctaatacttt atcatcgata cttttgatta
    21961 atccttgctc taaagcaatg ccaatcagca aagatacaat acttttcgtt gctgacatga
    22021 tatgacaagt atcgtctttt ttataaccat tccattcatc tgtataaact tctaccccat
    22081 ccttatatgc gacaatttga catatatttg gttgtcgaaa accaataaaa tcatgcaact
    22141 cttgtttatt cataagatat tacctcactt tcttctataa gcataatgtc attaaaaatc
    22201 gatttcaatt ttttcgattc ttcacgtaat acaggtattc ttatggacta gacagcgctt
    22261 tgggaagatg gattctctga aaaatataaa ggttcacttt atgagtggat aaaaaaagca
    22321 gcaaatactt cgacttcttt aatttaccaa cattagtgaa aatcgattca tctggagatt
    22381 tgtaatttag agtccttgtc ttgattagag gtgtataaag ctttttatta gacattttta
    22441 aattccatta caatttgctt ataaactagg tttttaaagg tgactatggt aagataatag
    22501 agatattatt ttgtgattag gagtgtatat atgaataaaa atggagaatg gagtgctgca
    22561 cgacggatat tcattata
//
LOCUS       DAZKAO010000038        21843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000038 DAZKAO010000000
VERSION     DAZKAO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.038"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..607)
                     /locus_tag="ACG8H8_001189"
     CDS             complement(62..607)
                     /locus_tag="ACG8H8_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP8986275.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(619..1311)
                     /locus_tag="ACG8H8_001190"
     CDS             complement(619..1311)
                     /locus_tag="ACG8H8_001190"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP8986276.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1330..1614)
                     /gene="macP"
                     /locus_tag="ACG8H8_001191"
     CDS             complement(1330..1614)
                     /gene="macP"
                     /locus_tag="ACG8H8_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP8986277.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1627..2190)
                     /locus_tag="ACG8H8_001192"
     CDS             complement(1627..2190)
                     /locus_tag="ACG8H8_001192"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP8986278.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2418..3080
                     /locus_tag="ACG8H8_001193"
     CDS             2418..3080
                     /locus_tag="ACG8H8_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP8986279.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3204..4400
                     /locus_tag="ACG8H8_001194"
     CDS             3204..4400
                     /locus_tag="ACG8H8_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP8986280.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4501..5877)
                     /locus_tag="ACG8H8_001195"
     CDS             complement(4501..5877)
                     /locus_tag="ACG8H8_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8986281.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5914..6780)
                     /locus_tag="ACG8H8_001196"
     CDS             complement(5914..6780)
                     /locus_tag="ACG8H8_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP8986282.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6869..7894)
                     /locus_tag="ACG8H8_001197"
     CDS             complement(6869..7894)
                     /locus_tag="ACG8H8_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8986283.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7912..8586)
                     /locus_tag="ACG8H8_001198"
     CDS             complement(7912..8586)
                     /locus_tag="ACG8H8_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8986284.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8770..9663)
                     /locus_tag="ACG8H8_001199"
     CDS             complement(8770..9663)
                     /locus_tag="ACG8H8_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8986285.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9737..11434)
                     /locus_tag="ACG8H8_001200"
     CDS             complement(9737..11434)
                     /locus_tag="ACG8H8_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8986286.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11427..12740)
                     /locus_tag="ACG8H8_001201"
     CDS             complement(11427..12740)
                     /locus_tag="ACG8H8_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP8986287.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12740..16681)
                     /locus_tag="ACG8H8_001202"
     CDS             complement(12740..16681)
                     /locus_tag="ACG8H8_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8986288.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16790..17248)
                     /locus_tag="ACG8H8_001203"
     CDS             complement(16790..17248)
                     /locus_tag="ACG8H8_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8986289.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17546..18064)
                     /locus_tag="ACG8H8_001204"
     CDS             complement(17546..18064)
                     /locus_tag="ACG8H8_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP8986290.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18066..18683)
                     /locus_tag="ACG8H8_001205"
     CDS             complement(18066..18683)
                     /locus_tag="ACG8H8_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP8986291.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18680..19534)
                     /locus_tag="ACG8H8_001206"
     CDS             complement(18680..19534)
                     /locus_tag="ACG8H8_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986292.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19531..20442)
                     /locus_tag="ACG8H8_001207"
     CDS             complement(19531..20442)
                     /locus_tag="ACG8H8_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986293.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20483..21817)
                     /locus_tag="ACG8H8_001208"
     CDS             complement(20483..21817)
                     /locus_tag="ACG8H8_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8986294.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6339 a         4803 c         3769 g         6932 t
ORIGIN      
        1 ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt
       61 tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa
      121 cgggatcgaa acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg
      181 catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg
      241 tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc
      301 gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag
      361 agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct
      421 ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt
      481 gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc
      541 cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg
      601 cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga
      661 tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt
      721 gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac
      781 agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat
      841 tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa
      901 attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata
      961 tagaggcatt cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa
     1021 ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc
     1081 agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt
     1141 aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa
     1201 tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc
     1261 tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca tctttttaat
     1321 tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat
     1381 aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct
     1441 tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt
     1501 ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg
     1561 tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg
     1621 atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc
     1681 atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa
     1741 ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc
     1801 atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg
     1861 gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc
     1921 gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc
     1981 taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat
     2041 cccgacaccg ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg
     2101 aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc gactgattgt
     2161 tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac
     2221 tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac catagataaa
     2281 ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc
     2341 cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat
     2401 gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc
     2461 tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg
     2521 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
     2581 ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
     2641 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
     2701 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
     2761 cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
     2821 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
     2881 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
     2941 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
     3001 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
     3061 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
     3121 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
     3181 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
     3241 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
     3301 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
     3361 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
     3421 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
     3481 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
     3541 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
     3601 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
     3661 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
     3721 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
     3781 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
     3841 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
     3901 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
     3961 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
     4021 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
     4081 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
     4141 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
     4201 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca
     4261 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
     4321 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
     4381 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
     4441 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
     4501 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
     4561 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
     4621 acaagtcaaa atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
     4681 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
     4741 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
     4801 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
     4861 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
     4921 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
     4981 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
     5041 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
     5101 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
     5161 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
     5221 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
     5281 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
     5341 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
     5401 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
     5461 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
     5521 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
     5581 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
     5641 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
     5701 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
     5761 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
     5821 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
     5881 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
     5941 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
     6001 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
     6061 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
     6121 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
     6181 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
     6241 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
     6301 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
     6361 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
     6421 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
     6481 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
     6541 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
     6601 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
     6661 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
     6721 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
     6781 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
     6841 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
     6901 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
     6961 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
     7021 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
     7081 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
     7141 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
     7201 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
     7261 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
     7321 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
     7381 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
     7441 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
     7501 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
     7561 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
     7621 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
     7681 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
     7741 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
     7801 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
     7861 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
     7921 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
     7981 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
     8041 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
     8101 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
     8161 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
     8221 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
     8281 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
     8341 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
     8401 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
     8461 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
     8521 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
     8581 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
     8641 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
     8701 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
     8761 attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
     8821 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
     8881 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
     8941 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
     9001 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
     9061 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
     9121 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
     9181 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
     9241 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
     9301 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
     9361 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
     9421 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
     9481 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
     9541 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
     9601 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
     9661 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
     9721 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
     9781 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
     9841 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
     9901 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
     9961 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
    10021 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
    10081 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
    10141 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
    10201 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
    10261 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
    10321 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
    10381 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
    10441 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
    10501 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
    10561 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
    10621 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
    10681 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
    10741 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
    10801 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
    10861 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
    10921 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
    10981 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
    11041 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
    11101 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
    11161 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
    11221 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
    11281 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
    11341 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
    11401 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt
    11461 aataaaacag cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca
    11521 ttcgtttgcg ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc
    11581 tctttcggtt tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat
    11641 tcataggcag agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact
    11701 actacatatt gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt
    11761 ctcttacttg cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag
    11821 gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat
    11881 ttgcttacat ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc
    11941 aagtcattag caggagccat atctaaataa tatttccccc cttctatcat ctgatataga
    12001 gcaaaaacag caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac
    12061 ttgaagaagt atggatctct tagataaccg acatttttag gatagatatg aatctcttgc
    12121 agttcctcgg ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct
    12181 gtctgatcga tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa
    12241 taaactgcat cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct
    12301 aatccgccaa ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct
    12361 tctgtgatta gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct
    12421 ttaggagtca tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg
    12481 gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct
    12541 tcctcagaga tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata
    12601 tctcgaatca tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc
    12661 gacatcgcat gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc
    12721 aaacacaaca gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac
    12781 cataggctgc tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc
    12841 cggatcctcc tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc
    12901 tgattcgatt tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg
    12961 cttcttcgtt tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa
    13021 ctacccgatc attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac
    13081 ctggaagttt tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca
    13141 acaagtctga agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga
    13201 tgatttcata ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac
    13261 gtcggctatt catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt
    13321 ctaaggttac ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt
    13381 cgtcttcgat gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga
    13441 acacttgatc agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac
    13501 caatagacag atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg
    13561 cacgagaata gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat
    13621 taccagtgta tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac
    13681 ctgcaccatc taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat
    13741 caaacgtatt gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg
    13801 tgagatattg ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat
    13861 cgtaaagtcg caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct
    13921 ggctctcaat gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa
    13981 atgaatctgg ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc
    14041 cgtcttcatc tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac
    14101 cagaaagccc tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa
    14161 gttcaaactc tgcgccattg attggcatat tactgccttc aattagttta ttgatattca
    14221 tgtggattgg cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg
    14281 tgccttctat gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg
    14341 aattcggatc tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa
    14401 gctcgtagtg acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat
    14461 acgtctcatc tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt
    14521 cgctgactgc tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc
    14581 cgtccgtggt agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt
    14641 catataatgc aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt
    14701 ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag
    14761 tagtgatatc attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc
    14821 aaaaatttcc gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt
    14881 ccccttcacc taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg
    14941 ttgtcggagt cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt
    15001 ctggctcttg gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg
    15061 ccgatctgcc agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt
    15121 gctggatcgt tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct
    15181 tgttgttcgc attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt
    15241 tcaatacatt tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc
    15301 ctaaaccaat ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat
    15361 gataagcaac tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat
    15421 tgttattaga agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct
    15481 ctccataacg atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg
    15541 taactggatg agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga
    15601 acaaaacgtc attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc
    15661 cattagtgct cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat
    15721 ctcccacaac gattaggatt ttttctggat ttctattttc tccgccattg tctttataca
    15781 atgtttcata gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat
    15841 ttaatgctgt attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt
    15901 ccgaactgat ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac
    15961 cgattcgaat atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct
    16021 caatcaacgc agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg
    16081 ctgacttatc tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga
    16141 tgacatctag atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg
    16201 tgctgcttgg cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt
    16261 aactgttttc aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc
    16321 tatcttcttc gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg
    16381 ctcccccgtt tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt
    16441 tgtctccttg atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat
    16501 tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat
    16561 attcataggg gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact
    16621 ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca
    16681 tctcattttc ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt
    16741 gtaattcttg gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt
    16801 tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat
    16861 aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat
    16921 cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg
    16981 ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag
    17041 ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt
    17101 cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg
    17161 tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact
    17221 gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt
    17281 ctttcacaac aatcaacaaa acaatgccca tctaattata atttaattta attttaattt
    17341 taaatcaaat gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa
    17401 attaaattat aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat
    17461 gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca
    17521 ctcatgcttt attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac
    17581 ttcctaaaat agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg
    17641 aataagcgat caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga
    17701 acaacgcagc tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct
    17761 tcggtttact tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg
    17821 tcgttttttt gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg
    17881 ctagaccata ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa
    17941 taaaaccacc attagagata ttactgataa atttcaaaaa attgattttt cgactatccg
    18001 ttttccgttg cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca
    18061 acatgttatt cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact
    18121 tgaaaataaa acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag
    18181 aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta
    18241 aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt
    18301 ttgtaactaa agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta
    18361 tagccaattc ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa
    18421 taaacacttg ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc
    18481 cagaatagtg aaccgagctt tttctcttca ttttttttgt aatgataaat catatacaga
    18541 actgttcgac ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc
    18601 tgtaaatgga taagacgtgg aatccattca atgatttcta agaaataatc attggctttt
    18661 ttaccaatcg atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga
    18721 aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg
    18781 ccatcagata attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta
    18841 acgtgtattt ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg
    18901 cgttgaagga attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat
    18961 gactccaaat atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat
    19021 tgatcgataa gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg
    19081 gaacgatcag gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag
    19141 ggatcatcgt tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct
    19201 ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat
    19261 taccgatcac tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa
    19321 aggcggcaat cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag
    19381 gataacgaac gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa
    19441 tgatcaaaat gattcctatt actgtcatga ggacataact tagtattttt tttgtttgcc
    19501 gattcacact ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac
    19561 ccacttaggt gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat
    19621 cattgacata gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg
    19681 cagattataa aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata
    19741 agcttcttga aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat
    19801 aggagtgatt aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat
    19861 ttctgctgat tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat
    19921 tgctcctcca gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc
    19981 aaacagccaa gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc
    20041 agatggagaa agcagctgca tccataagat gtagactgct actcctgata agactgccgg
    20101 taaatagaaa gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc
    20161 taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt
    20221 gtataacgat ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc
    20281 aatgaaattc atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag
    20341 cataggccct agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc
    20401 ccatcttgct tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg
    20461 tacctagaca gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt
    20521 gtgcatctgc caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt
    20581 cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt
    20641 taaagttttc gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat
    20701 tcactaaatt ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt
    20761 tttcttgtac ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt
    20821 ttgctccgta tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt
    20881 cttctttttc tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca
    20941 aactagagta ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat
    21001 cagatacacc agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat
    21061 cttgccagtc ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt
    21121 tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc
    21181 ctaaattcca taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta
    21241 gtttacgtgc tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc
    21301 cagcttcttt aaataacttt ttattataaa aaatgacttg tgtatctgta ttgaatggga
    21361 tcgcataagg atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag
    21421 aagtgtcttt ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg
    21481 caacggaatt tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata
    21541 aagatttagt ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt
    21601 gtgattgatt gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc
    21661 aaaaggacca gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg
    21721 cacttgaact ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg
    21781 aacttgccag caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt
    21841 aac
//
LOCUS       DAZKAO010000039        21220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000039 DAZKAO010000000
VERSION     DAZKAO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.039"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..949
                     /locus_tag="ACG8H8_001209"
     CDS             53..949
                     /locus_tag="ACG8H8_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP8986295.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1099..2040)
                     /locus_tag="ACG8H8_001210"
     CDS             complement(1099..2040)
                     /locus_tag="ACG8H8_001210"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8986296.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2166..3287
                     /locus_tag="ACG8H8_001211"
     CDS             2166..3287
                     /locus_tag="ACG8H8_001211"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP8986297.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3545..5047
                     /gene="eat(A)"
                     /locus_tag="ACG8H8_001212"
     CDS             3545..5047
                     /gene="eat(A)"
                     /locus_tag="ACG8H8_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP8986298.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5114..5641
                     /locus_tag="ACG8H8_001213"
     CDS             5114..5641
                     /locus_tag="ACG8H8_001213"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8986299.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5852..6913
                     /locus_tag="ACG8H8_001214"
     CDS             5852..6913
                     /locus_tag="ACG8H8_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP8986300.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6916..7812
                     /gene="murQ"
                     /locus_tag="ACG8H8_001215"
     CDS             6916..7812
                     /gene="murQ"
                     /locus_tag="ACG8H8_001215"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP8986301.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7826..9808
                     /locus_tag="ACG8H8_001216"
     CDS             7826..9808
                     /locus_tag="ACG8H8_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP8986302.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9873..10319)
                     /gene="nrdI"
                     /locus_tag="ACG8H8_001217"
     CDS             complement(9873..10319)
                     /gene="nrdI"
                     /locus_tag="ACG8H8_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP8986303.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10530..11954
                     /locus_tag="ACG8H8_001218"
     CDS             10530..11954
                     /locus_tag="ACG8H8_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP8986304.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12135..12908)
                     /locus_tag="ACG8H8_001219"
     CDS             complement(12135..12908)
                     /locus_tag="ACG8H8_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8986305.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12905..13810)
                     /locus_tag="ACG8H8_001220"
     CDS             complement(12905..13810)
                     /locus_tag="ACG8H8_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986306.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13964..14284)
                     /locus_tag="ACG8H8_001221"
     CDS             complement(13964..14284)
                     /locus_tag="ACG8H8_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP8986307.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14758..15444
                     /locus_tag="ACG8H8_001222"
     CDS             14758..15444
                     /locus_tag="ACG8H8_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP8986308.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15446..16390
                     /locus_tag="ACG8H8_001223"
     CDS             15446..16390
                     /locus_tag="ACG8H8_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP8986309.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16589..17233
                     /gene="pcp"
                     /locus_tag="ACG8H8_001224"
     CDS             16589..17233
                     /gene="pcp"
                     /locus_tag="ACG8H8_001224"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP8986310.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17312..18589
                     /locus_tag="ACG8H8_001225"
     CDS             17312..18589
                     /locus_tag="ACG8H8_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986311.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            18847..20064
                     /locus_tag="ACG8H8_001226"
     CDS             18847..20064
                     /locus_tag="ACG8H8_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8986312.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(20385..21134)
                     /gene="pnuC"
                     /locus_tag="ACG8H8_001227"
     CDS             complement(20385..21134)
                     /gene="pnuC"
                     /locus_tag="ACG8H8_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP8986313.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         6814 a         3825 c         4463 g         6118 t
ORIGIN      
        1 atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa
       61 aaatgacgta ctgctagttg caaaaaataa atcactttat tttttgcctt ccatccgctc
      121 tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat cagctagaca
      181 acagttggca aattatcagc tgatcgtttt tttagattat ggatttgata tggagatggc
      241 tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga atcacttcaa
      301 agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg atgagattta
      361 tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt cattttattc
      421 gaaacatatg aatttttctt ctagcgaacc aaagacggat ctattttttg gtgcaacgaa
      481 taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa gagatgttac
      541 gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc ttacttatga
      601 agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc gggagggtca
      661 gcaaggagtg actttgcgga catttgaatc catgtatttt caaaagaaac tagtgacgga
      721 taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt tgcttcgaga
      781 acgtgattta gatgaattac cggaatttct gcatacacca tttaagccaa tcctatcaga
      841 gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta acgattccac
      901 tatttatgag caatacgaat atgacctctc ctctgttgaa aacaaataaa aaacctacat
      961 tttgaagaga ttatgtactg aaacaaacaa gcacaattct tatggcatca tgagagttgt
     1021 gttttctttt tgatctttta ttgggcttaa aaaaagagaa ccagctgctg attcagctga
     1081 ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat ggactaattg
     1141 ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc gccaaatggc
     1201 accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt ggtacataga
     1261 tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc
     1321 ttcttgatct agtatgattc cttcttttgc tgcgatagaa gcaaattcag atacgattgt
     1381 tcgaaccatg ctctctgccg ctttttgcgc gcctagttca gcaatattac aatccaaaat
     1441 cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg aataacgaac
     1501 gttatggcta tagatcggat ttaaatttgc ttcttgaaag acttctacaa ctttttttgt
     1561 aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac ctaacaatgt
     1621 gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata agatattttt
     1681 ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta aacacaaaac
     1741 ataagtattg tcagtaataa tggattggat cgaacaaaac atatcatcta attgattggc
     1801 ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt cttctggtga
     1861 atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca acccatttgt
     1921 acgaatggct tcaatgtgag caggccaacg atcgatcaag aggacctcat ttccgctttg
     1981 atgtagcatc aatccgattc ggctgcccat tgctccggcc ccagctattg cgattttcat
     2041 gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaat ggaagaaaat
     2101 agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga
     2161 tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct tcatttgaac
     2221 taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga
     2281 ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg ggaacatggc
     2341 gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat ttattgacaa
     2401 cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg ttagatctac
     2461 ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta ggagaactta
     2521 ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga ggaagcgggt
     2581 gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa gggtacacag
     2641 aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac gttcttgttt
     2701 ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt caacaaggag
     2761 ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct ttcttagata
     2821 aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat ttagatgata
     2881 ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg ggagcgaaga
     2941 tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag
     3001 cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac ggaaaagttc
     3061 catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa
     3121 gtagagacca agagctagaa ggactttctg attttctccc aattgtctta agtattcatt
     3181 tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa aaaacaaaaa
     3241 tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg acaaaatcat
     3301 tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt gcattgaaaa
     3361 attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa ggaagcagtg
     3421 tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa
     3481 cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg
     3541 gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc aaggcacatt
     3601 attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac ttatcggacg
     3661 aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac cttatcaagg
     3721 gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag ataaagaacg
     3781 tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa tcgaaagaga
     3841 gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca cactatcggg
     3901 gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc atttcccgtt
     3961 aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag cggcttattt
     4021 gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta tcgatgaagt
     4081 agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc
     4141 tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt
     4201 gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat ggtctcgttc
     4261 ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat ctagacgagt
     4321 gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca aagcaatcgt
     4381 gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata tcgaatatat
     4441 cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa gcgtagaaga
     4501 tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa tcgagcctca
     4561 tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta tccattatct
     4621 tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac atctgagcat
     4681 tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa
     4741 aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga tggaaagaga
     4801 tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag tggaattggc
     4861 taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga attatttgga
     4921 tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg ccatgttact
     4981 agtggaacat gatcaaacct tcctggataa agtatctact gagattattt ctcttgagag
     5041 aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact ttgccaacga
     5101 aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga acgcacaagg
     5161 attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc aggcacagaa
     5221 actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc attcccctct
     5281 tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt
     5341 tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa gcaaagatga
     5401 gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt
     5461 agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt
     5521 ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt ttcatccgat
     5581 gcctaatgag gggttcttta cattcatggt agataataca gaattggttt cttatgagta
     5641 attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc ttagcagtgc
     5701 cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga aatgaaattt
     5761 aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct agtcatagaa
     5821 atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat ttatgaacga
     5881 ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg gctttgtcgg
     5941 tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa agcgattgat
     6001 cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata tttcaggaga
     6061 ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag caattggtgt
     6121 aacaggttta agaatggatt atcatatttc caatcagcag attgccgact ggtcacatca
     6181 attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg aattgaaaga
     6241 agcagaagct gatttctcac agattgaagc ttggcataat tattatccac gtcccgaaac
     6301 agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct
     6361 tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc aaggattgcc
     6421 aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc tattagcaag
     6481 taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc aggagcaatt
     6541 tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag atgaagaatt
     6601 ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag atgtcatacg
     6661 aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata cagctacgcg
     6721 tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg gtgagatcca
     6781 attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta aagttatcaa
     6841 agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat ttataagaaa
     6901 agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga acgcagaaac
     6961 caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact aatgaacaaa
     7021 gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga accagtgatt
     7081 gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg agccggaaca
     7141 agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg tgtagaaccg
     7201 gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg
     7261 gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt gacaaaaaat
     7321 gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg tggattagat
     7381 tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca agccgaaatc
     7441 agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt gacaggctct
     7501 actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc tactatcagt
     7561 atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc aacaaatgaa
     7621 aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga ttacgaaaca
     7681 gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt catgattttg
     7741 acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg atttgtaaaa
     7801 gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg aagaactggc
     7861 aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg tccactgcat
     7921 gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg aattaaaagc
     7981 gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca tcggacctgg
     8041 gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat taggagaaaa
     8101 actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag accgtgtaaa
     8161 cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa
     8221 atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat ttgtcggtac
     8281 tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg gaacacttga
     8341 tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag
     8401 ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa caccaactct
     8461 tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag caccactgaa
     8521 aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag gtgtcatctt
     8581 cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt
     8641 agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg aaattttctt
     8701 catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca actgggtact
     8761 tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc cgatggttat
     8821 gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg aaactggtcg
     8881 tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg cagcattggc
     8941 attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag gagctttacc
     9001 agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac ctctaggacg
     9061 cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg
     9121 aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat tgatctctga
     9181 cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg gcttcgttgc
     9241 tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag cgactgaaac
     9301 agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg aaagtgtgac
     9361 tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa gtgatccagt
     9421 ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg gacatatcta
     9481 ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta ttgggatcac
     9541 aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg acttaggcgg
     9601 tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata cgcttgtagc
     9661 agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga tcgtcttggt
     9721 aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa
     9781 agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg aaagaagaaa
     9841 ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc taaaatctga
     9901 taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa gaaaggaaag
     9961 ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa gtttttattt
    10021 ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag atattcacgc
    10081 atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc gtctaaataa
    10141 gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt ttcatggatt
    10201 tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc agcatattct
    10261 gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa aatgttcatg
    10321 gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt tggtggtgta
    10381 atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt agaattatat
    10441 ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata ctttttgata
    10501 aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa aaaggaattg
    10561 gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc gtatttggta
    10621 ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg gtactcgtag
    10681 gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa cgtccagatt
    10741 taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct ggttttatca
    10801 gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca acattgttga
    10861 tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg gcatcgatcg
    10921 tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga gtcgaaaatg
    10981 ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt ttatttattg
    11041 tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct agcgacatta
    11101 tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt tgcatcatgg
    11161 tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca cgagcaaaaa
    11221 aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt attatttacg
    11281 tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa acgatcaagg
    11341 cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga gcagtcgtga
    11401 tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg atcttaccga
    11461 ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg ggaaaactca
    11521 acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg aatgtctttt
    11581 tactttcatt attattcact gacagtgctt ataactttgc ttacacactt ggtacagcag
    11641 ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct tatcagcgga
    11701 aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta tgggctatgc
    11761 tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca ggcatttatt
    11821 tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac ttagataaga
    11881 ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt gccactggga
    11941 tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct gtcgtttatt
    12001 caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct ttttcagaat
    12061 aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt cacagcctct
    12121 tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa tgctaagaac
    12181 aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg agtcaattgc
    12241 ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat cggattccat
    12301 ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat tgacgaagca
    12361 aaataaaaga cgattcctgc tgagatggat gcgccggaac tattgataaa gcaagcaatc
    12421 atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt ataagtgaac
    12481 aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat cgaagggaaa
    12541 agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata aacagaataa
    12601 ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt tcgataaagg
    12661 agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc agctgcaatc
    12721 atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc aactggatca
    12781 atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac aactaataga
    12841 acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg ttttacaagc
    12901 aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga gaagattcca
    12961 aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac aatagctgga
    13021 tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca ggatcttcca
    13081 aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt gtagagataa
    13141 cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg atgatctttc
    13201 cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt aagtgactgg
    13261 agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc acatctcttg
    13321 ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac ggatggttca
    13381 gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat ttcgcttttt
    13441 tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca gatacatcgt
    13501 tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag atacccgggt
    13561 attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa atctcatttt
    13621 caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct ttcatgatcg
    13681 ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg gataagctaa
    13741 aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct tggaccgaaa
    13801 gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac ctgatgagtg
    13861 gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat tgaatattaa
    13921 ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg ccgctagtaa
    13981 tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg gaaagattca
    14041 gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca atccctgtcc
    14101 agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa aagtaaaaac
    14161 tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa ccatcactcc
    14221 atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat tccatccatg
    14281 ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa atcatccctt
    14341 caaagaccta atggaatgat catcctcttt atcctacccg gtggcagatt gtatatcttt
    14401 ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta gtaaagtgta
    14461 ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc aatttcatag
    14521 aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt atttctatat
    14581 attatcgggc aataaattaa aaaaagcttt accttacaat aagtaagata atttttgtga
    14641 taaaaaaata acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa caactatatt
    14701 ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg aaaatatatg
    14761 gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc atttaaatta
    14821 gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg cggtcttggc
    14881 attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg gatggcgatt
    14941 tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa agaatcagcg
    15001 gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga tatttatggg
    15061 atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc tggatttgtt
    15121 cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt agaagtccat
    15181 cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc
    15241 tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc aacactgaaa
    15301 gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc agtggctatc
    15361 acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa
    15421 tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata ttttattagg
    15481 cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt acactggagt
    15541 aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt tctggacggt
    15601 cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg gtggcttgat
    15661 ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa gccggcttcc
    15721 atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt ttattccagc
    15781 tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag gtgctttagt
    15841 cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt ctcgagataa
    15901 tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag ggccgttaag
    15961 catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg gagttgggac
    16021 agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca tcggcatcat
    16081 catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat ttgcggccat
    16141 caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag cgaatccagc
    16201 gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga caccgatggc
    16261 tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat acggagtgat
    16321 caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt atatgattct
    16381 atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc ttctaacatt
    16441 gtagtggaac actatttttt tataagaata tattaagttt gatctgttat gttatttaaa
    16501 ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga taaccatatg
    16561 ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt ttgatccatt
    16621 tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag atgaaatcag
    16681 cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt
    16741 caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg
    16801 gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga
    16861 taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag ccgcttactt
    16921 tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc taccagcgta
    16981 catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt tgcttcattt
    17041 gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat tctcacccga
    17101 tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc
    17161 tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg gaaatgccgg
    17221 aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt tagtagccag
    17281 ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg tctttgaaga
    17341 atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac aatacacaag
    17401 cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa
    17461 gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag ctgcaacaga
    17521 ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa gccagcaaaa
    17581 attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat cgccaaaaac
    17641 cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg tctctccgac
    17701 agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag acgaattatt
    17761 atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat actgttttgt
    17821 cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat tagctcagcg
    17881 gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc aagcattgga
    17941 aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc gacagataaa
    18001 aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag aattcttgat
    18061 caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag aagtgccagt
    18121 acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa aacgtcaacg
    18181 aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat tcatccaacc
    18241 aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag aagggttctt
    18301 cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga
    18361 agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat ggcaactagt
    18421 agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga aaggaaaaca
    18481 ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt tagaagaatc
    18541 actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa aaacatacta
    18601 agattagagg ggaacatcat tgacgaatga tgttctcctc tattttctat ttttagatta
    18661 tagtgaagag tgaaagaaag ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa
    18721 ccgaacttct ttactgtcat tttagaatca ggtttacgta tgttggggcc atgagcccgt
    18781 gtataggaaa ttggaatcga aatgaagcag taaagttctt tcaaaactta aaaaggagga
    18841 aaacaaatgc cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta
    18901 tacaaagacg acacctgctt atttgaagac gagattgaac acactcaaag aggatttcat
    18961 gcattactcg aagagatcca gcacttgcct gagcgtccat tgattgtttt tgaagcaacg
    19021 gggatttatt ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg
    19081 ttaaacccgt tagaggcgaa aaaacagatg gaagaaagca ccttacgcag ttggaaaaca
    19141 gataaatcgg atgcccaccg gctcgcacag actcatctta aaaatcaacg tcagccaaaa
    19201 gaagtacaaa gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt
    19261 gagaagaaaa tcactcgtct gcgcatggat ttacacaact gccttcaatt gacttttcca
    19321 gagcttgaac agttcttttc aaacaggctg accccttacg cattgacatt gatccgtctt
    19381 tttccccatc cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc
    19441 aacgaaacac ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc
    19501 cactatgcac agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg
    19561 atctattacg ccgaacttct ccaagattta ctcgaacaaa aaaaagcact ggcgacacaa
    19621 atgatcaaaa aagccgaagg ctctccttgc tttttgttgt accaaacgtt ccctgggatt
    19681 ggcgcactca cagccgcttt attgctcggt gaattaggag atatcacacg atttaaaacc
    19741 cataaacaat tgaatgcctt tgtcggaatc gacatccgac ggtatcattc aggtaagtat
    19801 actggccaag accgcatcaa taagcgtggt aatcccaaag cacggaaaat tattttcttc
    19861 accattcgaa atatgattcg ccagcaacgc gcagctccta atcatattgt cgattattac
    19921 tacaaattaa aaaagcaacc tatccctaag aaagataagg ttgccacagt tgcttgtatg
    19981 aacaagcttc tgaaatgtat gcacgctatg gttagggcac atacagagta cgactatgcg
    20041 tacgcggtct cagtggacca ttaactaaac cacttacagt ataccacttc tttgcccaga
    20101 tcgaaaattt tttacgaacg gggctttatt tggtgtgctt tgattttgtc gctttcgttt
    20161 acgagctatt tttcttgcac cttttacttg actaatcgta ggaaatagag gctgggacag
    20221 aagcgtttaa ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
    20281 aatttcagct tatttccgaa ggagttgctt ctgattccgc cgtttatacg tgtttagagc
    20341 gtgagacaaa agtgttcttt acttttgtcc cacgctcagt ttttttatgc gtgttgttgt
    20401 gcgttgtagt attcttcttc cattttttgc atacgttcac gttctttttg gttataccaa
    20461 ggtgaaacgg taaatgcaag gacatcattc agcaagtaaa cagagctgtt cacgaacatc
    20521 gccaaacttg cagatccatg cgtaaatgaa atataccaaa ggaccatttg agcaagccct
    20581 gaagctaacc accagtaata ttggttgttg taacgcaaga aactgatgat accagcagtc
    20641 aagctaatag aaaaagcaat ggcatctacc cacggccgag gatcatctgt ataacgacca
    20701 atcaggaatc ctgaaacaag atagacaagg aaagtactga tgattgcgat aaaccagctt
    20761 tttccagtga attttcggat ttttgaagcc atattatgat tccattcttt acttaacaat
    20821 acggggatat ccaatgtgat catataagca atctgttcac cgatacttaa atagttcttt
    20881 gctgaaaacc caacgataat aaaacaaatc gcagacaaaa tacctaaaat accgttgact
    20941 gattttgccg cattgatgga taaaatacac aatacaccaa gcgtggtacc gataaatgta
    21001 ataacggaca gccaagtgat gggactattg acaagagtca tgacctggca gccaagactg
    21061 aaaaagaaaa gataataatt ttgttgcggc cagcctttta gctgatgagc taaaaactga
    21121 aaatatgact tcatgtatga ggactcctta agacactaaa tgtagtgtgt tctgtattta
    21181 ctaacacaat ctattgtatg cttttcttga aaaaaaacta
//
LOCUS       DAZKAO010000040        21039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000040 DAZKAO010000000
VERSION     DAZKAO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.040"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1969)
                     /locus_tag="ACG8H8_001228"
     CDS             complement(<1..1969)
                     /locus_tag="ACG8H8_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP8986314.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSY"
     gene            complement(1982..2257)
                     /locus_tag="ACG8H8_001229"
     CDS             complement(1982..2257)
                     /locus_tag="ACG8H8_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986315.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2619..3074)
                     /locus_tag="ACG8H8_001230"
     CDS             complement(2619..3074)
                     /locus_tag="ACG8H8_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986316.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3228..4436)
                     /locus_tag="ACG8H8_001231"
     CDS             complement(3228..4436)
                     /locus_tag="ACG8H8_001231"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP8986317.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4548..4880)
                     /locus_tag="ACG8H8_001232"
     CDS             complement(4548..4880)
                     /locus_tag="ACG8H8_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP8986318.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5028..5903
                     /locus_tag="ACG8H8_001233"
     CDS             5028..5903
                     /locus_tag="ACG8H8_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8986319.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(5985..6566)
                     /locus_tag="ACG8H8_001234"
     CDS             complement(5985..6566)
                     /locus_tag="ACG8H8_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP8986320.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6572..7831)
                     /locus_tag="ACG8H8_001235"
     CDS             complement(6572..7831)
                     /locus_tag="ACG8H8_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP8986321.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7833..8879)
                     /locus_tag="ACG8H8_001236"
     CDS             complement(7833..8879)
                     /locus_tag="ACG8H8_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986322.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9114..9389)
                     /locus_tag="ACG8H8_001237"
     CDS             complement(9114..9389)
                     /locus_tag="ACG8H8_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP8986323.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9632..10942)
                     /gene="der"
                     /locus_tag="ACG8H8_001238"
     CDS             complement(9632..10942)
                     /gene="der"
                     /locus_tag="ACG8H8_001238"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP8986324.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11124..12353)
                     /gene="rpsA"
                     /locus_tag="ACG8H8_001239"
     CDS             complement(11124..12353)
                     /gene="rpsA"
                     /locus_tag="ACG8H8_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP8986325.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12488..13168)
                     /gene="cmk"
                     /locus_tag="ACG8H8_001240"
     CDS             complement(12488..13168)
                     /gene="cmk"
                     /locus_tag="ACG8H8_001240"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP8986326.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13217..13852)
                     /locus_tag="ACG8H8_001241"
     CDS             complement(13217..13852)
                     /locus_tag="ACG8H8_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP8986327.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(13918..15321)
                     /locus_tag="ACG8H8_001242"
     CDS             complement(13918..15321)
                     /locus_tag="ACG8H8_001242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP8986328.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(15308..16339)
                     /locus_tag="ACG8H8_001243"
     CDS             complement(15308..16339)
                     /locus_tag="ACG8H8_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986329.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16370..16591
                     /locus_tag="ACG8H8_001244"
     CDS             16370..16591
                     /locus_tag="ACG8H8_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP8986330.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16756..17493)
                     /locus_tag="ACG8H8_001245"
     CDS             complement(16756..17493)
                     /locus_tag="ACG8H8_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP8986331.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17646..18986)
                     /locus_tag="ACG8H8_001246"
     CDS             complement(17646..18986)
                     /locus_tag="ACG8H8_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8986332.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19223..20746)
                     /locus_tag="ACG8H8_001247"
     CDS             complement(19223..20746)
                     /locus_tag="ACG8H8_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP8986333.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         6046 a         4492 c         3513 g         6988 t
ORIGIN      
        1 gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac ttcgtattca tcaatgattt
       61 ttctttcaag ataataacct cttagcattt ttttcgtaaa cagactatct tgctggatca
      121 tccacgaaga aatcaaattc cccgagttca tttttattcc ttcaagtttg cgttgctgga
      181 taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa gttatctaaa ctttcaataa
      241 tcaactgagt atttttttga tatgtctctc tgatttcttg gatatcttca tcactaataa
      301 atgtctgacg atcagcatat cttctttgcc aaaacatttt cggatggaga acgattcgca
      361 ttaacctacg gacaacaatg aaccaaaagc cgataaaaga caaaatctgg cttgtgatag
      421 aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata accattagaa gaaagcttgc
      481 ccgaacgata actttcatcc aatccatccc gttcgatcga caaaatcagt gcttgtattt
      541 cttgtacttc ttgcttttca tcatcggtta aatcttccaa gtacagatca cgtatccgtt
      601 cttgataatt ttctatcact gcttcagtgg ctaaaaattc ttttgtatta cgatcaattt
      661 cctttatttc ttttcgcaaa acttcaacga cctcttctaa aatcatcaaa gcattcaaat
      721 ccgtgctttt cgcttcctct gtttccgtca gcataggcaa gacaatgatc cctatgacca
      781 aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa agaccgctgg taaaacatca
      841 tattattgat tgaaaatggc aaaataaaaa tcgtagccaa actgactgtt ccctttacac
      901 cgccaaatgt taagatcaag atctcgttca actgtttttt gaacttttta ctgccatctt
      961 tgaatacata aaaaagactg atagaaatga atctgatcac aaaaagcatg acagagatta
     1021 atacgataat cgctaataaa agaccgtttg gatataaacc gtctccccat actggcgaga
     1081 acacttgcgt caattcgatt cctaaaaata taaatactaa tgcattcagt gtaaaagcaa
     1141 tagttgtcca tgtactgtgg gacaagctcg acaattctgc atcaaacaca gagattttcc
     1201 taaaaccaga agcttggaga atacctgctg ccactgcagc aatgattcca gatacatcaa
     1261 atgcctctgc caatacatag gctagaaaag gaagcaaaag ttcgatcagc agataccctg
     1321 tgacatcttg tgctgatgct ttttcgatca aatgaattat tttttgtttg aaccagacaa
     1381 gcaaaaagcc gatcaaagct cccccaatac tagaaactac tagactcatc cccgcgttta
     1441 ctgctgaaaa agatccggtg atcagcgcac caagagcaaa ctggaaggca gtaacaccag
     1501 atgcatcatt caaaagacct tctccttcca aaatatgcat cgccttttta gggatattca
     1561 ctcgtccaga aagagaactg actgctaccg catctgtcgg acctaacgct gctccaaaag
     1621 caaagcaggc agcaagtggg acacttggca acaggaatga taacgtcgca cctaccccga
     1681 caagggtcaa aatgactcct ccaaatgcca agaacaaaat tgtcccgaaa tttttgagaa
     1741 tcgaagaaat atctgctttt tctccttctc taaataacaa tggtgcaata atcataacta
     1801 agaacaattc aggttcaaaa tcgattgatt gtccccactc tgtcagacct aataccactc
     1861 caaggaaaat ttggatcagt ggagctggaa tcattggcaa aatgcggcta gctatgttcg
     1921 ataaggtgat cgttactgca aatacaataa ctaactcagc aaattccatc gtccatcccc
     1981 tttatttttt attttgtgta agcattttta aaaattgttc ttttttcttt aaaattttgg
     2041 tttcgactac ttcaacctgt gtttggttcc cctctaattc acactgtaca cgttttgact
     2101 ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg atacatcaat atatttcttt
     2161 tacgcaactt acaaagtatt tgacggcggt catccatcga aagggcgcca tagcgatcat
     2221 gcaacagttt tttttgttgt tcaaaactct ctctcatttt ccagatctcc tatacacaaa
     2281 cttccatcat tatagcaaag ttctttttgg atgaaaaata tttcattgtt tcatcgttta
     2341 ttgctagtta gaaatttcca taaatttttc aacgcctcat tctattccaa atagtatcag
     2401 atatcaagta aaacttaaga aagataaaag tttctgacaa aaggatgttt actttcccat
     2461 tcctgccttt cttgttcttt tatcacaaca aaaaaatggg gctgcgacaa aaatcttgtc
     2521 acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag taataagaca
     2581 catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa ttccccgtat
     2641 acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt tttttcgctg
     2701 atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag atatgggaag
     2761 tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat atcttcgtat
     2821 ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc atgtgtcgca
     2881 actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg gatttcaaca
     2941 aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca aagatcgccg
     3001 atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt tagcgctgtc
     3061 atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta ctacctcagt
     3121 ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg acgtatctgc
     3181 ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta aatcaatttt
     3241 tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg ttctacgacg
     3301 gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt tccagtttct
     3361 tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg taaacgatca
     3421 taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat caattcttct
     3481 acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa agctccagtt
     3541 tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg gttagaacac
     3601 ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg gctgatcaat
     3661 aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc aaaacgtaaa
     3721 agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt ttcaataaat
     3781 gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc cacatttata
     3841 cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta gaaggtttct
     3901 tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat tcgtaaggca
     3961 tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc ttctagatct
     4021 tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa cgcattgatc
     4081 gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac atgatctggt
     4141 ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata ttgttcatca
     4201 ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt tggaaaaagc
     4261 tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat tggctggttc
     4321 agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc attctctaat
     4381 tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa tttcattttt
     4441 ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg gtgactgttt
     4501 gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta ctcgtcctta
     4561 cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc aaaaacttca
     4621 gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac atcaccaagt
     4681 tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc ataagtgtgg
     4741 ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg agctaatgga
     4801 gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg catactgtat
     4861 aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa ctttaacgca
     4921 aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag gaaggaattt
     4981 ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg gtcaaacgaa
     5041 aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca tttggtcttg
     5101 tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc accttgatct
     5161 taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac atcccattga
     5221 ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt ctaggtacac
     5281 tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc aatttacagc
     5341 atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga agcgggatcg
     5401 tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt ttagaacaaa
     5461 attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc ttacttgctt
     5521 cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg tatgttttca
     5581 gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta cttgtcgtct
     5641 ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga ggtgtgactt
     5701 tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat atggtcgtta
     5761 gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa aaagctttcc
     5821 tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg aagccacaga
     5881 caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg aaagattcca
     5941 catccagctg acctttgtct tttttagtac tatactattt ttaactaatt gttatgttca
     6001 cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac ttcatcagat
     6061 aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg agcataaagc
     6121 atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa ttcagtatct
     6181 tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat agatagatat
     6241 tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt tttccaattc
     6301 aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg caaaaacaat
     6361 ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc cgcaaaattc
     6421 gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag ataattcaag
     6481 atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt taaaaaattc
     6541 ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca tctaataatg
     6601 acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat tcttgcgctc
     6661 ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa tctggttcat
     6721 gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag tcttctaatt
     6781 cattataagc atgggctagg ttccattctg cataaggatt agcagtttct tccatctgat
     6841 tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta cttaaagtaa
     6901 gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt tcttctgctc
     6961 gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt tcgacttggt
     7021 aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc atttcttctt
     7081 cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta acttctcgaa
     7141 gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc gtaaaagcta
     7201 gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa ggtacgatag
     7261 ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc atcgagattc
     7321 ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct gcctctttga
     7381 aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt tcatcaggaa
     7441 gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg ataacttggt
     7501 acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta tccacttttt
     7561 ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct gctaacggga
     7621 tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc tgttttgctt
     7681 cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata tctgctgggt
     7741 cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca tctgataaag
     7801 cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc actgacgatt
     7861 tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg gtatccgtct
     7921 attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa aaaaagaact
     7981 ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata gccaagcaag
     8041 tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg gagattccac
     8101 acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag atggccattt
     8161 tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt ttctgcctct
     8221 aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt cgttaattgc
     8281 tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca aatcattccg
     8341 caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc gctgattgtt
     8401 tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg atttggtagc
     8461 tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga tacagggatc
     8521 atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag atccatgatc
     8581 ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg tttgacgaaa
     8641 tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca aatataccag
     8701 tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa atcataatag
     8761 cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag ctgactgact
     8821 agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc aagttccata
     8881 cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta gaaaagataa
     8941 gagcaaagct cacctcataa cttgcaaata aataaaaatt aaataaaata catgtgtttt
     9001 ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt ctgctaggaa
     9061 ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact gtcttattta
     9121 acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc tggaatttcg
     9181 atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg aacttcaaag
     9241 ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg aatagttgaa
     9301 aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc tgaagcaact
     9361 ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac aagagatatg
     9421 atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa agaaatattg
     9481 attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc aaggttttta
     9541 ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg tctttttctt
     9601 ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg ataattcgga
     9661 tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa cgttcataag
     9721 aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt ttgacagcga
     9781 cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt ggattgattg
     9841 ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga ttctggttca
     9901 tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc actgcagaaa
     9961 caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct tgttcgaaat
    10021 cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg ataataattc
    10081 cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga atcccttctt
    10141 cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt gcacgcatca
    10201 cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca gctgtatcta
    10261 tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg tctcgggtcg
    10321 ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg atcaaagagg
    10381 atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg tcctcttctt
    10441 cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc cctagtccat
    10501 ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca tagatatcgt
    10561 ttctcatttc tggattatct actttattga ctgctaaaat cactggttta ttacttctgt
    10621 ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg ctgactacac
    10681 aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc tgatccataa
    10741 aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct cgacctaacc
    10801 attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg acaatcgaga
    10861 tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg cggccaacga
    10921 ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc tcacttagtt
    10981 taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt atttttattc
    11041 cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag ctgctttcat
    11101 ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt ttcttctact
    11161 tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt ttcttctggt
    11221 aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc ttctaaagct
    11281 ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt cactttcact
    11341 tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga aatttgagag
    11401 atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa gcttgtcaaa
    11461 cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat ttctgaccaa
    11521 ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg attgatagac
    11581 aacactttga catttacatc atcattgaca gccaatacat cacttggttt tgctacatga
    11641 ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc gacaaatgcg
    11701 ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg gatagttgaa
    11761 agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg cgataagatc
    11821 aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt gcctttatag
    11881 tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa accacgtaca
    11941 ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc gatgaccttg
    12001 ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa acggcgtttt
    12061 gaaagtagat agctgccgtt ttctttatct ttgccgatcg tactgataac gactaaatct
    12121 aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt tgataattct
    12181 tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac ttgtttgtct
    12241 tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg tacgcttttc
    12301 attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt cataacactt
    12361 gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa ttgattattc
    12421 aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt tcattttttt
    12481 tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt gctgcaacga
    12541 cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct tgcttcagcg
    12601 gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc gtcagtgttt
    12661 caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct gcacgctcac
    12721 ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct gtcccgatgt
    12781 ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca gcaactaatt
    12841 cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca tctggttgac
    12901 ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct tcttgttgcg
    12961 aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct gaaaaagata
    13021 cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta tctgtataaa
    13081 tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat gaagcaggcc
    13141 cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga aaaaaagccg
    13201 caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc ataaagttgt
    13261 ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca cgctcagcca
    13321 cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca gctgatgaag
    13381 aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt gcctcacttg
    13441 aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct gtatcttgag
    13501 acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca gttgactcct
    13561 cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa gaagttgctg
    13621 ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct ggaatagcga
    13681 tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct ttcttttgtt
    13741 gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt tgttcccatg
    13801 gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc actgttttac
    13861 ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc taaaaaatca
    13921 atgttccatg ttttttttaa ataaatttag taaaatcgtt ccccaatgaa gttgttcagt
    13981 cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa tatcacaaca
    14041 attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt ttctgcggca
    14101 gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt ctttttcctg
    14161 acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca gctccatcca
    14221 ttttttttgc agctcatcaa agggagcttg ctctgtggca tattccaaat aaagctcaaa
    14281 actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt catccccact
    14341 ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac cgatttcttg
    14401 tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga tatcagattt
    14461 atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat tttttacaaa
    14521 ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt atccaactgt
    14581 aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg cacaataaat
    14641 gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt tttgctgcgt
    14701 tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct cttttggtgg
    14761 tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg tcgccgttag
    14821 tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt gatattctgg
    14881 acggaaatca actccccatt gagacacgca atgagcctca tctactacat ataacgcaat
    14941 attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg gactcaaaaa
    15001 caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt gcgaaagtaa
    15061 actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca cttgatcttc
    15121 cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat accccgtcaa
    15181 ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat cttctccatc
    15241 aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc caaaatattt
    15301 ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag atttgataca
    15361 aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag cgccactctc
    15421 gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga atccactcgt
    15481 atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact gttcctcttt
    15541 ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac atctgcttgg
    15601 tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa gcgtgccaaa
    15661 gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg ctttgttgca
    15721 gataaaccgc tgactcatca ttgttttttg ccagctgata aggaagcagc cctgttcgct
    15781 catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc tccgaaaaca
    15841 gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat tggctttcac
    15901 taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga agatagtctt
    15961 gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc cgccaactag
    16021 tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt tgatgttcgg
    16081 acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt tcgatccaac
    16141 cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat tctggaaaac
    16201 tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa gtcgttcgtt
    16261 ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat gaaaatagcg
    16321 ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa tgtcactact
    16381 atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg aagcgccaga
    16441 tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag aggctcatca
    16501 acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa aatgtccagt
    16561 tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa aacgtacact
    16621 aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg aaatcccgac
    16681 ttattttgaa agattcattt gctatctata gttaattgac tagttttttc acggacccta
    16741 taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat tgacgatcac
    16801 agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct caccatagct
    16861 gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt ccggtaaata
    16921 ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg ccgtgatcga
    16981 taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac ttgccatgaa
    17041 gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac ttgccattac
    17101 gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt ctttgacctt
    17161 atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa tagcgactga
    17221 aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct ctacgagagc
    17281 accaggataa aaaataagta atggagactt tccatgattc tttttctctc cttcaaaaaa
    17341 taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg acgatggttc
    17401 gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta aaatgatgac
    17461 taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct atctgagtga
    17521 cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa atgcaaactt
    17581 tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt tttgtatgtt
    17641 tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat tgctttatac
    17701 atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt atcagggaac
    17761 atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc gcctgataca
    17821 aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt tttcactaac
    17881 tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata agtcaaccca
    17941 tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa tgtaacaggg
    18001 attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact tgcaaagttc
    18061 atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc accagttgca
    18121 ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac ttctgaaaga
    18181 gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc gtggatactt
    18241 ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg ttttttcaat
    18301 gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg aacaacattc
    18361 aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa gtaagcttgc
    18421 aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa tccttgcgta
    18481 attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc ttcatctgta
    18541 ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa agctttgacg
    18601 gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc aactaaccag
    18661 ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc ccctgctgga
    18721 acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat ctctgaataa
    18781 ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact gatttccaat
    18841 actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc tgaatctgct
    18901 tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc ttgttcatga
    18961 tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa aaagggtcat
    19021 gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag cctgctccat
    19081 cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga atggagctat
    19141 tgacttatcc acgtgcttat cgatagccgt ttatcggttt cttcattctt atgatagacc
    19201 ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc acacagatgg
    19261 ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa acatcgaaac
    19321 gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag tttactaaac
    19381 cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca aattcaccca
    19441 taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga atcaagaatg
    19501 acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat aatgaactta
    19561 atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc ataaccactt
    19621 ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa gcaccagcta
    19681 aaaattcaga gacaaattca gactctttca tgccataagt gattgctact acgatggaca
    19741 ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga agcatgcttt
    19801 tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat gattcaaaaa
    19861 tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg ataccaaaag
    19921 taataaagaa caatacatta acatgttttt gttttttcgt gattacttct tgacgaccga
    19981 ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact aatgattttg
    20041 ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa atagctaaca
    20101 aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc ccaacagctt
    20161 gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct acttgagacc
    20221 ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc atgactggga
    20281 tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag ccgcctaatg
    20341 caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat ttcttcacaa
    20401 ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa aatccaccaa
    20461 caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt agctcccccg
    20521 ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc ccttgaggat
    20581 tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct acactatagt
    20641 gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata aataagaccg
    20701 tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc ttcttccttt
    20761 cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa gagtatcaga
    20821 acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt tttccctatt
    20881 ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg tttccttttt
    20941 tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg caaagaattg
    21001 attgattttt atgcataaat caaaaaaata ctcaaaaca
//
LOCUS       DAZKAO010000041        20918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000041 DAZKAO010000000
VERSION     DAZKAO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.041"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..492)
                     /locus_tag="ACG8H8_001248"
     CDS             complement(19..492)
                     /locus_tag="ACG8H8_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8986334.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(597..1460)
                     /locus_tag="ACG8H8_001249"
     CDS             complement(597..1460)
                     /locus_tag="ACG8H8_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP8986335.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1656..3755)
                     /locus_tag="ACG8H8_001250"
     CDS             complement(1656..3755)
                     /locus_tag="ACG8H8_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP8986336.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3975..5084)
                     /gene="rpoD"
                     /locus_tag="ACG8H8_001251"
     CDS             complement(3975..5084)
                     /gene="rpoD"
                     /locus_tag="ACG8H8_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP8986337.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5108..6949)
                     /gene="dnaG"
                     /locus_tag="ACG8H8_001252"
     CDS             complement(5108..6949)
                     /gene="dnaG"
                     /locus_tag="ACG8H8_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP8986338.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7131..9446)
                     /locus_tag="ACG8H8_001253"
     CDS             complement(7131..9446)
                     /locus_tag="ACG8H8_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8986339.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9631..10491)
                     /locus_tag="ACG8H8_001254"
     CDS             complement(9631..10491)
                     /locus_tag="ACG8H8_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP8986340.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10550..11182)
                     /locus_tag="ACG8H8_001255"
                     /pseudo
     CDS             complement(10550..11182)
                     /locus_tag="ACG8H8_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11349..12110
                     /locus_tag="ACG8H8_001256"
     CDS             11349..12110
                     /locus_tag="ACG8H8_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8986341.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12136..14727
                     /gene="mprF"
                     /locus_tag="ACG8H8_001257"
     CDS             12136..14727
                     /gene="mprF"
                     /locus_tag="ACG8H8_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP8986342.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14915..16102)
                     /gene="tuf"
                     /locus_tag="ACG8H8_001258"
     CDS             complement(14915..16102)
                     /gene="tuf"
                     /locus_tag="ACG8H8_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP8986343.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16470..16826
                     /locus_tag="ACG8H8_001259"
     CDS             16470..16826
                     /locus_tag="ACG8H8_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8986344.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17067..18071)
                     /locus_tag="ACG8H8_001260"
     CDS             complement(17067..18071)
                     /locus_tag="ACG8H8_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP8986345.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18237..19088
                     /locus_tag="ACG8H8_001261"
     CDS             18237..19088
                     /locus_tag="ACG8H8_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8986346.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19105..19350
                     /locus_tag="ACG8H8_001262"
     CDS             19105..19350
                     /locus_tag="ACG8H8_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986347.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19537..20241
                     /locus_tag="ACG8H8_001263"
     CDS             19537..20241
                     /locus_tag="ACG8H8_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986348.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            20649..>20918
                     /locus_tag="ACG8H8_001264"
     CDS             20649..>20918
                     /locus_tag="ACG8H8_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8986349.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCG"
BASE COUNT         5943 a         4277 c         3662 g         7036 t
ORIGIN      
        1 aaaaagccgt ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg
       61 acagattcca tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac
      121 tacttgctcc acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa
      181 gcatagtaaa tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa
      241 tccatcatta aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg
      301 ataaacagta gcaagtccaa tttccggact cttttttttc actaaaaaga agatttcttc
      361 tgcagataaa tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg
      421 tgtcagctta aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc
      481 attcatgacc atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata
      541 acactatgtt aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa
      601 ttagattttt tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa
      661 agattaccta atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct
      721 tctggatccg atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt
      781 aaaatcattt gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc
      841 agtacaccgt ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt
      901 tcattatatc gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac
      961 gtacctgtta atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct
     1021 gttccatatt tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg
     1081 tcttttgcat caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct
     1141 gtcattgtcg gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg
     1201 acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata
     1261 cggcttttag gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat
     1321 ccttcaactg cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt
     1381 ccgttttttt ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata
     1441 gttgctaata aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt
     1501 tctttgctat cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt
     1561 gaaaaaagga gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa
     1621 acggcaggct cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac
     1681 catccagttt tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct
     1741 ttcatcaatg cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg
     1801 attctggctt tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta
     1861 gcaatcactt tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca
     1921 atatcacttg ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc
     1981 gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct
     2041 gcagctgctg cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct
     2101 gcttttttct tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt
     2161 ttcaattgtt cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct
     2221 tttgccgtct tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact
     2281 atagctgttt tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt
     2341 gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta
     2401 tctgctttca acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac
     2461 cactcattca ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct
     2521 aactctgaat atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa
     2581 tcttgatgaa tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca
     2641 agtttctcat gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct
     2701 tctacttttt gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct
     2761 gctaaaacga gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt
     2821 ttttctaagt ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc
     2881 gcttctgctt caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca
     2941 gtaccctctt tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc
     3001 tctgttgact ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta
     3061 ttcgtttttt ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc
     3121 gtttttacgt cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct
     3181 ttttcagttt cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg
     3241 atcattttga gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata
     3301 taagcggata gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact
     3361 cttctttttt gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc
     3421 tcatgaacag ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca
     3481 ggtggttctt cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt
     3541 aaatctttca attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca
     3601 tcttcagttg atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt
     3661 atttgctgat cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc
     3721 atttttttcg atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta
     3781 ggtttacgct accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag
     3841 gatttactta aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg
     3901 tagtctcaag ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg
     3961 tcaatagtcc acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc
     4021 aattttctta atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact
     4081 ttccctactt cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg
     4141 acattctctt cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac
     4201 tcataagctg cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag
     4261 tgagagtcat cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc
     4321 aagatttctc ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta
     4381 ggttctcttc caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt
     4441 tcaaccatat gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg
     4501 gcttgacgaa tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat
     4561 ttttcaactg ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg
     4621 cctcgtccta cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga
     4681 cgctgtttcg cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc
     4741 gcagttaaaa gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt
     4801 tttacaccgg ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca
     4861 ctcttcaagc tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct
     4921 tcaatctttt ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc
     4981 tgattggata aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct
     5041 gctactgctg cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct
     5101 tgagtagcta tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt
     5161 caagctcaag ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg
     5221 ttatttcatc agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg
     5281 gcgaactttc ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt
     5341 catcttgcaa aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg
     5401 catcgaataa aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt
     5461 ttaaaatatt acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct
     5521 tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt
     5581 gttgactttc ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt
     5641 gtttttgtat cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat
     5701 cttgttcgat tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga
     5761 tcttgtcttt ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa
     5821 agactgtttc gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga
     5881 gatattcatc aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt
     5941 tttgccgaag caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat
     6001 agcaaaagac caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac
     6061 ttgtccccat agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca
     6121 taaacccttc gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa
     6181 gattaaataa aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg
     6241 gctgatcttt ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct
     6301 ggccttgaga attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc
     6361 catcatcatg cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat
     6421 tcttgaatac ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt
     6481 caattaattc taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt
     6541 ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt
     6601 tccccgtagc agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta
     6661 acggtatctg ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta
     6721 gttcttgaag aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct
     6781 gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca
     6841 agtaattttt accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg
     6901 tttgactgcg tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc
     6961 ctgctctatt gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa
     7021 aatcgcacta actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta
     7081 tcatatttca ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct
     7141 actcttttca gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata
     7201 tttacgagca tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc
     7261 aaaattccat ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg
     7321 aagatgcgca atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca
     7381 atcagcaagt aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca
     7441 agtagataca ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata
     7501 agtgctgtag gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag
     7561 aaggaaggat acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga
     7621 aatccaattg ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata
     7681 aaaaagatgc ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact
     7741 tctggcaatg tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct
     7801 ccatcacctt ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc
     7861 acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat
     7921 ttcttttgct gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact
     7981 tccatttctt cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca
     8041 cgtctagcga tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca
     8101 ccttcctcat gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt
     8161 tcaaacagag caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga
     8221 gcgatcccca acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga
     8281 agccgatctt cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga
     8341 aattccatcg cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac
     8401 cgattagaaa gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt
     8461 tctgccagat acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct
     8521 tctacccgct ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat
     8581 gtatcgacat gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt
     8641 gccagtttgg ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga
     8701 agcatgccta acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg
     8761 atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat
     8821 ttggatactt tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc
     8881 tcgctagtca agcggacagc ataattatct gcacctactc ggataacttc agccatgaca
     8941 gaaccgcttg tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa
     9001 tcagattcgc ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa
     9061 tcggctggta tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta
     9121 gtagaaatcg attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc
     9181 gataatttcc gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg
     9241 acgacaataa ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc
     9301 ccaataccaa agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt
     9361 gtactttttg atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact
     9421 acctcttgct gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa
     9481 ataatcacgt gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat
     9541 ttatagaaaa acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac
     9601 ccgtgtgctg tttttattat caatctatct ttacttatat tgttcctcca aacggctcat
     9661 ccaccaacct agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac
     9721 tcccaatccc gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa
     9781 aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa
     9841 tccaagaaca caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc
     9901 tttaaatccg tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct
     9961 caatcctggg acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa
    10021 attctgatcg acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag
    10081 aacaaaagta acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc
    10141 tttccataac gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt
    10201 ttcatacgca cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc
    10261 gcctagacca acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc
    10321 taaaaaagaa atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac
    10381 tcctggcaag ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc
    10441 tttggttgtg taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgt
    10501 actccttctt tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga
    10561 ctttttcata ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt
    10621 tacctccatt atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc
    10681 ggtattgttt cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt
    10741 ctgctaattc tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc
    10801 caaaattata tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta
    10861 tcatttccgc caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct
    10921 cagcttctcc atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt
    10981 cggtcaatat tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag
    11041 ctgttgttat ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc
    11101 ataaaaacaa caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa
    11161 tagataatcg ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta
    11221 ctatttcttt atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa
    11281 tgaattttcc tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca
    11341 attgcccaat gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag
    11401 aaaagaaata tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag
    11461 gcttccctct ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg
    11521 tccctgtact cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt
    11581 cgacaaacga agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca
    11641 tgttgctgga aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag
    11701 cactggtatt tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa
    11761 acacgcttgt taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat
    11821 gggtatggtt actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac
    11881 ttttgctgca tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt
    11941 tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa
    12001 ctataccaaa agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag
    12061 atcctgaacg cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa
    12121 tagaaggtga tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg
    12181 ttcaaaacaa tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc
    12241 ggtaaaactc tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa
    12301 agcggactaa tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt
    12361 attttgaatc ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg
    12421 ctgatcaata cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc
    12481 tctgaactat atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt
    12541 ttcttgtttt tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc
    12601 ttcacgccag ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta
    12661 tattttcctg tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc
    12721 tcacttaaaa ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg
    12781 tttagtttcc tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt
    12841 ccattattca ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg
    12901 agtttcgatg taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc
    12961 gttgtgtgga tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc
    13021 gtctttttct tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa
    13081 caattagcaa cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc
    13141 atgctagtac tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt
    13201 ttgaacccat tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt
    13261 ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc
    13321 ttcttgattg ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg
    13381 gttttcttgt ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa
    13441 caattgatct attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc
    13501 ttgctttaca tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat
    13561 tttatttcct ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc
    13621 atcgctgtta gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag
    13681 attggcgaag cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac
    13741 tccgatagtc aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt
    13801 gaaccaactg ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat
    13861 ccttctggta agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat
    13921 cgactatgct atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat
    13981 gaattcggct acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt
    14041 acaacttcag ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa
    14101 ggatttacat ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa
    14161 aatatctctg ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc
    14221 tctgaagatt atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt
    14281 gtcgcttttg caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg
    14341 atgcgatatg attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg
    14401 tttgcctata tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca
    14461 aatgtcggaa cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag
    14521 tttggttctc atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca
    14581 aactgggtct cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt
    14641 gctttgctga ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg
    14701 aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga
    14761 tgaataaaag agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa
    14821 ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac
    14881 atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac
    14941 ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga
    15001 ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat
    15061 cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat
    15121 agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg
    15181 ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga
    15241 tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat
    15301 ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga
    15361 tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg
    15421 caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat
    15481 ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag
    15541 cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag
    15601 tgtcatcgcc tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat
    15661 cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta
    15721 caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg
    15781 cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat
    15841 agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta
    15901 cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag
    15961 catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta
    16021 aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc
    16081 tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa
    16141 atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc
    16201 gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa
    16261 ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg
    16321 ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat
    16381 gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt
    16441 cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt
    16501 taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt
    16561 attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga
    16621 aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg
    16681 tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag
    16741 tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa
    16801 atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag
    16861 gctgggacag aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt
    16921 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt
    16981 gttatattcg tcttttccga tccttctctt gacacaattc atttaactga tcactggtac
    17041 ttgaacacct aatattctag tcatttttat aaacgattca atacttcatc cacatttttg
    17101 atcatcacgg atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga
    17161 tatacatcta ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca
    17221 aattttttct cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct
    17281 tgctcttgta cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt
    17341 tccgcaatca tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct
    17401 ttcaaatcag ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct
    17461 actttttgga tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact
    17521 cttccagaaa aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg
    17581 taggtccaat cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct
    17641 agaattgctc gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag
    17701 tgagtgtaag cctcacgata attgactttc aataaagata agtacatcac tgtatctaat
    17761 tcatacaaga caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg
    17821 aaccaccgct gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt
    17881 gattcaacga tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt
    17941 ttttggatct tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt
    18001 tgtgaaaaga ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa
    18061 tagatatcca ttttctcatt cccttctgtc ttagtttacg gaaaaaaaat caaaaatacc
    18121 agaatcaaaa tcattttctt ttaaacaaaa atttcaagag tagaaacaaa atggattctt
    18181 tttttcgaaa aatgcgctac acttatagtt gaacgaataa aaaaggagct gaaagtatga
    18241 attcattaac aagcacttat cgtctatcaa atggttacga aatcccggta gtaggattcg
    18301 gcacatggca aacacctgat ggggatgtcg ctgtttcctc tgttaaagaa gcgctggctg
    18361 caggttatcg ccatatcgat actgcccaag gatacaagaa tgaagaaagc gtcggccaag
    18421 cgattaagga aagtggaatt cctagagaag agatattctt gacaacaaaa ttatggaatg
    18481 caaaccacag ctatgaactt gtgatgtctt cttttgaaga atcattgaag aagctccaaa
    18541 cagattattt agatctgttt ttgatccact ggccaaaccc tgttgctttt cgtgataatt
    18601 gggaacaagc aaatgcagat acatggcgag catttgaaga actgtatgaa gcaggtaaaa
    18661 tcaaagcaat cggtgtgagc aatttcttac cccaccatct agatacacta gcaaaaacag
    18721 caaaaattat gccgatggtc aatcaagttt tccttgcacc aggcgaatta cagacaactg
    18781 tagttgagta tgcaaaaaaa catgatatga tcctcgaagc atatagtcca ttaggcactg
    18841 gtaaaatctt cgatgtgcct gaaatgaaac aaatcgctga agcacatgac aagacgattg
    18901 cacaagttgc tttgcgttgg tcgctgcaac acgaattcct tcctcttcct aagtcagtga
    18961 caccaagcag aatcaaagaa aatactgaat tatttgattt tgaattaaca gaagaagaaa
    19021 tgaaacaaat cgatcagtta gatggtgtag tcggcaaagc aaaagaccca gatactaccc
    19081 aattttaagt cgaaaggtga ttttatggat ccaatcgaac gcttgaattc tctctcggaa
    19141 gaagttaccc agacttttca ttccgatttt gtctttttga tcgatgcaga gaaaatccaa
    19201 cattttcctg caagaaactg gacacatgat caaatcatcg aagaactgaa aaaacggttc
    19261 gatcattcgc taatggtcaa accctggcac gaacatgaag tcatttattc acctgaacta
    19321 cctgtttttg ccttgatacc gaaaaaatag aaagaatgga acggattatg tatatataca
    19381 taatccgttc cattttttat ttttctttca aaaaacggga aaatacgcaa atttttctgt
    19441 tgtaatttgc gtatacgcaa gtttttttgc attactcctt ttcatttggc tgtttttttc
    19501 ttataatcga aagggtatga gcgaaggaga gaacatatga agaaaaaaca gaaacaaaaa
    19561 ttattaaatc aattccgtcc ttcattggat ggtgtaagag gacaattatt ccgttctcta
    19621 gaagaagaga gcttgcgacg ttatggtttg ccgttacaag tcatgcttga tcccaaaaac
    19681 cgtcaaatct taacgatcgg attagctggg aaaacaaatg aagatacacg gatcaacgtg
    19741 ccaatcgatg acaacttcaa tactgtatta aagagaattc gttcaggaga aaacgggatt
    19801 ttcgaacgat tccgtgacaa tcttcttatt gaaatcgtgt cttactggaa tgatcaacga
    19861 ttgaaaaacg atgaaccaac tgcgcaagct gttgcgacac cagttacaga agaaacagtc
    19921 agtgtgccag aagaaaaaac ggaaaaacca aaagcggaca caactgaaaa tacacgctca
    19981 actgatacga atgccgcttt aagttttgct gatttttcaa aagccgttgc tgagtatcct
    20041 aaattttaca cagaacaaaa tgaacatgaa gtagttatta atgaaaaaaa tgctgatgaa
    20101 cctcgtaaat tagcaactat ttctatgact actgaaaatg agttcgttat cgaaaaagca
    20161 ctcgaacgga aatacaaagt caaattgact ttgattcctt tgatcgaaca atttgcagcg
    20221 acagcgatta ctgatcgata aaaattctat ttgtcataaa aggctgggat ttaaaatccc
    20281 agccttttta gtcatataat aatttctaca gcaagtaaaa agaaattcct tctaacatca
    20341 ggctcaaata tcagcttgac ctgtccaaca aatcacctat gatcctacaa tgttaggcga
    20401 cttctcttct acctgaagat atcttggagt tgcctgattt cttaagatgc ctaaccttaa
    20461 ctataaaaaa ttctaataat aaacatattt tctgatagag aaaaaagcgg ttctataata
    20521 ttttgtgttg gtggaaaatc gaaagcgatt ttctactaac acttttttct tcgtttcaac
    20581 tcaaaaagag actcactttt ggtaaaattt agtcgaccaa aacgtcaccc taccaaagga
    20641 gtctctcaat ggataatcat actagaaaat tactcggatt aacagataaa catttatttt
    20701 ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag tttattaaag
    20761 ggagattgga tgactcacct tcccattgtg aacactgtgg atcgattcgt attatccgca
    20821 acggttccta tacgacacgc actcagatgt tgaaagtcaa agaaaagttg actattttag
    20881 aattaaaacg tacccgtttt ctctgccatg attgtgga
//
LOCUS       DAZKAO010000042        20235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000042 DAZKAO010000000
VERSION     DAZKAO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.042"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..2127)
                     /gene="glyS"
                     /locus_tag="ACG8H8_001265"
     CDS             complement(52..2127)
                     /gene="glyS"
                     /locus_tag="ACG8H8_001265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP8986350.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2129..3046)
                     /gene="glyQ"
                     /locus_tag="ACG8H8_001266"
     CDS             complement(2129..3046)
                     /gene="glyQ"
                     /locus_tag="ACG8H8_001266"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP8986351.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3432..4244)
                     /gene="recO"
                     /locus_tag="ACG8H8_001267"
     CDS             complement(3432..4244)
                     /gene="recO"
                     /locus_tag="ACG8H8_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP8986352.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4387..5286)
                     /gene="era"
                     /locus_tag="ACG8H8_001268"
     CDS             complement(4387..5286)
                     /gene="era"
                     /locus_tag="ACG8H8_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP8986353.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5301..5696)
                     /locus_tag="ACG8H8_001269"
     CDS             complement(5301..5696)
                     /locus_tag="ACG8H8_001269"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8986354.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5680..6156)
                     /gene="ybeY"
                     /locus_tag="ACG8H8_001270"
     CDS             complement(5680..6156)
                     /gene="ybeY"
                     /locus_tag="ACG8H8_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP8986355.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6173..8365)
                     /locus_tag="ACG8H8_001271"
     CDS             complement(6173..8365)
                     /locus_tag="ACG8H8_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP8986356.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8389..9360)
                     /locus_tag="ACG8H8_001272"
     CDS             complement(8389..9360)
                     /locus_tag="ACG8H8_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP8986357.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10066..11445
                     /gene="brnQ"
                     /locus_tag="ACG8H8_001273"
     CDS             10066..11445
                     /gene="brnQ"
                     /locus_tag="ACG8H8_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP8986358.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11601..12047)
                     /locus_tag="ACG8H8_001274"
     CDS             complement(11601..12047)
                     /locus_tag="ACG8H8_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP8986359.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12074..12250)
                     /gene="rpsU"
                     /locus_tag="ACG8H8_001275"
     CDS             complement(12074..12250)
                     /gene="rpsU"
                     /locus_tag="ACG8H8_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP8986360.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12412..12840)
                     /locus_tag="ACG8H8_001276"
     CDS             complement(12412..12840)
                     /locus_tag="ACG8H8_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8986361.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12932..13792
                     /locus_tag="ACG8H8_001277"
     CDS             12932..13792
                     /locus_tag="ACG8H8_001277"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP8986362.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13886..14236)
                     /locus_tag="ACG8H8_001278"
     CDS             complement(13886..14236)
                     /locus_tag="ACG8H8_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP8986363.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14252..15979)
                     /locus_tag="ACG8H8_001279"
     CDS             complement(14252..15979)
                     /locus_tag="ACG8H8_001279"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP8986364.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(16193..17119)
                     /gene="trxB"
                     /locus_tag="ACG8H8_001280"
     CDS             complement(16193..17119)
                     /gene="trxB"
                     /locus_tag="ACG8H8_001280"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP8986365.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17264..18697)
                     /locus_tag="ACG8H8_001281"
     CDS             complement(17264..18697)
                     /locus_tag="ACG8H8_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP8986366.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18697..20100)
                     /locus_tag="ACG8H8_001282"
     CDS             complement(18697..20100)
                     /locus_tag="ACG8H8_001282"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP8986367.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5459 a         4433 c         3637 g         6706 t
ORIGIN      
        1 aacagagaca cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt
       61 gatgatttga tccaagcttg ctaatgacaa agccatggca gctatttgac taagttgtgt
      121 tagtcggttc ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt
      181 ttcgatcagt ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc
      241 aaaacttgct ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa
      301 cagctcagga tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac
      361 acgtgtcaaa gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa
      421 ttgtgctgct gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat
      481 gacatcatga cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc
      541 gatcacttgt tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat
      601 aacatcgcta atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat
      661 gatccgaacg acaccgtatg cttggcgacg taaggcataa ggatcgtttg aaccgcttgg
      721 gatcatccca acagcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac
      781 agaacctact gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg
      841 aatcgctgtc gctacagcag gtttctcgcc tttcagcaga gcatattttt cgcccatgat
      901 cccttgcagt tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc
      961 agcagctcgt tgcagatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat
     1021 acgggcaatg acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa
     1081 agtaacagtt tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata
     1141 aaagaattcc gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatt
     1201 ttccaatttt acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg
     1261 atcattccgt acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc
     1321 aggaacagaa agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa
     1381 attgttgact tcttctagta aatcttcgtc tagatcaata acccaatgat ttttttcggc
     1441 taattcattg gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa
     1501 ttgttcattc aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa
     1561 gaaacgatga cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac
     1621 ttcgtcatct aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc
     1681 tgcccaatgc atcgttacag ggaaagtcag gcttttaatg acatctgcta aacctgtcaa
     1741 tacttctttg gattccttac catgagtgta tttcgttaca tagacatatt ccacaccttt
     1801 taattctcta aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc
     1861 tgctttcgtc cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc
     1921 ttcttcactg tccgcttgtc gttctggaat gtttgttact ttgattgcta aacggcgcgg
     1981 tgtggaaaat gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt
     2041 tttttcttct aattgtttca tgcttggtgt gacaacatga gcaggcattt cttccagccc
     2101 gatttcaagt aataagtctt ttgccatctt attcagcctc cttcacgttt tctggatttg
     2161 ctgtgttttt attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg
     2221 ctacagcacg agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata
     2281 ctgcaccgcg ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg
     2341 ccggatgaac aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt
     2401 tgcttagtag caagtcttgg tcgcttactt caaaagaata tttagaatgt tcatattctg
     2461 gttgtttgaa gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt
     2521 caacttcttg gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg
     2581 gtttacaagg cagtccacca acttgttgga agtacgtgaa ttgtgtgatt tccattccgt
     2641 caagccatac ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt
     2701 caacaaaacg gatgtcgtgt tctagtggat cgattcctaa aaggcgcaag ctttctaaat
     2761 aaagctcttg gatattctca ggtgaaggtt tcatcacgac ttggaattga tgatgctgat
     2821 acaaacggtt tgggttttcg ccataacgtc catcagctgg tcggcgagat ggctctacgt
     2881 aagcagcatt ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac
     2941 ctgctccttt ttctgtgtca tacgcttgca tcagcatgca gccgtttgat gaccaaaatt
     3001 tttgtaatgt taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt
     3061 tcaattttaa agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct
     3121 atgtctgatt acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag
     3181 attcggcagc ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc
     3241 tcccactctc ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta
     3301 ttcattttgc agttttcttg tttctataat agatcctctc agtgaacgat ctatttgatc
     3361 tacggttaat aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta
     3421 tgtttcattt actattcttc ttttttaggt agatcttttt cagacatgtc tttctctgac
     3481 gtctttaata cgtgtgccca agacttcatt tgatcaatga actttttgct tttcaaatgg
     3541 ataccgacat attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct
     3601 tctttcagat ggattccact gattttatca taagaaatag ctgaaaacat ccgaacgaaa
     3661 taaacagctc gaggatctgc atgataacgg tgatcatcca tcgaccagtg tctttcgcat
     3721 atcacaccac catatttgga agaatagtca aattttcctt ttgtttcccc acatacgacg
     3781 cagtgtgtcc agattggtgc aattccgaag cgttgcagta tttggacttc aaaaatattc
     3841 gtgatgattt ctgcatcatc tccttggtcc atccgttgca gtgcttgttg cgtaaactgg
     3901 aacaaatgcg ggtcgtactg atgatcttcg attgccgcat ctactagatt caataaatat
     3961 gtgccatagc cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcgtg gacatctttg
     4021 acactgctta aaaatgacaa accttcactt cgaaaattcc cgatataaac tgcttcagta
     4081 aaagggagca aagccggagc taagggctga ttttgccgat ggatcccttt gatataaaac
     4141 atcattttgc cagaagattc cgtaaagatt ttgactaatt tatctttttc tttgtaatta
     4201 cggctagata aaatcaagcc ttttgattct gcaagctggc tcatattctc cctcctaaaa
     4261 aataaatctg gaaaagcaga ttctgccgat ccagatcatt tatgttgcga tcttccgcag
     4321 cttttctcaa cacgcggatt tttatccagt ttatttctat acctaactta caagaagctc
     4381 agaatatcaa tattcgtctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg
     4441 ccagtctttt tgaactttga cccaaagttc tagaaagact ttgtctccta aaagattttc
     4501 gatatctttt ctcgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat
     4561 gatccctttt tggctatctc tttcgacaat aatcgtagcc tggatatgga ttttatcatt
     4621 ctcgtttcgc ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt
     4681 taaaaacaag actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt
     4741 cacttgatca tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat
     4801 caaacgttcg acgttgtttc cttctgtagc agaaattgga atcacttgtg caaacggcat
     4861 ctgcgttgaa taatcttcga tgattcccaa caactcgtct ggatggattt tatcgatttt
     4921 attgataatc agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc
     4981 tccccgaccg cgtttttgat cagcgctgat cataaataag accgcgtcca cttctttcaa
     5041 cgcactgtaa gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc
     5101 tggtgtgtca ataaaaatca actgagcttc tggtgtagta tatactcctt ggattttatt
     5161 tctagttgtc tgcgccttat cactcatgat ggcaattttt tgacccacga ttcgattcaa
     5221 taatgtggat tttcctacat tcgggcggcc gacgattgcg acaaaccctg atttatgttc
     5281 tggcatatac gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa
     5341 atcaacccaa cgatgacgga aaaacaagcg gtcactaaaa ctgctccagc tgccatatct
     5401 ttgatttttt tcccaatcgg atgaaaatga aaatccgtaa acatgtccac gacattttca
     5461 aaaacagtat tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac
     5521 cattctatcc gatcaagctg gaaaatcacg cccataaaca aagcgaggat tccgaacgca
     5581 acatgtttcc tcatgttgcg ttcttcctca aaaacggttt tgactccttg gacggcaaat
     5641 tccacggaca tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc
     5701 atctaagatc tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc
     5761 atacccgttg atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga
     5821 atggccgtaa gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag
     5881 tacacgaggc aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccctc
     5941 ttcttccaag gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt
     6001 gattacttgg atcgctgcat tgtccatgaa agtaacagac atttctgttt cttctggcaa
     6061 ctctaaatat gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt tctcttctga
     6121 gacttggttt gtttcatcca tgaaagtaat atccattata catcgcttct ttctattctt
     6181 cttgttcttc ttttatcttc tcaacttccg atttcatttt aggatatttg atccgagaat
     6241 ggaaggtact gcttaaaccg ctgatcaatg atttttctac tttacggatt tcttttaacg
     6301 tcaatccact gtcgtccaat tgtccatcgg aaatacgctc ttcaataaga ttatggacaa
     6361 aactttcgat tttttcgctc gttggatggt ccattgctcg tactgccgct tcacaactgt
     6421 cagctatatt gacgattcct gcttcacggc tttgaggttt tggtccagga taacggaatt
     6481 gctcttccgt gacttccggg ttgcgttcct ttgccttaac gtagaaataa cgcatcagcg
     6541 tggttccatg atgctgtctg caaatatcga tcaccatttg cggcatatta taatcttcca
     6601 atattttggc accatctgtc acatgtccaa agataatatg tttactgtct tctggcaata
     6661 agaaattgtg cggattttcc gctcctgaag gcaagttctc aacgaaaaag ctggcgtgtt
     6721 tgatcttgcc gatatcatga taatagcaag ctactcgagt tagtaaagaa cgtccaccga
     6781 tttctgctac agcattagca cttagattgg ctaccatcat gctatggtga tacgtgcctg
     6841 gtgcttcttc cagcaactgt ttcaataaag gatgattagg attgctgagt tcatttaata
     6901 cgatcacact atcatcattg accatcaatt caatataggg gtgcagacct atcgtcagca
     6961 agaatgagaa cagtgcacct gtgaatccgc atactagcgt taaccaagtc gttgcatccc
     7021 caaagctcat tccttggtag ataatcaaaa tgacatccat catcacagga aaaataatga
     7081 cccacatagc agcggataac ccttgttctg agatccgttt gcgtctgacg atcgttgcca
     7141 tgagaccaga aaatacatag gacatcaaaa taactgtcag aaagttcgtg ccaatcgaat
     7201 cataaaaaat gaaaagtgcg gatactgctt gaaaaatacc agccatgatc cctgctcttc
     7261 gattcaagaa aaagcttaaa acaagtggag caaaagccgc ggggtaaaac agcggaatat
     7321 aagcagcttg ttctgtttgg aagagttgga aaaatttcat tagtacgacg cttactgaca
     7381 ttgccgtcac gtagaaaagt acgtattttg ttctttctcc tgcattttgg aattgaagtg
     7441 aattataaat cagtacaccg atttgaagca aaacagctaa aaccatggca atgagtggga
     7501 aaatagaagt cgtttgattc gtcaatccta gtaattccaa ttttttcatc gctgaagcat
     7561 caatttgatt tccttcgcga acaataattt ctccttggaa gatcatcact ggttgtacag
     7621 cttctcgtgc agattgtttt aattcttctg tctttttctc attcaataca tcattaacta
     7681 caatgccttg atccactaag tagcgaatca tttgttgctg ctctgttgtg acattcaaat
     7741 attgaatctg atcttctgct tcttgtttaa aagcttccaa ttcgctttca cgaacttgtt
     7801 tactcatctg ttcgtcaatg agcttcaaac tttcatccct aaccgtgtga atttccgttt
     7861 cagtcatttc aaatatcgtc gaataaaaat tgttcggcag tttttggtaa aacgcaacgt
     7921 tttctgcatt cacgttttca aaatttttct tcagtgtagc aatccgctct tctgtagtag
     7981 gtttcgggat attttcatct tccttcgcct tatctacctt gtcattataa gatttgttga
     8041 tggtatcatt cgtcttatcg atcaattcga ataattgctt gatccgattg ttctggtcat
     8101 ctgccagatc tttttgataa gtatattctg gagtcactgc ttctgctgcc agttttcttc
     8161 tttgttcggt ttcttccgtg ttctcaatcg ttttattcgc acggatactc tcttctgcta
     8221 cttggccttc tctgaaatca ttggcctttt gtttgacact actaaatgta acgccgcaga
     8281 caatgataaa aaacaagaac agaaggaaag gcaatgctgc ctttccgagt ttttcttgta
     8341 gtttctgcag tgttttattc aacatgtgct tatcctccta agcaaagctt attcttgatg
     8401 aagatccgcc ttttcataag ctttgatgat ttcggcaact acagggtgcc gaacaacgtc
     8461 acttgcttca aaattaacaa aatcgatttt tttgatttgc tttaatgttc gttctgcatg
     8521 aacaagacca cttgtcgtcc cacgaggcaa gtctatctga cttgtatccc cattgacaat
     8581 catttttgat tgataaccga gacgggtcaa gaacattttc atctgagcga ctgtcgtgtt
     8641 ttgtgcttcg tccaagataa cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa
     8701 aggtgcgatt tcgatcacgc ctcgttccat caatcggttc gtatgatcca atccaaaaat
     8761 ttggtataaa gcatcataaa caggccgcag ataaggatcc actttttctt tcagatcccc
     8821 gggtagaaag cctaggtttt cccctgcctc cacagcgggc cgcgtcaaaa tgattttttg
     8881 cacttcgcct ttttttaggg cagcaattgc taatactacg gcaagaaacg ttttccctgt
     8941 tccggctggc cctacaccaa atacgatgtc atgtttggct accgattcaa cgtatttctt
     9001 ttgaccgctg ttttttaccc gtataggttt tccattacgg tctttgacga tctcttcctc
     9061 atacatctca acaaaataat ctaatgtacc tttttgcccc atcttcaatg ccgtcacaac
     9121 atctggcgta cttacgtgga ttcctcgttt gatcaattcc tgtaatgtcc gaatgactga
     9181 ggcagccaag gaaatctggg attgctcacc aatgatctga atgatttctc cacgtgtatg
     9241 gatggttgtt tccgttgttt cctcgatcag ttttatatgc ttgtcgtgtg cacctaaaag
     9301 catgctgaca tcatcagcag ctgtcaatct aatctctaaa gaactagact cttccgtcaa
     9361 aaatagacat cccctttttt tcgattcatt ctttcctatt ttaccatact cagaagaaac
     9421 tatatagcaa ataagaaaag gttgtaaaag agtcgtttaa ccatacgtac caagaaacgt
     9481 taaaaaatag ccgtttccat tcgctaaagg tctcgttttg ataaaaagaa gctgagaatg
     9541 taagttatat gaaagtggag aacaaaatat tccgtttcat tcagcaggta ttctctaggt
     9601 atttttcttg tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa
     9661 aacaggtgta ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag
     9721 gctattcaac gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata
     9781 gacgaacatc atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat
     9841 caggctagcg gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa
     9901 aaaaggtggt accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt
     9961 atattttaag gtggtggttt tcatggaatc tgatattagt ggcattacta attgctgcaa
    10021 cgtttatatc gtcaatcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa
    10081 acttattcat ggaaacaact attgatggtc agttcactta tcttcgggat gttcttcggg
    10141 tcaggtaatc taatctttcc tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt
    10201 gctggcgtag gatttgccat ctcgggcgct cttttcccat tactagctat cttagctgta
    10261 gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct
    10321 gccttgttct taatccttgt tcatttgacg atcggaccat ttttcggtac accacggaca
    10381 gctgctactg cttttgaaat ggctgctaaa ccatttttgc ctgaaaaata taattcatta
    10441 ggtatgtttt tattctctgc tcttttcttt ttacttgctt atttccttac tgtccatccc
    10501 acacggttgg tcaaatacgt cggaaagtat ctaaactcct tgtttcttgc attgttagca
    10561 atcatttttg tcgtttcttt attccaccca atgggaaatc taaatcaatc tgctgcagat
    10621 acttaccaaa caaacgcttt aacaaatggt gtcctagaag gttataacac ggttgatgca
    10681 gtagccttgc ttgccttaag tgtcactttt gtccatgcag tccgtgacct tggtttcaaa
    10741 gataaaaaag tcacagaatt aacagcaaaa gctggtagcc tgagcgttgc gctagaagtc
    10801 atgatctatt ttggtcttgt tcttctcggt gcgatgagtt tgaataagat gtctttatca
    10861 gaaaatggcg gaactgcact ttctcagatc actaattttt atttaggtaa cttcggcgct
    10921 agcttcttag gagtgatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt
    10981 tcttttgcgc aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg
    11041 ttaacgactt tcgtctcttt tgtcgttgca aatgctggtt tagacaatat catccaatgg
    11101 tctttaccag tattgatgct tttgtatcca ttgtcgcttg cgttgatctt gctttcctta
    11161 acagcaaaat ttttccaaaa gacaccattt gtctatcaag taacgatgtt gtttgctgct
    11221 gttcctgccg tattggatat gctggcaaat tcgcctgctt tagtgagtca acaaagagtt
    11281 gtagcaagca tgctcgaatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc
    11341 tggatggtcc caacgttact tggttacgct ggcagcctac tgttttatta cgcctatcgc
    11401 ctatcagggt ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa
    11461 tttaacataa gaaatattaa acaggaaaaa accagctaga tgaaagaatc ctattctttc
    11521 gtctagctat ttttttgttc tcttttttac gcgcacaaaa aaactgcgga caatgattgt
    11581 ccgcagttta aatgataaaa ttaagattgt aatagttctt tcacaatggc atttacttgg
    11641 tttccatctg cttttccttt gactttaggc atgatcgcac ccatgacttt gccaaattct
    11701 ttcattgatg aagcaccagt ttgtgtgatt gcttcttgaa caagttgtcg aatttcttcg
    11761 tcggaaagtt gttccggcat gtatttttcg actatcacaa tttcactttt tactttttca
    11821 gctaaatcgt cacgtcctgc ttcttcaaac tcatgcaaag aatcacgtcg ctgtttcatt
    11881 tcgcgagaca acaccgttaa ttcttcttct tctgtcaagt cgtgaccttc tttaatctgc
    11941 tcgttttgta tagatgactt gatcattcgg atgacttgta aagattcctt atctttcgct
    12001 ttcatcgctg ttttcatgtc atcgtttaat ctacttagta gtgacacact accaactcca
    12061 attcatcaca gaactagaat tttttgcgtt ttctagctgc ttcagatttt ttcttgcgtc
    12121 ttacacttgg tttttcgtag aattcacgtt tacgagattc ttgtaaagta cccgcttttg
    12181 acacggaacg tttgaagcga cgaagagcat cgtcaagtga ttcgtttttg cgaacgactg
    12241 tttttgacat gttaattccc tccctccgag cttaaaaagt aaccgtaatt gttattgaaa
    12301 tatatctcaa aaaacaagac tgtcctgtaa atattatatc taatgctgtt ttgaccgtca
    12361 aggaaatctt gctttttttt aaaattatca aatgttattt tatttaaatc cttattgaca
    12421 attttcacat cttccgaaaa tctcgaatct atgcccttcg atcgtacatc caccgagctg
    12481 ttcttcgaaa taattcattg gacacatatg gatttctttc gttgttccgc acaccgtaca
    12541 gataaagtga tggtgatggc cgatttcccc ttgacagcaa tgaaaacgaa acttcatctc
    12601 tccatttaaa tcggtttctt ccagtaaatc gatttctgca aaatcatgga gatttcgata
    12661 aatcgtatcg taacttactc ctttgaattc ctgattcata tagtcaaaaa cttctttagc
    12721 ggatacgtag cgattgcgct tcactaaata agttaacaat gcttctcttt ttttagtata
    12781 tttaaaaccg ttatttttca atgtttctag tgattgttca atcaatgtct gttttttcat
    12841 gacttattcc tccaaatcaa aatgattacg aagtacacat tctagagtat ggtataataa
    12901 acataaaaaa aatgcaagtg aggttttatc catgtcttcc gattctacag aaatcgttac
    12961 tttttttgtc atctctgact ctgctggaga aactgcgaca aaattagcgc aagccactat
    13021 ggcccaatat cctaccgttg agttcaattt attccgcaga acatttgtga cagatgagaa
    13081 aacattgatg caggcactgc aagatgcact aaaagaaaaa gcccttgtcc tgcacacatt
    13141 gatcaatcaa gaattgatcg agatgacgcg ttctttttgt gaagaacatc aattatttag
    13201 ttttgatgtg atgactccgc cgatatcaga aatcgagcgc ttaactggta tcaagcccat
    13261 tcggcaacct ggcgcccttc atttattgaa tgagaactat ttcaaacgga tcaaagcaat
    13321 ggaatttgct gtaaaatacg atgacgggaa agatcctcgt gggtttctag aagcagatgt
    13381 cgttttgcta ggcgtatcca gaacttccaa aacgcctttg agcttgtttt tagctaacaa
    13441 aaatttaaag gttgccaatc tacctttgat cccacaagca catattccca aacagctttg
    13501 ggagatcgat cctaaaaaaa tcgtgggact gacgaataac ccagatattt taaacaatat
    13561 ccgtaaagaa cggatgcgct cttacggatt aaatcctgat actgcttatt ccgatatcga
    13621 aaaaatacga gctgaactcg attttgcgaa tgatctttat gaaaaattgg gctgcgtagt
    13681 aatcaatgtc gcttctttat ctatcgaaga aacagcttca cttattttga atgctttaga
    13741 cctagaagat catagttatt atggaactga aaccaccgaa gaaacagact aacagttttt
    13801 cttgtctttt tcttaaaaaa actggactgt gtcagcgaag aagattcgtt gatacagtcc
    13861 agtttgtctc tatttgcacc tatcgttatt gatgttccac atgtttgagc gtctggttca
    13921 aaatatccag tacgtgagaa tcatcaagac tatacaaaat cgcttttccc tcacgatggg
    13981 acttgacgac atggttttct cttaataaac gcagttgatg cgatacagca gattgttcca
    14041 tttctactgc ttgggtgatt gctgtcacat tttgttcccc gtctttcaga tacagcagaa
    14101 ttttgagacg agtggggtca ctcaacattt tgaatagttg gctgactttc tctacttttg
    14161 ttttttgcgt gtctggcaat ccattcacct ccaacttgaa aaaagactga cttctaaagt
    14221 ttaccacttt ttccatcagt ctcaagtttt tttattcttc tgtcattttt ttgataatcg
    14281 cttcataagc ggctactttt tcagctgcgt cttctgaaga gtttcctttt acaccgatat
    14341 aaaatttgat tttcggctca gttcctgaag gacggatagc gatccagcca ttttcttcca
    14401 gcgtatactt caatacgtca gatggcggtg tcgttagttt ttctacgttt ccttcacggt
    14461 ctgttctcgt caactctttg aagtcttctg tttggatgac ttttgctccg gcaaattctg
    14521 tcggtgcttc ttcacggaat ttttccatca aagcagagat ttttgcagaa ccttcttgtc
    14581 cgctcattgt cacagaaatc gttttttctg cgtagtagcc atatgtttca aaaatcgttt
    14641 gcaagccgtc atacaacgtt ttgccttgtt ttttatagta agctgctact tctgctaata
    14701 agagcaatgc ttgaatcgcg tctttgtcgc gaacgaaagg tttaactaaa taaccgtaac
    14761 tttcttcgaa tccgaacata aaagtatggg aatgatcttc ttcaaattgt tgaatttttt
    14821 cagcgatgaa tttgaatcct gtcaatacat tgaacatggt agccccatag tgttccgcaa
    14881 ttgctgtagg caattcgcta gaaacgattg atttcaatac cgctgcattt tcaggtaaag
    14941 tgcctcgcga ttggtgtgct tccaaaatat attgagcaaa cagggctccg atttggttcc
    15001 ctgtcaatac ttcataggag ccatttggca aacgaacggc tgcgcctaaa cggtctgcat
    15061 caggatctgt tgcaatcagt aagtcagcgc cttctttctc gccaagtcgc atcgcatact
    15121 cgaaagcaga gtgttcttct ggattcggtg atttgactgt agtgaaatcc gggtctgcga
    15181 cagcttgttc tggaactagt acgaattgtt cgaatccagc ttgtttcaac gcacgttcgc
    15241 ccaacatttt tcctgttccg tgaagtggtg tgtacacaag tttcaaatct ttacccattg
    15301 tttccaccaa ttcatgatca atcgttactg ctttgatttc ttctagataa gcggcatcca
    15361 cttcttctcc gatgatcgtg atcaatccgc tatgttcagc ttcgtcgtca gataatactt
    15421 caacttttaa cggattgctg acttcacgga catatttcgt caatgcatct gcgtctgcag
    15481 gaggcatttg tcccccatct tctccgtaga ctttatatcc gttgtaagct gctggattgt
    15541 gggatgcagt taccatgatt cccgcaaacg tcttcaaatg acggactgca aaagaaagct
    15601 ctggtgttgg acgtaaactt tcgaatacat aagaagggat attgtgatgt gctaatgttt
    15661 tggctgcttc catggcaaat tctggtgatt gatggcgtga gtcataagcg atagcgacac
    15721 ctcggcgttt tgtttcttca ccttgtgtat ccatgaaacg tgcaagtcct tctgttgctt
    15781 gtcttaccgt atagatgttc attcgattga tcccaggtcc taagattccc cgcatgcctg
    15841 cagtaccgaa ttccaacggt gcataaaaag catcttcaag tttttcagga tcttcaccca
    15901 gatcaattaa ttgttttttt aagttttctt ctaaattttc ttcatgtacc cattgttcat
    15961 acacttgttc ccaagacatg aaaataacac ctctttctga aatatggttc ttaataagta
    16021 tagcattcac ttattagata tgaaagtacg aggtctaaaa aacgcttaaa tcaagaaagg
    16081 attcttttat caagcacggt tgcaagtatt cgcataaatc ggattcagta gatttcacgc
    16141 gaaaaaaaag agtaaaagaa caattgaaac cgttcttcca ctctttttaa acttatgcag
    16201 ttgtttcttc taaagattcc aagtatttat acacttgttg tccagcgatc ccaccatcac
    16261 caacagcagt agtgatttgt cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc
    16321 ctggtatatt cgtcttcatt tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc
    16381 ctgcttctct aaatggtgca gttaatggat ccagtccaac atagatgaat actccgtctg
    16441 ctgccaaatc aaactcattg ccggttttga cattacgtgc tttcacacct gtcactacca
    16501 tttcattccc aacgatttcg tcaacgactg tatcccacat aaaggaaatt ttttcattcg
    16561 caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg tggacgatga
    16621 ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag tctcctcctc
    16681 cgatcactac tagacgtttg ttacggaaaa acgcaccatc acaaactgca cagtaagaaa
    16741 cgccacgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg atacagcctg
    16801 ttgcgataat gactgttttt ccttcgtaag ttttatcttc acaaataact tctttgtggc
    16861 tgccgtgatc cttgatatct tgcacgattc cataagcatt ttctgtccca aatcttgaaa
    16921 caccttcata catcttataa gctagatcag gacccatgat tgaatcgaaa ccaggatagt
    16981 tttcgatttc tgccgtattg ttcatttggc cgccaggggc gccttgttcg atcatcaata
    17041 cggataagtt tgaacgtgaa gcatataaag cagcagtcat cccagcagga cctgcgccaa
    17101 taacgattac atcatacata tgtagattct cctttactta atcaagtaag catactttac
    17161 agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct ttattatact
    17221 cgaaaaatcc gctgaaaaac aatagacttt tacaaatcat tgatcaatca ttcgaaagat
    17281 tttctccatt tgaatcaatt acttttttat accagttgaa tgatttcttc ttcgaacggg
    17341 ctaatgtgcc atttccttcg ttgtcacgat cgacataaat gaatccgtac cttttcttca
    17401 tctcacctgt accagctgat actaaatcaa tacatcccca agtcgtatag cctaatagat
    17461 cgactccatc taattctact gtatctttca ttgcctggat atgtgctgcc agatagtcga
    17521 ttcgatagtc atcttccaca taaccattct catcgggcgt atcgactgca cctaagccgt
    17581 tttctacaat aaatagtggt ttttgataac gatcataaat atcattcatc gttattctca
    17641 agcctaatgg atcaatttgc caaccccatt cacttgcttc cagatatgga tttttgacag
    17701 aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcagtagtc gaaactcgag
    17761 aagaataata agaaaaggag ataaaatcaa cagtatgttc tttcagcaat gcggcatccc
    17821 caggttccat tttgatctga atgccatttc gttccatttc tttcaatgca taagcgggat
    17881 attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgat tttctagaag
    17941 cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc agcatacagc
    18001 ctactttgtt ctccggatct acttcatgag caatcttcgt tgcgatggca cttgcaacca
    18061 actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct tcaaaataca
    18121 gccctgcgcc catgaatggt gcatgaagga tcatattgat ctcattgaat gtcaaccaat
    18181 atttgacaag tcctttatag cgattgaaga tgactcggca cagattttca taaaaaccaa
    18241 cgagttctct agaacgccac gcaccatatt ctttgatcaa atgcatcgga caatcaaaat
    18301 gtgtaatcgt cacaagcggc tcgatcccgt acttttgaca ttctttaaag acatcctcat
    18361 aaaatttcaa accagattca tttggctcat tttcatcgcc cattggaaaa atacgactcc
    18421 aagcaatgga catccgataa gtcttgaacc ccatttctgc aaacaaagca atatcttcct
    18481 tataatgatg atacatatcg atcgcaattt ttgctggata aaaatgatct ttatcaaatt
    18541 caaacatttt cttttttcca gtaatgacag gaaaacggtc ttctccgatt ggcacaacat
    18601 ctacgttcgc taacccacgt ccgccttcat cataaccacc ctcgcattga ttggctgctg
    18661 tagcaccgcc ccataaaaaa tcttttctga atcccatcat acttcctcca ttacatgtgt
    18721 attgcttgac aattctcttg ccagttcatc ttggacttcg attccttctg cttttaaccg
    18781 gctgattgtt tccttgaatt gcggtaaata ctgctgatgt gcaataaaca actcatccat
    18841 gatccgtttt gctgtctttc ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc
    18901 tgttccataa tcaccagtca cggctgcttc gatcgtcagc aattccatat tcttcattac
    18961 ttgcaaccag cctctttcag cagcaggaag ggaaccaaac gctacatttc gtgcgccttg
    19021 tgcgccaaca taggcagtga cttctactac acaatctgcc ggcagatcag gaacagctcc
    19081 atcattcttt gtagacacca cgatctgtgt ttctttattt ccatagatcg aagcaatcgt
    19141 ttcacaggca gcatctgaat aataagcacc acctcgtttt tgcaattgtt caggtttatg
    19201 atccaattct ggatctttgt ataattcaaa cagctctgct tctgtttctt tcacttgttg
    19261 cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccaacattt cttcttcacg
    19321 gtagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt gttctttgaa
    19381 aaacgggatg tcaaagatat ttttcggcaa tccattgtct tcatcgtata aacgatcaat
    19441 caaatctgct gtgcggtcat ttcctttatt atctgctact ttgtgccaat ggaagtgatt
    19501 tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg ctggttctgt
    19561 catcgtcgcc attactggca cattgcataa tccgatcact ttatcccatt tgccatatct
    19621 gataactgct tctgtcacca tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg
    19681 acataaacgc ttcatgtctt caacgattgc caaaaccact ggaacggtac gaaaagcttt
    19741 gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac tagggatccg
    19801 ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc
    19861 atctttcaat gcttcttccc gatccaaagt tgcatagatc ttgacttcat acggagaagc
    19921 atcccacata cgctgagaca tctgactgac gatctcaagc ttttcttttc cttcttctac
    19981 atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa atccttccat
    20041 caactcgggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc cttttgccat
    20101 gttctcactc ctttgctttt tacactttta ttatcttttt tgtaaacaaa acaaacaata
    20161 gaaattcgtt tctttccagt aacatttaca aaagttagtg ttttctactg gatttttgta
    20221 aatatcttgt caaaa
//
LOCUS       DAZKAO010000043        20185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000043 DAZKAO010000000
VERSION     DAZKAO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.043"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1017
                     /gene="proB"
                     /locus_tag="ACG8H8_001283"
     CDS             211..1017
                     /gene="proB"
                     /locus_tag="ACG8H8_001283"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP8986368.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1014..2261
                     /locus_tag="ACG8H8_001284"
     CDS             1014..2261
                     /locus_tag="ACG8H8_001284"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP8986369.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2337..2681
                     /locus_tag="ACG8H8_001285"
     CDS             2337..2681
                     /locus_tag="ACG8H8_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986370.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2798..3124)
                     /locus_tag="ACG8H8_001286"
     CDS             complement(2798..3124)
                     /locus_tag="ACG8H8_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986371.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3497..4699)
                     /locus_tag="ACG8H8_001287"
     CDS             complement(3497..4699)
                     /locus_tag="ACG8H8_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8986372.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4816..5331
                     /locus_tag="ACG8H8_001288"
     CDS             4816..5331
                     /locus_tag="ACG8H8_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8986373.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5530..5976)
                     /locus_tag="ACG8H8_001289"
     CDS             complement(5530..5976)
                     /locus_tag="ACG8H8_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP8986374.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6023..7501)
                     /gene="msr(C)"
                     /locus_tag="ACG8H8_001290"
     CDS             complement(6023..7501)
                     /gene="msr(C)"
                     /locus_tag="ACG8H8_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP8986375.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7855..8484)
                     /locus_tag="ACG8H8_001291"
     CDS             complement(7855..8484)
                     /locus_tag="ACG8H8_001291"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP8986376.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8468..8932)
                     /locus_tag="ACG8H8_001292"
     CDS             complement(8468..8932)
                     /locus_tag="ACG8H8_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8986377.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9419..10687)
                     /locus_tag="ACG8H8_001293"
     CDS             complement(9419..10687)
                     /locus_tag="ACG8H8_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP8986378.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10918..11655)
                     /locus_tag="ACG8H8_001294"
     CDS             complement(10918..11655)
                     /locus_tag="ACG8H8_001294"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8986379.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11722..12291)
                     /locus_tag="ACG8H8_001295"
     CDS             complement(11722..12291)
                     /locus_tag="ACG8H8_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP8986380.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12419..13291
                     /locus_tag="ACG8H8_001296"
     CDS             12419..13291
                     /locus_tag="ACG8H8_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8986381.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13633..14505
                     /locus_tag="ACG8H8_001297"
     CDS             13633..14505
                     /locus_tag="ACG8H8_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8986382.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14502..16460
                     /gene="nagE"
                     /locus_tag="ACG8H8_001298"
     CDS             14502..16460
                     /gene="nagE"
                     /locus_tag="ACG8H8_001298"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP8986383.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16711..17562)
                     /locus_tag="ACG8H8_001299"
     CDS             complement(16711..17562)
                     /locus_tag="ACG8H8_001299"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8986384.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17685..18152
                     /locus_tag="ACG8H8_001300"
     CDS             17685..18152
                     /locus_tag="ACG8H8_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP8986385.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18415..18897)
                     /locus_tag="ACG8H8_001301"
     CDS             complement(18415..18897)
                     /locus_tag="ACG8H8_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8986386.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18997..19860)
                     /locus_tag="ACG8H8_001302"
     CDS             complement(18997..19860)
                     /locus_tag="ACG8H8_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8986387.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6069 a         4157 c         3663 g         6296 t
ORIGIN      
        1 aactactcct attctaacat atccgaggct gtaaaagact ttctttacaa attcttcaag
       61 aaattctgct atcttaaagt aaaccattaa ttcgttcttt ttccaaacga ctacaagttg
      121 tacaaaaaaa ctatttttta catgacttta ctcgtcaaaa caagtagaat agaacgaaat
      181 taatgagtgg atatttgatt gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga
      241 attattgtca aagtaggaac aagtacattg atttatccca atggaaatat caatcttgcg
      301 gcaatcgatc agttagcttt tgtcctttct gatcttagaa atcggggaaa ggaagtgatc
      361 ttagtctcat caggagctgt aggcgttggt atgcataaat tacatttgtc agaacgtccg
      421 actttgattc ctgaacagca agctgcggcg gctgttggtc aagccgaact gatgaatatc
      481 tataatcaaa gattcagtgt atataatcaa cagacggcac agatcctcgt gacaagagac
      541 gtggtagatt atcctgaaag tcgtaaaaat gtgaggaata cattggaaca gcttttgatt
      601 atggacacga tccctatcgt gaacgaaaac gatactgtag cagtggatga attagatcat
      661 ctgacaaaat ttggcgataa tgatcagctg tcagccatca tggcaaaact tgtcgcggca
      721 gaccttttga tcgtcctttc tgatatcgac gggttttatt cagataatcc gactacaaat
      781 cccgaggctc agatgtatcg ctatatcgat aaaatcgatg aaaagattct ggcacaagca
      841 ggtggtgctg gtagcacatt tggaacaggc ggtatgcaga gtaaattaaa agccgcttct
      901 aggatatttg aagcaaacag cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt
      961 tttgatattc ttgcaggaaa agaagtaggt actttattta aggaggactt gaaatgactg
     1021 acttgatgca attaggacaa caggcaaaag atagcgcgca acaactagca cttatgccta
     1081 caaaccgcaa aaatgagcta cttgttcaaa tggcacaaac catcaaaaag aatcaacaag
     1141 cgatcattga agcaaatcat aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa
     1201 cgatgcaaga ccgcttgcag ctgacacctg agcgaatcaa tgccatggct acagagatcg
     1261 aaaaaattgc ttcgttagac gatcctattg gaaaagtaga tgagatgtgg acgaacaccg
     1321 acggattgag aatcggcaaa aagcgcgtgc cactaggcgt gaccggcatc atttacgaat
     1381 ctcgtccaaa tgtcacgaca gatgcagcta gtctagcatt caaatcaggg aatgccgtta
     1441 tccttcgcgg tggcaaagaa gcattcttct cgaatcaatt attagtccaa ttattgcagc
     1501 aagtactggt cagtgctggc gaatctcctt atgccatcca gtttgtcgat gatacttctc
     1561 atgaaactgc aggacagttg atgcgattga cagaatactt agatgtgctg attcctcgag
     1621 gcggagcaaa attaatccaa cgagtcaaag aacaggcaac ggtacctatc attgaaacag
     1681 gtacaggaaa taatcacgta tatatcgata aagaagcaca gttatctatg gcagtgaaca
     1741 tcatcgtcaa tgcaaaagct tcccgtccat ctgtctgtaa cgcggcagaa acattgctga
     1801 tccattctga gatcgcacct tttttcttgc cagctattga aaaagaactg gtagaacacg
     1861 gggtctcttt acgtgcggat gctcgtgcgt tagaatatct agagacggct gctttagcag
     1921 aagacacaga ttgggatact gaatatctag attatatcct tgctgtcaaa gtggtcgatt
     1981 ctttggatga agcaattgca catatcaatc ggcataatac taaacattca gaaacgatcg
     2041 tgacagatag ttatgcggct agtcaacgtt tcttaaatga agtggacgcg gcggctgtct
     2101 atgtcaatgc ctccactcgt tttacagacg gatcggtatt tggttttggt gcagagattg
     2161 gtatctctac tcaaaagttg catgcccgtg gtccaatggg gttaaatgaa ttaacttcca
     2221 caaaatatat catttatggc gatggacaaa tccgcgaata atgtttcgtg tgaaacatta
     2281 ttcccgcctt ttgccttttt tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt
     2341 ttgaaatttt aatgattttt tttatttacc ttatttctct gaatatcgct gcttttctgg
     2401 gagtcggtat tttatccctt ttctttcaat tcaaaaaacg aagcataggg agtcaacggg
     2461 aaaaatggtc gcagtatttt gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca
     2521 ttagttacat ggttgcatta tgcttattag cgacaatcaa ttactattct tttttcgatc
     2581 attcaatcgc ttatacgatt acgctactga ttgctgggat ttttcactta tcttataaat
     2641 atcagctgaa taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc gtacgataag
     2701 tagagtgcct tgaagatcgg caaaaaactg ccggactttt tagaggctgt gacaaagatt
     2761 ttgtcacagc ctctttttcc atataaacgg cgttcgccta ccagctgcct ggtaaatctg
     2821 tttcctcttc gttaagaagc gaaaatccgt caaatgtttc gccatcataa taacgaagca
     2881 gctcatccat ggacaagttc atcgtttttt ctttttcttc ttccgctgat tcagccagta
     2941 gctgttcccg atacacctga tacataccag ctattttttg ctgcatcgct tttctagttt
     3001 caccttccgc attgattatt ggcaattctt tgatatagcc acgataaatc aaccgatcat
     3061 ctgaaaattc aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca
     3121 gcatctatta tccccccatt ctttttctta aatgaatttt agcttaccta ctgaaaattt
     3181 tccagtttca ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt
     3241 tttccgaata aatggtgttc ttctgccaac tcctcaatca taagtcacaa tattcaacaa
     3301 acatgagaaa ctgttttttg aaattgttcc ttgtgattca gagttaaacg acagtctcac
     3361 aacctcttgg taaacggcgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
     3421 aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
     3481 cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga
     3541 tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa
     3601 tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa
     3661 aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg
     3721 tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa
     3781 ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg
     3841 ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg
     3901 aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat
     3961 agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca
     4021 atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa
     4081 ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac
     4141 cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga
     4201 ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga
     4261 ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa
     4321 ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag
     4381 ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt
     4441 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
     4501 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
     4561 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
     4621 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
     4681 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
     4741 gtaaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
     4801 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
     4861 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
     4921 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
     4981 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
     5041 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
     5101 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
     5161 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
     5221 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
     5281 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
     5341 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
     5401 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
     5461 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
     5521 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
     5581 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
     5641 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
     5701 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
     5761 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
     5821 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
     5881 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
     5941 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
     6001 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
     6061 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
     6121 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
     6181 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
     6241 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
     6301 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
     6361 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
     6421 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
     6481 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
     6541 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
     6601 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
     6661 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
     6721 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
     6781 ccggtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
     6841 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
     6901 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
     6961 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
     7021 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
     7081 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
     7141 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
     7201 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
     7261 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
     7321 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
     7381 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
     7441 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
     7501 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
     7561 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
     7621 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
     7681 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
     7741 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
     7801 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
     7861 gaacgtagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
     7921 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
     7981 caactgatct gcttcgactc caccaattgg atttgtttca tagccatgat cacgcgcaac
     8041 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
     8101 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
     8161 tggcatcttc cctgattcgt aagcttggtt atagatttct tcgccatatt cgtaacattg
     8221 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
     8281 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
     8341 ttgcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
     8401 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
     8461 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
     8521 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
     8581 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc tcgattttct
     8641 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
     8701 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
     8761 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
     8821 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
     8881 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
     8941 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
     9001 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
     9061 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
     9121 ttattgattc ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc
     9181 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
     9241 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
     9301 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
     9361 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
     9421 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
     9481 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
     9541 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
     9601 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
     9661 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
     9721 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
     9781 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
     9841 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
     9901 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
     9961 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    10021 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    10081 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    10141 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    10201 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    10261 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    10321 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    10381 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    10441 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    10501 aaattgtatt ttctgagaca gtagctgact gcaaagtact ctctgtatcg ttttcaattc
    10561 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    10621 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    10681 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    10741 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttctttttg ataaatttgc
    10801 tttttctaca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    10861 tggtcgtttg aataactatt taacaagtaa ttctctttgg atattatccg ttatggatta
    10921 tacttcctgt gtcgtcggac ggataatcat gttactgaca gacatccggt ctggcgtttc
    10981 tatcgcaaat gctacagcag aagctatatc ttcggaggtc aatccccatt ctttttgcgc
    11041 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    11101 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    11161 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
    11221 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    11281 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    11341 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    11401 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    11461 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    11521 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    11581 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    11641 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    11701 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    11761 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    11821 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    11881 atttttcttg atgtggattg gctttactaa caagcctctt cgttcttttg gatcggttag
    11941 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    12001 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    12061 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    12121 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    12181 cgctgctaaa ggctcttcgt tttcagcttg gatctgatgg atcacttcaa agagcggatc
    12241 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    12301 tccttttatc cttactttat aacggacggc ttatcatgtc taatacttat aaaacatact
    12361 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    12421 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
    12481 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
    12541 agaacttggt gtgacacttt tcactcgggg ctcccgagag atacagttga cggattctgg
    12601 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
    12661 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
    12721 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
    12781 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
    12841 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
    12901 ccaagatcgt tggggacttt tgatacatcg aaatcatcct ttagcaaagc aaaccgtcat
    12961 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
    13021 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
    13081 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
    13141 tgggatcatc aatacttatg ggacggaaaa aatattcatc ccactcgatc cgccattaca
    13201 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
    13261 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
    13321 tttaacaacg aataagataa gcaaaagatc caacgattaa ataacataag tttcttaatt
    13381 tatttcttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
    13441 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
    13501 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
    13561 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
    13621 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
    13681 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
    13741 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
    13801 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
    13861 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
    13921 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
    13981 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
    14041 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
    14101 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
    14161 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
    14221 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
    14281 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
    14341 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
    14401 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
    14461 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
    14521 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
    14581 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
    14641 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
    14701 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
    14761 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
    14821 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
    14881 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
    14941 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
    15001 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
    15061 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
    15121 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
    15181 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
    15241 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
    15301 aaaacaaagc aaaaacagct tccttgatgc tagcagcagc ttttgcttca ttctttactg
    15361 gtgtgacaga accacttgaa ttttcattta tgttcgttgc ttggccatta tatgtcttgc
    15421 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
    15481 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
    15541 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
    15601 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
    15661 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
    15721 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
    15781 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
    15841 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
    15901 tcgttgcaga tgagatggca aaactacatg gtggtgctgc agcacgacct gcacaaacaa
    15961 acaccgtggt taaaacagaa acatttgccg gtgaaacgat cgaacaagat atttacgcta
    16021 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
    16081 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
    16141 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
    16201 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
    16261 tatatgtaga agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
    16321 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
    16381 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
    16441 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
    16501 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
    16561 ccaactccgc ggtaataagt ccagctaaac gaaacatatg aagagctatt ttttatttga
    16621 ctcttcttat tacttgatgc agaacagctt tttcacaaaa tttttcttca tactcaaaac
    16681 taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt cttttaatgc
    16741 tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag aggaaatagc
    16801 taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg cttcttgagg
    16861 gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa taatttctaa
    16921 ctctttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag ttagattgcc
    16981 ggataattct aagatttctg gagagtcact ttctactagt acttttgctg cagcttctgc
    17041 atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa cgaattgacc
    17101 agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat tattccgtaa
    17161 aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt gatctggcgc
    17221 taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat aagatacccc
    17281 agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc ctggaacgcc
    17341 agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt ctaaatcatc
    17401 ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt tttcttcact
    17461 acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt ccagcgcata
    17521 accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca ctccctattc
    17581 tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat cataaagagt
    17641 gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag tattctctgc
    17701 aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat acccgatcac
    17761 tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca
    17821 tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg cagccttccc
    17881 ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc gaaggtaata
    17941 cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc
    18001 aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta
    18061 agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa aaaagaccag
    18121 aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg catttttctg
    18181 atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt gtcatgcccc
    18241 tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt
    18301 tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa gctatttccc
    18361 atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat attatcatgg
    18421 tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa ctcccatttc
    18481 gactacagca tttggacccc caacataggc gtaatcttct acttctctta aaacatgccc
    18541 aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta aagcgcttac
    18601 aacaagatac gttccttttg ggaatgggat gattcgcgta gtgtttggaa catcctgact
    18661 cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga ctgtaaagaa
    18721 ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga taactttttg
    18781 ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac tcttcaattc
    18841 cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact ttgccatttc
    18901 tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa atgacacgat
    18961 tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt tcactgcaat
    19021 ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag agatctcggg
    19081 agcttcgact aacccataat ctgaagaagg aagccattct gcatagattt tcccccaagt
    19141 atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag tgtgttcttc
    19201 tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa agccaatcat
    19261 gtgcatcaaa ctgccctttt cttccaagcg gtctccatca aatccataag aaacgttgat
    19321 tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag tctccgtgat
    19381 cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag gaactttttt
    19441 cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca tcgattttcc
    19501 tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct
    19561 gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt
    19621 ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagaa gatcgcgatt
    19681 agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg caataaacgt
    19741 aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta ttttatagga
    19801 aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat ctttaagcat
    19861 atttatcttc ctccttgatt gcctttttat catattatca aagaaagtca attacttgat
    19921 gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa gaaaattctc
    19981 aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt acctttagaa
    20041 aaataaattt tggggaagac agattctaag taaacaaagc ggtaaaaata tcttttattc
    20101 ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa actgctgatc
    20161 agaacgctgc ttggattttt ctttg
//
LOCUS       DAZKAO010000044        20170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000044 DAZKAO010000000
VERSION     DAZKAO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.044"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..512
                     /gene="mraZ"
                     /locus_tag="ACG8H8_001303"
     CDS             81..512
                     /gene="mraZ"
                     /locus_tag="ACG8H8_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP8986388.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            533..1492
                     /gene="rsmH"
                     /locus_tag="ACG8H8_001304"
     CDS             533..1492
                     /gene="rsmH"
                     /locus_tag="ACG8H8_001304"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP8986389.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1497..1898
                     /gene="ftsL"
                     /locus_tag="ACG8H8_001305"
     CDS             1497..1898
                     /gene="ftsL"
                     /locus_tag="ACG8H8_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP8986390.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1899..4091
                     /locus_tag="ACG8H8_001306"
     CDS             1899..4091
                     /locus_tag="ACG8H8_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP8986391.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4117..5079
                     /gene="mraY"
                     /locus_tag="ACG8H8_001307"
     CDS             4117..5079
                     /gene="mraY"
                     /locus_tag="ACG8H8_001307"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP8986392.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5093..6454
                     /gene="murD"
                     /locus_tag="ACG8H8_001308"
     CDS             5093..6454
                     /gene="murD"
                     /locus_tag="ACG8H8_001308"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP8986393.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6480..7568
                     /gene="murG"
                     /locus_tag="ACG8H8_001309"
     CDS             6480..7568
                     /gene="murG"
                     /locus_tag="ACG8H8_001309"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP8986394.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7583..8803
                     /locus_tag="ACG8H8_001310"
     CDS             7583..8803
                     /locus_tag="ACG8H8_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP8986395.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8959..10284
                     /gene="ftsA"
                     /locus_tag="ACG8H8_001311"
     CDS             8959..10284
                     /gene="ftsA"
                     /locus_tag="ACG8H8_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP8986396.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10307..11548
                     /gene="ftsZ"
                     /locus_tag="ACG8H8_001312"
     CDS             10307..11548
                     /gene="ftsZ"
                     /locus_tag="ACG8H8_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP8986397.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11565..12242
                     /locus_tag="ACG8H8_001313"
     CDS             11565..12242
                     /locus_tag="ACG8H8_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP8986398.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12256..12855
                     /locus_tag="ACG8H8_001314"
     CDS             12256..12855
                     /locus_tag="ACG8H8_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP8986399.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12936..13157
                     /locus_tag="ACG8H8_001315"
     CDS             12936..13157
                     /locus_tag="ACG8H8_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP8986400.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13206..13988
                     /locus_tag="ACG8H8_001316"
     CDS             13206..13988
                     /locus_tag="ACG8H8_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP8986401.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14009..14716
                     /locus_tag="ACG8H8_001317"
     CDS             14009..14716
                     /locus_tag="ACG8H8_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP8986402.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14978..17776
                     /gene="ileS"
                     /locus_tag="ACG8H8_001318"
     CDS             14978..17776
                     /gene="ileS"
                     /locus_tag="ACG8H8_001318"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP8986403.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17825..19351)
                     /gene="zwf"
                     /locus_tag="ACG8H8_001319"
     CDS             complement(17825..19351)
                     /gene="zwf"
                     /locus_tag="ACG8H8_001319"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP8986404.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19366..20013)
                     /locus_tag="ACG8H8_001320"
     CDS             complement(19366..20013)
                     /locus_tag="ACG8H8_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP8986405.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6850 a         3502 c         4330 g         5488 t
ORIGIN      
        1 cggatttccc agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt
       61 ggaggaatgg ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag
      121 gacgtttaat cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac
      181 gcggattgga tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta
      241 aactgaacga aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt
      301 cggctgccac tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa
      361 gaaattatgc agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa
      421 tttgggatga gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa
      481 ttgctgaaag tatgattgat ttcggactat aagaatagga agaggctgac taatgactga
      541 aacatttcaa cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc
      601 ggatggcgtt tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag
      661 ccaactaagt tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca
      721 cgccaagctg cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa
      781 cttcagagaa ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta
      841 tgatctaggg gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca
      901 agatgcaccg ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat
      961 taatgaatat tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt
     1021 ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac
     1081 tggtgagctt gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg
     1141 gcatcctgca aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc
     1201 cgaagaggcc tctttagaac aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt
     1261 aatcacattc cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac
     1321 ggtacaagat ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa
     1381 agtcattact cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc
     1441 acgcagtgca aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg
     1501 cagaattaaa gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg
     1561 aacaacaaat gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa
     1621 aacgattaaa gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta
     1681 ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag
     1741 aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac
     1801 aagaaaaaaa cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat
     1861 tgtctattaa tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc
     1921 ggcggtttat ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca
     1981 ttttatttgc tacgagtatt ggattgttct ttttatttgc cgtccgtttc tcgtatatcg
     2041 ttattggagg tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag
     2101 gaagtgaagt agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg
     2161 cagaagatgc ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg
     2221 ataaaaaact gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga
     2281 atctttctat agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc
     2341 tgtatcaagt tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca
     2401 tcgaagcgga catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc
     2461 ggatgtatcc aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag
     2521 atgagaatgg cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg
     2581 gtaaagatgg taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag
     2641 tggcagaaga agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac
     2701 tacaaagtta tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat
     2761 tgacagcagt cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta
     2821 ctttcaatcc tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct
     2881 tggtacaaga tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta
     2941 tcgaagaagg agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag
     3001 atgctacgat caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc
     3061 tttcatggtc aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt
     3121 ataactattt acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag
     3181 taaacggtgc tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag
     3241 cggtaggtgt gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta
     3301 caatgattca acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa
     3361 caactcaaac agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg
     3421 aatatatgag agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg
     3481 tacctggata caatgtatcg gcaaaaaacg gaacagccca aatcgcttct gatacagggg
     3541 gatatcaaac aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag
     3601 atccagacta tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat
     3661 tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag
     3721 attcagatac agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag
     3781 cggacgttgc agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg
     3841 gtaaaaaagt acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac
     3901 tgatcctata tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag
     3961 atatcatgaa gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt
     4021 gtgtcaaaca agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt
     4081 tagaagaata agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt
     4141 cccattgtca gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt
     4201 caaatgaaaa agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa
     4261 gccggaactc ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt
     4321 tgggttggag cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta
     4381 gcgttatacg gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac
     4441 atgggactga actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat
     4501 cttgtttacc gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct
     4561 ttaggaatca tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta
     4621 aatctgacag atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct
     4681 tacggtctaa ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt
     4741 ttaggaggtt tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga
     4801 gatgtcggtt cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa
     4861 gaatggacat tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt
     4921 caagttactt cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat
     4981 cactttgaaa tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc
     5041 attgtgactt cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa
     5101 aatatcaaca tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag
     5161 tgcggccaaa ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt
     5221 tgacgaaaat ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag
     5281 tcatccaatc gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc
     5341 ctacagtcat cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt
     5401 tgagctagct tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa
     5461 aacaacgaca acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc
     5521 acgtttagcg ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga
     5581 agataaaatt gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc
     5641 tcatatagcg gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa
     5701 agaatatgtc aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat
     5761 ccttaactgg aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact
     5821 gcctttctcg acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata
     5881 ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt
     5941 agaaaatgca ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat
     6001 acgagaaacg ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat
     6061 ccaaggcaga aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc
     6121 tctaagtgga tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa
     6181 tacatttgat gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca
     6241 aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc
     6301 cgattctgta gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt
     6361 tttattatca ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa
     6421 cagattcatg gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa
     6481 tgaaaatttt ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg
     6541 tcaattatgt aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag
     6601 gtctagaaaa caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag
     6661 gatttaaacg aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta
     6721 tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag
     6781 gatatgtttc cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc
     6841 atgaacaaaa cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga
     6901 tcgctctttc atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag
     6961 gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg
     7021 atttggatcc agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa
     7081 tcaatcaagc agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt
     7141 atgcttcagg agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa
     7201 atattagtgt ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc
     7261 tggtggggcg tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag
     7321 tgttggttcc cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg
     7381 ttcacgcagg agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc
     7441 agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta
     7501 aagaacaagg aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca
     7561 aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc
     7621 gtcttctgat gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga
     7681 acaagagatg aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc
     7741 agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa
     7801 aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc
     7861 ttttttgaac cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt
     7921 agctttgatc atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc
     7981 ttatagcaaa ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat
     8041 catctctgat acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc
     8101 tacttatatc gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt
     8161 caaagggatg aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc
     8221 aaaaaacaat caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc
     8281 aaatcctaca aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga
     8341 attaacaaag caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa
     8401 gtacacgcca agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca
     8461 agttattgta aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa
     8521 agatatggat gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg
     8581 tagcactcaa gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa
     8641 tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg
     8701 atcgtctagc acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga
     8761 agataatcca agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa
     8821 gtaagaatgt actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat
     8881 ctattaataa gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag
     8941 taggagggaa tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg
     9001 acatctgtta aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta
     9061 ggaaacgcaa aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc
     9121 caagcgatac aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc
     9181 gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca
     9241 gttaacggag attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc
     9301 cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact
     9361 gtcgatgggt tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg
     9421 tacggtctgt tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa
     9481 aatgccggat taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt
     9541 ctttctgacg gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca
     9601 acaacggctg ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc
     9661 gaatttgtca ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg
     9721 ttgaaaataa actacgggga tgcataccca gaacggacat caccagatga agaatttcca
     9781 gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt
     9841 atttcagctc gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa
     9901 gcattagagt tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt
     9961 gtggacttgg cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg
    10021 gggctgagaa acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg
    10081 agtgaagtgt atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa
    10141 atggtagttg aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca
    10201 atctacgatg agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt
    10261 ttctttacaa atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat
    10321 tgataataat atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg
    10381 aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa
    10441 cacagacgtg caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa
    10501 atatactcgt ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga
    10561 aagtgaacaa tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg
    10621 aatgggtgga ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt
    10681 gggcgcattg actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg
    10741 ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat
    10801 ctcaaataac cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg
    10861 tgaagcagat aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc
    10921 aggttacgtt aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc
    10981 attgatgggg atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc
    11041 aatctcctct ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat
    11101 cacaggcggt ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa
    11161 tgcagcaact ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga
    11221 tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc
    11281 tagccgacca gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat
    11341 ggatcaagct aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag
    11401 acgtgaacaa aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa
    11461 agaatttgat acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac
    11521 gccaccgttt ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact
    11581 tgcagaaaat caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag
    11641 aagttacatt agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta
    11701 gagctggcgt tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag
    11761 atttatttga ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg
    11821 taaaattgat tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag
    11881 aagaaatcca gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg
    11941 gtgaggaatc aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag
    12001 ctgcctgcac aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg
    12061 atgagcaaat ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa
    12121 aaggttatgc ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa
    12181 ttgcgattga agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat
    12241 aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta
    12301 gctgatgagg aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag
    12361 ccaactaaaa aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact
    12421 catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct
    12481 gaaaagaaaa aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga
    12541 acagcttcaa gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg
    12601 gaaccaagag cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca
    12661 accttagtga attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta
    12721 acaggaacag tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt
    12781 tgtacaccga ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat
    12841 ttgtttgatc tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac
    12901 ttcaacgggc agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt
    12961 ttccaggcgg gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata
    13021 tcagtttgtt cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg
    13081 caatcctgat cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg
    13141 tccatttagt ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga
    13201 aaattttgga tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt
    13261 cggtaggcga ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc
    13321 ttgacccaag acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt
    13381 tcatgtttta tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt
    13441 attacgaacc tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca
    13501 aatttgccag cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac
    13561 gctcttactt tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg
    13621 aggtggatca ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg
    13681 aaccaatcaa atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa
    13741 cagttagttc catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc
    13801 ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac
    13861 ctgattttgt acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga
    13921 tccaagaact tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac
    13981 gtaaataaaa taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa
    14041 gacattcccg acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt
    14101 agttgttcgt gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa
    14161 acattctgaa gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat
    14221 catcgtagct caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt
    14281 gatcgttact tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc
    14341 acatcaatta acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga
    14401 aaaagcaaga cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca
    14461 tcaacgtatc agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga
    14521 tgaaatttta caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt
    14581 tttagcaaaa cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat
    14641 agaagcacct atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga
    14701 aaatgaaaac gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt
    14761 ggaagttcgg taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa
    14821 attagagtaa gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag
    14881 atgcaactaa atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt
    14941 ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca
    15001 attaggaaaa acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca
    15061 aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc
    15121 tagctttgtt cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc
    15181 attgaataaa atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc
    15241 accatatgtt ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa
    15301 aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc
    15361 actttcccaa gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg
    15421 ggaacatcct tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg
    15481 taaaatggct gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc
    15541 aagtgagtct tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat
    15601 ttttgtagct ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt
    15661 catttggacg acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa
    15721 atttacgtat gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct
    15781 agaaacagtc caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg
    15841 acaagaaatg gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat
    15901 tttaggcgat catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca
    15961 tggggaagat gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga
    16021 ctcaaaagga ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc
    16081 aaatcctatg atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt
    16141 tactcatagt tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc
    16201 acaatggttt gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt
    16261 agagtgggtt atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga
    16321 ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa
    16381 tggagaagca atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca
    16441 tggatcaatt atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca
    16501 cccaggttct cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga
    16561 ttctggttct tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga
    16621 tatgtattta gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag
    16681 tgtggctatc aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga
    16741 tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat
    16801 caaacaattt ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga
    16861 tgttcgtgtt tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa
    16921 tacgatgcgt ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc
    16981 gtacaatgat ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa
    17041 agaaatccga gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat
    17101 gaacttcttg acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta
    17161 tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc
    17221 tgtagctttg acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc
    17281 attcctgaaa gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt
    17341 tgcaaaccaa gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt
    17401 cttaaaagca ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa
    17461 actaactgtt tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat
    17521 tgcacaatta ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt
    17581 gccagataac gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga
    17641 aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca
    17701 tctatgcggt cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg
    17761 attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt
    17821 gttatcaatc gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt
    17881 cgaatccgtc tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt
    17941 agttgggaaa atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc
    18001 aagattgagc gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta
    18061 atagtaattt ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt
    18121 ccaatttgat tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag
    18181 aaaatccttc agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt
    18241 cacatggttc atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt
    18301 tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg
    18361 accaacggaa gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag
    18421 catccacttt gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag
    18481 catattgccc gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt
    18541 tcaatgcttt gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca
    18601 ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac
    18661 tatgatcgta gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga
    18721 tattgtcgat atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag
    18781 ctgagatgtt ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt
    18841 ctgggaatac ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga
    18901 acggtttttc tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca
    18961 aatgagaaac gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac
    19021 cgtttaattg atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg
    19081 ttgaatcatt tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt
    19141 catctgaagg attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc
    19201 tccagggacg gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt
    19261 aaaggcggaa caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata
    19321 tcgtaaacaa tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac
    19381 aaaaatcgtg ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt
    19441 gatcggtcct tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc
    19501 aatagtgact aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt
    19561 tgtaccgaca ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt
    19621 tttaggaatc gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg
    19681 atctgcaaga tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt
    19741 ccagctatag tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag
    19801 actgctagct ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc
    19861 tacaagctct tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc
    19921 tagactagat acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat
    19981 gattttcaaa taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa
    20041 ccatcattag taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca
    20101 aaggattttt agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt
    20161 cgtttataaa
//
LOCUS       DAZKAO010000045        19709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000045 DAZKAO010000000
VERSION     DAZKAO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.045"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..665
                     /locus_tag="ACG8H8_001321"
     CDS             18..665
                     /locus_tag="ACG8H8_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP8986406.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            749..1693
                     /locus_tag="ACG8H8_001322"
     CDS             749..1693
                     /locus_tag="ACG8H8_001322"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP8986407.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1828..3279
                     /locus_tag="ACG8H8_001323"
     CDS             1828..3279
                     /locus_tag="ACG8H8_001323"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP8986408.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3300..4136
                     /locus_tag="ACG8H8_001324"
     CDS             3300..4136
                     /locus_tag="ACG8H8_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8986409.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4136..4795
                     /locus_tag="ACG8H8_001325"
     CDS             4136..4795
                     /locus_tag="ACG8H8_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8986410.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4921..5610
                     /locus_tag="ACG8H8_001326"
     CDS             4921..5610
                     /locus_tag="ACG8H8_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986411.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5616..6572
                     /locus_tag="ACG8H8_001327"
     CDS             5616..6572
                     /locus_tag="ACG8H8_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP8986412.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6616..7128
                     /locus_tag="ACG8H8_001328"
     CDS             6616..7128
                     /locus_tag="ACG8H8_001328"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8986413.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7384..7932)
                     /locus_tag="ACG8H8_001329"
     CDS             complement(7384..7932)
                     /locus_tag="ACG8H8_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP8986414.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8021..9202)
                     /locus_tag="ACG8H8_001330"
     CDS             complement(8021..9202)
                     /locus_tag="ACG8H8_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP8986415.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9438..10946)
                     /locus_tag="ACG8H8_001331"
     CDS             complement(9438..10946)
                     /locus_tag="ACG8H8_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP8986416.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11105..11296
                     /locus_tag="ACG8H8_001332"
     CDS             11105..11296
                     /locus_tag="ACG8H8_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986417.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11499..12416
                     /locus_tag="ACG8H8_001333"
     CDS             11499..12416
                     /locus_tag="ACG8H8_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP8986418.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12409..13482
                     /locus_tag="ACG8H8_001334"
     CDS             12409..13482
                     /locus_tag="ACG8H8_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986419.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13630..14148
                     /locus_tag="ACG8H8_001335"
     CDS             13630..14148
                     /locus_tag="ACG8H8_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986420.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14129..14521
                     /locus_tag="ACG8H8_001336"
     CDS             14129..14521
                     /locus_tag="ACG8H8_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP8986421.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14597..15532
                     /locus_tag="ACG8H8_001337"
     CDS             14597..15532
                     /locus_tag="ACG8H8_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8986422.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15665..16444
                     /locus_tag="ACG8H8_001338"
     CDS             15665..16444
                     /locus_tag="ACG8H8_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP8986423.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGNNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16437..16754
                     /locus_tag="ACG8H8_001339"
     CDS             16437..16754
                     /locus_tag="ACG8H8_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP8986424.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16998..18608
                     /locus_tag="ACG8H8_001340"
     CDS             16998..18608
                     /locus_tag="ACG8H8_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8986425.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18765..19613
                     /locus_tag="ACG8H8_001341"
     CDS             18765..19613
                     /locus_tag="ACG8H8_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8986426.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6372 a         3520 c         3971 g         5846 t
ORIGIN      
        1 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
       61 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
      121 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
      181 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
      241 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
      301 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
      361 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
      421 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
      481 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
      541 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
      601 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
      661 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
      721 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
      781 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
      841 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
      901 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
      961 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
     1021 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
     1081 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
     1141 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
     1201 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
     1261 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
     1321 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
     1381 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
     1441 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
     1501 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
     1561 gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
     1621 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
     1681 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
     1741 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
     1801 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
     1861 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
     1921 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
     1981 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
     2041 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
     2101 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
     2161 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
     2221 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
     2281 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
     2341 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
     2401 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
     2461 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
     2521 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
     2581 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
     2641 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
     2701 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
     2761 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
     2821 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
     2881 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
     2941 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
     3001 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
     3061 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
     3121 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
     3181 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
     3241 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
     3301 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
     3361 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
     3421 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
     3481 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
     3541 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
     3601 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
     3661 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
     3721 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
     3781 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
     3841 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
     3901 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
     3961 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
     4021 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
     4081 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
     4141 ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
     4201 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg tagggttgct
     4261 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
     4321 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt ttgggttacc
     4381 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
     4441 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
     4501 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
     4561 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
     4621 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
     4681 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
     4741 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa tctgaaaaat
     4801 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
     4861 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
     4921 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
     4981 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
     5041 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
     5101 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
     5161 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
     5221 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
     5281 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
     5341 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
     5401 ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
     5461 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
     5521 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
     5581 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
     5641 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
     5701 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
     5761 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
     5821 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
     5881 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
     5941 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
     6001 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
     6061 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
     6121 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
     6181 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
     6241 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
     6301 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
     6361 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
     6421 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
     6481 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
     6541 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
     6601 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
     6661 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
     6721 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
     6781 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt ggatcccgtt
     6841 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
     6901 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
     6961 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
     7021 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
     7081 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
     7141 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
     7201 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
     7261 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
     7321 gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta ttattcgata
     7381 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
     7441 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
     7501 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
     7561 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
     7621 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
     7681 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
     7741 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
     7801 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
     7861 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
     7921 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
     7981 atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc aagccagtca
     8041 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
     8101 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
     8161 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
     8221 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
     8281 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
     8341 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
     8401 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
     8461 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
     8521 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
     8581 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
     8641 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
     8701 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
     8761 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt
     8821 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
     8881 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
     8941 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
     9001 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
     9061 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
     9121 tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa
     9181 taatacctaa taaataactc aaataatcac tcctattcca aattccgttt tattttacct
     9241 gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg
     9301 cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc
     9361 ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat
     9421 ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag
     9481 gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta
     9541 gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga
     9601 ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa
     9661 gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat
     9721 aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct
     9781 gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt
     9841 gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca
     9901 tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt
     9961 ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg
    10021 aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc
    10081 ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca
    10141 gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc
    10201 acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga
    10261 tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact
    10321 gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt
    10381 tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt
    10441 ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct
    10501 acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct
    10561 agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg
    10621 ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa
    10681 gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc
    10741 agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat
    10801 cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa ttctaaatat
    10861 aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt
    10921 tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc
    10981 atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg
    11041 tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg
    11101 ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt
    11161 taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct
    11221 aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga
    11281 tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat
    11341 ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg
    11401 agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta
    11461 ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg
    11521 caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca ggacaagaat
    11581 cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag
    11641 acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat
    11701 acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc
    11761 aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag
    11821 tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc
    11881 ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag
    11941 agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag
    12001 acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa
    12061 tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg
    12121 aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct
    12181 ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag
    12241 aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac
    12301 gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc
    12361 ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac
    12421 gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact
    12481 tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag
    12541 gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac
    12601 acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat
    12661 caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga
    12721 aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg
    12781 aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa
    12841 ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg
    12901 aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc
    12961 tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta
    13021 tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac
    13081 tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat
    13141 caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat
    13201 acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat
    13261 gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact
    13321 acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat
    13381 caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc
    13441 aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac
    13501 cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt
    13561 cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt
    13621 tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta
    13681 gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc tattttttta
    13741 tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg
    13801 atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact
    13861 gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg
    13921 ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt
    13981 gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt
    14041 gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa
    14101 cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat
    14161 gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa
    14221 ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca
    14281 aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga gtattttgaa
    14341 acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca
    14401 ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg
    14461 tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata
    14521 agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg
    14581 aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc
    14641 tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag
    14701 tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct
    14761 gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg
    14821 acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc
    14881 acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc
    14941 gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt
    15001 ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg
    15061 cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg
    15121 tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga
    15181 tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa
    15241 agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa
    15301 gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag
    15361 tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc
    15421 ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat
    15481 gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta
    15541 ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca
    15601 atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat
    15661 tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact
    15721 ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt
    15781 tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt
    15841 agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt
    15901 tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa
    15961 gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta
    16021 cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt
    16081 ctcaatgact gcatgggccg gtaacaatat gatcggtaat tttttcggct cggtgattcg
    16141 agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat
    16201 gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt
    16261 attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt
    16321 tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg
    16381 cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga
    16441 ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct
    16501 tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga
    16561 aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg
    16621 aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg
    16681 cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc
    16741 tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca
    16801 aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg
    16861 gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga
    16921 gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa
    16981 tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg
    17041 ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg
    17101 ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg
    17161 gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg
    17221 tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata
    17281 tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag
    17341 ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc
    17401 catctttgat tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg
    17461 atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct
    17521 tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga
    17581 tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg
    17641 gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa
    17701 gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg
    17761 gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact
    17821 ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca
    17881 atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct
    17941 tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag
    18001 atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag
    18061 ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa
    18121 tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat
    18181 ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca
    18241 tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct
    18301 tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag
    18361 cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc
    18421 ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa
    18481 gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt
    18541 ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca
    18601 aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta
    18661 cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag
    18721 ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag
    18781 ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta
    18841 ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg
    18901 atgaatatta tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg
    18961 taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa
    19021 gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg
    19081 acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac
    19141 acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg
    19201 cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat gatttgaaca
    19261 atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa
    19321 tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa
    19381 gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac
    19441 cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag
    19501 agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca
    19561 ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt
    19621 tattcaaaaa acataccaaa aatattggct ctttgtcaaa tgatgttggt aaagagaaat
    19681 cttagaataa tttggacaga acagaaaga
//
LOCUS       DAZKAO010000046        19333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000046 DAZKAO010000000
VERSION     DAZKAO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.046"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..709)
                     /locus_tag="ACG8H8_001342"
     CDS             complement(<1..709)
                     /locus_tag="ACG8H8_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8986427.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
     gene            975..1319
                     /locus_tag="ACG8H8_001343"
     CDS             975..1319
                     /locus_tag="ACG8H8_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP8986428.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1320..1739
                     /locus_tag="ACG8H8_001344"
                     /pseudo
     CDS             1320..1739
                     /locus_tag="ACG8H8_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1831..2181)
                     /locus_tag="ACG8H8_001345"
     CDS             complement(1831..2181)
                     /locus_tag="ACG8H8_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986429.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2147..3478)
                     /locus_tag="ACG8H8_001346"
     CDS             complement(2147..3478)
                     /locus_tag="ACG8H8_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP8986430.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3734..4300
                     /locus_tag="ACG8H8_001347"
     CDS             3734..4300
                     /locus_tag="ACG8H8_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8986431.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4445..4532
                     /locus_tag="ACG8H8_001348"
     tRNA            4445..4532
                     /locus_tag="ACG8H8_001348"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4477..4479,aa:Ser,seq:cga)
     gene            4866..5840
                     /gene="bsh"
                     /locus_tag="ACG8H8_001349"
     CDS             4866..5840
                     /gene="bsh"
                     /locus_tag="ACG8H8_001349"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP8986432.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6165..8060
                     /gene="asnB"
                     /locus_tag="ACG8H8_001350"
     CDS             6165..8060
                     /gene="asnB"
                     /locus_tag="ACG8H8_001350"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP8986433.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8255..8701)
                     /locus_tag="ACG8H8_001351"
     CDS             complement(8255..8701)
                     /locus_tag="ACG8H8_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP8986434.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8929..9078
                     /gene="rpmG"
                     /locus_tag="ACG8H8_001352"
     CDS             8929..9078
                     /gene="rpmG"
                     /locus_tag="ACG8H8_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8986435.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9166..9684
                     /locus_tag="ACG8H8_001353"
     CDS             9166..9684
                     /locus_tag="ACG8H8_001353"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP8986436.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9761..10717)
                     /locus_tag="ACG8H8_001354"
     CDS             complement(9761..10717)
                     /locus_tag="ACG8H8_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8986437.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10883..11689
                     /locus_tag="ACG8H8_001355"
     CDS             10883..11689
                     /locus_tag="ACG8H8_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986438.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11686..12675
                     /locus_tag="ACG8H8_001356"
     CDS             11686..12675
                     /locus_tag="ACG8H8_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP8986439.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12672..13676
                     /locus_tag="ACG8H8_001357"
     CDS             12672..13676
                     /locus_tag="ACG8H8_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP8986440.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13808..14350
                     /locus_tag="ACG8H8_001358"
     CDS             13808..14350
                     /locus_tag="ACG8H8_001358"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP8986441.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14370..15068
                     /locus_tag="ACG8H8_001359"
     CDS             14370..15068
                     /locus_tag="ACG8H8_001359"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP8986442.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15089..15301
                     /locus_tag="ACG8H8_001360"
     CDS             15089..15301
                     /locus_tag="ACG8H8_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP8986443.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15301..16263
                     /locus_tag="ACG8H8_001361"
     CDS             15301..16263
                     /locus_tag="ACG8H8_001361"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP8986444.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16281..16676
                     /locus_tag="ACG8H8_001362"
     CDS             16281..16676
                     /locus_tag="ACG8H8_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8986445.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16839..17204
                     /locus_tag="ACG8H8_001363"
     CDS             16839..17204
                     /locus_tag="ACG8H8_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP8986446.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17201..19012
                     /locus_tag="ACG8H8_001364"
     CDS             17201..19012
                     /locus_tag="ACG8H8_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP8986447.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19074..19241)
                     /locus_tag="ACG8H8_001365"
     CDS             complement(19074..19241)
                     /locus_tag="ACG8H8_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP8986448.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6186 a         3284 c         3963 g         5900 t
ORIGIN      
        1 cattactgat ctgtttccaa tttgaaaatt cctttccaca atcaaaagtg atggatttga
       61 atagattttt cggtacagat tcaaaccaat gatttaattt gttttcaata tcaatcgctt
      121 gtctaccaca cggtttcaat gtgatgataa cttttgataa tcgctcaact aaggtaatta
      181 cagcactttt atgtttcaaa cctacgatag tgtcaccttc aaggtgacca aactcatttg
      241 agaattggct ataatctttt tcacgttcat gaatagagcg acggaaagct tgttttccac
      301 gtctttcttg atgtccattt ggtttacgct tgcctttcat cggtaagtca gaaggattaa
      361 atagcccctt tttgaacata cgataaatgg tacgagcaga acaacgaata ggaaacgctg
      421 cacgaccaac aatcacatcg ggtgtccatc cttgaacaac ctttctttga atatattctg
      481 attgttcctc aggtaaaaca agcgggcgtc taccacagtt tgatttgttt ttcttatact
      541 gttgatagta ctctaaggct gattttcctt gcttgaagaa caggtatact ttatggatcg
      601 tttgtcttga acgattcaag aaatgcgcag ttttcgcaac agattgattt attttgaaat
      661 aagactctat cattacaagt tcatccgttg taagatgggt ataggtcatt tgtactcact
      721 ccttataatt ctttggacgg aactattttg agtgtagcac aaatgacttt tttagttgtc
      781 tagcttaatt ttacaatcgg cgaataaaaa aagcgttagc aactatgctt aaaaagcata
      841 tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat gaaatgcgaa
      901 gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac agtcgtgaag
      961 ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta ggagattttg
     1021 caccaaaatt tgcagaattg aatgatgatg ttttatttgg acaagtatgg tccagagaag
     1081 aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca ggaggaaatt
     1141 ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact agtgaagaaa
     1201 tagcagaaat cattacccat ttagcttttt atgtaggatg gccaaaagct tggtcagctt
     1261 ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa gaggcgtaaa
     1321 tgacgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg
     1381 aagcttacgc tcaagtattt accgggtaaa gctatctgaa tatgttagtg aatgatcccg
     1441 aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat cactggcatg
     1501 tccataaaga cggctatcaa atcttacttg tgaccggagg agaaggctgg tatcaggaag
     1561 aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac aaagggatta
     1621 aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc actgctggta
     1681 attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa aatcaataat
     1741 gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag ctgctttttt
     1801 gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag cacgtcgaaa
     1861 gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa tcaaactacc
     1921 taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt gataatagat
     1981 ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta aatcttctgg
     2041 ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa attctactcc
     2101 aatacgtatt tcagttattt catgggaata taaataagta agaagctcat cgatgattct
     2161 ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta aagttatcta
     2221 gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta tatagattta
     2281 gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagtaca ccagctatta
     2341 caccgtaaaa actgccgtaa aaaccgctta tgggcgcgag acctgtccca aacacagctg
     2401 caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa gcaataaata
     2461 ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg aaacccaccg
     2521 cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc aaaacataag
     2581 aagacagcat cagaccaact aagcctatgt tgatcaaagt agcaccaata ccagaattag
     2641 caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc
     2701 gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca aaaataatcc
     2761 aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga
     2821 ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat ccaatattat
     2881 aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga agaagtagac
     2941 caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga aaacgcatac
     3001 caaaagtgat ataactaacg accggtgata atgcgctacc aaacagtccg atcatgatgt
     3061 actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa atgattgaga
     3121 tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa
     3181 ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa agtaaggtca
     3241 aaatccctac atttatgaat gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa
     3301 gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta ggagtattag
     3361 aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt
     3421 tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg ttttccatta
     3481 cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg gacaactatt
     3541 atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat ttttttaatt
     3601 ttgcaaaaaa aagttaaaga agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa
     3661 tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc agtaaagaag
     3721 tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa aagctgctta
     3781 tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg ctaaaaagat
     3841 ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt taaaaaatac
     3901 gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg aacgtactgg
     3961 tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc atcatagtct
     4021 ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg atttttcata
     4081 caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat cggatgaata
     4141 tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt cattgatgtc
     4201 atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac ggacaattga
     4261 ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca gtatgaattt
     4321 gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa aaagcgattt
     4381 ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta aaaatagtac
     4441 ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa ccggctaata
     4501 ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa
     4561 gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct attttttcgt
     4621 ttgattgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaataagggg
     4681 ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac aatatttttt
     4741 gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt tggccttttt
     4801 ttgataaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt ttaggacggg
     4861 aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga aggaattttg
     4921 attatgagat gtcttataat gaagtggtca ctattactcc aagaaattat cgtttcgatt
     4981 ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca gctggggtat
     5041 ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg gcaggactga
     5101 attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac gttgcaccat
     5161 ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct aaagaattat
     5221 tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg tcacctttac
     5281 actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa gatggacttc
     5341 atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat tatcaattat
     5401 ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat ttttcaaacc
     5461 agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct ggagatctct
     5521 cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca tcaggtgatt
     5581 cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag caacaaaaag
     5641 gagtatgcga tactggagaa agaaaatacg aatatacgat ttattcttct tgctgtaacg
     5701 ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact gctgtaaata
     5761 tgaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata aaagagcaac
     5821 agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt ttctgataca
     5881 gtcataatta gacacgatct ttaggtcgtg tctttttttg ctggtttttt tggaaatagc
     5941 atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag aaaacatggt
     6001 aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt ttgttttcac
     6061 gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat aagatgcata
     6121 tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc ggtattgtag
     6181 gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg gatcgaatcg
     6241 ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct ttaggattca
     6301 gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat gaagatcgga
     6361 caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga gaagaattga
     6421 ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg catggttacg
     6481 aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt gctatttggg
     6541 ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa ccatattatt
     6601 atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt ctgcctcatc
     6661 ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact ttccaatatt
     6721 ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa ggccattatt
     6781 atacgtataa agatggaaaa atagacattc agcaatattg ggatgcagat tttgaaacaa
     6841 aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt caagcttcta
     6901 ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc ggggttgatt
     6961 ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg tttgatgata
     7021 aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac ttagataata
     7081 cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc cagtatcact
     7141 tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca aatcttgctt
     7201 cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt gcgggatacc
     7261 aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa ggattgaaaa
     7321 aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg aaaaatttcc
     7381 atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt atcggtcatg
     7441 ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat caaacagcac
     7501 catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc aaagatgaag
     7561 tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc ttgttaaagg
     7621 cagataaact ttctatggca agttctttgg aagtccgagt acctttattg gatatcgaag
     7681 tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac aacacaaaag
     7741 atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat cgtgtgaaat
     7801 taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac gaacaagtca
     7861 aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa atcatgaagt
     7921 tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg acaatcttca
     7981 gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa gcagaagcaa
     8041 tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt ttctcctacc
     8101 gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt ttatagatat
     8161 tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata agggggttgt
     8221 gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct ttttcttttg
     8281 tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact tgctttcctt
     8341 tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc cctttagatt
     8401 tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc agttttccag
     8461 cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt tcgaaagttc
     8521 gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg gcaattggga
     8581 tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg attgcttttt
     8641 ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg ttccatggca
     8701 tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa gggtatttat
     8761 acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt caagtagttt
     8821 tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta agataatcaa
     8881 gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat gcgcgtaaac
     8941 atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa aaacaaacgt
     9001 aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa agttactttg
     9061 caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca aagaattgag
     9121 acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt tatttccatg
     9181 tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg gcgattgcca
     9241 aatgatatgc gtttctttcg cgaacacaca atggatcgaa ttttagtgat gggaagaaag
     9301 acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt gttgacaacg
     9361 caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga tgaacttttg
     9421 tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg gatttttcaa
     9481 gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac gtttgaaggc
     9541 gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga gtatgagggg
     9601 ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag agtaaaaaat
     9661 agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa gaaaaaaagc
     9721 atctcacgac taatcgtgaa gatgcttttt ttagttaaaa tcaatttagt ttttctttga
     9781 tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta gattcaggtc
     9841 cgaattgcag tacatgattg tctgtcactg cttttagatt cttccagctt ggttcattga
     9901 acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct aattcagcta
     9961 gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc tcttccacta
    10021 gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac cgatttttgc
    10081 tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg ttctcgattt
    10141 tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa atgatttttt
    10201 ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct tcccatgtga
    10261 catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt tcgtatttcc
    10321 cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag cttaacactt
    10381 tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct tgcagataat
    10441 cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct agtgcaatca
    10501 gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc tctactttat
    10561 gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa ttggcagttg
    10621 tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc agcagcaata
    10681 tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta acataattga
    10741 gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg cttcatttgg
    10801 ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat tgagaatgat
    10861 tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg cagccggcta
    10921 cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga aaatcactag
    10981 tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta cccgtatact
    11041 acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat acagttcaaa
    11101 agaacttgca aaaaaaattg cattattagc acaaactagt gaacacccta caggtatgac
    11161 agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca gtggcatgga
    11221 cacgcaagat catgaagcaa ttgagtgggc gttggatgct acaggtctag ctggatttcg
    11281 tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga tcgctatggc
    11341 gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt tagatccggc
    11401 acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac aaacaacgat
    11461 cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt tattagcaat
    11521 gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa aagagtgtat
    11581 gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa ataaaccagt
    11641 ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag gactatgaag
    11701 aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt tgttgctctt
    11761 tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt tgattttgat
    11821 agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt ggcgggagca
    11881 tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt gacaagaaat
    11941 cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc aatggccctt
    12001 agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc attggcaggt
    12061 tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat gggcatgaat
    12121 cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc tatcagccaa
    12181 gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt aggcggagct
    12241 gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact tgctgttttg
    12301 gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac acatgcatta
    12361 gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt tgcagtgtta
    12421 gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt ggttccgcca
    12481 gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc ttttttagtt
    12541 ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc cccttttgaa
    12601 acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat acaagtaagg
    12661 agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt gtacttgtca
    12721 tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat cagctttttg
    12781 ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa tttcgagttc
    12841 ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt tatatcttgc
    12901 aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat gccggagcag
    12961 gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg tttctattgc
    13021 tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt gcggcctatg
    13081 atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc gttaatgccg
    13141 gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat tatggatttg
    13201 tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt tgggcattgc
    13261 ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg attgtattgc
    13321 tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa agtcaaaaaa
    13381 tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca ggtagtttat
    13441 cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa gaaaataact
    13501 ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca gatatggttg
    13561 ctagagtgat tctactaagt ggggaagtac ctacaggaat tttgattgcc ctttttggtg
    13621 cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac agttgaaatt
    13681 cattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt atgcatttat
    13741 ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga gaaggaaggg
    13801 tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa agctgaaaaa
    13861 tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac agctattcgc
    13921 cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg aatttgaact
    13981 ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag ttgtacctaa
    14041 aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg ttcttgtacg
    14101 ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg agatgatcga
    14161 tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg gttttggcgg
    14221 tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc ttgtttttcg
    14281 tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc aacaattgtt
    14341 tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata aaattgcgtg
    14401 catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca gcggtcttcc
    14461 tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg tatggtcctt
    14521 atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc cattttggac
    14581 tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa atcgaggcaa
    14641 tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg ggaaatacag
    14701 caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcaagtact tcgatattcg
    14761 gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca gcgatccagc
    14821 agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt cttctggata
    14881 cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg ttagggaatg
    14941 ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt ctttctacac
    15001 tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa tatggtttac
    15061 ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac agcagctgtt
    15121 caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg aattaatgac
    15181 tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat acatgacagc
    15241 gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag gaaatttgta
    15301 atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt tgcaatttta
    15361 acaccagaag gagagattca gcaaaaatgg agcatcgata ccaatatttt ggatgatggt
    15421 aagcatatcg ttcctgaaat cattgaatca atcaatcatc gtttgaattt atataatatg
    15481 aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga cagcgaagct
    15541 ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt gaaaaaactg
    15601 atcgaggcag gaacaggaat caaattcgct atcgataatg acgcgaatgt tgctgcttta
    15661 ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt tactctagga
    15721 acaggtgttg gcggaggtat cgttgctggc ggaaatttga ttcacggcgt gagcggagct
    15781 ggaggagaaa tcggacatat cactgtggat cctgaagggt tcgaatgtac ttgcggtaaa
    15841 cgcggatgtt tagaaactgt atctagcgct acaggtgtgg tacgcctagg tcgtttcttg
    15901 gcagaagaat acgcagggga ttcaaaactg aaagcaatgc tagacaacgg agaagaagta
    15961 acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt gatggttgtt
    16021 gatcgcgtat gcttctactt aggtttagct tgcggaaact tggggaatac attaaatccg
    16081 tcaagtatcg tgttaggtgg aggagtatcg gcagcaggag aatttttacg tagccgtgta
    16141 gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa aatcaaatta
    16201 gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt gaaatttgct
    16261 taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg ctattgattg
    16321 tgcttgcaat catcctttgg gaagtctatc tacgagtaat ggccaaacgt tcagcaacga
    16381 ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc gatgttcgcg
    16441 aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct tacactgttt
    16501 tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac gatcaaaaga
    16561 aaagtttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa gagatttatt
    16621 tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa gtataaaaca
    16681 gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc aagatcacat
    16741 gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat tatctacata
    16801 tataagctaa gcgaaaaggt agaaagaagg aaacaactat gatcaaatta aaacgtgttt
    16861 accttgatgc tgaaaaagag gatggctata gaatactcgt ggatagattc tggccacgag
    16921 gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca cctagtaatg
    16981 aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca aagaaatacc
    17041 agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc agtcaactta
    17101 tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat cagaaccatg
    17161 ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag ggatcattgg
    17221 tgccggacct agaggattaa gtgtagcaga aaggattctc cataataatc aggacagaca
    17281 ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt ggcggttgaa
    17341 tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt ttacagatga
    17401 aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat ggagtaaagg
    17461 acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa ttatagaaga
    17521 aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac tctatcaacg
    17581 ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg tttattcttt
    17641 agtaaaaaaa gtaagcaaaa gaaaagatgg ttatctttta cagacagatc agcaaagcta
    17701 ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga cagaagaaga
    17761 aagagtgtgg gtagactacg caaagaaaaa tgttttactg tatcagcttc cttctaatcc
    17821 tgcagatgta gatctttcga agataccgcc taaagaaaat gtctttttaa gaggtctagg
    17881 cttaagtttt tttgattatg tcggattgtt tacagtatca agaggagggc gcttcgagaa
    17941 cgaaaaggga aaactggtct atcatccatc aggcaaggaa ccgattgtat acagtggatc
    18001 aagaaggggg ctcccttatt accctcgagg aagaaatcaa aagcaaggcg gagcaatggc
    18061 atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg gatttttgac
    18121 tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt acaagaaaat
    18181 catcgaagaa tatcacttgc cgattccaaa gaaaaatttt gagcatgatt ttctctccct
    18241 ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat gggattggtc
    18301 gtatttagaa actcctcttg cctatcagaa tgagcccttt tcagaagcta gccgaatatt
    18361 tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat ttgcttcaac
    18421 atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat ggcacgtttt
    18481 ttctgctaag gaatacaaag aaatactatg gggatggttt actcatctaa atagcttttt
    18541 aacgatcggt ccgccaatca ttcggacgaa agaattagca gcactgatag atgcgggtat
    18601 ttttcatttg atcgaaccgc catttgatat tcagcaaaag cacaaccgct atgttatttc
    18661 tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc cacaaacaag
    18721 cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac ttgttcgccc
    18781 tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg ttgagcgagg
    18841 tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt gctatggaat
    18901 acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata cagatccatg
    18961 gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat aaaaatacgc
    19021 atgatgactt atctgttttt tagaaagtct tcatgcgtat ttttaaataa ttattaacga
    19081 gaaacgcgtt tacgcattgc ttttttacgg ttttcttcga tcattgcttc tttttcgtca
    19141 attggatcga tcactgtttt tttcacagaa gcaaccaaac cagcaacggc agcggctgtt
    19201 gccagacttc ctactaatat tccagataca aattttttca ttgaatccac ctcatttatt
    19261 atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg tttgtttgta
    19321 aacctttgca aaa
//
LOCUS       DAZKAO010000047        19050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000047 DAZKAO010000000
VERSION     DAZKAO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.047"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..264
                     /locus_tag="ACG8H8_001366"
     CDS             <1..264
                     /locus_tag="ACG8H8_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP8986449.1"
                     /translation="FVTFRRGGNCKGLDVLALNEDDLYDQTLTDNPIGFGLLPEDDEG
                     NEWFKMTLMNDNGDELSVEDTWSYLSDYIVSVEIIEFVADKEE"
     gene            283..573
                     /locus_tag="ACG8H8_001367"
     CDS             283..573
                     /locus_tag="ACG8H8_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986450.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIINEQKSCVLSIDSTLV"
     gene            765..998
                     /locus_tag="ACG8H8_001368"
     CDS             765..998
                     /locus_tag="ACG8H8_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986451.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            1065..1332
                     /locus_tag="ACG8H8_001369"
                     /pseudo
     CDS             1065..1332
                     /locus_tag="ACG8H8_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1418..2089
                     /locus_tag="ACG8H8_001370"
     CDS             1418..2089
                     /locus_tag="ACG8H8_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP8986452.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            2142..4118
                     /locus_tag="ACG8H8_001371"
     CDS             2142..4118
                     /locus_tag="ACG8H8_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8986453.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            4133..4885
                     /locus_tag="ACG8H8_001372"
     CDS             4133..4885
                     /locus_tag="ACG8H8_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8986454.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4901..5662
                     /locus_tag="ACG8H8_001373"
     CDS             4901..5662
                     /locus_tag="ACG8H8_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP8986455.1"
                     /translation="MRNRKWLFVCAAALFTFTAYTQLTVADSQESTDSTEQTIQTSDS
                     SAIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5675..5935
                     /locus_tag="ACG8H8_001374"
     CDS             5675..5935
                     /locus_tag="ACG8H8_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986456.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5932..8022
                     /locus_tag="ACG8H8_001375"
     CDS             5932..8022
                     /locus_tag="ACG8H8_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP8986457.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNLRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            8049..8513
                     /locus_tag="ACG8H8_001376"
     CDS             8049..8513
                     /locus_tag="ACG8H8_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986458.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8510..8752
                     /locus_tag="ACG8H8_001377"
     CDS             8510..8752
                     /locus_tag="ACG8H8_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP8986459.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8770..9072
                     /locus_tag="ACG8H8_001378"
     CDS             8770..9072
                     /locus_tag="ACG8H8_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986460.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            9142..11202
                     /locus_tag="ACG8H8_001379"
     CDS             9142..11202
                     /locus_tag="ACG8H8_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986461.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMN
                     NQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQFSQADSVATTASIKEEA
                     REQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSES
                     NEVK"
     gene            11202..13811
                     /locus_tag="ACG8H8_001380"
     CDS             11202..13811
                     /locus_tag="ACG8H8_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP8986462.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVKIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKREETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNLLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLH
                     DQLEEKNEENMFYKIVQSLENETAQKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIG
                     KRLLLEAEKAVKE"
     gene            13808..16339
                     /locus_tag="ACG8H8_001381"
     CDS             13808..16339
                     /locus_tag="ACG8H8_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP8986463.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            16389..16721
                     /locus_tag="ACG8H8_001382"
     CDS             16389..16721
                     /locus_tag="ACG8H8_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986464.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16722..17315
                     /locus_tag="ACG8H8_001383"
     CDS             16722..17315
                     /locus_tag="ACG8H8_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986465.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17478..>19050
                     /locus_tag="ACG8H8_001384"
     CDS             17478..>19050
                     /locus_tag="ACG8H8_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP8986466.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         7069 a         2954 c         3897 g         5130 t
ORIGIN      
        1 tttgtgacgt ttagaagagg cggaaattgt aaaggattag atgttttagc attaaacgag
       61 gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa
      121 gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta
      181 tctgttgaag atacttggtc atatttgagt gattatattg tcagcgttga aatcattgaa
      241 tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttatgaaatt tttcgaagta
      301 aaatcaccat actattatgc gttaatcgca gcaaagaatg aagaagaatg tgcagaattg
      361 tatgaacaag tagtaacaga tatcgaaaat cgtgaagaat ttattcgatg tttgcatgag
      421 ttaaatcgaa atgaagcaat tcaagaggat gcgaaaaaaa ttagtgaaga aacgttacaa
      481 ccaatcggta tggaggaagc tacaaaagac atttttacaa ttattaatga acaaaaatct
      541 tgtgtattaa gcattgattc tacattggtt taatagatcc aagattctat aatttttttc
      601 aaagtaaaaa gaaattaggt aatgattctc agaccaatta cttaggaaaa taaccataca
      661 aattagattt tacagataaa ggattagaag gaatcacggc gattaataaa aaggtagtat
      721 taccttaggc attgagtaag gaatttattt aggaggaaaa ttagatggcg cgaccagttt
      781 tagatgcgat ggatatggat actttgacaa aaggaaggta tgtacagctg ttagctcgtg
      841 gttattcacc taaagatgta ttcaaagccc tatctaaagg aacagatatg gaaaaagaaa
      901 gattacgaaa agaattcgac tattggagag agcataacgg aataaaagat ttaaaaccag
      961 cacgtccagt tattagacga aaaaaattga agaagtaaga ccaaagcgta tggcgtacat
     1021 aggctttttt attttgataa aaataatgaa aggaaggaaa actcatgaat tgtaaagaaa
     1081 tcgaaaatag aaaaaaagtg tcgaaagaaa tggaagaaaa gttactaaaa acaatgaaac
     1141 aaaaacaatg aaacaaaaac aatgaaacaa aaacacctta aaagattatc tgtcgcgcaa
     1201 tatatcaatg atatgcagtt aacaggaaaa gagaaagctt gtttgttagg ttcaatgaaa
     1261 aactttgaac agttgagacg aacttatgtg aaaaaaagtt ctaattgtca actattgtta
     1321 gaagtctcat gacattcatt aatagaattc atttttaaaa ctttttctat ggaaatgaga
     1381 aaaagttttt ttgttttgtc tgaaaggagg cagatggatg aaaaaatggt tatttggttt
     1441 tttaggagtc gctttaatcg tcttttgcag tgtgtttggc tatgttagct accaaaaaca
     1501 tgaaggagat gtgtttaagc aaaatataga aaaaaagatg cccgtagatc aaatcaatgc
     1561 acacgcaaag agttataagg aggatgcaac gaacgtcaat aatgatatgt ctttggggca
     1621 aatgttgtcc atacaaaaag aagcaatcga aatgggcgtc aataaacaag tatttgcaca
     1681 aattcagatt cctgcacttg gtttagcatt gccgattttt aaaggggcaa atcaatacac
     1741 tttgagtctt ggagcggcta cttattttta tgaggatgcc gaaatgggca aaggaaacta
     1801 tgttctagca ggacacaaca tggaaatgcc aggtgtttta ttttcagata ttcaaaagtt
     1861 gtcattaggg gaggtgatgg acttagttag taacgatggc gtctatcgtt acaaggtaac
     1921 gtgtaaattt atcgtatctg agtactttaa attaattgat ggcgttcctg aagagaactc
     1981 tttcttgtct ttaccaaaga aaggagagaa acccttatta acgttgttta cttgtgttta
     2041 tacgtcacaa ggaaaagaaa gatacgtggt acagggagag ctacaataac aaggttgtaa
     2101 tcaacgcgag tcgttgaaat atgaaatgag gagagtaaaa aatggcaata aatcggtatg
     2161 taaaaaaagc tggaatgctt gttggaatgt taggaatcat tgcaggctgc tttcagggat
     2221 atcgagcata tgcagaagat gtcacgcaaa aaacgccacc agagaaggtc aatatcacgg
     2281 ttcataaatt aatgtacgat caaggaacgc aattgaatgt tgatattgac gggatcaaaa
     2341 acgatggtta tacgcacgat caatatccta caggtgtaac gaaatacaac aaggcagatt
     2401 atggtgatgt tgagtttacg ttaggaaata ttacggatca agtattaccg actgaagatt
     2461 ctgatctaac caacgcgaaa gtggacgaaa tcgttaaaga tgtagaagac aagggatcta
     2521 acagtgagta cgtcaagaat gcaaccaata aaattacttc agctgtagat gaaaatggtg
     2581 agatcacttt tgctgatcaa ccagcctatg tcaattccaa agggaatgta tatgtggttt
     2641 atgaaagtaa atccgctgct ggattagtca cacaaaaagc aaaaccaatg gttgtgattg
     2701 cgccaatgac tgataataca ggttcaggtt ttttaaaaga cattcactta tatcctaaaa
     2761 atattgtttc taagttgagt tttgaattaa cgaaatttgg tgacgatggc acagctcaaa
     2821 gcaaacaaac acctttaaaa ggggcaaaat ttgagttata caagggcgaa cctggaaaag
     2881 gaacaaaatt aggagatctt gtatcggatg atcaaggaaa gctgactgct actgatttga
     2941 cgttaggaaa atactatttt gtggaagttc cttcagaagt tgtagtggga tcagataaag
     3001 aaccaacagc agatcaatat ttattaggcg cagacgctcg caacgatgcg cacaataaat
     3061 taacctttga aattacgaat gatggcgtga caagtgactt aaaagcttca tacgtaaact
     3121 ataaagcacc agtgatcgat aaaacagtaa caaatggaac aggacaagaa cattctttcc
     3181 aaattggtga cgcagtaaat tatcaaggaa cgattcatat cccaacagac attgcaggcg
     3241 gagcagacgg tatcacagta aacggagtga aaagcgaaac ttctccttac agtgtattta
     3301 aatgggggga tacagcagga caaggacttt cttatgtcgc tgctaaagca aatatcaaag
     3361 taacaaataa agatggatct gtggtattaa aagaaaatac agattataaa atccaaaatt
     3421 cagaaaatgg ctttgtgatc gattttatcg tgaataatgg ccaagtaagt gatactgtag
     3481 caagtttaca cggacaagat cttcaaatga cttataacat gtatgtaaat gatagcgcag
     3541 ctgttgccaa tccattaaca aatagcgtgg atttcgttta taacaacaat ccatttaatc
     3601 aggaagaaca ccacgaaaaa acgaaagctg acgttgtgac ttatggtgca aaattcttaa
     3661 aagtcgatag cggactattt ggaacaggaa tcaaagcaac accgcttgaa ggcgcagaat
     3721 ttgctgctaa aaacgcagaa ggaaaatatt atggtggatt agttgatacg gataaagacg
     3781 gtgtcaaaga agcagtatgg gtagacgatg tagcaaatgc agcgatccta aaatcagata
     3841 aagaaggtca ctttgaaatc actcgtttaa ctgaaggtga atattcattg gaagaaacaa
     3901 aagcgcctga aaactatcaa aaattaacga aagagatctc tttcaaagta gacaaagatt
     3961 catacaaaga agaaaaccga atcacgatca aaaacaatca aaaagtttcc gtaccaatga
     4021 ctggtagcaa cggttttcaa acgtatgtat taatctcttg cttgttgcta ggagcagggg
     4081 cactttctgc agttgtttac tttaagaaga aagcctagaa ggatcaaaac caatgattat
     4141 caggaagcct aagaaaaagc gaatcatggg taaatggatc attgctttct ggttactttc
     4201 agctgtaggt gtcttacttt tgatgcctgc tgaagcgagc gtggcgaaat accaacaaaa
     4261 tcagcagatt gccgcaatcg atcgaacagg aacggcagca gaaacggatt cttctttgga
     4321 tgttgctaag attgagttag gcgatcctgt tggtattctt acaattccaa gtatttcttt
     4381 aaaactacca atttatgacg gaacgtccga caaaatttta gaaaatggtg taggtattac
     4441 ggaaggaact ggtgatatta caggaggaaa cggtaaaaat cctttaatcg caggtcatag
     4501 tggactttat aaagataatc tttttgatga tttaccttca gtaaaaaagg gagaaaagtt
     4561 ttatatcaaa gttgatggtg agcagcacgc ttatcaaatc gatcggattg aggaagtgca
     4621 aaaagacgaa ctacaaagaa actttgtcac ttacttagaa ccaaatccca atgaagatcg
     4681 agtaacgcta atgacatgta cgcctaaggg gatcaacaca caccgttttc tagtgtatgg
     4741 gaagcgagta acctttacaa aatcagaatt aaaagatgaa gaaaataaga aacaaaaatt
     4801 atcatggaaa tggcttttag gaagtactgt ttttctttca gtcatgatca ttggttcgct
     4861 atttgtttat aaaaagaaaa aataaaggag ggtaaccaac atgagaaata gaaaatggct
     4921 atttgtttgt gcagccgctc tttttacttt cactgcctac acacagttga cggttgcaga
     4981 cagtcaagaa agcacggata gcacagaaca aacaatccaa acgtcagata gcagcgcgat
     5041 tcaaagcagc gatgaaaccg tcgattcgtc aacagttgat tcttcagaag aacctaaaat
     5101 tgctgaagac gatcaaatag tcaaacagac cattcacgtt caaaaaatta tttcttccga
     5161 acaattagac aaccaggggc aaaagcttga aaaacaagaa ggagtaaatg gcgcgaagtt
     5221 ttctgtttat gatgtatcgg acattttaca aaagatggat gttaaggatc tcactactga
     5281 ccaaattgaa tcccaattaa aagatcgagt gaaaaaacta tccagtgatc aactgaagct
     5341 aatttctaat ggagaaacaa agacaattga tcaacaaaca ggtgtctttg aattctcggt
     5401 agaagtacag gcaaatcaga aacaagctta ttatattgtc aatgaatcaa gtcctgaaaa
     5461 catttctaat agcgaggata ttttgctatt aacacctgtt tctgataaaa atggtgaatt
     5521 cttaaaggat gtgtggattt atcctaaatc agaagcttcg caacctaaag aagaagtaaa
     5581 aaaaatcgta tctactggcg taaagaaaaa cttctttgag aattgttggg attttgttac
     5641 tcacttgttt tctagagatt aaggaagaaa taagatgaaa ctgatcaaat tattattatt
     5701 caatctttct tgtttaaccg cagtcttttt gtactgcggt ttattatttt ctcaactatc
     5761 tattttgcca agctaccaac tatacctaat gaggatttta aacataccag ctgcttccat
     5821 gccgctagat attatggaaa agctcattag gaatccatta tttactacga tcaatttgat
     5881 cgttattgtc ggaatttcac tagtcattta taaaaaagag gggatgaagg gatgaatttt
     5941 aaacactcgt ggacaagacg gttaaaatat gtttttccca taatgatgat gttaggtaca
     6001 ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag ttattgtcga tccgtcaaat
     6061 ccaatccaga acgtaaagaa taattttatt attccaaaag atgttcctaa tgcaaaaaca
     6121 aatattactg taaacggagc gtcaaaagtt tacaaatatc atgttgatgc caacaaaggt
     6181 ggttttacag acgactacgt aggattagtg gagggaaaca aaaatatacg ctatccttct
     6241 ttccaaaagg aatacggtga aacaaacctt gttttagaag gcgtaacaac aaacaccaaa
     6301 gtcaatattg actatggaaa aatcggtacg tataacggga aaaaagtcaa tatcaagcta
     6361 gttttatcta atattcactt atactcggat acgttgccgt ggaatctact agataataac
     6421 tatacaaaaa ctcattttcg agacgatggc tataaaaata cgaatggtgc aatgagtaaa
     6481 tctaaaaaaa gaacagtact ttggatctca gacaatcttt tctcaggaat tgtttaccat
     6541 tcaacacaaa tgaatgtgca gctagtagcc acatatgagg acggtagccc cgttcaattt
     6601 tcaggagata catttatcag ttttaactct ttgaaccctg caggaggaaa aagcacggat
     6661 cttaaaggag aatataccca ctatgacaaa atgaatacaa cagattggta tgtcagaaaa
     6721 gatacggttc tttcagaatt taaatctttc tacaacaatc tgaacgtggt aggtggccac
     6781 ccaggaggaa gctctaagct gacacaagcg gataatgatt tcaataattt gcatgataaa
     6841 ttaggagatc cgaagtttgg tcaaggtact gtgtccttta aaatatctga agcaaatccg
     6901 acgtttgtga ttggttcttc taatgttcaa acctggttta ctttatcttc tgcgacaatc
     6961 tttagtgttg ttcctgatca acctgaaaaa acaggggttg ataaaaatgg aaataacgtg
     7021 aacgataaca tgttgcaagt aggagacacg attcaatatc gaatcaaaca gaaggtgaat
     7081 cgattgggag tggatttact agcggtttac gatcgatttg agttaatcga taatttacca
     7141 aaagaagtag attacgtcga tgcacacgtt gaaagtggta caaataaaaa attcgatgta
     7201 tctggtgaag tgacctatga caaaacaaaa caccaggtaa aatatgcagc gaaagctgat
     7261 acattaaaaa atcgaatgaa gtataacggc gaaacttacg aattagtgat caatgtaaaa
     7321 gtcaatgaat tggccaatca aaatagtgta gccaaaaatc agggaacgtc gatcattaat
     7381 aaagtagaaa aggatacaaa tatcattacc gtttatttcc ctaaaattcc agtgaaagaa
     7441 gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg gtaagaaggg tacgccgaca
     7501 gctcctttaa acgcaggatc tgaagtacaa tatctagtca cacaaaaatg gcacacaaaa
     7561 ggcgtagacg ctgcgtcaga tcactacaaa caatttagta ttcaagatcc aattgaagct
     7621 cgtttgacat ataaagaagt ttctgcgcaa gtaatagata agagtaaagg aaaagatatt
     7681 acttctgaag gcacactaac ctatgatagc aatttaagaa cgctgaagtg ggaagcttcc
     7741 gctgacttct taagtaacaa tcttttagat ggacgagaaa ttcagctgat atttacagct
     7801 aaaactccat tacaatcaga aaaaaatatt gataaccaag ccgtagtagc agtagataat
     7861 gtttctaata aaacaaatgt tgtgacaatt ggggttgatc ctaacttgcc acaggtcatt
     7921 gttcctaaaa caggttctac acatttagta acgatttcag cggtcagctt actattactt
     7981 gtgttagcaa cttttagtta tgcggttctg agatacattt aaaataaaaa aacgaaagaa
     8041 tgaataaaat gaaaaagtta ataatagtag ttacttcctt gattgccatg attgttattt
     8101 tagtcggatg tagtaatgaa aaaaataaac aaactaatca agataatggc gtattgaaat
     8161 caggaactat gtggaaagaa gaagtaggtg gtcttgttta taatttgaaa attatagacg
     8221 agactacctg ggaatactct gagagtgtat ggcatccaga tcctgtacaa ataacagtca
     8281 agaggcaaaa agactataaa ggtttagaaa gatacaaaat agtagattca gctggtgtta
     8341 gggaatttat aaacaaatct gattctttat ttattgttgt tccctatgag aagaatggcg
     8401 taaaaaaaat aatttttctt ggatctagca aggatgaaaa gcgaacgaaa gagaaattga
     8461 ttcacgatgg atcacaatcc aataaatata agttacaaaa gactagcgag tgattgctag
     8521 tctttttatt tgtcctgaag gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa
     8581 agccgcgtct aaaatcttag aacgccgcgg tgtatctgaa acacagtgga aacaagaagt
     8641 ccttgaaaaa gcacaatttg acctattacg gggaaaagac aaggacttag aaggatatat
     8701 ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt attaaacact aaaaggagcg
     8761 aacaatttta tgtttgaatc aattacaatg aattttttaa cacaaccgaa agtagtagct
     8821 gcggttgata tttttagagc attacaaact gccgcaacta gtttaaaaaa caatctgatg
     8881 atttttgctg ctgctgcggc cgttgcttcc attgttattg ggggtctttt cttcctattt
     8941 ggacgaagag gagctgaaaa tgggaagatg cacgtaggct atgtattgat gggggtagga
     9001 gttattttag cagcagcttc tattgtggtt tcgttatttg gattgtttgg acaaacacct
     9061 caaggattat aagaaagtaa aaaagtagga gtaagaagta gtcgatactt tttactcctt
     9121 ttttatgaaa ggagaatact catgtacttt acgattgcag gcaataccaa aacagataaa
     9181 gagttaataa aaaaagcaac gaaggaagta ggaaagcgta cttctttttt accgaaaaca
     9241 attaaatatt ctgtcattga aacggaagaa cagctacatg tgttagggaa agtactggaa
     9301 cacgaaagtc gatcttctgt taagttgact ctcacgttac atgacacaga aggaaaaata
     9361 gagaaagctg gtacagaata cgtttttggc ccactggatt atcaaatttc agaaggtttc
     9421 tatcctacat tgatggattt aatccgcctt gatgtgttta aagaaacgga tgaaactatg
     9481 agtgtggaag agtcttatcg aaacgaaaaa cttttacaag ttttagaaga agagttagag
     9541 agcgaccaaa gaaaagtgaa tcaagggagt gaagtcgaag taaatgaaga agtaccatgg
     9601 gaacgaccaa tatttccaaa gaaggagtca aaagaaaaag agacagtcta tgttgacgaa
     9661 caagctgata ttccgactca gaaatcgata tttgaggaag agaacgatgt gatgggtcaa
     9721 ttggaggtag atccctttga agaaacaatg gaggatgaag agacggccga agtagaagtt
     9781 gatccagttg aagctaatga agagtcaaca tggactgaag gggattctca ggaatatatt
     9841 gaaccactta tcagtgaaga agtagaaaaa aacgaaatag agatacaaaa ggcaactgta
     9901 tttgattata ttttggatca atatccgcca gagcatgaat ggatgaaaga aaaaaacaaa
     9961 ttatttttaa atgagcttta ttcgaaatat caattacccc aaacctatca agcattttta
    10021 gagagtaaag aagaattaat ctcacagggt aaaggcacac ttttagaaca gttagagtct
    10081 gtccaatcaa caaattggca tgagcttgca caagaagatt tattagccac ctttgaagaa
    10141 cgtcaacaag aaacagatca agaaattaat gagtatgcga cggaacagtc gtctaattgg
    10201 gagaaagcaa aagaggaact aaataaagaa gaacagcgag tgattgaaga agaagtacaa
    10261 aaaattaaag agcgatacga aaagaaacgc gacaatgcgt attccttgtg tgtagcaaag
    10321 atcaataatt ttactcagac gaatcaagaa gcgttatcga aagaaaaaga aaacttgtta
    10381 aaagaacgta ttaaagaacg aaaagaagaa ttttaccgag gtttgcgtac agataagcta
    10441 aagattagca aagaattaaa tgaacaactg aataagctat atcaaacaac gactgaagtt
    10501 cttatggaaa aacacgaaca gatccagcaa gaattacaag tacaaattcc aaaatggaaa
    10561 aaagagtatg aaattcaaca acaagaattg aaagaacaag aagaaaaaca acggcaaaaa
    10621 gaaaaagagc gcgaacaatt agaactaaaa aaacgtgaac aggatcttgc ggaacaacaa
    10681 atggcaatag aaaaacaacg gttaaaccaa gaaaaggaaa aagaagctgc tcgtaaggaa
    10741 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
    10801 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
    10861 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    10921 ctaggtggag ggacagtatt tgcttttaat catatcaatc aattttcaca ggctgattct
    10981 gtagccacaa cggcgtcaat caaggaagaa gcccgagaac aagcaagaaa agagtatgaa
    11041 caacaattgg aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagtatggaa
    11101 aatagcaaag agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    11161 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    11221 cgagacgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    11281 gctatactcc ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga
    11341 ttttttatag tggattagct tatcttccct taaactatgt tgttacaaga ataccagaag
    11401 catttcatcg atttgagatt attccaggaa gtttacaagg agtgaaaatt tttcagttac
    11461 aagaaatgcc gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa
    11521 caacaagttt aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctatactta
    11581 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tgggcaaaaa ggagataacc
    11641 gattaaaaac gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggag
    11701 gttttcctgg ttatggcgga ataccaatta ctcattataa aaagtactgg tatattcaga
    11761 cggatacagt aaatacttgt attattggta cttcccgttc agggaaagga cagatagaag
    11821 ttttagcaac cattgacaat ttgtctcgtg cagaaagaca aagttcaatg gttgttaatg
    11881 atccaaaaac agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg
    11941 tgtatgcatt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    12001 tcgtgaaata ctggaagcga ggagacatag atactgcaac gcagttaacc aatactttta
    12061 cgaatacgat ttattatgat gccaatgctt cagacaacaa atactttaac gaaaatgcgc
    12121 agaaagctgt aaatgcgctt atttttgcgt tgttagaaca agccgatcaa acaggggatt
    12181 actccaaatt aacacccaat aatcttgttg agcttctttc tgaaattggc ggttttaatt
    12241 atcaagatcc tgagaatgat tacaaacaac tcaatgcctt agatgaattc atgaatcagt
    12301 taccacctgg aaacatagca aaaaaacaat atggggcaac aaaaattggt tctgataaag
    12361 caaaagggaa tattttatcg acggcaatca caggattgaa tccatttacc ctaacgaaaa
    12421 ttgcgaaaat gacttctcaa tcaaccattg attataagtc cattggtttt ccgaagtatt
    12481 tggatctcaa actacacgag agtctgttaa atcagcgaat tgttgtagag ttttatcatc
    12541 aagggaaaaa atttcatgag gaacgtgtca aagtaggtta tagaggtttt tgtgaaatca
    12601 actttgattg tcatttagag cagggagatc atgtttgctt aaaatacgat catcaaggaa
    12661 aagaatatgt tgcttggtat gagttttctc aaagagtatt gaaagacgaa gagggaaata
    12721 tcgtttatcg aaaaaaacga gaagaaaccc atttaccaga attagaaaaa gaagctgttc
    12781 taacattaga tgaggataaa agtaacttat tagtagagtc tattcggatg cactataccg
    12841 ataaaccaat ggctattttt atgctcacac ctgattatga tccatctaac catatcattg
    12901 ttagtatttt tcttagtcag ttgtataaag aattatctac gcaatgtgtt aagactaaag
    12961 gagataaatg tcaccgtagg attcatttta ttttagatga atttgggaat atgccaccaa
    13021 tggataatat ggaaggaatc atgacagtca cagctggtag aaacatgttg tgggatctgt
    13081 ttattcaatc ctatcagcaa ctttatgcga agtatgggga acaaaacgca gcgattatca
    13141 aagaaaattg tcagacacat atttatatta tgtcgacaga cgataatacc atcgaagaat
    13201 tttccaaaaa agtggggaat aagacggttg aacaagagaa cagtaccacc aatttattag
    13261 gtatgaatac tcatatcaat cgcaatgttg attcagatcg tatcttaact cctgaaagaa
    13321 tttctacttt tttagaaggt gaaacgattg tactcgctcc tttaaaacga caagatttga
    13381 aaggacataa aatacggccg tatcctattt ttaatcatgg ggaaacgaat cttccttatg
    13441 cttatcaata tattgaagat ttcaatccat cgtgggatat caatgatata gacatacatt
    13501 gtacacatgc ggacttggat cttaaggctt tacaaattga ttatgattat ttcctaccag
    13561 atgaatatgc tagaaatctc tatcatggac tgcatgatca attagaagaa aaaaacgaag
    13621 aaaatatgtt ttataaaatc gttcaatctc ttgaaaatga gacagcccaa aaattattaa
    13681 ttcaatttta cgaagaacaa gcaacggatc gtcttcaaga atatgttgaa aagttgatca
    13741 aaaatgaaga aatttctgaa atgattggga aacggttgtt attggaagct gaaaaggcgg
    13801 tgaaagaatg atcattggag gaattttttc tggcatagcc gactggtggt caggcttatt
    13861 cggagaaggg gatgcaaacc ccaaaaccgt aacgaaagtc ttacaagatt atggtaatta
    13921 tctgcagtat caagatgtat ttagttggat ttggaacact tttctgtggg gattgattaa
    13981 aggattatat aatctcaata ctttactaga aaaaaccatt tatcaaagct ttgatttaaa
    14041 agacttattg aacgccgctg gggtaaatgc actatttcaa agtttgatta gtaaggtatt
    14101 ggctcttttt tgtgtcgtca cattaattta tttaggcttg aaatttttta tgagtaaaca
    14161 cccaccaaaa gtcaaaaata tcttggttaa tttatttatg gccatgatct tgatttttgg
    14221 tgggagttct ttaatcgatg aagggttaaa tatcagtaaa agcttttacg gagacgtcac
    14281 tgcagcaaat aaagcagata aaacgggttc acctgctttt caaattattc aaaataatgt
    14341 gatcgatgtc agtacattac tagcaactga cccgaataaa gtagaatccc tgccaacaga
    14401 caaaagaaat gctttaacag cgaaaaattt taaaacagcg aatatcaatg ggattattac
    14461 tcctaaacaa gctaaagaaa tggcggataa agataagagt ggtttatcag atttacaaaa
    14521 ggatcggtta agagcgttac aataccgatt ggaactagat gataatggga aagaaattcc
    14581 tgttcaaatt agtgacgaag gcttagcaaa atatgtctat acttcagggt atcgtcgata
    14641 cagtagtaaa ccaggtatta tcttagctgg tgaatcttct ttggcggtgg cctatttatt
    14701 tatcctattt acgatcgtta cttgcattat tgagctggta tttaaaaaat tctatttagt
    14761 gattgccgcg tcaacggact ttgagacagg ccaacggatg aaaagtgcga tagaagatat
    14821 ttctcaatcc ttcttgttat tggcgtttac gattttagaa ttacgaatct atacattaat
    14881 gctttcaggt atcggtgatt tgcatgcggc aggaaagatc aatggattct tgtatgttgt
    14941 tgctttaatt gtgctaacta ttgcgctatt caaaggctct caatcggtta cgaagatttt
    15001 cggtgttgat acgagtctga aaaacggcag taattctctt cttagcatgt ttgccttagg
    15061 aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg gtaagagtgg
    15121 tttaagccat ttaaatgata ttcgtaaaaa tggatttggc catcgaaaag atgttacccc
    15181 tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata gtaagaaaac
    15241 agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag aaggtcttgg
    15301 ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa aagccaaaga
    15361 caccttagaa gagaaattag acggcacgag agctaaagaa ttggctgaag ctgtgaagaa
    15421 tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gaagtcaaag aaggactaaa
    15481 agatgcgtat ggtgaaggac aagttaatgc agcagtaaaa gctaatacaa caaccgaaaa
    15541 agaaaatcaa gaaggtatag tgattccttc agtcaaagat caccaagaag cgttgcactc
    15601 taatgaaaat tcgattgaag agaaaaattc catagaagaa agaaattcag aagaaggaaa
    15661 aaatggacag ttaccaaaaa ctgatccaga acaatatcgc tataaaccta acagtgacct
    15721 tgaaaatgag aagcgaattt cagatttatc aaaagtaaat caagaagaca aagcagaaaa
    15781 tgagggacag ttaccaaaaa ctgatccaga acaatatcgg tatagatctg ttaactccga
    15841 aaatgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa cggatgagga
    15901 ttccatcgtt ctgccaaatg ataatggaat cgaagttgga actgaaaaat caacgaaaat
    15961 tggtttacct aaggcacaaa atttaccaat gaaagatagt cgtttgaatc aattcaagga
    16021 gaatgatcaa agctttgttc accaatcaac tgcaggtact gttcaaatga aaccaagtcg
    16081 tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttatctgaaa gtggtgagac
    16141 agggaatgcg tccgttcaaa atgttgtgac aagagaagct gcaccaaaac ctattcaaaa
    16201 cgttcataca ggttacaagc cagcaaatgt ggcatttact gcaccaacaa aaataaatgt
    16261 gacaagagaa actaaaaaca ggaacaatgc attgaaagaa caactattga gggatatcaa
    16321 acaggatatc aaaccataaa aggcctacaa gttaggcctt ttatttatat gaaaaggaga
    16381 aatcaattat ggataaatat ataaacatga aaaaccctcc aagatttagt ggagaatgga
    16441 agtttttcgc ttttttcttt ttagatctat taacgcttgg tggctgcttt ttttgggcga
    16501 aaatggtcaa taatcttgtc cacttaccac tcttttttgc actcttgaat tatggcttat
    16561 ggattgcgat agcgttagtg attgtttggc gaccctataa taacccaggt caacgacaaa
    16621 tttttgcgat tatcgagtct attttttttg cggacaagaa ccattatcat gcgttagatc
    16681 ctaaccgaaa ttatcatggt acgtataagg aggatgaata aatgcgagtc aaaggacaaa
    16741 attcagttga aaggaaatcc attttgcagt ttggaaaacc agaagagcgc tctatcttgg
    16801 acttatttcc gtataaatcc tgtacagaag agggagtctt aattactaga gaagatcatt
    16861 ttcaacgtta ttatcgtgta gtttctacag acgtggaagg attaaatgaa caagagaaac
    16921 tagaacgaat gaatcagttg accaccgtca tgcgtacgta tgtgccaaca attaagttag
    16981 ttagtttgac cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg caattattag
    17041 ataaaaatcg tatggaacag tccttaggaa gaaatctaca acgattaaaa aaatatgaaa
    17101 agaaattagt cgaagaaatc caggaattga aacgagcaga acaagaacgt cctgacttaa
    17161 gcttcttttt cgtcattgaa ggaaagacaa tcaaggattt acaaaccaga aaccgtcagt
    17221 taatgcgatc tagtggcatt ttagggttaa aaccactcta caaaaaagaa ttgatcaaga
    17281 ttctgtatcg aatgaacaat atgaatgatg agtaggaatg gagaagagag aatgaaatat
    17341 ccgttgatat ggttagaaaa agatgttccg aaggaagaga ccactagaaa atttcctcac
    17401 actttaagcc tgggtaagtt gggaaaaggg caatctttta accaagtaaa gaatcaaaca
    17461 agaagaggtg actgtacatg ttagaagcaa ttaaaacagc tttttcacca caaaatgatc
    17521 aaaaagtact tgatccatta aagcaactag gaaatcggga aatagaaatt ttaaaatcca
    17581 aagggtatga tttagatttt ttaaaacaag tgatgccaca aggtgggtta aattttgatg
    17641 aagaatatgt gacgtatggt aatggttatt cacgatgtgt gacagtttat cactacacaa
    17701 aaaatcctac gcttttttgg ttggccaatc tcatgaacaa tcctcataca gtggccacac
    17761 tcgatgttag cacagatgaa aagcaaaaag tgattgatca aatcaatcgt gccttagatg
    17821 aattaaatga tcgatcagat aatgaacgaa aagcgactga ccgaaataaa agcgcagaag
    17881 aatacctgga actagcggaa tatgctcgta aattgacttc taatggggaa atttctaagc
    17941 aagtcaaagt gagagtacat gtttacggaa acacgattca agaagtagat gaacgagcgc
    18001 aggaactgat cgaaaatcta aaagggatgg attatcgtgc ggtgacttat ctgcataaaa
    18061 ccaaacgaga ttggcaatcg atgtttacca tgttcgatga acaagctgaa tggttcggaa
    18121 atatcaaagg gcaatcacta ccatcgttaa acattggcgg aggtttcccg ttccaccacc
    18181 aaacgctaaa agatccagga gggacacaat atggggaaac catgacaggc ggcccatttg
    18241 tctggaatgc gacgtataaa aatagtgtac ggttgtctac taataccttg cttctaggaa
    18301 tgatgggagc tggtaaatcg accgttctta aaatgatcgc ggaagcccat ttagctgcag
    18361 gagactttat ttggggcttt gaaaagggaa aagactttat tcctttctta aaggaatata
    18421 acggtattat ggtgcgttta gacggatctg acggaatgat caatccttta gagatatttg
    18481 ccacacggac gtatgatgaa gcatccagtt tatatgacga tggatcagtc aaagatttga
    18541 agatcaatga agcagcgtcc tatcaaacac acttagacaa agtggtctat caggtacagt
    18601 tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt taagacctac ttaaatcgtt
    18661 tttatgaaga gtatggaaca gtaccacgcg gctttacttc ctcaaattcg cgttcaaata
    18721 cggagacgca agtcacaggt aaagatccag aagcttatcc gacgttcaaa gaatttttag
    18781 actttttagg acaactggaa ttaccaggtg cttcccaaga gaaaaagaat cgaaaagaag
    18841 aaatggaatc catcgtagaa tccttatgtg agacgtatgg catgattttt gatgggcata
    18901 gtacgattcg acaccttgac caacaacagt tggtcttttt tgacattgac tctattacag
    18961 gcttagatcg aggggtacaa caatgtcaaa tgtacatggc tatgacgttg atttggaatc
    19021 aggcattgat tcaaggaaga agacaacgac
//
LOCUS       DAZKAO010000048        18888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000048 DAZKAO010000000
VERSION     DAZKAO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.048"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8H8_001385"
     CDS             <1..435
                     /locus_tag="ACG8H8_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP8986467.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8H8_001386"
     tRNA            complement(759..842)
                     /locus_tag="ACG8H8_001386"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8H8_001387"
     CDS             1370..1870
                     /locus_tag="ACG8H8_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8986468.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8H8_001388"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8H8_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP8986469.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8H8_001389"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8H8_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8986470.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8H8_001390"
     CDS             3665..4156
                     /locus_tag="ACG8H8_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP8986471.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8H8_001391"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8H8_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP8986472.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8H8_001392"
     CDS             6881..7507
                     /locus_tag="ACG8H8_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8986473.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8H8_001393"
     CDS             7530..8234
                     /locus_tag="ACG8H8_001393"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8986474.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8H8_001394"
     CDS             8426..9259
                     /locus_tag="ACG8H8_001394"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP8986475.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="ACG8H8_001395"
     CDS             9456..11384
                     /locus_tag="ACG8H8_001395"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8986476.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="ACG8H8_001396"
     CDS             11522..11977
                     /locus_tag="ACG8H8_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP8986477.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12057..12185
                     /locus_tag="ACG8H8_001397"
     CDS             12057..12185
                     /locus_tag="ACG8H8_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986478.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12447..12716
                     /locus_tag="ACG8H8_001398"
     CDS             12447..12716
                     /locus_tag="ACG8H8_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986479.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /gene="rpsT"
                     /locus_tag="ACG8H8_001399"
     CDS             13116..13370
                     /gene="rpsT"
                     /locus_tag="ACG8H8_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP8986480.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACG8H8_001400"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACG8H8_001400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP8986481.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="ACG8H8_001401"
     CDS             complement(14543..16816)
                     /locus_tag="ACG8H8_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP8986482.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="ACG8H8_001402"
     CDS             complement(16809..17348)
                     /locus_tag="ACG8H8_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP8986483.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="ACG8H8_001403"
     CDS             complement(17484..17702)
                     /locus_tag="ACG8H8_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP8986484.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="ACG8H8_001404"
     CDS             complement(17699..17944)
                     /locus_tag="ACG8H8_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8986485.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="ACG8H8_001405"
     CDS             complement(18060..18788)
                     /locus_tag="ACG8H8_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP8986486.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5799 a         3655 c         3318 g         6116 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaaca aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gatttttg
//
LOCUS       DAZKAO010000049        18582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000049 DAZKAO010000000
VERSION     DAZKAO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.049"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..237
                     /locus_tag="ACG8H8_001406"
     CDS             154..237
                     /locus_tag="ACG8H8_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP8986487.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..1099
                     /gene="erm(B)"
                     /locus_tag="ACG8H8_001407"
     CDS             362..1099
                     /gene="erm(B)"
                     /locus_tag="ACG8H8_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP8986488.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1362..2225)
                     /locus_tag="ACG8H8_001408"
     CDS             complement(1362..2225)
                     /locus_tag="ACG8H8_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP8986489.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2227..2499)
                     /locus_tag="ACG8H8_001409"
     CDS             complement(2227..2499)
                     /locus_tag="ACG8H8_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP8986490.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2516..2725)
                     /locus_tag="ACG8H8_001410"
     CDS             complement(2516..2725)
                     /locus_tag="ACG8H8_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP8986491.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2824..3720)
                     /locus_tag="ACG8H8_001411"
     CDS             complement(2824..3720)
                     /locus_tag="ACG8H8_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8986492.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(3890..4383)
                     /locus_tag="ACG8H8_001412"
                     /pseudo
     CDS             complement(3890..4383)
                     /locus_tag="ACG8H8_001412"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4459..4590)
                     /locus_tag="ACG8H8_001413"
                     /pseudo
     CDS             complement(4459..4590)
                     /locus_tag="ACG8H8_001413"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(4866..5660)
                     /locus_tag="ACG8H8_001414"
                     /note="aph(3')-IIIa"
     CDS             complement(4866..5660)
                     /locus_tag="ACG8H8_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP8986493.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="ACG8H8_001415"
     CDS             complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="ACG8H8_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP8986494.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(6292..7200)
                     /locus_tag="ACG8H8_001416"
     CDS             complement(6292..7200)
                     /locus_tag="ACG8H8_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP8986495.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(7233..7967)
                     /locus_tag="ACG8H8_001417"
     CDS             complement(7233..7967)
                     /locus_tag="ACG8H8_001417"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8986496.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(7948..8817)
                     /locus_tag="ACG8H8_001418"
     CDS             complement(7948..8817)
                     /locus_tag="ACG8H8_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP8986497.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(8832..9056)
                     /locus_tag="ACG8H8_001419"
     CDS             complement(8832..9056)
                     /locus_tag="ACG8H8_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986498.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(9199..10743)
                     /locus_tag="ACG8H8_001420"
     CDS             complement(9199..10743)
                     /locus_tag="ACG8H8_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8986499.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(10763..11179)
                     /locus_tag="ACG8H8_001421"
     CDS             complement(10763..11179)
                     /locus_tag="ACG8H8_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFP8986500.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(11180..11677)
                     /locus_tag="ACG8H8_001422"
     CDS             complement(11180..11677)
                     /locus_tag="ACG8H8_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFP8986501.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(11988..12665)
                     /locus_tag="ACG8H8_001423"
                     /pseudo
     CDS             complement(11988..12665)
                     /locus_tag="ACG8H8_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(12665..13282)
                     /locus_tag="ACG8H8_001424"
     CDS             complement(12665..13282)
                     /locus_tag="ACG8H8_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8986502.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(13296..13466)
                     /locus_tag="ACG8H8_001425"
     CDS             complement(13296..13466)
                     /locus_tag="ACG8H8_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986503.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(13814..15304)
                     /locus_tag="ACG8H8_001426"
     CDS             complement(13814..15304)
                     /locus_tag="ACG8H8_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP8986504.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(15347..15646)
                     /locus_tag="ACG8H8_001427"
     CDS             complement(15347..15646)
                     /locus_tag="ACG8H8_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986505.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(16012..16296)
                     /locus_tag="ACG8H8_001428"
     CDS             complement(16012..16296)
                     /locus_tag="ACG8H8_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986506.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(16815..17300)
                     /gene="vanZ-A"
                     /locus_tag="ACG8H8_001429"
     CDS             complement(16815..17300)
                     /gene="vanZ-A"
                     /locus_tag="ACG8H8_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP8986507.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(17453..18364)
                     /locus_tag="ACG8H8_001430"
     CDS             complement(17453..18364)
                     /locus_tag="ACG8H8_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP8986508.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5332 a         3746 c         2839 g         6665 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      901 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      961 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1021 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1081 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1141 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1201 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1261 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1321 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1381 ggtgttttgg gtattggtgg tgtaggtagc tgcaagcttt ccattttttg ttgaattgct
     1441 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1501 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1561 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1621 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1681 acaacaatat catgcgcttg ttttggtgtt gccctagctg tcattggatc atctgcatac
     1741 atggtttcat atcgttcaat cgttcctaag tatgattcaa ttttcggtac tgccatgacg
     1801 tatatttttg tttcataacc tttagattga agcattgtgg ctgtttgaat gggaacgtct
     1861 gttgttcgtc ctgttccttc aatcactaaa ttatagcctt gatcgcttaa acggctaata
     1921 agtgcttctg tcatgcgatt cgaataaggg gtaacgtgtt ttactacgtc tttttcatag
     1981 agtttcacta attcgtcaaa atttgggtgc tgttgtttaa aggtatcatt atcaatgaca
     2041 ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt ccctgaccct
     2101 ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctagtctatt tttagttagt
     2161 cgttcaacat tcttttctaa acgatcttca aattgctttt cggtgaagtc agtaatattc
     2221 gccatgttaa gccactttct ctttattcaa aatttgtttt gaatattgat tcattgaccg
     2281 ccaatactca tgaatagctt gtaattcttc aaatggttgt tgaaataaat caacttcttg
     2341 tgccacttct aattgattta aaagattcac ttctagtaaa cgagtatttt tagtatctag
     2401 ttcatgattc aaaacataat ttaatacccg attataaaca cttgccgata actcattaat
     2461 gatttcaatt tcaaatgttt tttcatacgt aactgccatt tcattttcac tccttttaaa
     2521 gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata acttctttta
     2581 cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtcg gcacgaacac
     2641 gaaccgtctt atcccccatt atatcttttt ttgcgctgat tggcgtatca tttcgttttg
     2701 ctttctgtgc gcctaaattt cccacgatcg ctcacttctt tctatttctt cttactattt
     2761 attttatcat cacctatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg
     2821 attctattct ttttcatttt ctaattgaat gattcgctca atcatttcaa aaaatacgtt
     2881 ctcatacata gacaaaactt ttttgtcata gcctttgtgt tctgtaatgc catttttaga
     2941 ccaagtactt actttattac ttcgcttgat aatatttcgg aaaaccaaat tatcctcttt
     3001 atgttctttg tacagttctt ccaggtttga ttttatcgtt gcgctgtccg tatcaactaa
     3061 ataaggaaca aaaccaatca tatctagtcc agggttaaac tgttcttgca aatcaatcaa
     3121 ataggaaata tagttttgaa tgttgtttgt actttcttct tctgcttgta aagggatcat
     3181 aacgtaatca ctcgccacga ttgcattatt tgtataaacg cttggcgttg gtacagtatc
     3241 aataataata agatcatagt cactttttaa aggtgctaaa agagtagcaa gcaatctact
     3301 ttcattttca aacgtccatg agcgagttaa ttttggcagt aacatcaaat caaacgtgcc
     3361 agggatcaag tctaaattat cagtcaaatg aataatagaa gaagccaagt ttccattttt
     3421 caagccttca taaaaattga cacgtggcaa ttctacctca aaagtttttg ctaagtcttt
     3481 tgtcaatgtt gcctgtaagt ccttatcgat cattaaaact tttaaattca atttgtctgt
     3541 caagtaagca aacatagtcg ataatttgga ctttccaaca ccacctttaa agtaattatt
     3601 caagataata attgcttcat ttttattgtt taaaatacgt cttaattctt caagtatttt
     3661 tagtttttcc ttctccataa ctacgcccca ttcttttttt gtatagtaat attgtatcat
     3721 taagaatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg ataagtgatt
     3781 tgtgattaat atataaaagc cctctttaaa gggcttttat atttattttg agaaagatat
     3841 aaaatcaata tatccctttt ccccaatttt tacaacggca ttgtagggca tatagttttt
     3901 tatatgaaag catttgtttt aatatttgtt ccaaagagcc atcatggctt tgacagagct
     3961 cttcttctat gcaaatgctt ttaatccgat ccacacgaaa attcctatat tcatttctta
     4021 gcctgcaaaa ccgattaagt accagttctg ttcataaaac cccagtgata taggctctat
     4081 cattctgctt tccggttctt gccctcctga tgcaggatat tgaattgata ttaccctttt
     4141 attgcagatt gcagtctgaa ttgctgcaat aacgttatcc gccaatgtat ttttctgtcc
     4201 agatgtgctg tacacgttaa tactgttctc caactcctgt gcatagtttt tttcaccatg
     4261 cttcaaaata gatttgattt tatacatagc tgaatcaaaa tcctttataa atgattcatc
     4321 tccatggcaa tttaaaaatt tccctgctgt aatcaatgca cccacttccg ccgctgtaaa
     4381 cattaccggc ggaagtagga agccctctac aagaaaatat ccttttccgg cctcagatcc
     4441 aattggtatt ccggcttctt caagtgtccg gatatctctg taaactgtcc ttaagcttat
     4501 attaaatcgc tgtgcaattt ctttggcagg aattattttt tttgattgca gctgaataag
     4561 aatagaggtt actctgttaa tcctgttcaa attttattcc tcctctgtga cttccataaa
     4621 aagtatatcc tatgcgttct taagctagct gctgttagtg cttctcattg caccctggtt
     4681 ataaaagcca tactcacaaa cctcttcacc aaaactcttt acatctctaa tatagaactc
     4741 tatctagcga acttttagaa aagatataaa acatcagagt atggacagtt gcggatgtac
     4801 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
     4861 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4921 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4981 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     5041 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5101 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5161 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5221 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5281 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5341 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5401 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5461 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5521 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5581 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5641 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5701 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5761 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5821 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
     5881 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
     5941 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
     6001 tataagcgta ccggttccaa ttttttcgca gtttaacttt tccgacgcat ttatcgtctt
     6061 ggtagtaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct
     6121 catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt
     6181 ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg
     6241 gttcgctggg tttatcgata tctttcaggt gccctgcttt catttctgta atcactgttc
     6301 ccgcctctct tctatatcag cggcatatgt gctatccagg cagccggttt taccagtgta
     6361 ttttttatac atgtccctgg tatattttgt tatattccta tcatactccg gataggcata
     6421 atgaagcctt tccgccacct caccggatac cgccctgaac aattgatggc atagaaataa
     6481 tgcttcccat atgttttcat aggaatccat ccggtaggtg gacaaaagtt tctcccacaa
     6541 atcctcggat atataccttt caataaactt atagttcttg cctacactta atttaaagcc
     6601 tgtttcgatg ccgaccttcc atgatatcat tctcagcagc tcatggcgaa caatctgatt
     6661 aaaatgatca atagcaaata aaatttcctt acggcacaat cctttaataa cataaggtgt
     6721 tacattccaa aattcattgc agcaatcatc atactccctt gcgcttggct ttcttacatg
     6781 ataatctata tcagtcggaa ctatgtccct tttaattcta caatctttat caattagaac
     6841 ctttattaat ttatcgccct ttaggtaatt atctaactct tccaagggca ataaggtaag
     6901 atcaatttta ttgtaatcat caaatagcat aagataggaa aatccctttt cttcaggtgg
     6961 gaataattcc atatcctccg gcttttgcat cattattata ttcccaaatt gattaagcca
     7021 gtcatcatta gatataaacg gttctatatc acttacaaaa tatgtaatat cataatcctg
     7081 aaattcatct ttaggtatat taatatttgc gcgtgacccc tcaagggtca caattcgaat
     7141 acgttcatcc tgttctgcta aagaaagtac taaatccatc atttcttttt ctgatctcat
     7201 ttacatactc ctcgtttatt ttttctatat tattacatct tcttttttgc cgatacaatc
     7261 agcatcattg ggcgtcgcat ttcatccgcc atccccggaa tatccatcat gttctctggc
     7321 ggctgtggct ccacaatctg atttattata aaactatttg aaagcagtgt atttagatat
     7381 gtggtcagtg ttctatgata ttttgtaacc ttttcttcca aaaacatagc tgtccgtttg
     7441 ccctcataat aataattgtc caccgggaaa tgcagtattt ctcctttttc gttataatac
     7501 cagtcttgtg ttccatgagc agtaaaaaca ggatgttcaa ctgtaaaaac taaattgcca
     7561 ccagccttca gcatcctata tatctttttt attaaattct catagtctgc tacataatga
     7621 aacgcaagcg aacttagtat tacatcaaag ctctcctctg ggaaatccac atcttctatg
     7681 gcacagcatt catattcaat ctgtggaaaa tgggtttttc cttttgctac ttcgagcatt
     7741 ttatgagaaa tatcaacacc tactacagag gaagcaccgt tttccatcgc atatatacag
     7801 tgccatccat agccgcatcc taaatcaagc acacgcttac ccttaaaatc aggtagcatc
     7861 tttttcaaag tctcccattc tcccgcacca gccagtcctt tctgcgagcg actcatttga
     7921 ctgtattttt gaaaaaatat attatcatca tatttgtttt ctttcatctg aactctcctc
     7981 gtttaaaaag ttatttatct ccgatacaat ttcattcact tctttataaa gtttctcggt
     8041 catgtcgtag cattcaaaaa gagaaagacc gagtacttca aaaatatgat taactctttc
     8101 ggcatatttt tcaggtttat attcaaaagt ttcaagcagt tttatagctt tcttttcgtt
     8161 aatgcaataa gcattattac atgcaaatag tacttgattt aagcatgaaa taatacgaaa
     8221 aacatggcct gcaatataat atttatcctc tgcccccgca tttgctttta caaacattaa
     8281 agagaattca gcttcaaata taaaaaagtt catcaagctt ttctttagag cagtagggta
     8341 aatttctgcc tgctttttta attcgcataa gctttcgttc ttagcatata gtattttact
     8401 aatcgctaat tctccacgat acatagcact tatataacca tggggatgcc cggtctgata
     8461 attggcagta acaattcctt gctccgtatc tttgattatt tgttccaccc gttttatatc
     8521 acgtaatatc aagtcaacat gacacccgtt tataactaac catccaccgc cattaaccca
     8581 atcaccccac gctccgggag gtacaacaag gttgtttcta ttctcatcat ccaactctgt
     8641 agcaatttga ttaatcgctg tcaggtcaaa tgagtctgaa ttgtaataga ttccgatatc
     8701 tatatcagaa ttttctgtat gggtgcctct tgcacgtgag ccccctaata cgatgccttc
     8761 tataaaaggc aaagatgata atttctctgt tactatttga ataacattac ctaccatata
     8821 aacatcctcc tttattcgtt agatattttt atttcttctc catctggtaa aataaatgcc
     8881 tgcacaaact tgacacctag cacatcagca atggccttca actcatccaa ggttactgtt
     8941 tctcgttgta attttttatt gaaattctgt ggagtctggc caatacgtct agcaagttcg
     9001 gaaacactta tattcatttg ctcacacagt tctttaatca tttctgccgt agtcatacag
     9061 cacctccaag tttacgctaa gaatattata aaccatttgg ttgataaaca caatagttat
     9121 taaaatatta ttataatcag caagcaaaag aaaacccaca actctactgt agagtcatgg
     9181 gctatttact tctggttttc atatttccat ttcgatgcca gatttgaagg ctatgacgaa
     9241 gtggccttca tagactgtaa cgctctggat tatcttcctt acaagcttat catcatattc
     9301 taaggtgcgg aatttgttct tgcggataaa ttcaatcagc tcattgattc gctcgttctc
     9361 gccactgagg gaggcatctt ctactaaaag gatctgtcgc ttgtctctca gctcatcaat
     9421 ctcatctgct aagtggtcat agtcttgacc cttgttcgcc agcttgatta gttctttttg
     9481 tttttcctct agtgagttgt taatctctga aatttggtat tctgtggttt cgccaatcac
     9541 agcatgaata ttctcttcca gtgtctttat catattgttc ccacctgcaa gtagcttatt
     9601 aatcgcagtc attactgcac cataaagttc atcttctttt acggttcggt tcttacaaac
     9661 ttcaggacct tgctcgattc tagtcacgca tcgccagaca aattcttttc ttccgtggat
     9721 attccaatag acccgtctat aaatatcgcc acaatctcca cagaaggtga tggcacttaa
     9781 agcgtacttg ctactgtaaa ttcttttgtt cttgccttct cctgtgtaga tattgcttct
     9841 ccgatgaatt tcttcttgcg cctgcaaaaa taactcttta ggaataatcg cttcatggct
     9901 attttcaaca taatattggg gaacatgccc ttcatttttc actcgtttct tggtcagaaa
     9961 atctactgtg acagtctttt gcagaagggc atctccgatg tatttttcgt tttgaagtat
    10021 cttcttaact gattctggtc gccatcttgg ttttcccgct gctgttaaaa taccatcctt
    10081 ttcaagggct cgaccaatgc ccactagact ctgaccctca aggtattctc ggtagatgcg
    10141 tttgataatc tcagcttctt cggggacaat agttaaattt ccatcttcat ctttcgtgta
    10201 gcccataaat cgcttatggt tgacctgcac ctttccttgt tggtatcggt actgtagccc
    10261 aagcttaacg ttttgtgaaa ggctctggct ttcctgctgt gccaaagatg ccataatagt
    10321 cagcaaaacc tcacctttgg catccgtggt gttgatgttc tctttctcaa aataaactga
    10381 tatgttctta tccttgagct gtctaatgta ttgaaggcag tctagggtgt tacgcgcaaa
    10441 tcggctgatg gattttgtaa taaccatgtc gatgttaccg tccatacact ctgcaatcat
    10501 gcgattaaat tcgtcacgct ttttagtgtt cgtaccagag ataccatcat ctgcaaagat
    10561 gccagcaaac tcccattcat tatttttctt tataaactct gtatagtgtg agacctgaac
    10621 ctcatagcta gaattttgtt cttctgtttc tgtagaaaca cggcaatagg cagcaacacg
    10681 aagtttcttt atcttttctt ttgcggctgt acttcctact cttttacgag caggaataac
    10741 cattatattt ttctctgtca ctttattcct cgctttctat cagactgtat aagtattctg
    10801 ctcgttcaaa aggatccact ggcatcttat tatctgcctt tttcattttg aatcgttctt
    10861 tgggaggggg agaggtgaaa gcggcaagct ctaccactcg tcctaaatcc tttgcacgtt
    10921 tatctctaac ttcttcagct ttatcaaata tctctttatc aataattgct ggatacgtat
    10981 catttccaag gtagttgacg tttttcaaaa tgcgccccat cacagaatgt gtcttttcaa
    11041 tacctgcctg ttcgccagcc actgtaaggg atagtcctga tatgtatttc tcaaagaata
    11101 cctttacttg acctgctgcc ttttcatcga cagtaaccac tccatcttga attttgtatc
    11161 catatggaat atatgccatt tatctcacca ccttttcttt cagggaaaga ccgcatttca
    11221 aattgaatgt cagctcatcc ctggaattta caatgatgtt cactacaaat tcttcaaata
    11281 gttcttccgt gtagtcacca ttaaaattat ctgctgacac ataattaatg aggtccttta
    11341 tctcgtttgc tcgcaaaacc ccactcgcag agttcgttac caggtttgtt ttttcggtag
    11401 taagattttt aatctcacta tctaaaacat ttcgttcctg attaaaaaga gcaggttcta
    11461 ggaaaccttt ggacatcagt gcaataaggg tattgcgttc ttccaatagt tgctccatta
    11521 gcttatcaat agcgtccatt ctttcacagt cgctttcttc atcgatttgg ctaactgact
    11581 tgaaaagtgg ttctaggatt agcttatgac tgaaagcaag cttattcatc atggttgtca
    11641 atgtggcttt gatttcccca tctcgcaaaa acaacatgga gcaactttct ttatcttcga
    11701 tactatctca acatgtgtcg tggataaggt aagtatgtaa aatggtatgt ggaacatagt
    11761 gatttgctgt aaatgatttg ctaactactt tagttcttta tacaaagtat aaatcggata
    11821 ttttattatt ttttctttta tatcttctga gattaggtca aagtatttgt ccgctaaatc
    11881 agaaaggggt tcttttttca tcacctaatg ggtgatgaag cagaatactt taaagactat
    11941 taaatcaatg ttaggaaaga ctattagtaa aactatgaat tattcaggaa aaaaggttct
    12001 tttttaaagt tttctattgc ttcctggcgt tgataaatga gatataaagt cggtgtttgt
    12061 acacgtccta atgaaaatgt accttgtacg cctttttgtt gtaaattcaa ggtatataaa
    12121 gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc ttcttgataa
    12181 aagggatagt aattcattcc tggttgcaaa ttttgaaaac cgcttcggat cacatctttt
    12241 tctaagctat tgatccataa tcttttatac gttttatctt tagaaaaggc atttgcttta
    12301 tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc gacaataatt
    12361 gtatttgcct ttttgagaag ttcagcaaca attttaaact gctttccctt atcttttgca
    12421 acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca attttgccac
    12481 ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc aaaggtgata
    12541 aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt tttggtgctt
    12601 tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa aataaccgta
    12661 ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg ttatatcttg
    12721 ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact tctttatcgg
    12781 ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt tctttaaatt
    12841 tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct tgtgcctgac
    12901 gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca atgacaaggc
    12961 tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt aaatttaaga
    13021 cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat tctttattat
    13081 ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga atatagttaa
    13141 gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct gaaaagacct
    13201 tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg ctactgacac
    13261 gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg ttgcaataac
    13321 caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt cttttcgtaa
    13381 gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc gtcattttct
    13441 aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac ttaaatgacc
    13501 tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta tactctaatg
    13561 acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac tgcccctcga
    13621 cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt tttcgggcta
    13681 ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt ctgctaatca
    13741 ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta atggcatagt
    13801 gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg tccgttttag
    13861 ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag tcaaaagaat
    13921 ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg atcgatagca
    13981 acaatgattt aacactagca agttgaatgc caccatttct tcctggttta atcttaaaga
    14041 aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt tcaggaatgc
    14101 ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat acatcgcttt
    14161 tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca cgttgacgtt
    14221 cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct ttactggtta
    14281 aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta gccccttgat
    14341 agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct aagggttgat
    14401 ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa tagcctgaac
    14461 taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa cccttttctc
    14521 ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact tgttttttgc
    14581 cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta tctgtttgtt
    14641 tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca aaaaattcta
    14701 cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga tcaactggca
    14761 aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct ttgacagatt
    14821 taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat gcttgataac
    14881 ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg gttgttaaaa
    14941 tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata aagaaggtat
    15001 tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt tcgtctgcat
    15061 acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga ttttcttgaa
    15121 tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat tttgagcgat
    15181 acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga attttactat
    15241 ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca aaagggatat
    15301 tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa ttaacttggt
    15361 ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt tttcctttat
    15421 aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt aacttatata
    15481 tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag tagatatttc
    15541 tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat tttcttatct
    15601 ccaataaact attttttata taagttttac attcatcatg attcatacaa actccacctt
    15661 ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt aacaattctt
    15721 aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg atagtacggt
    15781 tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag agttcatctg
    15841 actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata aaatgtcgtc
    15901 aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg ttgtttcgtt
    15961 atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta ttcacacgaa
    16021 gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg attctgtttc
    16081 tggttctggt tctgttggct catttgggat taaatccact actagcgttg agttagttaa
    16141 ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt ctattcgtga
    16201 ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt tggtacctct
    16261 catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc tttttgtgta
    16321 ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca agtgattaat
    16381 cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt tcaaaagttt
    16441 ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg catgtttaat
    16501 tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag catggctgct
    16561 agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta ttaaagagac
    16621 caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt gttaggtaag
    16681 ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt ttccccacat
    16741 cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag atcagattgc
    16801 ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac gagcaataat
    16861 acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg attcatatgc
    16921 ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt tgtaattaca
    16981 tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa agtaagactt
    17041 aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt gacattcaaa
    17101 agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa attcccagta
    17161 gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga taaaatattg
    17221 tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc tagcaatcct
    17281 ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt tgtatcatta
    17341 gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt accccaaaag
    17401 tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat ccttacctcc
    17461 ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct atattgtttc
    17521 ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta gtaacaggat
    17581 aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt tcttctttta
    17641 ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca ctatgtggta
    17701 aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct gttttgtcct
    17761 ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac tttccttcag
    17821 gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct gaattatgct
    17881 cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc acactttgct
    17941 catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc tttacagcat
    18001 cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata ttactatcaa
    18061 gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata tctgacttca
    18121 cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct tgataaacct
    18181 gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt tctaaatgtt
    18241 ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac agtgcttcat
    18301 taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa aacaacttct
    18361 tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc tagaatcttc
    18421 cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa
    18481 ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc ataagtgata
    18541 cccatgctga atttaacgag gctgaactgc ctgttgacaa tg
//
LOCUS       DAZKAO010000050        18431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000050 DAZKAO010000000
VERSION     DAZKAO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.050"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..1769
                     /locus_tag="ACG8H8_001431"
     CDS             108..1769
                     /locus_tag="ACG8H8_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8986509.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1839..2186)
                     /locus_tag="ACG8H8_001432"
     CDS             complement(1839..2186)
                     /locus_tag="ACG8H8_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP8986510.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2270..3211
                     /gene="opp3b"
                     /locus_tag="ACG8H8_001433"
     CDS             2270..3211
                     /gene="opp3b"
                     /locus_tag="ACG8H8_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP8986511.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3212..4255
                     /locus_tag="ACG8H8_001434"
     CDS             3212..4255
                     /locus_tag="ACG8H8_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986512.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4261..5331
                     /locus_tag="ACG8H8_001435"
     CDS             4261..5331
                     /locus_tag="ACG8H8_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986513.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5321..6274
                     /locus_tag="ACG8H8_001436"
     CDS             5321..6274
                     /locus_tag="ACG8H8_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986514.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6286..7296
                     /locus_tag="ACG8H8_001437"
     CDS             6286..7296
                     /locus_tag="ACG8H8_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP8986515.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7581..8402)
                     /locus_tag="ACG8H8_001438"
     CDS             complement(7581..8402)
                     /locus_tag="ACG8H8_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP8986516.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8499..8777)
                     /locus_tag="ACG8H8_001439"
     CDS             complement(8499..8777)
                     /locus_tag="ACG8H8_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986517.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9045..11054
                     /gene="metG"
                     /locus_tag="ACG8H8_001440"
     CDS             9045..11054
                     /gene="metG"
                     /locus_tag="ACG8H8_001440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP8986518.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11533..12120)
                     /locus_tag="ACG8H8_001441"
     CDS             complement(11533..12120)
                     /locus_tag="ACG8H8_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986519.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12295..13617
                     /locus_tag="ACG8H8_001442"
     CDS             12295..13617
                     /locus_tag="ACG8H8_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8986520.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13772..14548
                     /locus_tag="ACG8H8_001443"
     CDS             13772..14548
                     /locus_tag="ACG8H8_001443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP8986521.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14541..15110
                     /gene="rnmV"
                     /locus_tag="ACG8H8_001444"
     CDS             14541..15110
                     /gene="rnmV"
                     /locus_tag="ACG8H8_001444"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP8986522.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15103..15987
                     /gene="rsmA"
                     /locus_tag="ACG8H8_001445"
     CDS             15103..15987
                     /gene="rsmA"
                     /locus_tag="ACG8H8_001445"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP8986523.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16438..17067
                     /locus_tag="ACG8H8_001446"
     CDS             16438..17067
                     /locus_tag="ACG8H8_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986524.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17191..18300)
                     /locus_tag="ACG8H8_001447"
     CDS             complement(17191..18300)
                     /locus_tag="ACG8H8_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986525.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6020 a         3320 c         3758 g         5333 t
ORIGIN      
        1 aaaaagacaa aaaaaagtgt tgatttttta ataattattc attaaactct taaacaactt
       61 cagaaaatat taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat
      121 ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt
      181 cagccacaag cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact
      241 tagtcgtgcc tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt
      301 ttacagcatt gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac
      361 agccggcagg agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca
      421 aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt
      481 atggctggca acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc
      541 cagttgaaaa tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca
      601 aagcagttgg tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat
      661 atctgcttgc tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg
      721 atgcctatgc atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt
      781 ttgatggacc aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca
      841 aagatacagt aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat
      901 tgaacttatt caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc
      961 aaaacgcaaa cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat
     1021 tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt
     1081 attctatcga tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta
     1141 caggattgat cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag
     1201 aagctggaaa attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga
     1261 aaaaagaatt gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat
     1321 cgaaaaaagt aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag
     1381 tcaaaccaac acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg
     1441 acgcagtact aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt
     1501 ttgtcacagg taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag
     1561 ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa
     1621 aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag
     1681 gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg
     1741 attacaaatg gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga
     1801 ggctgcggca aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc
     1861 atgaaaatag cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata
     1921 ccgtagctgg cagacgagag cgaggaaaat tctggttttt tctttcggtt ccttgttcgt
     1981 gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa
     2041 ccgccgataa tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt
     2101 gtaaaggaat ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact
     2161 gcgtagggga tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa
     2221 taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta
     2281 tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac
     2341 aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt
     2401 gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt
     2461 acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt
     2521 taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg
     2581 gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat
     2641 gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat
     2701 tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt
     2761 gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat
     2821 gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag
     2881 tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca
     2941 tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt
     3001 gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt
     3061 cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc
     3121 atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag
     3181 aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac
     3241 agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg
     3301 tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa
     3361 aaacaaagct gccgttattt caatggtttt actattggtc atcattttga tctctgtgat
     3421 cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc
     3481 tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga
     3541 acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac
     3601 agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct
     3661 gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc
     3721 tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg
     3781 aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc
     3841 aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca
     3901 aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag
     3961 aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat
     4021 gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg
     4081 tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct
     4141 gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca tgattggctt
     4201 taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg
     4261 atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag
     4321 gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt
     4381 ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc
     4441 aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca
     4501 gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg
     4561 acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat
     4621 aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga
     4681 atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt
     4741 caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa
     4801 cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt
     4861 cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat
     4921 gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa
     4981 gaaattttct acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca
     5041 ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac
     5101 ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa
     5161 caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct
     5221 ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa
     5281 gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga
     5341 agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa
     5401 tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag
     5461 cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat
     5521 attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg
     5581 gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt
     5641 cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg
     5701 tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg
     5761 ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc
     5821 agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca
     5881 agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt
     5941 tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa
     6001 cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta
     6061 tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag
     6121 aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc
     6181 accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa
     6241 acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt
     6301 aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt
     6361 gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga
     6421 gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa
     6481 gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat
     6541 ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat
     6601 acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa
     6661 caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct
     6721 acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca
     6781 gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc
     6841 caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg
     6901 caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg
     6961 ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa
     7021 caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat
     7081 gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac
     7141 gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt
     7201 cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta
     7261 gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc
     7321 gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt
     7381 ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac
     7441 gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct
     7501 gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc
     7561 tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa
     7621 ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc
     7681 ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt
     7741 cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc
     7801 attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa
     7861 gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta
     7921 gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca
     7981 tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag
     8041 cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa
     8101 ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc
     8161 ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg
     8221 atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca
     8281 gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct
     8341 tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc
     8401 atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa
     8461 aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga
     8521 agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca
     8581 cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa
     8641 gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata
     8701 taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa
     8761 agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac
     8821 cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc
     8881 cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt
     8941 tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag
     9001 caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa
     9061 ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt
     9121 atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt
     9181 tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag
     9241 gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa
     9301 cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg
     9361 ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag
     9421 gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca
     9481 aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg
     9541 taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact
     9601 atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact
     9661 ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac
     9721 cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata
     9781 ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag
     9841 atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc
     9901 tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga
     9961 aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac
    10021 gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg
    10081 gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag
    10141 ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg
    10201 actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg
    10261 tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct
    10321 gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa
    10381 aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc
    10441 gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc
    10501 aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt
    10561 caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag
    10621 aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca
    10681 aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg
    10741 tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg
    10801 cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt
    10861 caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg
    10921 tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg
    10981 cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct
    11041 cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc
    11101 cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat
    11161 acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt
    11221 tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag cataatgata
    11281 aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc cttacattca
    11341 taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt agcgttgatg
    11401 tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat atgtgtgatt
    11461 gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt tctttatttt
    11521 gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca aacggcttgg
    11581 taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc
    11641 gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca accctaaaag
    11701 caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa gtgccatcac
    11761 tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt tctttgtaaa
    11821 tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag
    11881 cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt tgatcaatgc
    11941 aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg caacagtcat
    12001 taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt ctttacggga
    12061 aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct taaaatccat
    12121 aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa aaagaaatac
    12181 atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt aaattaaaat
    12241 attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa
    12301 ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat gggaaccgtg
    12361 atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca gtctacaagt
    12421 ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg attgacagtg
    12481 gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat tgaagctgcg
    12541 tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta ctttcctgct
    12601 aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt acaattatct
    12661 accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg gttgaatttg
    12721 ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa attaatacca
    12781 attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt ccagttattt
    12841 ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc gttgttagca
    12901 acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg
    12961 ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac agtcgtttat
    13021 ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc cggtaatttg
    13081 aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa gctggtcact
    13141 attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac tgggatccgc
    13201 gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct gacaaagctt
    13261 tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt agcttgtatt
    13321 atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt gatgtggcta
    13381 ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca
    13441 cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct cggtgctatt
    13501 ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg tataggtgtt
    13561 accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat
    13621 atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt gctttttcta
    13681 tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta gtgcttatgt
    13741 gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata cccatttaaa
    13801 tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag aaatgggggt
    13861 aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt tgcaattaag
    13921 ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg ctggcagtta
    13981 tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg ttgtagcatt
    14041 aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc aagataaagt
    14101 atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta ttcatactag
    14161 agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag atatcggtgg
    14221 aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg acttagggat
    14281 gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc
    14341 agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc tggcaccagt
    14401 tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag aaaagattgc
    14461 tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa atgcgcaccg
    14521 attatttggt ctaagcgaac atggataaga tgaagatcca agagattata gtcgttgaag
    14581 gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata gaaacaatag
    14641 ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag actcgtggag
    14701 tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc attatggaag
    14761 ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca aagaaaaagg
    14821 gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg
    14881 tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac cttttagact
    14941 atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt gacgagttgc
    15001 gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt cgtattacgc
    15061 cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga atatcgtgaa
    15121 attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc atttaaaaaa
    15181 agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt agaaactgcg
    15241 caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc attgacggaa
    15301 caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg tttgatccct
    15361 gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca agatatttta
    15421 aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc gttaaaagtc
    15481 gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct tgaatcagat
    15541 ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg tatctctgct
    15601 gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta tatggaagcc
    15661 agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt agattcagcg
    15721 attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga aaaagcattt
    15781 ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa taacttgcag
    15841 cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt ggaaacagct
    15901 ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc cgcattaagc
    15961 aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc
    16021 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg
    16081 tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc
    16141 ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc atgtttttga
    16201 gttagttggt cttattactt gatgcaagct tttgaccact attatgccga acaaggagat
    16261 gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat tcttatactt
    16321 gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac agttattagt
    16381 aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag agaaaaaatg
    16441 agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat gggggtcatt
    16501 atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt ggcgtctcat
    16561 gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt tgcgctaggt
    16621 acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc attgtcacat
    16681 cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa
    16741 aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt gatcggtgtg
    16801 attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg taatatgcct
    16861 gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg gattggtggg
    16921 cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac actcagcaaa
    16981 tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa
    17041 gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac tctcatctag
    17101 taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc tgcaccaaca
    17161 tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt gttctgtttc
    17221 tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt cgcctggttt
    17281 atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt ttgtgtaaag
    17341 catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta cggcttcagg
    17401 tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac cgaagataac
    17461 ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag gaatacgaag
    17521 cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa agaagttcat
    17581 ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat aaacttcttt
    17641 cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt cagccatagt
    17701 cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac gctggtgaag
    17761 tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg ataatggctc
    17821 atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca cacgttggcg
    17881 ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac ctaagatctc
    17941 tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta atttcaagcc
    18001 aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt ggaaaaccat
    18061 ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga tatttagttc
    18121 tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact ttccacaacc
    18181 ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa aatcagttac
    18241 agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca tttctaccat
    18301 aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa atgaaagcgt
    18361 taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca atgtgctagt
    18421 tttaagcttt a
//
LOCUS       DAZKAO010000051        18211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000051 DAZKAO010000000
VERSION     DAZKAO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.051"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      53..219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            367..924
                     /locus_tag="ACG8H8_001448"
                     /pseudo
     CDS             367..924
                     /locus_tag="ACG8H8_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            982..3021
                     /locus_tag="ACG8H8_001449"
     CDS             982..3021
                     /locus_tag="ACG8H8_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP8986526.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3130..4281)
                     /locus_tag="ACG8H8_001450"
     CDS             complement(3130..4281)
                     /locus_tag="ACG8H8_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP8986527.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4435..4965)
                     /locus_tag="ACG8H8_001451"
     CDS             complement(4435..4965)
                     /locus_tag="ACG8H8_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP8986528.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5064..5414)
                     /locus_tag="ACG8H8_001452"
     CDS             complement(5064..5414)
                     /locus_tag="ACG8H8_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP8986529.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5571..7100)
                     /gene="ahpF"
                     /locus_tag="ACG8H8_001453"
     CDS             complement(5571..7100)
                     /gene="ahpF"
                     /locus_tag="ACG8H8_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP8986530.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7110..7673)
                     /gene="ahpC"
                     /locus_tag="ACG8H8_001454"
     CDS             complement(7110..7673)
                     /gene="ahpC"
                     /locus_tag="ACG8H8_001454"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP8986531.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7817..8479)
                     /locus_tag="ACG8H8_001455"
     CDS             complement(7817..8479)
                     /locus_tag="ACG8H8_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8986532.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8663..10309
                     /locus_tag="ACG8H8_001456"
     CDS             8663..10309
                     /locus_tag="ACG8H8_001456"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP8986533.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10300..11382
                     /gene="uxuA"
                     /locus_tag="ACG8H8_001457"
     CDS             10300..11382
                     /gene="uxuA"
                     /locus_tag="ACG8H8_001457"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8986534.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11490..13043)
                     /locus_tag="ACG8H8_001458"
                     /pseudo
     CDS             complement(11490..13043)
                     /locus_tag="ACG8H8_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13191..14332
                     /locus_tag="ACG8H8_001459"
                     /pseudo
     CDS             13191..14332
                     /locus_tag="ACG8H8_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14458..15537)
                     /locus_tag="ACG8H8_001460"
     CDS             complement(14458..15537)
                     /locus_tag="ACG8H8_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8986535.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15640..16080)
                     /locus_tag="ACG8H8_001461"
     CDS             complement(15640..16080)
                     /locus_tag="ACG8H8_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8986536.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16137..16547)
                     /locus_tag="ACG8H8_001462"
     CDS             complement(16137..16547)
                     /locus_tag="ACG8H8_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP8986537.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16727..17161)
                     /locus_tag="ACG8H8_001463"
     CDS             complement(16727..17161)
                     /locus_tag="ACG8H8_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8986538.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17261..18109)
                     /locus_tag="ACG8H8_001464"
     CDS             complement(17261..18109)
                     /locus_tag="ACG8H8_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8986539.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6247 a         3373 c         3287 g         5304 t
ORIGIN      
        1 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
       61 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
      121 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      181 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      241 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      301 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      361 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      421 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      481 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      541 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      601 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      661 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      721 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      781 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      841 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
      901 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
      961 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     1021 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1081 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1141 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1201 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1261 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1321 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1381 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1441 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1501 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1561 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1621 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1681 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1741 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1801 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1861 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     1921 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     1981 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     2041 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2101 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2161 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2221 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2281 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2341 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2401 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2461 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2521 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2581 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2641 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2701 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2761 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2821 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     2881 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     2941 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     3001 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3061 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3121 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3181 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3241 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3301 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3361 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3421 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3481 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3541 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3601 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3661 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3721 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3781 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     3841 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     3901 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     3961 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     4021 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     4081 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     4141 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     4201 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     4261 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     4321 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     4381 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     4441 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     4501 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     4561 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     4621 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     4681 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     4741 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     4801 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     4861 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     4921 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     4981 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     5041 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     5101 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     5161 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     5221 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     5281 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     5341 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     5401 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     5461 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt
     5521 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
     5581 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
     5641 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
     5701 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttct atctagccaa
     5761 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
     5821 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
     5881 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
     5941 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
     6001 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
     6061 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
     6121 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
     6181 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
     6241 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
     6301 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
     6361 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
     6421 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
     6481 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     6541 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     6601 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     6661 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     6721 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     6781 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     6841 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     6901 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     6961 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     7021 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     7081 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     7141 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     7201 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     7261 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     7321 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     7381 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     7441 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     7501 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     7561 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     7621 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa
     7681 cctccaataa taattattta tttataatta ttgtaattta gttggagttg caaagtcaat
     7741 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
     7801 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
     7861 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
     7921 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
     7981 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
     8041 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
     8101 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
     8161 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
     8221 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
     8281 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
     8341 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
     8401 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
     8461 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
     8521 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
     8581 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
     8641 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
     8701 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     8761 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     8821 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     8881 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     8941 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
     9001 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
     9061 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
     9121 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
     9181 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
     9241 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
     9301 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
     9361 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
     9421 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
     9481 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
     9541 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
     9601 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
     9661 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
     9721 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
     9781 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
     9841 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
     9901 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
     9961 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
    10021 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
    10081 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
    10141 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
    10201 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
    10261 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
    10321 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
    10381 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
    10441 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
    10501 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
    10561 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
    10621 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
    10681 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
    10741 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
    10801 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
    10861 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
    10921 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
    10981 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
    11041 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
    11101 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
    11161 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
    11221 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
    11281 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
    11341 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
    11401 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
    11461 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
    11521 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
    11581 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
    11641 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
    11701 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
    11761 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
    11821 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
    11881 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
    11941 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
    12001 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
    12061 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
    12121 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
    12181 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
    12241 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
    12301 tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa
    12361 ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg
    12421 tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca
    12481 tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat
    12541 tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac
    12601 aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca
    12661 ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa
    12721 gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag
    12781 taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc
    12841 agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa
    12901 tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt
    12961 tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt
    13021 ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg
    13081 aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat
    13141 ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt
    13201 caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag
    13261 tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc
    13321 gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag
    13381 gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac
    13441 aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca
    13501 ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact
    13561 tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt
    13621 taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag
    13681 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
    13741 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
    13801 aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag
    13861 atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag
    13921 acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag
    13981 atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca
    14041 aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa
    14101 aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg
    14161 aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct
    14221 accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag
    14281 tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa
    14341 taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata
    14401 ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta
    14461 agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa
    14521 aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct
    14581 aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt
    14641 cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac
    14701 aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat
    14761 aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg
    14821 atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac
    14881 aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat
    14941 atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt
    15001 tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat
    15061 ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa
    15121 aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg
    15181 ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat gaataagcat
    15241 cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat
    15301 agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat
    15361 caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag
    15421 aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg
    15481 ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat
    15541 atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
    15601 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
    15661 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
    15721 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
    15781 accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
    15841 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
    15901 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
    15961 aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt gtctaatgat
    16021 ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
    16081 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
    16141 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
    16201 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
    16261 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
    16321 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
    16381 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
    16441 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg cacttctcca
    16501 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct
    16561 ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg
    16621 attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa
    16681 catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac atatatttct
    16741 caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga agcatccata
    16801 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
    16861 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
    16921 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
    16981 gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa atgacttcat
    17041 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
    17101 acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt cctttatgca
    17161 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagctaa
    17221 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
    17281 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
    17341 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
    17401 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
    17461 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
    17521 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
    17581 ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga gaaacaacga
    17641 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
    17701 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    17761 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    17821 ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg
    17881 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    17941 ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg ctgacacctc
    18001 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    18061 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tattcacttc
    18121 cttaattcat taataatact cttatcttat tataacttac ttttcacctt aagttaactt
    18181 taattaataa aaaaattagg ctagtctaaa t
//
LOCUS       DAZKAO010000052        17835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000052 DAZKAO010000000
VERSION     DAZKAO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.052"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..936
                     /locus_tag="ACG8H8_001465"
     CDS             124..936
                     /locus_tag="ACG8H8_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP8986540.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            933..1733
                     /locus_tag="ACG8H8_001466"
     CDS             933..1733
                     /locus_tag="ACG8H8_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP8986541.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1894..3162
                     /gene="rseP"
                     /locus_tag="ACG8H8_001467"
     CDS             1894..3162
                     /gene="rseP"
                     /locus_tag="ACG8H8_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP8986542.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3230..4939
                     /locus_tag="ACG8H8_001468"
     CDS             3230..4939
                     /locus_tag="ACG8H8_001468"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP8986543.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5151..9503
                     /locus_tag="ACG8H8_001469"
     CDS             5151..9503
                     /locus_tag="ACG8H8_001469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP8986544.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9647..10120
                     /gene="rimP"
                     /locus_tag="ACG8H8_001470"
     CDS             9647..10120
                     /gene="rimP"
                     /locus_tag="ACG8H8_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP8986545.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10144..11319
                     /gene="nusA"
                     /locus_tag="ACG8H8_001471"
     CDS             10144..11319
                     /gene="nusA"
                     /locus_tag="ACG8H8_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP8986546.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11341..11634
                     /locus_tag="ACG8H8_001472"
     CDS             11341..11634
                     /locus_tag="ACG8H8_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP8986547.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11631..11942
                     /locus_tag="ACG8H8_001473"
     CDS             11631..11942
                     /locus_tag="ACG8H8_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP8986548.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11955..14261
                     /gene="infB"
                     /locus_tag="ACG8H8_001474"
     CDS             11955..14261
                     /gene="infB"
                     /locus_tag="ACG8H8_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP8986549.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14288..14635
                     /gene="rbfA"
                     /locus_tag="ACG8H8_001475"
     CDS             14288..14635
                     /gene="rbfA"
                     /locus_tag="ACG8H8_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP8986550.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14743..15247)
                     /locus_tag="ACG8H8_001476"
                     /pseudo
     CDS             complement(14743..15247)
                     /locus_tag="ACG8H8_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15412..17775
                     /locus_tag="ACG8H8_001477"
     CDS             15412..17775
                     /locus_tag="ACG8H8_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFP8986551.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6096 a         3154 c         3920 g         4665 t
ORIGIN      
        1 attaaaaaaa gaaaaaatta ttcagttttt aaataaaaaa acaatgattt cataggaaaa
       61 acagttagat attgctataa tacttaagat agtgggaatc ttttaagtat aggaggaaaa
      121 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
      181 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      241 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      301 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      361 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      421 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      481 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      541 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      601 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      661 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      721 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      781 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      841 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      901 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      961 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
     1021 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
     1081 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1141 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1201 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1261 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1321 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1381 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1441 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1501 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1561 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1621 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1681 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1741 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1801 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1861 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1921 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1981 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     2041 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     2101 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2161 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2221 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2281 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2341 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2401 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2461 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2521 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2581 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2641 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2701 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2761 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2821 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2881 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2941 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     3001 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     3061 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3121 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3181 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3241 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3301 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3361 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3421 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3481 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3541 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3601 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3661 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3721 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3781 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3841 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3901 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3961 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     4021 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     4081 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4141 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4201 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4261 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4321 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4381 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4441 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4501 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4561 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4621 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4681 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4741 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4801 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4861 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4921 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4981 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     5041 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     5101 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5161 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5221 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5281 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5341 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5401 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5461 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5521 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5581 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5641 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5701 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5761 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5821 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5881 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5941 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     6001 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     6061 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6121 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6181 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6241 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6301 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6361 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6421 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6481 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6541 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6601 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6661 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6721 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6781 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6841 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6901 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6961 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     7021 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     7081 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7141 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7201 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7261 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7321 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7381 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7441 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7501 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7561 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7621 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7681 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7741 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7801 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7861 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7921 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7981 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     8041 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     8101 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8161 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8221 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8281 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8341 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8401 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8461 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8521 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8581 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8641 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8701 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8761 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8821 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8881 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8941 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     9001 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     9061 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9121 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9181 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9241 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9301 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9361 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9421 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9481 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9541 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9601 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9661 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9721 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9781 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9841 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9901 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9961 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
    10021 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
    10081 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10141 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10201 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10261 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10321 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10381 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10441 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10501 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10561 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10621 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10681 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10741 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10801 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10861 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10921 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10981 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    11041 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    11101 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11161 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11221 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11281 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11341 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11401 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11461 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11521 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11581 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11641 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11701 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11761 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11821 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11881 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11941 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    12001 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    12061 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12121 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12181 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12241 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12301 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12361 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12421 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12481 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12541 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12601 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12661 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12721 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12781 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12841 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12901 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12961 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    13021 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    13081 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13141 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13201 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13261 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13321 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13381 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13441 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13501 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13561 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13621 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13681 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13741 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13801 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13861 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13921 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13981 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    14041 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    14101 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14161 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14221 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14281 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14341 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14401 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14461 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14521 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14581 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14641 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14701 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14761 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14821 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14881 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14941 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    15001 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    15061 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15121 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15181 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15241 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15301 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15361 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15421 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15481 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15541 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15601 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15661 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15721 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15781 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15841 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15901 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15961 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    16021 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    16081 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16141 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16201 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16261 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16321 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16381 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16441 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16501 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16561 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16621 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16681 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16741 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16801 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16861 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16921 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16981 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    17041 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    17101 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17161 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17221 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17281 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17341 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17401 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17461 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17521 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17581 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17641 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17701 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17761 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17821 atttgtcaaa ttaag
//
LOCUS       DAZKAO010000053        17790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000053 DAZKAO010000000
VERSION     DAZKAO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.053"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..925
                     /locus_tag="ACG8H8_001478"
     CDS             95..925
                     /locus_tag="ACG8H8_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP8986552.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1169..1418)
                     /locus_tag="ACG8H8_001479"
                     /pseudo
     CDS             complement(1169..1418)
                     /locus_tag="ACG8H8_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1609..1803)
                     /locus_tag="ACG8H8_001480"
     CDS             complement(1609..1803)
                     /locus_tag="ACG8H8_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986553.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2308..2493)
                     /locus_tag="ACG8H8_001481"
     CDS             complement(2308..2493)
                     /locus_tag="ACG8H8_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986554.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3343..3684)
                     /locus_tag="ACG8H8_001482"
     CDS             complement(3343..3684)
                     /locus_tag="ACG8H8_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP8986555.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3684..3914)
                     /locus_tag="ACG8H8_001483"
     CDS             complement(3684..3914)
                     /locus_tag="ACG8H8_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8986556.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4201..4539
                     /locus_tag="ACG8H8_001484"
     CDS             4201..4539
                     /locus_tag="ACG8H8_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986557.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4744..5306)
                     /locus_tag="ACG8H8_001485"
                     /pseudo
     CDS             complement(4744..5306)
                     /locus_tag="ACG8H8_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6038..6619)
                     /locus_tag="ACG8H8_001486"
     CDS             complement(6038..6619)
                     /locus_tag="ACG8H8_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8986558.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6802..7428)
                     /locus_tag="ACG8H8_001487"
     CDS             complement(6802..7428)
                     /locus_tag="ACG8H8_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP8986559.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7450..8781)
                     /locus_tag="ACG8H8_001488"
     CDS             complement(7450..8781)
                     /locus_tag="ACG8H8_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP8986560.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8936..9337)
                     /locus_tag="ACG8H8_001489"
     CDS             complement(8936..9337)
                     /locus_tag="ACG8H8_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP8986561.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9423..9629)
                     /locus_tag="ACG8H8_001490"
     CDS             complement(9423..9629)
                     /locus_tag="ACG8H8_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986562.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9724..11226)
                     /locus_tag="ACG8H8_001491"
     CDS             complement(9724..11226)
                     /locus_tag="ACG8H8_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP8986563.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11244..11699)
                     /gene="argR"
                     /locus_tag="ACG8H8_001492"
     CDS             complement(11244..11699)
                     /gene="argR"
                     /locus_tag="ACG8H8_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8986564.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11797..12414)
                     /locus_tag="ACG8H8_001493"
     CDS             complement(11797..12414)
                     /locus_tag="ACG8H8_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8986565.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12635..14008
                     /locus_tag="ACG8H8_001494"
     CDS             12635..14008
                     /locus_tag="ACG8H8_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8986566.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14083..15195
                     /locus_tag="ACG8H8_001495"
     CDS             14083..15195
                     /locus_tag="ACG8H8_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8986567.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15267..16904)
                     /locus_tag="ACG8H8_001496"
     CDS             complement(15267..16904)
                     /locus_tag="ACG8H8_001496"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP8986568.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17072..17680)
                     /locus_tag="ACG8H8_001497"
     CDS             complement(17072..17680)
                     /locus_tag="ACG8H8_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8986569.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5032 a         3633 c         3116 g         6009 t
ORIGIN      
        1 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
       61 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
      121 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
      181 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
      241 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
      301 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
      361 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
      421 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
      481 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
      541 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
      601 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
      661 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
      721 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
      781 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
      841 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
      901 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
      961 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
     1021 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
     1081 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
     1141 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
     1201 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
     1261 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
     1321 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
     1381 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
     1441 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
     1501 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
     1561 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
     1621 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
     1681 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
     1741 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
     1801 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
     1861 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
     1921 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
     1981 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
     2041 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
     2101 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
     2161 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
     2221 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
     2281 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
     2341 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
     2401 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
     2461 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
     2521 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaacc
     2581 ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga
     2641 tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa
     2701 ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca
     2761 ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta
     2821 aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt
     2881 gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt
     2941 cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga
     3001 gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact
     3061 aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata
     3121 cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga
     3181 aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta
     3241 atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt
     3301 atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat
     3361 aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg
     3421 ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc
     3481 ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac
     3541 cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt
     3601 ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg
     3661 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
     3721 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
     3781 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
     3841 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
     3901 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
     3961 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
     4021 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
     4081 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
     4141 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
     4201 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
     4261 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
     4321 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
     4381 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
     4441 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
     4501 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
     4561 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
     4621 actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt
     4681 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
     4741 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
     4801 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
     4861 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
     4921 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
     4981 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
     5041 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
     5101 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
     5161 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
     5221 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
     5281 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
     5341 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
     5401 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
     5461 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
     5521 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
     5581 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac
     5641 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     5701 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
     5761 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
     5821 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
     5881 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
     5941 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
     6001 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
     6061 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
     6121 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
     6181 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
     6241 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
     6301 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
     6361 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
     6421 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
     6481 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
     6541 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
     6601 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
     6661 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
     6721 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
     6781 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
     6841 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
     6901 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
     6961 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
     7021 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
     7081 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
     7141 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
     7201 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
     7261 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
     7321 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
     7381 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
     7441 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
     7501 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
     7561 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
     7621 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
     7681 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
     7741 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
     7801 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
     7861 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
     7921 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
     7981 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
     8041 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
     8101 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
     8161 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
     8221 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
     8281 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
     8341 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
     8401 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
     8461 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
     8521 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
     8581 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
     8641 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
     8701 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
     8761 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
     8821 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
     8881 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
     8941 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
     9001 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
     9061 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
     9121 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
     9181 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
     9241 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
     9301 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
     9361 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
     9421 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
     9481 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
     9541 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
     9601 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
     9661 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
     9721 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
     9781 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
     9841 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
     9901 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
     9961 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
    10021 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
    10081 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
    10141 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
    10201 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
    10261 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
    10321 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
    10381 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
    10441 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
    10501 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
    10561 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
    10621 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
    10681 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
    10741 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
    10801 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
    10861 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
    10921 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
    10981 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
    11041 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
    11101 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
    11161 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
    11221 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
    11281 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
    11341 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
    11401 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
    11461 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
    11521 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
    11581 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
    11641 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
    11701 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
    11761 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
    11821 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
    11881 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
    11941 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
    12001 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
    12061 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
    12121 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
    12181 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
    12241 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
    12301 attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg
    12361 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
    12421 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
    12481 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
    12541 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
    12601 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
    12661 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
    12721 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
    12781 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
    12841 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
    12901 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
    12961 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
    13021 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
    13081 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
    13141 gtttcttgtg cccgttttgt ttctttcttt gattatcggc tggtttgcta ttcccgagat
    13201 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    13261 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    13321 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
    13381 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    13441 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    13501 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    13561 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    13621 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    13681 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    13741 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    13801 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    13861 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    13921 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    13981 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    14041 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    14101 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    14161 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    14221 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    14281 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    14341 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    14401 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    14461 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    14521 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    14581 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    14641 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    14701 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    14761 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    14821 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    14881 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    14941 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    15001 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    15061 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    15121 ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg
    15181 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    15241 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    15301 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    15361 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    15421 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    15481 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    15541 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    15601 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    15661 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    15721 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    15781 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    15841 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    15901 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    15961 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    16021 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    16081 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    16141 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    16201 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    16261 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    16321 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    16381 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    16441 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    16501 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    16561 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    16621 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    16681 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    16741 atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga
    16801 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    16861 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    16921 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    16981 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    17041 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    17101 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    17161 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    17221 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    17281 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    17341 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    17401 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    17461 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    17521 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    17581 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    17641 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    17701 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    17761 aaacatgaca tttaatgaca aaataacgaa
//
LOCUS       DAZKAO010000054        17570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000054 DAZKAO010000000
VERSION     DAZKAO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.054"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1668)
                     /locus_tag="ACG8H8_001498"
     CDS             complement(88..1668)
                     /locus_tag="ACG8H8_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP8986570.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2069..3445)
                     /locus_tag="ACG8H8_001499"
     CDS             complement(2069..3445)
                     /locus_tag="ACG8H8_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP8986571.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3573..4661)
                     /locus_tag="ACG8H8_001500"
     CDS             complement(3573..4661)
                     /locus_tag="ACG8H8_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8986572.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4833..5267
                     /locus_tag="ACG8H8_001501"
     CDS             4833..5267
                     /locus_tag="ACG8H8_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8986573.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5345..5878)
                     /locus_tag="ACG8H8_001502"
     CDS             complement(5345..5878)
                     /locus_tag="ACG8H8_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP8986574.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="ACG8H8_001503"
     CDS             complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="ACG8H8_001503"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP8986575.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7143..7940)
                     /gene="recX"
                     /locus_tag="ACG8H8_001504"
     CDS             complement(7143..7940)
                     /gene="recX"
                     /locus_tag="ACG8H8_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP8986576.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8047..9429
                     /gene="rlmD"
                     /locus_tag="ACG8H8_001505"
     CDS             8047..9429
                     /gene="rlmD"
                     /locus_tag="ACG8H8_001505"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP8986577.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9603..10181)
                     /locus_tag="ACG8H8_001506"
     CDS             complement(9603..10181)
                     /locus_tag="ACG8H8_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP8986578.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10503..10973
                     /locus_tag="ACG8H8_001507"
     CDS             10503..10973
                     /locus_tag="ACG8H8_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP8986579.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11081..12307)
                     /locus_tag="ACG8H8_001508"
     CDS             complement(11081..12307)
                     /locus_tag="ACG8H8_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8986580.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13061..13261)
                     /locus_tag="ACG8H8_001509"
     CDS             complement(13061..13261)
                     /locus_tag="ACG8H8_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8986581.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13395..14003)
                     /locus_tag="ACG8H8_001510"
     CDS             complement(13395..14003)
                     /locus_tag="ACG8H8_001510"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP8986582.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14088..14558)
                     /locus_tag="ACG8H8_001511"
     CDS             complement(14088..14558)
                     /locus_tag="ACG8H8_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP8986583.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14606..15520)
                     /locus_tag="ACG8H8_001512"
     CDS             complement(14606..15520)
                     /locus_tag="ACG8H8_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8986584.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15666..16469
                     /locus_tag="ACG8H8_001513"
     CDS             15666..16469
                     /locus_tag="ACG8H8_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP8986585.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16647..>17570
                     /locus_tag="ACG8H8_001514"
     CDS             16647..>17570
                     /locus_tag="ACG8H8_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP8986586.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQ"
BASE COUNT         5304 a         3529 c         2895 g         5842 t
ORIGIN      
        1 cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt tggctattct tattacttga
       61 tgcagggcag cttgtgtcaa gacttcttta catcaaactt tttaatttta cattgggata
      121 tttatccgca aaccagcgtt ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa
      181 acggtcacga gccaagatat tgcgacttga actcatcttc tcatccaaat cttctggatc
      241 gatccagcga gcgattttgt gtcccattgg agacatgatt acttcggcgt tatactcatt
      301 taacatacga tattggaata cttcgaattg tagctgacca acagctccaa tgatatattc
      361 ttccgttaga tatgttttat aaagctgaat cgctccttct tgaactaatt gatagattcc
      421 tttgtggaag gatttttgtt tcatgacatt ttttgcagtc actttcataa acagctctgg
      481 agtaaatgaa gggagttctt cataagcaac ttttagtttt ccttcgaaca atgtatcccc
      541 aatctgataa ttgcctgtat catatacacc gatgatatcg cctgctactg ctttttcgac
      601 gttttctctc gaatctgcca taaattgtgt gacattgctt aatttcagtt ttttatttgt
      661 acgttccaac cagacatcca ttcctcgttc gaatgttcca gaacagattc ggacaaaagc
      721 gattcggtca cgatgggctg gattcatatt tgcttggatc ttgaatacga aacctgaaaa
      781 ttcttcctca taaggactta ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg
      841 tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc
      901 aaaaaatact ggtgtctgat cgccacgagc aattttttct tgattaaatg catctccagc
      961 ctctttgatt agttctactt cttctaagac ttgttcatat aaggaatttt cagttagagg
     1021 atgattttcg ggaagcagtc catcttcatc caattctact aatcgttcat cctgataatt
     1081 ttccggacga taaaactcta cacgattatg gtagatatca taaagacctt ctagcccttt
     1141 acccataccg attggccaat tcatcggata agattcgata tctaatagct cttctaattc
     1201 ttctaacaat tccaatggtt cacgaccatc acgatctagt ttgttgataa aagtaaatat
     1261 cgggatgcca cgttttttta caacttggaa taatttcttt gtctgtgctt cgatcccttt
     1321 tgcactatcg attaccatga ccgcactatc cacggccatc aaagtacgat aagtatcttc
     1381 agaaaaatct tcatgtcctg gtgtatccaa gatatttact cgtttccctt gataatcaaa
     1441 ctgcataaca gaactagtaa cagaaatccc acgttgtttc tcgatttcca tccagtctga
     1501 tttggcaaaa ttacctgttt ttttgccttt tacggtcccg gcttgacgga tagccccacc
     1561 aaacaatagt aattgttctg taatcgttgt tttcccagca tccggatggg aaatgatcgc
     1621 aaaagttctg cgattgtcca cttgttgctt taattttgga ttattcataa cctaactcca
     1681 tttcttccat tcaattattt atattttctc taggcacaga ttgtccgatc tcttaaagaa
     1741 agacaatttc atatagggct gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac
     1801 ggcgttctag agacagccta tcagcaataa gcccacccaa gccaaaaata cgaaaagctg
     1861 tttttgcttg gctgctctta ttacttaatg caggatagct ttttcacaac ctttgtcaac
     1921 tactacaact gaatcagtat agcatggatt cagcgaatga aaaagccacc aaaccataag
     1981 agtcaaatat ttttttgtca gctgactaaa agaaaagacg tgttgcttgt ttattcaagc
     2041 aacacgtctt tcataaccaa gtttccaatt atcttcttgg ctcgtcatct tcgaattctt
     2101 ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa
     2161 tgacacggat tttaaggatt cgagaacctt ccatttcttc agaaaccagc gtaatattat
     2221 cgacatcaaa cgataacttt tcgccttcat ccggaatcat gcctaatgct gtaatcaaat
     2281 aaccagccat cgtatctaca tcactcatat gcagatcact gtcaaaagct tcattgaact
     2341 catcaatcag catacgtcct tggatgatat attcgtgttc atcgattttt tcataaagat
     2401 tctcaacttc atctgattca tcatcgattt ctccgacgat ttcttctaaa aggtcttcta
     2461 gtgttgctaa cccaacgacg ccgccatatt catctaataa aatagccatc tgattctgcg
     2521 tctttttcat ttcatataac agatcatcaa taaagaccgt ttcaggaacg aataaaggct
     2581 cctgcatgat tttcttgata tctaaattat caaagccgaa tttatgggca gcttttagta
     2641 aattctttgt atgaaggatt ccgactactt tatctttgtc ttcgttataa acagggattc
     2701 ttgaatagtt ttcggataaa acttcattca cattttcttc cacagtgtcg ttgatatcaa
     2761 tcataaatgc atccgttcgt gggaccatga cttctcgggc aactttcgta tcaagagaaa
     2821 aaaccccttg gagcatttct aattcttcat tattcaatac gccttcgttt tcaagcatat
     2881 aacgcatttc atctctggtc atttttgaat cttcatcatc aaaagtcatc ggcgtgatac
     2941 gcgccaataa acttgtggaa gcagaaagca gccagacgaa gggcttagct actacaccaa
     3001 tcaagcgtat cagccctgaa gtgaactttg cgacttcttc tgatttattt aaagcaatac
     3061 gtttagggta caattctcca aaaacaatcg atacataggt caaaatagcc agcacaatga
     3121 tatttgcaat attttttgcc gctgtgccgc cacctaatac cggagctaac cgttccgata
     3181 aagtattagc tagtgaagca ccagacaaaa tgttgaccaa agtgattcct acttggattg
     3241 tcgataggaa attatttgga ttctgcaata tttttaaaag cttttgagat ttcacatctc
     3301 cttcatcagc tttttgctcg attcgattct tattgatcga aacaacagca atttcggaag
     3361 ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg
     3421 actgactctc agggtcagca ttcataatcg cattctctcc ttataaaatt aatttttagt
     3481 ttacgataga aaaaaacgtg gcactatagc cacgttaaaa tggtatcaca aagcaatttt
     3541 ctttgcaata aaaacaagtg tactaaaact acctacgagg tcgtagcaat tttcttcttg
     3601 ttttcatttt gcttgtcttc tttgacgatt ttgattatat aggaaatgat cgtcttgcag
     3661 attgcgtaaa aaggaacacc taagaagata ccaagcaatc cggcaatatt tccagctact
     3721 agaagaacga taatgatcgt taatggatgg attttcagtg atttcccaat tacattagga
     3781 taaatgatat tcccatccaa ctgttggacg attagcacaa ccacacaaca aagtaatgct
     3841 tttaccggat cattgaagac tgttactaga aatgcaggtg ctagtcctag ataaggtcct
     3901 aaatatggaa ttaggtttgt caatcccgca atgaccccaa ataagaaagc ataatccaca
     3961 ccaattacca tatatcctaa aaatgtgaag gtaccaacga ataaacattc gattgcttgt
     4021 ccgctaatgt aattagccaa tgttttattc agttgcccca ataactccac aatttggttt
     4081 cttctttttt ctgggaaaaa ctgttggatg tttggcacta gtttttctcc gtctttcaac
     4141 atatagaaca agatgaatgg tgctgtaatg attactacgg ttgccgaagc gatcgttcca
     4201 acgatcgaac ctaaactatt ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa
     4261 gaaatgtcta attgctcaaa atatttattg atatctactt ccttgaacaa aggcaattcg
     4321 gctacctctt ttaaccagct ttctacctgt ttgatgaaat caggcatatt agatgccaat
     4381 gaggaaattt gctgtactag acttgggatg acactaagta aaatccaaat aattgctgca
     4441 actaaaagga gcagcacaat tgcgacagcc catattcttt tgatttttgc tttcatcaac
     4501 agcttgacaa taggattcaa taaatagtaa agaaagcctg caatcagtac aggtgcaaat
     4561 aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt ttatttctga tgaaatcatg
     4621 atcaatgcag ctatgatcaa taattcaagc gaccaaaaca ttagtttaga ctgtctaagt
     4681 ttttcaaaca atagatttcc tcctttcttt cttactagta tatattatat tcgttaaaaa
     4741 ttcttttatt ctttttttat ttttgaaaac ttgccatact ttttatttgt tttattttcc
     4801 agtatactat acgaaaggaa ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa
     4861 tgagcaatat ttacttagat gctgtacgga tcaggcatca agtttttgta gtcgaacagg
     4921 gtgtgccttt gtctcgagaa atagacaaag atgaagccca ttgtatccac ttcgtcctgt
     4981 actcagataa aaaagaaccg caaggaactg tacgtctttt gcctttagaa aacggaaaaa
     5041 tgaaattaca gcggatggcg attctttctg aatacagaca ccaagggtta ggaaaaatat
     5101 tgattgaaga agcagaaaac tttgctaaga atcaaggcta taatacgatt ttattagggg
     5161 cacagtccac agctgaaact ttttacgaaa aactaggata tacagcttac ggcgatcctt
     5221 ttgaagatgc tggcatgcct catatttata tgaaaaagac tttataaaaa tttatttcga
     5281 aaaaagcaaa aaacagcaag cttttgagaa aaatctctca ctggcttact gttctttttt
     5341 gtgtttactt acgcgacaat tgcttgtaac ggttatacca aatatcaata tagccttccg
     5401 agaacgggcc atctccatta ttgatccagt caaccagaat cttcacattt tcttttagga
     5461 tgaagtcaat atcgttcggg taattcatca ttttgctgtg catctcatat tcatcgacat
     5521 ccaatagtcg tttctcgccg tcaggaaata ccttgatatc caagtcatag tcgatatatt
     5581 tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt acaataatag gaaaccccct
     5641 tttctctgat cattgctatt acattgaacc agtatttttt atgaaaataa acaatcgctg
     5701 gttcgcgcgt tacccatcgg cgtccatcag attcagtgac taatgtgtga tcattcacgc
     5761 cgatcataga gtgttcgctt gtttttaata ccatggtatc tcgccacgtt cgatgcaatt
     5821 tgccgtcgtg cttgtaactt tggatcgtca caaactctcc ttcttttgga actcccatag
     5881 caacccttcc ttaagcagca ttttgtgctt tgtgtttggg tgttcattac accgacatca
     5941 taattattat atcaataaaa atgagctttg cctagaaagg attcctattg tttcgatttt
     6001 tctctaactg atctaccagt ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat
     6061 caggcttcaa ccacttcgaa gttttgttct cggtaaattc ttgttctgct ccctcggttg
     6121 cccgtccgta aaaaaggaga acatgccatt tcagatgact aaaaatatga gtaacttctc
     6181 ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc aaatgggaga ttttgctctt
     6241 cttctgctac taggtcatca aacagatcga tttctggctt tgtttcccat tcttttttca
     6301 atcgctcata ctcctcttgt gtcacttcca tcattggaaa tgtccacata ttcgccagca
     6361 atttttggct atctctttct tcaaaataat acgctccaga atgattttga agagctgcac
     6421 tgatataata aacgtctttt ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga
     6481 ttcctcgttt gttagctaaa caaaaggctt ggatcggaca agtctcacac tttggagaag
     6541 ttggtgtaca gatagctgaa cctagatcca tcattgcttg gttgaattca cctggatgct
     6601 tttcatcaat gattttccgc atcgcttcat caaagatttt cctgctagaa gcttttgcga
     6661 tgtctgcttc aatgcaaaac aatctgctga ctactcgcat cacgttgccg tcaactgcag
     6721 gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt cgtatatggt cctattccct
     6781 tcaatgagct aatttcttca ggcgtttgcg gcatttcccc atcaaattct gacatgatct
     6841 gtttagcagc agcttggatg ttccgggctc ttgagtaata accaaggccc tcccatgctt
     6901 ttaaaagctt ttcttctgga gcatttgcta gctcttcaat tgtaggaaac cattccatga
     6961 aacgataaaa ataatcgatc actgtgtcta ctctggtttg ctgaagcatg atctcagaga
     7021 tccagatacg ataaggatca cgattatagc gccaaggcag gtttcttttt tcttgttcat
     7081 accattgaat gaactgatct tgaaattctt ttgtttcttt atctgtccat ttttcccatt
     7141 ctttattcac tttcctcttc cttttccgta atgaatcgct ggatcatatc caaatcaaat
     7201 cctttttgat ataagctttg ctttactttt agatttcttt ttttaggttc aaaccttaaa
     7261 tttcttcgcc atagtttatc tccttgtttg actaaataat catattctat ttcttgatct
     7321 gcttcttgcg gaagatcagc tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc
     7381 atcaatgttt gttggatttt tctcaataat tctttttggc tttttccgtg attttttcca
     7441 aacgcttttt cggctgtctg atgtgcattt tgaacttggt cttcaaatgt atattggctg
     7501 agcgcttgtt caatgatttc aggtttcaaa cctttttgct gcatctgctg cgctaatttc
     7561 tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat aactttcccc atagaccaaa
     7621 tcatcgacaa ggtccaattc ttttaagcgg gctacgattt tatttcgatc ttcttgaagg
     7681 atttcctttt cttttaagta tgttcggatt tctttttccg atcgcaactg ataacttaag
     7741 taattcattg ctaggtgaaa accgaaatca gctccgctac tcttcttgat ctcttggata
     7801 gtttcttcgt ctaattcccg tccttttaat aatcgatagc gtactaatac atcttcagat
     7861 acttgtaaag cttctccttc agataaatct acgcgataga aagggccttt gcctttactg
     7921 attctcgtaa cggttaacat aaacatcctc ctcgaacata tattctctat cataccacag
     7981 aaaatccccg taatcaggta ttatatgtta gaataaacca cagaaaaaag aaaaagggag
     8041 atacttatgg cagaaacaat tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt
     8101 ttaggcataa atggtgaagg aatcggttat ttcaaacggt tgatcatatt tgttccatat
     8161 gcactaccaa aagaagaagt actagtgaaa atcacgaaag caactcctcg ctatgcagaa
     8221 ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt
     8281 tactatgaat gcggtggctg tcaattacaa cacttagctt atcaagctca attggatttt
     8341 aaaaaggatc tgcttttgca agcattggaa aaattcaaac cagcaggctt tcgatcttat
     8401 caacttttgc cgacgatcgg gatggatgag ccttggcatt atagaaacaa agcacaattt
     8461 cagttacgaa aaaataagca gactcaaaaa attgaagctg gtttatacga agcaaactct
     8521 catcagttag tccctataac agactgcttg gttcaagaac ctcgaacaca aaaagtaatg
     8581 aatactgttg ttcaactatt gaataaattt gatgcaccca tctatgatga acggacaaac
     8641 agtggaatct tccgcacaat catggttaga atcggtgtta aaactgggga gttacaagtt
     8701 gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg caatgatccg tgagatcaat
     8761 gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc
     8821 gtgatggggg atgaaacgat ccacttatgg ggaaaagaag ccattgaaga acaaatcaat
     8881 gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa
     8941 ttgctttatg gcgaaggaat caaagcatta gatgtccagc ctaatgaaac gatcgtggat
     9001 gcttattgcg gagttgggac gatcggctta agtgtagcaa aacaagccaa agaagtacgg
     9061 ggaatggata ccgttcctca agcaatcgct gatgcaaaaa aaaatgcgga aagattagga
     9121 tatcacaaca ctcgatatga agtcgggaca gcagagcaag ttctgcctaa atggttgaat
     9181 gaaggttttc agcctgatgg tatcatcgta gatccaccac gtacgggttt ggataacgca
     9241 ttgatccaat cattattgaa ataccctcca aaaaaacttg tttatatctc ctgtaatgtt
     9301 tctacacttg ccagagatct ggttaattta agtaaaaagt tcaagattga atatttacag
     9361 tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg tcgtgaagat gacgagactc
     9421 gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc
     9481 aaacaaaaat agctcttcat gttttatgaa tactgtactt gtgaccgaag agttaacagg
     9541 aggacactat ttatttaaaa aaagggttgt gacatagttt ttgtcacagc cctttttgat
     9601 tattattgga tattcacgtc tatcacttga taaaatgcat ttgtcgtatc gctgatattc
     9661 catactgcta aaatcacatt atatcctttt cgatcctgcg gaagtgtgat cgtttggttg
     9721 actgtttttg gtggaacagt tgctttgtcg tctatttttg tgatcaattc aaaatcacta
     9781 aatttcaaag gttgattagg attccaagtt ggtttggtaa tgtagtaatc ccaagtactt
     9841 gtccgatgtt gtgctgttaa attccaagta atatttaatg ttcctggatt aaccatgttt
     9901 ttatgccatc tgttagatgt ctgttcatct aaaggctcaa agccgctgac tcctgcacta
     9961 gcaatttttc catcaatgaa tgtatttttg gtagcttcga tactttgcgg ttcccattgt
    10021 gctcttccga cattttgatt taagtttcct gctgctgatg ttcctaggta tgcacggctt
    10081 cctggagaag taatataacc atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa
    10141 ctaactaacc caataaacaa taacgtacta atctttttca tgtagaaacc tccttcattt
    10201 gataaccaca ttgtaaacgc tttattgtat taacacaaac attatgcttt tataatttta
    10261 tgttgaagta aaaaaggtga aaaaagtaag atgcagatta taacagaagt agacgagaaa
    10321 agtacaaata gaaaacatga gaatcaaatc atatgatctt tttcaaaaac atcattcata
    10381 taaacaaatg ttcaactttt taataacaac aattatcaat ttttttctta cttcacaaac
    10441 taaatacagg tcttgctgtg agccttcatg gtaaactaat attatagaaa ggagttagta
    10501 aaatgaagat gataaaagat attcgttttt gggtttgtgt tataggaatt gtcatattag
    10561 gctttttatc agggttgctt agcggaaatc ccggaaaata ttactattct ttgcaacttc
    10621 caccatttgc accacctagc tggatatttg ggccgatgtg gaccttactg tatatattga
    10681 tgggtatttc tctttatttg ttacttaatc acaacaacaa aaaacagcga aacaatcttg
    10741 tcggtctttt tgtgattcag ttcattttta attttatatg gtctgctcta ttttttaatt
    10801 taagaaatat tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc
    10861 ttatgtatca gttgtggctg catcatcgcc tggcaatgtg gctcatgatt ccttattatt
    10921 tatgggtact attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa
    10981 aaagagacgt cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc
    11041 taatttaggt ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc
    11101 catactcatt attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat
    11161 aactttgagt atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat
    11221 aggtattcgg atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt
    11281 acccttcact attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga
    11341 acatcattga agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta
    11401 attgtttctt cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt
    11461 agatatcatt gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg
    11521 ccggggtgta gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat
    11581 tgttctcaac tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt
    11641 tattgacgat tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg
    11701 gatcttcgtg tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac
    11761 ctactggtac atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac
    11821 cgaccgcatt gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag
    11881 gaatatcgag cagttgctca attgaatcca tggccatttc gacaccacca aatgcgtagg
    11941 catgatttaa tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca
    12001 aactgatgat cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag
    12061 aacgtccttg ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa
    12121 aaggttcttt gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt
    12181 ttttagaaat tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg
    12241 ataatccggc aatcagcaaa acgaataccc ctaacaaggt taatagaact ttttgccatc
    12301 gcttcataat acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat
    12361 cagcaaattc actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattat
    12421 tattttttaa aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa
    12481 gaccgaatac acgcagttcg caatcgtttt atcttttttt atgtattttt tccagaaaaa
    12541 gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc
    12601 atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg acaatttctt
    12661 cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca
    12721 tggctcttaa aatgactcga actagtgatt gcattaccat catcttttaa tggatagata
    12781 tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag
    12841 tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct
    12901 ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca
    12961 agtgaacaaa tgcatttact tagccaaaaa atctaaaaac ttcataaata gacaaaaaga
    13021 cacgactctc taagaagtcg tgtctttatt caacaaatta ttataattta gtcacttcag
    13081 cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc tacttcttga ccttcttcta
    13141 ggctcttgaa gccgtctcct tgaattgctg agaaatgaac aaatacgtca tctccgccgt
    13201 ctactgtaat aaacccaaat cctttttcat tgttgaacca tttaaccgtt ccgtgttcca
    13261 taaatgtata tcctccaaaa aagttcttgc aagtaacctt gcaagaacat tatacccaat
    13321 ttagaaaacg ctagcaagct ttcgctcttt tattttccaa attattttta agactgatat
    13381 gacggaggaa atggttattt tgctgcttcg aaacgtttgt ttacttcatc ccaattaata
    13441 acattccaga atgctgcaat ataatctggt cggacatttt tatatttcaa ataataagca
    13501 tgttcccaaa cgtccaaacc tagaattggt gtctttcctt ccatcaatgg agaatcttga
    13561 tttgcagtag aggtaatagc taattttcca ttttccatta caagccaagc ccatccagaa
    13621 ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa aagaagaaaa atcaccaaaa
    13681 gcttcattaa tcgcttcttt tatttctcct gtaggttcgc caccagcatt tggtgccatg
    13741 atttcccaga aaaatgaatg gttagcatgt ccgccaccat tgttacgtac agctgtcttg
    13801 atatctgttg gaatagcgtc catatcagat aataattctt ctattgtttt ttcgcctaat
    13861 tctgggtatt tctcaattgc tgaatttaaa ttcgtcacat aagtattgtg atgcttatca
    13921 tgatgcagat gcatcgtttc ttcgtcaata taaggttcca atgcgtcata tgcataaggt
    13981 agatctggta atgtgtaagt cataaaaaaa ttcctcctca atgttttctt aataatgtct
    14041 acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt gttatgccta gtcgtctttt
    14101 tcactttgac ctacttcttc ttcaaaaacc tcagtttctt taaaaataaa caacttgctg
    14161 aaaatatagt tcgcaaggat aacaataatt tgagtgatga ttttcgccac atactcattg
    14221 ctgttcataa cattgatcaa caataccatt gcccccatgt ccaatccgta agaaaggata
    14281 cggtagaaga agaacttacc gcattcaata agtgcatgtg taaaattcgg tgattttgaa
    14341 tggaataccc aaagtttgtt cgttacaaaa gcaaacaata cagaacaaaa ccacgaaata
    14401 gaattggata aggccatact taaatcaaaa tattggcgtg cgagagcaaa actaacaaaa
    14461 tttacaatcg tagccaaacc accaaaaaag agatagacta gtatctccca atatcctttt
    14521 gcttctaaat atccttttaa ctgtcgatac tgcttcatga gccaacctac cttattaaaa
    14581 attcttattt atcatactct atatttcaat aactttcatc tttatttaaa aaaattaatt
    14641 ttttttgatc ctttgccttt gtctctgctt ggatcgtcac atgtgtaata ccatatttta
    14701 attttaataa agaaattata cgaggatata tcttttcaat ctcgcttagt agctgatctt
    14761 caaaatcaat atgtccgtcg aaaataatta tcttttcgct atactgccaa atatggacat
    14821 gatgaacatc ctttacccca tcgattgctt caatttcttg ttccattttt tcatagtcga
    14881 tagcaggtgc actttgtatc aaaatagcgg cagctttttt gatcaccatt atcgcttcta
    14941 tcaatatata tatcgaaatg atcagtgtaa taactggatc gatccagtag atatcgaaaa
    15001 aacggatcaa aattgctccg ataatcacac ttaccgaaga cagtgtatca cttaacaaat
    15061 gcaaataact agattttata ttcaaacttt catctgcttc ttgtcgtaat aataatactg
    15121 agaacaaatt cgcgagcaat ccaataactg ctactgttag catcaaatga ctatttatct
    15181 tttctggtga agtgaaccgc ttgaatgctt caacggttaa aaacaaggat aatatgatca
    15241 atatgaatga gttgatgaaa gcactcaata tttctaaacg ctggtagcca tacgtacgtt
    15301 ttttatttgc ttcttttcgg gccatttttt gtgccccgta agaaaaaata attgccaatg
    15361 tatcactaag gttatggaaa gcatctgaaa caagagacaa actccctgac ataatccctc
    15421 caataaattc tgataaagtg atcaccgcat tcaaaaccac tactccaaat agtttttttg
    15481 atgatctctg tggctgtgta ggcactattc cttcctccat tttatttata ttggatagtc
    15541 tttagacaag aataacattt ttttgatatt tcaccaatca ttcagctgtt tccttaaagc
    15601 acttgtattt cacttgatac ttcgctagaa ttgcaagtga tgacactact agatgaggag
    15661 tacttatgac aacaaaacaa ttaattatca gttcctttat acgattcaaa gacctaaagg
    15721 aggcgagaaa tgtcccattt tggaaagcca ttgtctatct tttagttttg agtataatta
    15781 tggctttgcc aatctctttt caagtttttc aagtcttaaa caacattcaa ttggatgggc
    15841 aaaagattgc tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag
    15901 aaaaagaagg attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa
    15961 aacgaacaga acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt
    16021 taaaaaacaa acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca
    16081 ataaccaatt agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaagcagc
    16141 tccgttcttt tttaagtgaa gcatctatac cactttggat aaaagtcctg acatttatat
    16201 tttctattta tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg
    16261 cttttctcta tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat
    16321 tgatctattc aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt
    16381 cttttgacac tagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca
    16441 aaggatgggc aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca
    16501 atagaccctt ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat
    16561 atttctttct caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta
    16621 gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat
    16681 ttcgtgatga atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag
    16741 aattacaacc agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc
    16801 aagaaaaact attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg
    16861 atcagctacc ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca
    16921 tccacagtga atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc
    16981 tttttcaagc acaaaaggca cagagtgaca tgaaatggtt aggttcatcg gtccattacc
    17041 aatctatcca agggaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg
    17101 caaaaaaatg caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag
    17161 cagaaggaat ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag
    17221 atttcgtagt caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata
    17281 tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta
    17341 agacacaaga tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg
    17401 tttttgagga agagcctctt ccacaagatc atcctctttg gaaaatggac aatgtactga
    17461 ttacttccca cattgcaggt ttgacacctg attttcaaaa gaaattaatg gctatttttt
    17521 taaccaatct gaaaagctac tttgccaccc atgagcttca aaccaatcaa
//
LOCUS       DAZKAO010000055        17227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000055 DAZKAO010000000
VERSION     DAZKAO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.055"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG8H8_001515"
     CDS             3..371
                     /locus_tag="ACG8H8_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP8986587.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG8H8_001516"
     CDS             551..1492
                     /locus_tag="ACG8H8_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP8986588.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG8H8_001517"
     CDS             1561..2292
                     /locus_tag="ACG8H8_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8986589.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG8H8_001518"
     CDS             2436..4616
                     /locus_tag="ACG8H8_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP8986590.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDVLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG8H8_001519"
     CDS             4636..5118
                     /locus_tag="ACG8H8_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP8986591.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG8H8_001520"
     CDS             5134..5895
                     /locus_tag="ACG8H8_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP8986592.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG8H8_001521"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG8H8_001521"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP8986593.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG8H8_001522"
     CDS             7667..8152
                     /locus_tag="ACG8H8_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP8986594.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG8H8_001523"
     CDS             8145..8828
                     /locus_tag="ACG8H8_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP8986595.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG8H8_001524"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG8H8_001524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP8986596.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8H8_001525"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8H8_001525"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP8986597.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8H8_001526"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8H8_001526"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP8986598.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG8H8_001527"
     CDS             13125..13457
                     /locus_tag="ACG8H8_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP8986599.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8H8_001528"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8H8_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP8986600.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG8H8_001529"
     CDS             14283..15656
                     /locus_tag="ACG8H8_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP8986601.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8H8_001530"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8H8_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP8986602.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG8H8_001531"
     CDS             16711..17151
                     /locus_tag="ACG8H8_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP8986603.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5877 a         3010 c         3795 g         4545 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacgtacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatggga
//
LOCUS       DAZKAO010000056        17210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000056 DAZKAO010000000
VERSION     DAZKAO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.056"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1412
                     /locus_tag="ACG8H8_001532"
     CDS             72..1412
                     /locus_tag="ACG8H8_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8986604.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1630..2073
                     /locus_tag="ACG8H8_001533"
     CDS             1630..2073
                     /locus_tag="ACG8H8_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP8986605.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACG8H8_001534"
     CDS             complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACG8H8_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP8986606.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4398..5621)
                     /locus_tag="ACG8H8_001535"
     CDS             complement(4398..5621)
                     /locus_tag="ACG8H8_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8986607.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5652..6491)
                     /locus_tag="ACG8H8_001536"
     CDS             complement(5652..6491)
                     /locus_tag="ACG8H8_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8986608.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6508..7188)
                     /locus_tag="ACG8H8_001537"
     CDS             complement(6508..7188)
                     /locus_tag="ACG8H8_001537"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP8986609.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7190..8263)
                     /locus_tag="ACG8H8_001538"
     CDS             complement(7190..8263)
                     /locus_tag="ACG8H8_001538"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP8986610.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8319..8557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8600..8833)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8910..9581)
                     /locus_tag="ACG8H8_001539"
     CDS             complement(8910..9581)
                     /locus_tag="ACG8H8_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8986611.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACG8H8_001540"
     CDS             complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACG8H8_001540"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP8986612.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12658..12832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13005..13442
                     /gene="ndk"
                     /locus_tag="ACG8H8_001541"
     CDS             13005..13442
                     /gene="ndk"
                     /locus_tag="ACG8H8_001541"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP8986613.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13464..14330)
                     /locus_tag="ACG8H8_001542"
     CDS             complement(13464..14330)
                     /locus_tag="ACG8H8_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8986614.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14490..16583)
                     /locus_tag="ACG8H8_001543"
     CDS             complement(14490..16583)
                     /locus_tag="ACG8H8_001543"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8986615.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4973 a         3567 c         3040 g         5630 t
ORIGIN      
        1 gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa
       61 agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca
      121 tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg
      181 ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg
      241 tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg
      301 gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt
      361 ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt
      421 ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca
      481 tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa
      541 tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg
      601 gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg
      661 gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac
      721 tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca
      781 aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc
      841 tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa
      901 aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct
      961 catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc
     1021 gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg
     1081 ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca
     1141 aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta
     1201 tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca
     1261 gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca
     1321 ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa
     1381 agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat
     1441 gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt
     1501 tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa
     1561 aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt
     1621 ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt
     1681 atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca
     1741 cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg
     1801 tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt
     1861 tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg
     1921 aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa
     1981 gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac
     2041 aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag
     2101 gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga
     2161 ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2221 cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2281 ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa
     2341 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2401 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
     2461 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
     2521 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
     2581 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
     2641 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
     2701 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
     2761 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
     2821 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
     2881 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
     2941 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
     3001 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
     3061 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
     3121 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
     3181 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
     3241 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
     3301 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
     3361 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     3421 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     3481 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     3541 accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     3601 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     3661 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     3721 atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg
     3781 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     3841 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     3901 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     3961 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     4021 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct
     4081 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     4141 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     4201 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     4261 acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt
     4321 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     4381 cacaagatta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
     4441 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     4501 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     4561 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     4621 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     4681 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     4741 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     4801 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     4861 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     4921 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     4981 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     5041 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     5101 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     5161 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     5221 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
     5281 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     5341 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     5401 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     5461 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     5521 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     5581 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     5641 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     5701 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     5761 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     5821 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     5881 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     5941 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     6001 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     6061 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     6121 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     6181 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     6241 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     6301 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     6361 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     6421 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     6481 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     6541 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     6601 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     6661 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     6721 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     6781 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     6841 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     6901 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     6961 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     7021 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     7081 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     7141 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     7201 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     7261 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     7321 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     7381 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     7441 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     7501 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     7561 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     7621 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     7681 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     7741 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     7801 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     7861 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     7921 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     7981 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     8041 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     8101 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     8161 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     8221 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     8281 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     8341 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     8401 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     8461 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     8521 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     8581 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     8641 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     8701 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     8761 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     8821 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     8881 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     8941 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     9001 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     9061 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     9121 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     9181 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     9241 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     9301 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     9361 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     9421 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     9481 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     9541 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     9601 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     9661 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     9721 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     9781 tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta
     9841 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     9901 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     9961 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
    10021 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
    10081 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
    10141 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
    10201 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
    10261 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
    10321 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
    10381 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
    10441 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
    10501 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
    10561 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
    10621 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
    10681 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
    10741 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
    10801 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
    10861 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
    10921 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
    10981 tcagcatttc ttcaatcgca ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
    11041 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
    11101 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
    11161 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
    11221 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
    11281 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
    11341 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
    11401 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
    11461 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
    11521 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
    11581 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
    11641 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
    11701 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
    11761 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
    11821 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
    11881 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
    11941 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
    12001 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
    12061 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
    12121 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
    12181 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
    12241 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
    12301 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
    12361 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
    12421 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
    12481 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
    12541 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
    12601 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
    12661 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
    12721 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
    12781 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
    12841 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
    12901 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
    12961 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
    13021 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
    13081 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
    13141 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
    13201 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
    13261 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
    13321 cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
    13381 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
    13441 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
    13501 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
    13561 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
    13621 atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg tttcatatct
    13681 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
    13741 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
    13801 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
    13861 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
    13921 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
    13981 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
    14041 ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
    14101 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
    14161 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
    14221 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
    14281 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
    14341 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    14401 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    14461 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    14521 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    14581 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    14641 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    14701 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    14761 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    14821 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    14881 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    14941 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    15001 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    15061 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    15121 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    15181 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    15241 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    15301 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    15361 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    15421 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    15481 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    15541 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    15601 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    15661 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    15721 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    15781 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    15841 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    15901 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    15961 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    16021 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    16081 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    16141 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    16201 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    16261 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    16321 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    16381 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    16441 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    16501 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    16561 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    16621 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    16681 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    16741 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    16801 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    16861 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    16921 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    16981 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    17041 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    17101 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    17161 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
//
LOCUS       DAZKAO010000057        16969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000057 DAZKAO010000000
VERSION     DAZKAO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.057"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8H8_001544"
     CDS             <1..1152
                     /locus_tag="ACG8H8_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFP8986616.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8H8_001545"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8H8_001545"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP8986617.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8H8_001546"
     CDS             2728..3003
                     /locus_tag="ACG8H8_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP8986618.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8H8_001547"
     CDS             3009..3776
                     /locus_tag="ACG8H8_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP8986619.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8H8_001548"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8H8_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP8986620.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8H8_001549"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8H8_001549"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP8986621.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8H8_001550"
     CDS             7920..8222
                     /locus_tag="ACG8H8_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP8986622.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8H8_001551"
     CDS             8225..9388
                     /locus_tag="ACG8H8_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP8986623.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8H8_001552"
     CDS             9414..12842
                     /locus_tag="ACG8H8_001552"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP8986624.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8H8_001553"
     CDS             12863..13987
                     /locus_tag="ACG8H8_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP8986625.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8H8_001554"
     CDS             13968..14414
                     /locus_tag="ACG8H8_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8986626.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8H8_001555"
     CDS             14398..14736
                     /locus_tag="ACG8H8_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP8986627.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8H8_001556"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8H8_001556"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP8986628.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8H8_001557"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8H8_001557"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP8986629.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8H8_001558"
     CDS             15893..16936
                     /locus_tag="ACG8H8_001558"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP8986630.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5748 a         2935 c         3749 g         4537 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg cgccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgag
//
LOCUS       DAZKAO010000058        16716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000058 DAZKAO010000000
VERSION     DAZKAO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.058"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..832)
                     /locus_tag="ACG8H8_001559"
     CDS             complement(<1..832)
                     /locus_tag="ACG8H8_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8986631.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="ACG8H8_001560"
     CDS             complement(846..1643)
                     /locus_tag="ACG8H8_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8986632.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="ACG8H8_001561"
     CDS             complement(1676..1960)
                     /locus_tag="ACG8H8_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP8986633.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="ACG8H8_001562"
     CDS             complement(1957..2958)
                     /locus_tag="ACG8H8_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP8986634.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8H8_001563"
     CDS             complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8H8_001563"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP8986635.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="ACG8H8_001564"
     CDS             4026..4874
                     /locus_tag="ACG8H8_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8986636.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5520..5726
                     /locus_tag="ACG8H8_001565"
     CDS             5520..5726
                     /locus_tag="ACG8H8_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP8986637.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5928..6929)
                     /locus_tag="ACG8H8_001566"
     CDS             complement(5928..6929)
                     /locus_tag="ACG8H8_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8986638.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6934..8846)
                     /locus_tag="ACG8H8_001567"
                     /pseudo
     CDS             complement(6934..8846)
                     /locus_tag="ACG8H8_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(9014..9520)
                     /locus_tag="ACG8H8_001568"
     CDS             complement(9014..9520)
                     /locus_tag="ACG8H8_001568"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP8986639.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9680..10120
                     /locus_tag="ACG8H8_001569"
     CDS             9680..10120
                     /locus_tag="ACG8H8_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8986640.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10146..11261)
                     /locus_tag="ACG8H8_001570"
     CDS             complement(10146..11261)
                     /locus_tag="ACG8H8_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986641.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11306..11662)
                     /locus_tag="ACG8H8_001571"
     CDS             complement(11306..11662)
                     /locus_tag="ACG8H8_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986642.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11960..12934)
                     /gene="sgrA"
                     /locus_tag="ACG8H8_001572"
     CDS             complement(11960..12934)
                     /gene="sgrA"
                     /locus_tag="ACG8H8_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP8986643.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(13130..13969)
                     /locus_tag="ACG8H8_001573"
     CDS             complement(13130..13969)
                     /locus_tag="ACG8H8_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP8986644.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14154..15041
                     /locus_tag="ACG8H8_001574"
     CDS             14154..15041
                     /locus_tag="ACG8H8_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP8986645.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15635..16330)
                     /locus_tag="ACG8H8_001575"
     CDS             complement(15635..16330)
                     /locus_tag="ACG8H8_001575"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP8986646.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16314..16712)
                     /locus_tag="ACG8H8_001576"
     CDS             complement(16314..16712)
                     /locus_tag="ACG8H8_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP8986647.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4996 a         3405 c         2574 g         5741 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat accgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taagtaactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa catcaatagt
     4981 cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt attattcgag
     5041 ttacttgtta gatgcgataa caaagttagt atacagtagt aaaaaattta tcttttcttt
     5101 tttttattat ttttgtttct tattttctaa aaaattaaaa aaaattttgg tgaattaagt
     5161 atttccttga taacactggc ttttggcctc attttaggtt atcatttgta attttatttc
     5221 acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa tttttgggga
     5281 aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc tattattggt
     5341 ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa ttgcttatca
     5401 ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct aacaaacttg
     5461 tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag gtgctgctta
     5521 tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta tttgctattc
     5581 atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca gcattctttt
     5641 gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt gttgcttttt
     5701 ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga tcgtgacaat
     5761 aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc taactcctca
     5821 gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt gctccttgtg
     5881 acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta ctccttgaaa
     5941 aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc aaactgatat
     6001 tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt cacccccgct
     6061 ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt atttccatcc
     6121 gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa acttttgacc
     6181 ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt ttcataaaga
     6241 aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag accagacaaa
     6301 gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc agctttttta
     6361 atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc tgccgaattt
     6421 ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt agctgcttga
     6481 atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga tcgttcataa
     6541 tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata atcacgacaa
     6601 tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat cggtaatttt
     6661 ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt tgaaggatag
     6721 gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc tggtcgggca
     6781 tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct aatcggcttt
     6841 ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat aactggtact
     6901 gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc gccaaatcca
     6961 tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg aatgtttctt
     7021 ttctccagtt ttcggcaaca ttcttttttt gcatctgtct ttttttgtac tttcactgtc
     7081 atctgaatcg gttgcgtatc atcacttgat tgatcctctc gaggttcatt ctttccctta
     7141 ttgtctgcat tctctgtcgg atcttgcttt tttggtggag ctactgggat ttttttgtaa
     7201 tagaaaataa tagttggtat cgtctcatta aaaatccctt cagtcggtcc ttcaacattt
     7261 gctaattcgt atccttcgaa tgtcttttgc tctgtcatat aattttctcc ataagctccg
     7321 taaagctgga tttcgtcctg gtctttgagt agacttttat tgttttcatc cacatacttt
     7381 acgtttatct gatcatggaa cactactttc aaaaagtccg ttacatgttt cgtgtaggca
     7441 gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt ctactaataa aagatcttta
     7501 taccctcctg ataactcata taatttttgc gcgttagaca caggtactac atcatctgct
     7561 gttccatgga taatcaattt aggcaatgaa tctgacattc caatcgtagg tagagcagct
     7621 tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa
     7681 tcttcatgct cttgatcccc aatgatgtct aactgatttt ttatggcatc cggtagtgcg
     7741 ggggctactg tctggctgta cgtaaaacgt agttgttcat caattgatgc aaatccgcaa
     7801 ttttcaataa ttcctttgac attgacagga atatctttcg ctaatacaga catcatcgta
     7861 gctgctccca tcgaaccacc ataaagtaaa atcttttgag atggccgctc tcgaacttct
     7921 tgatttatcc agtaaaggac gtcatctgac tcatattgtc cataggtcac ataatcgcct
     7981 ccgctattac cagtcgctct ggaatcaaca aataataaat tatacccggc cttataaaaa
     8041 tcattgtatt cttctgtaac gatcccgtta ttccaattcc ctcgaaatcc gccatggatc
     8101 atgactgttt tatctgaacc ttgatcgaca taatatgcat atacatccat cattttgcca
     8161 tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt
     8221 tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg acatcaacac actgtttttt
     8281 gcaaaagcga tatcgtacaa tagataagta gcaatcagat gtttgataat ctctttatct
     8341 acaccataat cattaacagc aagctcagtg actaataact ctattaattt tgacttgatg
     8401 tcttctgttg gtccattaag tatttctgtc atctgtttac cgaagtaatt caaatattct
     8461 ttaccattga aattttcacc tttaccgata tcatcatttt ctgtcccatt ctctttgtca
     8521 cttactttag cagaagagga ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt
     8581 tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc
     8641 gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca
     8701 gtagtttctg tggtttctac ctcccgagat tccgattcgg aagaaatgct ctctccatat
     8761 actgtccatt gcgggacgct tgtacttaca gcaaataaga tgaccaacgc tccctttaat
     8821 acttgttttg ttctcaagct attcactcct ttttatgttt ctaggagaat tatatgtaaa
     8881 cgcaaacatt ttttatacaa aaaataaatg gtttatccca taaaaataga acaggattat
     8941 tttctcattt tacgtaataa agcagaatgg ataaaccagt atccattctg ctttttttaa
     9001 acgaagaaac aacttattgc tccaaaaatg tcagaaatcg tttatcccaa attttctcgt
     9061 aaaaagcgat tgtttccttt gctttcataa aaggattatc ataagttgtc gttgctcctc
     9121 tacacatcat aagctggtat cctaatccat gagcaaactt cacagtcgta tctatacaat
     9181 actctgcttg tgccccacat aattcgatcg aatgacagtc caattcagtt aataaaacac
     9241 tcaaattagt cttataaaat gaattcgcat gggtttttct cacaaaataa ctgtgctcag
     9301 gaatagataa ttccgacaat agctcccatg gttttgtttg tggcactaat tgagcatctt
     9361 cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta tgttttgatt cttttattca
     9421 ctaatgctgt cactgtttct aattcgtgta tcggcgaaga atcaaaacat accccttttt
     9481 gtaaatcgat cactactatt gcgtcggctg tgtttgacat acttgtgcct tctttctttt
     9541 ttcttgattt aatagtattt cttatctagc tagttccatt atacgtgaca acattttctt
     9601 attttacacc tctaaatggc acttttcgac tatttcttca tgttattatt tcataaatac
     9661 ttagctattg gaggaatgta tgatgattaa attgtataca ggttctggct gctcgtcttc
     9721 aagacgcgca aaacgttggc ttatcgtcca tgatataccg tttatcgaac agaacttaat
     9781 tcgtcaacca ctaaccaaaa atgaatttat ccacttacta aaattgacaa acaacggcgt
     9841 gaccgaaatg ctctctactc attctattag ttataaaaat cttgttcaca gaatagatga
     9901 actcagtttg agtgaactat atattgagat tcgtacatcc ccttctttat tgaaaaaacc
     9961 gattatctac gaccaatctg caaaactttt gactggattt agcgaagata aaatcaggat
    10021 gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct
    10081 tgtcgatttt aaagaaaata atctttttgc aatgtcttaa ttttggcata aacaatctgc
    10141 ttttcttaat taacgaaatc caatttttcc aatatggatc tatttttctc attcctgatt
    10201 tttatgattt cagctttcag acgcgcccaa tcattttttg taggttgtcc attccagtaa
    10261 aatatctgtg tattcgtttg atcatatgcc tttttgtcga taggataatc gctaatgaca
    10321 atatcaggta actgatcagc ggatgcacta tattttattg ggcgttcagt catagataat
    10381 atccttttct ttgcccataa ttcctgtgtt ttaccatact ttgattgaat atagaatatg
    10441 acaggttgtt cgtgttttat aaaaatatcc ctgactaaca tacagtattg aaaaatcaac
    10501 cgtctgttct tttcataata agtattaagc tctggaattc tgcgagctac cccactaaaa
    10561 aactgttgca tgattggata catcactgga aatatatgct gaaaatctct gtcttctgct
    10621 gattttccaa aagcatccac cttctttttt gtcccaaaaa cccttgaata caaatggatc
    10681 attgccaaat tgacatatag ataatttttt tctgaagttg taaaacaaat cttgaagttt
    10741 ttttcaaaat tttctacaat agacgattca atccagcccc aactttcttg tactttatct
    10801 ggagtatcca acaaatcttc ttccaaatag gtgttcatta caccaaacat aaaataaagg
    10861 taagcttgct ctgttggagt gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc
    10921 tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg gatgcacaaa tttgttcatc
    10981 tcttctggga catacgtaat cgagtggcct tgtttcattc gttgcaacga aattcttagt
    11041 aggctgatcc atttctttct atctatacta cgcgtatatg gcgcatgttt cgcaaatttt
    11101 tctaatagtt gacgtttatt ttttatactt gataatatat cagaatgatt ttcgtcgaat
    11161 gactgccaat acatcaagtg aaaaaagtat cggatacggt attcttctcc ttctatcgga
    11221 ttaccttttt tcaagctgca agataaatga tagtttttca aaatagtatt aagttctctt
    11281 aattttcttt gaatcgtacg caaacttata taattttttg actaagattt tctaaggatt
    11341 gataagtacc tttgtaaagt gaatccagta aaatataacc aatcgttccc tctaaaagta
    11401 tagaataaac tttactcagt aaaaactgag aagaaaactc gacttttatc atcttgtttt
    11461 ctgaaagaaa agataattta tattctggat acctttcgca aaacgattct gcttgttgaa
    11521 tcaccgaatt catcttagcc agggtgtagt ttagatctaa agcgatatcc ttcaccacat
    11581 ataatttctc gtctctttca taacaatagt tcaaaaattg atatacttgc ctttcattga
    11641 tatcaataaa ttcttcaaac aaagtacata ctccttttta tctattattt agacttattc
    11701 cgacagctac atgaataacg tacaaataga ttcgttactg atttttcaga gatactacca
    11761 tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc catttgtgtt cctgttattt
    11821 cctgtttctc aaatatgtta gtaaacaaaa atagatctgg caaagctaca acttcgccag
    11881 gcaatacagc actctcgaat gaaatattca ctcaaaaatc tatcgtttta ttcaatttta
    11941 gatctgttgc ttttattctt tatttcactc gatggaagag agaacgaaaa tagcttgctc
    12001 ctacaatcaa aatgagtaac acacccatcc atgtaattag cttgttcaag gttctactac
    12061 cagtttctgg taaatcgctt tttctattat ctgtttcttt cgctttttct tgatgatcaa
    12121 ttccacgaga aacaaaatca ggctgtttta gcacactttt taggttgctc cctgcatttc
    12181 tcttttgttc cttagttggt atgataggta ccaaatcaag ttcattattt acttttcctg
    12241 tgctgctttc gctgctcgtg cttcctgtgc tactctcact actcatgctt cctgtgctac
    12301 tttcactact cgtactctcc gtactgcttt cgctgctcgt gcttcctgtg ctactttcac
    12361 tactcgtact ctccgtactg ctttcgctgc tcgtgttttc tgtgctactt tcactgctcg
    12421 tactctctgt actgctttca ccgctcgtgt tttctgtact gctttcacta cttgtgctat
    12481 ccgtactgct tggcggtcca acaactgtcc aactgcttgg tttcaaattc tccgtgatcg
    12541 tcatctgtcc atcataaaaa acaatcaaat caactttatc tttactcttg atctcatcaa
    12601 tcgatagttc tgcagaaatc attattgtat actcaatacc agcggtccat tgtacgtctt
    12661 ttaaataaac ttccccaata actttttctg aacttccatt accatcccaa ccatatgtta
    12721 gaacagcatg tacatcacta gcattcacgc ttttgtttgg tttgactgga atagtaatga
    12781 cttgttgtgt catgtcataa acaatccgat cagttttcac agtaaatgaa agattctcat
    12841 ttgcccgttc atttgcagaa gtactgattc caatcattgc tgcaattata ctcagcccaa
    12901 gaataacaaa taatacggtc gctgttttct tcatttctat cactctccat ataattacct
    12961 tctatttgat aaaggaatta tataaaatga aaatgatgag aacacgacag tcagttgtga
    13021 aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt tactttttcg tttaatcaat
    13081 gacagactta aaaaagcaag accgatatca gccttgcttt cttcatcttt tattgataaa
    13141 cagcagtgta tttcttcatc cgctttgttt tatatgcata gagtaacaaa ccaacaatca
    13201 agactaacaa gaataatgta gttgatatag atgcttcagc cccaaagttt cccccagtaa
    13261 ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc ttgttcttgc gttccgctga
    13321 ctggtatacc caaaatagct ccttgtgtcc aattccaagt aatatgagca ccaatagccg
    13381 taaataaact tcctgtactg tagataagtg cgctgaaaaa gcaacctgct aaaaagatat
    13441 tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg caaaagagca aataatgcag
    13501 cagatccaat cattgctatt aacggaccaa atctaagcat ctgatgctga aaaaagccac
    13561 ggaataaaag ctcttctgat gcccccactg caataaataa aataaaactc gttatcagcg
    13621 gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc cgtcaaatat aatggactag
    13681 taataagcag aaacactcca attccaacaa cgagacctcc cgctaaatac ccccagtctt
    13741 ttcctcgaac agaaacacca taatcttcta ttctttttcc ttcgacctta ctagcaagaa
    13801 ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt
    13861 cactatttcc aataaacata tccgcaagtg cattattgac aagaattcct aaaacaaaac
    13921 caactaagac tattaaaaca gatccaatca accgaataat cctgatcatc tttcatccct
    13981 cctttatatt aagtttaaca acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt
    14041 ctccatcata gagagaatag atttttacga actaagaata tctacagcaa ccttacgaaa
    14101 atgaaagact gtctccctaa ttcttctgtt agactgtttt taataaggaa aggatgggaa
    14161 attttttgaa aaaacaacta ctaattatgt gcctttcagc atgtgcaatt ttactattct
    14221 caggttgtag caagtccaat gaaacgattg caacgtttaa aggtggaaca cttacagttc
    14281 aggattttta tgataaccca aaagttaaat caaataatca aacactatta aaaaaaatga
    14341 ttgtcttcaa agcatttgat gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa
    14401 aagaatataa tgaacaaatc aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag
    14461 ttggccaaac agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg
    14521 gtttgaaagc caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct
    14581 atccagaagt tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa
    14641 aaaaagaagc aaatgaagga gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata
    14701 aaacattaaa ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta
    14761 ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg
    14821 taacagatcc aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg
    14881 ataaaggttc taataaagac aaatacaaat ctgagctaga aaaaattgct accgaagcaa
    14941 aaactcttga tacggaattc atggataaaa caatccgtaa agtgatgaaa aaagacaatg
    15001 taactattaa agatccttat gtaaaaaata tattcaaata aacaaatttg acccaattgg
    15061 ggatgtggca aaatttgaga agtgattttt ggaaattcct tcttattttt gaggcaaacc
    15121 acttttgcca caacttcatc ggggcctttt tttatttgta ccaaaacaaa tcactggatt
    15181 tgttacaatc atgaggatat aatttaaaaa agttttttat ttctatcaca tgaatcaatg
    15241 cgccattaag tagattcttt catgcttccc tattttttca cttgccgtat cgcttcaaaa
    15301 acaactatca taaactcaaa ctcgactgaa aagttatttt aaaaaagact aaaaatatcc
    15361 cttctggact tgttcttctt attctgttat actagagaaa cacgaaagat atttttatgc
    15421 gacacttttc atttgttttt atgaaattta ttcaaaactg gcgtaacttc agagttacaa
    15481 ggatgtatta gaggttaggg aatacatcgt ttaaatgaga aataagtaat agttagtaat
    15541 agtcacccaa actttaaatt aatgaatatt gaatcgtcag atagcctcct cgagttttcg
    15601 agaaggctat cgtttatttt atggagaaat tgattcagaa gtaatcagcg acctttttta
    15661 gttcaccaat cgtttctgct tggaaagttt gcacccaatc ttcttggaag cccctcactg
    15721 ctttatcaat cgagggcatc tctaatactt gatctagtag ctctttacct aacgttatcg
    15781 catgagctcc agatcgaatg gctgtagtga cttgttcaca atttttgaaa ctagctgcta
    15841 atatttttgt tggtctttgc gtacgttcga tctcagtccg caacgaatca atcactttca
    15901 tagcatcaat gccttgattt tgcatcctgt tataataagg tgcaatataa tctgctccat
    15961 tattaatagc taaataccct tgtaattctg tgtaaatagc tgtaccggta atcaaataac
    16021 cttctttttt caactttttg atcgcttgaa ggcctgcttg attcaccggc accttgataa
    16081 acgtatcttt tcccagttct tctatgataa ctcgagcatc ctctatcatt tcttcttccg
    16141 ttcttcctat tgtctgaata tggataggaa tctgtccgat tatttttttg atttctttca
    16201 tatgcgagaa gaagtttgac gctccttctt ttttcagaat tgtaggattt gttgttacgc
    16261 ctgttaaatt caaaaactgt tgacaagaat aaatactttt tgtatttgct gtatcaagca
    16321 tgatttccat atagctttcc cccattctga tcctcttgat cgattttaat gattgtcccc
    16381 tttcttaaat gcggcatttc cttatcttct acatacattg ttcctggaag ttccggctca
    16441 tgagatccat caaattttat cgtgatatgt cctaattcat tcaaattagt ttggacaaca
    16501 tctcctactg ctgtgattac atacatttgt ttatcaaaac agagcttcat atcacaagtt
    16561 atttcttttc ttacctgatt caattcaatc ttataacaat aatctgttaa tgtttccggt
    16621 gcttcatctc caaataaaat cattattttt tctttcctga atacttctgc ttctatacca
    16681 acatctgtta caaccgtttc aaagatcgtc atctct
//
LOCUS       DAZKAO010000059        16699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000059 DAZKAO010000000
VERSION     DAZKAO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.059"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG8H8_001577"
     CDS             <1..546
                     /locus_tag="ACG8H8_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8986648.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ACG8H8_001578"
     CDS             735..1403
                     /locus_tag="ACG8H8_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP8986649.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1923)
                     /locus_tag="ACG8H8_001579"
     CDS             complement(1603..1923)
                     /locus_tag="ACG8H8_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986650.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1960..3342)
                     /locus_tag="ACG8H8_001580"
     CDS             complement(1960..3342)
                     /locus_tag="ACG8H8_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP8986651.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="ACG8H8_001581"
     CDS             complement(3498..4685)
                     /locus_tag="ACG8H8_001581"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP8986652.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="ACG8H8_001582"
     CDS             complement(4692..5723)
                     /locus_tag="ACG8H8_001582"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8986653.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="ACG8H8_001583"
     CDS             complement(5769..7193)
                     /locus_tag="ACG8H8_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP8986654.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="ACG8H8_001584"
     CDS             7368..8273
                     /locus_tag="ACG8H8_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP8986655.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="ACG8H8_001585"
     CDS             8364..9041
                     /locus_tag="ACG8H8_001585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP8986656.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="ACG8H8_001586"
     CDS             9038..9208
                     /locus_tag="ACG8H8_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986657.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="ACG8H8_001587"
     CDS             9322..10218
                     /locus_tag="ACG8H8_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8986658.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="ACG8H8_001588"
     CDS             complement(10273..11643)
                     /locus_tag="ACG8H8_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8986659.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="ACG8H8_001589"
     CDS             11881..12627
                     /locus_tag="ACG8H8_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8986660.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="ACG8H8_001590"
     CDS             12624..13463
                     /locus_tag="ACG8H8_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8986661.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="ACG8H8_001591"
     CDS             13464..14414
                     /locus_tag="ACG8H8_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP8986662.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="ACG8H8_001592"
     CDS             14555..15307
                     /locus_tag="ACG8H8_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986663.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="ACG8H8_001593"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="ACG8H8_001593"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP8986664.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5435 a         3105 c         3343 g         4816 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc agtaattgtt gtaacaatta ctggtttttt tgtatttttt acattttttt
    16561 aagagaattt gataaaaatc aaaaattttt ttgctttttt ttatggatta ttgcagtgaa
    16621 tgttacagaa tctgcgtttt tcattcaaaa ataaaaaaac atagaaaaac gattactttg
    16681 cttatacaac aaggctttt
//
LOCUS       DAZKAO010000060        16682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000060 DAZKAO010000000
VERSION     DAZKAO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.060"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="ACG8H8_001594"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="ACG8H8_001594"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP8986665.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="ACG8H8_001595"
     CDS             complement(2423..3118)
                     /locus_tag="ACG8H8_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP8986666.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="ACG8H8_001596"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="ACG8H8_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP8986667.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="ACG8H8_001597"
     CDS             complement(4317..4508)
                     /locus_tag="ACG8H8_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP8986668.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="ACG8H8_001598"
     CDS             complement(4529..5419)
                     /locus_tag="ACG8H8_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP8986669.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="ACG8H8_001599"
     CDS             complement(5406..6173)
                     /locus_tag="ACG8H8_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP8986670.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="ACG8H8_001600"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="ACG8H8_001600"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP8986671.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="ACG8H8_001601"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="ACG8H8_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP8986672.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="ACG8H8_001602"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="ACG8H8_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP8986673.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10744..11127
                     /locus_tag="ACG8H8_001603"
     CDS             10744..11127
                     /locus_tag="ACG8H8_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986674.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(11295..12053)
                     /gene="jag"
                     /locus_tag="ACG8H8_001604"
     CDS             complement(11295..12053)
                     /gene="jag"
                     /locus_tag="ACG8H8_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP8986675.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="ACG8H8_001605"
     CDS             complement(12071..12889)
                     /locus_tag="ACG8H8_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP8986676.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="ACG8H8_001606"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="ACG8H8_001606"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP8986677.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="ACG8H8_001607"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="ACG8H8_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP8986678.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14018..15352
                     /gene="dnaA"
                     /locus_tag="ACG8H8_001608"
     CDS             14018..15352
                     /gene="dnaA"
                     /locus_tag="ACG8H8_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP8986679.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15550..16680
                     /gene="dnaN"
                     /locus_tag="ACG8H8_001609"
     CDS             15550..16680
                     /gene="dnaN"
                     /locus_tag="ACG8H8_001609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP8986680.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4594 a         3533 c         3001 g         5554 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg cggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggagtc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaatggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggtg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatcta tattttttat ccacagatgc
    13801 aagaggaaat ctattgattt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    13921 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    13981 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    14041 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    14101 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    14161 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    14221 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    14281 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    14341 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    14401 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    14461 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    14521 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    14581 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    14641 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    14701 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    14761 aaacgagatt ccgaaactgc aagaacgcct cgtttctcgc tttgcatggg ggctgtctgt
    14821 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    14881 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    14941 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    15001 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    15061 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    15121 gaaagatatc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    15181 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    15241 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    15301 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    15361 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatgcacac
    15421 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    15481 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    15541 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    15601 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    15661 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    15721 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    15781 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    15841 ttagaaaata aacaagtagc gattacttct ggaaaagcga attttatcgt aaatggatta
    15901 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    15961 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    16021 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt ggctgtagct
    16081 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    16141 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    16201 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    16261 ttctattccc gtttgctaga aggaaactat cctgatacca atcgtttgat tccttcaagt
    16321 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    16381 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    16441 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    16501 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    16561 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    16621 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    16681 ca
//
LOCUS       DAZKAO010000061        16672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000061 DAZKAO010000000
VERSION     DAZKAO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.061"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8H8_001610"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8H8_001610"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP8986681.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8H8_001611"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8H8_001611"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP8986682.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8H8_001612"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8H8_001612"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP8986683.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACG8H8_001613"
     CDS             2926..3531
                     /locus_tag="ACG8H8_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP8986684.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="ACG8H8_001614"
     CDS             3856..4179
                     /locus_tag="ACG8H8_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP8986685.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="ACG8H8_001615"
     CDS             4361..5731
                     /locus_tag="ACG8H8_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP8986686.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="ACG8H8_001616"
     CDS             complement(5861..7162)
                     /locus_tag="ACG8H8_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP8986687.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="ACG8H8_001617"
     CDS             complement(7392..9383)
                     /locus_tag="ACG8H8_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP8986688.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="ACG8H8_001618"
     CDS             complement(9527..9793)
                     /locus_tag="ACG8H8_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8986689.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG8H8_001619"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG8H8_001619"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP8986690.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG8H8_001620"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG8H8_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP8986691.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /gene="def"
                     /locus_tag="ACG8H8_001621"
     CDS             13137..13700
                     /gene="def"
                     /locus_tag="ACG8H8_001621"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP8986692.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="ACG8H8_001622"
     CDS             complement(13953..14474)
                     /locus_tag="ACG8H8_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP8986693.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="ACG8H8_001623"
     CDS             complement(14477..15319)
                     /locus_tag="ACG8H8_001623"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP8986694.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="ACG8H8_001624"
     CDS             complement(15312..16037)
                     /locus_tag="ACG8H8_001624"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP8986695.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="ACG8H8_001625"
     CDS             16213..16509
                     /locus_tag="ACG8H8_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986696.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4688 a         3473 c         3080 g         5431 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag ta
//
LOCUS       DAZKAO010000062        16661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000062 DAZKAO010000000
VERSION     DAZKAO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.062"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8H8_001626"
     CDS             complement(99..1229)
                     /locus_tag="ACG8H8_001626"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP8986697.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8H8_001627"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8H8_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8986698.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8H8_001628"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8H8_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP8986699.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8H8_001629"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8H8_001629"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8986700.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8H8_001630"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8H8_001630"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986701.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8H8_001631"
     CDS             4880..5692
                     /locus_tag="ACG8H8_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986702.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8H8_001632"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8H8_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986703.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8H8_001633"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8H8_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986704.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8H8_001634"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8H8_001634"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP8986705.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG8H8_001635"
     CDS             complement(7059..7937)
                     /locus_tag="ACG8H8_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8986706.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG8H8_001636"
     CDS             complement(7976..9094)
                     /locus_tag="ACG8H8_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP8986707.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG8H8_001637"
     CDS             complement(9091..11190)
                     /locus_tag="ACG8H8_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP8986708.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG8H8_001638"
     CDS             complement(11241..12062)
                     /locus_tag="ACG8H8_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8986709.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG8H8_001639"
     CDS             complement(12059..12868)
                     /locus_tag="ACG8H8_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8986710.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG8H8_001640"
     CDS             complement(12889..13383)
                     /locus_tag="ACG8H8_001640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8986711.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG8H8_001641"
     CDS             complement(13395..13895)
                     /locus_tag="ACG8H8_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8986712.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG8H8_001642"
     CDS             complement(13960..16656)
                     /locus_tag="ACG8H8_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8986713.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         5061 a         3595 c         2686 g         5319 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt t
//
LOCUS       DAZKAO010000063        15819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000063 DAZKAO010000000
VERSION     DAZKAO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.063"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..1830)
                     /locus_tag="ACG8H8_001643"
     CDS             complement(55..1830)
                     /locus_tag="ACG8H8_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP8986714.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(2041..2943)
                     /locus_tag="ACG8H8_001644"
     CDS             complement(2041..2943)
                     /locus_tag="ACG8H8_001644"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP8986715.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3222..5567
                     /locus_tag="ACG8H8_001645"
     CDS             3222..5567
                     /locus_tag="ACG8H8_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP8986716.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(5730..5996)
                     /locus_tag="ACG8H8_001646"
     CDS             complement(5730..5996)
                     /locus_tag="ACG8H8_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986717.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(5989..6312)
                     /locus_tag="ACG8H8_001647"
     CDS             complement(5989..6312)
                     /locus_tag="ACG8H8_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986718.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(6324..7580)
                     /locus_tag="ACG8H8_001648"
     CDS             complement(6324..7580)
                     /locus_tag="ACG8H8_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFP8986719.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(7577..7972)
                     /locus_tag="ACG8H8_001649"
     CDS             complement(7577..7972)
                     /locus_tag="ACG8H8_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986720.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            complement(8326..9327)
                     /gene="ccpA"
                     /locus_tag="ACG8H8_001650"
     CDS             complement(8326..9327)
                     /gene="ccpA"
                     /locus_tag="ACG8H8_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP8986721.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            9537..10640
                     /locus_tag="ACG8H8_001651"
     CDS             9537..10640
                     /locus_tag="ACG8H8_001651"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP8986722.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(10703..11317)
                     /locus_tag="ACG8H8_001652"
     CDS             complement(10703..11317)
                     /locus_tag="ACG8H8_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP8986723.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(11310..11759)
                     /locus_tag="ACG8H8_001653"
     CDS             complement(11310..11759)
                     /locus_tag="ACG8H8_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP8986724.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            complement(11884..12822)
                     /gene="galU"
                     /locus_tag="ACG8H8_001654"
     CDS             complement(11884..12822)
                     /gene="galU"
                     /locus_tag="ACG8H8_001654"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP8986725.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(12837..13862)
                     /locus_tag="ACG8H8_001655"
     CDS             complement(12837..13862)
                     /locus_tag="ACG8H8_001655"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP8986726.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(13906..14736)
                     /gene="lgt"
                     /locus_tag="ACG8H8_001656"
     CDS             complement(13906..14736)
                     /gene="lgt"
                     /locus_tag="ACG8H8_001656"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP8986727.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            complement(14751..15689)
                     /gene="hprK"
                     /locus_tag="ACG8H8_001657"
     CDS             complement(14751..15689)
                     /gene="hprK"
                     /locus_tag="ACG8H8_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP8986728.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         4450 a         3315 c         2866 g         5188 t
ORIGIN      
        1 caccgtttat tcggaaaaag gaactgcgac aagacttttg tcacagttcc ctttttattt
       61 ggaagataaa gcttttttca gattcaattt actaaaaact agcatgcttg ttactgcgat
      121 gaattgcgtt aatggcacgc ctgcgccagt gtagacactt aaaaaataat cctttttttc
      181 tggcgcataa gaacaaaacc acgagacacc gaaaagaaaa accaattgtg tcagtattaa
      241 tccgatgact acaatcaaag aggaactata ataggcacga tcagcttttg caaatacggt
      301 tcctagtatc ataagtaata tccctgctat tgaagcaatc agcacgggaa tggccagaac
      361 ttggtaaatt tttatcatga cattcgcgac ttttgctgtt tttgtcaact ggaaatcagg
      421 tgttctcgga tcactgctat cgatccaatt atccattagt ggaagattca ataattcttg
      481 tgcctgttcg acttgttctt gcgtcccaaa actataattc cccccttgat ggaaatcttt
      541 gtatcgccat gcataagcat atcctgattt cagaaaatca aatacgattg gcagatcatt
      601 catcgtcttt ccatcccctg atcccatcag atagaattct tttttctttt tgatagttcc
      661 cttttgatag gcttcttcta attcttgatt gacttttttc caaaaatcat tggtttcttt
      721 tgcattgttt tggtaataac cagattcgct taccacatct cgcaatgccc aaaaaatgat
      781 atctcctggt aattctttta cttttccgcc ttgggtccac gcatgagtcg tatacaattc
      841 gtcaatacga gtagaaacag tatgaaatgt aggagatgcg tctaaagctt tatttaatgc
      901 agcatgcgag acccatatct cactgttttc tttatttaag tttgtttcgt catctattcg
      961 gatcagctgt ttcatcaaac gtccaaattc gccactcgaa cggtcattga tggctgcaat
     1021 accatatgtt gtctcgtttt tatattcgat ccactgatag acgatggaga aaccgactag
     1081 agggagtaaa aacaatgaaa gtttcagcca ttcttgtttc gaaaggagaa atggtcgttc
     1141 ttttaacttc tttgcttctt tgaaagaaaa gtcttgtcct agcaagatct gaacgaaact
     1201 gatgcctaac gcgactacag taaaaggaag aagccagatg gaatcttcgc gaatgaacca
     1261 aaagaaagga aaagaaacag ctagccctaa aaaccacggc agcaattttt tgctggaatt
     1321 atatttgcgg taataaatag ctagtaaaca agcgataatg atctcaacag tcgggatcac
     1381 gagagcatta cgatatgttc gagtggaata ttcatgtgta aaactaatcg gagaaaacaa
     1441 aaaataaaaa tagatactta aaagaagata cttgttcttc acgattggtt taaacgctag
     1501 tgcaaccaat atactggcaa gaatactgaa catgccgaat aaaactgcat aagaaagatg
     1561 cagtttatgt gcaagtacta aaaacaagct gtaggaaatc ccttttgaaa gtgtcgtagt
     1621 cgtgtactcc cctagccatt taccatccaa aatattttga gcatagtgaa tcagcagctg
     1681 atcatcatag gtcgccgaca tatctgttcc atagattgct ttactatata aatacaaccg
     1741 aataattgta aataatacgg ctatgccaag aataaagtat gtatgtttct tttttagttg
     1801 gtcgatcaag gggtttaaaa tgttcagcat cttttcgtcc tttcaatcac gttttgctac
     1861 ttgtttgtga aacttttcgt tttttacttt tctatcagta taccctgttt gtctgaattt
     1921 aaaaagaggg ggcaaaagga gcatggcgaa aacgaatgca aaaagcactc ctttgtttct
     1981 taaacaaaaa aggacaaggc agactgccca atcctttttt gttgctgtca ggtagaaaac
     2041 ttaaagtgtg acttcttctg ctaataactc agggaacact tcgggatcga atgcttgcgc
     2101 tttgaacatg gaaatttctt ttccatatgg tgcgtaagct ttctttttat ctttaccgac
     2161 atatggggtt tctaatattt tcgaaatgtt gtcaaacatt ggatcatgga cgattttgtt
     2221 caatgtgtca aatccaattg ttccaaaacc gatattggca tgccgatctt tatgagaacc
     2281 ttgaggattt ttagaatcat tgatatgcag tactttaagc cgatctaatc cgatgacctt
     2341 atcaaactct tccattacac ctgaaaaatc ttcttttata ttatatccgg catcattcgt
     2401 atgacatgta tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg
     2461 cgctaattcc tcaaatgttc gaccgatttc cgtacctttc cctgccatcg tctccaaagc
     2521 gatctgtgcc gtctgttctt ttgtcaaaac ttcattcaac ccctcaataa ttctagcaat
     2581 cccagcttct gcccctgctc ctacatgagc gcctgggtga agggtgattt gagtagcacc
     2641 taaagcttcc gcacggacaa tctcttctct cagaaattgt acagcaaatg gaaacatttc
     2701 tggtttgatc gtatttccca aattaatgat ataaggcgca tgaacgacga tatttgttag
     2761 tccgttttct gccataaatg cttttccggc atcaatattc atctcttcga tagcttttct
     2821 tctagtattt tgaggagcgc ctgtatagat cataaaggtc gaagatccgt aactggctgc
     2881 ttcttctgcc gcgcctaaca gcattttctt tccgctcatg ctgacgtgtg agcctaataa
     2941 catggatgga ttcctccttg aaaggcaatt gtttcgaaca attcctgtat attgataaga
     3001 tgatgtacaa agaaaatagt ttgtttttct ttgccctgtt ctccatagta actgaaactt
     3061 tccctaaaga aaagcttaag aactctttca taaaaaagat catttcctca cgttttatct
     3121 cattcgaaaa agaaattcct gtcaaaaaca gccttgtctg tttttttgtc ctattatttg
     3181 ctataattag cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc
     3241 gaaaaaaaca gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag
     3301 tgtttcaatc attgactgtt tttattactg ttcttctttt gttgttcgca gctctaggta
     3361 tcggaatagg tgctggctac tttgcctatc ttgtcgaaga cacacaattg ccgactaaaa
     3421 aagaactgca gacagaacta ggaaacatta cggaaacgtc aaagattgtt tatgcagata
     3481 atacagagat atcaaaaatc cagacagatc tgatgcggac gactatttca tctgataaga
     3541 tttcgccttt attgaaaacc gcgattatca gtacagaaga tgaatacttc gataaacacc
     3601 aaggttatgt ccctaaagct gttttacgag cactagtttc agaagcgact ggcatcggct
     3661 cgtctggagg atccactttg acacagcaat tagtcaaaca acagatccta accgatgaaa
     3721 caacattcaa acgtaaagca aacgagatcg ttctttctgc acaagtagaa aaacactttt
     3781 caaaagatga gatcattact acgtatttga atgtctcacc atttggtcgg aataataagg
     3841 ggcaaaatat tgctggagtc caggaagctg ctagaggaat ctttggtgta gatgcaaagg
     3901 atgtgactct gccgcaagct gcttatatcg ctggtttgcc tcaaagccct atcacgtata
     3961 gtccatacac gaatacaggc gcgctaaaag aagatctttc tgccggtctt gccagaaaag
     4021 attttgtttt attcagtatg taccgtgaag gacaaattac gaaagaacaa tacgaagaag
     4081 cgaaagccta tgatcttaca aaagactttc tcccacaaca aatcgcggaa cagactgaca
     4141 gagaattctt gtattatacc gtgatgaacg aagcaacaag gattattgca caacaattgg
     4201 cagaaaaaga taatgctgat atgtcagatt catcggtcag tgaagcttat tatcaaaaag
     4261 cacagcaaac gatccaaaac aaaggatata cgatccattc gacgattgat aaagatatct
     4321 atgctgcatt gcaaaccggc gtagaaaatt atggctacat gttagatgat ggtgctggta
     4381 gccaagtgga gactggaaat gtattgatgg ataacagaac cggacgtatc tatggcttcg
     4441 tgggtggtcg gaattacagc cagaatcaaa ataaccacgc ctttgataca gaacgtcaag
     4501 ctggttcatc catcaaaccg gttcttgttt acggtcctgc aatcgatatg ggacttgtcg
     4561 gatcagaatc ccgtgtatca gattatgcaa cgacttggca agaaggaaaa aatgctggtg
     4621 agaaaatcgt caatgcgacc aatgaagggt cgaacacctt ccaaaccgtc cgtgagtcac
     4681 tggaatggtc caacaatatc ccagcttatc acttatatca agatgtgttg aattccggtg
     4741 gatcgaaaca atacgcttac gagcactact tggcgaagat gaactaccca gcaaatgata
     4801 attggggcgt tgaatcggct cctttaggga cagtagatgt aacgacgctt caacagacaa
     4861 atggattcca ggccttagca aatggtggag tctacgaaga aggctatatc attgattcca
     4921 tcacagataa tgcaggtaat gtgatttata aacatgaaag taattctgtc cgtatttatt
     4981 ccgaagcaac ggcatcgatc atgaatgaca tgatgcgtag tgtgatcaat gaaaaaatca
     5041 ctacaccatt caaagatgtc atctcaagct taaacggaaa tctaggaaat gcagattggg
     5101 tcggaaaaac tggttctacc aatgaatacc gagattcatg gctgatcgtc tcaactccat
     5161 ctatcactat cagtagttgg gcaggacacg atgacaatac tggaatggac agccaagcaa
     5221 gaatacgttc ggctaattat cttgccaatc taatcaatca agcttaccaa gcaaaccctg
     5281 agattttcgg cactgatgag aaatttgact tatcatccga tgtgatgaag aaaaaagtat
     5341 ctgggttcac aggacaaaca gctggaaaag ttactgtcaa taaaaaatct atccaaacgc
     5401 caggaaaaac agtggaatca ctttgggcaa aaaatggtcc gaagaaaagc gcctttaaat
     5461 tcggcgttgg cggtactgat gaaaactata cagattactg gaacaccgct tcagcttatg
     5521 ctagagcgaa tccagagacc aaagaatcag acgataaaga tgattgatct aatagattaa
     5581 tcagaattaa agggagctgc gacaaaaatc ttgtcacagc tccctttttc cgaataaatg
     5641 gtgttcaaaa gcttacatct acgatattaa gacaaattta tgtaaaaatg tgaaaaacca
     5701 ttttcacaaa atttttctat ataactttac taacgaaact gttttaattt cttttcttgt
     5761 ctttggaata attcggttag tttttctgca aattgccctt ttactgattc ttttaagtgt
     5821 gcatgtcggt tagaaaattc ttgagtagct tcttctgcag tcatttggtg gttttgatct
     5881 gtcaatcgtt tttctgccca agtttgctga gatatcccca taccaactga acgtacattt
     5941 gatcgatgta gatttttctc taattctttt agtaattgca ttaaaaattc attcataaga
     6001 tgatttctcc atagttattt gtctgatttt ttgacgatag gaacgttcta gttcgtcttg
     6061 ctgctgatag agttttcgtc gttctacttg tagggcttgt tgataatgct cctgataatt
     6121 cgaataactt cgtcttgctt gaaacagagg ctcattatct aaagctaagt tctttaatac
     6181 atgatgcagt cgctgctcct cttgctccat caatcgtaat ccttgattct taaagtggat
     6241 caattcatct tcttgagctt ctaattgtct tcgttcactt cgataatctc tttccaattc
     6301 ttctagtttc atctacttct cctttaattg aattgctgtg ctaattctcg atctcgtgcc
     6361 acgatttcat tgattttgtc ttggatctcc ttcgccaatg tatcaaaggc ctcccccata
     6421 cgtcttgcct tattgattcg tagcagatag acttctttcg cagaatcgac taagttctct
     6481 tttgtggcgc ccacactagc taatgcttct atgatttcct gttcagtcaa cgaaggggcg
     6541 acaacacgtg cttgccgcaa gccttcttgc cattttttct ctagctcaaa aattcctctt
     6601 tggttgatgc ggataacttc ttccatagcg cttttcattt ttaccgataa cttttccacc
     6661 actaacctag cttgtgtgtc atctaagtaa atttgttcat taactgtcag ccggccgcca
     6721 cttaatcgta gttttttacg tatgttgttc agttcttgca actgatagga aaacaactgg
     6781 gtcgcttggt tttctttctg ttgttttcga gctttaacat tgttttcatt ttcaggaata
     6841 atcagctttc cattttcatc aaacttccaa gaggttaaac tgtgggaagc aagcagattc
     6901 aagtatgctg tttccatact gatccgaact tcagcacctg tgccattacc accaaaatta
     6961 ccgataaaat cggcgtgatt tcggtaattg gtgagcatac cgggattttc ttccacccat
     7021 ttttttgctt ctggtgataa gagatcatac gggtccggcc cgttgaagcc gacatttttc
     7081 cattgtttct ctgctgctac atataatgct aagaatccac ctaatgaatg gccggtagta
     7141 gaaatagcag cttctccata ttcctttttt acttcattag caaatgcaat cgctgaattg
     7201 atttgccccg ctgttttcaa tccactaacg gataaaatct tgtttgttgt tgctttttca
     7261 gaaaaacttg ctcctggtct attcccatag agattcataa cacttcttgc atccgtttcc
     7321 aaatctttcc aatcgcttaa gttcgttcct gcaaaagcaa tcactacttg agaaatatcc
     7381 actttaccgt tcttgattgg cgctacagcc atcgcctgca tgccgtcgaa ggagtttttg
     7441 atttttagga tttggaattt tgtttctcca aagtttttaa tggcgccttc tttgtaactt
     7501 ttactatatt gaggatgttt tggatctagc caatatactt tattacttat tcctttatac
     7561 tgcttatcag taattgtcat actttttcct cactcccatc tgtatagata actataatct
     7621 catcaaccgt aatcccttct ttttgtactt cttcattttc aacaccatag ccatctatct
     7681 cattgctttc ttttgtaaag ctataatcaa aataaacggc ttttccttct gtattggtca
     7741 tcgttatcaa tgcttcatag attcctgtca tgtcgttttt ctcaaattgt tcgattttca
     7801 cttctttgat atccgcaaag gtatttttca gaactttgac ggactgtttt tctactgcga
     7861 gtaaatcttg ctgtttttgc gcttctcttt tgtccatgta aaattttcct ccgattccta
     7921 tagtaagaat ggtcaaaatg atgactatgg tctttcgtct tttcttaggc aaatagaagt
     7981 ctcctttcgg gttatgtatg atggattgtt tctcctttaa gataagtacg caatcgagcc
     8041 tggatttttt tggatttttt agagttactt ttctactttt ctactttctt ttttatcacg
     8101 ttttcatttt aaagtaattt tgatagtagt gaacaaaatg tagcatctac gcacagattg
     8161 tgtcgtatac gatggacgta aggtttgttt ccgccattct ctcttttttt taaagtcaca
     8221 aaaaaagggg ttatgacaaa attttgtcat aacccctttt ctcgtataaa ggttgttcca
     8281 gaagttgacc actttgggaa caacctttaa atgattgatc ttttattatt tagttgatcc
     8341 tttttcttcg attccataag gcaagataat cgttttttct tcgacttctt ctttattcat
     8401 cattttcgtc aataggcgca tggataccgc tcctaaatcg tataatggtt gtgtgatact
     8461 tgtcaaacga ggacgagcta cttctgtcag taaagaattg ttgcttgtga tgatttcgaa
     8521 ttcttctggt acttttacgc cgcggtctaa taaaccatcc aagataccaa tggctaattc
     8581 atcgtctgaa acatagacag ctgttgcacc gctattatgg atccggtcaa ctagagacag
     8641 accatcttta aagttgtatt gtgcctcaaa aatcaatcct tcgctatatg gtagattgtt
     8701 ttcgcttaat gcttctttat agccttttag acggttttga ccattgatag gatcaatcag
     8761 cgcaccgctg acaaaagcga tttttttatt gccgcttttt gctaatttat ttactgcatc
     8821 ttttgttgct gatacataat caatgtttac gctacctact tgttcatctg gatcaatgga
     8881 accagctagt acaacaggcg tttttgaacg tgaaaattct ccacggattt cgtctgtgat
     8941 atggtgaccc ataaagatca cgccgtccac ttgcttagcc aataaattat tcaatacatt
     9001 gacttctttt tgatcgttgc catctgagtt tgctaagatg atattgtatt tgtacattgt
     9061 tgccacatcg tcgataccac gtgctaaaga ggcaaagaac atattgctga catcaggaat
     9121 gattacccca actgttgttg ttttcttact tgccaagcca cgtgcgactg catttggacg
     9181 ataatccaag cgatcgataa cttccaagac tttttttctt gttgctggtt tgacgtttgg
     9241 attgccattt acgacacgtg aaacagtagc catcgaaaca tttgcctctc tcgctacatc
     9301 atagatagtg attgtttgct tttccatttg tgttctccta ttcatatttt atatgttttc
     9361 tgaaaatacg tcttacattt ctaaaatagg atatcaaata atctcaacaa ttgcaaccgt
     9421 tttatctctg ttttcatgaa aatttttact tttttcagtt ttaagaacgt tggtctttta
     9481 atcaatcagt ggtacaatta ttgaatacaa aattaattta gaaagtaggt tgccaaatga
     9541 ataacgtaaa aatcaatgaa ctacgtcaat ggatggcaca acaccaatta gaattggctt
     9601 atatcagcga ccctggccac atcgcttact tttccggata tgaaagtgat cctcacgagc
     9661 gtgtactagc tctttttatc cctctggaac aagatccttt ccttttcact cctgctctgg
     9721 aagttgaaga tgctcaaaac agcagctggg cgtacgatgt acgtggctac ttagacagcg
     9781 aaaatccttg ggagatcatc ggaaaagagc ttcgcgcacg ctatgggcaa cctggtaatg
     9841 taggtattga aaagaatgct ctgactgtcg atcgcttcga tgctatctct gatatcttga
     9901 caggtacaga ttcttacacc gatctaactt ctattatcca gcgtctgcaa ctgatcaaaa
     9961 cggtagaaga aaaagacaaa ctgatcgaag ctggtaaatg ggccgatgtt gcctttgaaa
    10021 ttggcttcaa aacagtaaaa gaaggcgtaa ctgaacaagc aattatcgca gaaatcgaat
    10081 accaattaaa aaaacaaggt gtttcacaaa tgtctttcga cacattagtc cttgcaggag
    10141 caaatgctgc aagccctcat ggtacaccag gaagcacgaa ggtttcacca aatgaactag
    10201 tgttattcga tcttggcgtt atttggaatg gctattgcag tgatgctact cgtacagttg
    10261 cttatcaaaa accaactgaa ttccaagaaa aaatctataa catcactctt gaagcacagc
    10321 ttgccgcaca agaggcagtt cgcccaggtg tgacagcagg tgaacttgat caaatcgcac
    10381 gaaacgtgat caacagctat ggctacggcg aatatttcaa tcaccgttta ggtcacggaa
    10441 tcggaacaac cgtccacgaa ttcccttctc ttgtagaagg aaacgactta gtgattgaag
    10501 aagggatgtg cttctctctt gaaccaggaa tctatattcc tgaaaaagta ggggtacgta
    10561 tcgaagattg tgtgtatgtg acaagtgatg gttgtgtgcc attcacgact acaccaaaag
    10621 aattacttgt tttagagtaa tgaaaataaa aaaggtgaga gctggtccgc tctcaccttt
    10681 tttattctag aaatgagtaa tcttagtgat tggtagtcga atctgtcatt gtatctttca
    10741 tttctttttt agcttctttt gcatcttctt ttgcatcttc ttttgccatt ttcaacattt
    10801 cttttgtgtc atctgcaact ggttttgctt ctgacattcc ttcattgacc gtctctcctg
    10861 ctttatctgc agcatctttt acatcgatca tgatatcttc tgaagtatct ttagcaatac
    10921 tacctaattc gtctgctggc tttttcagat cttctgctgt ttttttgaaa cgattagata
    10981 aatcagctga ttgttctttg aggtttaaca agatatcatc tgattcgctt cctgcttcct
    11041 ctgctgattc acttaccttc tgcttaacat tgcctgctag atcccctgct tgttgagaaa
    11101 gatcttgcgc tttttctttt gcaatacctg ttaactcaga tgatttttct tttgccatat
    11161 ctgtgtaatc tgaagccatg tccatcaaat catctgcttg agcagccaat tcatcccgaa
    11221 gttctttacc agatttgggt gctaacaata atgcggcgat tgctgcggct gtacctccaa
    11281 ctactgcacc taataaaaat ccaccttttt tagccattct tattttcctc ctgtcgtttc
    11341 agtatgtttt ttgtttttaa aaatttcatc gctgttccac cgacttttcc ggcaacagtc
    11401 gctttagctg tcgtttttcc gattccgccg actttcgtca cgatgttttt actagaatga
    11461 ttaagttccg atacactttc acttaaatca gcaactgctg tgaacaatgg atcgatcgtt
    11521 gcgacttttc cattgatatc caagagcagc tcattacttt tgactagcaa gccttctact
    11581 tgtctagata aaagatctac gtcttgtgtg acgatctcaa ttgtcgtatt tgcttctgcg
    11641 acagtctggt ctactttttc taaagttttg gaaacttgca acaatacgcg aacgatgaaa
    11701 acgacaagta tcgcaaaggc gattgctgcg atcaatcctg caatttctcc tcctgacatg
    11761 atcaaactcc cttcttgtct ctcttttcta taaggatagc attttcgtgt tttttaaaca
    11821 aataaaaacg agcttcaact actttttatt caattagcag ctgaagctcg ttagaaaaaa
    11881 agattatttt ttcttttctt ctttgatttc tgttattcct tttggattat gattgatctt
    11941 gcttccttct gcttctaatg ttttacctaa agacatgatg tattcacgca aaggtgcccc
    12001 tacttctgga tgtgtcagac catattcaat acttgttttc ataaaaccaa atttgtcacc
    12061 aacgtcataa cgtttacctg tgaatttacg ggcaaatacg cgttgtgttt tattcaacgt
    12121 atcgattgcg tcagttagtt ggatctcatt tccagcgcct ggttgttgtt ctgataatac
    12181 atcaaagatt tctggagtca ataagtaacg cccaataata gctaaatcac ttggtgcttt
    12241 gcttggatct ggtttttcaa caaagttttt gacattgtat aacccatctt ccagtacttc
    12301 ccctgggttg atgattccgt attttgaagt ttcatcgtga ggaacttgca taacggcaat
    12361 cgtagaagcg tgtgtacgct catagtcatc gatcaattgc tgtgtcaatg gtacttcgtc
    12421 ttccatgata tcatcgccta gcataacgac aaacggttca tttccaataa aggctcttgc
    12481 ttgaagaacg gcgtgtccta acccctttgg atgagattga cggatgaaat gcaaattgac
    12541 atctgtcgtt tcttctacta atttcaataa ttcggttttt cctttttcac ttaggtttgt
    12601 ttctaattcg atatttgaat caaagtgatc ttcgattggt cgtttagctt ttcctgtaac
    12661 gatcaagatg tcttcgattc ctgatttcaa cgcttcttca acgatgaatt ggatcgttgg
    12721 tttgtccacg attggcaaca tttcctttgc catcgccttt gttgctggta aaaatcttgt
    12781 tcctaatcct gctgctggaa taactgcttt ttttactttc atatacattc gctccttcaa
    12841 aaaattgtta atgagaattc attttctttt ttcccatcac gtaacatgat ttcttttgcg
    12901 gctgttttga tttctttttg ttcatacagc acttcataga ttgtttgagt gatgggcatt
    12961 tcgatttcca tcatttgggc tagttccatc gctgcttgag tagtattcac accttcaaca
    13021 accattccca tattggctag cacctcttct agaggctgac cttgtcctaa caagttgcct
    13081 gcgcgccagt ttctggaatg aacacttgtg caagtaacga ctagatcacc tactccgctt
    13141 aagccaataa aggtcagcgg gttggcaccc attgctacac ctaaacggct gatctcagct
    13201 aatccgcgcg tcatgattgc cgcttttgca ttatcgccaa accctaagcc gtgaatagca
    13261 ccagctccaa tggcaataat atttttcaaa gctgctccca tctctacacc aatcacatca
    13321 gggttggtat agattcgaaa ataatggttc atgaacagct cttgtacata ttttgctgct
    13381 tttacatttg tgcttgccgc tgtgatcgtc gtaatgtcat gctttgctac ttcttccgca
    13441 tggcttggcc cagaaagaac agcaacagct tttctttttt ctgtcgggat ttcctctaac
    13501 aagatttcag agattctttt atgcgttccc tgttctaatc ccttacttgc gtggatgatc
    13561 actggcttcg tattcaattt ggcaactagt tcttttgcaa cagaacgtat cgctttcgtc
    13621 gggataacga atagtaccgc atctgcatct tttactgcat cttccatatc ggtgaatgct
    13681 cggatggaat cgggtagatg atgatctggt aaataacgtt gattggtgtg ttgggtgttg
    13741 atttcttcta cttgtgaagc acgatgtccc caaatccgaa cgtcatgtcc gttttcagcg
    13801 aggacttgtg ctaaagcagt cccccaagag cctggtccta aaaccgctac tctataattc
    13861 attttaccac tcctgaaatt tttttcattt ttaacgctct gaaaatcaaa taatttgttg
    13921 attttttcct aatgagcggt cataatccgg taaacttcct ttttttctac gccaaataaa
    13981 cagaatcaat gtgccgacaa ataaaacagc agacaataat tgagaaatgc ggatcacacc
    14041 taaaaagtaa aggctgtctg tgcgtaaccc ttcgataaag aaacgtccga aactatacca
    14101 cattagataa cataaaagga cttctccttt tttcaaaaga tgtggtttct tccgcaaaac
    14161 caaaagcaga ataaatccaa tcaaactcca aagtgattca taaagaaagg ttggttgtcg
    14221 atagattcct tcgatataca tgttatcaat gatgaattgc ggtaaatgca gactttctaa
    14281 gaaacttcgt gtcgtgtctg gaccatacgc ttcgtggttc atgaaatttc cccatcgtcc
    14341 gattgcctgc gccaataata cgcttggtgc agcgatatct aaaaaggtcc acaaagaaat
    14401 aaaatgatat ttggtaaaaa agtacagtgt gattgcacct gcaatcaatc ccccgtaaat
    14461 cgctaatcct cctgaacgga tattgaatat ctggattgga tcagctatat attggggaag
    14521 atcgaataaa acataataca agcgggcacc aataatggaa atcggcaatc cccacaacat
    14581 aaaatctgtc acgtcatctg cttttaatcc tacccgaaca gcttctctgc tgctgagcca
    14641 catggcaata atgattcctg atacgataat caatgcatac cagtagatag gaattcctac
    14701 aagctcaaaa gcaactcgat tgatttgtgc taacatattg tcactccttg ctagtcattg
    14761 cctgagtttt cttcaatcaa gcgacttaat ctttcttcaa atgttttcgt tgcgtcaaag
    14821 cccattgtac gtgcgcggaa attcattgcg gctacttcaa tgatgattgc aacgtttcgt
    14881 cctgttttga cagggatttt tatttgtgga atgcccactt ccgcgatttc aacggtcgtg
    14941 tcttcagagc ccagacggtc gtatttctta tctttcgccc atgcttctag ataaacagct
    15001 aactggactt gcatagagcc tcgtaccgca ctcgcaccga aaagattcat tacatctatg
    15061 ataccgatcc ctcgaatctc aatcagatgt tccaggattt taggaggttc tccgattacc
    15121 gtcagttcat cttgctgata gacatctact cgatcgtctg cgatcaatcg atgtccgcgc
    15181 ttaatcagtt ccaacgccgt ttcacttttc cctatcccac tgtccccttg aattagtaca
    15241 cctaatccgt agacatcgac taacacaccg tggacacttg ttcgaggggc taagcgtcca
    15301 tctaaataac tagataattc acctagcagc cgggacgttg agataggcga acgcagcaca
    15361 gctatcccat tttcattggc tgcttggatc atttcttccg gagccggcaa tcctcttgaa
    15421 ataataaatg cgggcatatc ttcactacat aatcttctca taatgatcaa acgctcttct
    15481 gggatcatcc gttccgcaaa actgatttct ttactgccaa atagctgtaa acgattatgg
    15541 gaataataat taaaataacc ggtcaactct agtcctgggc gtgagatgtc cccagtcacg
    15601 atgacgcggt ctaaactttt ctcgtcgcct gtgacgattt ccaaagataa tttttttact
    15661 aattggctaa cttttacaac ttctgacatt catgctacct catttctatt agagtgttct
    15721 ctatcatttt atcattgtct agccaaaagt actaccaatt aaaaaaaacg tttttattta
    15781 attaccaatg taatagaaaa cgcattcttt attctaaac
//
LOCUS       DAZKAO010000064        15791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000064 DAZKAO010000000
VERSION     DAZKAO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.064"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG8H8_001658"
     CDS             complement(26..328)
                     /locus_tag="ACG8H8_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP8986729.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(548..914)
                     /gene="ssrA"
                     /locus_tag="ACG8H8_001659"
     tmRNA           complement(548..914)
                     /gene="ssrA"
                     /locus_tag="ACG8H8_001659"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1085..1858)
                     /locus_tag="ACG8H8_001660"
     CDS             complement(1085..1858)
                     /locus_tag="ACG8H8_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8986730.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMSNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2002..2352
                     /locus_tag="ACG8H8_001661"
     CDS             2002..2352
                     /locus_tag="ACG8H8_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP8986731.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2333..3049
                     /locus_tag="ACG8H8_001662"
     CDS             2333..3049
                     /locus_tag="ACG8H8_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP8986732.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3049..4497
                     /locus_tag="ACG8H8_001663"
     CDS             3049..4497
                     /locus_tag="ACG8H8_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8986733.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4567..5076)
                     /locus_tag="ACG8H8_001664"
     CDS             complement(4567..5076)
                     /locus_tag="ACG8H8_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986734.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5066..5791)
                     /locus_tag="ACG8H8_001665"
     CDS             complement(5066..5791)
                     /locus_tag="ACG8H8_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986735.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6014..8134)
                     /locus_tag="ACG8H8_001666"
     CDS             complement(6014..8134)
                     /locus_tag="ACG8H8_001666"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8986736.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8364..9542
                     /locus_tag="ACG8H8_001667"
     CDS             8364..9542
                     /locus_tag="ACG8H8_001667"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP8986737.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9558..10316
                     /gene="aroD"
                     /locus_tag="ACG8H8_001668"
     CDS             9558..10316
                     /gene="aroD"
                     /locus_tag="ACG8H8_001668"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP8986738.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10339..10824
                     /gene="ybaK"
                     /locus_tag="ACG8H8_001669"
     CDS             10339..10824
                     /gene="ybaK"
                     /locus_tag="ACG8H8_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP8986739.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10895..12148)
                     /locus_tag="ACG8H8_001670"
     CDS             complement(10895..12148)
                     /locus_tag="ACG8H8_001670"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8986740.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12265..13614
                     /gene="gdhA"
                     /locus_tag="ACG8H8_001671"
     CDS             12265..13614
                     /gene="gdhA"
                     /locus_tag="ACG8H8_001671"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP8986741.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13726..15072
                     /locus_tag="ACG8H8_001672"
     CDS             13726..15072
                     /locus_tag="ACG8H8_001672"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP8986742.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15380..15766)
                     /locus_tag="ACG8H8_001673"
     CDS             complement(15380..15766)
                     /locus_tag="ACG8H8_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8986743.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5163 a         2824 c         3174 g         4630 t
ORIGIN      
        1 ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
       61 acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat
      121 caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt
      181 tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt
      241 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
      301 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
      361 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
      421 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
      481 aataaaaaat gctcaaaccc ttataaaata aggatttaag catctagcga aatctgctga
      541 aatctcacaa tggagacggc gggagtcgaa cccgcgtcca aacacatcgc cacttaaaaa
      601 tctacgttca tagtcaactc atttaaggat tcactttaca gcttgccgag tgacaggcat
      661 tctgtatcgc tagtctgatg ttctcttttt caacttacag acggaaagtt gaaacgtatc
      721 ccactaaagt taggaccctt actcgagcac atgggcgatg ccgagaggat ctacgctaac
      781 tgtttttagg cagctaaagc gtaagagttg ttttcgtttt tagcagttat atttaactgt
      841 aacgttttta cgtagacgta acctacgaaa cgcaattcaa gctcgactgt gcctgtcgaa
      901 tccgtaacgt ccccgttagc taacaagggt tacgtgtatt tttcatacaa aattagtata
      961 gcatatattg ggataaattc aataaaaaag cgaatcatat cccattattt atataataac
     1021 tgcccctttc ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa
     1081 gatcttaaat cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct
     1141 ttgtgataaa ggtagaaagt tcttttaatc cgtcataggc gttgctcata gcgattttgt
     1201 tgtcgcaagc ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa
     1261 ctttttccag ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt
     1321 tcttcactgt atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt
     1381 cgtgatagta ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt
     1441 taaagcctaa aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca
     1501 ttgtttggct atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac
     1561 cgttcaactg tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat
     1621 agatttcttc gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc
     1681 ccgcatcttc catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga
     1741 gaacattgtt ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt
     1801 tcgcatctag taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa
     1861 ataaaactcc ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac
     1921 ctttcgtgaa gaattcctga acatattggt tcagtcaaca atttagagta tactataaga
     1981 agagaattta ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt
     2041 ggcagtcttt ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg
     2101 cctcttgccg caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg
     2161 ttttttctta tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat
     2221 acgaattatt ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt
     2281 atgacagaaa agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat
     2341 tttaatgatt gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga
     2401 aggttgggaa gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa
     2461 cccacaacat tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct
     2521 agtaggaaga gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag
     2581 agattctgaa agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa
     2641 acctttcagt cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt
     2701 ggcagaagca agagaagaag cagtagtttc agatgatcat tttgatatcg agactagtca
     2761 tttcaagatg aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact
     2821 gacgcctaaa gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc
     2881 tcgggagcag ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt
     2941 ggacgcccat atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca
     3001 aactgtttgg ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg
     3061 tatcagcaac tcttagcttt ctgcggaatg gtcgcgttga tcttattgat cgttggagtt
     3121 tcttttacac aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga
     3181 tatgctgaat cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt
     3241 caagatcagg cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat
     3301 gtgaacttta tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta
     3361 ctgaatttta ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa
     3421 ttcacatcga ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca
     3481 tttaatttga atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc
     3541 gataacagcg ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc
     3601 gttgcagtta ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta
     3661 aggaatgcga cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac
     3721 aaagatgaat ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa
     3781 tctcaagcag aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct
     3841 cacgagatga gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat
     3901 gcaatccctg aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt
     3961 ctgatccgtt tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt
     4021 atggtcatta aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa
     4081 gctaaagcag aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt
     4141 tacgctgatt atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag
     4201 tttactgaaa acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc
     4261 acgattgaag atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt
     4321 tattataaag tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta
     4381 ccaatcgtgc agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta
     4441 ggaaaaggaa caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa
     4501 acaagcggta taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg
     4561 attgatttag cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc
     4621 atgagtaaag agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag
     4681 tagctcttgg atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt
     4741 tcgcttggaa agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc
     4801 agctgccgca caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg
     4861 atcttgtttg atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata
     4921 aggaaacatc ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac
     4981 ctcttttttc aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt
     5041 caagtgttca gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca
     5101 aatgatggat aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc
     5161 caataagagt ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat
     5221 cgatggtaaa aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga
     5281 tatcacggat atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa
     5341 gcgcttcata aactgactgg aagcttacga tccacaataa aaccaagata caacaaagaa
     5401 agagaggaaa gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag
     5461 tcaggtgcca attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga
     5521 taaaaaggaa ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag
     5581 ctaattctaa agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat
     5641 cttggacttg attggctaaa gccgattgca aatggtaaag agtcaggcat gctatcaata
     5701 aaaaagcaga aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta
     5761 atcgagcagt agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa
     5821 atgaatatga ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata
     5881 ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc
     5941 ttttgaacac tgtttattcg aaaagaggag cagcgacaat aattttgtcg ctgctccttt
     6001 taaatttatt ttatcattcg gaagaacttg tggtcgttcc gtgaagttct tggtacgttt
     6061 gcgttttata aagatcaaca gttgattgtc cgttgtgttc actaaagaca ctagttgact
     6121 tgtcaccaag agatttttca gcttctttta atttctctaa gccattcttg tagtcataat
     6181 cactggtatc aattgggtct aatccactat tcgtatagaa ccgaagaaga tcaccattat
     6241 tgatttggtc agaaacagct aactgtttat caactttttc tttcagtgaa tcaatttcat
     6301 tctgtacttc ctcagtaggt tcagtaatag cttcaccagt ttcgttacta tagatcgtac
     6361 caccgtagta agtatatttt ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt
     6421 catgatcttt cgagaataga tcttgtccta actgaatata attttttgta tctacaccta
     6481 gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc
     6541 cctgtgtttg tccagggata tggatcatat aagggacacg ctgcatttgt gcattgtcgt
     6601 agtttgtcca agtagaactt gtttttccaa ctaattcagc tagattttta tttcttgaat
     6661 tcgagacacc gtaatggtca ccgtaaagga cgatgactga attatcatac aagccgcttt
     6721 ctttcaaata attgaagaat tcttgtacgg cggtatctaa ataattggct gtcgcaaagt
     6781 aaccgttgat cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg tcattggtga
     6841 attttgaata aggataatgg ttagatacag caatgaattt tgaataaaat ggttgttgca
     6901 gacgttccaa gtattgtacg gattgttgga aaaatggttt atcatgcaac ccgtattgga
     6961 acgaattgtc atcattaaca tcataataag aagaatcaaa gaaataatca tatcctaaat
     7021 tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt cccatggaat accgcactaa
     7081 tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg gaaagtattt tttccgccta
     7141 attgtgtgaa caaggagcct tgattcaagc caaacaatga attttccatc agcgtttccg
     7201 catcgcttgt ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt
     7261 tgctgtggta aagactattg atgaaaggtg taacttcatg ctggagacca ttctcatctg
     7321 tcaatttata atcgatcagg aattgctgga agctttctaa atggatataa atcacatttt
     7381 ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc ataatgttct tttacataat
     7441 tttctacttc tttcatatca ttgggacttg cttctgcccg cacttggttt gtctggtaag
     7501 tttgaacacc gtcataaacc atgaaagcat tcaagccaag atatttgacc aaataatcac
     7561 gagagaatgt tcgagtcagc aattctggac gatcagcttc cgccataaaa agatttcctg
     7621 agaaaatcat aactgctaat gtagagatcg caaaagccat tcttgctcgg atcgggcgag
     7681 ggtccaactt gatttttttt gcaatcagca gaacagtcaa gataataaaa tcgatccaat
     7741 agatcacatc atatgggcga aacagtcgaa tggcactttc tcccagacca ctcgctactt
     7801 tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga taataagtga
     7861 cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac catcatcgtg atataagcaa
     7921 ctctagggcg tctgatataa agagcaattc caagtaataa caatgtagtt gcaatagggt
     7981 tgataaacaa gataaagtat tgaatgccac tttcgattcc gagatggaaa tcaactaaat
     8041 aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct
     8101 tattcaaaaa ggcaggtgtt tttatacatt tcaagataat cttcctttct gcacttctta
     8161 atcacagaag tgtcctgaaa ccatgttaca cagctcactt attctcattt attttactag
     8221 tatgacaata tagtgtcaat tgaaaccaga agtttatgct gtttcattaa ggttttcttt
     8281 aagcttcatg aaaaaatgag cagtaagaca agtcctatgt tttttgttat acttgggaag
     8341 agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat
     8401 tgaaaaaagg atatccactg atccaaaaag aagaccttgc tcatccccaa aaagaaacca
     8461 cgcaatgggt ggaattagtt gattcgcaaa accaatttat cgcaaaagga tatctaggaa
     8521 agcagaataa gggaatcggt tgggtattga gccagcagaa catcccattt gatcaatctt
     8581 tttttgaaga attttttgtg aagtcaaaag aaaaaagaac tttctttttt tcagatgatt
     8641 ctactaccgc ttttcgtata ttcaatggag aaggagacgg gatcggcggt ctgattgttg
     8701 attattatgc aggttatgct gttttttctt ggtataacga aacgttatat gcacatcgtg
     8761 aattattgat tcaagctttt cgtgaaatct acccggaaat cataggaggc tatgagaaaa
     8821 ttcgatttga atcagtaaat ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg
     8881 aacccttgat cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat gaaggattaa
     8941 tgactgggat ctttttagac caaaaagaag tacggggatc attagcagaa ggtttcgctt
     9001 ctgggaaaac agttttgaac atgtttagtt ataccggcgc cttttctgtt gctgccgcaa
     9061 tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa acgcagttta aaaaaaacac
     9121 aggaacagtt tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt gttatggacg
     9181 tattcgagta tttcaaatac gccaaaagaa aacaattttt atatgacatg attgtattgg
     9241 atccaccaag ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa gattatgggg
     9301 aactggtcaa ggatagtctg gatattttag cagaagacgg gatacttatt gcgtctacaa
     9361 atgctgcgaa tgtctcgata gaacaattcc aaacaatgat tgaagaggaa ttccatgcca
     9421 aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc aaaagatttc cagacgacgg
     9481 ataccttttt agaaggcaat tatctgaaag tgtttgttta tcaaattaaa aataaccatt
     9541 agaaggagcg atgcagaatg gttcgggtaa agggattgaa tataggaagc ggtagaccaa
     9601 agatttgtgc gcctcttatc ggggccgtcc gagaggaagt attacgtgaa gcagttcttg
     9661 cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt
     9721 cttttgatga agtagcagaa acattggttc ttatccatga gatgcttgat ggactgccgt
     9781 tattatttac gtttcgtacc ttagctgaag gcggagaaca agacatatcg atccgtcagt
     9841 atcgcgacct ttatgaacat gtgatacaca caggattagt agatatggtc gatatcgagt
     9901 tgttcaaagt agaaagtcta ggaaaaagac tgatagagga aatcaagtct ttaaacattc
     9961 ctttaattat cagtagtcat gattttaaag aaacaccagc agaccctgtc ttgctttacc
    10021 gattgaatgt tatggaacat tttggagccg atatcggaaa actggcagta actcccaata
    10081 atgaacgaga tgttttgcgg ttgatggagt tgactagacg ggcaaatgct tttgtctcca
    10141 tgcctattat tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa
    10201 cgacgggttc cgttatgaca tttggttcct taagcagcga ttctacttct gctccaggac
    10261 aattaccggt caaagcattg aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca
    10321 agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg
    10381 gtcgaacaaa aaaagattcc ctatacagag cacgaatatg agtgggacga aagccaccta
    10441 agtgcgtcat cggtcgctga acagcttcca gaaagccaat ctcgtatatt taaaacactg
    10501 gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga taccgggaga agccgaattg
    10561 aatctaaaaa aattagcaaa agtttcaggg aataaaaaag tagaaatgct ccatttaaaa
    10621 gatttggaag caacgaccgg atacatacgt ggtggctgct cgcctatagg tatgaaaaaa
    10681 ctatttccta cttatttaga tcaaattgca gaaagctatg aacaaatcat tgtctcggct
    10741 ggcagaagag ggctacagat ggagttagca cctcaagata tctgtgcatt aacgagcgga
    10801 caatttgcag atataaaaca ataaatcata cgtataaaag aaagaaggag gccgggatgt
    10861 tgtttgtcca gtctccttct ttctttttta tacattatgc tctttgcccg gcattcattc
    10921 ctgctacatg ccctgtagcg aaagccgctg tgatattaaa gccgccagta taaccattga
    10981 catccagtaa ttctcccgca aaatacagac catcaatgat tttactctcc aatgttttag
    11041 gataaatttc ctttagagaa acccctccgc cagttacaaa tgatttctca ataggaaaag
    11101 ttttgttgat tgatagctta aactctttgc atagttgaac aaaatcttga atttgttttt
    11161 gagaggcttg ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag aaaactaaca
    11221 atcgctcagg caatatacca tgccaagcat tgaccaagtt ctttttggtt gctttacttt
    11281 tttctgtcaa tatatgaata agctcttgtt tggtttgtgt agggaaacaa tccagagaaa
    11341 gtgtcactgg ttctcccgtt ttctctaatt ctttattgac aaaacttgaa caacgcagtg
    11401 cagccggacc agaaataccg aaatgggtga aaagtaaatc catcgtatgt tcagtcaata
    11461 cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg
    11521 ttttctcctg aatatacggt tcatccgata tcaaggggga ctctgtcgca taaagaggcg
    11581 tcaccgtgtg acccactcgc ttcacgatct tgtacccatc tccagtagaa ccagtagatg
    11641 gataagtccg tccaccagtt gttaagataa tggcaggagc atggaactct tcgaattccg
    11701 tgcgtatacc gtaaatttta tgttctttgt ggatcaattt ctctactcga gtagagaata
    11761 gaagcgtcac atctagttct ttcagacgat tgagcaatgc ttcgataatc gtttttgatt
    11821 tatttgttac aggaaacatc cgtccatgat cctcttcttt taaatgtacg ccttgtgatt
    11881 caaagaagtt catgatatcg aaattattcc attgcgcaaa cgtactatat aaaaactttc
    11941 catttccagg aatatgtgca atcaaatcat caacaggacg attgttcgta acattgcacc
    12001 gaccaccacc tgtcatcagt agtttttttc ctgctttttt gtttttttcg atcaagagta
    12061 cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat cccgcttgtt ccagcgccga
    12121 ctacgatcac atcatacagt ttttccattc cctcacctca aggaaagtgt agcatatctg
    12181 aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga
    12241 agataagcaa aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa
    12301 gaaaagctac atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc
    12361 atgcccacgg ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta
    12421 gaacttttaa cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa
    12481 ggaaactggc gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat
    12541 aaaggtggat tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt
    12601 gaacagattt tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt
    12661 gacttcgatc ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg
    12721 ctggaattgt caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta
    12781 ggagctagag aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact
    12841 ggtgtgttga caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact
    12901 ggttatggat tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat
    12961 caaaaaacag tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg
    13021 caagagctag gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca
    13081 gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg
    13141 gaatacgcaa accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg
    13201 aaagaaaaag cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg
    13261 gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt
    13321 acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct
    13381 gctaatgctg gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg
    13441 acttgggaaa gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat
    13501 acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat
    13561 gttgctggat ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg
    13621 aaactttctt ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa
    13681 acgtaaagta ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat
    13741 tttgactatt caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc
    13801 caagtaacag cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc
    13861 ggctggatcg atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca
    13921 gctaaaaaaa tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat
    13981 ttaggcgctc gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc
    14041 aacacaagaa aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt
    14101 gctgacttga tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct
    14161 ggaacaacta ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa
    14221 tatggacaag aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca
    14281 gttaaagtag aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc
    14341 ggacgtttct ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat
    14401 atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta
    14461 gaagaaaacg aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa
    14521 gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa
    14581 caattgtatg gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac
    14641 ttctcaactg atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt
    14701 gaaacagttg tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa
    14761 gatttagacg gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca
    14821 tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt
    14881 ccagaaatgg atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt
    14941 atttcaggtt acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag
    15001 aaaaacatgt tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac
    15061 gaacgcctgt aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt
    15121 cgctttaaaa tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca
    15181 gaataagggg ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga
    15241 ttgccttgct ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt
    15301 aattctgttt ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt
    15361 tttgcgatat attcttagct taagataggc ctaatttttc ttttacagat gagggtacct
    15421 tatcgctggt aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga
    15481 tcgttccttc ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat
    15541 ctaactgtct tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc
    15601 caattacttc cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta
    15661 ccgcataata atctttacct acataagtag actgatagta gtcccaacct ttccatccta
    15721 taaaagcaat caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt
    15781 tttattttta t
//
LOCUS       DAZKAO010000065        14728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000065 DAZKAO010000000
VERSION     DAZKAO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.065"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..777)
                     /locus_tag="ACG8H8_001674"
     CDS             complement(211..777)
                     /locus_tag="ACG8H8_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8986744.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(777..1733)
                     /locus_tag="ACG8H8_001675"
     CDS             complement(777..1733)
                     /locus_tag="ACG8H8_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP8986745.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1733..2389)
                     /locus_tag="ACG8H8_001676"
     CDS             complement(1733..2389)
                     /locus_tag="ACG8H8_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP8986746.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2759..4777
                     /locus_tag="ACG8H8_001677"
     CDS             2759..4777
                     /locus_tag="ACG8H8_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8986747.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4847..5053
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5204..7618
                     /gene="leuS"
                     /locus_tag="ACG8H8_001678"
     CDS             5204..7618
                     /gene="leuS"
                     /locus_tag="ACG8H8_001678"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP8986748.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7966..9519
                     /locus_tag="ACG8H8_001679"
     CDS             7966..9519
                     /locus_tag="ACG8H8_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP8986749.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9900..11684)
                     /locus_tag="ACG8H8_001680"
     CDS             complement(9900..11684)
                     /locus_tag="ACG8H8_001680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986750.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11699..13420)
                     /locus_tag="ACG8H8_001681"
     CDS             complement(11699..13420)
                     /locus_tag="ACG8H8_001681"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986751.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13520..14281
                     /locus_tag="ACG8H8_001682"
     CDS             13520..14281
                     /locus_tag="ACG8H8_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8986752.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4791 a         2715 c         2931 g         4291 t
ORIGIN      
        1 gaataaagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa
       61 cagcttctca tgtttttgat ttggttggac ttattaccgc agatataagc ttttgagcac
      121 cgtgaataaa gaggttatga aatcaacgct ctaacctgag tattagagaa aaaaacgcaa
      181 aacatatcat ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat
      241 aggcggctgg ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg
      301 cagtttggaa caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata
      361 gtagacgaca aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc
      421 tgtgcgtgta gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag
      481 attgaaaatc cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg
      541 gccttgtaga aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt
      601 agcatcgatt gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg
      661 ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc
      721 tggttgcaag atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag
      781 acgctcacct ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct
      841 tcatcaattg cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa
      901 tcccacggcg catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc
      961 tggtcgcaaa tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga
     1021 tagtcccgca acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg
     1081 atatctgaat ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct
     1141 ggtaatccgt tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg
     1201 ccatctaaat aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa
     1261 gttgtttgca atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa
     1321 tcaactactt catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct
     1381 ggtaaattga ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt
     1441 gtaaaatttt ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg
     1501 tcgatttctt tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc
     1561 gaaccagata cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga
     1621 cagtcaaatc ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa
     1681 gcgtaattcc aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt
     1741 ccttctttct gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca
     1801 ggcacagcca agaaagactg agtaagtagc cgccgatgat atcagtagga aaatgaacgc
     1861 ccagataaat gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca
     1921 atgtgcgtaa ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa
     1981 gaatcatact gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt
     2041 gcgccaatga aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag
     2101 caattacaat aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca
     2161 tccataaagc cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca
     2221 taaagagttg gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact
     2281 gatcgaaacc agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca
     2341 ggacaagaaa gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc
     2401 tttgaagcaa tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa
     2461 aaccaatgta tgattcctct ttgaataacc aatattctta taaaaaaaat aactgtaatc
     2521 ggaaagacat tttttggtaa tgcaaatgaa atgttaggga aataaaatca gtgtttttcg
     2581 aaatcctttt aaacaagaac gtagacgtag gaagaacttg aaaatgaaca gaagttgttc
     2641 attacggtaa attacaatat gattacaaaa aacaacaaag ctcttaaata acttttaata
     2701 aatattaaga gaagttataa ttattaataa tagataaatt gaagttggag gatagaagat
     2761 ggaaaacatt gcaagaaaag aacggagacg tttagaacag accaaacgtt ttcgaaaagc
     2821 aaaacgaggg gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg ctgccccatt
     2881 agtacagccg gttcctgttc aagcggatga aacacctgtt cagttcggcg caagaatcaa
     2941 tacagcggca tttatcacag aaattgctac ttatgctcaa ccaatcgctc aagccaacga
     3001 tctatatgca tcagtgatga tcgcacaagc agttgtagaa agcggttggg gcagtagtgc
     3061 gttatctcaa gcaccttatt ataatctttt tgggataaaa ggcagctatc aaggacaaac
     3121 cgtctacatg gatacgcttg aatttttaaa taatcaatgg gtaaataaaa aggagccttt
     3181 ccgacaatat ccttcttttg ctgaatcttt caatgacaat gcctatgtac ttagaaatac
     3241 ttcatttggc aacggttatt actatgccgg tacttggaag agcaacacga aatcctatac
     3301 agatgcgaca gcttgtttga cagggcgtta tgcgactgat cctggttacg caggcaaatt
     3361 aaataatatc atcgcaactt acggattgac ccaatatgat acaccagcta gcggaaacgc
     3421 aggcggtgga gcgactacag gtggcaataa tactggaagt gcaggtagta ctggcggttc
     3481 aagcaattct ggtaatacaa actcaggttc agcaacttcg accacttata ctgtccaatc
     3541 tggtgattct gtttggggaa tctcaaataa attcggtatc acaatggatc aattgatcca
     3601 atggaacaac atccaaaaca atttcattta tccaggacaa aaactgacaa tcaaaggtag
     3661 ccaggaaaat ggctcttcga cgaataattc cggtaacaat accaactctt caggaaatgc
     3721 tggtacatca aacggaggac aaacaactgg tgcgaaatac actgttcaat ctggtgattc
     3781 tgtttggaag atctcaaatg atcacggaat tacgatggct caattgattg aatggaacaa
     3841 tatcaagaac aatttcgttt atccaggaca acagctaatc gtatcaaatg gcggttcttc
     3901 taatgctgga gcaaatactg gctcaaatac tgggaataca agtggctcta gttcgtccag
     3961 ctcaagttct tctaatacag cttcaggaac gaaatataca gttaaagccg gagactctgt
     4021 ttggagcgtc tcaaataaat acggaattac gatggctcag ctaatccaat ggaacaatat
     4081 ccaaaataac tttatctacc caggacaaca actgactgtt tcaaacagtg gatcaacaaa
     4141 cagcacgtca ggtaatacag gttcgtcaac caatacaaat aacagcagtt cagttaatac
     4201 tggcagcagt tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg tttggagcgt
     4261 ggcaaataaa aacggcatca cgatggatca gttaatcgaa tggaataata tcaaaaacaa
     4321 cttcatttat ccaggacagc aattgatcgt taaaggtgga acttcggcaa atacaaatac
     4381 aggttcaact acttctgcag cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac
     4441 atcatccact ggtaatacga tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc
     4501 gaacaaacat catatcacga tggatcagtt aatcgaatgg aataatatca aaaataattt
     4561 catctatcca ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca
     4621 atcagttact ggttcgaaaa cctatactgt gaaatctgga gaatctgttt ggagtgttgc
     4681 tgacagtcat ggaattacaa tggctcaact aatcgaatgg aacaatatta agaacaactt
     4741 tatttatcca ggtcaaacgt tgattgtagc taaataaaat agctgttcga agaaataacg
     4801 attgaattcc acaaaaatat ttgttactat aaggctactt aaatatgaca acacttgaaa
     4861 aggaagtagt aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga
     4921 tgcttttgaa ctcgcctttg agttgccctt gctgaatacc cgagtaagca agcggcggtg
     4981 acggccgtta caacgttcga ggccttttca ctaaggtaaa attaggtggt accgcgtaga
     5041 ttacgtccta taaaaagagc aatcttttta taggattttt ttatttcttt tgtgaagttt
     5101 gagaaaacaa agcaaaagga aataaaaata aacagattct gccggttttt tcgttatatt
     5161 tataatagaa acaagtgttt tcaatagaac aggaggacag caaatgagtt acaatcataa
     5221 agaaattgag aaaaaatggc aaaaatactg ggcaaaaacg aatactttca acacccatga
     5281 tgatcctgaa aagccaaaat tttacgcact ggatatgttt ccctatccat ccggtcaagg
     5341 gctacatgta gggcatcctg aaggttatac ggcaacggat atcctttcac gtgtgaaaag
     5401 aagtcaagga tacaacgtgc tgcatccaat ggggtgggat gcattcggtc tgcctgctga
     5461 acaatatgcg ctagatacag gaaacgatcc agctgagttt acccaaaaaa atatcgaaac
     5521 attccgacgc caaatcaatt ctttaggatt tagttatgac tggaatcgcg aagtgaatac
     5581 aactgatccg gagtattata aatggacaca atggatcttt acgaagcttt atgaaaaagg
     5641 tctagcttat gaagcagaag tagcggtaaa ctgggtacct gaattaggaa cagttatttc
     5701 aaatgaagaa gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc
     5761 aatgcgccaa tggatgctga aaatcactgc ttatgctgat cgcttattgg atgatctaga
     5821 attagttgat tggccagaaa gcattaaaga aatgcaaaga aattggatcg gtcgttcagt
     5881 aggagcaaat attgaattta aagtggctgg tacggataaa tcctataccg tatttacgac
     5941 acgaccagat actctattcg gtgcaactta ctcggttctt gcacctgaac tagatcttgt
     6001 tagagaaatt acgacccctg aacaaaaagc tgctgtagaa gcttatatcg aagagacagc
     6061 taaaaaatca gatctaaaga gaacggatct tgcaaaagaa aaaacaggag tatttactgg
     6121 tgcttatgca atcaatccag ttaatggcaa ggaaatccca atctggatcg ccgattatgt
     6181 attatcttca tacggcacag gagccatcat ggctgttcca gcacatgatg aacgtgactt
     6241 tgaattcgct caaactttcg gactagaaat tcttccagtt atcgaaggtg gagatgttga
     6301 aaaagctgct tacacagaag atggtacgca catcaactct gaatttctag acggcatgaa
     6361 caaacaagaa gcaatcgata aaatgaatga atggctagaa gaaaatggcg tagggaaaaa
     6421 agaagtcagc tatcgcttac gtgactggct attctcccgt caacgttact ggggtgaacc
     6481 aatcccaatc atccattggg aggacggaac agtcacagct gtcccagaag aagagctacc
     6541 attacggttg cctaagacaa aaaacatcaa gccaagcggt actggagaat ctccattagc
     6601 aaatatcgag gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga
     6661 aacgaataca atgccacaat gggcaggaag ctcatggtat tacctgcgtt atatcgaccc
     6721 tcataacaaa aaagaactag cagattatga aaagttgaag cgttggctac cagttgacat
     6781 ctacattggt ggagcagaac atgcggtact tcatttacta tatgcacgat tctggcataa
     6841 attcttatac gatatcggtg ttgttcctac aaaagaacca ttccaaaaac tatacaatca
     6901 aggaatgatc ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa
     6961 tcctgatgat gtggtcgaaa cttacggtgc agatacactg cgcatgtacg aaatgttcat
     7021 ggggccatta gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt
     7081 cttggatcgt gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg accgtatcac
     7141 tacattgaat gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga
     7201 agactatgaa aatcttcatt tcaatacagc aatctcccaa ttgatggtct ttatcaatga
     7261 agcctataaa gtggacgctc tgccttatga atatatcgaa ggatttgttc aactactcgc
     7321 accgattgct cctcacatgg gtgaagaact atgggcaatc cttggaaatg atggtggtat
     7381 ttcttacgca ccatggccaa catacgatga agcagcactt gttgaagacg aagtagaagt
     7441 tgttttccaa gtaaacggta aagttcgtgc gaaaagcaat gttccccgag acttaggaaa
     7501 agatgagcta gaaaaagcag cattagccaa tgaaatagtg caagaatata ttgaaggcaa
     7561 aacagtcaga aaagttattg cggtaccaaa caagttagtc aatatcgttg ctaattaatt
     7621 ggctctttgt caataaggac tgatgagttg tgcaaaatca aatctgggcc agaatgaact
     7681 tcattctgtc tcagattttt tgttatttca aattgattaa ttgattgatc aaaaaggttt
     7741 tgattttcat gttctcaaac acgttcaatg tctttttctt ctgaatggtc ttgtaagaga
     7801 tctctgtaat catcaaattt ttgtctatta atcttagtat tttttgatgt ataaactacc
     7861 tgaaattgag agtacatacc aaattcataa attttttatt agtgggacta gtgattgtat
     7921 ggattttgac gcataatgaa tgaagtatga gaaaaggaga gacaaatgga tcaagaacta
     7981 tttattatcg aagtattatc agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg
     8041 aaaagaataa tgttcaagct tttaagctta gtatttttct ctggtttgct ttttttcgga
     8101 gctgtatcgt tcctatatcg ccatatgagt gcagtagtcc gttcagctga agggatgcaa
     8161 aaattaaata atttgtatca cttttcgtgg ggagttggcg gaataacaat tgttgtgctt
     8221 attgtttttc ttctacttat ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa
     8281 tggcttagtc aaagagcaca acagttttta cataataaac caacttctca agatgatgac
     8341 tatctttatt ttgagaagac ttcgacgagt caagcttttc ggatacaaaa aagtgattgc
     8401 cagttattgc tgagatcact tgatgattta cctatctact taggcgaaag caaatcaaaa
     8461 ttcggaatga acacattcca tattttttta ctagaaaacg aacccagatg ggaaaaaagc
     8521 cagtcttcgc ctggtggata cctgacaact aagatgagta cattagtcac tggttttttc
     8581 ctcattcttt tagctggaac aatctattat gggagataca ctcagccaaa aatgtcttct
     8641 catataaatg ctgcttctca catgacaatg aactcaacta acgatacgca tatacaaaag
     8701 acaaatgaag ggttgttagc tgatcctaag caaaaggtaa atttgacaac acaaaagata
     8761 aatgatctca aacttgatac agagacgcac tctctctata tgacaacaga tagtggtgct
     8821 aattgggtat ttgtgccgat tgattcgcaa tggttgcgtt ttggcgaata caccctgaca
     8881 acggggctga ttcctgaagg ttattggatg gacaacacat atgatatttc cgccgatttc
     8941 tcatggttta tctattcttc tggggatgat gtacgtctgc tgacttcaag agataatggt
     9001 aaaacatggc aaacgtctat tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg
     9061 gtattttatg gcaacgatgg tctatttatc tgtacatcag ccagtgggat gtctgcagaa
     9121 actttgtaca tttacactac tatggatggc ggaatcactt ggataaaaag tggaagtaca
     9181 atgatcaatc agccagtaca aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg
     9241 acacgtgaaa gtttgtttta tacaaacaac ggaggacgct cattcaaaga atcagttctg
     9301 tcgattccgg aaaactatca gacagggggg atagatcttt ttcaatcacc aaatgaagtt
     9361 actcaaataa gcgcgaatac gttagagaca aaattttatt tgttgaaaaa aggaccaatc
     9421 gatgcaggga aaatgtttgc ctgcctcttc cgttcaactg ataacgggga aacatggcaa
     9481 ttcgttcagc aactatcgca agtagaacaa ggagattaaa gaagagagat tgtgagcctg
     9541 acgttcagca ccgagtcaca agaaacaaat tcaaaaaaca gcttctcata tttgttgaat
     9601 agtgtaactc gtgattcgga gttgacaaga gcaacaagaa gcgattttga agtatagctt
     9661 ctcgtatttt acaagatcgt gtcttattgc cgataggttg tctcttgaac accgtttatt
     9721 cgtaaaaagg gattgtgaca agtcttttgt cactacttac cgaacgaaac tgaaggagct
     9781 atccaaaaag tatcattagt attattcttt gtgtttctag tggcaccact agtacattta
     9841 aaaaagctat cgatcatgga ctaatcatac atgatcgata gctttttaga taatgtttgt
     9901 tacgctatct gaaattgact attataaaga ctggcataaa gcgttggtga ttggagtagt
     9961 tcagaatgtg ttcctttttc gacgatatcg ccattcttca ttacaaggat catgtctgca
    10021 ttttcaatcg ttgacaaacg gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct
    10081 tccattgctt gctgaatcaa tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct
    10141 aaaataacga caggaggatt cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt
    10201 ccttgagata acgtgccgtt ttcgctagaa atgatcgtat catatccgtc tggcagtgtt
    10261 cgaataaagt gatcacattg tgcgatttca gccgcttcga taatttgttc tcttgtggca
    10321 tccattttcc cgtaggcgat attatcagct accgttcctt caaacagcca ggtattttgt
    10381 aaaaccatcc caaaagaacg acgtagttct ccacgtgaaa gcttggtaat atcaagaccg
    10441 tcgaattcga tctttccttt attcagttca taaaaacgca tcagcagatt gactaaagtc
    10501 gtttttcctg caccagttgg accaacgatc gctaccgttt gttttggctt tactgaaaaa
    10561 tcgacttgat tcatcaatat tttatctgat gtgtaaccaa actgtacatt tttgaaagct
    10621 atttttcctt tcggttgttc taaaagtctt gcatctggaa ggtctggaat ttcatctgtt
    10681 tcatctaata tctcaaatat cctatcgatt gctgccattg cggattggat cgagttgatc
    10741 acataagaag cggtagaaat tggttcagaa atctgattga tgtactgaag ataagcttgt
    10801 aagaagccga tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg
    10861 atgaaggcga gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga
    10921 gcacgtttaa aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga
    10981 gcttgctgat tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta
    11041 tttaaaacac ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc
    11101 ttagttgtaa caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt
    11161 tcgatataaa acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga
    11221 ttgatacctg tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg
    11281 atgtcaccta cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg
    11341 atttcttttc gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga
    11401 ataaatgaag tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac
    11461 aatgcttgtt tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat
    11521 aagagatcat cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg
    11581 aataaactgc atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa
    11641 aaacgtatca aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct
    11701 atattccttg tgattttgca aactcctgat aagtttggca attagacagt agttcttcat
    11761 gagtaccttg tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga
    11821 ttgtactgag gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg
    11881 ctgctcttaa tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat
    11941 agatctctgc tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg
    12001 aataattaga accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta
    12061 gttcttctaa ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat
    12121 ttcccatctg caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat
    12181 aactgatgtg ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg
    12241 ttacttttcc tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc
    12301 cagatccggt cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat
    12361 cttctagaac tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg
    12421 catttgtctg atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga
    12481 tctctgcttg cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga
    12541 ccaataccat agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag
    12601 cagtcacgac accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa
    12661 tagaagcata aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc
    12721 ggttcacttt gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt
    12781 catatgattg attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg
    12841 tgatggtgat ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca
    12901 tcagtcgata aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta
    12961 atagagaata acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga
    13021 tcatttggca aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg
    13081 tcaaaagaga tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac
    13141 ggaaatattt ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac
    13201 tagaggcaac tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt
    13261 tttttataac tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt
    13321 ttggtacacc taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga
    13381 gtcctttgtt tttctgtaag aatgttttta agatagacat attattcctc cttttttcct
    13441 gatacttttc gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact
    13501 gaaaaagagg tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt
    13561 tgatttgcca aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag
    13621 acaagaagag aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat
    13681 cgttttttat agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac
    13741 attgacggag ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt
    13801 ggtgatgaaa caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat
    13861 cgatacaaac gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac
    13921 ggagtttgaa gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat
    13981 gatgagtatg ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga
    14041 agtacattta tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg
    14101 attcttattg aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga
    14161 aagaatgtat gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa
    14221 tacggatgaa aacaatatca gaactgaata ttatcatgca tggatagaaa tgaaaaaata
    14281 ggaaatatgt gtttaagata actcgaaaat gatggttgtt ttagttataa agaaaaattt
    14341 tctggttcga ttacgggttt aatgtggggg attattagtc gtggatagat agaattttag
    14401 ttttttagag gaaatatgtc tttaaaaatg gaatggttgc aattcttagc gatatatacg
    14461 atgtaattgt cctcttaaaa aagtatggaa aaataattgt attgggtgtt atgtataatt
    14521 catggtgaac tgagactaat gagaagaaaa attcatattg ttatgagtga gattgtatca
    14581 gatgagaatg ataaattaaa taggtgcata aaagcattac acttaaaata cattgttgct
    14641 tacattatta caatatgtta ataaggcaaa aataaataga tggattgaat aaacatattt
    14701 tttaatcaaa tagtccctac tgtgactt
//
LOCUS       DAZKAO010000066        14641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000066 DAZKAO010000000
VERSION     DAZKAO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.066"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG8H8_001683"
     CDS             complement(<1..270)
                     /locus_tag="ACG8H8_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP8986753.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCG"
     gene            543..3014
                     /locus_tag="ACG8H8_001684"
     CDS             543..3014
                     /locus_tag="ACG8H8_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8986754.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3117..4853)
                     /locus_tag="ACG8H8_001685"
     CDS             complement(3117..4853)
                     /locus_tag="ACG8H8_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8986755.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4850..5554)
                     /locus_tag="ACG8H8_001686"
     CDS             complement(4850..5554)
                     /locus_tag="ACG8H8_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8986756.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5685..6188
                     /locus_tag="ACG8H8_001687"
     CDS             5685..6188
                     /locus_tag="ACG8H8_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP8986757.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6148..6486
                     /locus_tag="ACG8H8_001688"
     CDS             6148..6486
                     /locus_tag="ACG8H8_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP8986758.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6507..7925
                     /gene="ffh"
                     /locus_tag="ACG8H8_001689"
     CDS             6507..7925
                     /gene="ffh"
                     /locus_tag="ACG8H8_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP8986759.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8037..8615
                     /locus_tag="ACG8H8_001690"
     CDS             8037..8615
                     /locus_tag="ACG8H8_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP8986760.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8567..9139
                     /locus_tag="ACG8H8_001691"
     CDS             8567..9139
                     /locus_tag="ACG8H8_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP8986761.1"
                     /translation="MFPQRRGRTYRQKGRIKHAKIIFYGAISLDGYLATKTDDLQWLF
                     DTPTGKQTTYEAFYETIDTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPD
                     AEVVNEDPVAFLKNLQMTEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIP
                     LFQKGTYEARFEMLDTTRFGEFIELHYVRK"
     gene            complement(9218..9772)
                     /locus_tag="ACG8H8_001692"
     CDS             complement(9218..9772)
                     /locus_tag="ACG8H8_001692"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP8986762.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10025..10300
                     /gene="rpsP"
                     /locus_tag="ACG8H8_001693"
     CDS             10025..10300
                     /gene="rpsP"
                     /locus_tag="ACG8H8_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP8986763.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10312..10563
                     /locus_tag="ACG8H8_001694"
     CDS             10312..10563
                     /locus_tag="ACG8H8_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP8986764.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10688..11479
                     /locus_tag="ACG8H8_001695"
     CDS             10688..11479
                     /locus_tag="ACG8H8_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986765.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11649..12170
                     /gene="rimM"
                     /locus_tag="ACG8H8_001696"
     CDS             11649..12170
                     /gene="rimM"
                     /locus_tag="ACG8H8_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP8986766.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12160..12921
                     /gene="trmD"
                     /locus_tag="ACG8H8_001697"
     CDS             12160..12921
                     /gene="trmD"
                     /locus_tag="ACG8H8_001697"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP8986767.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13057..13404
                     /gene="rplS"
                     /locus_tag="ACG8H8_001698"
     CDS             13057..13404
                     /gene="rplS"
                     /locus_tag="ACG8H8_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP8986768.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13440..13625)
                     /locus_tag="ACG8H8_001699"
     CDS             complement(13440..13625)
                     /locus_tag="ACG8H8_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986769.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            13662..13955
                     /locus_tag="ACG8H8_001700"
     CDS             13662..13955
                     /locus_tag="ACG8H8_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP8986770.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(14043..14189)
                     /locus_tag="ACG8H8_001701"
                     /pseudo
     CDS             complement(14043..14189)
                     /locus_tag="ACG8H8_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         4888 a         2636 c         2968 g         4149 t
ORIGIN      
        1 tccacaatca tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt
       61 gactttcaac atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg
      121 acagtgttca caataggaag aagagtcatc caattttcct ttaataaact gcgcttgaac
      181 gcctttatat tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa
      241 tccgagtaat tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt
      301 ggtcgactaa attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt
      361 agtagaaaat cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg
      421 ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg
      481 ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc
      541 acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag
      601 ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg
      661 aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc
      721 aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt
      781 tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc atagccaata
      841 caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt
      901 taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag
      961 accttgtaat agatgacatt gttattttag agactggata tcaaattcca gcagatgcag
     1021 aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa
     1081 taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt
     1141 acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca
     1201 agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat
     1261 ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta
     1321 atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac
     1381 ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa
     1441 agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc
     1501 tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg
     1561 tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact
     1621 atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa
     1681 aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca
     1741 gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc
     1801 aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg
     1861 tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat
     1921 taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact
     1981 tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca
     2041 atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat
     2101 cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag
     2161 aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg
     2221 atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg
     2281 gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca
     2341 tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct
     2401 tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc
     2461 tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc
     2521 catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga
     2581 atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg
     2641 tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc
     2701 ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca
     2761 gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata
     2821 agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt
     2881 tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta
     2941 aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta
     3001 aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta
     3061 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
     3121 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
     3181 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
     3241 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
     3301 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
     3361 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt
     3421 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
     3481 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
     3541 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
     3601 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
     3661 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
     3721 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
     3781 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
     3841 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
     3901 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
     3961 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
     4021 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     4081 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     4141 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     4201 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     4261 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     4321 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     4381 ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     4441 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     4501 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     4561 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     4621 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     4681 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     4741 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     4801 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     4861 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     4921 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     4981 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     5041 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     5101 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     5161 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     5221 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     5281 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     5341 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     5401 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     5461 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     5521 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     5581 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     5641 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     5701 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     5761 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     5821 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     5881 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     5941 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     6001 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     6061 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     6121 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     6181 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     6241 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     6301 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     6361 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     6421 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     6481 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     6541 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     6601 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     6661 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     6721 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     6781 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     6841 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     6901 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     6961 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     7021 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     7081 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     7141 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     7201 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     7261 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     7321 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     7381 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     7441 atgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     7501 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     7561 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     7621 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     7681 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     7741 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     7801 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     7861 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     7921 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     7981 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     8041 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     8101 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     8161 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     8221 tcgatattaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     8281 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     8341 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     8401 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     8461 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     8521 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
     8581 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaatca tattttatgg
     8641 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
     8701 cactccaaca ggtaagcaaa ccacttatga agctttttat gaaacaatcg atacgaccat
     8761 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
     8821 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
     8881 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
     8941 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
     9001 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
     9061 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
     9121 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
     9181 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
     9241 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
     9301 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
     9361 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
     9421 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
     9481 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
     9541 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
     9601 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
     9661 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
     9721 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
     9781 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
     9841 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
     9901 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
     9961 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
    10021 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
    10081 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
    10141 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
    10201 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
    10261 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    10321 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    10381 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    10441 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    10501 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    10561 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    10621 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    10681 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    10741 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    10801 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    10861 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    10921 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    10981 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    11041 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    11101 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    11161 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    11221 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    11281 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    11341 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    11401 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    11461 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    11521 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    11581 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    11641 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    11701 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    11761 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    11821 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    11881 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    11941 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    12001 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    12061 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    12121 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    12181 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    12241 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    12301 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    12361 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    12421 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    12481 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    12541 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    12601 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    12661 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    12721 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    12781 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    12841 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    12901 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    12961 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    13021 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    13081 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    13141 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    13201 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    13261 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    13321 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    13381 cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt
    13441 caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt ttctttaatt
    13501 attggtaggg gagaataatg gattagaata aatctaccta taagtagtag taaaaaaaag
    13561 agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc agaatattct
    13621 aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat acttatacgg
    13681 agcaagaaaa gatgaaagtt ttagacacat tcatgaatga tggaaaagta aaacaagtac
    13741 caagttcaac aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc tttgaccatg
    13801 gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt tctagtggcg
    13861 attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga agttcagacg
    13921 gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata ctcattgaaa
    13981 aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt tgtcatctgt
    14041 atctataata caaagccaag aacgttattc aaaatatgga tagctatgat aaaagctaat
    14101 gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa
    14161 aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta cgaataaata
    14221 ccaaaacgta cctcctaaaa ttagattttg gacagatgcc ctttaaaaag ataacaagat
    14281 ttcgttcttg aaacaatata tatgctttta ttccaatgag gttgtgaaaa cagtattttg
    14341 tcctgagcgt tagaaaattg aaaatgggga atagcaactc gtactttacc attttttaag
    14401 tgaatacaag atcagctaat tgaacaccgt ttattcgtga tgagggacac aacactgcag
    14461 tcgacacatt tctcttttat gttcaaagat aacaaatatt taaaacaaag aaaaatagta
    14521 gataatcccg tcctaactct tttattcttc cctcatagct tccaaattta agcaatactt
    14581 caattaatca agttttatct ttttttttca ataatcgtaa aaaaagagta ttttttattt
    14641 a
//
LOCUS       DAZKAO010000067        14562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000067 DAZKAO010000000
VERSION     DAZKAO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.067"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            409..741
                     /locus_tag="ACG8H8_001702"
     CDS             409..741
                     /locus_tag="ACG8H8_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8986771.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            651..854
                     /locus_tag="ACG8H8_001703"
     CDS             651..854
                     /locus_tag="ACG8H8_001703"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8986772.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            885..1031
                     /locus_tag="ACG8H8_001704"
     CDS             885..1031
                     /locus_tag="ACG8H8_001704"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986773.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1469..2809
                     /locus_tag="ACG8H8_001705"
     CDS             1469..2809
                     /locus_tag="ACG8H8_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8986774.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(3050..3457)
                     /locus_tag="ACG8H8_001706"
     CDS             complement(3050..3457)
                     /locus_tag="ACG8H8_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP8986775.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(3646..4656)
                     /locus_tag="ACG8H8_001707"
     CDS             complement(3646..4656)
                     /locus_tag="ACG8H8_001707"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP8986776.1"
                     /translation="MPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVNVDG
                     VLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPI
                     PIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEE
                     QDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSK
                     ILNTNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLT
                     DKNFPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKN
                     PN"
     gene            4782..5000
                     /locus_tag="ACG8H8_001708"
     CDS             4782..5000
                     /locus_tag="ACG8H8_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986777.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5093..5656
                     /locus_tag="ACG8H8_001709"
     CDS             5093..5656
                     /locus_tag="ACG8H8_001709"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8986778.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5849..6088)
                     /locus_tag="ACG8H8_001710"
     CDS             complement(5849..6088)
                     /locus_tag="ACG8H8_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986779.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6112..7635)
                     /locus_tag="ACG8H8_001711"
     CDS             complement(6112..7635)
                     /locus_tag="ACG8H8_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP8986780.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(7653..7775)
                     /locus_tag="ACG8H8_001712"
     CDS             complement(7653..7775)
                     /locus_tag="ACG8H8_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP8986781.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(7805..8788)
                     /locus_tag="ACG8H8_001713"
     CDS             complement(7805..8788)
                     /locus_tag="ACG8H8_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP8986782.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8812..8961)
                     /gene="rpmG"
                     /locus_tag="ACG8H8_001714"
     CDS             complement(8812..8961)
                     /gene="rpmG"
                     /locus_tag="ACG8H8_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8986783.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8982..9359)
                     /locus_tag="ACG8H8_001715"
     CDS             complement(8982..9359)
                     /locus_tag="ACG8H8_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986784.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9391..9570)
                     /gene="rpmF"
                     /locus_tag="ACG8H8_001716"
     CDS             complement(9391..9570)
                     /gene="rpmF"
                     /locus_tag="ACG8H8_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP8986785.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9585..9854)
                     /gene="rpsN"
                     /locus_tag="ACG8H8_001717"
     CDS             complement(9585..9854)
                     /gene="rpsN"
                     /locus_tag="ACG8H8_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP8986786.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10377..12143)
                     /locus_tag="ACG8H8_001718"
     CDS             complement(10377..12143)
                     /locus_tag="ACG8H8_001718"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986787.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12103..13857)
                     /locus_tag="ACG8H8_001719"
     CDS             complement(12103..13857)
                     /locus_tag="ACG8H8_001719"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986788.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13967..14536)
                     /locus_tag="ACG8H8_001720"
     CDS             complement(13967..14536)
                     /locus_tag="ACG8H8_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP8986789.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4648 a         2829 c         2222 g         4863 t
ORIGIN      
        1 ttctataatg attcatagaa tctaaaacga atgattttaa ttattaacgt tttttaattc
       61 gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt
      121 acgaagtggg caggaggtcg aatccagaac tgggccatgg tacttcaacg aggacttctc
      181 ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat
      241 gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa
      301 ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga
      361 tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt gaaaaaaaca
      421 tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc
      481 aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa
      541 tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca
      601 gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg
      661 aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg
      721 aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat
      781 ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaaa
      841 tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgacg gatattactt
      901 acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta gcaactcgta
      961 ttctacgtta ctatatggtg gattcacaca tgaaaaaaga gattgtcgtc cgtccattac
     1021 aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc cagcatcggt
     1081 cttttattta tcaagagctg ttgaccgacc accacatcac acattcaatg agccaaactg
     1141 ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa
     1201 tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa gattatttag
     1261 aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct tgacaatttg
     1321 aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct gttgccaata
     1381 ctttttcttt tttctaataa acgcaaacag acaccttgaa atcctttaga ataaagtcga
     1441 cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca aagagttact
     1501 agatattcta gacctaaatc ttacctttca tgaagatgcc tttcgcaaag agcgcattaa
     1561 tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccagagg aatgcttcca
     1621 ttgccactat caaaataagc aaacgatcat taaatggggc tggaaaaaag tatccatctt
     1681 attgaatgat gtcagtaact acaagaccat tgtgcggatt aacaaacagc gcttcaaatg
     1741 taaacactgt ggaaagactt ttttagcaga agattcagtt agcgatcgtc gctgctccat
     1801 tgcccgcaga gtcaaacaag ccattcttga gctattaagt gagcctattt ctatgtcttt
     1861 gattgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc gcagccttcg
     1921 gcctaaaacc gtttctttga accagccgtt accagaagtg gtgtgtttcg atgaattcaa
     1981 atcggtgaaa aacgtctcgg gagccatgag ctttgtcatg atggacggca agacccatca
     2041 actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt tcttgcgcta
     2101 ccctagaaaa gtccgtgaac aagtacgtct agtggtttca gatttctata caccctatcg
     2161 cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct ttcatattag
     2221 ccaacacatc ggtcgggcct ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa
     2281 aggggatcgt cggtcgaagc acttaaagaa atactggaga ctacttcaaa agaatgcgtg
     2341 ggaattgaat ggtcagcatc gctactggcg tccgtcattt cgagatcatc tgacagaagc
     2401 ggagatagtc gatcggctat tatattatga tgactcctta aagcgaggct acgaagtcta
     2461 ccaggttttt ctttccgcta tcagaagaca agatgttcct gaattcgtcg cgctcctaaa
     2521 agaagactac aaggagttgc cagagcacta tcaaccagtg ttcacgacct tcaagaaata
     2581 tcgaacagaa atcaaacgag cgttgcgagt tccctattcc aacggcccta tagaatgttt
     2641 gaacaaccat atcaaagtac taaaacgaat tgcctatggc tttagaaact ttcaaaacta
     2701 ccgagaacga atctttttat atcgaggaaa atatttcaag aaaactaaga acactaccca
     2761 actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc cgtgtgagta
     2821 tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc aaaataagta
     2881 atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg tgcctgttgt
     2941 caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc ctgctattat
     3001 taataataat atctttttct tattaaaaac ctcctcatct atttccagat tagtaaatgt
     3061 agtaatcttt gctttctttt ttctcaatac taataactaa cttatggggt aaacaaatac
     3121 tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca ggactattat
     3181 cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt cccttcgcag
     3241 gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc atttccttgc
     3301 catcaatttg aacgatggca tatttgatag catcttcagt tgtttcattc tccttttcta
     3361 tagagaatac aaataaggga ataaaagagg gaataatcaa aatgccaata attatgacat
     3421 cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg ctaaacatat
     3481 tagaattttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta agagaatcag
     3541 ttaaacaacc ctcaagttgt tccttaatac attgttgatc catcttcata ccaataaaaa
     3601 ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat tcgggttttt
     3661 ttgtataatc atttgacact caattttagg taaactagct acccaataat ttaatgattc
     3721 aatactttta tggctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa
     3781 ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc
     3841 aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc
     3901 ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccattga
     3961 ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc
     4021 acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat
     4081 tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca
     4141 ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc
     4201 agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc
     4261 cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt
     4321 tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat
     4381 aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc
     4441 gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt
     4501 aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat
     4561 gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc
     4621 tgaaccaaga tagccagtta agattgtaat aggtattttt tcatagggaa cctctttcta
     4681 taccttgaat ttacacttta agtgcaacac ataaaaatga gtcataatcg atatccaact
     4741 agtataagtt tgtcgagaac actcctagaa ggaaaataat tatgagccag tattgtcatc
     4801 ttactatact caaacgagaa agaatctttc tgcttcacga agaaggattt tctattagta
     4861 taattggcaa cgacattcag catagcccgt ttactatttt acatgaactg agaagaaaca
     4921 agaaaaaaac taatcacctt ccaaagcgga aaaaaattat gcttatcgaa gaaaaaaatg
     4981 cggacgttat cacattctaa atgatccgtg agtttggtca attgttcgtc atttatttgt
     5041 cgattttcag tggtcgccag agaaaatctc gaatcgtttg aagcatgaac atatggactt
     5101 ttcaattagt tttatcacga tctaccgtgc aatttgcaat gatcttttcg atacagaacc
     5161 cctctcacac ggcaataaag gcctgatccg ttcattgcac catcgaggaa aaacaagaca
     5221 tacaaaaaat catgtcgagt ctcgtgggaa gatttgcctt actcattcga tccatggcca
     5281 tccaaaatcc gcagatgata gaagtgattt tggtcattgg aaagccgata ttgtagctgg
     5341 caaaactagt ggtacttgtt tagtgacttt gacagacaga aaatcatgct tccttttagc
     5401 aggaaagaag gtccccagaa agaaagccgc ttatgtgagc gagaaaatga ttgaactttt
     5461 aagcagtatt ccaaatgagg cggtcaaaac gatcactcca gatagaggaa aagaattctc
     5521 caatcatttc gcagttacag aagtattgaa taatatgccc ttttattttc cagatcctca
     5581 tgcccttggc aacaatgaac aaattaaaat acgaatagac tcattcgaga atacttgcca
     5641 aaatcgaaag agatagctac ggtagataac tcaacgataa atcagatgat tgaaaaactg
     5701 aataacagac ctagaaagtg tttagattgg agaacacctt atgaagtctt ctttgataaa
     5761 gtgttgcgct tgatttgaca attcaagaaa taataaaaag cagatcaaat ccaaataagg
     5821 gaaaaatgat ctactctggt attatcaatt aattggccaa gctattatgc cgttttgcta
     5881 aacgtctact tcttccagta ttgaccttga taaaacaaca atcaggatcc cgttttaata
     5941 ctctatgaac tgtgtcaata ctaacatgta aagttgcagc aatatcagct aatgtactcc
     6001 catttaatct catttgcttt gcaaattgat aacgccgata agcatcaata ttcttggaat
     6061 ccatagacaa taatactttt cttaccatgt atttcctcca aagattggat tttaatgggc
     6121 catcatttct tgagcaatct catgctcaga taatgaagct ggatagaaag taggccaatt
     6181 atgcatttca ttataaagtt tttcttgact ttctccacca aagaaaatat ggaaatgctc
     6241 tgccttactt ggtgcaattc catgatcgct aaattgaata tatttaaaac gaccagcacc
     6301 tgcatcatca gtttcaaaat taaaacgaac accacggttt cctttttcat aattcaaaat
     6361 tttgtatcca acatatttgt atgtgtattg atgatgttca ccgttgacta caaaatcaat
     6421 ggtgttgtct ttaatgttga tagtatcaat atctgtctta tagccagtag tataataatc
     6481 tttgtactcg gcagctgtca tgtctttatt gagttttgct ttgtaatcga aaacttcatc
     6541 caaaattcca tcttttaaca acggataaac agattgccat tcgcctgtat aatcactcaa
     6601 ttcgcgatcc ttcacatcgg catctttaaa gtacccatta gcaactgtct tttcatcctt
     6661 cgcttctttt tctggaacta cctcttcacc agcaaccata gttgttttct ctaacgcttt
     6721 tagattgtct tccattactg aaacataatc ttcaccatta tccatttgtt cttttgtaag
     6781 actttcaaga ggattcaata cttccaattt tatcccagct tcattggcca aagtttttgc
     6841 gattttatca ttagcattct tttcaaagta aatataattg atcttatttt ttttaacata
     6901 ctcttttaat tcagctaatc gaccagaaga tggctcttct tcaggactta gtccagcaat
     6961 aggaacttgt atcaatccat aatccaaagc taaatatcca aaggctgcat gttgtgtcac
     7021 aaaacttttt tgtttcgctt ctttaagact agtggtatag tcagcatcca accgtttcag
     7081 tttagtcaaa tacttttcag catttgtctc aaatactttt gcttttttcg gatacaattt
     7141 aactaactga tccttgatat ttgaaacttc cttaattgcc attttgggtg aaacccacgt
     7201 atgaggatct agttcatgat ggtgatcttc ttctccgtga tcatgatctt cttcttcact
     7261 acctggcaat aaaatcatgt cttttgttcc ttctactaca ttaggcccag ctttcttcca
     7321 actttcttta gcttttggta cccatgattc catgttttcg ttatgatata caaaaacatc
     7381 agcatcatga gcttttgcca tatccttagc tgagggctca tagtcatgag gttctgatcc
     7441 cgctggaacc attaaatcca cgttcgcttc atcgccgacg atgtttttgg tgaaatcata
     7501 cattggatag aatgttgtta caatttctaa ttttccatct ttatcattcg atacttttgc
     7561 atcattagtg ctggagcaag ctcccaataa tcctagagct gctatcccaa taagtcctac
     7621 actgattttc ttcattcttt ttccttcctt aattatgcta atttcttcat tttattttgt
     7681 ttatgttctt tgataatttt taatgccctt tttctagttt tgatattcgg gcttttaagt
     7741 cgtcgatatg cactagatat atccgttttc tccattagaa attaccccta atcaatactt
     7801 atcattattt gctttccgcc agtaatttat caaaactctg tttgatcctt tcttcatcca
     7861 acatttttcc aattagaata atagtggttt ggtctttttg tcgagtggtg tattgaaaat
     7921 taacctgttc gtttacccct tgtatggcaa ctataaaatc ttgatcttgc aaagcaatca
     7981 atcctttaaa acgatacagg ctcccttggt tggttaaaat cagccggtca attaattgga
     8041 ataaaaaatc tttttttatg tcttgatctg aagttaagag aattgattta aactcatgtt
     8101 cttgatgatg ttgatgatag tgtttgccat tatagtgatt ttctgatgaa tcactttcaa
     8161 aaactaaggg atgattgaat ttttccaaat tataaaaatc ctttttttga atcgtttcac
     8221 tatgtgtgaa cattcgaata tcggataaag ggttaatact tttgatttct ctctgtaatt
     8281 tttttagtgc agataaattc aagttttgtt ttctagacac aaaaatgcga tctgctatga
     8341 caagttgctt aagggcttgt ggttctcttt caataagttc aaaactgtca ctatcaataa
     8401 cagtcaataa actatcgata tatactcttc ctcttagttg aggcgctgtt aaaagtgttt
     8461 gaatgattgg ttggggatct gctatacctg tagtttcaat gatgatttgt gaaattggtt
     8521 gttgatgttc ttctattgtt tccagtaaag aaatcaatat tgctaataaa tcactgcgaa
     8581 gactgcaaca catacatcca ttatttagtt gtaacacccg ttcctcgcta tgaactaata
     8641 atttgtgatc aatcccaact tcaccaaact cattttcaat tataataatt tcttccctgg
     8701 ggtagggtga gacttgcaag gcttgattga ttagcgttgt ttttccagcc cctaaaaacc
     8761 cagaaacgat tgtaactggt atgcccattt tgtcaccttt ttaccactta gctatttcac
     8821 ttctttgaaa attgcccgtc tgcgcaattt tggcgaatac ttttttaatt ctaaccgttc
     8881 agggttattc cgtttatttt tgctggtgag atacagacgt tctccagttt cgacacattc
     8941 caaaataata ttttgacgca taaatcttcc tccttcaata cttatcgatc ttcaagatat
     9001 gcttgaattt gttgtgcatc ttcaaaagtt aactcgccag attccaattg tgaaactaaa
     9061 ttttctttca tatcaacagg tatctcccaa taaccatcaa tacattgaaa taactcctcg
     9121 tcatactctt caaaaattgg atagattctt aaaaaatcat caaatgataa gccttggtcc
     9181 tcatagtaat ctttgactaa tccattttca attgcctgtt gggtcggtaa acccgtttca
     9241 gtaataattc ctctctctac catcgaacga taaaaagtgc atttatctag cgattgctgg
     9301 gtcgtttcaa gataatactt taatccttct tcaactaaag gtgggtaaat aatttccatt
     9361 ttgattccct cctaaaagtt atatattaaa ctattttgaa gctcctttaa ttactttctt
     9421 tccattatag taccctttta aagatacatg atggctacgt ctataatcac cattcgcttc
     9481 gtcaaatgaa atttctggtt ttactacagt ttgatgagtt cttcttaacc gtttcttcgc
     9541 cttagatgtt tttcttgctg gtactgccat gatatttcct ccttttacca acttgctttt
     9601 tttactccag gaatcaaccc ttgatgggca agttctctaa acttaatacg ggacataccg
     9661 aactttcgca tataccctct tggacgacca tccgtctgat cacgaagcct cagacgattc
     9721 ggatttgaat ctcttggcaa tttagcgaga gcggtgcgat ctcctgcggc ttttaattcc
     9781 tgacgtttat ctgcgtactt ttcaatcatc gccatttgtt tctttgcttt agcaattttc
     9841 gacttttttg ccatatacct ttatactcct tttattttga tttagacatc tcaaatcgta
     9901 atcgttacgt tttgacactg atactttacc aacaagagtt gctcttgtca acaataaata
     9961 acatgaaata aaactcatat gaatagataa ctatttgaaa acgaatttat cggtacatag
    10021 agataatcaa atcatttaaa agaatatgca gtttaagctc tgaaaaataa gaattcactt
    10081 ttattagtcc aatcaaacac aaataccgtt ttaagtagct tttcctctac ttaaaacggc
    10141 aatatgaaag aaactataac tttttattca atcttttatg tttaactggt ttatcgccaa
    10201 ttttttaaaa ctttgattat tccttatatt ataagaatag acattaagtg aactaattca
    10261 atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt
    10321 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
    10381 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
    10441 tttttgtata tttctgacct tcgatacaac tcactatgag taccttgatc aactattttt
    10501 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
    10561 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
    10621 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
    10681 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
    10741 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
    10801 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
    10861 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
    10921 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
    10981 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
    11041 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
    11101 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
    11161 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
    11221 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
    11281 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
    11341 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
    11401 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
    11461 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
    11521 tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt
    11581 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
    11641 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
    11701 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
    11761 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
    11821 gacaaatcgt ctgcaatttg cattcttaaa tgcgcacttt ctttataagt actattatat
    11881 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac gaacgatact
    11941 gtaaaataaa gccaaggcgg ctttacattt tctaaaacta attcgtctat cagcatcatt
    12001 ataatgaatg ctggtaccat attaatgcag tatacaaaaa agctagaaat agatgcttta
    12061 accaaatctt ctgcgccctg attagttaaa gcgaatttgt ttttataaaa acttttcatt
    12121 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
    12181 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
    12241 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
    12301 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
    12361 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
    12421 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
    12481 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
    12541 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
    12601 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
    12661 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
    12721 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
    12781 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
    12841 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
    12901 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
    12961 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
    13021 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
    13081 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
    13141 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
    13201 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc ctcggatata
    13261 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
    13321 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
    13381 tccaatttta aaattaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
    13441 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
    13501 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
    13561 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
    13621 aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac
    13681 cgcataactc ttagctaaat attcatctcc tacaataatt agagacttaa gaaatttgta
    13741 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
    13801 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
    13861 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
    13921 tagcgtaaat tagagatgta caaacagata gaaggtaaac tacgttctat tttttgtatt
    13981 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
    14041 ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag
    14101 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
    14161 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
    14221 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
    14281 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
    14341 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
    14401 ataataatcc aaaaatccct ggggatatgc tgtgaccaaa cgccccacca atactactaa
    14461 ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat
    14521 aatcttttgt tttcatatat attctcctca attctatttc gt
//
LOCUS       DAZKAO010000068        14437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000068 DAZKAO010000000
VERSION     DAZKAO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.068"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1113)
                     /locus_tag="ACG8H8_001721"
     CDS             complement(226..1113)
                     /locus_tag="ACG8H8_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986790.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="ACG8H8_001722"
     CDS             complement(1110..1979)
                     /locus_tag="ACG8H8_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8986791.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="ACG8H8_001723"
     CDS             complement(2116..3402)
                     /locus_tag="ACG8H8_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8986792.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="ACG8H8_001724"
     CDS             3675..4253
                     /locus_tag="ACG8H8_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8986793.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="ACG8H8_001725"
     CDS             4253..5182
                     /locus_tag="ACG8H8_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986794.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="ACG8H8_001726"
     CDS             5179..6915
                     /locus_tag="ACG8H8_001726"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8986795.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8H8_001727"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8H8_001727"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP8986796.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="ACG8H8_001728"
     CDS             complement(7811..8320)
                     /locus_tag="ACG8H8_001728"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP8986797.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8H8_001729"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8H8_001729"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP8986798.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="ACG8H8_001730"
     CDS             complement(9621..10712)
                     /locus_tag="ACG8H8_001730"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP8986799.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8H8_001731"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8H8_001731"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP8986800.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8H8_001732"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8H8_001732"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP8986801.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8H8_001733"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8H8_001733"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP8986802.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2608 g         4573 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgat
//
LOCUS       DAZKAO010000069        14283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000069 DAZKAO010000000
VERSION     DAZKAO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.069"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8H8_001734"
     CDS             4..2376
                     /locus_tag="ACG8H8_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP8986803.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8H8_001735"
     CDS             2437..3897
                     /locus_tag="ACG8H8_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP8986804.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8H8_001736"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8H8_001736"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP8986805.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG8H8_001737"
     CDS             6128..6277
                     /locus_tag="ACG8H8_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986806.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG8H8_001738"
     CDS             6352..6945
                     /locus_tag="ACG8H8_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP8986807.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG8H8_001739"
     CDS             6958..7857
                     /locus_tag="ACG8H8_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP8986808.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8H8_001740"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8H8_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP8986809.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG8H8_001741"
     CDS             8723..8983
                     /locus_tag="ACG8H8_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986810.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG8H8_001742"
     CDS             9428..9634
                     /locus_tag="ACG8H8_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP8986811.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG8H8_001743"
     CDS             9655..9885
                     /locus_tag="ACG8H8_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986812.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG8H8_001744"
     CDS             9901..10338
                     /locus_tag="ACG8H8_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986813.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG8H8_001745"
     CDS             10331..11038
                     /locus_tag="ACG8H8_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986814.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG8H8_001746"
     CDS             11202..11402
                     /locus_tag="ACG8H8_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986815.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG8H8_001747"
     CDS             11418..11597
                     /locus_tag="ACG8H8_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986816.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG8H8_001748"
     CDS             11922..12185
                     /locus_tag="ACG8H8_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFP8986817.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG8H8_001749"
     CDS             12179..12529
                     /locus_tag="ACG8H8_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP8986818.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG8H8_001750"
     CDS             complement(12553..13167)
                     /locus_tag="ACG8H8_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8986819.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG8H8_001751"
     CDS             13481..13903
                     /locus_tag="ACG8H8_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986820.1"
                     /translation="MKNKFILSLGILTIGVSVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG8H8_001752"
     CDS             13913..14116
                     /locus_tag="ACG8H8_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986821.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT         5296 a         2333 c         2878 g         3776 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggagttag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atg
//
LOCUS       DAZKAO010000070        13962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000070 DAZKAO010000000
VERSION     DAZKAO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.070"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..978
                     /locus_tag="ACG8H8_001753"
     CDS             130..978
                     /locus_tag="ACG8H8_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP8986822.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1149..1490)
                     /locus_tag="ACG8H8_001754"
     CDS             complement(1149..1490)
                     /locus_tag="ACG8H8_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP8986823.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1576..2283
                     /locus_tag="ACG8H8_001755"
     CDS             1576..2283
                     /locus_tag="ACG8H8_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP8986824.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2270..2593
                     /locus_tag="ACG8H8_001756"
     CDS             2270..2593
                     /locus_tag="ACG8H8_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP8986825.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2682..3719)
                     /locus_tag="ACG8H8_001757"
     CDS             complement(2682..3719)
                     /locus_tag="ACG8H8_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8986826.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4166..5149)
                     /locus_tag="ACG8H8_001758"
     CDS             complement(4166..5149)
                     /locus_tag="ACG8H8_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP8986827.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5565..6335)
                     /locus_tag="ACG8H8_001759"
     CDS             complement(5565..6335)
                     /locus_tag="ACG8H8_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986828.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6328..7263)
                     /locus_tag="ACG8H8_001760"
     CDS             complement(6328..7263)
                     /locus_tag="ACG8H8_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986829.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7857..9506)
                     /locus_tag="ACG8H8_001761"
     CDS             complement(7857..9506)
                     /locus_tag="ACG8H8_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP8986830.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9701..10597)
                     /locus_tag="ACG8H8_001762"
     CDS             complement(9701..10597)
                     /locus_tag="ACG8H8_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8986831.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFHTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10590..11270)
                     /locus_tag="ACG8H8_001763"
     CDS             complement(10590..11270)
                     /locus_tag="ACG8H8_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8986832.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11271..12053)
                     /locus_tag="ACG8H8_001764"
     CDS             complement(11271..12053)
                     /locus_tag="ACG8H8_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8986833.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12046..12963)
                     /locus_tag="ACG8H8_001765"
     CDS             complement(12046..12963)
                     /locus_tag="ACG8H8_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8986834.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12966..13250)
                     /locus_tag="ACG8H8_001766"
     CDS             complement(12966..13250)
                     /locus_tag="ACG8H8_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986835.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4133 a         2989 c         2454 g         4386 t
ORIGIN      
        1 aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa atatgtaaaa
       61 tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt ttcatgaaag
      121 gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga acgcggttac
      181 agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta cttgtccgca
      241 acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa gaccaacatg
      301 acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc tgctgtctta
      361 ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga actctcgtcc
      421 cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat tcttatagaa
      481 catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat aaattattca
      541 gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa caaaaacagg
      601 aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt tgaatcgctt
      661 attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat tgctactctc
      721 gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga ggactgttat
      781 tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca aatgttctct
      841 tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta ctctataaaa
      901 atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa
      961 aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta aacgataaca
     1021 accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga atcatgcaaa
     1081 ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta ttaggtacgc
     1141 gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt ccggcattgt
     1201 tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc atctgtatat
     1261 tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc tagtgcagca
     1321 atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac cgttgcatcg
     1381 aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc caccgaaaca
     1441 atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat tgacaaatgc
     1501 tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt atgtcgtgct
     1561 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     1621 cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     1681 ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg ttctgcccaa
     1741 tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt tttctcgacc
     1801 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     1861 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     1921 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     1981 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     2041 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     2101 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     2161 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag caacttagtg
     2221 attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt
     2281 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctagggtcct
     2341 accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt atttaagttt
     2401 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     2461 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     2521 tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc tttcgttgat
     2581 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     2641 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     2701 ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc ttgtttcaag
     2761 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     2821 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     2881 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     2941 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     3001 tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
     3061 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
     3121 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
     3181 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc gttgatcact
     3241 aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt ttcaaataac
     3301 aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc tgaaagattt
     3361 tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt gcttcctgct
     3421 cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcgtca
     3481 cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc atttcttatc
     3541 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
     3601 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
     3661 tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
     3721 cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt tcaatatata
     3781 tagtatgcta tttgttccac cttttttatt catcataatt attctcttac ttaaagtaaa
     3841 ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca caacctctta
     3901 taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattcatc aaacatgaga
     3961 agctgttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctg
     4021 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
     4081 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
     4141 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
     4201 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
     4261 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
     4321 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
     4381 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
     4441 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
     4501 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
     4561 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
     4621 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
     4681 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
     4741 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
     4801 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
     4861 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
     4921 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
     4981 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     5041 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     5101 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     5161 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     5221 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     5281 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     5341 catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt gtttggctgt
     5401 tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg tgttcaatca
     5461 gctgaccctc ggtattcgct caaaaaacaa caaaatatga ggaactattt tcccgtttgt
     5521 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact tctcaatcct
     5581 cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa taacacaagt
     5641 acgatcatcg atgttgctac tggcacatcg atcgttccaa ggaatccgta acggaaaccg
     5701 gagatcatat aaacgatcgg attgactttt gaaacagctt gccaaaaggg cggtaacata
     5761 gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa tgtaggcacg
     5821 atcgatacat catcataaga ctgagcaaaa atcccgttga gcagtcctgc taatgaaaac
     5881 aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac atgaagtggt
     5941 acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg acccacacca
     6001 ccaatcacaa agccccaaat aatcacatgc gtaggaacag gtgctaccaa gatttcttcg
     6061 atatttttct gaaatttttg agagaagaag gacgaagaaa cattggcata cgaactagtg
     6121 atagcagaca tcataatcaa accaggaacg atgaattcca tatatgaaaa accacccatg
     6181 tcaccgatcc gcccaccgat catttttccg aaaataacaa aatacaagga tgtcgtgatc
     6241 actggcggaa ccagtgtttg gacccatata cgcaaatagc ggttcgtttc cttgatagct
     6301 aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat gattattttc
     6361 ttctgtgatt ttcaagaaca gttcttctaa acgattcgac ttattcctca tagaaagaac
     6421 tttgattcct tgtcttgtaa gctgatcgaa gattccgttc accccttgat ttcgttcaac
     6481 ttcgacagcg attgtctgct catcttcaaa tgtacaagca tatccttcaa tcacaggttt
     6541 ggtatcatat ggcgctaaat caaagataaa tgtttcaaat tgcagttttg acagaagctc
     6601 tttcatactt gtattctcga tcagttcacc cgattggatg attccgatgt tgcgacaaag
     6661 catttcagct tcttctagat aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa
     6721 ttctcgtaga aactcccaca tctcgcgtct caactcgatg tcaactcctg ctgttggttc
     6781 atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca gtcttcgctt
     6841 cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat tggactgctt
     6901 caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt agcctgcttg
     6961 attcacgacg atttgctgga ccgtttcaaa agggttaaaa ttgaactctt gtggaaccaa
     7021 tccaatctgt tgctttgctc gaactagatc cttgtccaga tcataaccga agacattcac
     7081 ttttccagat gttttattga ctaaagaagt aataatcccg atcgttgtag acttcccagc
     7141 tccatttggc cctaatagcg catagaaatc tccttctttc accgaaagat cgattccccg
     7201 caatgcttcg accccacttc catagatctt tttcaaatta tttatttcaa gcgcatttgc
     7261 catttatttc ctatctcctt atagacatga ttcttaattg tatcatggaa tacagcagat
     7321 gaaaaaacaa cttggcttcc acacaaaaac acacctgcta acaaagagga gatgtgtttt
     7381 ctagttcatg atcatcttcg atagaataac tatctaaaca actctggaac ttttaaaatc
     7441 ataaaagtac cgacagcaac tactacgatg acatatagaa gatccttata actgaggttc
     7501 ttgtcttttt tattcatgtt aagctttcct ccgtcctttt atcgaaagct tttttctttt
     7561 attttaaccg aaataaataa aatggaaata gttattttca atttctgttt tcgctgtttg
     7621 ttttacggag aactttgcca tggaacgtgc gtctcgtcgc tgatgctaag ttttttcgat
     7681 aaaagctgct agaaggaaga cagcgtggtt tattttgtat cgtcttttgc taaatcttcc
     7741 aagatcatct ggacagcaga ctcgttccca ttgaattctg ccactttttt ggcatcaatg
     7801 tacttctttt gataatcgtc tttttggacc cacgcatcat gcttcgtttg ataaagttaa
     7861 tcaaacggat accactgacg ctgttcatcg aacaacaaac tttctttttg ggcctgttca
     7921 actatggatt taggataatt caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
     7981 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
     8041 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
     8101 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
     8161 cgttcgatcg tatttgttcc ttttaaaatc tcatctacga agcagtagca gcgttctttg
     8221 gaagccactt ggtccagcaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
     8281 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
     8341 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
     8401 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
     8461 ttgactggat taactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
     8521 ccgccctcag taaactctgg tcggcaagtg atcggcatat aggtgcgata attcaaaacg
     8581 gctgctgcaa cttccaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
     8641 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
     8701 acatattcaa aaatcaattc tgcttcactc ccgctcttca ttcgaaatga aaaacctaat
     8761 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct ctttttgaag tggtgtcgtc
     8821 atttttgcta actgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
     8881 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taagccaccg
     8941 attgctagat agattcctag tacttgccct aaaagaagca aacctacacc caccagcgga
     9001 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
     9061 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
     9121 ctttctctca gttgaggatt ttcctgataa aaagcaatta gtttttctaa ctgataatct
     9181 tctccaaaat caaagctacg taaacgctga tacaacgctt ctgaacctac actagaatag
     9241 gtactgttga tcccttcaaa tacttcgaac atatccaagt cataccaagt aagatcgtcg
     9301 atttcactat cccatgaacg gaattttttc tccgtgagcc acgcatcctt caaactttct
     9361 tctttatcaa atctcggttg ataaggaatt tttccccaat tactacgaac tgtttccttt
     9421 actttcaaac gtgaaaaaat gtctaaaaca actaataaaa taacaactgc aataataatt
     9481 aaaacgataa attctctttg gcccatatcc ctctccgttc cttgatttgt tagttttagt
     9541 atagcactgt tgcgctattt gaaaaggggg tacaaaaata actgtcttcg ttacctaatt
     9601 cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag aagatgtctt taaaacgaga
     9661 tacaattttt tgtcattcac ttcaggtaaa ttagagccaa ttaaaccctc cgcaacttga
     9721 cggagatgga gaagatgccc ccgtctaatc tagcggtcat ctctcctccc attccttcca
     9781 ccaatttttt tgacaggtaa agcccgattc cgctagattc cgtatttgtg cggctggtat
     9841 tttcggtatg aaaacgctgg aagacttttt caacagaggt ttgcgctcct ggtttgatag
     9901 gattggccac tgttaatcga atgaatgaat cctcttttga aaatgtcacc tttgcttgtc
     9961 catctgcata acgcaataaa ttctgtatga tattttgcag cactcgcagc aacatgagcg
    10021 gatctgctaa tacagttgta tttatcgagt gttcagcgaa cgctacctcg atatttttct
    10081 cagaaaattg ttcataaaaa gagaaaaata cttcttccac ctctttagaa aaagaaagcg
    10141 tatggagatc catttctttt tgcttcgtct ctattcgagc aagatcataa aactgatccg
    10201 tcatactgag taatcttgca acagattgtt tgatgatcgt taaatacttc tcccgtttct
    10261 ccgtatcctc tacctgttcc agtaattgta cataaccgtt aatagcagtc aaaggagttc
    10321 tgagatcatg agaaaggccg acgatcgata gtctgatgtt ttcttccatt tcttgaaatg
    10381 cttgtccttt ttgttcattt tcatccaccc atgtattcag tgcatctact aggtccatta
    10441 gattcttttc tcgaaattcg actgaaagac gaccaccata ttcttgtcgt tcagaaagag
    10501 tggcgatttt cttagttaat ctcttgattt ccctttgcag gaagaaaata aaacttccca
    10561 taaagataac aacgagaagc aacagccact taatcaaatc taaaccccac tccccacacc
    10621 gtatgaatat acacagcatg tgcctttgct agtttccctc gaagattgga aatatgcata
    10681 ttcactgttt tctcactatc aaaatatggt tcttgccaaa ctgtttcata aagatttgct
    10741 cgcgaaaata cttttttcgg atgttctaaa aaaagcttca gcagatcgaa ttctcttcct
    10801 gtcaaatgga cagccacacc attcacattt acatccctcg actctttgtc cagtaagatt
    10861 tccttgtaat agatgcgttg cgacagatct tttacttgga cagaagtctg atgacgaagt
    10921 tggatgccga ttcgtaacaa cagttcttcg atatcaaatg gtttcgtcac atagtcatcc
    10981 gctcctgctg ccaacaagga aactttatct ctttgatcgc tttttgctga aagaacgatg
    11041 atcggaacat tcgatgtctg tcggatttct gctaacaatg cttcacctga aagccctgga
    11101 agcatgagat ctagaaggat caaatcgaca ggatatgtgc ttaacaataa ttttccttcc
    11161 gtacctgaat aagcatcaag aagtttgtat ctctgttcaa gaacctcctc cagtaaagaa
    11221 tgaacagctt catcgtcttc gataatcaaa atagtttcca gcaggaacac ctacttaatg
    11281 tcagattttt ggaaaataaa cattcctaga gctgatgata ccacgattgt tactagactg
    11341 accagaatca ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg
    11401 agccacgttt ccgtaaacca ctccgttaga aattccaagc ctttgatttt tgcgaaaaca
    11461 ccgggaacga aggccgttcc catgatataa agaaaagaaa gaagcaaact gacactggta
    11521 cttctagtga tgaatgtcaa agccactgga acagataatg tcgctaatat cagcagaagc
    11581 aatacaggaa atacttttag aacataattt ccatagtttt tgattccctc aacttcacca
    11641 aatccgaaag caagtgtgta agcaatcatg gctagccccg taatcaaaac cacgccaagt
    11701 gttgtgatca aaagcgtaac gagaagtttc gacacataat aagtcattcg attccgtcct
    11761 aaagataacg tattgcgaat cgtaccgttt tgaaattctt ccccaatata aaagcacgca
    11821 aatgctgcga aagccagaag aatccatgca gccatttttt ccaccattaa aatccctgtt
    11881 gcaccgttga tgactgtatc cgttgcttgg tttaattcat tgccgtattt tgtatgcata
    11941 taccattcgt tccaagaata aaaaagcgga agtacgacca tcagtcctag tgagacccac
    12001 attttacggc tcttccatac ttttattttt tcagcagtta atagattaaa catgtggatt
    12061 gcctccagtt aattgtaaga aataatcttc cagtttttgc ccaactaggt ggataccatc
    12121 cacaggaact tttgcctcta ccaatgcttg attgatggct gctacttggt caattccttc
    12181 aaaaagctgg ataacacggc tagattggac aaaataatcc ctataaccaa gagagtcgag
    12241 aaccgttact gcttgttccg tcgccgaagt gtcaattttt ataaattgac gagattcttg
    12301 tgccaattct tctttcgata attctttgat caagtgtcct tcatgcaaaa taccgtagtg
    12361 agtggcgatc tgtgacaatt catctagcaa gtggctagaa agcaagacgg tgatgccccg
    12421 ttcttttacc aaacgttgga tgatttctcg catttctgcc atccctgctg gatccatccc
    12481 attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc
    12541 taaacgctgg cgcataccta aagagaaatt ctttgctttc tttttccctg tattttccaa
    12601 tcgcatcatt ttcagcaggt cttcgatttc tctatccgac acaccgccgt tcgctgcttg
    12661 gatacgtagg ttatctcttg cagttaattc tggataaaga gcaggggttt caatcgattg
    12721 acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca aataattcga tcgaaccttc
    12781 atcaatcgag atcaaaccca taatagttct cataaaggta gacttacccg ctccattctc
    12841 cccaatcaaa ccatagatca ttccacgttt gatttcgatc gatactttat ctaatgcttt
    12901 ggcagcaccg tattttttgg ttataccagt tgctttcaag accgtttcat ttccaaaatc
    12961 catacttatc cttctttctt attgataagt gaagtatagt tgacgggtat aaagaaagca
    13021 gccagaaatg tataaagaaa gtgtaaattt tccaattgaa tttcaagaaa aatcagactg
    13081 tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg caccaaacag tgctaatcca
    13141 agtggactgg ccaccatggt aatcgttgtt atcgttgaca tcagacgtcc ttgtatatgc
    13201 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
    13261 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
    13321 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
    13381 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
    13441 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
    13501 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
    13561 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
    13621 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
    13681 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
    13741 gctttcttaa gacctggtta aacaaaactc tattttattg cattttacag gaatgtattt
    13801 catgcgaaat taataagtct ttgtttatag agttttacta gggtataaaa agttgtatgt
    13861 tatcattacc tcaaaacatt acctcagctt ggcgagttgg ttgattgaag aataaatcat
    13921 ttatctagat atctcaataa aaaatgattt ctaagaaact tg
//
LOCUS       DAZKAO010000071        13902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000071 DAZKAO010000000
VERSION     DAZKAO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.071"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..177)
                     /locus_tag="ACG8H8_001767"
     CDS             complement(<1..177)
                     /locus_tag="ACG8H8_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP8986836.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLAS"
     gene            complement(656..1375)
                     /locus_tag="ACG8H8_001768"
     CDS             complement(656..1375)
                     /locus_tag="ACG8H8_001768"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP8986837.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1424..3070)
                     /locus_tag="ACG8H8_001769"
     CDS             complement(1424..3070)
                     /locus_tag="ACG8H8_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP8986838.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3249..3839
                     /locus_tag="ACG8H8_001770"
     CDS             3249..3839
                     /locus_tag="ACG8H8_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8986839.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3931..5436
                     /locus_tag="ACG8H8_001771"
     CDS             3931..5436
                     /locus_tag="ACG8H8_001771"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP8986840.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5520..6872)
                     /gene="celB"
                     /locus_tag="ACG8H8_001772"
     CDS             complement(5520..6872)
                     /gene="celB"
                     /locus_tag="ACG8H8_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP8986841.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6872..7390)
                     /locus_tag="ACG8H8_001773"
     CDS             complement(6872..7390)
                     /locus_tag="ACG8H8_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986842.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7406..7732)
                     /locus_tag="ACG8H8_001774"
     CDS             complement(7406..7732)
                     /locus_tag="ACG8H8_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP8986843.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7745..9724)
                     /locus_tag="ACG8H8_001775"
     CDS             complement(7745..9724)
                     /locus_tag="ACG8H8_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8986844.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9745..10059)
                     /locus_tag="ACG8H8_001776"
     CDS             complement(9745..10059)
                     /locus_tag="ACG8H8_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP8986845.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10227..10520
                     /locus_tag="ACG8H8_001777"
     CDS             10227..10520
                     /locus_tag="ACG8H8_001777"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP8986846.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10598..11710)
                     /locus_tag="ACG8H8_001778"
     CDS             complement(10598..11710)
                     /locus_tag="ACG8H8_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8986847.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11863..12711
                     /locus_tag="ACG8H8_001779"
                     /pseudo
     CDS             11863..12711
                     /locus_tag="ACG8H8_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13064..>13902)
                     /locus_tag="ACG8H8_001780"
     CDS             complement(13064..>13902)
                     /locus_tag="ACG8H8_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8986848.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4336 a         2838 c         2383 g         4345 t
ORIGIN      
        1 gctagccaag aacccatctg ctgaaatatg aaaatttttg aatgctacga cattcgccat
       61 tacaggtatg taaaagaaaa aaatcatcca aagcataccc ggtacagcca tcaggacaag
      121 tgctttataa cgccatagat tcataaaaaa atgattgaac ccctttcgtt ttttcatcta
      181 aaaacccctt tcgattcatt cgcaacgtaa ttatagcgct ttcaataatg agaataaata
      241 aacaaagtat agggaaaatg aaacaaagta taggagaggt tgtgaaatca gcgttttggc
      301 ctgagtatta gagaaaaaaa cgggaaaata gttcctcata ttttgccgtt ttttgagcta
      361 ataccgaggg tcagctgatt gaacaccgtt tatcagaggt tgtgaattca gcgttttggc
      421 ctgagtatta gggagatcgt gtccttacta tctaactctg agtcacaagg aacaatgggg
      481 actgtaacaa aagtcttgtc acagtccctt attccgaaca aacggtgttc cagaagtagc
      541 ttctacggaa ataagccgaa atttcacaaa aaagccatcc gaaagtcaga cggacaagtc
      601 tgcttttcag atggcgctac aactactctg cttattcttt tagctatcca ttcaattatt
      661 tttctctcac ttgttctagc tcttctttgg tcatgggtcg atattgccct ttattcaaat
      721 ctttaggcag ccaaaatttt cccatacgga ttctttttaa atataagact tcttttccta
      781 ctgctttgac cattcgctta acttggtgaa actttccttc atgcaaaatc agacggataa
      841 ttgaagtttc tgtctcttga tctacttgat caatttggag ttttgccggc aatgtctgct
      901 catcgccatc aataatcagt ccttctgcaa ataaacggat atcttcttct gtcattatac
      961 cgctaacctc agctagatat tctttctcca catgtttttt aggtgatagt aactgatggg
     1021 caaacacccc gtcattcgtt aaaatcaaga ggccctctgt gtctttatct aatcggccga
     1081 ctggaaaaag atcttcccga taatcctggt cttccaataa atcgattacg gtttgatcat
     1141 gattatcctg agtagcagaa atgactcctg caggtttatg caaaatgtag taaaaatctt
     1201 tttgatacgt aatgatttct cctaaataag taaccgcatc ttcgaattca tttacttgcg
     1261 ctttatcgct tttcaccact tgtccattga cttcgatcgt tccttttttg attaatcctt
     1321 tgacttcttt gcggctgcca agtcccatat ctgctaaaaa cttatctaaa cgcatgcgtt
     1381 tcccccattt caaaagagca tcaatctagc tcttctgatt cacttatttt atttttaatt
     1441 tatgtcgtaa acgcgctatt cctggtccta gtagtctttc tgccaagcgg attttcaaag
     1501 ctaaataacc atacactaaa gcccctatac ctgctactac aagaatcaac aatagagact
     1561 ggaatttgct atcttcgttt aaaaaagcac caaaaatcat ctttgtgatc cacgaagcca
     1621 aaagcataat caaggtaaga ataaagatca gcaatgtcct gcgccagaca aaacttggtt
     1681 taaagcgggc tacttcatgg attctcttta agatcaaggt acaagacaca gcaaatccga
     1741 tcattgtcgc caataaaggg ccgtatactt caaataaacg aatggctggg tattgaagaa
     1801 ggagtttcac tagaaaccca ataaccaaat aattaattgc cgccttgttt tcaaacattc
     1861 cttgcagcat gttggaaact agcatatata gtcctaggaa gagtcctaca aaacttgctt
     1921 ggatcagtac attacttcct agagaatccg gtgtataaaa taatgtattt agcggatagg
     1981 caagtaacat aacaccaaat gtcgccggaa acataaaaaa tgaaaacaac tgtaaatttc
     2041 cgcttgtcaa ttttgccaga cctactcgat ctttcaacgt aacagcttct gtaatcaacg
     2101 gtaacccagt agacgcgatc gatgttgcca atgcaataac taccatcgtc aacttatctg
     2161 gattggcact aaaaatcgaa aaaagatcca gtaactgtgc attggaatac tcggtggatg
     2221 cagacatgat cttcataaaa gtgaactgat cgactaattt gaagatcgtt acccctgaac
     2281 ccacaataat aaacggaatc gcttctttaa cggtatctaa caacaattcc ttcgttgcaa
     2341 tatttatttt gttttcgctg tagtgaataa acgccgatgt atacgcttga tgtttcttca
     2401 aaaaatacaa aaggacaaga atacttgcta gcataccgat aaatgccgcg aaagttgatt
     2461 gcgtgaccgc cgtcacatag tcaccatcca tgactttcat gatgatgaat gtagccaata
     2521 gcatataaaa aatacgtgcc acctgttcta caatctgcga cagcgcataa ggcatcattt
     2581 cttgattgcc ttgcaaatac cctcgtatca cactcataca aggaaaaacc aatacagcga
     2641 tactcaaaga acgcatcgtt ggaatcagtt cttctccgcc accagaagca tgagctagcc
     2701 aaggagaggc gatgtacatg aacaaagcaa acaacaggcc taacccgccc atcatctgta
     2761 atgcacggat aaataatcgt cgtgaagtgc catattcatt caatgaatta tagcgtgcag
     2821 tttgttttgc aatcgcagca ggtattcctg ctgtagagat cagtagaaac aatgcatata
     2881 tattgtatcc catattgaat aatccattgg cagccttagc attttcgccc atccaagcat
     2941 accagggaat gatatagaca gcgcctagca atcttgaaat gatattactg gcggtcatcc
     3001 aagcagagcc tcgtgccatt ttttcttgcg gtgttaattg atctaatgct ggttttcttt
     3061 gattatccat cggtcagatt ccaactttct acaaaacatt actatcattt taattggtaa
     3121 aacagaaact ggcaatcgat tttatataac ttttataaga acaattgttt ttttgaattc
     3181 ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt ttatggctaa catttatagg
     3241 agatatcaat gacacaagaa ataaaaaaac tacccgttat atttttaggt cttttcttta
     3301 tcacagttgg attgaattgg ttcttgctcc ctcacgatat agcttccgcc ggagtgggcg
     3361 ccatcggaca tttagttgaa acggcctttt ctatccaacg ctcagttact gtttggagca
     3421 tcaatcttat gatgctgctt ctagccgctg tcctattagg aaaatcagta ttttttaaga
     3481 ctgtagcggg aagtctgctt tttcctgttg ttttagagat tgttccaaaa gtggaaatga
     3541 tctcctcaca tttcttttca ctgatttcag gaagtctttt gttcagtctt ggggtttaca
     3601 cattgtacac agtcggggcc tcaaatggcg gtataacgat accgccgatt attttccaca
     3661 aattttttcg gcttccaatg ccgcaaggac ttttacttac caatagtctt atcgtctttt
     3721 tgaattatta tgtttttgga ttgttggaga cactattcgt tctcctatct attacagtca
     3781 gttcattgtt tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa tttgaataaa
     3841 gctggatttt gtcatagcgg aaacaaacga tatattaaaa agagtgtgct ataataaacc
     3901 aagaaattga ataaagcgag ggatcaatca atgagcttct ctttagaaga catccgtcat
     3961 ttactattaa aagaaaatct attaaaagaa tttgtttcaa caagaggctg gcatcttgat
     4021 gtaccagaaa aagctgaatt tcttcagtta agttatgatt caagaaaagc tgatgcttct
     4081 actttgtttt tctgtaaagg aatgaatttc aaagaagaat atttggattc agcaatcgaa
     4141 cagggtattc aatattatgt atcagaacaa ccatacgaaa attcagcagt aggaatcatc
     4201 gtaacggata tccgtaaagc aatggcgctt cttgctatgg ctttttacga ctatccgcaa
     4261 aataaattga aagtgatcgg attcactgga acaaaaggta aaacaactgc tgcgtacttt
     4321 acaaaagcaa tcttggatca cacaacaaat aaaaaaacag cattgctttc tactatgaat
     4381 acaacgttag atggcaaaac atatttcaaa tcccatttga caacgcctga gtcattagat
     4441 ttatatcgta tgatgtcaga agcagttgaa aatggtatga ctcatctagt gatggaagtt
     4501 tcttcacaag cttacaaaac acaacgcgta tacggcttga ctttagatgt cggaatcttc
     4561 ttgaatatct cgcctgacca tatcagtccg atcgaacatc cgacatttga tgactatttc
     4621 tattgcaagc gacaattgat cttgaattca aaaacagttg ttttaaatca tgaaagtgac
     4681 tattttgatt tacttaggga aacagccgat ctattccaga ttccaacgat cacttatggt
     4741 cgttcggaag aaagcaatta tcaagtgatc cgttcagata aaggtactca cggttttact
     4801 ttatcttctc atgaagatcg cttagctatc tgcgatactg cttatgatat tttgctggct
     4861 ggaggattca accaagaaaa tgcagccagt gcgatcattg ccgcagcact aacaggtgcc
     4921 tccagtcaag atgcacaaga cggattaaaa gaagctcgtg taccaggccg aatggatcaa
     4981 ttgatccaat ccaatggagc gcatgtctat gtggactatg cacataatta tctaagcttg
     5041 aaaacattgc tgaaatttgc caaaaatgaa catccagatg gccgtgtaat tgttgtatta
     5101 ggcagcccag gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt gctatcagaa
     5161 acagctgaca tcgccttttt gacggcagat gacccagcat ttgaagatcc gaaaaaaatc
     5221 gcggaagaaa tcaatgaagc aattactaat ccagatttga tcgttcatta tgaaatggat
     5281 cgtcctgaag cgattcgacg cgctttagca gaaagcgctc cgcaagattc agtcgttatt
     5341 gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc ttatgaaggg
     5401 gattatgcaa tcgtgaagcg gttgattgaa gaataatggt atttatatac tagtggtttc
     5461 ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt tcttagaaat
     5521 cattttaact tttcactagc taattctgct gccttttctc tttcttcatt taataattta
     5581 ttatcataga atttgataaa tgggaaccag ataatgaatg cgatgattgc acaaatgact
     5641 gctaacacag cagctttcca atcaccacca ctaccaacaa atgctccgat cccgactggt
     5701 gaaggccacg gcatctgtgc aagcatcggt ttgacgattt ccagccgaat ggcaaaataa
     5761 gcgagtgtag cagaagccat cggagctaag aaaaacggaa ccgctaaata gggattgtag
     5821 acgattggca tcccgaaaat gattggttca ttgatattga ataatcctgg tacaagtgag
     5881 gcttttccta acactttcag ctgatcagat ttcgcgagga atgcaataaa gatgatcaat
     5941 cctaaagtcg cacctgatcc accaacggtt acataagaat tgttgaattc tcctgccaac
     6001 ggataatttg cgccttgtgc gttagctgcc atattagcta atacgatcgg ggtcaagaaa
     6061 gaagtgatga tattcgctcc atggatccct acgatccaca atgcatggat caagaaataa
     6121 atcaccatga caccaagcca tgtattcgtc aactgcgtaa caaacccaaa tgggatagca
     6181 atcattttga agatatccgt tcctagtgca actaataatc cattgatgat catcaccaca
     6241 aacgcaatga caaatgttgg gatcagagca gtgaaagaac gagaaactcc tggcggaaca
     6301 acatctggca tttttatgac ccaatttctc ttgacacaca tacaatacaa ttcgaccgca
     6361 ataatcgaca taataatagc tgtaaagatt cctgaagttc ctaaacgttc gacaaaagag
     6421 cccatcctaa taccatttac tacagtttct gtctcattga tagaagcaat caagctgatt
     6481 ttaccatcag tcatgatcaa ttcagggata cacataaaga atgcaaaaac agatagtaat
     6541 gcccccgtga gcggattaac atttaatttt tgttcatctc gttcaattga cgttaattca
     6601 taagcaaaca caatggcaaa gtaaatagat aaaatcccca tcgtcattgt attgacgatc
     6661 atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa atccttctaa aaatgggaac
     6721 ggtaatggca aaatatttaa aaccaaaaac atcgagccta caatcgtgaa aggaatagaa
     6781 gccatccccg ctgccatgac agcccgaacg attttaagtt gcgcaatttt ccccataggt
     6841 cccatcaggt atttctgcaa aaaatcaaac attagttatt ctcctttcct aaatataacg
     6901 ctttttcata ttgtttaata cggatatact gttggtcttg gttgagtgaa attttcttta
     6961 aacgatactg tataaatgca ctggcaaaaa taccgcttac aacaagaatg aaaacaaata
     7021 actgtcctcc attgttattt gccataaaag gatacaagaa tgtaaatcct gaggtaaaca
     7081 acactggaat cactaataaa ttaaagacac tctggaacaa aaaataccac tttgtccatg
     7141 gaacgttcgt ttggtgtttt cgataaagct ttacttgttc tcctactgca ggcaaaatag
     7201 ctaaaatcag aatcatcggt aaactacttc ctaaactttt agctgataga agcagaatca
     7261 tccattgcat gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt agataacgat
     7321 tgaaatacat gtttttcaaa ctaagttgct ttttttctaa ttcgattttc ctttttactg
     7381 tatcctgcac aatgatcctt cctttctatt tatttttttg atacaaataa ttcatttcct
     7441 gtgcaacttc caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg accatgataa
     7501 tttccatttc tatttttgtc ccgctggcat attctttcag aagttgagtt tgtgactgat
     7561 gcgcatgaag aatctcttca tttgcttgtc gcaacttctc ttctacttct tcaaacatct
     7621 ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg ctatggaaaa
     7681 tgatatcaaa agaagtcact tgtagtgttt cactgtttat tttttcagcc atctgttttc
     7741 ctccttattc gatcagtttg ataaatttac tgctgaaatt ctcaaaaata ggttcagcta
     7801 agatttcctg ctgtactttt agctggtcaa ctaacctaac gattgcttta gtcacgaccg
     7861 taattccttc attttcgaca taagaaggcg aaactaaaaa aacaaaatga atttcttgat
     7921 attgatccca ctgcatccca gaaggaatca atgcaacggc tacctttgtt tcgattccaa
     7981 ctggaacagc gggatgtggg actgccactg tatcactgaa tatgatttgt cccatatttt
     8041 cacgttgctg gatctgcttt cgcatttctc ttatatatcc ttcttcttca tttatgctca
     8101 gcttttgcag caaatcatga agaatttctt cctttttcgg ttcgtacgta taagtcttga
     8161 aaaatttttc agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga gaaaatgctg
     8221 gtatcggtct atctgaggaa tgatagggta tagattctcc aactaccttt cgtattttac
     8281 gaacatcatc ttcattcaaa aaaatactga catgtaaaac tggaattttg aaaaacattg
     8341 tcgaaagatc aatagaagaa ataatcaggt cgattccttt taaagttgtt tcattgatct
     8401 cataatagcc ttttacactt accactgtaa tatttttacc gaattcactg actacgcgat
     8461 tctttaatag ttgggcactg ccgtaacccg ttgcacagat aatcaatgca tgtagtttat
     8521 gcgcagctct ttctttttcc aatgccgcca tgagatgcaa agccaaatag gcccattcat
     8581 cttcattgat ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt tttactgcgt
     8641 caaagacttt gggattttct ttctttatct cctcagtcaa tgggttctca atgcgaatac
     8701 ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag tagtccattg aaaagctgga
     8761 gatcctctac taatgaaaaa tgaaattcct gagacaatct tttcagaact ttcgctaatt
     8821 cctttgatag ttcctgatca ttttgatttt ctttgtgatt cgacttagcc atgagatgta
     8881 atgtcaaata agcaatttct tcttcgggaa acgatacacc tgcgattacc tcaatacgtt
     8941 gaacgatccg ctgcgccact tgatactcta ctcgcttaga aacttcttgt tcgatgccta
     9001 attccttgat ttcaaaccct tctcggagcc gtttgatccc caaagccaag tgtaaagtca
     9061 gattctgtat gataaaatcc gaaactttta acttagcttc tcttgtttca tcgagaatga
     9121 taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct aaatattctt
     9181 tcaatgaatt tccataatgg tttccaaaaa aagtatccat gataaaatgc cgtcgatttc
     9241 tttcttcccc ttcgatccat gtcccttgac catgcttaga aacgattttt agtgaatatg
     9301 gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa actggaacga ctgatataaa
     9361 gctcttctgc caaatcatcc ataaaaagaa tggcatcttc caaaagcaat tggttcaata
     9421 tatacttttg tcgatcttcg gactcataaa atccttgcgg ttctctagca tatccaggta
     9481 ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata tgcagttggt
     9541 agccataacc tggtttagca atgatcttac ctccgttttt ttctactaac tctttgatcc
     9601 gctgcaagta attacggatt gtccgatcag ataataacaa ttcagaagct aaacgttgac
     9661 tggtgacata ctccttcttg tgatcaatca aatatctcaa cagcaatttt tccttctgtt
     9721 tcattatcat ccactcctat tccatcatat gttctcttcg atcacctctg ctaacttctg
     9781 gatgcctgta gggattggga catacgcttg gaaagggata ctaacaacag gctttccaac
     9841 agattctcct aattttttga atttatccaa aaacatcgtc gtttgcgggc tgatcagaaa
     9901 taaatcaaaa tcactattct taatcatatt atcgccttct gtagctgaaa ctgcatctac
     9961 gagtacctct tctctttttt gcttaaagaa atccgttgtc tttgaggcca tcatcgagga
    10021 tgacattcct gccgcacaaa taatcaatgc ttttttcata aatcaatcac tccttttatt
    10081 tttataaacc aagcatatct tgtaagcgtt ttctattcaa aatatatatt tccgtagcat
    10141 tgcggaaaaa atgtgtatca tatcttgaat aataatttct ggcaccgtga tatactagtg
    10201 atagaatttt ttcaggaggg gatttagtga gtatcacagt gaatattttg tatacaagta
    10261 caaatggaca agccagaaaa tttgcagaag aaatggtcga aagaaacatt gtcaaagcga
    10321 ttcgtgcaga agaaggaaac gagaaatatg actattactt tccactagaa gatccagaat
    10381 ctcttttact gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat cacaaatccg
    10441 aaatgatggc tcaaataaac gaactccgta aaaagtataa attaaaaatg aaagtcgaac
    10501 gtttcactga tttaccgtaa aaagatacac atggaatgat agcaatttat catccatgtg
    10561 tatcttcact ttttccttat tttttattcg atctcatcta atgattcaaa ccgtgttttt
    10621 ctcttatttt tcggataagt atttcagtca ccccaatata catgattcct aaacataccc
    10681 caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat aaactggcag
    10741 tgattgccaa agcaccaaaa gaattaaaaa ctgttttata tctataggta gaacaaacac
    10801 ctaaagctag accacctaat ataccgaccc agttaaaagg aataaattgg ctgataccaa
    10861 taaataaaac acatccaatc aatgagccaa tgattcggtc tactgctcgt tctttgaaac
    10921 caatcgacat caatccatag ctagatacaa tagaagaaaa tgcaaaccct gcccacatca
    10981 atcgctgaaa agggacatac tctcctgcaa cgatcaaaag actaatgcca aatgcataga
    11041 tgagcatcca aattttttcc tgcgaataca tgcctttcaa aaaaatctct ttaaacaagc
    11101 ttttgtcttt gttcttttct cgatgttttc tgtaaaggag aactgaaaac caacaaaaaa
    11161 acaaaaataa taagctactt gtttgtgtaa agaaattctt gttcaacgaa tctttaggta
    11221 acgaataaac aataaagaga tatgaaaagc cgtacaaaga agcattcccc attttgggat
    11281 cactagcggt tgccattaat atgctgctta gtaaaataaa atgaatcaac atgcccataa
    11341 ctgaccattt gatcagctgg acatacggtg ctactaatag tgagaacatg acgattgcta
    11401 aacctatgat cgaatgggaa atcttataac caaagtcaac aaaacgaata ctcatcaaca
    11461 tacagaataa aaccacagcg tatggtttat gcttcgattc aaataatgtt gtaattataa
    11521 tgataaaaca aatggcaaac aatacgatga ataatgatcg taaaaacaat gaacaaatat
    11581 acatgcactt ttctttttta gaagctgctt ctcgtatttt ttgttttaaa ataaacggat
    11641 ctagttgtag agactgataa aaatccacct gttgtcgtcc tttcttttta cttttatttt
    11701 ttacagtcat ctatgacagg ttgtaaatac tatggaacat catatggcgt tgtcattttc
    11761 ttgtcaacta tttttcctat tcatgttttt ataaaaaaac atatcgtttt cttgtatgat
    11821 taaatcacca caaaataaac aactgagaga acgatatgct ttatgaatgg ttctatcaaa
    11881 aaaattctga gatcaataga caaaaatttg atgattacag aggtctctta cgaaacctta
    11941 cagaagaaaa aaacattgat cgttgctgca gttctcttcc ctgctccatg tgtctgtaga
    12001 aattgtggtt ctgctgtggt agatggaaat aggaaattaa tggtggtgaa aaacaagaag
    12061 aaggaaacca ttgtccactt tgaataatat aatcatatgc caatggttat acgcctaaaa
    12121 aagcagcgct atacctgtaa aaactgtcga agccattgga acgctcaaag ttattttatc
    12181 cgtccaaggc actcgatttc aaatcatgtc agacataaaa tcacttcttt actgactgaa
    12241 aaagtgtcgt tattttttat ttcgaaaagt tgttaggtat ctttgacaac cgttattcgc
    12301 acattgaaag agttgagaag ctatttacca aagtcttcca aaagaattct gtcttgagtg
    12361 ttgagggttg tgaatttcgt tctcacgttt ccgttgagga taaactgagt tttatttccg
    12421 cggatggcga gacagaaaaa ttaatcgaga aaatttacct acacaaaaaa ttcctcgatt
    12481 aacaaactat ttccttagct gcactaatcc taaagaaata gaattcttag ttacagacat
    12541 gaacgccacc tactttcagc tcaccaaaca tgttctacca aatgcgaaat tggtgattga
    12601 tcgattactt ggtttttcca cgcctttcaa ggaggcgtaa cctttattca cgagttggtt
    12661 gaagctttcc gagacaaaag acccgacttg tttttctcct tattgacaaa gagccattaa
    12721 aactagagaa aaaattacaa ctgatcgctg attatttgta gaaagaaaat caaagtatta
    12781 cccaataaac ttgagtcatt aggcaaaaaa cactgcgaaa ctcaagaatg attttcgcag
    12841 tgtttttgta tattttttat taactagtat tactacaaag gtttattttt cacttctcaa
    12901 cgcagcacaa aattgtcttt acttatttac tggttgggtt caaccttctc tactccgata
    12961 ctaatatttg tttgtatacc ccttctaaaa tcggtagttt ccattcaatt gatgggtact
    13021 agttgatttt aaaaactatg cttattcaat tttttatttt agctcatata tttcgtttct
    13081 ttctaaattt tatactgatg aaaacaagta gcacacccaa tattgacaat ataaactggc
    13141 taatgatggt ttctccagtt tttggaagat gcttatttcc actgcttgtt ggattgtttc
    13201 ctcttatatc tgagctatca gcaacttttc tattctcttt tgttaaaaag atcgccactt
    13261 cttgttcttt ttcgatcttg aaagttactg gtgtcgcatc caattcatac cccgttggcg
    13321 cttgagtttc taccagctga tatgctcctg gtgctaagcc atctatcgcc agttttccat
    13381 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
    13441 cagctccttg caggacttct ccgctttggt catcgatctt tgtcaacacg acaccgcctg
    13501 gtgtaagtcg attttctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
    13561 actcgattgg tgtcgcatcc aattcatacc ccgttggcgc ttgggtttct accagctgat
    13621 acactcctgg tgctaagccg tcaatcgcca gttttccatc tgctcctgtg gttagacctg
    13681 actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttccc
    13741 cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcgg tttgctttcg
    13801 tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgatcggt gtcgcatcta
    13861 attcataccc cgttggcgct tgagtttcta ccagctgata tg
//
LOCUS       DAZKAO010000072        13829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000072 DAZKAO010000000
VERSION     DAZKAO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.072"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..2253
                     /gene="acm"
                     /locus_tag="ACG8H8_001781"
     CDS             88..2253
                     /gene="acm"
                     /locus_tag="ACG8H8_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP8986849.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2525..3010)
                     /locus_tag="ACG8H8_001782"
     CDS             complement(2525..3010)
                     /locus_tag="ACG8H8_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP8986850.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3100..3445
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3490..4131
                     /locus_tag="ACG8H8_001783"
     CDS             3490..4131
                     /locus_tag="ACG8H8_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8986851.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4150..4785
                     /locus_tag="ACG8H8_001784"
     CDS             4150..4785
                     /locus_tag="ACG8H8_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8986852.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4803..5618
                     /locus_tag="ACG8H8_001785"
     CDS             4803..5618
                     /locus_tag="ACG8H8_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8986853.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5937..7373
                     /locus_tag="ACG8H8_001786"
     CDS             5937..7373
                     /locus_tag="ACG8H8_001786"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8986854.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7562..10009
                     /locus_tag="ACG8H8_001787"
     CDS             7562..10009
                     /locus_tag="ACG8H8_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP8986855.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10239..11306
                     /gene="rlmN"
                     /locus_tag="ACG8H8_001788"
     CDS             10239..11306
                     /gene="rlmN"
                     /locus_tag="ACG8H8_001788"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP8986856.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11296..11502
                     /locus_tag="ACG8H8_001789"
     CDS             11296..11502
                     /locus_tag="ACG8H8_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986857.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11499..12602)
                     /locus_tag="ACG8H8_001790"
     CDS             complement(11499..12602)
                     /locus_tag="ACG8H8_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8986858.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13155..13703
                     /gene="aac(6')"
                     /locus_tag="ACG8H8_001791"
     CDS             13155..13703
                     /gene="aac(6')"
                     /locus_tag="ACG8H8_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP8986859.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4734 a         2298 c         2935 g         3862 t
ORIGIN      
        1 aatgagtggg ggaggaattg aagaaaggat ttttgagaga tgatatagta ggttttattt
       61 ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg
      121 ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc
      181 agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga
      241 ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat
      301 tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa
      361 atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg
      421 caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt
      481 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
      541 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      601 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      661 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      721 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
      781 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
      841 gctacattta atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt
      901 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
      961 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
     1021 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
     1081 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
     1141 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
     1201 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
     1261 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
     1321 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
     1381 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
     1441 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1501 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1561 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1621 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1681 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1741 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     1801 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     1861 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     1921 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     1981 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     2041 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     2101 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     2161 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     2221 gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata
     2281 tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat
     2341 atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg
     2401 tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg
     2461 atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct
     2521 atcattacaa attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt
     2581 cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt
     2641 gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg
     2701 ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa
     2761 caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt
     2821 catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt
     2881 ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat
     2941 cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg
     3001 cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca
     3061 aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga
     3121 gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt
     3181 gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt
     3241 acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa
     3301 aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt
     3361 tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg
     3421 gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag
     3481 gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca
     3541 acattgaaat tatttttatt taccttgatt ggctcattac cattagggat tgttttagca
     3601 ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt
     3661 atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt
     3721 gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct
     3781 tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca
     3841 gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg
     3901 acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc
     3961 ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc
     4021 gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt
     4081 ctaacaatcg taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga
     4141 ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc
     4201 aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt
     4261 ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa
     4321 ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta
     4381 ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga aaacattacc
     4441 ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa
     4501 ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca gctttctggc
     4561 ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg ggtattggct
     4621 tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc
     4681 ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca
     4741 gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag
     4801 agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct
     4861 gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg
     4921 ttataggttt agacgataca tttgttccta tgggatttag agataaagac gggaagctca
     4981 caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact
     5041 ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga
     5101 tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt
     5161 atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac
     5221 aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga
     5281 aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt
     5341 ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag
     5401 tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag
     5461 gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga
     5521 aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg
     5581 ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa
     5641 gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc
     5701 ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg
     5761 tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac
     5821 tcgaaactaa gcgacttttt cacaacctcc tttacatatt tttattttgt tgtggaaggg
     5881 gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag agatgaatgg
     5941 aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct tctgcagcgt
     6001 atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg gatcgatttg
     6061 tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta gaccactatc
     6121 atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct taccgcttct
     6181 ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa gctgggctcg
     6241 ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct atgttgactt
     6301 tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg gaatcacgtc
     6361 agatcattga agatttcact cattatgcag aaacattatt tgaggcattc cgcgggaaag
     6421 tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt tatttgttag
     6481 cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac catattgcta
     6541 atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg
     6601 gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa aatgttctgg
     6661 ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat atgtggggaa
     6721 aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca acgattgaag
     6781 ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc aattactatc
     6841 aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa atgaatacga
     6901 caggaaaaaa aggttcatca gaagaaactg gagttcctgg aatgtataaa aaagttgaga
     6961 atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga ttaaggatcg
     7021 gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa aatggcttag
     7081 gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga atcaattact
     7141 tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa gtcctaggct
     7201 attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa aagcgatatg
     7261 gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga tataagaaag
     7321 acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt tagttcacat
     7381 acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa aatcatttag
     7441 aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac
     7501 aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg agggataata
     7561 aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca
     7621 aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga ttttatttgc
     7681 tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg gctttcggat
     7741 cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag gattttggat
     7801 tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg gcggcatact
     7861 cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac tccatactgg
     7921 ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa aacaaagtca
     7981 agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt ttacttactt
     8041 cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag gaggagcatt
     8101 cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa ttggagaacg
     8161 agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag cggcaaagga
     8221 agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg caaaagaagc
     8281 tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa
     8341 tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc
     8401 aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac
     8461 accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac
     8521 ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt
     8581 tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa
     8641 aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa
     8701 agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt
     8761 aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg
     8821 gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat
     8881 cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact
     8941 agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat
     9001 gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat
     9061 tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc
     9121 gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac
     9181 gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata
     9241 tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca
     9301 gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga
     9361 tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt
     9421 agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt
     9481 ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc
     9541 aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg
     9601 aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt
     9661 catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca
     9721 ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc
     9781 acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat
     9841 ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga
     9901 actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat
     9961 tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga
    10021 tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa
    10081 aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga
    10141 gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata
    10201 gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg
    10261 gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag
    10321 ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga
    10381 atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac
    10441 agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata
    10501 aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga
    10561 cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc
    10621 gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg
    10681 agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg
    10741 ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa
    10801 ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg
    10861 ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc
    10921 gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg
    10981 atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag
    11041 taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat
    11101 tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca
    11161 gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg
    11221 tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc
    11281 aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa
    11341 ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg
    11401 aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat
    11461 ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct
    11521 tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt
    11581 tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag
    11641 attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg
    11701 ttctttttca taaacacata gattttatca aacaaatcga actgctgatc catcatcaat
    11761 aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga
    11821 acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa
    11881 acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag
    11941 ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg
    12001 cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca
    12061 gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta
    12121 tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga
    12181 aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc
    12241 gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg
    12301 acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg
    12361 acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag
    12421 agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt
    12481 gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca
    12541 tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc
    12601 atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg
    12661 actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg
    12721 catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga
    12781 aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt
    12841 gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca
    12901 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
    12961 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
    13021 aattttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
    13081 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
    13141 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
    13201 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
    13261 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
    13321 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
    13381 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
    13441 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
    13501 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
    13561 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
    13621 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
    13681 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
    13741 aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc
    13801 gaagaagcta cttttggaac accgtgtac
//
LOCUS       DAZKAO010000073        13752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000073 DAZKAO010000000
VERSION     DAZKAO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.073"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1580
                     /locus_tag="ACG8H8_001792"
     CDS             153..1580
                     /locus_tag="ACG8H8_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8986860.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1672..2037)
                     /locus_tag="ACG8H8_001793"
     CDS             complement(1672..2037)
                     /locus_tag="ACG8H8_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP8986861.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2034..2399)
                     /locus_tag="ACG8H8_001794"
     CDS             complement(2034..2399)
                     /locus_tag="ACG8H8_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP8986862.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2591..2839)
                     /locus_tag="ACG8H8_001795"
     CDS             complement(2591..2839)
                     /locus_tag="ACG8H8_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986863.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2838..2966
                     /locus_tag="ACG8H8_001796"
     CDS             2838..2966
                     /locus_tag="ACG8H8_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP8986864.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3023..4717
                     /locus_tag="ACG8H8_001797"
     CDS             3023..4717
                     /locus_tag="ACG8H8_001797"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP8986865.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4784..5113
                     /locus_tag="ACG8H8_001798"
     CDS             4784..5113
                     /locus_tag="ACG8H8_001798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986866.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5491..6624
                     /locus_tag="ACG8H8_001799"
     CDS             5491..6624
                     /locus_tag="ACG8H8_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP8986867.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6655..6975
                     /locus_tag="ACG8H8_001800"
     CDS             6655..6975
                     /locus_tag="ACG8H8_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP8986868.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(7004..7468)
                     /locus_tag="ACG8H8_001801"
     CDS             complement(7004..7468)
                     /locus_tag="ACG8H8_001801"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8986869.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7599..8852)
                     /locus_tag="ACG8H8_001802"
     CDS             complement(7599..8852)
                     /locus_tag="ACG8H8_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8986870.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8881..9198)
                     /locus_tag="ACG8H8_001803"
     CDS             complement(8881..9198)
                     /locus_tag="ACG8H8_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8986871.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9224..9544)
                     /locus_tag="ACG8H8_001804"
     CDS             complement(9224..9544)
                     /locus_tag="ACG8H8_001804"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8986872.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(10008..11423)
                     /locus_tag="ACG8H8_001805"
     CDS             complement(10008..11423)
                     /locus_tag="ACG8H8_001805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8986873.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11440..12756)
                     /locus_tag="ACG8H8_001806"
     CDS             complement(11440..12756)
                     /locus_tag="ACG8H8_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8986874.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12906..13604)
                     /locus_tag="ACG8H8_001807"
     CDS             complement(12906..13604)
                     /locus_tag="ACG8H8_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP8986875.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4369 a         2790 c         2361 g         4232 t
ORIGIN      
        1 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
       61 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
      121 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      181 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      241 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      301 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      361 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      421 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      481 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      541 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      601 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      661 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      721 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      781 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      841 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      901 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      961 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
     1021 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1081 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1141 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1201 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1261 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1321 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1381 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1441 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1501 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1561 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1621 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1681 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1741 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1801 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1861 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1921 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1981 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     2041 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2101 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2161 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2221 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2281 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2341 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2401 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2461 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2521 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2581 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2641 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2701 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2761 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2821 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2881 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2941 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     3001 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     3061 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     3121 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3181 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3241 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3301 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3361 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3421 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3481 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3541 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3601 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3661 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3721 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3781 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3841 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3901 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3961 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     4021 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     4081 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     4141 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4201 tatgtctatc gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4261 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4321 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4381 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4441 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4501 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4561 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4621 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4681 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4741 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4801 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt gtgaaaaagc
     4861 tgccctgcat caagtaataa gaacacccaa gctaaaactg cttcttatgt ttttgacttg
     4921 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag gttatgaaaa
     4981 agctgccctg catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac
     5041 ttggttagac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     5101 aaaaggcgtt tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt
     5161 gcataaattt gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta
     5221 tgtctgtttc tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca
     5281 tcatctaacg gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac
     5341 ctctccgaac acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca
     5401 ttatcaaaaa ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac
     5461 aattttatga atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag
     5521 aaaatggaaa tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc
     5581 cgtatcacag caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca
     5641 gttttggaaa aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga
     5701 tctcaagttc aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt
     5761 ggtcattgtc atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat
     5821 cagttatatg gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa
     5881 gcggaatatc taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat
     5941 cttcctgatg aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt
     6001 gaaaacgcag gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt
     6061 ctattcgctc aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca
     6121 gtacagcacc gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa
     6181 gacacggcac aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt
     6241 atcgattgtg taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta
     6301 agcttacaaa gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt
     6361 ggaacagtcc aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa
     6421 ttttttatgc gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca
     6481 aaaatttacg caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg
     6541 ctgccactat cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat
     6601 ataaaagttg ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc
     6661 aaaatcaaac gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga
     6721 ggacgtcgct tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat
     6781 ttattaaccg ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat
     6841 cgatatggca cctattttta tttggctggt gctattttct tgacgattcg tcctattctc
     6901 agaatcgtaa ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa
     6961 aaaaaagagg catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac
     7021 tttgatacag acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact
     7081 cgtatcgttc ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt
     7141 gatgcatttc ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt
     7201 ccggttcaac aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt
     7261 tttcaatgac gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt
     7321 gaggaatact ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag
     7381 gaactcccca ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc
     7441 ttaatttgat ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta
     7501 caaaaattat acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa
     7561 aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt
     7621 cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc
     7681 aaattgacag cggctagtac accacctgca atactttttg ttgccaatac accgccaata
     7741 attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc
     7801 gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc
     7861 gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc
     7921 gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata
     7981 gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa
     8041 ggtttgttca cgataaacgg tgcgctatgc ccagctgcga ttgcattaac gtttgcttca
     8101 atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca
     8161 aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca
     8221 agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg
     8281 aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt
     8341 aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat
     8401 ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca
     8461 ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa
     8521 gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc
     8581 aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat
     8641 cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt
     8701 aagttattga tcattactgc taatgctcct aagatcatta acggcatact caccataaat
     8761 gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa
     8821 tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga
     8881 ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat
     8941 agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc
     9001 ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc
     9061 tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc
     9121 ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag
     9181 atttttttga tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt
     9241 tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta
     9301 catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta
     9361 cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg
     9421 aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt
     9481 ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt
     9541 ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac
     9601 tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac
     9661 acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt
     9721 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
     9781 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
     9841 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
     9901 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
     9961 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
    10021 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
    10081 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
    10141 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
    10201 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
    10261 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
    10321 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
    10381 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
    10441 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
    10501 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
    10561 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
    10621 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
    10681 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
    10741 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
    10801 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
    10861 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
    10921 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
    10981 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
    11041 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
    11101 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
    11161 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
    11221 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
    11281 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
    11341 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
    11401 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
    11461 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
    11521 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
    11581 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
    11641 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
    11701 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
    11761 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
    11821 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
    11881 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
    11941 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
    12001 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
    12061 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
    12121 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
    12181 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
    12241 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
    12301 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
    12361 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
    12421 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
    12481 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
    12541 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
    12601 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
    12661 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
    12721 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
    12781 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
    12841 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
    12901 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
    12961 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
    13021 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
    13081 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
    13141 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
    13201 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
    13261 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
    13321 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
    13381 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
    13441 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
    13501 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
    13561 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
    13621 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
    13681 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
    13741 aagactagga ta
//
LOCUS       DAZKAO010000074        13742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000074 DAZKAO010000000
VERSION     DAZKAO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.074"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..360
                     /gene="yaaA"
                     /locus_tag="ACG8H8_001808"
     CDS             118..360
                     /gene="yaaA"
                     /locus_tag="ACG8H8_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP8986876.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            347..1471
                     /gene="recF"
                     /locus_tag="ACG8H8_001809"
     CDS             347..1471
                     /gene="recF"
                     /locus_tag="ACG8H8_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP8986877.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1468..3414
                     /gene="gyrB"
                     /locus_tag="ACG8H8_001810"
     CDS             1468..3414
                     /gene="gyrB"
                     /locus_tag="ACG8H8_001810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP8986878.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3434..5905
                     /gene="gyrA"
                     /locus_tag="ACG8H8_001811"
     CDS             3434..5905
                     /gene="gyrA"
                     /locus_tag="ACG8H8_001811"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP8986879.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6141..6440
                     /gene="rpsF"
                     /locus_tag="ACG8H8_001812"
     CDS             6141..6440
                     /gene="rpsF"
                     /locus_tag="ACG8H8_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP8986880.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6490..7002
                     /gene="ssb"
                     /locus_tag="ACG8H8_001813"
     CDS             6490..7002
                     /gene="ssb"
                     /locus_tag="ACG8H8_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP8986881.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7028..7264
                     /gene="rpsR"
                     /locus_tag="ACG8H8_001814"
     CDS             7028..7264
                     /gene="rpsR"
                     /locus_tag="ACG8H8_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP8986882.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7407..9380
                     /locus_tag="ACG8H8_001815"
     CDS             7407..9380
                     /locus_tag="ACG8H8_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP8986883.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9386..9838
                     /gene="rplI"
                     /locus_tag="ACG8H8_001816"
     CDS             9386..9838
                     /gene="rplI"
                     /locus_tag="ACG8H8_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP8986884.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10112..11479
                     /gene="dnaB"
                     /locus_tag="ACG8H8_001817"
     CDS             10112..11479
                     /gene="dnaB"
                     /locus_tag="ACG8H8_001817"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP8986885.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12259..13551
                     /locus_tag="ACG8H8_001818"
     CDS             12259..13551
                     /locus_tag="ACG8H8_001818"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP8986886.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4579 a         2477 c         3022 g         3664 t
ORIGIN      
        1 atcacctttt ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat
       61 tgcgaaaaaa aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg
      121 aaacaacaag tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc
      181 acagttatcg gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc
      241 gatggcgaac cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt
      301 ccagaagtag gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa
      361 gctgtattta aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct
      421 cgtcatcttt ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt
      481 attagctatg acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga
      541 cgatcaggcg atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat
      601 cttattatct aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaaa aaaagttaag
      661 tacttacgtg ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa
      721 aggcagcccg cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat
      781 ctatctttat gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtatttaaa
      841 gcaattgaat gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact
      901 agcagcgttt ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt
      961 ttgggctaat tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta
     1021 tctcaccgca gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct
     1081 cgcacttttg aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc
     1141 tcatcgtgac gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca
     1201 aggacagcaa agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga
     1261 agagacgggg gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgatag
     1321 tcgtcaactc catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac
     1381 gacacttgac catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca
     1441 agggaaacta aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct
     1501 aaagagtatg atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga
     1561 cctggcatgt atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt
     1621 gtcgataatt cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt
     1681 gaaaaagaca acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag
     1741 gcaaaaactg gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa
     1801 ttcggcggtg gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgtt
     1861 aacgcgcttt ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa
     1921 gaatatcgtc gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat
     1981 ggaacaacgg ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat
     2041 ttttcaaaat tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata
     2101 tcgattgaag acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg
     2161 attaaaagtt atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta
     2221 tatttggaag gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc
     2281 taccattcca atatcatgag ttttgccaac aatatccata cgtatgaagg cggaacacat
     2341 gaatcaggat tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag
     2401 ctgatgaaag aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct
     2461 gttatttcta tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc
     2521 aattccgaag tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg
     2581 ctggaaaacc caagtgtggg ccgtcaaatt gttgaaaaag gattactagc ttccagagct
     2641 cgtctagcag cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt
     2701 aatctgccag gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc
     2761 atcgtcgaag gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa
     2821 gccatcttgc ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc
     2881 ttggcaaatg aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat
     2941 tttgatgtat cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac
     3001 ggcgcgcata tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt
     3061 gaagctggat acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat
     3121 attacgtatg tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca
     3181 cttcctgcct caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat
     3241 catcaactat gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg
     3301 gacgatgcta ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc
     3361 cgtcgtgcat tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga
     3421 aggaggaaaa agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag
     3481 cgaaatgaaa gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc
     3541 tgatgtcaga gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt
     3601 aggtgtaaca ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg
     3661 taaataccat cctcatggag actatgcgat ctatgagtca atggtacgga tggcacagcc
     3721 attcagctac cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga
     3781 cggtgccgcc gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct
     3841 gcgtgatatc aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga
     3901 accagttgta ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc
     3961 cgtaggtatg gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga
     4021 tttgctgatg gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc
     4081 agattttcca acaggtggtt tggttatggg aaaatcagga atccgaagag cgtatgaaac
     4141 aggtaagggt tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa
     4201 agaacggatc atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg
     4261 gatttctgaa ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc
     4321 ttcccgtgaa ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catctgttat
     4381 cttgaataac ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc
     4441 gatcgaaaaa ggtgtgccga aagttttgag cttgaaacaa attcttgaaa actatatcga
     4501 acatcagcgc gaagtcatca caagacggac agtctttgaa aaagaaaaag cagaagcaag
     4561 ggcacacatt ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat
     4621 tatccgcggg tctaaatcag atgacgaagc aaaagcgacg atgatcgaac ggttcgattt
     4681 atccgatcgc cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga
     4741 acgggaaaaa atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga
     4801 cattctggca agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa
     4861 tcaacgattt ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga
     4921 agatgaagat ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat
     4981 caaacgtttg gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg
     5041 aatgggtatc cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac
     5101 attgttattc tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga
     5161 atacgggcgc acagccaaag gaataccaat tatcaattta ctaggtatcg attcgagtga
     5221 aagcatccag gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt
     5281 tacgacgcgt caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag
     5341 taatggtctg atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac
     5401 agatggtcaa tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga
     5461 agaagcagtc cgcgatatgg gacgtacagc ttccggggtt agaggaatac gcctgcgtga
     5521 ggaagattat gtcgttgggg cagccttaat gaaggaagat caagaagtat tagtgatcac
     5581 agaaaaaggt tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg
     5641 taaagggatc aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac
     5701 tgtttctgga gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa
     5761 cgtatcgact gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga
     5821 agctgatacg aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt
     5881 tgaagaacaa ggatcagaag aatagccatc aagactaatt gtgtaagcta gcaagaatga
     5941 ccgattaaaa aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa
     6001 tattcgaaac tgttcattct gtattgcttt tgaccaaaat agagtgtata atcagagctt
     6061 gtgagtagtc taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt
     6121 ccacagggag gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc
     6181 caaacattga tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag
     6241 ataacggagc agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa
     6301 tgaacggtta ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa
     6361 atgctgtcaa tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga
     6421 ttgttaaaga agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag
     6481 atgactattt tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg
     6541 cgttacacag caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt
     6601 acaaaccaaa gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca
     6661 gcagaaactt tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc
     6721 caaactcgtt cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct
     6781 gacaacttcc aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga
     6841 ttccaaagtt ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca
     6901 tctcaatcat ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca
     6961 agttcaacga tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg
     7021 aaatagaatg gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc
     7081 tgctaaccat attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc
     7141 agaacgcggt aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa
     7201 aattactgtt gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga
     7261 ctaataaatc ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt
     7321 tctgtaaaaa taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca
     7381 aattagtcat atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac
     7441 ctctcttcct tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc
     7501 gttttgttag cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga
     7561 aaatgaaacg ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag
     7621 ctgaaaccaa tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc
     7681 catcaagcca aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg
     7741 gattaacaga ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc
     7801 aagtctatct agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt
     7861 attttaatga tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg
     7921 tgattggtat tttatctatt gataactatg cagatgccat tgataaattg gatgacaaag
     7981 agatttctta tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc
     8041 atgtcttttt caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc
     8101 tgaaaaagat gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa
     8161 aacagagctc gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg
     8221 aaatcggtgg agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag
     8281 ttgtcgtgaa agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta
     8341 agaatgtcaa acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt
     8401 ttgatgaaac aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag
     8461 gctctgcttt tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta
     8521 tcaacgagaa cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc
     8581 ctgaattaga aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg
     8641 acctcctcgt gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg
     8701 agcaatttga taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta
     8761 aacctctgct gacttatatc gaatcttcag cttcttcagc ttcagagcta gtttctgagc
     8821 tgatccaata ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc
     8881 ttgcagggat ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg
     8941 acgttgccag ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga
     9001 gctcggattt agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga
     9061 cttctgatat tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag
     9121 caaaaacagc agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa
     9181 agcggacaga cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa
     9241 tcatcatgga atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa
     9301 atacgacagt tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc
     9361 aaatatatga taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa
     9421 aggtaaaaaa ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa
     9481 aaataacttg gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa
     9541 agcacaagaa aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt
     9601 ggaaaaagaa gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt
     9661 tggttcgatc ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt
     9721 agataaacga aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc
     9781 tgttaagctt catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca
     9841 aatcttgata gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa
     9901 tttatctgag aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga
     9961 tttcggtttc ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag
    10021 aactattttt ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa
    10081 atgaagcaat tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc
    10141 accgcaaaat attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac
    10201 gatcattgat gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat
    10261 tatttttgag acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt
    10321 caaagaccgg ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc
    10381 agacttggca ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga
    10441 agaaaaatca ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt
    10501 cgagcaagga gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt
    10561 ttcggaaaaa agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat
    10621 tgaaaatatc gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta
    10681 tcaaggtctg gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc
    10741 acgtccagcg gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa
    10801 aacagatcgt tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg
    10861 gatgctttgt gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga
    10921 agaagaatgg tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat
    10981 cgatgataca ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca
    11041 agaaaaaggc aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg
    11101 taaagagaac cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa
    11161 agagctgaaa gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca
    11221 agacaaacga ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga
    11281 catcgtagcc ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga
    11341 tggaaacgag ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg
    11401 tgcacgagga acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc
    11461 gccaagagaa gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt
    11521 aagaagatgc aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca
    11581 aagggattgt gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca
    11641 gctgaccctt ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt
    11701 ttctctaata ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct
    11761 cctgccaact cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa
    11821 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt
    11881 caaaagcttg catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt
    11941 gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg
    12001 ttccaaaagt agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt
    12061 ttcgtacatt ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat
    12121 aaccattcta aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat
    12181 cattcgattt tatttgaaat atgtatctat tattgataca atggttcagg attaaataat
    12241 atagaacgag gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa
    12301 gggaaaggaa aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa
    12361 ggtggcgaca atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg
    12421 attccttctg ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc
    12481 aatcctaaat cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt
    12541 aatttgcgta tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa
    12601 ttacaagaag atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct
    12661 gcctatatgg ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa
    12721 atttttgaag aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg
    12781 tttgattcag aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa
    12841 caaatcaaac aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc
    12901 aaacgcgtgt tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat
    12961 ccttttgtta cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg
    13021 ccttcaaaaa tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat
    13081 ggaccatttc caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga
    13141 gaatacggta cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg
    13201 cgccattcta aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta
    13261 agtggattgg aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac
    13321 cactatccag caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca
    13381 ggctggtcag aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt
    13441 aactatgtac gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga
    13501 cctgatagaa cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa
    13561 cgacgactga atcaggaagt tcttaccaga gcgagtcgaa gtgtttatct atacgataat
    13621 aataacttga agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg
    13681 aacactgttt attcagaata agggactgtg acaaaaaccg tgtcacagtc cctttttccg
    13741 aa
//
LOCUS       DAZKAO010000075        13702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000075 DAZKAO010000000
VERSION     DAZKAO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.075"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..901)
                     /locus_tag="ACG8H8_001819"
     CDS             complement(<1..901)
                     /locus_tag="ACG8H8_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP8986887.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="ACG8H8_001820"
     CDS             1184..2152
                     /locus_tag="ACG8H8_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8986888.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="ACG8H8_001821"
     CDS             2265..2543
                     /locus_tag="ACG8H8_001821"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8986889.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="ACG8H8_001822"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="ACG8H8_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP8986890.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3000..3755
                     /locus_tag="ACG8H8_001823"
     CDS             3000..3755
                     /locus_tag="ACG8H8_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8986891.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8H8_001824"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8H8_001824"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP8986892.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8H8_001825"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8H8_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP8986893.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8H8_001826"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8H8_001826"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP8986894.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8H8_001827"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8H8_001827"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP8986895.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8H8_001828"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8H8_001828"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP8986896.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="ACG8H8_001829"
     CDS             8492..9034
                     /locus_tag="ACG8H8_001829"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP8986897.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8H8_001830"
     CDS             9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8H8_001830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP8986898.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="ACG8H8_001831"
     CDS             10627..11529
                     /locus_tag="ACG8H8_001831"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP8986899.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8H8_001832"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8H8_001832"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP8986900.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="ACG8H8_001833"
     CDS             12972..13394
                     /locus_tag="ACG8H8_001833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP8986901.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3733 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatttggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     9901 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
    13561 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 aataagggac tgcgacaaga ct
//
LOCUS       DAZKAO010000076        13439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000076 DAZKAO010000000
VERSION     DAZKAO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.076"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..641)
                     /locus_tag="ACG8H8_001834"
     CDS             complement(99..641)
                     /locus_tag="ACG8H8_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP8986902.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            776..1165
                     /locus_tag="ACG8H8_001835"
     CDS             776..1165
                     /locus_tag="ACG8H8_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986903.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1169..1933
                     /locus_tag="ACG8H8_001836"
     CDS             1169..1933
                     /locus_tag="ACG8H8_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8986904.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2062..3009
                     /locus_tag="ACG8H8_001837"
     CDS             2062..3009
                     /locus_tag="ACG8H8_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8986905.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3139..3879
                     /locus_tag="ACG8H8_001838"
     CDS             3139..3879
                     /locus_tag="ACG8H8_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986906.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4170..4838)
                     /locus_tag="ACG8H8_001839"
     CDS             complement(4170..4838)
                     /locus_tag="ACG8H8_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8986907.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4972..5259
                     /locus_tag="ACG8H8_001840"
     CDS             4972..5259
                     /locus_tag="ACG8H8_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986908.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5675..7213
                     /locus_tag="ACG8H8_001841"
     CDS             5675..7213
                     /locus_tag="ACG8H8_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP8986909.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7210..8175
                     /locus_tag="ACG8H8_001842"
     CDS             7210..8175
                     /locus_tag="ACG8H8_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986910.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8162..9484
                     /locus_tag="ACG8H8_001843"
     CDS             8162..9484
                     /locus_tag="ACG8H8_001843"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8986911.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9547..9651
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9725..10468
                     /locus_tag="ACG8H8_001844"
     CDS             9725..10468
                     /locus_tag="ACG8H8_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986912.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10483..11475
                     /locus_tag="ACG8H8_001845"
     CDS             10483..11475
                     /locus_tag="ACG8H8_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8986913.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11487..12224
                     /locus_tag="ACG8H8_001846"
     CDS             11487..12224
                     /locus_tag="ACG8H8_001846"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986914.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12214..12936
                     /locus_tag="ACG8H8_001847"
     CDS             12214..12936
                     /locus_tag="ACG8H8_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP8986915.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13020..>13439)
                     /locus_tag="ACG8H8_001848"
     CDS             complement(13020..>13439)
                     /locus_tag="ACG8H8_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP8986916.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4429 a         2296 c         2791 g         3923 t
ORIGIN      
        1 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
       61 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
      121 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
      181 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
      241 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
      301 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
      361 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
      421 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
      481 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
      541 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
      601 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
      661 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
      721 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
      781 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
      841 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
      901 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
      961 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
     1021 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
     1081 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
     1141 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
     1201 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
     1261 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
     1321 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
     1381 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
     1441 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
     1501 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
     1561 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
     1621 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
     1681 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
     1741 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
     1801 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
     1861 tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
     1921 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
     1981 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
     2041 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
     2101 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
     2161 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
     2221 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
     2281 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
     2341 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
     2401 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
     2461 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
     2521 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
     2581 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
     2641 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
     2701 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
     2761 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
     2821 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
     2881 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
     2941 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
     3001 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
     3061 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
     3121 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
     3181 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
     3241 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
     3301 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
     3361 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
     3421 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
     3481 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
     3541 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
     3601 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
     3661 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
     3721 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
     3781 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
     3841 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
     3901 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
     3961 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
     4021 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtttatt
     4081 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
     4141 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
     4201 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
     4261 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
     4321 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
     4381 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
     4441 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
     4501 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
     4561 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
     4621 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
     4681 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
     4741 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
     4801 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
     4861 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
     4921 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
     4981 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
     5041 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
     5101 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
     5161 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
     5221 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
     5281 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
     5341 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
     5401 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
     5461 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
     5521 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
     5581 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
     5641 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
     5701 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
     5761 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
     5821 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
     5881 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     5941 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     6001 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     6061 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     6121 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     6181 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     6241 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     6301 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     6361 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     6421 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     6481 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     6541 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     6601 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     6661 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     6721 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     6781 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     6841 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     6901 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     6961 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     7021 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     7081 attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag tcaatgtcga
     7141 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     7201 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     7261 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     7321 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     7381 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     7441 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     7501 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     7561 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     7621 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     7681 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     7741 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     7801 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     7861 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     7921 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     7981 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     8041 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     8101 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     8161 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     8221 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     8281 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     8341 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     8401 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     8461 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     8521 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     8581 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     8641 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     8701 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     8761 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     8821 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     8881 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     8941 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     9001 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     9061 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     9121 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     9181 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     9241 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     9301 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     9361 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     9421 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     9481 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     9541 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     9601 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     9661 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     9721 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     9781 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     9841 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     9901 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     9961 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
    10021 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
    10081 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
    10141 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
    10201 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
    10261 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
    10321 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
    10381 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
    10441 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
    10501 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
    10561 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
    10621 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
    10681 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
    10741 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
    10801 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
    10861 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
    10921 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
    10981 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
    11041 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
    11101 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
    11161 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
    11221 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
    11281 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
    11341 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
    11401 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
    11461 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
    11521 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
    11581 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
    11641 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
    11701 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
    11761 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
    11821 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
    11881 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
    11941 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
    12001 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
    12061 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
    12121 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
    12181 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
    12241 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
    12301 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
    12361 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
    12421 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
    12481 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
    12541 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
    12601 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
    12661 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
    12721 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
    12781 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
    12841 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
    12901 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
    12961 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
    13021 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
    13081 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
    13141 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
    13201 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
    13261 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
    13321 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
    13381 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAZKAO010000077        13147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000077 DAZKAO010000000
VERSION     DAZKAO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.077"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..150)
                     /locus_tag="ACG8H8_001849"
     tRNA            complement(78..150)
                     /locus_tag="ACG8H8_001849"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACG8H8_001850"
     CDS             531..1100
                     /locus_tag="ACG8H8_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP8986917.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACG8H8_001851"
     CDS             1087..1947
                     /locus_tag="ACG8H8_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8986918.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACG8H8_001852"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACG8H8_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP8986919.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACG8H8_001853"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACG8H8_001853"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP8986920.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACG8H8_001854"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACG8H8_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP8986921.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACG8H8_001855"
     CDS             5995..6864
                     /locus_tag="ACG8H8_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP8986922.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACG8H8_001856"
     CDS             6919..7728
                     /locus_tag="ACG8H8_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8986923.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="ACG8H8_001857"
     CDS             complement(7831..8460)
                     /locus_tag="ACG8H8_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8986924.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="ACG8H8_001858"
     CDS             8630..9922
                     /locus_tag="ACG8H8_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8986925.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="ACG8H8_001859"
     CDS             complement(9998..10660)
                     /locus_tag="ACG8H8_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP8986926.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /gene="groES"
                     /locus_tag="ACG8H8_001860"
     CDS             10885..11169
                     /gene="groES"
                     /locus_tag="ACG8H8_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP8986927.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="ACG8H8_001861"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="ACG8H8_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP8986928.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccattc
//
LOCUS       DAZKAO010000078        13043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000078 DAZKAO010000000
VERSION     DAZKAO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.078"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..1433)
                     /locus_tag="ACG8H8_001862"
     CDS             complement(18..1433)
                     /locus_tag="ACG8H8_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP8986929.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(1426..2259)
                     /gene="terS"
                     /locus_tag="ACG8H8_001863"
     CDS             complement(1426..2259)
                     /gene="terS"
                     /locus_tag="ACG8H8_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP8986930.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(2418..2648)
                     /locus_tag="ACG8H8_001864"
     CDS             complement(2418..2648)
                     /locus_tag="ACG8H8_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986931.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(3395..3862)
                     /locus_tag="ACG8H8_001865"
     CDS             complement(3395..3862)
                     /locus_tag="ACG8H8_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFP8986932.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(3937..4476)
                     /locus_tag="ACG8H8_001866"
     CDS             complement(3937..4476)
                     /locus_tag="ACG8H8_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP8986933.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            complement(4407..4700)
                     /locus_tag="ACG8H8_001867"
     CDS             complement(4407..4700)
                     /locus_tag="ACG8H8_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986934.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(4731..4925)
                     /locus_tag="ACG8H8_001868"
     CDS             complement(4731..4925)
                     /locus_tag="ACG8H8_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986935.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(5031..5483)
                     /locus_tag="ACG8H8_001869"
     CDS             complement(5031..5483)
                     /locus_tag="ACG8H8_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986936.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(5509..5970)
                     /locus_tag="ACG8H8_001870"
     CDS             complement(5509..5970)
                     /locus_tag="ACG8H8_001870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8986937.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(6171..7022)
                     /locus_tag="ACG8H8_001871"
     CDS             complement(6171..7022)
                     /locus_tag="ACG8H8_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8986938.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(7037..7837)
                     /locus_tag="ACG8H8_001872"
     CDS             complement(7037..7837)
                     /locus_tag="ACG8H8_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP8986939.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(7880..8770)
                     /locus_tag="ACG8H8_001873"
     CDS             complement(7880..8770)
                     /locus_tag="ACG8H8_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP8986940.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(8772..9713)
                     /locus_tag="ACG8H8_001874"
     CDS             complement(8772..9713)
                     /locus_tag="ACG8H8_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFP8986941.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(9946..10281)
                     /locus_tag="ACG8H8_001875"
     CDS             complement(9946..10281)
                     /locus_tag="ACG8H8_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986942.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(10340..10468)
                     /locus_tag="ACG8H8_001876"
     CDS             complement(10340..10468)
                     /locus_tag="ACG8H8_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986943.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            complement(10437..10610)
                     /locus_tag="ACG8H8_001877"
     CDS             complement(10437..10610)
                     /locus_tag="ACG8H8_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986944.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            complement(10607..10795)
                     /locus_tag="ACG8H8_001878"
     CDS             complement(10607..10795)
                     /locus_tag="ACG8H8_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986945.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10873..11097
                     /locus_tag="ACG8H8_001879"
     CDS             10873..11097
                     /locus_tag="ACG8H8_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP8986946.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(11094..11249)
                     /locus_tag="ACG8H8_001880"
     CDS             complement(11094..11249)
                     /locus_tag="ACG8H8_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986947.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(11389..11601)
                     /locus_tag="ACG8H8_001881"
     CDS             complement(11389..11601)
                     /locus_tag="ACG8H8_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986948.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(11604..11816)
                     /locus_tag="ACG8H8_001882"
     CDS             complement(11604..11816)
                     /locus_tag="ACG8H8_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFP8986949.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            12091..12501
                     /locus_tag="ACG8H8_001883"
     CDS             12091..12501
                     /locus_tag="ACG8H8_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986950.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            12514..12966
                     /locus_tag="ACG8H8_001884"
     CDS             12514..12966
                     /locus_tag="ACG8H8_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP8986951.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
BASE COUNT         3673 a         2732 c         2031 g         4607 t
ORIGIN      
        1 aatcaaaagc tcacctccta aaattgaata aattgatagt gacttggtgg ctcataaaac
       61 gctaaagcca gtgcgtcagc aatatcggga ctaccaatat ttcgcttttt catatcatct
      121 ttactttcta aacgtatacg acttctactt gtcattttga atttgcgagt acttaattct
      181 ttgattaacg aactatcaga aggcaattca ataacaggct gttctccgtt aagatttgct
      241 gtcatatttt cttctaacat ttccttgatg tttccccata gttgagtacc taaattatcg
      301 taaaaatcgt cttctgatgt tgaaccgtta ttcactccaa acacctcaaa aggataatgt
      361 ttgtcttcta taagttcttc taggcggtcg gttacaccac ctccgacacc agtgtcatcg
      421 actttaatca tcactttatc aatactcgga tattgactca ttagattctt ggccatgttg
      481 atgacatatc ctgttgtttc catggtgcta cgttttgaat acttctcata ctccaatgcc
      541 ctggtagcaa ttctaggaaa gagaatcgta gaatcatcac catatcgagc tacgtcgaca
      601 ccaatatgag caacctttgt tttattgact aaagaatcac taatttgttt ttctgtagcc
      661 aattcaactg tttcaagact gataaatgaa tccaacgcac ctttcggaaa ttctccaaaa
      721 atacggacac gagcaacatc actttctttt ccatattttt taagaatcat ttctatattg
      781 tctttgtttg tacgtttact atcatagctt gatactttat gaactctgta tttatcacgg
      841 tctgaattat gggaatcata aaagacacct tcaatattat tggggtttcc acacatcaac
      901 agcttattat caaaacctga tagcgtacca agaatagctt ccataattgg atcagacaca
      961 ccagaagctt catctaccac aatcaacata tggtcctcgt gaaaaccttg catactttct
     1021 ggtttagtcg ctgttctagc cgtagcaaac caacgctctg aatcaccaac catataaatt
     1081 ttggtctttg tccacttcag taagtttttg atcaagctat catttaacca tttagctacc
     1141 tctgcccaaa gtacatcgta aagttgcttc attgtcggag ctgttgctat tactttagcg
     1201 tagggtcgac acgttaagaa ccaaagtatt gctcctgctt ctaatgctgt ttttccaact
     1261 ccttgaccag aacgaacaga aacctttgaa aattcagcta aatcatttaa aacattttct
     1321 tgccattcat caggattcaa atgcaaaata tcttggcaaa aagctactgg tttatcatag
     1381 taataatcaa tggcagaacc aatatcagca aaaggaataa attcattatt cattttcttt
     1441 caccgcccgt ttgtttgcgg catttaaaac tgcctgtttc cattcttcta tttcttcacc
     1501 tgaattattt ccgccaattt catttgtctc agcttggatc ttatcagtct gagctttaat
     1561 taaatcagtt ctgtattcat tcatataaag ttcattcatt tgcttgatgg ctttagctaa
     1621 ctggttgcta atccgagtca atgaatcttc aattgaaaga atatcatcaa ttttgcgatg
     1681 tttttttctg ctaatctgca catcttgcat aacctcacgc ttgatttcta gctttttacc
     1741 atttttttca atcggagttt taatctttct tagctgctgc aatcgttcga cttcttcatc
     1801 atttagtcca gcttcagctt gttggattct tttcatcatt cgaagttgtc ttattttaag
     1861 aagtcgtatt tcttcggaca aagcaaaaga aggatcatca ttcaaactag aatagatgtc
     1921 cttctcatta tcggttaacg tgtcaaaaaa tattgtttca tactcacctg ttttcaaagc
     1981 gtttttatta cccactggcg gagaagctcg gctatttcct ttgttacctt tagcattttg
     2041 attaccaata ggagcgccac cttgattagt aacgttactt ttgccattgg taacgttact
     2101 aatcaattca gcgctccatt tgtcttgcga tttccatttt ctaacctgag aatctgaaac
     2161 atttaattca gatgcaattt cctttaactg cttttctccg ttggattcta accaaattct
     2221 tttggcttca tcacgtcttg gatcacgttg tcttgccatt caatacacac cacctcacat
     2281 tctgtttagg ttgagttttg ttttctaatt ttcaatatct tttagcatta aatcagcttc
     2341 aatcaatatc tttaaatcgg aaactttgtc taacttgatt tgtcctgatt ggagattttt
     2401 aagccatttc ttgcattcta atccctcaaa ttcttgttaa tattattttg aatgtttggt
     2461 tcatcataga aaccatgacc gcaataaacg agcttgcacg catcaatctc acatggttct
     2521 gcttctctcc ccatttcgac aatagagtac ttccctttga tttgtacaga acgcacaaca
     2581 cgcactgaac aatcatcaat ggttcgagga tattcattag ttagcgatac ataccagtag
     2641 tttctcatta tgtagcctcc tttatgtaaa gaaaaagacc actcaaagag tgatctaata
     2701 tgtaatgcac aggcagggac gtttccgatc ctgtgcttga gacatttgac gattcatttg
     2761 taccgaatcc caaatctcaa tctaacctaa ccagttatgt aatagcaacc tacacgatgc
     2821 acaaaacgcg tacgaaattg cgcaccccta tatttttaaa ccgccgatgc ctcggttgcc
     2881 aaagtcactg gcaaggaatc gaaccttgca tggttgccga agcattgacc tagcacacat
     2941 gcttagcgtc taccctttcc gccacagtga cactataaaa ttattcttgg ctgctactat
     3001 tttttatttt gcccattttt aaatccaatc atatatacat taagacaaag cgcaaaaatt
     3061 gaaattatta atggaatcat ttctcttcac ttacctttag ttatcgtgtg aataattaaa
     3121 aaaacaatag acagcaacgg atgatagata ataagaacaa tttagaagga gttgaaattc
     3181 acatccttat tcttaatatt tccgttgctg cctatcgaag cttaattaaa cgatgaggga
     3241 gatttcctcc cttacatttt attttgtcga tcctgtttcc taatttttcg acactatcat
     3301 aatacaacgt tgaataggta agtgattggt ataaaaaagg tataaaatgg aaaccaaatt
     3361 ggtaataaaa gggtataaaa agtgtaaaaa ctggctactt gaaagcaacc agttctaacg
     3421 atgaagcaaa ttggatgata attctgtttg attctacttt aaccgattct tcgctagtat
     3481 tattcctttg agcagttaca taaatgggca gaccattgat ataacgatca tagaatatct
     3541 tcttgcgcct ttcagtcaca tcaggcttat gcggatgctg tatcgctgaa tagcctcgaa
     3601 caaacaattt atgcagatac tcaaattctt cctgtgcttc ttctttatcg atcagcattc
     3661 tttctgcttc aaatatatga tcagctgtag aaggtggaac caaggaataa gatgctgtca
     3721 cttttggttc tcgatgttgc cctactctac atctagctga tagatatgct gaaagaaaca
     3781 cagcgacatt atgttttgtg cgttccatat caacatcttt cgcgcttggt gtctcatatt
     3841 tctttacatc gaaaagtacc atcctctgat tcccccaatt atgatataat acttatgtcg
     3901 gaaatattat tcatagtcgg aggaatccga ctttttttat tttgtacgtg aaatgagttc
     3961 tcctgattta tacgcctcag caaattcaac caaagctact gccttcattc tctcgatagt
     4021 tctatcggaa tatcctaatt tttctcctat ttgatacatt gacatcttat taggaaaaca
     4081 ataactataa taaagtacct ggaaatggat tctacttagc cgtgttaatg cttcgataac
     4141 cgaatctcga gtacttatcg cttcaatgat ttcttcgttt tgactgtcaa ttgtgaattt
     4201 caaatttttg gtaacagtca tcacggaaaa gtctattttc actggtccga ccaaacgttc
     4261 taaatttctg tatcttttta gtaatcgtct agcatttttc cttgtttgct taatatctat
     4321 attctctaaa atgttcacaa ttgcctcttc tccttccagt tgtggaataa cgctcattgt
     4381 tgtaggatag ttgtaaaaaa gcaaaattaa gattttataa cttccatgtc caccaatctc
     4441 accactgcta aattctcttt gcttttcgct aaccacttat cacattccat cgtgttttca
     4501 atgcgaatga ttgctgagtg attatagacg tgttctacat atccacgaaa tggatagatg
     4561 aatccttctg cttcgcagcg aaccatgtca ccgactttga attttggttt cttacgtgtt
     4621 ttaggattct ttgtcggcat atctagcatt aaaccgccga tgccgtggcc gctggagtaa
     4681 aatccgtctt ttagtttcat tccgcttcct cctctttagc tgtcaattac ttactcgcaa
     4741 gtcactaaaa tggctacttc atcgataaag tttccgtctt cgtcttccat tccttgctga
     4801 atgctaatgt cggtaatctg cttaaaaaat tcgttgtcat agccttgtgc gtttatcact
     4861 acttcgacgt ctccgaaaac ctctttgaga ctattaattt tatttactac ttccgatgcg
     4921 tccatgacta cacccctttc cagttaaaaa tctctgtcgt taatagacta ctgttcaaag
     4981 tattcatctt cactcatcct tctgcctcct ctttagttgt cattagtgga ctaaatacta
     5041 cctgtttgaa aaccataatg tgcagtttca acgccgtcat aatcacttat ttcccactca
     5101 gccccatcgg gtatatcaac gactttaaga cttgcatatg gtccactggc ctcacttcct
     5161 agtttttcga caacagcaac gaggtcctta tcacctctgt ttgaatcact tgaaaaactg
     5221 gaatcatcag caattccgta cagtcggtca atttcgctat aaattcctgt aactttgtcg
     5281 attttcgggt cttttttgaa atagtagata aaatccatcg aacctagctt ttggcctgtg
     5341 tatctcgtgt attcatcatc tgagtcatag ctaaagttat tgatgtatgg gaatacttca
     5401 atattttttc ttttaaaaat ttccattttt gcttcatggc tcaacccaaa gccaccaaaa
     5461 catttattta atacaatctt cattactttt cctcctttgt ttcctccgtt agctgaattt
     5521 tttcataaac acaagccaat gtgttttgct tcgtttatta ccaaacaatg gctgttctcc
     5581 aatagcctct aacacatcag aaagtggtat ctgttcctcg ttccacttaa aaattaatgt
     5641 tcctgaaggt cgtaaaactc gcatacactc tgcaaaacct tgcctgatgt cttctggcca
     5701 aagttcgtct aattttccat attttttggc aagccatgag ttttcgccga catgtcttaa
     5761 gtgtggcggg tcaaatacaa ccatatcgaa cacatcatct tcaaaaggca tatcgcgaaa
     5821 gtctgcgacc acatctggat caacattgat cacatgacct gttgataatt cttcatactg
     5881 ctgacgacaa tccataaatg ttacgtcaga attgttctta tcaaaccaga acaatctact
     5941 accgcaacat gcatctaata tttttggcaa ttactctacc tccaataact ctggattctc
     6001 gtagacgtta cctaaaacag tgagaaatct ttctgtatcc cgataaataa ttagcttact
     6061 tatcttccga accattcgac tctcttgttg agataattcg tccatgtcac gatcacctat
     6121 aatttgcaac attatctgac gacagattct tttttcttgt ttagtcaaat tcatgcgatc
     6181 gctcctttga ctggttttat gcgcttgtct gctgtttgag aaaataccat cgtaaatcca
     6241 tcagagttga caaacattct tgatattgtt ctcgtaccgt aagctttttt cagttcttca
     6301 ccaagcaagt tcgttgtgat aatcgttgct aggttctgtc gtgcatccaa gaacgaattt
     6361 agcgtgttta tgccaaacgc tcgactatct gatgcatctt ttcctagttc tgaaccgata
     6421 tcatcaatga tgactaaatc cgctgttttg atatctgcaa tcaacgatcc ctcaatcgtt
     6481 tttctgagtt caggattgtt gtacgaaaat ttgatttgat ccagcatttc ctgtaacccg
     6541 ataaataaga tttttttatc gtagtttgat cgcttcaaga cttcccaagc cgctgccatc
     6601 gctaggtgac ttttacctgt tccttgtttt ccagttaaaa caaggtgact tggatttcct
     6661 aacaagactg aattaacaaa gcgtttggtt acttcaaccg cttgtcttgt ttcttgatcg
     6721 acaatttgat aattctgcaa tgtgcaatca aacaacgctt gattaggcac aacagaacca
     6781 cctttgaaaa aattgattgc tcttgctttc aggctctcgt tgtaaattcg ttctgtttgt
     6841 aagtcttcct gaactcgcaa tgctttatac ccacattgca tacaggttgg cttacaacgt
     6901 tctgagccat cagggttctt cgtacgccat ccatacaaag gctgtccgca ttcaggacat
     6961 tcgccacgtt ggacaagcac tttctgtatc agcttttcca tgatttcccc aacagtttcc
     7021 atgcctacgc ctcctctcaa ataggcaact catctgtgct aaatttctcg tattcaagcg
     7081 ttttagtttg ttttgtttta taatcacgat ttgcttgcgc tgccattgtg tcgtattttt
     7141 ctcgcaattt tttagcagat aaaatattcg atgcccaaaa cacattgtgc tgactccatt
     7201 caatcattcc ccgtacctgg ttttcggtcc gcttgtcgat ctcgatcatt ttccgaatgt
     7261 catctgccca actttgcaga ttcggctttt tgatttcctg attctgacaa atctgtttga
     7321 ataactcctc cgcgagaatg tagtaaactg agtcggtgtc gtaaacacgc tttttgcgtg
     7381 gttgcgacga tgatgtttta ttattctttt cattcttata attcttttca ttcttgtttg
     7441 tgtgcacttg ttgttcactt gttgtgcgtt tgatgttcat ttgttgttca ctgctttggt
     7501 acaaagacca gttttttatt gatataacgc tgtatttcgt agttgatttg atgttcaaca
     7561 ttccgttttt ttcaaattgt tttagccatc tccatacaga accgctgttc acttgatgtt
     7621 cacgtttcac gccttcgttc atctcagatg tgatagcatc gcgccctgtg acgaattctc
     7681 cgctgttcac ttgtatctct tttccattaa aaagtatctt gcgattctca tgactcgctt
     7741 tcatcaaaca caagttccat aatttataca tataaggatt ggtccaaacg aatgaattca
     7801 tcacttttcg atgtaatttg acatatcctg tgttcattcg tatttctcct ctagaaaagc
     7861 aagggagaaa gctccctcat tatttgttta acggcggatt agatgcatca aataatccag
     7921 tttgtacatc ttcattttct tcagatataa cctctgcttc ttttctttca ggaatatctt
     7981 cttcaacttc tgtttcagca ataatgctgc cgtcttcttg aaccctttgg actctctcat
     8041 ccgatgtggt ggcttcttgc atttcgatgg acaagattcc ccatttagaa agaagatttc
     8101 tcaaaacagt ttttcgtgcc attgcattgt aatcagatgc ccacacacca cttaactttg
     8161 tcttatcgcg atctttattg ttagcaatcc gatgagcttc gatttcttgt ttggtccaat
     8221 agacagtttt tttgaatcca ttcagtaact cgaaatagcc aacatatcca atgacttcat
     8281 cagacgttct accatttgga tcaaactcaa actcttctgt cagtcggttc cagcttttta
     8341 gttctccttc gtaaacttcg atcacattta atgctttgta tttacctgat cgttgggcta
     8401 attggatata tcctttatag ccaagcatga attgagcttt cttttcccat tttcctgttt
     8461 gcttgttttt actattgaat ggaactaaat atgcataacc taaattctta tctagcccaa
     8521 gatttaatgt tgcagcagtt aacgcaccac tcatgataga catcggttca ctatctgcaa
     8581 gataactgtc attagataca agagtcataa cattcgacat aaaagcatta gcattgtcat
     8641 gaagcacctc ttcgaacttc tttctcattg ttggtgtatt catcaaagct ttaagtccta
     8701 attgacctgg tgcaacttgt ttctgtggct ttgctgccaa ttgatttttt aacgattcat
     8761 ttgttgccat attatttgat ctccctttcg gttagccttc ttgattcagt aacgttataa
     8821 atctcttcat catttgcgac atctggatat ttctctgcta gtttcttcga gttcatacgt
     8881 ctcgtacgga caaatttcca actgatgatg tttttttgag tgataccgat actggcttca
     8941 cgtttaccta gctcgctgat aatctcgttg tctacttgac ggatagctga ttcaatttct
     9001 ttcttagtcc gtttgagttc gtttttctgt tcaactagtt catcgaaatg aattggtaac
     9061 gtcgtttgaa cgtcttcaac atctgcatac ttctctttca agaaatcagc tgtcgcttga
     9121 ctaccatcaa taattggctc gatacctcca agaacgtttg tttcccaaaa ctctactaat
     9181 tgttcagtga ttgtatcgat cagctcttga tctcgttcaa tccgcttcca gataaatttt
     9241 tgaccaccga ttaagacagc gatgtaacaa tagtctttgt ttagaacgtt catgtaatgt
     9301 tgaacttgac agagatagct aagcggtacc tcttcgcctt cccattcttt accgagaaat
     9361 tgattggctg ttttgcattc cagaatggca ttttctccca ctacttcccg atcaatattt
     9421 gctcttaaaa atggatgtaa tggatgttca aatacttggt ttcttctgcg tacttttttg
     9481 cctgtacgtt cctgaaactc tttagcaacc acttcttcta aaacattacc ccaataagcg
     9541 ggctcacttc ctgattcttc aagtactact tgtcctgttt tttctagcca tagttgataa
     9601 ggagatttcc atttattcaa tcctaaaatt gttccaacat ccgaacctcc gatgcctttc
     9661 ttacggtctt caagccattc ttgatggctc atttctaagg tagatttact catcttcatc
     9721 ctcctcatca attggtgctt catatggtgg tttagcataa tcaggatcag ttaaatagtt
     9781 gtcaagattt gctaactcgt tcatgtttaa cctcttttct gcttgctaac acgcaaagca
     9841 caagcatggt aattgcgaat aacaagccaa agattacata acttttcgct acaatcacta
     9901 acgtgaaaaa taaaataatg agtagatcaa ttgttttttc attcatcatt gaaattcccc
     9961 cttgagaata cgagtcatca catcagctaa atcttccttg ttattaataa caaaagtatg
    10021 atttgaaggt tgattttctt cttcattttt cgcttgttcg taagcatcca cagcaattct
    10081 acgcataata tgtgctgaca atgggagatt ttcatctaaa tgttcttctt gcatagcaag
    10141 acaaagtaaa atttctgttg tctcaccata aacgagactt ttaagttccc cttctttttg
    10201 ggctgtacaa ataatagaaa caccttgctt ttggcattca cgtttcaatt cttcgataag
    10261 attttcaatt ttcttattca tgtgatataa ttctcctagt tttataaatt tgtatgtgac
    10321 tctttgctgg ctggcagagt cactttttta tttgttgcca tgctttttgc ttttcgatat
    10381 gttgcttgct taaaataata gggcggctat ttccccaccg attatcagca atcactttac
    10441 cgatttttag cgcttcttct cgtgccatag ttgctccttt cttttgaatc aagcagattg
    10501 attaaaacca tcaatgctgc gaacaagctt cccccgataa tactttggtg cgctacaatc
    10561 actaataacc ccaaaatgaa tcctataaaa agtgtgtctg tcttcttcat aatctaatct
    10621 ccctattttt tatttctagc attctcaaat cctcaagttc agaagcaatt agttcagctt
    10681 gcctatctga tagctcatcg gcttttctaa gcgctgcacg gtcatcttgt aattgtttcc
    10741 tgcgttgttt aatcaaaccg agaatttgat gttcttgttg caaggtatat gccatatcac
    10801 ttctcctttc gtttattgaa tttctccatt tctatttgat aaaatatttc tatagaaatg
    10861 gaggtgaaaa ctatgggaaa aaatcaacat gttgtaccta attccaatgg tggttggaat
    10921 gtcaaaggtg ctggaaattc taaagcgact gcacatacta atacaaaatc agaagcagta
    10981 aaaatcgcta gagagatttc taaaaatcaa ggatctgagc tatttattca tggcagagat
    11041 ggaaaaattc aaagccgtga tagtcacgga aacgatccac acccaccaaa aggttaatca
    11101 tatttaggag ttaatcgaat acgataaccg tcagcaggag ttgcatcggt caatgtaact
    11161 tctgctactt ttttcccttc ttctgtttca ataattaatc ttgtgtaatt gttctcattc
    11221 aaaatcccta tttttgattc ttccttcatg tcatccctcc ttcgtctctc gccgtcacca
    11281 cctccaagaa gaggagttgc caaaatgaaa aaacgtggac tttggcaaac ttgaaaggag
    11341 agcagcaact cctcttttca gaagtggtga atttagattt tttagctatt aacaaacagc
    11401 tctaactcta aatcgatttt tagaacgcca tgttctaaac tgttcatata aatcgacatc
    11461 aattgttatt tctttggaag taactgcgtt atacccctca ctaaaacgtg attttttaaa
    11521 ttccttcatt cttctttgat aagtactttc agaatactta tatttctctt taaactccgc
    11581 tttgctcaaa cgttgtacca tttttacgcc tcctgttctg atggctgatc attagaatct
    11641 acttctaact tgatatccaa aagttcttcg atttgtttta aacgttcctt aggttttctt
    11701 ttttcgatga agatatctcg taaataagta attgatatac ccattcgtcg agctaactct
    11761 ctttgactcc aacctttctt tactaaagca attaatactt gctgttttaa agtcatgctt
    11821 cataccccct tatcgaatat tttctaatat ttttgtctaa taagtttgac aaaaactgca
    11881 aatatgctat tatgtaagca tacaaaatac agcgcacaaa aagcgagctt actgatgtct
    11941 ggggagacta ttagtttatt gcttttagtc tttgtatgcc gtaaatatct tgcttacaaa
    12001 actagtataa ctgcaaattc tctatttgta aatagataat ttgcagaaaa atactttttt
    12061 tgtcggtatt ttgaaaggaa tgcatatcct atgctattag accgtataaa aatcttatta
    12121 aaagaacgtt caatgacgtt ggcagaactt gaaagaaagt taaatttcgg aaatggaagc
    12181 atgtctagat ggaataaaag tactccatct ggagataaaa tacaaaaagt tgcagattac
    12241 tttaatgtat caactgatta tcttttagga cgtactgata aacgccatta ttatgattta
    12301 acagaaaaag ataaacaaga tattgcttta caagctgaaa aattacttga aggcttgaat
    12361 tcagatgctg aaacaaatta ttatggtgaa cctttagacg atgaagataa ggaaaaatta
    12421 tacgatgcca ttcaacttgc acttgcctta accaaagtta aagcaaagaa aaaattcact
    12481 cctaaaaaat atcgtcaata ggtgggcttt ggtatgcatt taaacaaata ctggatatta
    12541 gaagaaatag aaaaattatt tgtagagttt ggatctgatg cgattaatcc atataaaata
    12601 atcaaatatt ctgatataga tttaattgaa actgatttag ggaataaaac attaggacaa
    12661 acgatacaca ataaaagatg ttatgtgata ttaattaatc aaaacctatc accttctatg
    12721 aaaagatttg tattatggca tgaaatagcc cacgttagac tccataaagg tatttgcaca
    12781 gcagctttta gagcaaacaa ccttagttgt atgatttatg gtatagaagc tgaagctaat
    12841 atgtttgcaa ttgaaatgtt aactagaaca atagatgaac aagatttatc tgatatgagt
    12901 cagttccaaa ttattgaata tttagggctt cctcataatc ttgaacattt agtattacaa
    12961 cattaaaaat aaaaaagccc atgctttcac atggactctt tcctcatttc tgagtaaact
    13021 ctacaataat tatatcaaag aaa
//
LOCUS       DAZKAO010000079        12734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000079 DAZKAO010000000
VERSION     DAZKAO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.079"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8H8_001885"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8H8_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP8986952.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG8H8_001886"
     CDS             complement(322..891)
                     /locus_tag="ACG8H8_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP8986953.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG8H8_001887"
     CDS             1077..1979
                     /locus_tag="ACG8H8_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8986954.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8H8_001888"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8H8_001888"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP8986955.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8H8_001889"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8H8_001889"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP8986956.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8H8_001890"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8H8_001890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP8986957.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG8H8_001891"
     CDS             8547..8732
                     /locus_tag="ACG8H8_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP8986958.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG8H8_001892"
     CDS             complement(8794..9990)
                     /locus_tag="ACG8H8_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP8986959.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG8H8_001893"
     CDS             complement(10160..11596)
                     /locus_tag="ACG8H8_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP8986960.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACG8H8_001894"
     CDS             11889..12698
                     /locus_tag="ACG8H8_001894"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8986961.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2673 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZKAO010000080        12299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000080 DAZKAO010000000
VERSION     DAZKAO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.080"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACG8H8_001895"
     CDS             complement(<1..274)
                     /locus_tag="ACG8H8_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8986962.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQ"
     gene            complement(271..924)
                     /locus_tag="ACG8H8_001896"
     CDS             complement(271..924)
                     /locus_tag="ACG8H8_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8986963.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1212..1732
                     /locus_tag="ACG8H8_001897"
                     /pseudo
     CDS             1212..1732
                     /locus_tag="ACG8H8_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1730..2449)
                     /locus_tag="ACG8H8_001898"
                     /pseudo
     CDS             complement(1730..2449)
                     /locus_tag="ACG8H8_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2400..2609)
                     /locus_tag="ACG8H8_001899"
     CDS             complement(2400..2609)
                     /locus_tag="ACG8H8_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986964.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2591..2659)
                     /locus_tag="ACG8H8_001900"
                     /pseudo
     CDS             complement(2591..2659)
                     /locus_tag="ACG8H8_001900"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            2886..4159
                     /locus_tag="ACG8H8_001901"
                     /pseudo
     CDS             2886..4159
                     /locus_tag="ACG8H8_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4266..5297)
                     /locus_tag="ACG8H8_001902"
     CDS             complement(4266..5297)
                     /locus_tag="ACG8H8_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8986965.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5304..6134)
                     /locus_tag="ACG8H8_001903"
     CDS             complement(5304..6134)
                     /locus_tag="ACG8H8_001903"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986966.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6131..6946)
                     /locus_tag="ACG8H8_001904"
     CDS             complement(6131..6946)
                     /locus_tag="ACG8H8_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8986967.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6943..7767)
                     /locus_tag="ACG8H8_001905"
     CDS             complement(6943..7767)
                     /locus_tag="ACG8H8_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFP8986968.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7757..8857)
                     /locus_tag="ACG8H8_001906"
     CDS             complement(7757..8857)
                     /locus_tag="ACG8H8_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8986969.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9035..9820
                     /locus_tag="ACG8H8_001907"
     CDS             9035..9820
                     /locus_tag="ACG8H8_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP8986970.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9853..10236
                     /locus_tag="ACG8H8_001908"
     CDS             9853..10236
                     /locus_tag="ACG8H8_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP8986971.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10312..10443
                     /locus_tag="ACG8H8_001909"
     CDS             10312..10443
                     /locus_tag="ACG8H8_001909"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8986972.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10412..10648
                     /locus_tag="ACG8H8_001910"
     CDS             10412..10648
                     /locus_tag="ACG8H8_001910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8986973.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10660..10746
                     /locus_tag="ACG8H8_001911"
                     /pseudo
     CDS             10660..10746
                     /locus_tag="ACG8H8_001911"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10726..11697)
                     /locus_tag="ACG8H8_001912"
     CDS             complement(10726..11697)
                     /locus_tag="ACG8H8_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP8986974.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3690 a         2773 c         2234 g         3602 t
ORIGIN      
        1 tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca ctactcttgg
       61 aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata ttactagaaa
      121 tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt ttaggaaaga
      181 taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa aatatttgta
      241 tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat atcctacatt
      301 tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta tatatacatc
      361 tattactcga tcataaggag tttcttcgct atctttccat acataatcaa taatttgcat
      421 tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta gttcttttgc
      481 atttacatct atttcttcac catttatttt agctgtatag ctattaaaat taaccgatag
      541 atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag aatctattct
      601 agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta aattagtaaa
      661 tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg gtaaattgct
      721 tttctttcta atttctttca acacttctaa accatttata aaaggtatct gaatgtctaa
      781 gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat ctttagcttg
      841 aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta tcatattatc
      901 atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac tagatattat
      961 acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
     1021 ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa aggcttctat
     1081 aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc
     1141 aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa
     1201 ggagtcgctc tatggataat catactagaa aattactcgg attaacagat aaacatttat
     1261 tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta
     1321 aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc
     1381 gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag ttgactattt
     1441 tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa
     1501 cggacttagt tgatgaacat catcaactaa caaaggaact gaaacaggcg attctcatag
     1561 aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg
     1621 atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac ggcattatca
     1681 tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat
     1741 ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca tcacggggat
     1801 ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt
     1861 aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc agttcttgct
     1921 cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct cctgcaatgt
     1981 tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg taaagattga
     2041 caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac aattgacgtt
     2101 tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa
     2161 aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg gcaccgactg
     2221 gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac aacaaataac
     2281 cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa tcaatttttt
     2341 ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca gaattgagac
     2401 tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc cactggtctc
     2461 ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc aacaccttgt
     2521 tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc ttcagtaaat
     2581 agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca tcaaaatcaa
     2641 aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct
     2701 gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg
     2761 tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt gagcataaaa
     2821 aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc aaaggagcgt
     2881 tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat ggaaacattc
     2941 aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca
     3001 cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga
     3061 tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga
     3121 cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc tattttacgg
     3181 cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat tataaaatat
     3241 tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc ggcgtttctt
     3301 ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg aaaataaatt
     3361 ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct
     3421 attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt ccttcacgta
     3481 agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa
     3541 aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag tgctttcctc
     3601 atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc agttttaagg
     3661 atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa gccaaacact
     3721 atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg cttgtttatg
     3781 ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt gaagtcgtag
     3841 aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat caagaaattc
     3901 ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa acaatgccca
     3961 agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat gaatccggca
     4021 ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg catacccaca
     4081 tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt tcttaatcaa
     4141 tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa ttctacctta
     4201 gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg tttgccgact
     4261 atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat tgctagaata
     4321 tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt caaccgatcc
     4381 actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt gcctaaaata
     4441 aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc aaattgaatc
     4501 agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc gtgggtatac
     4561 tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc aacaaatcgg
     4621 ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg cccagcttcc
     4681 atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc
     4741 atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg ttttctaatt
     4801 cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc agtgaccaat
     4861 gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac atttttatct
     4921 gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg aatcacctca
     4981 ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt catagttgga
     5041 aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg cttatcggca
     5101 atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc cgtgatacag
     5161 cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag ctcacccttt
     5221 tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag attgagatca
     5281 tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt cgactgcttc
     5341 tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag cataaaggta
     5401 gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct agccaaatgg
     5461 ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg accgccaatc
     5521 aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag agaaactagg
     5581 gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga catcggggcg
     5641 cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat ctggttgtta
     5701 agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa cactacaaac
     5761 attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat cactcctggt
     5821 aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg gaaatacaaa
     5881 tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac cacacctgct
     5941 aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag ctgttggaac
     6001 cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga gctggcatac
     6061 aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa aatcggtttt
     6121 attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac attgccgtta
     6181 ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg gcgaacacat
     6241 ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg ctggtcaggg
     6301 catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag gtgctggcaa
     6361 tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat gaagcgccca
     6421 gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga tacaaaaaca
     6481 agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac caagaataca
     6541 aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc cccaacaaaa
     6601 taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg gaaacaaagg
     6661 tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa ataaacgtcc
     6721 cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca aaggcctgtg
     6781 tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg ttagttaaat
     6841 cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat gcacagaaga
     6901 taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc tcatcaattc
     6961 ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa acaatggagc
     7021 cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg aaatcaaata
     7081 aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg catccatccc
     7141 gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa ggtaaatagc
     7201 caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg cggtaaattt
     7261 gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct gatactgatt
     7321 cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct cactataaaa
     7381 gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca aacgattata
     7441 gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg tttttccgcc
     7501 agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat ggtaattact
     7561 atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata acggacgtga
     7621 attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct caactaaatg
     7681 attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta gtataactac
     7741 aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa agctctggaa
     7801 gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc gcttcataag
     7861 catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg cgaataggtc
     7921 gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat tcccgtgcta
     7981 gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac ccgctaatga
     8041 tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg tcacgataat
     8101 ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca agatttgcta
     8161 cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag taattcagac
     8221 ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat acagcaaatt
     8281 gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc tcttgcagct
     8341 ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag gaaatggttc
     8401 ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga atttcctccc
     8461 aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga ccgaagctga
     8521 taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac ttagccaatt
     8581 cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt tcaaggtcgt
     8641 tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca atctctcctt
     8701 cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg
     8761 ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca caaaataaac
     8821 cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc ctttcgataa
     8881 ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca
     8941 atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct tctaaagctt
     9001 ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc agcatctcca
     9061 cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct atcagccgga
     9121 gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa aagatgacat
     9181 tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct attccaccaa
     9241 atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga tcgtggatta
     9301 cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg gccgctttga
     9361 ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg aatattttga
     9421 tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc actttgatta
     9481 tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg aggctcagct
     9541 gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa atgccaagag
     9601 cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt atcaaagtgt
     9661 cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt atcagctggg
     9721 ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc tagtcattca
     9781 aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata atagaagaaa
     9841 gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat ccattattta
     9901 attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt gttagcagcc
     9961 gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt
    10021 caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc gcagtttcat
    10081 cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca gctgcaattg
    10141 acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg ccttggtgat
    10201 caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg cgtttcagtt
    10261 gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc
    10321 accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct ggctgtagcc
    10381 aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat
    10441 taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag ctgtttccag
    10501 ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta atggcgcctt
    10561 ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg tgcaaaacac
    10621 aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg aggactaccg
    10681 gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga aatcgtagca
    10741 gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt gttcattcga
    10801 caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca aaagctctat
    10861 tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa agcttggatt
    10921 caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc
    10981 cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg
    11041 atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg gtagtaattc
    11101 taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca gctcggtcag
    11161 gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt taggatactc
    11221 aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg tccattgttt
    11281 gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat agggagctac
    11341 cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat tgattccaat
    11401 gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc
    11461 gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc
    11521 cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct tgctttgtga
    11581 ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa caacggtccg
    11641 taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc cttccatata
    11701 gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt accatttaat
    11761 cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa aatctggtca
    11821 aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct aaatcatttt
    11881 ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta gatatcaaac
    11941 ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt gaataacatt
    12001 caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa aaaatatata
    12061 cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt accttaaatg
    12121 attccattta ctttttatct cattttttcc tactagttag tatcaaatac tctcctattt
    12181 tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt gtcattacag
    12241 caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat gtctacagt
//
LOCUS       DAZKAO010000081        12185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000081 DAZKAO010000000
VERSION     DAZKAO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.081"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..632)
                     /gene="frr"
                     /locus_tag="ACG8H8_001913"
     CDS             complement(75..632)
                     /gene="frr"
                     /locus_tag="ACG8H8_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP8986975.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(635..1357)
                     /gene="pyrH"
                     /locus_tag="ACG8H8_001914"
     CDS             complement(635..1357)
                     /gene="pyrH"
                     /locus_tag="ACG8H8_001914"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP8986976.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1493..2374)
                     /gene="tsf"
                     /locus_tag="ACG8H8_001915"
     CDS             complement(1493..2374)
                     /gene="tsf"
                     /locus_tag="ACG8H8_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP8986977.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2473..3255)
                     /gene="rpsB"
                     /locus_tag="ACG8H8_001916"
     CDS             complement(2473..3255)
                     /gene="rpsB"
                     /locus_tag="ACG8H8_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP8986978.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3614..4093
                     /locus_tag="ACG8H8_001917"
     CDS             3614..4093
                     /locus_tag="ACG8H8_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8986979.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4310..5401
                     /gene="phnW"
                     /locus_tag="ACG8H8_001918"
     CDS             4310..5401
                     /gene="phnW"
                     /locus_tag="ACG8H8_001918"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP8986980.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5394..6179
                     /gene="phnX"
                     /locus_tag="ACG8H8_001919"
     CDS             5394..6179
                     /gene="phnX"
                     /locus_tag="ACG8H8_001919"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP8986981.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6525..8216)
                     /gene="argS"
                     /locus_tag="ACG8H8_001920"
     CDS             complement(6525..8216)
                     /gene="argS"
                     /locus_tag="ACG8H8_001920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP8986982.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8638..9585)
                     /gene="arcC"
                     /locus_tag="ACG8H8_001921"
     CDS             complement(8638..9585)
                     /gene="arcC"
                     /locus_tag="ACG8H8_001921"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP8986983.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9700..10719)
                     /gene="argF"
                     /locus_tag="ACG8H8_001922"
     CDS             complement(9700..10719)
                     /gene="argF"
                     /locus_tag="ACG8H8_001922"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP8986984.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10810..12039)
                     /gene="arcA"
                     /locus_tag="ACG8H8_001923"
     CDS             complement(10810..12039)
                     /gene="arcA"
                     /locus_tag="ACG8H8_001923"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP8986985.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3346 a         2535 c         2192 g         4112 t
ORIGIN      
        1 aatttttaaa taaaagaaat ataaataaaa acagaggaag cggaatgatc cgcccctctg
       61 tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc gatcttatct
      121 acttctttaa tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca
      181 tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg
      241 atgttacgaa tcgcgatttt tgcattttct gcttcttttt tcacatcttt agctaattct
      301 tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt
      361 ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg
      421 tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc
      481 aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca
      541 cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt
      601 tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga
      661 tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca
      721 gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt
      781 tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg
      841 gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga
      901 tttctgccgc tcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc
      961 ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg
     1021 gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt
     1081 ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag
     1141 cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccac
     1201 ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt
     1261 ctttgattac tggtggtttg attccaaatc ctgcatcccc agctaaagct tctccactca
     1321 attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta
     1381 ttgccatttt accatactta gatgatttcg acactctaag actggttttt cttgttttgc
     1441 ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt
     1501 tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa
     1561 cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag
     1621 tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct
     1681 tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt
     1741 gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta
     1801 cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag
     1861 ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa
     1921 atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga
     1981 taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca
     2041 ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta
     2101 aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg
     2161 ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt
     2221 tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc
     2281 cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta
     2341 gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct
     2401 ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc
     2461 catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc
     2521 gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg
     2581 gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc
     2641 atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat
     2701 agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg
     2761 gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt
     2821 ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa
     2881 agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt
     2941 atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg
     3001 agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac
     3061 gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt
     3121 tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt
     3181 tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat
     3241 agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc
     3301 taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc
     3361 tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat
     3421 atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac
     3481 agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa
     3541 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
     3601 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
     3661 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
     3721 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
     3781 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
     3841 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
     3901 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
     3961 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
     4021 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
     4081 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
     4141 tattttcctt attaaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
     4201 ttttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
     4261 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
     4321 attaactcct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
     4381 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
     4441 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcggctcctt
     4501 tggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     4561 aaacggcgca tatggacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     4621 cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga
     4681 agagaatccc tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     4741 gaataatttg gaagagattg ctgctgtcac acaagaaaaa gatcttttgt ttatagtgga
     4801 cgcgatgtca agctttggtg ggataccgat tcgtgtagcc gaactaggga ttgatttttt
     4861 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     4921 taaagcggaa ctagaaaagc gaaaaaatgt gagtcgaagt ttgtcacttg atctatatga
     4981 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     5041 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     5101 atattcacgt tatctcgcca ataatcatgt actacgacaa aaaatgacag agatggggtt
     5161 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     5221 ctggcgattt gattttaatc atttttatca gtatttaaag caaaacggat ttgtgatcta
     5281 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     5341 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     5401 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     5461 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgcg
     5521 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     5581 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     5641 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     5701 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgactg
     5761 gatttactgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa gggtattcac
     5821 cagacttcat ggcaacttct gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     5881 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     5941 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     6001 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     6061 aagatcgttt aaaacagtct gttgctctta gattccaaca agctggcgct gatatttgtg
     6121 taaacaattt aaaagagtta acagaataca ttttgtacgc tgacaagaat ccagaatgac
     6181 ttttatacaa aatcagaggt cgtgaaaaag ctgtactgca tcaagtaata agaacagcca
     6241 aacaaaaata gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt
     6301 ttgaacaccg tttattcgaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     6361 ttatttacaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     6421 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     6481 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     6541 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     6601 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     6661 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     6721 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     6781 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     6841 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     6901 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     6961 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     7021 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     7081 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     7141 tggcgcatat cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     7201 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     7261 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     7321 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     7381 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     7441 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     7501 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttc atcgccttct
     7561 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     7621 tcatggaatt ggacatataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     7681 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     7741 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     7801 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     7861 atgtcgattg gtactgttcc ttgattcccg atattgctat ccccataatg ttctttttct
     7921 ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     7981 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     8041 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     8101 acatctccgt gttcagctga ttttggattt tctagtaatt gttcgacttg ttccagtgtt
     8161 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     8221 attcctccta aagtgctccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     8281 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     8341 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     8401 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     8461 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     8521 aaaaaaaaac gaaaggctag tcgtttctga taaaaaaaca atccagatgg cctaaaggag
     8581 aagaaatacc acctggatag ctataaatca tttcgtaaat acgaaagact taacaaatta
     8641 atcagctacg ataatcgttc cttcttcaga agctaaaaga ttctcgatat tttctaaaga
     8701 agtgatgatt gccttagcgt ttggttttgc ttcaacaaat gcaatagctg cctctacttt
     8761 cggaagcatg cttcctggag caaattgctt ttcatcaatg tattgtttca tttctgaaac
     8821 agtaacagtt tctaatttct tttgatctgg tttttggtaa ttgacataca cattgtctac
     8881 tcctgtcaat acgatcagca aatccgcatc gatgatttcg gctaattttt ctgaagcaaa
     8941 atctttatcg attacggctt ctcgtccttc taatccagta gctgtttcta caacaggaat
     9001 cccaccgcca ccaacagaga ctgtgataac tccttgatcg accagcgttt cgatgacacg
     9061 tgcttcttta atcgagatgg gtttagggga agcgactact tttctccatc cacgtccagc
     9121 atcttctttg aatgtcacag tggagtcagc attcatttgt tctttggctt cttcttccgt
     9181 gtagaatgga ccgactggtt tacttggatt tttgaatgaa ggatcatttt catccacaat
     9241 aacttgagtg actaaagaaa ccacatcttt atcgatccct ttttccttca gtacttcgcc
     9301 catggcattt tgtaaccagt aaccgataga tccttctgtc attgcaacac atgtatctaa
     9361 aggcattgcc ggtgttttct cagaatcagc agcttgctgc tgaatcaata ggttaccgac
     9421 ttgcggacca tttccgtggg agatgatcaa ttcatctcct tgttcgataa atttcactaa
     9481 atactttgct gtttccatta acgcttcttg ttgggctttt gcacttgcat ccgtagacaa
     9541 gattgcatta ccccctaaag cgacaactac ttttcgtttg gccatttcat tcgtcctttc
     9601 ttcagttcta ccgaattttc tagataacaa aacgagccag cgttttgtga ttttccctca
     9661 atgctgactc gttttttcgt gagatgaatg tggttatgct taaacgcgag ggataaacaa
     9721 gttacctaat gttgctgcca tgatcgcttt gattgagtgc atacgatttt ctgcttggtc
     9781 aaattgacga gcatatttgc tgcggaagac ttcatctgtt acttccattt cagtgatgcc
     9841 aaagcgttct ttcatttgtt caccgtaaac agtgtttgta tcatggaaag ctggcaagca
     9901 gtgcaagaag atcaaacgat ctgtgttgtg tgttttttct accatttcca tattgatttg
     9961 gtaaggtttc aacaatttga tacgttcttc gaatttatct tcttcaccca ttgataccca
    10021 tacatcagaa taaaggacgt ctgctccgtc tacaccttta tcaacatcgt cggtaatcat
    10081 caattgtgca ccggattttt cagcaaagcc ttctgccatt ttgacgattt cttcttcagg
    10141 ttgtaattct tttggtgcaa cgatacgcat attcgctcct aaaattgcac ctgtgactaa
    10201 taaagagtta gccatattgt ttcgaccatc tccgcagtaa gcaacagtga taccttctac
    10261 tgttccaaag ttttcttgga tagttaaaaa gtcagcgatc atttgtgttg gatgccattc
    10321 atcagtcaag ccattccaaa ctggaacacc agagaattct gctagttcct ctaccatttt
    10381 ttgactgaat ccacggaatt cgattccatc aaacatacga cctagtactt tagctgtatc
    10441 ttctgtggat tcttttttac ctaattggat atcatttgca cctagatatt caggatgtgc
    10501 acctagatcg atcgctgcag tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa
    10561 taaaagcgca atattttttc cttctagata gtgatgtggg ataccacgtt ttttcaagtc
    10621 tttcaagtgt tctgaaaaat cgatcaaata ttgtaattct tcttttgtaa agtctttttc
    10681 tgctaataag cttcttcctt ggaatactga ttctttcata ttaaacattc tcctttagat
    10741 tgtttttatt taagattaaa gaggaaatcc cgacggcagc tgacagtaag accgccgtca
    10801 ggaaacaaat tacttcaagt cttcacggac aagtggttgg ctcatgcagc gtggaccacc
    10861 acggcctcgt gacaattcac ttgaattgat ttctaacact ttgatgccat agctacgaag
    10921 caattcgttc gatacataat ttcggttata agtgactaca acacctggag cgattgccaa
    10981 tgtgtttgat ccatcattcc attgttctcg aggtgcaacg atctcatctc cattaccagt
    11041 tgggatcagt acaagatcat ctaatccaag agcagctttc atcgtttcgt gtaagttatc
    11101 tgaatgagta atcttgatat catcaccatc tggttcgatt gtaaagacat ccatcttacc
    11161 gtctttactt tgaattgctg gatggatagt gaatttatcg tgatctacca ttgtaaatac
    11221 ggtatccaaa tgcatcattg cacggttgtt tggaattttg attgctaaca ctttttcaaa
    11281 tcctgaattt tttgcgaata ggtggcgtgc taatttttct aaagctttcg cattagttcg
    11341 ttgagaaatc ccaacagcta ctactttatc gcttaagatc aattcgtctc cgccttcgat
    11401 atgatctgga ttttcacgat ccaaccatac ttcgattcct ttatttgcaa atcttggatg
    11461 atgtttcaaa atatattctg taaacatcga ttcacgacga cgtgcttcaa aagtcatctt
    11521 attgactgtc atcccatttc ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg
    11581 cattgggtcc atgtaaaatg gatagtcatc gtctgctgac agatcgtata gactgctaga
    11641 acgtacttca atatcttttg tacgaacccc agccatgatt ttatctacca tttcttgcgt
    11701 ttccattgat agaaggaact catgaagacc ctctcttaca gcattcgatg caatatgaga
    11761 ttcatccaac attttattca agaattgttc tttgacattg cctgcgtcaa tcgcttctgc
    11821 cgctaatttt tccaagtaaa gagtctcgac gccttcattt tgcaatgtct ttgcaaagtt
    11881 gtcatgttct tcttgagcaa tcggaagata tggaatatca tcgaacaaca gacgatccat
    11941 aatatctggt gtcaaatttt ccacttcttg tccaggcctt ttcagtaaaa ctgtctttaa
    12001 tttccctatt tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt
    12061 tgattacatg tataggatat cctccttttg taatcacttt cataattttg tctcattcaa
    12121 aatgagacat tcctcacatt aatattcgat aattaaataa ttaaacgttt ttctattttt
    12181 aagcg
//
LOCUS       DAZKAO010000082        12169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000082 DAZKAO010000000
VERSION     DAZKAO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.082"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..300)
                     /locus_tag="ACG8H8_001924"
     CDS             complement(136..300)
                     /locus_tag="ACG8H8_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986986.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            595..1923
                     /locus_tag="ACG8H8_001925"
     CDS             595..1923
                     /locus_tag="ACG8H8_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP8986987.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="ACG8H8_001926"
     CDS             2125..2958
                     /locus_tag="ACG8H8_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP8986988.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..3711
                     /locus_tag="ACG8H8_001927"
     CDS             2974..3711
                     /locus_tag="ACG8H8_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP8986989.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3866..4834)
                     /locus_tag="ACG8H8_001928"
     CDS             complement(3866..4834)
                     /locus_tag="ACG8H8_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP8986990.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5066..5899
                     /locus_tag="ACG8H8_001929"
     CDS             5066..5899
                     /locus_tag="ACG8H8_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP8986991.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5933..6772
                     /locus_tag="ACG8H8_001930"
     CDS             5933..6772
                     /locus_tag="ACG8H8_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8986992.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6798..7353
                     /locus_tag="ACG8H8_001931"
                     /pseudo
     CDS             6798..7353
                     /locus_tag="ACG8H8_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            7552..9273
                     /locus_tag="ACG8H8_001932"
     CDS             7552..9273
                     /locus_tag="ACG8H8_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP8986993.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9438..10580
                     /locus_tag="ACG8H8_001933"
     CDS             9438..10580
                     /locus_tag="ACG8H8_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP8986994.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10596..11807
                     /gene="thiI"
                     /locus_tag="ACG8H8_001934"
     CDS             10596..11807
                     /gene="thiI"
                     /locus_tag="ACG8H8_001934"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP8986995.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4149 a         2041 c         2527 g         3452 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     3721 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     3781 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     3841 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     3901 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     3961 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     4021 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     4081 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     4141 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     4201 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     4261 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     4321 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     4381 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     4441 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     4501 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     4561 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     4621 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     4681 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     4741 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     4801 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     4861 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     4921 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     4981 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     5041 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     5101 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     5161 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     5221 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     5281 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     5341 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     5401 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     5461 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     5521 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     5581 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     5641 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     5701 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     5761 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     5821 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     5881 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     5941 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     6001 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     6061 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     6121 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     6181 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     6241 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     6301 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     6361 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     6421 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     6481 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     6541 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     6601 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     6661 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     6721 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     6781 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     6841 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     6901 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     6961 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     7021 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     7081 cagatatcct ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc
     7141 gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac
     7201 cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct
     7261 aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct
     7321 gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt
     7381 tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc
     7441 tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca
     7501 gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat
     7561 aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt
     7621 agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa
     7681 gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt
     7741 gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc
     7801 tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg
     7861 ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa
     7921 caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca
     7981 ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac
     8041 gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa
     8101 gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt
     8161 ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg
     8221 ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac
     8281 gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca
     8341 aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg
     8401 gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa
     8461 cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc
     8521 aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc
     8581 tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa
     8641 atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca
     8701 gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa
     8761 gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag
     8821 atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg
     8881 cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa
     8941 ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct
     9001 ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt agatgcagca
     9061 gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg
     9121 agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca
     9181 ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa
     9241 gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga
     9301 gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt
     9361 gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca
     9421 aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc
     9481 aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc
     9541 atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt
     9601 tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact
     9661 gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag
     9721 gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag
     9781 taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa
     9841 taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc
     9901 agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag
     9961 atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt
    10021 ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga
    10081 aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa
    10141 gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag
    10201 agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt
    10261 tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt
    10321 tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac
    10381 aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat
    10441 tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg
    10501 aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga
    10561 aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc
    10621 gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac
    10681 aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc
    10741 ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa
    10801 tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga
    10861 aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta
    10921 tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag
    10981 gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc
    11041 cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag
    11101 ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg
    11161 attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt
    11221 ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca
    11281 aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag
    11341 aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt
    11401 tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat
    11461 cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga
    11521 atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag
    11581 aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg
    11641 ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt
    11701 tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag
    11761 cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa
    11821 tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc
    11881 gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa
    11941 aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt
    12001 gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc
    12061 agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg
    12121 gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DAZKAO010000083        11669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000083 DAZKAO010000000
VERSION     DAZKAO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.083"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..540
                     /locus_tag="ACG8H8_001935"
     CDS             <1..540
                     /locus_tag="ACG8H8_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986996.1"
                     /translation="IVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLS
                     ILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHR
                     CSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEY
                     AFSFAREDEDNPKTYDILA"
     gene            complement(601..966)
                     /locus_tag="ACG8H8_001936"
     CDS             complement(601..966)
                     /locus_tag="ACG8H8_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP8986997.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1042..1287)
                     /locus_tag="ACG8H8_001937"
     CDS             complement(1042..1287)
                     /locus_tag="ACG8H8_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8986998.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1357..1611)
                     /locus_tag="ACG8H8_001938"
     CDS             complement(1357..1611)
                     /locus_tag="ACG8H8_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8986999.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1775..2098)
                     /locus_tag="ACG8H8_001939"
     CDS             complement(1775..2098)
                     /locus_tag="ACG8H8_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987000.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2285..2358
                     /locus_tag="ACG8H8_001940"
     tRNA            2285..2358
                     /locus_tag="ACG8H8_001940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2319..2321,aa:Ile,seq:cat)
     gene            complement(2743..3240)
                     /locus_tag="ACG8H8_001941"
     CDS             complement(2743..3240)
                     /locus_tag="ACG8H8_001941"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP8987001.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3280..4089)
                     /locus_tag="ACG8H8_001942"
     CDS             complement(3280..4089)
                     /locus_tag="ACG8H8_001942"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8987002.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4423..5280
                     /locus_tag="ACG8H8_001943"
     CDS             4423..5280
                     /locus_tag="ACG8H8_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP8987003.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5273..6175
                     /locus_tag="ACG8H8_001944"
     CDS             5273..6175
                     /locus_tag="ACG8H8_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP8987004.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6208..6558
                     /locus_tag="ACG8H8_001945"
     CDS             6208..6558
                     /locus_tag="ACG8H8_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8987005.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6607..7530
                     /locus_tag="ACG8H8_001946"
     CDS             6607..7530
                     /locus_tag="ACG8H8_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8987006.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7608..8501
                     /locus_tag="ACG8H8_001947"
     CDS             7608..8501
                     /locus_tag="ACG8H8_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8987007.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8539..9102
                     /gene="amaP"
                     /locus_tag="ACG8H8_001948"
     CDS             8539..9102
                     /gene="amaP"
                     /locus_tag="ACG8H8_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP8987008.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9116..9304
                     /locus_tag="ACG8H8_001949"
     CDS             9116..9304
                     /locus_tag="ACG8H8_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP8987009.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9317..10219
                     /locus_tag="ACG8H8_001950"
     CDS             9317..10219
                     /locus_tag="ACG8H8_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8987010.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10251..10493
                     /locus_tag="ACG8H8_001951"
     CDS             10251..10493
                     /locus_tag="ACG8H8_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8987011.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10749..11120)
                     /locus_tag="ACG8H8_001952"
     CDS             complement(10749..11120)
                     /locus_tag="ACG8H8_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987012.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4157 a         1984 c         2205 g         3323 t
ORIGIN      
        1 atagttataa gcttttttaa agaatattta gaaattatta attttcgtct gaaaacaaaa
       61 aaagaaagcg ttattatttt agtctatttt caggggggga accttatgat aaaaaaacta
      121 tttttactat caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact
      181 gaactgatca caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac
      241 agtgactaca gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag
      301 catcgatgta gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc
      361 aaaagaagca atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac
      421 cacgtcagga gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac
      481 gcttttagtt ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga
      541 tctaagatgg ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata
      601 ttaggtaaga taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc
      661 atcgtgttcc ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc
      721 gcatgatttg ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt
      781 ttccatttca tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa
      841 aacataatcc acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc
      901 ccgatgttgt cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa
      961 tcgcatagaa atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac
     1021 cagcaccaag gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag
     1081 gtaatcaacg gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat
     1141 ttcttctcct tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac
     1201 agctacttga gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc
     1261 cagtaaaata gcttcttcga tactcattta atcacctttc attatagtag aatagttata
     1321 atgactatag aaagctacac atgcaatcaa tagtactcaa tgttttttat ttaaacgtat
     1381 caaataaaga atgacgatca ataaagacaa aaccagtcct gataattctt ctataaaaac
     1441 actatcgggt tcaaagtagt tgtaattaaa aactttccac ataaagtaga tagccggaag
     1501 agtcaaaata gctaaataaa accgttcttt gttccaattc ataagataaa caatacttgc
     1561 gaccagtgcg accgcaggca cccaaaacaa aactctcacc aacgtaatca tgtaacggac
     1621 tcctcaacga ttcatataat aaaagataac atgttattgc atacatcaca aatgaaatgc
     1681 taggaaaata aaagaagaat agatcaatga aaaatgaaaa agcagaacag ttttctgctg
     1741 ataagcagga actgttccgc tgaatgtgtc ttatctatcg agaaaaaatc aatggattga
     1801 tatctaagta attcaataca tataaaaaga ggacgatcgc attcactaaa aaggaaagaa
     1861 tgatgatccc atagcgaatg gaaacatatt taacaaatac ttttgtttga atacgggcat
     1921 atctttttcc tttatagctg agcatcatga caagaagcaa aagggtaatc acaccttgaa
     1981 aaatcattcc aataaacaga attcctgagg tttgataatt taattggaga aaataagctg
     2041 ctgccgcaac taaatcgaat aaaatcgcaa taaatggcaa aataatcact cctaacattg
     2101 agaataaata aaagaataac attttttcta aaataagcaa ataaaaatta aagtaacaaa
     2161 atagttatga cacaagtaaa agaaataaga ataacaatat tattttactt atattttgaa
     2221 acttgtgaga gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa attttattta
     2281 tgatgtacct ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt
     2341 cgagtcctac aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     2401 cagccaagta aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca
     2461 agcttttgaa taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag
     2521 cttaacaaac ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt
     2581 cagctgattg aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat
     2641 aaaaaagcca atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt
     2701 tttttattgt gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga
     2761 gacgttgaaa tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa
     2821 cattggatca acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga
     2881 gagtacacac aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct
     2941 ttcaaataaa gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt
     3001 tttaatcact cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg
     3061 ttcttcttct ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt
     3121 attttggcgt gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg
     3181 aatataaaat tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat
     3241 tttctttcct cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat
     3301 ataagcaaca ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg
     3361 tcgctgtgca ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt
     3421 ttcttgatcg ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa
     3481 aacaagtgaa tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga
     3541 gaatactgat tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc
     3601 actcttatct gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa
     3661 aatttgatca aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac
     3721 tagatttgat tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga
     3781 tattttcgct tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga
     3841 acgtctgaga actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga
     3901 agaaatcaaa tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt
     3961 ccttccggta gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat
     4021 attttcttct ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc
     4081 tagtttcatt ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat
     4141 taaatgagaa tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa
     4201 acattcttaa gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa
     4261 agccatgaaa aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa
     4321 atattcaatg aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat
     4381 gtagaacagg ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc
     4441 gtagttcctg tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc
     4501 gcaactgcat tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat
     4561 ggtctatcag aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat
     4621 gcaagtatcc aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac
     4681 catatcacaa ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat
     4741 tataagaaag tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta
     4801 tatgatgagc cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa
     4861 gctttggaaa gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg
     4921 attgacattg atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt
     4981 gaaaatggcg atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa
     5041 gattgggaac aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat
     5101 ccagcaccaa atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc
     5161 gttcacttta caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat
     5221 ttatacttaa aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa
     5281 aaaagaaata gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt
     5341 atttgtctca gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga
     5401 cgatgatatc gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat
     5461 gaactcagat gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag
     5521 cgatcgtatt ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac
     5581 agaagttgaa cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa
     5641 attatggcgt cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca
     5701 tcaacgactg gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt
     5761 gatgctgatc aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt
     5821 catcgaacat aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt
     5881 acatgaccgt tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa
     5941 agcaaaaaaa catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc
     6001 ttacattatc catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac
     6061 gaagaaatat tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc
     6121 taaatatcca aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa
     6181 gacaacaact gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag
     6241 acgttaattg aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc
     6301 gatccatttc taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct
     6361 tttgtttcgg aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat
     6421 acatctaaat tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag
     6481 cttgacagct tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag
     6541 aactgcttct tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac
     6601 tactttgtgg atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc
     6661 gtacttgata gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct
     6721 gatgatattt tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat
     6781 gagacgaaac tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat
     6841 cgtatcgaaa aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact
     6901 tgtatcgata gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta
     6961 aactcgtttg aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa
     7021 gaactaagag agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg
     7081 gaaaatgaag aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat
     7141 accttaagag atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa
     7201 cgtttagacg atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa
     7261 atgatcgaga tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg
     7321 gacaataatc taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt
     7381 ccggcgttgt tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct
     7441 gctctaggct ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac
     7501 catttatacc ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa
     7561 gaaatttgtt ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa
     7621 aattaatcga cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata
     7681 caccagcttt acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag
     7741 gcaataatca actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc
     7801 gtgcagcagc gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag
     7861 gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa agatgcagga cgtaagattg
     7921 tcttactacc ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg
     7981 tgagtgaact tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc
     8041 agtctattgc tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg
     8101 ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa
     8161 caacaacttc tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa
     8221 aagcttcaat cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc
     8281 gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt acaactagac gaaggacaac
     8341 cttctgattc gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag
     8401 gagaattagc accagtttat gttttcttag catcgaataa agcaagttat gttacagccc
     8461 aaatttacgg agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc
     8521 ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta
     8581 cttttgctat ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt
     8641 cagctagaga agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt
     8701 tggcttgccg ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat
     8761 ccaaaaacaa tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa
     8821 aaagcaattg aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca
     8881 aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa
     8941 cgaacttcag cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta
     9001 caacacatat tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa
     9061 gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa
     9121 agaactattt tcgacataca agctccctat catctttggg gtacttggat tgatattagc
     9181 cgttttatgg atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat
     9241 cggcgtcgct atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc
     9301 ttaggaggaa acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca
     9361 aaaaccagtg tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga
     9421 tgaaaagaac aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca
     9481 acaaggagta gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac
     9541 tccagaatct aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga
     9601 gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa
     9661 cggacaagga aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca
     9721 ggaagattca atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg
     9781 aatcgcaatt gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt
     9841 ggcaggaaaa cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa
     9901 aaaacaagta gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat
     9961 ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt
    10021 gatcgaagtc aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca
    10081 agaaacggtg caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa
    10141 tcaaacagat aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac
    10201 agaaccacgg gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta
    10261 tttggtcact tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag
    10321 gttcttcgat ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc
    10381 aagcattatt aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga
    10441 ttggtgccat cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact
    10501 tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa
    10561 atagcttctc atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca
    10621 ctgtttatac ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg
    10681 gcaaattccc aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag
    10741 aagcttcttt aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata
    10801 caaaataata cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga
    10861 tgaagtaaaa agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa
    10921 tatctctttt ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa
    10981 acaaatagaa gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga
    11041 cgattgacga gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa
    11101 aagtaaacaa taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa
    11161 ataacgatat ttgtttgtga agctttttcg tgtcgtaaca aatgttatct aatgtatttt
    11221 taaataaata atcattatta tcaaaataat gttaaaaata aaaaagaatt gtccatcact
    11281 gttattcaaa tcattttatc aaaactacat agtaaatgtt aaaaagcaac atgacataat
    11341 atatagaagg ctttacagta tatatgggag cttactaaaa aagaatggag taagagttaa
    11401 cacaatgggc cattagctga agttttaagc acttcaaggt atgaaacaga atggcctgta
    11461 atactacatg attatttaga ctgctcaaac tgctatcagt ctggtggaat gaataaagta
    11521 ttatactaaa agcagatcca actacaacgt aacaggaaac cagttgtatc aggaaaagtg
    11581 atagtacatg gagaagaaaa ggcccccttt gtatgagtca gtacatatct tatttataga
    11641 taaaacggta ttcaatcgaa ataaaaaat
//
LOCUS       DAZKAO010000084        11545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000084 DAZKAO010000000
VERSION     DAZKAO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.084"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..330)
                     /locus_tag="ACG8H8_001953"
     CDS             complement(1..330)
                     /locus_tag="ACG8H8_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP8987013.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="ACG8H8_001954"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="ACG8H8_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP8987014.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="ACG8H8_001955"
     CDS             1251..2264
                     /locus_tag="ACG8H8_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8987015.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="ACG8H8_001956"
     CDS             2264..3541
                     /locus_tag="ACG8H8_001956"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8987016.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="ACG8H8_001957"
     CDS             3604..6306
                     /locus_tag="ACG8H8_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8987017.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="ACG8H8_001958"
     CDS             6458..6775
                     /locus_tag="ACG8H8_001958"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8987018.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="ACG8H8_001959"
     CDS             6805..7125
                     /locus_tag="ACG8H8_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8987019.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="ACG8H8_001960"
     CDS             7233..8693
                     /locus_tag="ACG8H8_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8987020.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="ACG8H8_001961"
     CDS             8761..8982
                     /locus_tag="ACG8H8_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987021.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="ACG8H8_001962"
     CDS             9013..9195
                     /locus_tag="ACG8H8_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP8987022.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="ACG8H8_001963"
     CDS             9195..9608
                     /locus_tag="ACG8H8_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP8987023.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="ACG8H8_001964"
     CDS             9731..10912
                     /locus_tag="ACG8H8_001964"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987024.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10983..11147)
                     /locus_tag="ACG8H8_001965"
     CDS             complement(10983..11147)
                     /locus_tag="ACG8H8_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987025.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11301..11374
                     /locus_tag="ACG8H8_001966"
     tRNA            11301..11374
                     /locus_tag="ACG8H8_001966"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11443..>11545)
                     /locus_tag="ACG8H8_001967"
     CDS             complement(11443..>11545)
                     /locus_tag="ACG8H8_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP8987026.1"
                     /translation="TTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         3793 a         1950 c         2435 g         3367 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    11401 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    11461 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    11521 atgaagataa gtagacattg tagtt
//
LOCUS       DAZKAO010000085        11534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000085 DAZKAO010000000
VERSION     DAZKAO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.085"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..457)
                     /gene="comGG"
                     /locus_tag="ACG8H8_001968"
     CDS             complement(98..457)
                     /gene="comGG"
                     /locus_tag="ACG8H8_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP8987027.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(429..854)
                     /gene="comGF"
                     /locus_tag="ACG8H8_001969"
     CDS             complement(429..854)
                     /gene="comGF"
                     /locus_tag="ACG8H8_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP8987028.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(817..1164)
                     /locus_tag="ACG8H8_001970"
     CDS             complement(817..1164)
                     /locus_tag="ACG8H8_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987029.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(1136..1606)
                     /gene="comGD"
                     /locus_tag="ACG8H8_001971"
     CDS             complement(1136..1606)
                     /gene="comGD"
                     /locus_tag="ACG8H8_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP8987030.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1581..1859)
                     /gene="comGC"
                     /locus_tag="ACG8H8_001972"
     CDS             complement(1581..1859)
                     /gene="comGC"
                     /locus_tag="ACG8H8_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP8987031.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1856..2905)
                     /gene="comGB"
                     /locus_tag="ACG8H8_001973"
     CDS             complement(1856..2905)
                     /gene="comGB"
                     /locus_tag="ACG8H8_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP8987032.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2862..3791)
                     /gene="comGA"
                     /locus_tag="ACG8H8_001974"
     CDS             complement(2862..3791)
                     /gene="comGA"
                     /locus_tag="ACG8H8_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP8987033.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3923..5677
                     /gene="ade"
                     /locus_tag="ACG8H8_001975"
     CDS             3923..5677
                     /gene="ade"
                     /locus_tag="ACG8H8_001975"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP8987034.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(5775..6437)
                     /gene="trmB"
                     /locus_tag="ACG8H8_001976"
     CDS             complement(5775..6437)
                     /gene="trmB"
                     /locus_tag="ACG8H8_001976"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP8987035.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(6507..7289)
                     /locus_tag="ACG8H8_001977"
     CDS             complement(6507..7289)
                     /locus_tag="ACG8H8_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP8987036.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(7493..8704)
                     /locus_tag="ACG8H8_001978"
     CDS             complement(7493..8704)
                     /locus_tag="ACG8H8_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987037.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(8697..9434)
                     /locus_tag="ACG8H8_001979"
     CDS             complement(8697..9434)
                     /locus_tag="ACG8H8_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987038.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9572..10006
                     /locus_tag="ACG8H8_001980"
     CDS             9572..10006
                     /locus_tag="ACG8H8_001980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP8987039.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            9999..10343
                     /locus_tag="ACG8H8_001981"
     CDS             9999..10343
                     /locus_tag="ACG8H8_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP8987040.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            10523..11533
                     /locus_tag="ACG8H8_001982"
     CDS             10523..11533
                     /locus_tag="ACG8H8_001982"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8987041.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         3739 a         2328 c         1969 g         3498 t
ORIGIN      
        1 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
       61 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
      121 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
      181 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
      241 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
      301 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
      361 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
      421 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
      481 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
      541 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
      601 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
      661 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
      721 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
      781 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
      841 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
      901 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
      961 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     1021 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     1081 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     1141 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     1201 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     1261 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     1321 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     1381 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     1441 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     1501 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     1561 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     1621 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     1681 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     1741 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     1801 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     1861 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     1921 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     1981 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     2041 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     2101 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     2161 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     2221 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     2281 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     2341 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     2401 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     2461 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     2521 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     2581 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     2641 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     2701 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     2761 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     2821 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     2881 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     2941 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     3001 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     3061 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     3121 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     3181 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     3241 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     3301 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     3361 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     3421 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     3481 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     3541 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     3601 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     3661 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     3721 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     3781 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     3841 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     3901 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     3961 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     4021 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     4081 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     4141 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     4201 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     4261 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     4321 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     4381 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     4441 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     4501 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     4561 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     4621 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     4681 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     4741 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     4801 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     4861 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     4921 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     4981 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     5041 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     5101 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     5161 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     5221 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     5281 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     5341 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     5401 tgtcaacaat cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     5461 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     5521 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     5581 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     5641 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     5701 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     5761 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     5821 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     5881 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     5941 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     6001 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     6061 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     6121 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     6181 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     6241 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     6301 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     6361 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     6421 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     6481 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     6541 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     6601 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     6661 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     6721 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     6781 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     6841 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     6901 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     6961 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     7021 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     7081 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     7141 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     7201 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     7261 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     7321 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     7381 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     7441 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     7501 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     7561 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     7621 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
     7681 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
     7741 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
     7801 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
     7861 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
     7921 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
     7981 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
     8041 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
     8101 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
     8161 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
     8221 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
     8281 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
     8341 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
     8401 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
     8461 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
     8521 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
     8581 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
     8641 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
     8701 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
     8761 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
     8821 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
     8881 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
     8941 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
     9001 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
     9061 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
     9121 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
     9181 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
     9241 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
     9301 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
     9361 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
     9421 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
     9481 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
     9541 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
     9601 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
     9661 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
     9721 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
     9781 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
     9841 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
     9901 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
     9961 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    10021 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    10081 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    10141 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    10201 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    10261 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    10321 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    10381 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    10441 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    10501 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    10561 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    10621 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    10681 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    10741 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    10801 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    10861 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    10921 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    10981 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    11041 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    11101 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    11161 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    11221 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    11281 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    11341 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    11401 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    11461 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    11521 ttcatcaaaa taaa
//
LOCUS       DAZKAO010000086        11504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000086 DAZKAO010000000
VERSION     DAZKAO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.086"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8H8_001983"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8H8_001983"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP8987042.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG8H8_001984"
     CDS             complement(2262..2513)
                     /locus_tag="ACG8H8_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP8987043.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8H8_001985"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8H8_001985"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP8987044.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACG8H8_001986"
     CDS             3463..3681
                     /locus_tag="ACG8H8_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987045.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACG8H8_001987"
     CDS             complement(3970..4482)
                     /locus_tag="ACG8H8_001987"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP8987046.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8H8_001988"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8H8_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP8987047.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACG8H8_001989"
     CDS             complement(6935..7378)
                     /locus_tag="ACG8H8_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP8987048.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACG8H8_001990"
     CDS             complement(7379..8167)
                     /locus_tag="ACG8H8_001990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8987049.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8H8_001991"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8H8_001991"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP8987050.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACG8H8_001992"
     CDS             complement(9282..9869)
                     /locus_tag="ACG8H8_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP8987051.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8H8_001993"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8H8_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP8987052.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         3129 a         2613 c         2025 g         3737 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctat
//
LOCUS       DAZKAO010000087        11254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000087 DAZKAO010000000
VERSION     DAZKAO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.087"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8H8_001994"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8H8_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP8987053.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8H8_001995"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8H8_001995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP8987054.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG8H8_001996"
     CDS             complement(2169..2906)
                     /locus_tag="ACG8H8_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP8987055.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG8H8_001997"
     CDS             complement(2997..3524)
                     /locus_tag="ACG8H8_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987056.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8H8_001998"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8H8_001998"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP8987057.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG8H8_001999"
     CDS             complement(4401..4886)
                     /locus_tag="ACG8H8_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP8987058.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACG8H8_002000"
     CDS             complement(5039..5236)
                     /locus_tag="ACG8H8_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987059.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8H8_002001"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8H8_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP8987060.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8H8_002002"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8H8_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP8987061.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACG8H8_002003"
     CDS             9211..9423
                     /locus_tag="ACG8H8_002003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP8987062.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACG8H8_002004"
     CDS             9425..11104
                     /locus_tag="ACG8H8_002004"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP8987063.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DAZKAO010000088        11230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000088 DAZKAO010000000
VERSION     DAZKAO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.088"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8H8_002005"
     CDS             6..305
                     /locus_tag="ACG8H8_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8987064.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8H8_002006"
     CDS             378..1739
                     /locus_tag="ACG8H8_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP8987065.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8H8_002007"
     CDS             1761..2021
                     /locus_tag="ACG8H8_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987066.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8H8_002008"
     CDS             2035..2787
                     /locus_tag="ACG8H8_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP8987067.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8H8_002009"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8H8_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8987068.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8H8_002010"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8H8_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP8987069.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8H8_002011"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8H8_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8987070.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8H8_002012"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8H8_002012"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP8987071.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8H8_002013"
     CDS             7161..8558
                     /locus_tag="ACG8H8_002013"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8987072.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8H8_002014"
     CDS             8639..9079
                     /locus_tag="ACG8H8_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8987073.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8H8_002015"
     CDS             9227..9553
                     /locus_tag="ACG8H8_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8987074.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8H8_002016"
     CDS             9554..10303
                     /locus_tag="ACG8H8_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP8987075.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8H8_002017"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8H8_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8987076.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3560 a         1961 c         2303 g         3406 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac
//
LOCUS       DAZKAO010000089        11200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000089 DAZKAO010000000
VERSION     DAZKAO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.089"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            323..1273
                     /gene="yidC"
                     /locus_tag="ACG8H8_002018"
     CDS             323..1273
                     /gene="yidC"
                     /locus_tag="ACG8H8_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP8987077.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            1377..2789
                     /locus_tag="ACG8H8_002019"
                     /pseudo
     CDS             1377..2789
                     /locus_tag="ACG8H8_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(2775..3120)
                     /locus_tag="ACG8H8_002020"
                     /pseudo
     CDS             complement(2775..3120)
                     /locus_tag="ACG8H8_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737849.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3120..3248)
                     /locus_tag="ACG8H8_002021"
                     /pseudo
     CDS             complement(3120..3248)
                     /locus_tag="ACG8H8_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(3394..3594)
                     /locus_tag="ACG8H8_002022"
     CDS             complement(3394..3594)
                     /locus_tag="ACG8H8_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987078.1"
                     /translation="MAEMHDHLLHSICERTGLSEEEVNQKLMEISEAMNKGVDFSMNQ
                     LDDFLAQHMKDALASDAADSVE"
     gene            complement(3944..4291)
                     /locus_tag="ACG8H8_002023"
     CDS             complement(3944..4291)
                     /locus_tag="ACG8H8_002023"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP8987079.1"
                     /translation="MDHLHDHDAENRLESNYARYLGGIEQTIEDSYTQDCMMSYTPDI
                     VEKIAPTDFKKMERKRLQEEIQKLTFNQRKVIEHYYFLNKNQQQIARDLRMSHAEITK
                     TLKRAFAVMKEVY"
     gene            complement(4485..4616)
                     /locus_tag="ACG8H8_002024"
     CDS             complement(4485..4616)
                     /locus_tag="ACG8H8_002024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987080.1"
                     /translation="MEDSKLPVTLIDVRRIINLGKQYMEGNQGQHQRRKAARLPLAE"
     gene            complement(4686..5063)
                     /locus_tag="ACG8H8_002025"
     CDS             complement(4686..5063)
                     /locus_tag="ACG8H8_002025"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987081.1"
                     /translation="MFFLAFLYLYIANVKYFSKWPLLLFWIISCVLLLVKNAVFQFLL
                     KKIGSKSHTRVLVVENGNLAKEYGLAMLSDPDADANIIGYVKDRKKEDILLEEPGFAN
                     YGDGLAKLKYFGRISDVQDVIVK"
     gene            complement(5087..5410)
                     /locus_tag="ACG8H8_002026"
     CDS             complement(5087..5410)
                     /locus_tag="ACG8H8_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987082.1"
                     /translation="MIDRRDDSDRCWSIRYYYKGRINEIIKERFWNTVFDCIGDAASI
                     FVAYLLAMFICYHVLEAAPGINMLSVPYLMMAALYSILIADFLRIFRATNKTYFRKYQ
                     NGFLK"
     gene            complement(5472..5948)
                     /locus_tag="ACG8H8_002027"
     CDS             complement(5472..5948)
                     /locus_tag="ACG8H8_002027"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFP8987083.1"
                     /translation="MSQIKFLVMDVDGTLTDGKIYMGECGEIMKAFNIKDGCAIHDIV
                     RAHNIEPIIITGRRSKILENRCKELGINILYQGIDNKIEKLLEITSDLSTVAYIGDDI
                     NDLSCMHPVKSAGGIVACPHDAVKKVLEISDFIAEHDGGNGAVREFVEWLVDTQNN"
     gene            complement(5965..6369)
                     /locus_tag="ACG8H8_002028"
     CDS             complement(5965..6369)
                     /locus_tag="ACG8H8_002028"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP8987084.1"
                     /translation="MSRSLYFLIKNSLLELEEGRYDLSNGIYLNVESYDIYRFEERMY
                     ESHLKYLDIQCILLGCEYIVVESISNLQIEVDYSADKDIVFYSNSIVGNCVLLEGGKL
                     LLLTPEDAHMPCIRVNESAKVKKAVFKISINY"
     gene            complement(6413..7378)
                     /locus_tag="ACG8H8_002029"
     CDS             complement(6413..7378)
                     /locus_tag="ACG8H8_002029"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFP8987085.1"
                     /translation="MDKIIEAKRVFDIEIEALKRTRDCLGKTFVRILDAIVNCEGKVI
                     ITGMGKPGHIAAKLAATFASLGTPAFRLHPAEAMHGDLGMVSEKDIVIAISYSGESDE
                     IVKILPNIKMIGATLIGITGNSKSTLATASDIVQILPEFEEACYLGLAPTSSATSVLC
                     YGDALAVVASGIYGFKDSDFGKFHPAGSLGKKLILKVSDLMSHGNEIPMVNSGTLLMD
                     AISVMSNKGLGIVSVIDQDGTLKGIITDGDLRRIIERKVDIYSVEIDDVMIANPKVFT
                     KDKLAVDALRFLKANSINILPIIDDEGMLVGTITWQQIVKAGIVA"
     gene            complement(7487..8263)
                     /gene="kdsA"
                     /locus_tag="ACG8H8_002030"
     CDS             complement(7487..8263)
                     /gene="kdsA"
                     /locus_tag="ACG8H8_002030"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP8987086.1"
                     /translation="MNRLMLIAGPCVIESEENVMLIAKTIKEITEKLDIDYYFKASFD
                     KANRTSITSYRGPGIEEGLKILKKVKETYGLKICTDIHEPWQAEKAAEVCDILQIPAF
                     LCRQTDLLVAAAKTGKIINVKKAQFLAPWDMKNVINKLVEAGNSNIMLCERGTSFGYN
                     TLVVDMTSIVEMKKFGYPVVMDATHSVQKPGGKGDVTGGNRNNVEPLAKAAVATGADA
                     LFFEVHPDPDNALSDGPNMVLLDEFEGLLERVLKVYNAVR"
     gene            complement(8265..9002)
                     /gene="kdsB"
                     /locus_tag="ACG8H8_002031"
     CDS             complement(8265..9002)
                     /gene="kdsB"
                     /locus_tag="ACG8H8_002031"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR00466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP8987087.1"
                     /translation="MKTIAIIPSRYGSSRFPGKPLAMISSKPMIQWVYENVSKAAMLD
                     AVYVATDDKRIFDCVESFGGMALMTSDKHTCGTDRLAECAEILQLRDEDLVLNIQGDE
                     PLIKAEMIADLYSCFDNDEVYMGTLKKHIEVQEELDNPNVVKVINDVNDYAIYFSRFC
                     VPYERDGKNSLHFKHIGVYGYKTWFLKKYSTMPKTPLEVSESLEQLRVLESGFKIRVK
                     ETKYQTVGVDTPEQIQQVEDELRKEGL"
     gene            complement(8999..10426)
                     /locus_tag="ACG8H8_002032"
     CDS             complement(8999..10426)
                     /locus_tag="ACG8H8_002032"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HFP8987088.1"
                     /translation="MNRLIKNYAYNLCYQILVMILPIITTPYVSRVLKAEGVGEFNYS
                     YAIVSVAVIIAQLGTNMYGQREIAYVQNDKIRRSGVFWSIFVIRIISSFIVFPVYILI
                     SATLHSYTVLLISMSVYLVANLFDVSWFFQGMEDFKKTAIRSMVVKLIGLVLIFLLVK
                     NENDVVLYSVILAGTELLGNALLCCHIPKYVRRISIHDISITPHIRPILGLFLPTAAV
                     YIYTYVNKIILGIFSTDVQVGLYSQPEKIVKLLMTVITSLGAVMLPHIAVAVKENNID
                     RIRKEVLNAVSFVFCIGAPMVVGAIIIANTFIPWFLGDGYAKSVSLFQLLSPLILIIG
                     FASVVGQAVLIPMKRQRVYTFSIMTGAVINLVVNLALIPHLEAIGAAIGTIFAELTVT
                     AIQFYEVRNCIELKMASIITNVKNYLIGALIMGGVGGYVSSKLPNSVISTFITVILCI
                     LLYSILLICMKDKLLMDYLKRRKNR"
     gene            complement(10428..>11200)
                     /locus_tag="ACG8H8_002033"
     CDS             complement(10428..>11200)
                     /locus_tag="ACG8H8_002033"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HFP8987089.1"
                     /translation="RVLSNIKYLFPTLMYILCMFISGNRVFLVKLFAIVFLIYIVMKS
                     DVSPRGRVPVMQVVRKTLFVAVAILAVFYALRGITKVNSTAATREFSDYITYYIGSPV
                     YMLDKYLQRYSPELNWGAKSLTSLVEMFGIEPEFKNAYIHVGGDSNFAGNEFSWFQRP
                     YSDFGWLGMLVFTFIVFWIFSWNIHIRINKEKNRRRRKYYILITAYFFYIPVMSFYYC
                     QMCFAFSITNIFSVVVACLLVREFTKRQDEIIFKIKEE"
BASE COUNT         3558 a         2412 c         1800 g         3430 t
ORIGIN      
        1 gataaggtta ttcttgaggc gctttgcctc aaaaattaag gacagcagtg aaaaagctga
       61 tctatcactt gcagtgagtt catttgactc cgctttttcg ggcagaaaaa aagtttttag
      121 tttattaaag taagaaagaa acacttggaa tcattctact ttttcctagt atttctttct
      181 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
      241 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
      301 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
      361 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
      421 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
      481 agttgaaaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
      541 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
      601 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
      661 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
      721 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
      781 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
      841 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcttatc tgcttcaagg
      901 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat
      961 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
     1021 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
     1081 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt
     1141 cactccaaga aaagatgtga cagaatcagc taaaccagct gacagcaaac aagcaaccaa
     1201 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
     1261 gaagcggaaa taaaaatata tgacaaaaaa ctgtagacaa ataaaaaaat ttctttgttc
     1321 tacagttttt ttgttacaat caaagaaaac gttcgctttt cgaggaggga tgtataatga
     1381 tgttcaaaca atcaatggat agtcgaatgg aagaagacaa aatttcgcta gatgcacttg
     1441 ttcctaaaaa tcatttagtc cgtaaaattg acaagttatt gtcatttgat tttatttatc
     1501 tcattgttga atcaacttat tcgaccatcg gtcgacctag cgttgatcct gtcgtcttag
     1561 taaaattagt attcattcaa tatttatttg ggattcgttc gatgcgtcag accattaaat
     1621 aagtttaaac aaacctagct tatcgttggt ttcttgggct tagtttaatt gataaagttc
     1681 ctcatttttc aacttttggt aaaaactatg cacggcgttt cagagaaacc gatttgtttg
     1741 aacagatatt tatcattatt ctggaacaag cagttgttgc tagatttatt catgaagaaa
     1801 ttttgtatat ggattcaact catattaaag agaatgcgaa aaaacgtatt tttacaaaag
     1861 atatggcaca taaagaagcc attttatacc aaaaacgatt ggaagaagaa atcaatgagg
     1921 caagaattgc cgaaggcaaa cgccattttg gttcaatcat tcgtcaggag gtggtcgaaa
     1981 ggaaatttag cgtcgcagat ccagaaattg gttattatgt aaaaacagaa cgagaaaaac
     2041 agtttgctta ttcttctcat actgtttgtg atgaaaatgg ttttgtatta gatgtcatga
     2101 ttacgcctag aaatctacat tacagccgaa tgctcgtgcc acaaatcgaa cgtgttaaat
     2161 cacgttatgg tgtagctgct gatgctgcgt acaaaacacc ttggaacgcc aagtatttaa
     2221 tcgaccgaaa actacgaccc acttttcctt atactcgacc caaaggaaac aaagaacgtt
     2281 ttaaaaagaa agattttgat tatgatccct attataactg gtacctatgc ccgaatgatc
     2341 aaacacttca gtttcgcaca actactcgag atggctataa aaaatatgta tctaaatctt
     2401 ttatttgtga aagttgtcca ttattgaaaa attatacaga aagccagaaa caccaaaaag
     2461 aaatacatcg acatatttgt gaagattact tagatgaagc agaacattta cgccatacgg
     2521 tgtacaatcg cgaaaaatat aaaaaaagaa acgattgaac gcatttttgc agacgcaaag
     2581 gaaaagcatg gagtgcgcta caccaagtat cgaggcttgg aaaaaatcag gtgcatacga
     2641 tgcttgtttt tgtcgcaatg aatctgaaaa aattagccac ttggcagtgg gagcatatgt
     2701 ctttatatct tttattatac aaaaatagac aaaagtatca gtctacactc ttagtcaaga
     2761 ggaaactgat gcttttatct acagtctgag agagtgggac aatagtcctt tctccaaaag
     2821 aaaacaagtc cgactttgat agttaatttt aaaatcggac ttgttttttc ttctttacgt
     2881 cttttcctct ccatgtattt ccctaatttt cttaaattta acgccatgag tgccaagcca
     2941 atgttaatct tggctccttg tttccctcgt aagtggaaat ccgtttccga tagatcgaac
     3001 cttctttttc ctctaaaaac tttttttaca ttctgctttg aaataacgcc atgtattatt
     3061 cactaaaatt tgtcggtttc gctcactttt tgctttcgta cattgactac gaacagggca
     3121 gcaccaccat aagccgcctg ccagttacct ctcactctta ccgggtcttt cagaacaccc
     3181 ggatgctcta acacatcact cggctaggct ccgttattga agaagaaagc cccatatctt
     3241 tccatggcat cctcggccag cttaagaagc tcctccatct gttcggagac cttcctcaca
     3301 tatatgggca aaggccatga agacctccac cagactgttc atgaccttct tcagtgctgc
     3361 gatcatctgc tctctcttct ccatggctta cacctactcg acagaatcag cggcatcaga
     3421 ggcaagagca tccttcatgt gctgtgccag gaagtcatcc agctggttca tagaaaaatc
     3481 gacacccttg ttcatggcct cgctgatttc catcagcttc tggttcacct cttcctcaga
     3541 caggccggtg cgctcacaga tggaatggag cagatgatcg tgcatctctg ccatcttctg
     3601 gtccatgtcg aatttttcgt ggcagcaatc tctgcgccat gccttcggaa atacggcttc
     3661 cccccctcat cagactgtgt gtttggttcc agctctttct tccgggattc ttcgctctcg
     3721 gaaatcggtc tgtacagtga acacacataa atattcttcc tatgactggt atcactcatg
     3781 gcttgtctcc tttatttgtt ttgtcgagtc ctacctcaaa ggaggtctgg ccagcatata
     3841 ggtgttcatc tgactgtccc tttcaaaatt agtgtcctca tctatgattt cgggcgtttt
     3901 ataactcctc cggacaaaat ttttgaaaaa atttgtcgag ggcttaatac acctctttca
     3961 taactgcaaa cgcacgcttc aaggtcttgg tgatttctgc atgcgacatg cgcagatccc
     4021 tcgcaatctg ctgctggttc ttgttcagga agtaataatg ctcgatgacc tttctctgat
     4081 taaaggttag cttctggatc tcttcttgca gacgcttacg ctccatcttc ttaaaatcag
     4141 tcggagcaat cttctccaca atgtcaggag tataggacat catgcagtcc tgtgtgtagc
     4201 tgtcctcgat ggtctgctct atgcctccga ggtatcttgc atagttggat tccagacggt
     4261 tctctgcatc atggtcatgt agatggtcca ctgatagtcc atatactcct caaccaggat
     4321 gaagctattc ttatcggcga accgatacaa cattccattc atgcggtcag ctgtgaagat
     4381 ggagtacata tcattgcgaa tatagaggat aaagtcgttg cgatacacgg tcaacagaga
     4441 atcgtccatc tagaactgag ccacactcac ttggcaccat attatcactc agctaacgga
     4501 agccgggctg ctttacgcct ttggtgctgt ccttgatttc cttccatata ctgcttgccg
     4561 agattgatga ttcttctgac atcgatcaaa gtaacaggta atttagaatc ttccacaaac
     4621 ctgattatct cctctactac tttcggctca atatcttcta atgcgatcac gattttatat
     4681 acctgctact tcacaataac atcctgaaca tcagatattc gtccaaaata ctttaatttc
     4741 gccaatccat ccccataatt agcaaatcct ggttcttcaa gtagaatatc ctctttcttt
     4801 cgatctttca catatccaat aatatttgca tctgcgtctg gatctgataa catggccaaa
     4861 ccatattctt tagccaggtt tccattttcc acaaccaaaa ctcgagtatg ggacttgctg
     4921 cctatctttt tcagtaaaaa ctgaaataca gcattcttga cgagcaacaa aacgcacgat
     4981 atgatccaaa ataaaagcaa tggccatttg gaaaagtatt tcacattagc gatatataag
     5041 tataagaaag caaggaagaa caaacttcca ataccggtta acgatactat tttaaaaatc
     5101 cgttttgata ctttctgaaa taggtcttat tcgttgcacg aaaaatcctc aaaaaatctg
     5161 caatcaaaat actatatagc gcagccatca tcaaatacgg aactgacaac atattgatcc
     5221 cgggagcagc ttctaaaacg tggtaacaaa taaacatggc caatagatag gctacaaaaa
     5281 tagatgctgc atctccaata cagtcaaaaa ctgtgttcca aaatcgttcc ttgataattt
     5341 cattgattct ccccttataa taatatcgaa tgctccaaca tcggtcagaa tcatctcttc
     5401 tgtctatcat tcgccacgta gaagttgttg gaaaagagtg acaaccgttc aagacgaacg
     5461 gctatattgt attaattatt ctgtgtatca actaaccact caacaaattc acgcactgca
     5521 ccattaccac catcatgttc agcgataaaa tctgaaattt ctagaacctt tttaactgca
     5581 tcgtgcggac aggccacaat accacctgca cttttaacag gatgcataca agataaatcg
     5641 tttatatcgt ctccaatata tgcaacagta cttagatcac tagtaatctc aagcagtttc
     5701 tctattttat tgtctattcc ctgataaaga atgttgatac caagttcctt acatctattt
     5761 tctaatattt tggaacgacg accagtgata ataataggct caatattatg ggctcttaca
     5821 atatcgtgaa tagcgcaacc atctttaata ttgaacgcct tcataatttc cccacactca
     5881 cccatatata tctttccgtc tgttagagta ccatcaacat ccataaccag aaatttaatt
     5941 tggctcatac actttccccc cctttcaata attgatagaa atcttaaata cagctttctt
     6001 tactttagct gattcattca ctcttatgca aggcatatga gcatcctcag gagttagtag
     6061 cagcagcttc cctccttcta agagtacaca attgccaact attgaattac tgtaaaacac
     6121 aatatccttg tcagctgaat aatctacttc aatctgtaaa ttagatatag attctactac
     6181 aatatactcg catcctaaga gaatacattg aatgtctaaa tatttcaagt gagattcgta
     6241 cattctttct tcaaatctat atatatcata ggactcaaca ttcagataaa ttccattgga
     6301 aagatcatat cgaccttctt ctaattccaa caaggaattt tttataagaa aatatagact
     6361 tctggaaatt caatttccat ttttttagac ttaacttttt catttatctc ctttatgcaa
     6421 caataccagc ttttacaatt tgctgccatg taatggtacc aacaagcata ccttcatcat
     6481 cgattatagg aagaatgtta atactattag cctttaaaaa tctcaatgca tcgacagcta
     6541 atttatcttt agtaaatact ttgggattag caatcatcac atcatcaatt tctacagaat
     6601 agatatctac ttttctctca ataattcgtc ttaaatctcc atcagtaata atgcctttga
     6661 gagttccatc ctgatcaatg actgaaacta tacccaaccc tttattcgac ataacagaaa
     6721 tagcatccat taacaatgtt cccgaattga ccattggaat ctcattgccg tgggacatta
     6781 gatcagacac tttaagtatt aattttttgc ccaatgaacc tgcgggatga aactttccaa
     6841 aatcagaatc tttaaatcca taaataccgc tagcaacaac agctaatgca tcgccgtaac
     6901 acaacacact tgtagcacta gaagttggag ccaatcccaa atagcaagct tcctcaaact
     6961 ccggtaatat ctggacaata tcagatgcgg tagcaagcgt agattttgaa ttgcctgtta
     7021 ttccaattaa agtagcccca atcattttaa tattaggaag aattttaaca atttcatcgg
     7081 attcgccgct atagctgata gcaattacaa tatccttttc tgaaaccata cctaaatctc
     7141 catgcatcgc ttcagcagga tgaagtctga atgctggtgt tcctaagctt gcaaatgtcg
     7201 ctgccagctt agcagcgata tgacctggct ttcccatacc agtgataatg acttttcctt
     7261 cgcaattaac aattgcatca agaatacgaa cgaaagtctt cccaagacaa tctctcgttc
     7321 tttttaaggc ctcaatttca atatcaaaaa ctcttttggc ctctattatc ttatccattt
     7381 tatttacctc ttcagttttt aatagcagct ggaggtcttt cacatgttgt aacacagtac
     7441 aaaatgtgaa agacttacca tacatttcta tgcaatgcaa gaaaaattat ctaacggcat
     7501 tgtatacttt tagtacacgc tctaataacc cctcaaattc atccaagaga accatattcg
     7561 gaccatcaga caaggcattg tcaggatcag gatgtacttc aaagaacaaa gcatctgcgc
     7621 ctgttgcaac agccgccttc gcaagaggct caacgttatt tctattacct cctgtaacat
     7681 ctccttttcc accaggcttc tgaacactat gcgtagcatc cataacaaca gggtaaccaa
     7741 acttcttcat ctcaacaata gatgtcatgt caacaaccaa tgtattatat ccaaaagaag
     7801 tgccacgttc gcaaagcata atgttactat tacctgcctc cacaagttta ttaataacat
     7861 ttttcatatc ccacggtgcg agaaactgtg ctttttttac gttaattatt ttgcctgttt
     7921 ttgctgctgc cactagtaaa tcagtctgtc tacacaggaa tgcaggaatc tgaagtatat
     7981 cgcatacctc tgcagccttt tctgcttgcc aaggttcatg gatatctgta caaattttca
     8041 atccataagt ttccttaact ttcttaagga ttttcaaacc ctcttcaata ccaggacctc
     8101 tgtaagaggt aatactagtt ctgttagctt tgtcgaaaga agccttaaaa taataatcaa
     8161 tatcgagctt ctccgtaatc tccttaatag tcttagctat taacattaca ttttcttcgg
     8221 actcaataac gcatggtcct gcaattaaca taagtctatt catcttatag accttccttt
     8281 cttaattcat cttctacctg ctgtatctgc tccggcgtat ccacccccac ggtttgatat
     8341 ttagtttcct ttactcgaat cttaaatcca ctctccaaaa ctcgaagttg ttcgagagat
     8401 tcagatactt caagaggcgt tttgggcatt gtactgtatt tcttcaaaaa ccatgtttta
     8461 tacccataaa caccaatatg tttaaaatgc agagaatttt ttccatctct ttcgtatgga
     8521 acacaaaaac gtgaaaaata tatcgcataa tcattgacgt cattgattac tttaacaaca
     8581 tttggattgt caagttcctc ctgaacttca atatgttttt tcaaggttcc catataaact
     8641 tcatcgttat caaagcaaga atacaaatct gctatcatct ctgccttgat aaggggctcg
     8701 tctccttgga tattaagaac taagtcttca tcccggagtt gaaggatctc tgcgcactca
     8761 gcaagacgat ctgtgccgca agtatgttta tcagaagtca tcaaagccat tccaccaaag
     8821 ctttcaacac agtcaaaaat tctcttatcg tctgttgcaa catatactgc atctagcata
     8881 gctgccttag aaacattttc ataaacccac tgaatcatgg gtttactaga aatcatagca
     8941 agcggcttcc ctggaaacct acttgatcca tatcgagatg gaataatagc tattgtcttc
     9001 atcggttctt tctcctttta agataatcca ttaacaactt atctttcata catattagta
     9061 aaatactata tagtaaaata cacaaaatta ctgtaatgaa cgttgatatt actgaatttg
     9121 ggagcttact actgacatat cctccgacgc ctcccataat cagtgcacct atcaaatagt
     9181 tttttacatt cgtaataatt gatgccattt ttaattctat acagtttctt acttcataaa
     9241 actgaattgc agtaactgta agttcagcaa atattgtacc tattgcagcg cctatagctt
     9301 ccaaatgtgg tattaaagcc aaattgacaa cgagattaat cacagcgccc gtcataatag
     9361 agaaggtata caccctttgt cttttcatgg gaatcaaaac agcttgacca accacactag
     9421 caaatccaat tatcaaaatc aatggtgata gtaactgaaa taaggaaact gacttagcgt
     9481 atccgtctcc aagaaaccac ggtataaaag tatttgcgat tataatcgca ccaactacca
     9541 ttggggcgcc tatgcaaaac acaaatgata ctgcattcaa tacttctttt ctaattctat
     9601 caatattgtt ttctttaacc gctactgcga tatgtggcaa cataactgca ccaagagatg
     9661 taatgactgt catcaacagc ttcactattt tttcaggctg cgagtatagc ccaacttgga
     9721 catccgtaga aaatattcct aatataatct tattaacata cgtatagata tatactgccg
     9781 ctgttggaag aaataatcct aatattggtc gaatatgggg tgttatcgat atatcatgaa
     9841 tacttattct tctaacatat tttggaatat gacaacataa taatgcattc cccaacagct
     9901 cagttccagc caatataact gagtacaata ctacatcatt ttcatttttc accaataaga
     9961 atatcaaaac aagaccaatt aacttaacca ccatacttct gattgcagtt tttttgaaat
    10021 cttccatccc ttggaaaaac caactgacat caaaaagatt tgcaactaaa taaacagaca
    10081 tacttattag taaaacagtg tatgaatgaa gagtagccga aataagaata tatacaggaa
    10141 aaacgataaa cgaagaaata atacggataa caaagatgct ccaaaacact ccacttcttc
    10201 tgatcttatc attctgaaca taagcaatct cacgttgtcc atacatgttc gtacctaatt
    10261 gagcaattat taccgctacc gatacaatgg cgtacgaata attaaactcc ccaactccct
    10321 ccgctttcaa tactctagat acgtatggag tcgtaataat aggcagtatc ataaccaaaa
    10381 tttggtaaca aagattatag gcataatttt tgatcaacct attcatatta ctcctccttt
    10441 atcttaaata taatttcatc ctgccttttc gtaaattctc gtactaaaag gcaagcaacc
    10501 acaacagaaa atatatttgt tatagaaaac gcgaaacaca tctggcaata gtagaaactc
    10561 atcactggta tgtaaaagaa gtatgccgta attagtatat agtatttcct tcttcttctg
    10621 tttttttcct tattaatgcg aatatgaata ttccaagaaa aaatccaaaa aacaataaaa
    10681 gtaaacacca gcatacctaa ccaaccaaaa tcagagtaag gtcgttgaaa ccacgaaaat
    10741 tcattccccg caaagttact atctccacca acatggatat atgcgttttt aaattctggt
    10801 tctataccaa acatctctac caatgaggta agactttttg ccccccaatt caattctgga
    10861 gaatatctct ggagatattt atccagcata tatactgggc ttccgatata ataagtaata
    10921 tagtctgaaa actctcttgt tgccgcagtc gagttaactt ttgtaatacc tcttaatgca
    10981 taaaatacag ccaaaatcgc tactgcaacg aataaagttt ttcgcacaac ttgcataaca
    11041 ggaactcgtc cacgaggcga tacatctgac ttcattacta tgtatatcaa aaatactata
    11101 gcaaaaagct taactaaaaa cacacggtta ccggatataa acatacataa aatatacatt
    11161 aatgttggaa acaagtattt tatatttgat aaaacacgtt
//
LOCUS       DAZKAO010000090        11079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000090 DAZKAO010000000
VERSION     DAZKAO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.090"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1124
                     /gene="pplA"
                     /locus_tag="ACG8H8_002034"
     CDS             189..1124
                     /gene="pplA"
                     /locus_tag="ACG8H8_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP8987090.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1214..2317
                     /locus_tag="ACG8H8_002035"
     CDS             1214..2317
                     /locus_tag="ACG8H8_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP8987091.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2330..3283
                     /gene="menA"
                     /locus_tag="ACG8H8_002036"
     CDS             2330..3283
                     /gene="menA"
                     /locus_tag="ACG8H8_002036"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP8987092.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4097..5443)
                     /gene="gor"
                     /locus_tag="ACG8H8_002037"
     CDS             complement(4097..5443)
                     /gene="gor"
                     /locus_tag="ACG8H8_002037"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP8987093.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5577..6830)
                     /locus_tag="ACG8H8_002038"
     CDS             complement(5577..6830)
                     /locus_tag="ACG8H8_002038"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP8987094.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6836..7759)
                     /locus_tag="ACG8H8_002039"
     CDS             complement(6836..7759)
                     /locus_tag="ACG8H8_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP8987095.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7980..10472)
                     /locus_tag="ACG8H8_002040"
     CDS             complement(7980..10472)
                     /locus_tag="ACG8H8_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP8987096.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10479..10946)
                     /locus_tag="ACG8H8_002041"
     CDS             complement(10479..10946)
                     /locus_tag="ACG8H8_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP8987097.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3265 a         2263 c         1941 g         3610 t
ORIGIN      
        1 actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa
       61 agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa
      121 aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg
      181 gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc
      241 tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat
      301 ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg
      361 gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc
      421 gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg
      481 tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa
      541 aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac
      601 aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa
      661 actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg
      721 acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg
      781 gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg
      841 attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga
      901 aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca
      961 aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac
     1021 acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg
     1081 acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta
     1141 ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga
     1201 agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt
     1261 tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc
     1321 taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg
     1381 tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa
     1441 agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga
     1501 gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc
     1561 ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca
     1621 attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc
     1681 attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct
     1741 aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga
     1801 agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa
     1861 tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca
     1921 agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt
     1981 agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt
     2041 gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac
     2101 agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa
     2161 aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa
     2221 agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc
     2281 ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa
     2341 aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt
     2401 aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt
     2461 ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta
     2521 tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct
     2581 atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg
     2641 cgttattcta acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt
     2701 tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat
     2761 tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt
     2821 cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg ctttggtagg
     2881 aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc
     2941 caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta
     3001 tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta
     3061 tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt
     3121 ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt
     3181 accccagcct atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta
     3241 tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt
     3301 gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt
     3361 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt atcaggaggt
     3421 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     3481 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg
     3541 ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat
     3601 attttgccgt tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa
     3661 aaagtgacta actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg
     3721 taccagcgaa cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat
     3781 cctcgtttgc ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt
     3841 cgatacattt ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac
     3901 caaatggatt catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta
     3961 cttatttcgt tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac
     4021 cagaaaatca gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg
     4081 aactgttaat attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg
     4141 ctactgtatt gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt
     4201 gcagcatctc atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc
     4261 cgacacagat caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg
     4321 gggtaaagcg tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt
     4381 cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt
     4441 tatagtcaag ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag
     4501 ctgcgattgc aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat
     4561 gttcatccgt tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca
     4621 catttgtatt ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta
     4681 cacaatcacc agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt
     4741 tagtaatttc tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt
     4801 ggatcaaatt gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg
     4861 cccacatgac ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc
     4921 cagcacctaa aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat
     4981 caattgcgta ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca
     5041 agattttagg agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag
     5101 tagcgtaacc ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt
     5161 gtagaaaatc aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta
     5221 aatctgcttg gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg
     5281 cttgccacat aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat
     5341 tcgcttcaat cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac
     5401 cgccagagcc gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg
     5461 aagagatcaa tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac
     5521 ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat
     5581 ttacgttttc ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc
     5641 atcattggat ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg
     5701 gtttgactga aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac
     5761 aagtttctct gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg
     5821 acaaatttat atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt
     5881 gtattgtagt aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc
     5941 cattcttgct tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta
     6001 tagacatttg acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag
     6061 ctgtctacgc cgttttcaat caattctgga atatgttcga tcattgacat atccactgca
     6121 ctcatcgaga attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca
     6181 aaaggcatat cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt
     6241 gcatcccgca tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg
     6301 gcaccatgga taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc
     6361 tccattgaaa cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc
     6421 aatgtctcaa aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct
     6481 tctgtcgcac aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca
     6541 tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata
     6601 ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt
     6661 tcttcataag taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat
     6721 actgcatctg ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct
     6781 aggatttctg gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt
     6841 tgatagcaga aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca
     6901 atttactgat ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa
     6961 tttgtttttt tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa
     7021 ataatccgtc caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg
     7081 agagattcag atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat
     7141 aatgtgtctc ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt
     7201 cgtcttgttt cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc
     7261 atgtagcacc atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca
     7321 tctcttcaaa aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga
     7381 aattgatttg acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca
     7441 attcaggatt ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta
     7501 cgtttatttc ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa
     7561 tgatcccatt tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt
     7621 gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga
     7681 aatatagtgt atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg
     7741 tggttatgag ttcgatcata attttctcct cctacaactt cataatagta gcatagctct
     7801 aaaaactttc caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa
     7861 atcaaaataa agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt
     7921 gtcacaacct cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac
     7981 tatagggaac ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt
     8041 gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc
     8101 aatcgatctt caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga
     8161 tcaaatccag ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct
     8221 tgttctttca atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca
     8281 tctttactca atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg
     8341 aaagctttct ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca
     8401 tcttggacat tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa
     8461 gtcataatca tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga
     8521 ccatcatcca atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca
     8581 tctaagagaa tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca
     8641 tcatacccta cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata
     8701 aattctgaca tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt
     8761 gctttggcca attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt
     8821 ggacgatcag gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg
     8881 acagcctcat cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt
     8941 tcgctttctt ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta
     9001 gcaacatctt cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca
     9061 gaaacttttt gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt
     9121 tcaaagtctt gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt
     9181 ttgatcacag ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat
     9241 tcatcgatca aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa
     9301 agattgactg ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat
     9361 cttgtacgta atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga
     9421 atgcgagcaa aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct
     9481 agggtcgttg ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc
     9541 gaagcatcaa ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg
     9601 aataagatta cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca
     9661 aattcaccgc gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc
     9721 ttttcttgca tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca
     9781 atggctgttt tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg
     9841 ctaagaatct ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg
     9901 ttttcttttg ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc
     9961 ggtgtttgcc gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca
    10021 gaatcaccag ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata
    10081 cctaagttga tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa
    10141 atatgttctg ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca
    10201 aaaacttgtt tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata
    10261 ccaccataac caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt
    10321 tcacgtaata cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt
    10381 tctgaactaa ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact
    10441 gcttgggctc tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt
    10501 aaacgttcca gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa
    10561 ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt
    10621 tggtcttcat aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca
    10681 aataactgat ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt
    10741 cgtatataac caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa
    10801 cgggtattaa tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca
    10861 atccttcgaa tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt
    10921 aaatccgagg tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa
    10981 ttataacgaa tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag
    11041 aatcaacaaa aaaaaatacg gaatatacta ttccgtatc
//
LOCUS       DAZKAO010000091        10791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000091 DAZKAO010000000
VERSION     DAZKAO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.091"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..979)
                     /gene="nrdF"
                     /locus_tag="ACG8H8_002042"
     CDS             complement(14..979)
                     /gene="nrdF"
                     /locus_tag="ACG8H8_002042"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP8987098.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1029..3188)
                     /gene="nrdE"
                     /locus_tag="ACG8H8_002043"
     CDS             complement(1029..3188)
                     /gene="nrdE"
                     /locus_tag="ACG8H8_002043"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP8987099.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3347..3571)
                     /gene="nrdH"
                     /locus_tag="ACG8H8_002044"
     CDS             complement(3347..3571)
                     /gene="nrdH"
                     /locus_tag="ACG8H8_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP8987100.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3972..4445
                     /locus_tag="ACG8H8_002045"
     CDS             3972..4445
                     /locus_tag="ACG8H8_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP8987101.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4442..6574
                     /gene="feoB"
                     /locus_tag="ACG8H8_002046"
     CDS             4442..6574
                     /gene="feoB"
                     /locus_tag="ACG8H8_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP8987102.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6576..6710
                     /locus_tag="ACG8H8_002047"
     CDS             6576..6710
                     /locus_tag="ACG8H8_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP8987103.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6804..7448
                     /locus_tag="ACG8H8_002048"
     CDS             6804..7448
                     /locus_tag="ACG8H8_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8987104.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7635..8828
                     /locus_tag="ACG8H8_002049"
     CDS             7635..8828
                     /locus_tag="ACG8H8_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8987105.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8821..10548
                     /locus_tag="ACG8H8_002050"
     CDS             8821..10548
                     /locus_tag="ACG8H8_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP8987106.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         3371 a         1933 c         2254 g         3233 t
ORIGIN      
        1 ttaaaataaa tccttattcc aatccaatca aataatcgtc gtctttcatc gcttctacat
       61 gacctaacaa atagccgttt ccgacttgtg agaagaagtc atggttgctt gtgcctgttg
      121 aaatcccgtt cattacgatt gggttgacat cattggcagt atctggaaag agtggatcca
      181 tccctaggtt catcaatgct ttgttggcat tatagcgtag gaatgtcttg acttcttctg
      241 tccagccgat tggatcgtat aattcttctg tataacgttc ttcgttttcg taaagctcat
      301 aaagaagatt gtacatccag tcttttaacg tttcttgttc tgcttcaggt aactcgttga
      361 atcctaattg gaatttgtaa ccgatgtagg ttccatgtac agattcatct cggatgatta
      421 gtttgatgat ctctgctacg ttagccaatt tgttgtttcc taaataataa agaggagtat
      481 aaaaacctga atagaacaag aatgtttcta aaaagacgct ggcaattttc ttttctagcg
      541 gtgtaccgtg tttgtagatt tcattgatac gctcagcttt tttttgcaga taaggattcg
      601 tatttgtcca gtcaaaaatt tcttcgattt cttttttcgt attcagtgta ctaaaaatag
      661 aagaataact tttggcatga acggattcca tgaattgaat attgttcaaa acggcttctt
      721 catggggagt gcggacatct ttacgcaact gatccatgcc actttcagat tggacagtat
      781 ctagcagtgt caaaccgcca aatacatgtc ctactgtatc tttttccaat tgagacaatg
      841 tacgccaatc atctaggtca tttgaaagtg ggatacgtgt atctagccaa aattgctctg
      901 tcagcttctc ccaagtggat ttatcaatga tatcttcaat ttcattccag tttattgctt
      961 cataatatgt ttctgacatg agaatgctcc tttaattaaa atatgaataa gcgaatctat
     1021 ctatgatatt agatcacgca gctttcacat tgattgcttc cgatttcttc tgcatcatca
     1081 gtaaaggtac gtacgtaata aatggatttg attcctttgt ggaaagcgta atgtcttaaa
     1141 atattcaaat cacgtgtcgt ttgcttcgtt gttgtcttcc attcataaat gccttctggg
     1201 atttcagaac gcatgaataa agtcaaactc atgccttgat cgacgtgttg ttgtgccgtt
     1261 gcataaacat cgattacttt acgcatgtcc atatcataag cagaagtgta atacggaatc
     1321 gtctcattgg atagataagg cgctgggtaa taaattttac cgattttctt ctcttggcgt
     1381 tcttcgatca tgcgagtgat aggatgaata cttgcactcg tatcgttgat atatgaaatt
     1441 gagccatttg gtgctactgc caagcgattt tgatgataca gtccatcttt cattactgct
     1501 tgtttcaaag cattccaatc ttcagcagtt gggataaaaa tgccttcaaa caatgctttg
     1561 actttttctg attttggctg gaattctcca gttacgtatt tttcgaaata agtgccgtca
     1621 gcataagctg atttttcaaa attatggaaa acttgttttc tttcacgagc gatattgttg
     1681 ctttcaacaa gtgtccaata attcaacagc atgaagtaaa tatttgtaaa gtctaatgaa
     1741 tcctctgagc catattccat ttgttctttt gcaagataag tgtgtaagcc catggcacct
     1801 aatccaatcg tatgattcag cttatttccg ttttggatcg gtggtacgac atcgatatca
     1861 gatgcatctg taataaatgt caatgcacga gtcatcgtac gcacagattg accaaagtta
     1921 gggctttgca tcaaattcac gatatttgtc gagcctaagt tacatgagat atctgttcct
     1981 aatacttcat attcctgttt gccgttaatc acagaaggcg tttgaacttg taaaatctct
     2041 gaacataaat tactcatgat gatcttgcca tcgattggat tctcgcggtt cgctgtatca
     2101 atattgatta cgtaaggata gccagattct tgttgtaatt ttgagatttc attttcaaga
     2161 tcacgcgctt tgattttttg tttgcgaatg tttggatttg caactagatg atcatactca
     2221 gctgtaatat ttacataaga gaatgggaca ccatattctt tttcgacact atatggacta
     2281 aataaataca tatcttcgtt tttacgtgtc aattcataaa atttatcagg tacgatcacg
     2341 ccaagagaaa gagtttttac gcgaatcttt tcatctgcat tttctttttt tgctgaaagg
     2401 aaagcaataa tgtctggatg gaaaacgttc aaataaacca cgccagctcc ttgacgctga
     2461 cctaattgat tagaatagct gaaactatct tcgaataatt tcatgactgg aacgacacca
     2521 cttgcagcac cctcatatcc tttgatagga gcgccggctt cacgaagatt tgataaagta
     2581 atccctacgc cgccaccaat acgggacaat tgcaaagcag agttgattga gcgcccgata
     2641 gagttcatat cgtcagtgac ttgagtcaag aaacaagaca ccaattcgcc ccgacgttta
     2701 cgtccagcat tcaagaaaga aggtgtagct ggctgatatc gttgatggat catttcgtct
     2761 gcaagagcaa gtgctaattc ttcatccccg ttcgcaaagt aaagcgcatt gaaggctact
     2821 cgatcttcat aagtttccag ataagcggaa ccgtcgttgg ttttcaacgc atattgagaa
     2881 taaaatttgt aagcagccat aaaagatttg aaagaaaaat gttgctcgtc caaaaattga
     2941 tatagctttt caataaatga tggtgaatac aattgaatga attctgcttc tatatattct
     3001 tgatcgatca agtaattgat tttttccata atagatggaa aagtcatcgt atttggtttg
     3061 acattttcct caaaaaaggc aactaaagct tctttatcct tattcaaagg gatttgacca
     3121 ttgactggcc gattgatttc attattcagc ttgaaataac tgacatcttg taaattttta
     3181 agactcatac gtacgcccaa ccttcatttc gtatttgatg atgcttgatt gcaaatcatt
     3241 gcattgtttt taagctttaa aaaaggattt ggaagtttgc gacaaaaact ggtgctgtct
     3301 caagcccttc aaatccatgt atcacgtttt gattctttta gtcatgttag ctggcaagtt
     3361 tacgcaactg atcaggacgg aatccgataa ctgtttctgc atcagaagta atgacaggaa
     3421 cactttggaa tccttgtgct ttcaaccagt ctactgcttc aggctgttca tcgatattga
     3481 cttcttcaaa agcaatttga ttttctgtta aaaaccgttt gaccatctta cattgcatac
     3541 aattattttt agaaaagatt tttacgttca tggtgagtgc ctcctctaac acattcttat
     3601 tatctataat agatagtata aaactcttag taaaaaagtc aaacaacaaa cactacatat
     3661 tgtgttgtgg aaactgtgga atactaagtt ttgtgttttt gaaaaaagag ctgaaaacaa
     3721 gtatcgtgtg ggaaaatagc aaatttaata gagaaaaaat tgtggaaaac tatgaagaaa
     3781 tatttttttc tcatttttat ctatcaatac taaagattcg ctcataatat cacaagtata
     3841 attgacattc tcaattaccg agagtattct atgtgagaat gattctcatt tatttgcgta
     3901 acacgtaaga aaattagaac atataatgaa tgattttaat acatgattta tttcacaaga
     3961 gaggaatcaa tatgcagacc ttagcgcagg caaagcagaa tttcgtttat caagtcgaac
     4021 gaatcgaaac agaagaaaaa acaaaacgcc atttgcaaaa tatcgggatc gttgccggaa
     4081 gtaaaatcgt cgtcgtcaat ttatcaggca acaatgccat tttactggta aaaagtcatc
     4141 gagtggcagt aagtcgggaa atactaggtc aaatctttgt caataaaaag actgcttcct
     4201 ctgaaagctg gacttctctt gatcaattaa aagcaggaga gactgggatc gtcgtagcaa
     4261 tccacggaca aggcgcagtg aagcgacggt taatggatat gggattgacg cgaaatgtaa
     4321 aaatagttat ccgaaaacta gcccctctgg gagatccaat cgaactgaca gtgcgagggt
     4381 atgaactgac gttacgaaag tcagaggccg aactaatctt gatacaaagg gaggaaaaag
     4441 aatgaatatc gcgttagtcg gaaatccaaa cagcgggaaa actagcttgt tcaatctctt
     4501 gactggttcg aatcaatatg tagggaattg gccgggcgta acagtagaaa aaaaagaagg
     4561 atcgttgaaa aaaaatccga acattcatat tcaggaccta cccggcatct attctttgtc
     4621 tccttataca ccagaagaag tgattgcaag agattatttg gttcaggaac agcctgaact
     4681 gatcatcaac attattgatg ctaccaattt agaacgaaat ctttatttaa ctacgcagtt
     4741 attggattta gacattccta tgatcattgg tctgaatatg atggatgtca tcaaaaaaga
     4801 aggaaaaaaa atcaatctag aaaagctttc ctatggtctt ggtgtaccag ttttaccaat
     4861 cagcgtattg aaaaagcaag gaatcgaaaa actaatcgaa aagattcagg agtctgcaaa
     4921 aaagcggcct tctattcctg ttccctgtac atatgatcca cgtttagaag cagctttaca
     4981 tgaaattgaa gatgtactag gagaacaaag cggcaagaaa cgctggtatg caatgaaata
     5041 ttttgaacga gatcagaagg taagagagga ttacgagata gcagtttctc agcaaaaaga
     5101 gatcgagcaa ctgatccagt taacagagaa actattggac gacgatagtg agacgattct
     5161 ggtcaatgaa cgttatgaat ttattaccca actatgtaca ctctgtgttg tgtcaaatga
     5221 ttctttccaa ttatcgatga gtgacaaaat cgatcagatt gcaacgaatc gttggttagc
     5281 cttgccgatc ttcgcatttg tgatgtggct gatctattat ctggctattc aaacagtagg
     5341 aacaatgggg acagattgga tcaatgacac attgtttggt acatggttac cagaacatgt
     5401 tggcagattg ttagctgaat ggcaagtagc gggatggatg caggagctga tcttgaacgg
     5461 catcatcgca ggcgtgggag cagtgttagg atttctaccg caattgatta tattgttttt
     5521 atgcttgagc tttctggaag actgcggata tatggcgagg atcgcttttg tcatggatcg
     5581 acttttcagg aaattcggtc tttcaggaaa atccttcatt cctatgctga ttgctacagg
     5641 atgtggagtg ccaggcgtca tggccagtcg cacgattgaa aacgagcaag atcgtcgttt
     5701 gacgatcatg gtcacaacct tcatgccttg ttctgcaaag ttgccgatta ttgccttagt
     5761 agcaggggcc ttttttcctc atcaatcttg ggtagctcct tctgcctatt ttcttggtat
     5821 gactgcaatt attttctctg gaatcagctt gaaaaaaaca cgtttgtttg ctggagatac
     5881 agctcctttt atcatggaat tgcccgcata tcattttcct caatggtcag cggtcctaag
     5941 acaaacctat gaccgcagca agtcttttgt taaaaaagca ggaacgatta tctttgtttc
     6001 tagtatcgtg atctggttca gctcttctta taatttttat gctcaacctg tttctgaaga
     6061 ccaaagtatc ttagcctttt tcgggcgaat cctggcagtt gtttttcagc cgcttggttg
     6121 gggaaactgg caaggaacag tcgcagcgat tacaggattg gtcgctaaag aaaatattat
     6181 tggcacattt ggtattttgt ttggtcatgc ggaagtatcc gaaaatggca gagaaatctg
     6241 gcaagtgcta caacacacat atactcccgc agcagcttat tcattgttag ccttcaattt
     6301 actttgtgcg ccttgttttg cggcaattgg ggctatccat cgggaaatga atgccaagaa
     6361 atggacttgg atcgccatag gctatcaatg cggtttagct tatttagtta gttttgtgat
     6421 ttatcagttg ggacacttat tggagggtgg acaggtagcg ttagggacgt atcttgctat
     6481 tttactgatg ataggactcg tttatgtttt gttccgtcag ccaaaatcca agaaccagtt
     6541 ttatactata ttgtcattag aaggagagga ataggatggc gacattctta ttaagtgtgt
     6601 taatctttgg aagtgcaggc gcaattgtct atactcgttt aaaatccggt aagaattgtg
     6661 atgactgtca aacagcttgc ccagtaaaaa aggaacagat caagcgatga tcagtcgttg
     6721 caagaggcta ttctcccctt ttttgcttat tttgctagag catagtaaga tgagggaaag
     6781 taaaaaaaga aagaggaaag aaaatgtctg gaaagaaaaa aatcgtccgc ccgcgctacc
     6841 agcagattgc tgttgattta gccgaacgta tcgttgaaaa ccgatataaa gtgggtgaga
     6901 agatccatgc ccgctcaaca cttgccagta cgtacaatgc ttctcctgag actacccgca
     6961 aggctatcaa tgtgctggta gatcttggaa tcatggaagt acggcatgga agtggggcat
     7021 ttgttgcttc gaaggaaaaa gcaaaagatt tcctattgca atatcaagat gtacaatcca
     7081 tccaagatac aaaagcagaa atcatggcaa gtgtggagaa gcaacaagat gagctgaatc
     7141 atttttctca attattggat cagctggtca atcaaacgaa gcgaatacat aagatgaatc
     7201 ctttacttcc gtttgaatta tctttatcag aaaaagcaat gcatctggac cgttcaatta
     7261 gcgagttgaa tatctggcag gcgactggcg ccacagttat cgcaatttct cataatgaag
     7321 aactgcttgt ttcacctggc ccctatgcta aatttacggc aggggacatc gttcatttta
     7381 ttggaaatga atatacacta cagcgtatga caaacttttt ttacccggag catcagcttg
     7441 cagaatgatt gtgtgaaata ttaaaaaaat attaacttgg ctaaaccttt tatttacaaa
     7501 gatttagcca agtttttttc agtttggaca agcaaatttc tttcattttt gacaaagtga
     7561 ctacatgatg ttaacatagt gtggtcactt ttttcagaaa gaaaaatgaa tttacataaa
     7621 aaaggagaat ttgtttggta aaagtagaag taaaacattt aacaaagata tttggtaaaa
     7681 agacacaagc cgctttagat atgatgaatg atcaccagcc gaaaacggag attttaaaga
     7741 aaaccggtgc aacggttggt gtgtatgacg tgaattttga cgtaaaagaa ggagaaatct
     7801 tcgtgatcat ggggctttcc gggagtggga aatccacatt gatccgtcta ttgaatcgtc
     7861 tgattgagcc aacttcgggc agtatctata ttgatggaga agatgtatcg aagttaagca
     7921 aagaagaatt gcgagaagtc cgtcgtcata aaatcaatat ggttttccaa aacttcggtc
     7981 tgtttccaca tcgaacgatc ttggaaaata cagaatacgg gttagaagtc cgtggtgtac
     8041 ctaaagaaga acgacaagaa aaagcagaaa aagcattaga aaattctagt ttgctttcat
     8101 ttaaagacca atatcctagt cagttatctg gtgggatgca acaacgggtt ggcctagcca
     8161 gagctttagc aaatgatcct gagatcttgc tgatggacga agcgttctcg gctcttgatc
     8221 cgctgatccg gcgagaaatg caagacgaat tactggacct gcaagccaat gttcaaaaaa
     8281 cgattatttt cattacccat gatttgaatg aagctttacg gattggggac agaatcgcct
     8341 tgatgaaaga cggcgaaatc atgcagatcg gaacaggtga agaaatattg accaatccag
     8401 caaatgatta tgtacgtgaa tttgtggaag aagtcgatcg ttccaaagtg cttacggctc
     8461 aaaacatcat ggtacctgca ttgacgacga atatcgaatc tgatggacca aacgttgcat
     8521 tgacaaggat gcgtaatgaa gaagtcagca tgctgatggc tgttgacaga aagagacacc
     8581 tgaaagggat catcactgca gatcaagcgt tagaagctag gaagcaaaaa cgtccattga
     8641 tcgacttcct agatgaaaat gtgacggtga ttgggaaaga catggtggta agtgatattt
     8701 tcaatattat ttatgactca ccgactccac ttgcagttgt agaggatgga aaattaaaag
     8761 gtgtcgtcat ccgaggtagt gtgattgaag cattagcaga aacaagcgag gtgagtgaac
     8821 atgaataatc tattgaacta tcaattacca gtggcaaact gggtagaaaa tatcacagaa
     8881 tggtttacga caactttttc tggcctattt tcatttttac aaacgattgg acaggcagtg
     8941 atgtcgggga ttacaaattt actgttagtg attccagctc ctttgtttat cttgttattg
     9001 acggtagcag ctttctttat ttctaagaaa cgtccaggac ttacgttgtt cacattaatc
     9061 ggtctatggt ttatttataa ccaaggtcta tggaatgatt tgatgaatac tgtcacactt
     9121 gttcttttat ctagtgtgat ctcgatcatc attggggtac cattagggat cttgatggca
     9181 aaaagcagta aagcccaaag tattatcaaa ccgatccttg actttatgca gacaatgcca
     9241 ggattcgttt atttgattcc agcagttgca tttttcggta tcgggatggt tccaggggta
     9301 tttgcctcag tcatctttgc tctgccacca accgttcgtt ttacgaattt agggattcga
     9361 caagttccta aagaattagt agaagcttct gattcattcg ggagcacaag tcgtcaaaaa
     9421 ctattcaaac tagaattgcc tttagctaaa agtacgatca tggcagggat caatcaaaca
     9481 acaatgctgt cactttcaat ggtcgttatt gcctctatga tcggtgcacc cggcttagga
     9541 cgcggcgtat tatcagcatt gcaaagagca caagtcggga atggatttgt caacggggtt
     9601 gcattagtta tcttagcaat catcattgac cgttttacac agcatttgaa ccagccaaac
     9661 aataaaaaag ctgcaggagc aacaacacag aaaagtaaaa aacgtcaagg actgatcatt
     9721 ggtgctgtag tagtagtgat cttaggtgcg attggtatag ggagtttttc atctgccaaa
     9781 gaaacaaaac gtatcaactt atcttatgtt gaatgggata cagaagttgc ttcaacaaat
     9841 gtagtcggtg aagtattgaa acaaatgggc tatgatgtaa cgatgacgcc attagataat
     9901 tcaatcatgt ggaaatctgt ttcaaatggc gagtcagatg ccatggtttc agcttggcta
     9961 cctaaaactc atggttcaca atatgcacaa tataaagatc aagtcgaaga cctaggtgcc
    10021 aatttgacag gagcaaaagt tggtttagca gtgccagcct acatggatgt aaattcgatc
    10081 gatgagttaa ccgatcaggc aggtaagaaa atcataggaa tcgagccagg agctggtgta
    10141 gttacggctg ctgaaaacac gatccaaaaa tatgacaact tgaaagactg gaaagtagag
    10201 acatcttctt caggagcaat gaccgttgca ctaggtcaag cgatcaaaaa acacgaacca
    10261 attgtagtta caggatggac acctcattgg atgtttgcca aatatgatct gaaatactta
    10321 gaagatcctg aaaacggaat gggaagcgaa gagcagatcc atacgatggt tcgaaaagga
    10381 ttgaaagaag accagccaga agcttacaaa gtattagaca atttccattg gtcagaaaaa
    10441 gatatggaaa aagttatgtt ggaaatcaac aatggaaaag atccacagca agcagcaaaa
    10501 gactggatca aagagaacca agaattagtc gaaagctgga aaaaataaaa agacagacac
    10561 gaaataaatg gagcttccaa aattggatca aatatccaaa tttggaagct ccatgttcgt
    10621 ctctatcttt tttgtctata aaaaaagaga atgcgtagct catcttcgaa acgttcaaag
    10681 acgccgacaa actattatga atttatgggt gcgagtcaaa gagatcgatc gtaggtatga
    10741 aagactaatg tgattaaagt ttattatgta cgaagtgcat tctcaaaaaa t
//
LOCUS       DAZKAO010000092        10651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000092 DAZKAO010000000
VERSION     DAZKAO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.092"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8H8_002051"
     CDS             33..656
                     /locus_tag="ACG8H8_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987107.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8H8_002052"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8H8_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8987108.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8H8_002053"
     CDS             2152..3033
                     /locus_tag="ACG8H8_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987109.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8H8_002054"
     CDS             3036..3806
                     /locus_tag="ACG8H8_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987110.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8H8_002055"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8H8_002055"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP8987111.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8H8_002056"
     CDS             5117..6280
                     /locus_tag="ACG8H8_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8987112.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8H8_002057"
     CDS             6397..6807
                     /locus_tag="ACG8H8_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8987113.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8H8_002058"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8H8_002058"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP8987114.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8H8_002059"
     CDS             9380..9664
                     /locus_tag="ACG8H8_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987115.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8H8_002060"
     CDS             9824..10060
                     /locus_tag="ACG8H8_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8987116.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8H8_002061"
     CDS             10057..10455
                     /locus_tag="ACG8H8_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP8987117.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2903 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt t
//
LOCUS       DAZKAO010000093        10411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000093 DAZKAO010000000
VERSION     DAZKAO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.093"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..498)
                     /locus_tag="ACG8H8_002062"
     CDS             complement(<1..498)
                     /locus_tag="ACG8H8_002062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987118.1"
                     /translation="MIYQLLIVEFILILIMYILSNRCFISPAFIAIALFFLATCCVVY
                     NIEFWNVQYSKKTLWFITTGFLAMCIPEMYILFLDYLSKKRVDNYHQNQYKIEYVQIY
                     PSRQLNLIFALLSVVFFGVLLVEILRKGDYSTLGLYAIGAAKSDETQNSVIARFGISL
                     ITGLNL"
     gene            complement(499..1644)
                     /locus_tag="ACG8H8_002063"
     CDS             complement(499..1644)
                     /locus_tag="ACG8H8_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HFP8987119.1"
                     /translation="MKKARVLAFYLPQFHPVRENDIMWGKGFTEWTNVAKAKPLFKNH
                     YQPQIPADLGFYDLRISEVRKQQAEMAKKYGVEGFCYWHYWFGNGKRILELPFNEVLQ
                     SGEPDFPFCLGWANHSWSNKTWQKSKHFTKDVVFLEQTYPGDEDYILHFKSILPALKD
                     DRYIKVDGKPLFLVFAPDSIPDSAHFISLWNKLAKENGFEGIYFVARVTSVDNKFDLS
                     SMTDVAVHNRFQRFLDMGYDAINSDMQRYAELKISGYWKRGIKSIMRKTVGNFSANLY
                     PYDKIMENFYCEEEKRECVHPQLIPRLDRTPRSGKDARVYVDSTPEKFGNAIDRALSY
                     VQDKEPEHRILFLQAWNEWGEGNYMEPDLVFGCEYLEILRKKILGEE"
     gene            complement(1641..2447)
                     /locus_tag="ACG8H8_002064"
     CDS             complement(1641..2447)
                     /locus_tag="ACG8H8_002064"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HFP8987120.1"
                     /translation="MVNIKIRKVIHRIPPILKLMRFVVDVQNKYFYDKFCKNTDNIQR
                     IEKLRNKFLGETCFIVGNGPSLTMEDLELIKDKDCFAANLIFKIFDKTQWRPKYYFIQ
                     DRYADTGNELDDIDLEYMFIGDYYWRVRGMHNPNAICIHSEQSVGGKNIKFSEDISKS
                     IVSHETVTYSMIQAAVYMGYKKIYLLGMDHSYALTYNADGTVSEDKTIRSHFFEDKNA
                     NEVIASIVGMNQAYIRAREFCESNNIKIYNATRGGKLNWFDRITLEEAVK"
     gene            complement(2506..2673)
                     /locus_tag="ACG8H8_002065"
                     /pseudo
     CDS             complement(2506..2673)
                     /locus_tag="ACG8H8_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016396235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
     gene            complement(3042..3176)
                     /locus_tag="ACG8H8_002066"
     CDS             complement(3042..3176)
                     /locus_tag="ACG8H8_002066"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFP8987121.1"
                     /translation="MKEKVIEYTTGVYDMFHIGHLNVMRNAKAQCDFLIVGVSTDEPV
                     "
     gene            complement(3517..4650)
                     /locus_tag="ACG8H8_002067"
     CDS             complement(3517..4650)
                     /locus_tag="ACG8H8_002067"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8987122.1"
                     /translation="MRQKIRVLHIAQAAGGVDCYIRMLLKYLDKEKFENILICSQDFC
                     ETDYANLVLSFKQIKLTREIDLNDLKAIVIIRKLIKKYNPDIVYAHSSKAGAITRVAK
                     IGLKNYCVYNPHGWAFNMRCSVKKKMLYTAIEKIAALFCDKIICISDAEKQSALKKRI
                     CCEDKLQVIFNGVDIEAYRNKAHGEVKRESLNIPQDAFVVGMVGRISPQKAPEIFIKM
                     ARLVKNKIPEAHFIIVGNGEQKALIEKYAAEHGFLESLHITGWVNNSISYIELFDVAC
                     LLSRWEGFGLALPEYMMVGKPIIATNIDAIPNLISNGKNGLLVPVDDETAACEAVFKI
                     FSDKAMAARLTSEATSVVLEKYNSKRVASEHAVLFEMLVKKRG"
     gene            complement(4787..4987)
                     /locus_tag="ACG8H8_002068"
     CDS             complement(4787..4987)
                     /locus_tag="ACG8H8_002068"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP8987123.1"
                     /translation="MFKFYKFRFVFVDDESPQIWNIFKEDMNIVGPKSGLLRKMKQYG
                     GYERQQLYVQPDLSYYWQIALN"
     gene            complement(5583..6272)
                     /locus_tag="ACG8H8_002069"
     CDS             complement(5583..6272)
                     /locus_tag="ACG8H8_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP8987124.1"
                     /translation="MESEDLLSRISKVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYAAYRIRKEEPGSPIFFSHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLVGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSGRNDVSFHAMVDLDLEYITNQSLLNDFKLIIKT
                     IWIVIKPNGAY"
     gene            complement(6326..7090)
                     /locus_tag="ACG8H8_002070"
     CDS             complement(6326..7090)
                     /locus_tag="ACG8H8_002070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP8987125.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIVMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIQRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(7118..7816)
                     /locus_tag="ACG8H8_002071"
     CDS             complement(7118..7816)
                     /locus_tag="ACG8H8_002071"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP8987126.1"
                     /translation="MARTQKQKATPTHAVSLITLTNPSSPIAEQYRTIRTNIQFASTT
                     GQQIKTIVVTSSGPGEGKSTTASNIAVVFAKSGQRVLLVDADLRKPVIYKTFQLNNAS
                     GLSTALSSSGSVADVIQRAPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPIVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDDGYYYGN"
     gene            complement(7829..8608)
                     /locus_tag="ACG8H8_002072"
     CDS             complement(7829..8608)
                     /locus_tag="ACG8H8_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP8987127.1"
                     /translation="MEETISLEEIFETLRKHLITIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVQERLKTEDNVKMTP
                     EQIKDAISINQSENSQMFSIQAISTSAMTAQQIANTTSQVFQENAKDVMNVDKISIVS
                     GAVANTTPISPNNKLNLVIGLMLGMMVGVVLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSEKELKAGIRKKGLAPTRPKVTNDKTASSRRSRSRV"
     gene            complement(8624..9568)
                     /locus_tag="ACG8H8_002073"
     CDS             complement(8624..9568)
                     /locus_tag="ACG8H8_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8987128.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLNKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGVQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSILNALESNMKTDMSFDEMKKIALDYREAFDTIKQD
                     QMQGEGFMQDDISYQKVNDNELDRVKKELKEQMNLENK"
     gene            complement(9605..10381)
                     /locus_tag="ACG8H8_002074"
     CDS             complement(9605..10381)
                     /locus_tag="ACG8H8_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987129.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNIS"
BASE COUNT         3020 a         2230 c         1558 g         3603 t
ORIGIN      
        1 aaggtttaat cctgtaatta aactgatgcc aaatcttgca ataactgagt tctgcgtttc
       61 atctgactta gctgctccaa ttgcatataa tcctaaagtg ctataatcgc cttttcttaa
      121 tatttctacc aaaagaacac caaagaacac aacagataat agtgcaaaaa ttaaatttaa
      181 ttgtctgcta gggtaaattt gtacatattc aatcttatat tgattttgat ggtaattatc
      241 cactcttttt tttgataaat agtctaagaa taatatatac atttctggta tacacatagc
      301 taagaacccc gtcgtaataa accagagcgt cttcttgcta tattgcacat tccaaaattc
      361 gatattataa accacacagc atgtcgccaa aaaaaacaat gcaatcgcga taaatgctgg
      421 tgatataaaa catcgattag acaaaatata cattattaaa attagtataa attcaacaat
      481 caataattga taaatcattt attcttctcc taatattttc tttcggagaa tctccaaata
      541 ttcgcatccg aaaaccaaat ctggctccat ataatttcct tctccccact cattccaagc
      601 ctgaagaaat agtatccgat gctctggttc cttgtcttga acatacgaaa gtgctcggtc
      661 tattgcattt ccaaattttt ctggagtact atcaacatac actcttgcat ctttaccaga
      721 tcgtggggtt cgatctaatc ttggaatcaa ctgaggatgt acgcactctc tcttttcttc
      781 ttcacaataa aaattctcca ttatcttatc atatggatag agatttgctg aaaaattacc
      841 aactgtcttt cgcattatgc tcttaatacc tcttttccag tatcctgaaa tttttaattc
      901 ggcatatctc tgcatatcag aatttattgc gtcatatccc atatcaagga aacgctgaaa
      961 tctattatgc actgctacat ctgtcataga tgacaaatcg aacttattat caacagatgt
     1021 tactctagcc acaaaataga taccttcaaa tccattctcc ttagcaagtt tattccatag
     1081 tgaaataaaa tgagcagaat ctggaataga atctggagca aaaaccaaga ataaaggttt
     1141 accatcaact tttatatatc tatcatcctt caatgccgga agaatagatt taaagtgaag
     1201 tatatagtct tcatcaccag gatatgtttg ttccaaaaac acaacatcct ttgtgaaatg
     1261 ctttgacttt tgccaagttt tatttgacca tgaatgatta gcccaaccga gacaaaaagg
     1321 aaagtcaggt tctccgctct gtaacacctc attaaatgga agttctagaa ttctcttacc
     1381 attgccaaac caataatgcc aatagcaaaa accctcaacc ccatatttct tagccatctc
     1441 agcctgttgt tttcttactt ccgagattct taaatcatag aaacctaaat ctgcaggaat
     1501 ctggggttga taatgattct tgaatagagg tttagcttta gcaacattag tccattcagt
     1561 aaagcctttt ccccacatta tatcgttttc acgaacagga tgaaattgtg gcaaataaaa
     1621 tgccagtacc cttgcttttt tcatttaact gcctcctcta aagtaatacg atcaaaccaa
     1681 ttcagttttc cgccacgagt tgcgttatat atttttatgt tattactttc gcaaaactct
     1741 cttgctctta tatatgcctg attcattcca acaatacttg ctattacttc atttgcattt
     1801 ttgtcttcaa aaaaatgact tctaatggtc ttatcttcag acactgttcc atcagcatta
     1861 taagtcagtg cataactatg atccatacca agaagatata tttttttata tcccatataa
     1921 acagcagctt gaatcatcga ataagtaact gtttcatgcg atacaatgga tttactgata
     1981 tcctcactaa acttaatatt tttgccacca acagattgct ctgagtgaat acatatagca
     2041 tttggattat gcattcctct aactctccaa taataatccc caatgaacat gtattctaag
     2101 tcaatatcat ctaattcgtt accagtatct gcatatctat cctggataaa ataatattta
     2161 ggtctccact gggttttatc aaaaatctta aaaataagat tagcagcaaa acaatcctta
     2221 tcctttatta attccaaatc ctccattgtg agacttggac cattacctac aataaaacag
     2281 gtctcaccta aaaatttatt tctcagtttc tcaattcttt gaatattatc tgtattcttg
     2341 cagaacttat cgtagaagta tttgttttga acatcaacca caaatctcat tagctttaga
     2401 ataggcggaa ttctatgaat cacctttcta atttttatat ttaccatatt tactccttcc
     2461 gaattacaat atcaaccaaa aattcgttat ctaattaaat gtttactatc tctggccttt
     2521 ctcactaata ttcaatggag tgtcatcttc gaagatatac ccttcagctg atgcattacc
     2581 tttatattca ccaagtacct gtggccattc aataccaatc tcaggatcat tccaagccaa
     2641 gccgccctcg tcatttgcgt gatagaaatc atcactagta cctttcacaa cgatattcct
     2701 ttctttatta aacttttatc aactacgata attcaacttt tgatcgagtc ctcatcaatg
     2761 atactcttaa ttacatgagt caactgagtg ctggaaatac catctgtatg gggaagataa
     2821 acaaattcaa caccggttcc cataaactgt tcttcatatt tttcctactg aggattgcct
     2881 ttctaatcag aacccacgaa cattttgtta aatttatatt tctcccatgc cgcatactta
     2941 tccttatcat tttgagggac aaccatatcg acatacttta ttgaatttac aatttcggct
     3001 cgttctacat acggaataac aggtgtctta tttttatctt actaaaccgg ttcatctgta
     3061 ctaacaccga caatcaaaaa atcacattgt gcttttgcat ttcgcataac attcaaatgc
     3121 ccaatatgaa acatgtcata tacacccgtg gtgtattcta taactttttc tttcataaat
     3181 atctccttta actgtgtatt tcagagtaaa ataggaatct ttctcatcaa tctgcatcac
     3241 actctacatg tccaaataac atagaccgaa aagtcaagaa agataatttt ccagtctgtg
     3301 atgaaccttc tctcttctac atacttcaca tagagttcca ttcattcttc gaaagataaa
     3361 tcatttcgac gcgtaacaat cattaccatc gaacaaaaac gttgaaccac atacaacagg
     3421 cacattcctg cggtaatttc tttaatgttt tttctcctga gtcttcactt accccgatat
     3481 agttaatgcg actcaaaaaa tttcttgctt tcatcgttaa cctctctttt taactaacat
     3541 ttcaaacaat accgcatgtt ctgaagctac ccttttggaa ttatactttt caagcactac
     3601 cgatgtagct tcggaagtta gccttgcagc cattgcttta tccgaaaaaa tcttaaaaac
     3661 tgcttcgcaa gcagccgtct catcatcaac gggcaccaac aatccatttt ttccattgct
     3721 aatcaaattg ggaattgcat caatattggt ggcaatgata ggttttccca ccatcatgta
     3781 ctcaggcaga gccaaaccaa atccttccca acggcttaat aaacatgcaa catcaaatag
     3841 ttcaatatag cttatcgaat tatttaccca gcctgtaata tgcagacttt ccaaaaaacc
     3901 atgttctgct gcatatttct cgataagtgc tttttgctct ccattaccaa caataatgaa
     3961 atgggcctct ggtattttat tttttaccaa tctagccatt ttaatgaata tttctggagc
     4021 cttctgcgga ctaattcttc caaccatacc tacaacaaac gcatcttgtg gtatattcaa
     4081 actttctctt tttacctctc catgagcttt atttctatat gcctcaatgt ctacgccatt
     4141 aaaaatgacc tgcaacttat cttcgcaaca aattctcttt ttcagtgctg attgtttctc
     4201 ggcatctgat atacagataa ttttatcgca aaacagagca gctatttttt ctatagcagt
     4261 gtatagcatt ttcttcttaa ctgagcacct catgttgaat gcccatccat gtggattgta
     4321 tacgcaatag ttcttcaaac caattttagc aacacgtgta atggctcctg ctttactcga
     4381 atgtgcatac acaatatccg gattatattt ctttatcagc tttctaataa ttacgattgc
     4441 ttttaggtca ttgagatcaa tttctctagt caatttaatt tgcttaaatg agagcacaag
     4501 atttgcataa tccgtctcgc aaaaatcttg cgagcaaatc aatatgtttt caaacttttc
     4561 cttatccaaa tactttaaaa gcatgcgaat ataacaatct acgccacctg cagcttgggc
     4621 aatgtgtaaa actcttattt tttgacgcat ttctgtcctc cattttactt atcataacaa
     4681 acagtatatc tcgaattacc agaaaaataa tcttccaatc agcaaagaaa actctcttat
     4741 actacttcac attgatctcc atccattctt taaaggaaag ttcatttcaa ttcagagcaa
     4801 tctgccaata atagctaaga tcaggctgta catacaattg ctgtctttca tagcctccat
     4861 actgcttcat ctttctcagc aatccagatt tgggacccac gatattcata tcttccttga
     4921 aaatattcca aatctgagga gattcatcat ccacgaacac gaaacgaaac ttatagaact
     4981 tgaacaattt accatttctt ccaactctat cttgtgcgaa aacaggagtc cgccggatca
     5041 tcaaacacaa taactaacgc tgtaatcagt atcacaggac acaaatagca agtgttaaaa
     5101 tggaaaatat gaatacttct ccaatatacc cggactagat aactcccttt tgttcataat
     5161 ccctcatact catcagattc accaaacttt accgtttatt tttttcaata gttcaccaaa
     5221 tgactccatc taacaaggtc aaatataaaa aattaacgca ttttcattat cttttaggca
     5281 tttattaatg gagatattta gtgtttttat aacataataa atgctcaatg agagcctatg
     5341 ttgggtacat caatattaaa gtattcagca ctaatatgtt taagatcgat attatatatt
     5401 tcataatttt gaaaattttc tttcatatag gaaacatgat actgaattgc tttagtcacc
     5461 tttctttttt tagattttct acaagtgatt caaaacttcc ataattacag ttattacttt
     5521 tgaaacgata atatgaacat gtgtaatctt ttttatcaac aatctatcaa ttctatccct
     5581 tactaatatg ccccatttgg ttttataacg atccaaattg tcttgataat tagtttaaaa
     5641 tcattcaata gactttgatt agttatatat tctaaatcta aatcaaccat tgcatgaaaa
     5701 ctaacatcat ttctaccact aacttgccac aaacctgaac atccaggttt tacatacaag
     5761 cgttgaagat catatctcgt gtactcagct acttcccttt ccaaaggtgg acgtgggcca
     5821 actagactca tatctccttt taaaacattc catagctgtg gcaattcatc aatgctttta
     5881 gcgcggataa ttttacctat ttgggtgaca cggggatctt ctttcatctt gaacattgcg
     5941 ccttctattt cattttgatc aagtaactct tccaattttt cttcagcatc taaacacatc
     6001 gaacggaatt tatacatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa
     6061 aatatcggac tccctggctc ttcttttctg attcgataag ctgcatagaa catcaaagga
     6121 gaagctaaaa tcaaaccaat gatgcttcct acaatatcaa tcattctttt aaaaaataaa
     6181 tagatatatc gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttta
     6241 ctaatacgag ataataaatc ttcactttcc atactttcag tccctttcgc tctctattca
     6301 tttccttttt atctacgaat aaaaactaaa ataaaccaaa cttcttcttt tttacctctt
     6361 catatattgg ataactgatc tcatccccgt tgatcagatc ctttgcgact tgcttcatct
     6421 gtactacttt ttccttacca aagtccttgg caatctgctc atagcactct tttaagtaaa
     6481 aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga ttatgtttga
     6541 ccatcgtttt ggctgttttc tggatatctt tgccaaatac cccaacataa cttggcgcag
     6601 tgagttgcgc caaacatccc atatctagga acggaagcaa atgattcggg tcttcgcgaa
     6661 aatgcgcgtt acgttctgga tggacgatca ctggtgtctt tcctagttct aacaactcaa
     6721 aaaaaagctg ctctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     6781 tatcatctaa atctgtaaat aaaatttcat ctcgctggat gtcttcaatg agcgttccgg
     6841 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ttgtcttttc tctaattctg
     6901 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     6961 tgtggtgtgg cgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccatta
     7021 cgatacttgc ctctaggttt tccgccccat catcgattcc tggtaggata tggcaatgta
     7081 gatcgatcat ttctcttgcc tcctctcttc ttatttctta atttccgtag taataaccat
     7141 cgtctttgct gtgttctgct ccgttgtata caacacctag gactcgtgct tgtaccatct
     7201 ccaacagctc ttttgcttta gtcaaggatt ctttacgtga cgtattttca cgtaccacca
     7261 ataaggtgcc atccgttttt gaagacatga tctgtgcatc tgttacggct actattggtg
     7321 gcatatcaaa aattaccacg tcaaataact gtcttgcttc tgctaggatc tgatccattc
     7381 ttggcgaact taacaattct gatggattgg gtggcttagg tccactaggc aaaatcgaca
     7441 agttctcgac tggcgctcgt tggatcacat ctgctacact tcctgatgaa ctaagtgctg
     7501 tacttaatcc actggcattg ttcagctgaa atgtcttata gatgacgggt ttacgtaaat
     7561 ctgcatcgac tagtagaact cgttgaccag atttagcaaa aacgactgca atgtttgatg
     7621 cagtggtaga ttttccttct ccaggacctg atgatgtcac gacaatcgtt ttgatttgct
     7681 gaccagttgt ggaggcaaat tggatattcg tccggatcgt acgatattgt tcggcgattg
     7741 gtgaagatgg gtttgttaac gtgatcaaac tgactgcatg agttggtgtt gctttttgtt
     7801 tctgtgttcg tgccattttt cttccctcct aaactcttga gcgacttctg cgtgatgagg
     7861 ctgttttatc gtttgtaact tttggtcgtg taggagccag tcctttttta cggataccag
     7921 ctttcaattc tttctctgac atttctggaa ctgttcctaa gattgggaac cctaatgtat
     7981 ccgtcacata tttttcatct ttcactgttc gatcgagtaa ttcaaataag aatgccagaa
     8041 ctacacctac catcattcct aacattaagc caatcactag attcaattta ttatttggcg
     8101 aaataggtgt cgtattggca actgcacctg aaacgatcga gattttatca acattcatca
     8161 cgtcttttgc attctcttgg aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca
     8221 ttgcactcgt gcttattgct tgaatcgaga acatttgtga attttctgat tgattgattg
     8281 agatggcatc cttgatttgt tcaggagtca ttttcacgtt gtcttctgtt ttcaaacgtt
     8341 cttggacttc atttagcact agatcactga cgatcatatc tttatacgta ttgatcattt
     8401 gcaagttcgt attgacatca ttgacatttg cagtatctga ttgcggcagt gtcacaatca
     8461 actgtgcttg cgaactgtat tttggcgtca aaatgaaaaa cgttacaatt cctgagatag
     8521 ccaagccagc aaacatcgag atgatgattg ttatcaagtg tttgcgtaat gtttcaaaaa
     8581 tttcttccaa gctgattgtc tcttccattt tttctccact ttcctattta ttctctaaat
     8641 tcatttgttc ttttaattct tttttcacac gatcaagttc attgtcatta actttttgat
     8701 atgagatgtc atcttgcatg aagccttctc cttgcatctg atcttgcttg atcgtatcaa
     8761 atgcttcccg ataatctaaa gcaatctttt tcatttcatc aaagctcatg tctgttttca
     8821 tattcgactc tagagcattc aaaatggatt gatagttgct gacaccattt agactcaata
     8881 ctttttttgc tacgccttct acgatctttc tttggcgccc ttgtctaccg taatcaccat
     8941 ttgggtcttc ataacgcata cgagaataag ataatgcctg aaccccgttc aatgtaattt
     9001 taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac
     9061 caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca tagtgatcga
     9121 ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt
     9181 aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag
     9241 gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat
     9301 ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc aatagaacag
     9361 agaaagattc ttgtttgttc agatcaactg gtttcgttcg ttctccttgg gttcgttcca
     9421 ctgactcgta agttttctca atagaatgtg agagatccag atataatttt gctcctactc
     9481 ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat atttttttcc
     9541 ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctagctt tgctttaatt
     9601 gttactaact tatattgcta tagcccattt gctgagcaac agtttgtgct tttgttgcgt
     9661 atgctgcgcg tattttttca ttccggatct gctctatttg actgagcacc gtcaaatatt
     9721 gatcgtaagt agcggtggca gtcacttgac catccttcaa catcgtatcg aataactgat
     9781 ctagttgcgt gacttgcgcg atctgagtat cagcgaatcc tagatattgc ataacaaccg
     9841 ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac
     9901 ttgtcacctt ttctttgatg acgacatcgt ctacagcttg ctgccagttt gatacatttt
     9961 tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca tcctctaact
    10021 gcgttaagac tgctttcttt tcttcttgga tcgcagcagc ttctttaggt aattctgatt
    10081 ccttgatgtc ataatcatct gccgaaagtt tgatagaaga tagtttatgt gacaaatttg
    10141 aaactgtttc ttctgttatt tcaggattta ggaaaatttt ttcttttgta ctgtacagct
    10201 tatctacttc ttttgataaa gaggtcacgg tgtctttttg attggtgatg gcagattctg
    10261 cgtaattgat ccgttgatga tgttgccgct gattattgaa atggtatcca aacaagcaaa
    10321 tcacaatagc gcctgtgcct accgccacag tagccaataa cttgacgaac ggcttttcca
    10381 tagattcact cctaaatatt gatgatagat a
//
LOCUS       DAZKAO010000094        10233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000094 DAZKAO010000000
VERSION     DAZKAO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.094"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..228
                     /locus_tag="ACG8H8_002075"
     CDS             79..228
                     /locus_tag="ACG8H8_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP8987130.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            239..1753
                     /gene="dltA"
                     /locus_tag="ACG8H8_002076"
     CDS             239..1753
                     /gene="dltA"
                     /locus_tag="ACG8H8_002076"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP8987131.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1750..2955
                     /gene="dltB"
                     /locus_tag="ACG8H8_002077"
     CDS             1750..2955
                     /gene="dltB"
                     /locus_tag="ACG8H8_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP8987132.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3000..3233
                     /gene="dltC"
                     /locus_tag="ACG8H8_002078"
     CDS             3000..3233
                     /gene="dltC"
                     /locus_tag="ACG8H8_002078"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP8987133.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3236..4504
                     /gene="dltD"
                     /locus_tag="ACG8H8_002079"
     CDS             3236..4504
                     /gene="dltD"
                     /locus_tag="ACG8H8_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP8987134.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4645..5727
                     /locus_tag="ACG8H8_002080"
     CDS             4645..5727
                     /locus_tag="ACG8H8_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP8987135.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5727..6530
                     /locus_tag="ACG8H8_002081"
     CDS             5727..6530
                     /locus_tag="ACG8H8_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP8987136.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6600..7340
                     /locus_tag="ACG8H8_002082"
     CDS             6600..7340
                     /locus_tag="ACG8H8_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8987137.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7351..8355
                     /locus_tag="ACG8H8_002083"
     CDS             7351..8355
                     /locus_tag="ACG8H8_002083"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP8987138.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8400..>10233)
                     /locus_tag="ACG8H8_002084"
     CDS             complement(8400..>10233)
                     /locus_tag="ACG8H8_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987139.1"
                     /translation="NNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSN
                     QMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGN
                     HQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLP
                     PKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLS
                     CFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGI
                     FCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVA
                     CTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDR
                     RQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEK
                     ILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQL
                     LIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELD
                     DQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3279 a         1696 c         2070 g         3188 t
ORIGIN      
        1 tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt
       61 atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa ttattgggga
      121 aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat ttatctgtat
      181 cactataaaa atattcaagg aggaactttt atctataacg aattttagga ggaagagtat
      241 ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac ctgatcgagt
      301 tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat cagacgcact
      361 tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt ttggcaattt
      421 ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg cctatttgcc
      481 gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc
      541 tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg tcataacaaa
      601 aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa atagcgtaaa
      661 aggtacagaa aatttttata ttatttttac ttctggtact actggagaac caaaaggcgt
      721 acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag actttgggat
      781 caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat cggtattttc
      841 tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag cagttgttca
      901 agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt gggtttctac
      961 tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa atgtgccgga
     1021 attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag aaagtctact
     1081 tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag caactgtagc
     1141 tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac taccgattgg
     1201 ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg
     1261 agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc
     1321 cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc atacagggga
     1381 tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg atcagcaggt
     1441 caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc tccaagacaa
     1501 ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac agcagctggt
     1561 tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga ctaaatctat
     1621 caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt tccattatgt
     1681 cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt
     1741 gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta tttttttctt
     1801 ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa aagattacca
     1861 gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg taattattgg
     1921 caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt atggggctat
     1981 tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt tattttagcg
     2041 ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa agcatcgtta
     2101 cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat catggaaata
     2161 agagatggca tgatcaaaac atataagcca tttcgttata tccagtttct gctgttcttt
     2221 ccaacaatct cttctggacc aatcgatcgc tataggagat ttgaaaaaga tcagctccat
     2281 ccgcctgttc gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc
     2341 ggttttttat ataaattcat tatcggttat tattttggtc aagtactgct tcctgtagtg
     2401 ggacgcattg ccttacagca tggtggtatt tcatgggcat tagtaggtta tatgtacgtg
     2461 tacagtatgt atttgttttt tgattttgct ggctatagct tatttgcagt cggtacaagc
     2521 tacatgatgg gatatcagac accaatcaat ttcaataaac cttttttatc atggaatatc
     2581 aaagaattct ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga ttatgtatat
     2641 atgcgattga tgttcttcat gatgaagaaa aaatttttta aaagcaagat cgtaatgtca
     2701 aatatcggtt attttgcttt gtttcttcta atgggtatat ggcatggatt gacgtggtac
     2761 tacatcgtat atggactgta tcatgcttgt ctgatctgtc tgacagatgc atggatccga
     2821 tttaagaaga agcataaaga gcagttgcct tctaacaaat ttacgcatgg cttagccgta
     2881 tttttgacat ttcaagcagt atgttttagc ttcctgattt tttcaggatt tttagataaa
     2941 ctttttttta gctaacgaat agtgacatat taatttatat aacaaaggag aaaaggaaaa
     3001 tggaagaaca aattttatca atcttagaag aaataacagg aacagatgaa gtaagaaaaa
     3061 atcgtgatat tgatctattt gatgaagggt tgcttgattc tttagcatct gttcaattat
     3121 tagtcgagct agacggagag ctggatatcc aagtccctgt atctgaattc gagcgagagg
     3181 attggtgcac accgaataaa atcattcaac aagcaactgc attgaagggt tgagtatgaa
     3241 aaagaaactt tttgctattt tcggcccggt tgttttagct gttggcttac tgtttgtgtt
     3301 ttttacctcc tcctatcaag tagatatctc taatccagaa acgattagac gtgcctcttc
     3361 ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa
     3421 acaatatatt cctttttttg gctcaagtga attaagcaga atcagtccat ttcatccgtc
     3481 tgttctggca gaaaaatacc agcgaaacta tcgtccattt ttgctgggag cacccgggac
     3541 acaatcgtta agccaatata tgatgatgcg ttctgcagga gatgctatga aaaacaagaa
     3601 agtcgtcttt attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt
     3661 taatacgtat tattctgaac ttcaaacata cgactggttg ttttcaatga agaaagtaac
     3721 gcctgctgat cgctatttag ctagaagatt attaactttc tcaaaagtaa aagagaatga
     3781 tacgttgacg gcgattctgc aaacgatcaa aaaagggaag ctacctttac ctgaatcttt
     3841 gaatcagctg cgcagccaat ggaacatgct gaagagagag gatgaagtat tcagcaatat
     3901 tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca aaacgattac caaaacaata
     3961 ccaagaaaca gaattatcca ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa
     4021 tccattcggg ttgaaaaacg atttttatac gcatcgtatc cgagcccacg agccagagct
     4081 caaacagtca caaaaaaatt gggattatcg tttttcgcca gaattttctg attttcagct
     4141 ggtcttggat caattagcaa aaaaccataa cgaagtattg ttcatcatcc caccggttaa
     4201 cgagaaatgg agcgattata ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa
     4261 gatcaaattt caattaaata gccaaggatt caatcgaata gctgattttg tcaatcaagc
     4321 gggtaccaac tattttatgg aagacacgat ccatctaggc tggaaaggct ggttggctgc
     4381 agatcaacag atcagaccgt ttttagaaga aaatcatatt actgctagta agtatcatct
     4441 tgacgatgct ttttttagca aatcttggca acatcaaata ccagataaac tacaattaaa
     4501 ataaagaaaa caggcgagtg ttgctcgctt gttttcttta tttttgaata ttctgaataa
     4561 aagataaaaa taccaagttt gcctgaacaa aaatcataag caatggtata atcacaatat
     4621 gaatatagaa atgggggaaa acatttggat acaaaagaag aacagctact tattgattta
     4681 acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt cagagaatat
     4741 gccaaaccat tcgctgatga tattttttat gacggtttag gcagtgtgat tgccaaacat
     4801 ggtgctggcg gaggccccaa ggtctttatt tctggacata tggacgaagt tggttttatg
     4861 gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa ctcttggagg ttggtggggg
     4921 caagtcatgc ttgctcagca agttgaaatc aaaacacgtg aagggaaaat cgtccacggt
     4981 gtgattggtt ccaaaccacc acatgtttta acacctcaag taagaaataa accttatgaa
     5041 ataaaggata tgttcattga tattggcgca tcaagccaag aagaagccaa agagtggggc
     5101 atacgtcctg gagatatggt cactccatat attgaataca aacgaatgaa tggcagtaaa
     5161 tatttactgg caaaagcatg ggataatcgt atcgggacag ctgtttctct gcgagtatta
     5221 gagaatctat ctaaagaagc acacccaaat gttttattcg caggaagcga tgtgcaagaa
     5281 gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg taaatcccga tattgctttt
     5341 gctttagata ctggtacagc aggagacact ccaggaatga caccaaaaga agcagattcg
     5401 attttaggaa aaggcccaca gattttgatt tttgatgcat ctatgatccc gcataaaaaa
     5461 ttattgaatt ttgttattga tgtagcagag gaaatggata ttccattcca atatacagtg
     5521 atttctggcg gaggaactga tgcaggacaa atgcatttga caagagatgg tgttccgtct
     5581 gtagccatta ccgttcctgt tcgttatctg cattcccata catcagtgat tcatgaagat
     5641 gattatttga atacagtgaa actagtcaca gaattagtga aacggcttga tagtgaaaca
     5701 gttgaaaaaa ttcggagtta tgaataatga ataatagaat acctatttct gaatatcgga
     5761 aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa tacttggctt gttttcttat
     5821 ctataatatt tacgagcgtc atccagaact tttttgacgg tttcagtcct tttccggaag
     5881 aaagcatgca gcagcactta ttgagttttc tagtcgataa tttactttta ttcgcattca
     5941 actatgcatt gttttacatt gcgctagtag ttgtacgggg tggcagacca aaaagtaacc
     6001 ttttattttc agttttccaa agaaagtatt ttggttcgat acttggcatc aatttgatca
     6061 attcagtcat caactggcta ctcaacagtt taatctttct gcccatgttt ttaatcagcg
     6121 gagtgagtac ttatatgcag ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc
     6181 cagaagattt tcttgatatc agctttgttt tatcgatgat agggatgatt tttgtttcag
     6241 cactcatcat gagtattgtt ggcggtttat tccaatttgc tgcatggaca aagttcgact
     6301 atcctgattt gtcggttatt caatgtttga aatatggctg gtttttattg aaagatagaa
     6361 ttggtacgta tattttgttg cagctgtctt ttatcggctg gtatttgcta ggagcaatcg
     6421 ctttgttcat cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt
     6481 atgaacaagc acggattgaa aaaggaggtc cagacgccta ttttacttag catgtaagca
     6541 gtttattcat caaaaaaata acttttgtgg taggataaat ctttgagggg gaatgtacga
     6601 tgaaattaac tgttttagga tgcttaggag cgtatcctta taaaaatcaa gggacaacag
     6661 gttatttgct tcaatctgac gggttcaatt tgttgatcga tgcaggtagt gctacgctga
     6721 tcaaattaca agaacacctt gatccacttg atctagatgc tgtgatcctt agccattatc
     6781 atcatgatca tattgctgac cttggtgtat tacagtatta ctggcaactt catcctaaga
     6841 gaaatccgga gacattgctt tcaatttatg gacacacaaa agattccatt cactttgaag
     6901 aattgacgtt ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa gaattaaaaa
     6961 ttggtccttt tctgattact tttatggaaa caatccatcc agtagtatgt tacgccatgc
     7021 gtatcgtaga agaaacaacg gggaaagtat tcgtatttac aggtgattca ggttatttgg
     7081 aaagtttcgc tgattttgcg aaagatgctg atttgtttct agcagatacc tatctttttg
     7141 aaggaaatga acatcatcat gcccatttca cttcaaaaga agctggagaa cttgcgaaat
     7201 cagctaacgt aaagaaactt gtacttacac acctacctca gtttggtgat ttagaacaat
     7261 tgaaaaatga agcgcaaaag gcagcaggca aagggattga tatagaactt gctgcagttg
     7321 acaaagtgtt taacatttag gaggaagaac atgatttttg taccaaatga aaaccacgac
     7381 ccaagaatca atttagcaat tgaacaatat ttgctgcaag aaatgccttt agatgaacct
     7441 attttgctct tttatatcaa tgaaccttca atcatcatcg gcagaaacca aaatacgctt
     7501 gaagaaatca atcgagatta tgtggaagaa aatggcattc atgttgtccg tcgcttaagt
     7561 ggaggcggag ctgtctacca tgattttgga aacctgaact ttagttttat catgcctgat
     7621 gatggggatt cattccgtga ctttgcgaaa gtaacacagc caatcatcca agcacttcat
     7681 gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg atctagtcat tgatggcatg
     7741 aaattttctg gcaacgcgat gtatgctacc aacggtcgga tgtttgctca tggaacaatt
     7801 atgttcgact ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc
     7861 gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga atatcaaacc atttcttcca
     7921 gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc
     7981 ggcgtagagt cagtagaaga agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa
     8041 atcaatcgaa tctctgagga atattatcga aattgggatt ggaattacgg gaagtctcca
     8101 gatttcaatt tctctcgcca aaaacgtttt tctatcggtt cgattgaagt ccatttgaat
     8161 gtctcagaag gaatgatcaa agaagcaaaa atctttggcg atttcttcgg actaggagat
     8221 atctctgacg tggaaaaaaa attggttgga cagaaatatg ataaagcatc tctatcaaaa
     8281 atagtcagcc aaattgatgt gaagaaatat tttggtgcga tagaaccaga agaattgctt
     8341 accttacttt attgaaaata aaaaatagcg gaatccacat atatggattc cgctattttt
     8401 tatacaggat tcgggtaatt tcgagagagt tgaacagtga tccgatcaat gaaagtatct
     8461 ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat cattaagtca
     8521 aaacgttcct gatcatctaa ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa
     8581 tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa
     8641 atttttcgat acagtggatt ttcgatggca tgagtttctc taaaggaatg ctctaaagcg
     8701 tggatcaaaa gttgagccag atcgtcatcg ttgataggaa gttcttcggg aagatctgct
     8761 tggaaggtta tctgaatatt ccgtttggca gctaacttct ggtaacgcgc taagataaga
     8821 tttgtcacct ggtggttgca aaaagaacac agcatttcga aattttttgc ttcttctaat
     8881 atcttttcca catagttttt agcttttgtt tggtccgtat gcattaaatc ttgcagattc
     8941 tccatctctt ggacaaattc caggcgaagt gtatttactt cttggatatg ctgtcgaagc
     9001 tgctcataat gttgctccaa caaatcattt ttgattttta agaaatctac ttgtctccga
     9061 tctttgtcgg tatttaagat gtgatcgatt agattgtagc tgataataac gattagaatg
     9121 ataatatata aaatcgtaga ccaattgatc gttgtttgat agatacctaa tgccgtttgg
     9181 atgtaaatca agcaatggat aatggctacc aagacgatcc aaggtgtaca agctacataa
     9241 aaacggttat tcttatatgc ttcgccaatg catgcaagtg aagtgactaa agtgcttata
     9301 acactagcaa tattagcaat cggcattaat tcaggaagtt ctgcaggagt aaaagttgca
     9361 taaagcagaa cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt
     9421 ttatagtaaa acattcggct ccaatagtag cagatgatga aagctgacgt taagataata
     9481 aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa gaataccact taaaatatct
     9541 tctgatacag cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat aagtgagtat
     9601 tcaagacgtt tttgtttata catcaagtag atcgtataga aaaaggtccc tatagcgata
     9661 aaaacagcga ttatcatcgt caatacttgc ggcactgatt gtgaataaat aaaactaatc
     9721 aatggtccag ttgttcctag ataaactttc ggtggcagtc cagcatagtt ctgataagga
     9781 gatgagactg taagggtcaa tttttttccg atataatttg ctggtaaatc tatcacagcc
     9841 aatgagagtc cgggattgct ttcttcctga taatctttcc gttggtataa aatcttttcg
     9901 cctagatata cagtaatcca ttgatggttt cctttgatta gtaaatttgc ttcgtccaac
     9961 ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt tatccacggt aggaatcgta
    10021 cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa aacccacgaa
    10081 tcttttattt ctttcatttg attgctgtcc gcaggagaaa aaataaaata aaagaaataa
    10141 gcaattcctg ttaaaaataa caatgataat ataatgatta tgctccactt tatacgtaaa
    10201 taaaaagatt cttttttatt atatatgtta tta
//
LOCUS       DAZKAO010000095        10092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000095 DAZKAO010000000
VERSION     DAZKAO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.096"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(556..780)
                     /locus_tag="ACG8H8_002085"
     CDS             complement(556..780)
                     /locus_tag="ACG8H8_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987140.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            971..3748
                     /locus_tag="ACG8H8_002086"
     CDS             971..3748
                     /locus_tag="ACG8H8_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8987141.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3829..4248
                     /locus_tag="ACG8H8_002087"
     CDS             3829..4248
                     /locus_tag="ACG8H8_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8987142.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4260..4730
                     /locus_tag="ACG8H8_002088"
     CDS             4260..4730
                     /locus_tag="ACG8H8_002088"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8987143.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4747..5526
                     /locus_tag="ACG8H8_002089"
     CDS             4747..5526
                     /locus_tag="ACG8H8_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8987144.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5513..6334
                     /locus_tag="ACG8H8_002090"
     CDS             5513..6334
                     /locus_tag="ACG8H8_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8987145.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6351..7400
                     /locus_tag="ACG8H8_002091"
     CDS             6351..7400
                     /locus_tag="ACG8H8_002091"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8987146.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7413..8417
                     /locus_tag="ACG8H8_002092"
     CDS             7413..8417
                     /locus_tag="ACG8H8_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8987147.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9050..10069
                     /locus_tag="ACG8H8_002093"
     CDS             9050..10069
                     /locus_tag="ACG8H8_002093"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP8987148.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3390 a         1658 c         2100 g         2944 t
ORIGIN      
        1 tataaatgaa ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt
       61 acttcttttt taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg
      121 gttgagagaa atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt
      181 cgtttattgt gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc
      241 gcagattcta tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac
      301 ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc
      361 ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg
      421 gtgacaacag tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc
      481 aataaattgt tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt
      541 tgaaatctgt gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa
      601 gatgaataaa aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg
      661 ttctgtcata gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat
      721 gatttctcga aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat
      781 tgaatagtca tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag
      841 gaactgtacg ttttttagaa taaaaattat aagggataag taggctgctg tctataataa
      901 gatatatttt tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga
      961 tggaggaggg gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac
     1021 agcctttttc gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca
     1081 ttttggggta aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact
     1141 actagtcaaa atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca
     1201 attttttaag ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc
     1261 ttttttcgcg caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa
     1321 agaaagtatc gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt
     1381 gattacaggc gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg
     1441 tctgcttcat gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata
     1501 tgcagataat cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac
     1561 tggtgcagaa gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt
     1621 agacgaggta catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca
     1681 aggtgtgatt tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt
     1741 ttttgctacg actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc
     1801 tatccgaatc gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat
     1861 ttactctttt tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc
     1921 agctttgtca cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat
     1981 cgtcaaagtt tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt
     2041 cacgatccat catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca
     2101 gcaaggaatc tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc
     2161 cgaacagcag cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa
     2221 ctgtctgcta tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt
     2281 tttgctgttt gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc
     2341 tatccgagag acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag
     2401 tgtctatgcc ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga
     2461 acatgatatt atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc
     2521 tagttatcac tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga
     2581 cacaagtatg gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga
     2641 aaaacgactg ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc
     2701 aaatgtagta aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga
     2761 tgtcacacca cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc
     2821 aaatggattg atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa
     2881 aaaacagtta tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac
     2941 agtaggggaa tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg
     3001 acagatccag ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat
     3061 tactacttgc ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag
     3121 cttacctacg gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa
     3181 gaaaaacagt gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga
     3241 tcctgatatc accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat
     3301 gtctgtacta gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa
     3361 tcagcgcatc attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga
     3421 tacagagaag gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa
     3481 tcgaagcatc agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga
     3541 acgattgatc cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg
     3601 tcaaaaagaa gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag
     3661 tgtcatgatc cccaattctg agattgccta tatctatgat attttgagtg aagatacaga
     3721 gtttctctcg atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca
     3781 tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa
     3841 attatcttag cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc
     3901 tatgggaaaa atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta
     3961 gcggaaacag taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg
     4021 gatctttttg gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc
     4081 tatctcgttg ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct
     4141 gaagtagaga caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag
     4201 ttttgtaacg agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa
     4261 tgattgtatt gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga
     4321 cacaaacaat aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga
     4381 tacgaaagac aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa
     4441 ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg
     4501 tagtggaatc gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca
     4561 tcaacttagg agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata
     4621 ttttaccaga agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa
     4681 tccgtcaagt accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga
     4741 acctcaatga ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa
     4801 tatgcactag ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc
     4861 gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc
     4921 ggatcgttct cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg
     4981 acggcttttg ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg
     5041 attgctacgt tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg
     5101 aaccataaag cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac
     5161 ttgcttgccg gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg
     5221 gtgggaagca atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg
     5281 ttgtcagtag ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg
     5341 atcaacaaac aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag
     5401 attcctgtga ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt
     5461 atgaatgtcc gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa
     5521 ttctgagatc aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc
     5581 ttttcaaatg gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc
     5641 aatgattccg attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa
     5701 acggcatcta gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac
     5761 ggctgcaatg gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag
     5821 tatcaaaaca tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac
     5881 tttgcgattg attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg
     5941 ccctatcttg ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac
     6001 aggttgggga tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat
     6061 ggaaagtttg acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc
     6121 cacgatgatc accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt
     6181 tcaaagtatt tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt
     6241 gttttatcta ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt
     6301 tggaattttt ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga
     6361 tgcaagatta tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt
     6421 tttcgacaag agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat
     6481 cttataacgc ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg
     6541 atattcaaga acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg
     6601 tgattgctgt gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac
     6661 aaaaacaagg gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag
     6721 aagctgcaca aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag
     6781 gctattctgc gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa
     6841 ataaaatcag caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc
     6901 atttaccagc gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc
     6961 gtttagcaca gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag
     7021 cagagacgaa attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt
     7081 atatgcacgg cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg
     7141 aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag
     7201 agactctcac cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag
     7261 aatgtgatga atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata
     7321 aaaccgcagt agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag
     7381 tcttgaaaag taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa
     7441 tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac
     7501 tttatgcgga aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct
     7561 cgatgcaagc tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg
     7621 ctgcagaata ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt
     7681 cctctgttac cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag
     7741 gagcgacact gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt
     7801 ttgtggtcac ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc
     7861 gattgatgta tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac
     7921 gttatttacc aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat
     7981 ttgcagaaaa acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg
     8041 gagctgttta ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat
     8101 ctatccatgc tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag
     8161 tcacattatt tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt
     8221 tgccaaaaat ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta
     8281 tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac
     8341 gcatcgatgc tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc
     8401 gtcagtttga ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg
     8461 ataactgctc cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt
     8521 ttgcattatc tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt
     8581 ttttgttatg caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac
     8641 aacgagttgc gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt
     8701 cctatgtcaa ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat
     8761 gttcaatctg tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc
     8821 ctaatagtaa tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc
     8881 ataacgagtg taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa
     8941 aaacgataaa agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga
     9001 aatgtatact ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa
     9061 aatcgcagtt cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa
     9121 cgggcatgat gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta
     9181 tcatacaaat cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa
     9241 agaattggca gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc
     9301 gattcgttca gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca
     9361 tgcaagtaaa ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga
     9421 aattccagaa gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga
     9481 agtggcagta catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc
     9541 ctatgtacaa aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat
     9601 tggcgtagaa actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca
     9661 tggattagga ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat
     9721 cagccgtctt ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt
     9781 gggggacttg atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt
     9841 gttaggacag ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg
     9901 tgtggcaacg acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat
     9961 tactcaaaca atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga
    10021 aatcatgtta cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca
    10081 atagttaatc gt
//
LOCUS       DAZKAO010000096         9968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000096 DAZKAO010000000
VERSION     DAZKAO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.097"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1729)
                     /locus_tag="ACG8H8_002094"
     CDS             complement(143..1729)
                     /locus_tag="ACG8H8_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP8987149.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            1980..2204
                     /locus_tag="ACG8H8_002095"
     CDS             1980..2204
                     /locus_tag="ACG8H8_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP8987150.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2346..4097
                     /locus_tag="ACG8H8_002096"
     CDS             2346..4097
                     /locus_tag="ACG8H8_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP8987151.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            4097..5881
                     /locus_tag="ACG8H8_002097"
     CDS             4097..5881
                     /locus_tag="ACG8H8_002097"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987152.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6204..7055
                     /gene="licT"
                     /locus_tag="ACG8H8_002098"
     CDS             6204..7055
                     /gene="licT"
                     /locus_tag="ACG8H8_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP8987153.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            7152..9038
                     /locus_tag="ACG8H8_002099"
     CDS             7152..9038
                     /locus_tag="ACG8H8_002099"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8987154.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9079..>9968)
                     /locus_tag="ACG8H8_002100"
     CDS             complement(9079..>9968)
                     /locus_tag="ACG8H8_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8987155.1"
                     /translation="KIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYVHPSTL
                     IRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMASLEQM
                     TIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDVTIVTT
                     FGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDSTLSSMS
                     TAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQKFDKR
                     PISSAIMKEPFNFENKEVD"
BASE COUNT         3143 a         1786 c         2114 g         2925 t
ORIGIN      
        1 atacacggtg ttccagaagt aacttcttcg gaaataagcc gaaatttcac aaaaatttga
       61 aaagcaattt tcttaaattc cttcttattt cttgaagctg cccacttctg tcacaacctc
      121 ctttgtgtaa aagaatcagt ttctattcga tttcttctat cgtttcttgc aaacgctgtt
      181 catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa
      241 gaacaatgat tacataggcg atttgatcag ccaatgctag ttgagctgag gtggcttgct
      301 cgccgaagaa actttcgatt tgacctggta atcctcgtaa gttcaagtaa gtcagactaa
      361 tcacagagaa ccagccaaga agttttacaa caaatgaatt tttgaatcgg ctccccatct
      421 ccgcttggct attggtcatc atcagtaaag gaatcatcga gaatggcaat gcaaaagcca
      481 gaaaaacttg ggattcattc atcaagttat tcaaagcggt atgttcttcg atcgttcctt
      541 ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacta
      601 gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg acttgaccgg
      661 tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag
      721 tagacaagat accagatttt gctacagcaa tcaagatacc attgctcata gctgatggat
      781 cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaata
      841 cagcgacacc catgatcaat agcagtgcat tgactaaaaa agccattgac aactggatat
      901 tagagtccca agtagaaaag cgtaccgctt gtgctacatc ttcttcgtct tggtgatcga
      961 tcttccgagt ttgagaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac
     1021 caataatccc taacgctcca gtcagcggag tttcaccacc tatgacaggt gagtctgaga
     1081 atgtatgact gttagggata aagccagcaa ataaactgtg ccaatcaggg ttagacaagc
     1141 caacttgata gacgaaaacg aataaaatga cgaaaatcaa gcagacaacc aaagcttcga
     1201 ttttacgaaa cccgactttt gtcagcaata aaaggacgaa aacatctaac acagtgataa
     1261 atacggcaat taccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatga
     1321 cttctgcgat gtctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccca
     1381 atgtcttact tgtccgtgct cggattgctt gggccaaatc catttgggtc acgattccta
     1441 attttgctgc catatactgt aaaagcatag caattagact agacatcaaa ataactgaca
     1501 tcaataaata ttggaaattt tgtccaccag tgatcgaagt ggaccagtta cctggatcca
     1561 tataaccaac tgctactaat gcacctggtc cagagtaagc aagaagtgtt ttaaaaaaac
     1621 tttttccctt tggtacatcc actgttccat ttacttcagc taaagataaa ccatttgtat
     1681 gttcaataag tcgatgctta tgaaattgtt tgttggattg attattcaag aaaaaaccac
     1741 ctttcctatt tatctacgat tacattctac acctcagaaa gaaaaaatta aagaaaaaga
     1801 ttcggtatgc ctaatttttt ttcgtggcgt gtcttgctgg ttaatgaaag aaattttgtt
     1861 cttctctaaa aaaaccacta ttttttgcat gaaactaaag aatacgcctt tcttttttca
     1921 ataaacttta gtaaaataaa tcattgagag caattcggaa atagaaagga gagatttata
     1981 tggtatcaac aggtattgtc gtacttattg ctattattgc tttgctaatc ggcgcagtag
     2041 gtggcttttt cctagcgcgt aaatatatga aagactattt agaaaaaaat cctcccgtta
     2101 atgaggacat gttgcgatca atgatgatgt caatggggca aaaaccatct gagaagaaaa
     2161 ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa ataggttctg acccaaagac
     2221 tttttgcggg ctgatttttt cagtccgttt tttatttcgt aatttgttag actattcaga
     2281 ctattcagca aaaaagacaa ggacagacaa tcctatgctc ttgataaaga caaaggagga
     2341 gtaaaatgtc gatttttaga aaattaggtt ggttttttcg tcaggaaaag aaacattatt
     2401 taattgggat tttctcccta ttcgcagtgg ccttggtaca gttagttcct ccaaaagtga
     2461 ttgggatcat cattgatgag atcgccgata agaatttatc aataaagatc atttcttggt
     2521 ggatcttact attgatcctt gcagctttct tacaatatct ttttcgttat atctggcgga
     2581 cgaatatttg gggaagtgca gctcggttgg agaaagaact tcggcagcag ctattcaaac
     2641 attttacaga aatggacaat ctcttttacc aaaaatatcg gactggagat ttgatggctc
     2701 atgcaacgaa tgatttgaat gcaatccaga atgtagcagg agctggaatc ttgacctttg
     2761 cagattcagt gattacgggc ggaacaacga tcattgcgat gattttgttt attgattggc
     2821 gcttaacact gattgcgctg attcctctgc ctttgctagc cgttacttca cgagtgctgg
     2881 ggtcaaaact ccacgatgct tttcgtgatt cgcaagaagc tttttctagt atcaatgata
     2941 agacacaaga aagtatcact gggatcaaag tcattaaaac atttggacaa gaaaaagaag
     3001 atctcgcgga ttttgaagaa aagattgaag atgcaattat taaaaacaaa cgagtcaatt
     3061 ttttagactc cttatttgat ccattcatta ccttgattat cggtctgtct tatgttgcaa
     3121 caattatttt aggtggaaag tatgtcttgg aaggcacgat ctcgattggg cagatggtct
     3181 catttattag ttatatcggt atgcttgttt ggccgatgtt tgcgattggc cgattgttca
     3241 acgtattgga aagaggaaat gcttcttatg atcgcgttaa tgaacttttg catgaaaaga
     3301 cgcatatcgt ggaacaactg ggtgcagtgc agaccccggc aaaaggcgag ctgaatgtag
     3361 ctatttcctc ttttaactat ccgaaagaag aatcaccagc attagaacaa atcaaatttg
     3421 cgatccaaga aggagaaaca ttaggtattg ttgggaaaac tggtgcggga aaaacgacaa
     3481 tcatcaaatt actgatgcgt gaatacgatg attatcaagg gaaaatccaa tttggcggac
     3541 gttcgatcaa agaatattca ttgaatgctt tattaggatc aatcggttat gtgcctcagg
     3601 atcacttcct gttttcgatg accattcgag ataatatccg tttttctaat ccaaaacttt
     3661 cagaagaaga agtagagcat gcggcttctt tagcatttat caacaatgaa atcaaggaat
     3721 tgccaaaagg ttatgatacc ttagttggag aacgtggagt ctctttatct ggcggacaga
     3781 aacaaaggat ttcgattgct cgagcattga tcaaaaatcc tgaattgctg atcttagatg
     3841 atgcactttc tgcagtagat gccaaaacag aagaagctat cttgcataat ttaaaagaaa
     3901 taagaaaaga gaaaacaacc atcattgcag ctcaccgttt aagcagcgtg atgcatgcaa
     3961 aagaaatcat tgttgtcgat caagggaaaa tcattgaacg aggaacccac cacgagctgc
     4021 ttgcattagg tggctggtat gaacgaatgt gggaaaaaca gcaattagaa gcgaagatag
     4081 aaggaggcga ggcctgatgg atgaaaatta tcaatcggaa tggacaaaat cgattccact
     4141 taaagaacag aagaagatca tcaaacgact gatgaagttc gccaaacctt ttagaagaac
     4201 gttcatcgtt gctatcttat ttgcgtttgc cctgtctgtg atcaatgtcc tattgccaag
     4261 gatcatccaa acttttatgg atgatcatct ggctaaacag tcagctacga cacaagttat
     4321 tttgtttttt gctggtgtgt acttgtttgg agtcatagtc aaaagtatca tttggttttt
     4381 ccaatggtat ttgtattcca tggcttcatt gaagacctat cagtatgttc gggtaagatt
     4441 atttgaaaag ctgcatacac taggtatgcg ttattttgac cagacaccgg caggatcgac
     4501 cgtttctcga gtgacgaatg acacagaaac gctatttgaa ttttggtatg tctttttgat
     4561 ggtattgact gggatctttg cagtcgtttc ttctttcgtt gccatgtttc aaatcaatgg
     4621 agaaattgcc ttgtggactt tggtgttctt gccgatttta ggaatcgtca tctggtatta
     4681 ccagaaattc agttctcgta tttaccgaaa tatgcgggaa aaattaagtc agctgaacac
     4741 gaaattgaat gaatcgattt ccggcatgcg tattatccag cagtttcgcc aagaagctcg
     4801 tttagctaaa gaatttgaac aagtgaacaa cgagtattta gacacgcgct atgcgatgat
     4861 caaaacgaat tcgattctgt taagtccgat catcaatttt ctctatgcat tggcgattgc
     4921 gttggcgttg actttgtttg ggatcgatgc gttgcattct cccgtagaag ttggactgat
     4981 ctatgcattt acgacctatg tccagggatt ttttaatcca atgtctcaaa tgatggactt
     5041 tttgagcacc tttacagatg ggatggttgc cggtagtcgt attttgaaaa tcatggatac
     5101 agaagaatat gcaccgcaac aaaatcctgg agcagatcaa gcgattactt caggtaagat
     5161 cgagtttcgc aatgtaagct tttcttatga tggagagcac aatgtattga acaatatctc
     5221 ttttgtcgct aatccgggtg agaccgttgc attagtgggg cacaccggaa gcggaaaaag
     5281 ctcgatcatc aacgtattga tgcggtttta cgaattttat gaaggccaaa tcttgattga
     5341 tgatcatgac attcgagaat atccaatgga agagttacgt aagaaaatgg gattggtctt
     5401 gcaagatgcc ttcttgtttt atggagatat tgcaggaaat atccggatga tgaatccagc
     5461 tatcacagac gaacagattc gccatgcagc tgagtttgtt caagcaaaca agttcatcga
     5521 tgaattacca aaaggatatc actcaaaagt gatcgaacac ggtgccagct attctagtgg
     5581 acaacgacag ctgatcactt ttgcgcgaac aatcgtgaca gatcctaaaa tactagtatt
     5641 ggatgaagca acagcaaata tcgatactga aaccgaagga ttgatccaag aaggattggc
     5701 aaaaatgcga caaggacgga caacgattgc gattgcccat cggctgtcca cgattcggga
     5761 tgcagaattg attttagttt tagacaaagg tcatatagta gagcgtggga atcatgaaac
     5821 acttctagcg caaaatggtt tgtatgcaga tatgtacaaa ctgcaaagca gtggtgtgta
     5881 aaaagaggtt gtgacagtag catagtagca ttttgtccta agcgctagag tgaaaataaa
     5941 acagagcttc ccagattctt catctgtgag cgttagtggc gtagtcaccc gatttaaaaa
     6001 agacgacgac agctagtcaa aagtgcctaa tagccactaa gaaagcgttt gctttcgata
     6061 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
     6121 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgaa tacagcccta
     6181 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
     6241 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
     6301 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
     6361 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
     6421 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
     6481 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
     6541 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
     6601 ggaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
     6661 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caaaacaata
     6721 cctacctgtt tactataaca atacaggata ttctaaatat cgtccgctat tactataaga
     6781 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
     6841 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
     6901 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
     6961 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
     7021 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
     7081 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aatatattgg
     7141 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggtg
     7201 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
     7261 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
     7321 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
     7381 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
     7441 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcttagctg
     7501 ctggcgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
     7561 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
     7621 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
     7681 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
     7741 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
     7801 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
     7861 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
     7921 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
     7981 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
     8041 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
     8101 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
     8161 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
     8221 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
     8281 ttgtggggat tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta
     8341 tttttacttg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
     8401 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
     8461 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
     8521 cgctgacttt tttcttttgg aaggaggaag caggtgaaac agatgacaga cagaaaagat
     8581 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aaagcagcaa
     8641 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
     8701 gagaagtacg tgcccctttt gacggaacag tcatgaccct gttccccacg aaacatgcaa
     8761 tcggaatggt ttctgaaaca ggtctggaat tgctgattca tgtcggattg gatactgttc
     8821 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
     8881 atctgttgct ccgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac
     8941 ctgtaatcgt caccaattac atggattatt tggatatttt ggaaaatcaa ggggagacgg
     9001 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggaa
     9061 tagacttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
     9121 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
     9181 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
     9241 cgatgtattg atcgtaaaat taccaatttt tgaatacagt cgttcgcgtg tcgtaagtcg
     9301 caaagcggct gtactcattg aggatagcgt tgaatcaccg atactggtca acgctaaaat
     9361 tggaacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
     9421 ataagaaatc agaatcccgc acatatcact cgtgcaattg tacgcttcat agaacgattc
     9481 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
     9541 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
     9601 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
     9661 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
     9721 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
     9781 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
     9841 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
     9901 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
     9961 gatttttt
//
LOCUS       DAZKAO010000097         9873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000097 DAZKAO010000000
VERSION     DAZKAO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.098"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..267)
                     /locus_tag="ACG8H8_002101"
     CDS             complement(22..267)
                     /locus_tag="ACG8H8_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP8987156.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="ACG8H8_002102"
     CDS             complement(381..959)
                     /locus_tag="ACG8H8_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8987157.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="ACG8H8_002103"
     CDS             complement(1036..1566)
                     /locus_tag="ACG8H8_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP8987158.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="ACG8H8_002104"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="ACG8H8_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP8987159.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="ACG8H8_002105"
     CDS             complement(2423..2818)
                     /locus_tag="ACG8H8_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP8987160.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /gene="cysS"
                     /locus_tag="ACG8H8_002106"
     CDS             complement(2815..4227)
                     /gene="cysS"
                     /locus_tag="ACG8H8_002106"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP8987161.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="epsC"
                     /locus_tag="ACG8H8_002107"
     CDS             complement(4224..4760)
                     /gene="epsC"
                     /locus_tag="ACG8H8_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP8987162.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /gene="gltX"
                     /locus_tag="ACG8H8_002108"
     CDS             complement(5082..6542)
                     /gene="gltX"
                     /locus_tag="ACG8H8_002108"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP8987163.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="ACG8H8_002109"
     CDS             complement(6712..7869)
                     /locus_tag="ACG8H8_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP8987164.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="ACG8H8_002110"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="ACG8H8_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP8987165.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="ACG8H8_002111"
     CDS             complement(9336..9863)
                     /locus_tag="ACG8H8_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP8987166.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DAZKAO010000098         9831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000098 DAZKAO010000000
VERSION     DAZKAO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.099"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..1177)
                     /locus_tag="ACG8H8_002112"
     CDS             complement(194..1177)
                     /locus_tag="ACG8H8_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP8987167.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1182..1829)
                     /locus_tag="ACG8H8_002113"
     CDS             complement(1182..1829)
                     /locus_tag="ACG8H8_002113"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP8987168.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1914..2546)
                     /locus_tag="ACG8H8_002114"
     CDS             complement(1914..2546)
                     /locus_tag="ACG8H8_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8987169.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2562..3113)
                     /gene="coaC"
                     /locus_tag="ACG8H8_002115"
     CDS             complement(2562..3113)
                     /gene="coaC"
                     /locus_tag="ACG8H8_002115"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP8987170.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3110..3847)
                     /locus_tag="ACG8H8_002116"
     CDS             complement(3110..3847)
                     /locus_tag="ACG8H8_002116"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP8987171.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3844..4833)
                     /locus_tag="ACG8H8_002117"
     CDS             complement(3844..4833)
                     /locus_tag="ACG8H8_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP8987172.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5091..5633
                     /locus_tag="ACG8H8_002118"
     CDS             5091..5633
                     /locus_tag="ACG8H8_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP8987173.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5650..6735
                     /locus_tag="ACG8H8_002119"
     CDS             5650..6735
                     /locus_tag="ACG8H8_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987174.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6747..7553
                     /locus_tag="ACG8H8_002120"
     CDS             6747..7553
                     /locus_tag="ACG8H8_002120"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987175.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7554..8381
                     /locus_tag="ACG8H8_002121"
     CDS             7554..8381
                     /locus_tag="ACG8H8_002121"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987176.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8378..9472
                     /locus_tag="ACG8H8_002122"
     CDS             8378..9472
                     /locus_tag="ACG8H8_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8987177.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSLGYSLNSKNMAQLTEAANKLNHLTP
                     NVKAIVADEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMP
                     KTVKNKEGAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDT
                     ISHLEVYKDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2985 a         1808 c         2000 g         3038 t
ORIGIN      
        1 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
       61 ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
      121 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
      181 gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt
      241 ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc
      301 gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg
      361 ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa
      421 tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc
      481 aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat
      541 ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt
      601 gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt
      661 ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg
      721 ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc
      781 caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa
      841 catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg
      901 cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat
      961 cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt
     1021 tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt
     1081 tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat
     1141 ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat
     1201 tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa
     1261 atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt
     1321 tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc
     1381 aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca
     1441 taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca
     1501 taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt
     1561 ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa
     1621 atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca
     1681 aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc
     1741 cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt
     1801 ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc
     1861 attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta
     1921 gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga
     1981 cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa
     2041 acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc
     2101 atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa
     2161 acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc
     2221 accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct
     2281 gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg
     2341 ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct
     2401 aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata
     2461 gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt
     2521 aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta
     2581 agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa
     2641 tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat
     2701 gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca
     2761 atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca
     2821 atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca
     2881 gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct
     2941 gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg
     3001 cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca
     3061 gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt
     3121 cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt
     3181 ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca
     3241 aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca
     3301 gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct
     3361 gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct
     3421 cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat
     3481 tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa
     3541 aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt
     3601 cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa
     3661 tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg
     3721 tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat
     3781 gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa
     3841 tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag
     3901 tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc
     3961 aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac
     4021 agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag
     4081 tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag
     4141 aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc
     4201 taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag
     4261 actttttagc catgtgacag actcttcttt tctggaaaag gtaatgattg actggaatcc
     4321 tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac
     4381 aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt
     4441 aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac
     4501 taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag
     4561 cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac
     4621 gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga
     4681 ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac
     4741 ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc
     4801 ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc
     4861 ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa
     4921 ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc
     4981 agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt
     5041 ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg
     5101 gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac
     5161 gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa
     5221 tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc
     5281 aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg
     5341 aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc
     5401 ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg
     5461 aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg
     5521 tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc
     5581 atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg
     5641 ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat
     5701 gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt
     5761 ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct
     5821 acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga
     5881 caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac
     5941 gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa
     6001 gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct
     6061 ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg
     6121 ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa
     6181 ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa
     6241 gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt
     6301 ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg
     6361 gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag
     6421 caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc
     6481 ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact
     6541 cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg
     6601 atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca
     6661 gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac
     6721 agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt
     6781 atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt
     6841 tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga
     6901 catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc
     6961 tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc
     7021 tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg
     7081 ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga
     7141 aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat
     7201 ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta
     7261 gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat
     7321 taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc
     7381 tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt
     7441 tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta
     7501 tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa
     7561 aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct
     7621 tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta
     7681 cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata
     7741 cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta
     7801 tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca
     7861 tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac
     7921 taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa
     7981 tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg
     8041 ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt
     8101 ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg
     8161 ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg
     8221 caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca
     8281 tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga
     8341 aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt
     8401 tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac
     8461 aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc
     8521 tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga
     8581 ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat
     8641 accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca
     8701 ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc
     8761 agataatcag tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa
     8821 attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga
     8881 cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg
     8941 ctattctttg aatagtttag gctattcttt gaatagtaag aatatggcac agctgacaga
     9001 agcagcaaat aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat
     9061 caaaatgtat atgatcaatg aagaagcctc cgtggcagtg acattttctg gcgaagctgc
     9121 cgatatgatg tgggaaaatg aacatttaca ttatgtgatc ccaccagaag gatccaatct
     9181 ttggtttgat aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt
     9241 catcaacttt atgctagaac cggaaaatgc ggcacaaaat gcagagtata ttggttattc
     9301 tacaccaaat gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg acgaacaatt
     9361 ctatccatct gatgacacga tctcccatct tgaagtctac aaagatctag gatcgaaata
     9421 cctaggcatc tataatgacc tattcttaga attcaagatg tataggagat aatggcaaaa
     9481 ctcagtaaga atggggactt atcaacgttc taaagagcta aaaatcgtcc tttttctatc
     9541 tgagttttcc aatcttctac caaaagcgga tgaaacgccg ctttttttgt gttattttat
     9601 tttttctacc acgtttttct accagagaaa agtggacaaa accattgtaa aatagggccc
     9661 gagacgggct agtaaatcta agattgaaaa agttacagaa ggataaagta attggaaact
     9721 atgcaaatat acttaaccct tctgtaatct attaataaaa ctaatatatg aatccagttc
     9781 aatatttgta gctatgaaaa aacataaaaa tcatattaga taaaaactaa a
//
LOCUS       DAZKAO010000099         9808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000099 DAZKAO010000000
VERSION     DAZKAO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.100"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..680
                     /locus_tag="ACG8H8_002123"
     CDS             201..680
                     /locus_tag="ACG8H8_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP8987178.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="ACG8H8_002124"
     CDS             713..2941
                     /locus_tag="ACG8H8_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8987179.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="ACG8H8_002125"
     CDS             3070..4713
                     /locus_tag="ACG8H8_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP8987180.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="ACG8H8_002126"
     CDS             complement(4786..5331)
                     /locus_tag="ACG8H8_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP8987181.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="ACG8H8_002127"
     CDS             complement(5354..5839)
                     /locus_tag="ACG8H8_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8987182.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="ACG8H8_002128"
     CDS             complement(5829..6641)
                     /locus_tag="ACG8H8_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP8987183.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="ACG8H8_002129"
     CDS             complement(6780..8312)
                     /locus_tag="ACG8H8_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP8987184.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="ACG8H8_002130"
     CDS             complement(8421..9452)
                     /locus_tag="ACG8H8_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987185.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3258 a         1827 c         1892 g         2831 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcagcgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggata
//
LOCUS       DAZKAO010000100         9665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000100 DAZKAO010000000
VERSION     DAZKAO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.101"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..630)
                     /locus_tag="ACG8H8_002131"
     CDS             complement(<1..630)
                     /locus_tag="ACG8H8_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8987186.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            973..1389
                     /locus_tag="ACG8H8_002132"
     CDS             973..1389
                     /locus_tag="ACG8H8_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8987187.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1386..1856
                     /locus_tag="ACG8H8_002133"
     CDS             1386..1856
                     /locus_tag="ACG8H8_002133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8987188.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1870..2643
                     /locus_tag="ACG8H8_002134"
     CDS             1870..2643
                     /locus_tag="ACG8H8_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8987189.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2657..3469
                     /locus_tag="ACG8H8_002135"
     CDS             2657..3469
                     /locus_tag="ACG8H8_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8987190.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3523..4488
                     /locus_tag="ACG8H8_002136"
     CDS             3523..4488
                     /locus_tag="ACG8H8_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8987191.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4604..7327
                     /locus_tag="ACG8H8_002137"
     CDS             4604..7327
                     /locus_tag="ACG8H8_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8987192.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7340..7972
                     /locus_tag="ACG8H8_002138"
     CDS             7340..7972
                     /locus_tag="ACG8H8_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8987193.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8871..>9665
                     /locus_tag="ACG8H8_002139"
     CDS             8871..>9665
                     /locus_tag="ACG8H8_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987194.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1475 c         1884 g         3045 t
ORIGIN      
        1 aatcatatgg aagccaccga ttaggtattt tatgggttta gtcggaaatg tttcaagagc
       61 aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa tcagaccgtc
      121 ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa atttatcatc
      181 catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga tgatatggaa
      241 gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca ttttaaggat
      301 ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat aatggggtaa
      361 agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc ttaacccgcc
      421 ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca aagtttcccc
      481 cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga gatatttctt
      541 ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca aatttattct
      601 tgagttttca aataaaacct tgatttccac tataacatct cctttcacat gaacgtgtat
      661 tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa aaatattaat
      721 ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg tggcggggga
      781 ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt taaataccca
      841 cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat agaaaaacct
      901 ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag cgtaaggata
      961 ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc tcaaggatta
     1021 aagcaaacag ctacaatgat tattggagat agtgtaccaa ttcatgcctt gtcagcattt
     1081 agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac aatagatata
     1141 gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga tatgctcact
     1201 attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc tttagttatt
     1261 tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct tattaaagag
     1321 agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac tggaggagat
     1381 gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag gtagtatttt
     1441 cttggagtaa atcattaaat atcaatcgta ttattattgc caataaagag gcagcgaatg
     1501 atgaattcaa aaaaatgtct ttgaatttat ctaaaccgac tggcattaaa ttaacaattt
     1561 tttcagttga acagttacta gaaaaaatgc ctaaaatcga aacacttaac gaaaatatca
     1621 tgcttatttt tggtaataca aaagaaattc gtcaattttg tgaagcctat cctaaagtac
     1681 aagaaatcaa ctatggaggg ataatcaaaa aagaaggttc caagcaattt agtaatgcca
     1741 ttttcttgaa tgaatctgag caagaagatg ccaaaaaatt aaaagctctt ggcattaaac
     1801 aatttattca acaggtacca acttctaaaa aagaagattt gaatacaatg ttataaagga
     1861 ggaatgaaaa tgctcgttca gtcaattggt ttagggttaa tcggtgtttt ttgtatttta
     1921 gactcaaggc ttttaggaag aatgaatttt gaacgaccgt tgattgtcag tacgcttgtt
     1981 ggtttggtac ttggagacat ggaaaaagga ttaattgttg gagcctcttt ggaattgatg
     2041 tccttaggat tagtaaatat cggagctgct gcaccacctg atatgaatat ggcatcaatt
     2101 attgctacag cctttgccat tttatcaaat gcgaatgcag aagcagcatt aacgattgcc
     2161 attccaattt cagtgttagg tcaaatacta ggtatcttaa tgcggacgat tttgtcaaat
     2221 ttgactcacg ttgcggatcg ctttatcgaa caagggaaat ttagccaagc aaggagaatt
     2281 catatacttt atggctcggg attatatgct ctcatgtatt ttattcctat ttttctagca
     2341 atttattttg gaacgaatct tgtttctcag attgttgatg cgattcctga gtggctaaca
     2401 aatggcttaa cggttgcaag taaaatttta ccagcatatg ggtttgcgtt gttattacaa
     2461 acgatgttaa cgaaaaaaat ggtgccattt ttattacttg gatttttaat tactgcttat
     2521 tctggattgg gaatcattgg tattgcagcc ttcgcatgta ttgttgcgtt tgtcatggtt
     2581 caagttcaag gtaaaaactc aaatggtaac caaaatgagc cagatccatt agatgactta
     2641 taaggaggta gattaagtga aagagaaaga tcaaaatgca tggaaatatc ggcagttctt
     2701 atggcgttca tgggcaattc aagcctcatg gaactatgaa cgacaaatga acatgggttt
     2761 tatgtacgga attgcaccaa ttctagataa catttacaag aatccagaag atgaagcaat
     2821 gaagaaagaa gcttacgagc ggcatatgat gtactacaac tgtacccctc aaacaagtgc
     2881 atttgtgtta gggttaactg cttctatgga ggagcaatac tacgaagacc aagaaaactt
     2941 taatccggaa tcaatcagtg cagtgaagac ttctctgatg ggaccgctat ctggtgttgg
     3001 tgattctttc tttcaaggaa cgattcgggt attagccttt ggactaggaa ttaatttagc
     3061 ccaacaaggt agtattttag gtccaattct agcaatgtta atttcctttg taccttcctt
     3121 tttagttact tactatggtg ggaaaattgg ctataacatg gggaataaat acttgtctaa
     3181 actgtatcaa gaaggtttga tggataaagt aatgtatgtt tgctccattg tggggttgat
     3241 ggtcattggt tcgatgattg ccagcatgat cgggattaca acaccaattc aaatgggtaa
     3301 agcttttgtc ttacaagatg tattagatag tattatgcca caatttttac cattaggatt
     3361 aaccttcttc atgtactggt tattgcagaa aaaggtcaaa acaggatgga tgttggcaat
     3421 ctgtttagtc ggaggaatta tttttagttt gttaggtatc ttacaataag ggaataacca
     3481 ataaatttgg ttgaataata taaataagga ggaattcaat tgatgaattc aaaagaaatt
     3541 gttaaagaaa tcgtagcgaa aaaagaggta aaatcaatcg tatttgttgg ctgtggcgct
     3601 tcaaaagcgg acctttatcc tgcaaagtat tttctagacc aaaatgcgaa gcagttacgg
     3661 attagtcatt acacagccag cgaatttaac tatgcaacac cagcatctgt agatgagtca
     3721 actgtcgtta tttcagcttc tttaggaggt acgactccag aaacagttga agctaacgca
     3781 gtagctaaag aaaaaggtgc tacagttatt agtctaacac gaagcattga ctcaccgtta
     3841 acaaaagatg cagattatgt aatttatcat cgatttgcag agaactatgc tgctaaatta
     3901 gaaaaaacag gctactgttt agaattggca gtagaacttt tacatcaagt agaaggttat
     3961 gaccattatg aagcaatgca agaaggattt gctactatct atgatcttgc ccaaaaaagt
     4021 gcagaatcag ctcgtaaaga tgcgaagaaa tttgctgaac gctttaaaga tgaaaaagtt
     4081 atttatgtaa tgagtagtgg tgctacacat gaagtagcat actcaacatc aatttgcttg
     4141 atgatggaaa tgcaatggat taattctggt accttccatt caggagaatt tttccacgga
     4201 ccattcgaaa ttgtggataa agacgtaccg tttatattat taatgaatga tggaaaaact
     4261 agaaatatag atgctcgagc attaactttc cttgaacgct ttgatacgaa aacagaagtt
     4321 gtagatgcct tggattatgg cttgtcttca catattgcta aagaagtctt agactacttt
     4381 aacccattcg tgattactgc agtatttaga gtttatgcag aagaattgtc agaagcaaga
     4441 gaacatccat taactaagag acgttatatg tggaaactaa cttattaaaa aatagatcaa
     4501 actaaataag gtggcttgag aaattaagcc accttgtttt cttcaatgtg attttataca
     4561 tatggtaaaa taactagtac atattgtgaa agcaggtggc aagatgcagc gattaaatga
     4621 ctatctttat caactaacat tgcttttgaa atctacgcct tcagataatt attcagctcg
     4681 cagtatttcg caagctttac agttgaatag aagtacaatc agtagctact taaatgaagg
     4741 tgttcgtgaa gggcttttca taaaagtaaa aagttacccg gttttatttt tacatagaac
     4801 agctcttgaa gaactacata tcacactgaa taactccgaa atagaatcaa ttgaaagctt
     4861 gttaacaatt tctcaaaaac ccgcattaga tcaagtaatt ggctctaaag gaagtttgaa
     4921 agaagcaatt gatcaaatta aaacggcagt cttatatcct ggaaaagggt tgcctttatt
     4981 attgatcggc gctagtggat cagggaaaac ctttctagcg aataaaatat atgaatatgc
     5041 agtagaagaa aaggtcattc acacatcggc acctttcata gattataatt gtgcacaata
     5101 tgtgagcaat ccagagttac tatcctctgc tttatttggc tacacgaaag gagcatttac
     5161 cggagcaagc caggagcata ctggtttgct agaaaaagcc gatggaggag tattgttttt
     5221 agatgaagtg catcgcttat ctgaagaagg gcaagagaaa ttatttacat ttatggatac
     5281 gggtgaattc tctcctatgg gggataatag tattcggaaa aaagccgatg ttcgattagt
     5341 ctttgcaaca acagaaaata tatatacgac tttcttacca acttttctta ggagattgcc
     5401 agttattgtt aatctaccta gatttcaaca acgtccgtcg tttgaacgtc tgtcattgat
     5461 tgatgaattt tttgtttctg agagtaaaat tttagctaag gaactatctg tttctgatgc
     5521 attgattcat ttcttaatga atagtgattt tgaaggtaac gtggggaata ttaaaaatat
     5581 tatcaagtat gcttgcggaa acgcatatat ccaccagaag agtcagcaaa caattcaagt
     5641 taaattattg gatatgcctt tagagtacag ttataaattc aaggagcaac taaataaacc
     5701 cagaaagaaa agagcagatc gaatctacta tccagaagaa acacgacaag tgcaactaga
     5761 aagaaacaat cagctttttg ttcaattttt tgaccaatta cttactggat tctctgaagt
     5821 gattgaaaat aacaaggata ttaaactatt tttagaagaa atggttacca aggtaacaca
     5881 agtaatggat acgcttattt ttcaagagga ttacgaaaaa gagcagtctt tatacgccat
     5941 tctaaactat catattcgtc aaagtattga ttttatgcga gaaagctacg ggtttgaaca
     6001 agatggtaac cgggtagtcg ctttggccaa ctatctatat gtcaaggata atcaagtgat
     6061 ttttgaaaat agtcttcaag aaaagaaaac caaagatcag cttaacgaat tttttgctaa
     6121 tgttcttgaa accccttatt ggtatgcaaa aaaaatctta agccttttat ctgagtgttt
     6181 agatcaacct gtgtatgaag aggacatttt ctttattacg ctatattttt atagtttgaa
     6241 tgtcacgaat gttaaaacag gtattaagag tattgtgcta gctcatggtt attctacagc
     6301 tagtagtatg gcgaatgttg ccaaccgtat gctgaataag aacgtttttc aagcttatga
     6361 tatgccttta gatattacat tagagaaaat tgaagcaaaa ttgattcaat tccttggaga
     6421 ttatcgaaca gattcaggct tgattttatt ggtagatatg ggttcactaa accaactagg
     6481 cggtttatta gtgaatcatc taaatggacc actgttaatc attgatcata tcaatacacc
     6541 gttattattg gaagtcggcc aacaagtatt aaacaataaa agtatcaccg aaataggagc
     6601 agaaattgaa acaagtctta ctttcaaaaa gcagttacta cttcccaaga aaaagaaaaa
     6661 gaaagccatc attacttgtt gttatacagg gattggtagt gcgacccaga ttcaggagat
     6721 tttaacgaaa tgcttgaaag atgcagttag agaattagta attattccgt atgattacaa
     6781 aaagctgtta gaaaacaaga attttgagac tccttttcaa ttatacgatg tcatcatgat
     6841 tgtaggtaca gaagatccca aaattaatca gattccatat attggattag atcgcctgat
     6901 taatggggaa gcaattggcg aatttgttca ttacttggaa aaagagctga ctattgaagt
     6961 gaacgattta caaaaagaat taattttcaa tttttcaatt agtaaaattg tcgaaaattt
     7021 aaccattttg gatccagata aggtgctatc ttttgtacaa aaagcaatta aagaattatc
     7081 gaagttatta aattttgatt tttctaataa tcagctgttt ttgttgtacc ttcactgtag
     7141 ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat gagcaagagg acattgaaga
     7201 atatatgcaa gcaaatggag aaataatgga taaaattcat agcgcttttt ttgagattga
     7261 aagggaatac acaattgaaa taccagattt agagttgcga ttattgagcg atattgttca
     7321 aggataggag tgaattcgaa tgacaatggg agtaattttt gatatggatg gggtactcat
     7381 agacagcgag acattttatt ttgagagaag aatgaacttc tttcaagaac tggagataga
     7441 tccaggatca aaccagattg atgattatgt ggggaaaaca gaagctgcaa tatgggaaac
     7501 attagttcct gaggacaaat cattaagaga ggaactttat ccgaaatatg tagattatcg
     7561 aaaaacgcat ccaattgatt tcgctaaaga tttacgaaaa gaagtgaaac cgttacttga
     7621 cttcctcaag aagcgggata taccattggc actggcatct tcttctccag ttgttgagat
     7681 agagagtatg ctcattcaat gtgaacttac tgaatatttt tcctttgtta taagtggtga
     7741 gtcattaaaa caaagcaaac cacatcccga aatatattta aaagcgaaac agcaattaga
     7801 ctgtgacttc tttattgcag tagaggattc aacgattggt attaaatcag caaaggcagc
     7861 aaatatctat acggtggcac taaaacaacc aatgcaaatc gatcagagtg aagcagacat
     7921 acaaattgaa agtctacaag aactggaaca aattgtttta gaaaaaggtt agtgaattaa
     7981 tggaaataag gctatatatc tgtgctagct attttgacta accaaaaaaa cttgctataa
     8041 ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa aatgcttcgg cagtgataat
     8101 gggattggct ttttaagttt tttattttgt tcgcaatccc taaggtcatg tgaatgatcc
     8161 tagggattgc tagaatattt tgcaacattc tatctatttc agaacgtaac cttccccagt
     8221 tcattgggat tcattagcat acaaccaaca tttcaaaaag aaattgcact acaccattat
     8281 tagctatttt gactaacccc aaaaaacttg ctataattca ggtgtaggta aaaggtgctg
     8341 gtatcacctt ttacgaaact gcttcggcag tggtaatgag tgtggcttaa aggactagtc
     8401 ctttaagctt tttactttgt tcgcaatctc taagatcaag ttaatgatcg ataagattgc
     8461 tagaatattt tgcaacattc tatttcagaa cgtaaccttt cccattccac tgtgattcat
     8521 tggcaaccct cctcacaacc aacgtttcaa aagaaattgt aaagttgttg gggtaccacg
     8581 accgaacaat actacacctg aatgatagta agttgtctca ttaagtatag catatcctct
     8641 ttttgggtaa atagaaatcg gttacatttt ttatccaaga ccaagttttt tcatttggtc
     8701 ttatttgcta tacccaaaca attccgcagt atagtcgacg gtattgattt tatcaggtga
     8761 gtaatagaaa cttgttttcc ttccccaaaa cattacctat aaaggagaaa aaatggtatc
     8821 gtaattatat aggtatagga tgaaattttt ttctaagagg agggttgttg atgaaaaatt
     8881 tacagttaaa atttattgtt gatccatcga ttgtacgctg gtttcggatt atcaatcaat
     8941 ttgagcgcaa tcaacttgcc acaaaaggag aattagcaaa gagtaatcat gtatctgaac
     9001 gaaccattca aacagatgtc aataaaatga aggactactt ttctgaaagt gttcaatttg
     9061 tttcaacgaa aagtgggtat tcttttcaga aggaaagacc ggttcttttt ctcaagcaga
     9121 aggagcaact actggagaac gaaattttat ttgagttgtt agggaatgtc ttttatggcg
     9181 aattggaaga tatgacggag ctaattgatc gttttcattt atctgaagcg acttttcgtc
     9241 ggtatttgaa gcagattcaa ccagttttgg aagaatacgg cttagaattg agtctttatc
     9301 ctcttgattt aaaaggagaa gaggcaaata ttcgcaagtt ttttaaagac ttctattatg
     9361 aaggggagat gacgccccat actcttgttc caccaaggga tcttcatgaa cttgtgcaag
     9421 ctacattcta tgataaattt gagtatttct cgattggaac agggacttct cctgctgaat
     9481 tttattatag tttgtatatt gcgattgagc gtgtgagaca aggaaaaacg atagccattc
     9541 ctcaaggtct gattgagcga gtaattgcta gtgagaattt tcgcttaatg tattccttga
     9601 aagaagcgat tcaaacaaat tatcaagttg ctttatcaga agaggagttt tgttggttgt
     9661 attta
//
LOCUS       DAZKAO010000101         9642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000101 DAZKAO010000000
VERSION     DAZKAO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.102"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8H8_002140"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8H8_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP8987195.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG8H8_002141"
     CDS             1995..4661
                     /locus_tag="ACG8H8_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8987196.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG8H8_002142"
     CDS             5025..5777
                     /locus_tag="ACG8H8_002142"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8987197.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG8H8_002143"
     CDS             complement(5799..6953)
                     /locus_tag="ACG8H8_002143"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP8987198.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG8H8_002144"
     CDS             7138..9579
                     /locus_tag="ACG8H8_002144"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP8987199.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1736 c         2067 g         2728 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tg
//
LOCUS       DAZKAO010000102         9570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000102 DAZKAO010000000
VERSION     DAZKAO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.103"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8H8_002145"
     CDS             <1..818
                     /locus_tag="ACG8H8_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8987200.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="ACG8H8_002146"
     CDS             938..1138
                     /locus_tag="ACG8H8_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP8987201.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="ACG8H8_002147"
     CDS             1214..1411
                     /locus_tag="ACG8H8_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987202.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="ACG8H8_002148"
     CDS             complement(1708..3255)
                     /locus_tag="ACG8H8_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP8987203.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="ACG8H8_002149"
     CDS             complement(3339..4142)
                     /locus_tag="ACG8H8_002149"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8987204.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8H8_002150"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8H8_002150"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP8987205.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8H8_002151"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8H8_002151"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP8987206.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8H8_002152"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8H8_002152"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP8987207.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="ACG8H8_002153"
     CDS             complement(6527..7456)
                     /locus_tag="ACG8H8_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8987208.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="ACG8H8_002154"
     CDS             7651..8334
                     /locus_tag="ACG8H8_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFP8987209.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="ACG8H8_002155"
     CDS             8495..9538
                     /locus_tag="ACG8H8_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8987210.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAZKAO010000103         9480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000103 DAZKAO010000000
VERSION     DAZKAO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.104"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8H8_002156"
     CDS             168..362
                     /locus_tag="ACG8H8_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987211.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8H8_002157"
     CDS             631..930
                     /locus_tag="ACG8H8_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987212.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8H8_002158"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8H8_002158"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987213.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8H8_002159"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8H8_002159"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP8987214.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8H8_002160"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8H8_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP8987215.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8H8_002161"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8H8_002161"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP8987216.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8H8_002162"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8H8_002162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8987217.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8H8_002163"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8H8_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP8987218.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8H8_002164"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8H8_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8987219.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8H8_002165"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8H8_002165"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP8987220.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8H8_002166"
     CDS             7521..8291
                     /locus_tag="ACG8H8_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8987221.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8H8_002167"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8H8_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987222.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2955 a         1938 c         1485 g         3102 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
//
LOCUS       DAZKAO010000104         9463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000104 DAZKAO010000000
VERSION     DAZKAO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.105"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1347
                     /gene="lysA"
                     /locus_tag="ACG8H8_002168"
     CDS             88..1347
                     /gene="lysA"
                     /locus_tag="ACG8H8_002168"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP8987223.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1334..2134
                     /gene="dapF"
                     /locus_tag="ACG8H8_002169"
     CDS             1334..2134
                     /gene="dapF"
                     /locus_tag="ACG8H8_002169"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP8987224.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMTSGEDAIQMAGPAVHEWTRILEE"
     gene            2137..3018
                     /gene="dapA"
                     /locus_tag="ACG8H8_002170"
     CDS             2137..3018
                     /gene="dapA"
                     /locus_tag="ACG8H8_002170"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP8987225.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3015..3731
                     /gene="dapB"
                     /locus_tag="ACG8H8_002171"
     CDS             3015..3731
                     /gene="dapB"
                     /locus_tag="ACG8H8_002171"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP8987226.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3737..4432
                     /gene="dapD"
                     /locus_tag="ACG8H8_002172"
     CDS             3737..4432
                     /gene="dapD"
                     /locus_tag="ACG8H8_002172"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP8987227.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4448..5449
                     /locus_tag="ACG8H8_002173"
     CDS             4448..5449
                     /locus_tag="ACG8H8_002173"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP8987228.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5446..6807
                     /locus_tag="ACG8H8_002174"
     CDS             5446..6807
                     /locus_tag="ACG8H8_002174"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP8987229.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6767..7918
                     /locus_tag="ACG8H8_002175"
     CDS             6767..7918
                     /locus_tag="ACG8H8_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8987230.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7893..9062
                     /locus_tag="ACG8H8_002176"
     CDS             7893..9062
                     /locus_tag="ACG8H8_002176"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8987231.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9237..>9463
                     /locus_tag="ACG8H8_002177"
     CDS             9237..>9463
                     /locus_tag="ACG8H8_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8987232.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         3107 a         1653 c         2138 g         2565 t
ORIGIN      
        1 tgtttaatat tattccaata aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg
       61 aaaaaaagtg aaaagaggaa tgaaaaaatg acagaattga taattggcgg agtcacagca
      121 agcacattgg cagaaaagta taagacacct ctgtatgtgt atgatgaaga aaagatgaga
      181 caaacgatgc tgtcattcaa gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt
      241 tacgcctcta aagcattcca aacgattgag atgctgcatt tagttcgtga atatggattc
      301 gggctagatg ttgtcagtgg tggggaaatc tataccgctt tgcaagctga ttttcctaga
      361 gatactatct acttccatgg taacaacaag acgccagagg aactgatcta tgcacttgaa
      421 aatgatttgt tgcatattgt tgccgataat ttgatggaag ttgaattatt agcagaatta
      481 agccaacgct atttaaaaaa aatctgtatc atgcttcggt tgaatgtcgg agtagaagca
      541 catactcatg aatatatcgt cactgcgcat attgattcaa aattcggcat gagctatgaa
      601 agtaaagagt gccaacaagc ccttcagttg ataaaagaaa gcagctatct agagttagaa
      661 ggatttcatg ctcatatcgg gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc
      721 gacaaattga cgaattactt aaccgatttt gaccaacctc tttccttgaa tcttggcgga
      781 gggtttggga ttcgttatac acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa
      841 aaactgattg aatatacgga agaagctttg gaaaaacgaa aactgaccat tcgtcaatta
      901 ctgatcgaac cgggtagaag tattgttgga gaagctggta caacactata cacgattggt
      961 ttcatcaaaa agacaccaca taaaaaatat tactttgtag atggcgggat gacagataat
     1021 atccgtccag ctctgtatca ggcagaatac gattgtgatt tagcagcaaa actgtcggaa
     1081 gaaaaaacag agaaagtgac gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt
     1141 aaagaagctt atttgccgaa agcagaatcg ggtgatcttt tagctgttta ttccacggga
     1201 gcttatggat actcgatgag cagcaactac aatcgagcga cacgaccagc agttgttttt
     1261 gtcaataatg gccaatcaag actgattgtt cgcagacaag cattttctga tctactaagg
     1321 ggggaagttt cctatgatcc ttcataaata taatggctgc ggaaatgatt ttatcttatt
     1381 agattacgca gaagacacag attatagtca attagccgct tctttatgca caaaagaaaa
     1441 atatgatata gacggattga tcgccgtaaa aatcgatccg ttggaaatga tctattacaa
     1501 caaagatggc agccgcgcgc cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt
     1561 aaatgatcag aacatgatga atgaacagga attcaacgta gagacattag caggcacaag
     1621 aaaaattacg gtgttggatc cctcaccttt ttattgcagc gtagatatgg gagtgccaga
     1681 tgattctccg gaaaaaatca aagctaatca aacttatcca ataaaaaacc aggaaataga
     1741 gatcaatggt gaaaaagtaa cgatcactag tttattttta ggaacgattc ataccgttgt
     1801 ttttgttgaa aatgcgcgtg aggaagtctg gagaaaaaga ggtgaactga tctgtcatca
     1861 tcctttgttc aaagaaaaaa ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt
     1921 gatcgtccgt acctatgaaa gaggagtagg ttggacactt gcttgcggta caggttgctg
     1981 tgcctcttat gtggttgcta aggaaactgg gaaaatttca gcagatcagg tagttgttca
     2041 cttggaacaa ggagacttga tgacctctgg tgaggatgcg atacaaatgg ctggcccagc
     2101 agttcatgaa tggacaagaa tattggagga atgaagatga taaaaggttc gatcgtggcg
     2161 ctgatcacgc cgatgaatga agagggatca gtagattatg ctggtttgga aaaattgatc
     2221 caattccatt tagatgaaca aacagatgga ttattagttt taggtacaac aggagaatct
     2281 tctacgctca cacaatctga ggaggagcag atccttcaac tgacagtaaa gaaagtggct
     2341 ggccgtgtgc cggtgattgc aggagctgga acgaataata cgaaagaaac gattgaaaaa
     2401 gcgaagcatt ttgcttcttt gggggcagac gcacttttgg tcatcacccc ttactacaac
     2461 aaaacaagtg atgcaggact agctgctcat tttacagcaa ttgcagaagc ctcaccgatt
     2521 ccgcttattt tgtataatgt tccttctaga acaggcatgt cgattccaat ccatgtatta
     2581 gtaaatttag cagaacatcc taatatcatt ggtttgaaag aagcctcagg agatatggct
     2641 tatgtgatgg atgccgctcg gttgattgga gaagaattct tcctttacag tggaaatgat
     2701 gatttgatcc taccagtaat gagtgtagga ggaagcggtg taataagtgt atgggcgaat
     2761 atccaaccta aaatagtcca tgaattagta aaagatacgc aggacggaag atggcagcaa
     2821 gcaaaagaaa agcaattaaa tgcacttgaa ctgatccatg ccttgttcag tgagaccaat
     2881 cctattcctg tcaaagcagc catgtcatta ttagacttac cttccggacc tctacgcctg
     2941 cctttagttt ctctttcaaa agagaaaaag aaacaattag ctcagctttt gttaaaagaa
     3001 aggacggttg aaaaatgaaa atcggactga ttggcagtgg acagatggga aatagagtcg
     3061 aagaagttgc acaagaaaga ggagatacgg tccttctttt acggacggca cctaaagaag
     3121 caactacgct tgcttttacg gaactgccag aactgcttat tgatttttct catccagata
     3181 atttagagaa aatcctttcg tatagcttat catatcaact tccgttagtc attggcacga
     3241 caggttatac acaatcacag ttccaagaaa taaaagaaca tgccaaacat gtacctgtga
     3301 tgtatagtgc taatttttcg ttcggcatca tggtgatgaa tcaactaatc aagcaagcag
     3361 ctgcaatgtt gcaaggctgg cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag
     3421 atgcaccatc tggtactgca aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc
     3481 agcccgtata caactggcaa gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta
     3541 ttcgtgcagg ttctttacct ggtgaacatg atgtattgtt tgctgctact gatgaaatat
     3601 ttacgatcaa acatgaatct ttttctaatc gaatctttgc tgcaggcgca gtagaagcag
     3661 cggactggtt gaaagatcag tcaccaggtt attacaaatt agaagattta ttaatggaca
     3721 aaggggtgta aaaaacgtgg aagctcaaga aatcatccgc tacattcaaa cggcatctaa
     3781 aaagacgcct gtgaaagtct atctgaatgt aaccgagcca atcgaattta aagaaagtaa
     3841 agtttttggt gaaggaagca gcttcgttgt ttttggtgac tatgagacga ttgctccggt
     3901 tttagaagct gaatcagaaa aaatcattga aatcgaaatc gaaactgctg caagatacag
     3961 cgcagttcct ttgctagata ttaaaaaaat taatgctcgt atcgaaccag gtgctattat
     4021 cagagatcaa gtgtcaatcg gcaacaatgc cgtgatcatg atgggagcga ttatcaatat
     4081 tggtgcagta atcggtgaaa acacgatgat tgatatgggt gctgtcttag gtggacgagc
     4141 gacagtgggc aaaaactgcc atattggtgc aggagctgtc ttagcaggtg tgatcgaacc
     4201 agcatcggcc aaaccggtta tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat
     4261 tgttgaaggt gtccatatcg gaaaagatgc agtcgtggca gcaggagcag tggttttaga
     4321 agatgtggca gcagaaactg ttgtcggtgg aatacctgca cgtgtattaa aaataagtga
     4381 tgatcagaca aaagaaaata cagcattgat tgctgcgtta cgcgaattat agaggagatg
     4441 gagaaaaatg cgcgaaatag atacctgtat tgtgggggca accggattag tcggccaaac
     4501 gatgctcaaa gtgttagaag aatatgattt tccagtagga cgattgaaat tattagcttc
     4561 aaaacgttca gccggtaaag tttgtgtatt caagaaaaag aaacacactg tacaggaatt
     4621 gacagaaact tcatttgaag gatatgattt agcgctattt tctgcaggtg cttctgtatc
     4681 caaagagttt gcgccaatcg cacgaagtca gggactattt gtcatagata attcctcagc
     4741 atggcgggaa gattcggata ttccattaat cgttccagag gtcaatctgg aagactatac
     4801 attgaatcgg ctgattgcaa atccaaattg ttcaaccatt caagcggtac ttccgcttaa
     4861 agctttacaa gaagcctttg gtatccagcg agtcaattat tcgacctatc aagcagtttc
     4921 tggttccggg caaaaaggca ttgaagatct tcatcgtacg caaaatgacg aggaaccagt
     4981 tttgtatcct tataatatta gccaaacggt gattccggaa atcgatcagc ctttggaaaa
     5041 tgggtataca aaagaagaac agaaaatgat aaatgagacc cgtaagattc tgcatcagcc
     5101 tgctttgcca gtatcagcaa cctgcgtacg tgtgccaatc gaaaatggac atggagtctc
     5161 tgtggcagtt caactgcaaa aagagtttac gacggaagaa attagagagt gtctcagcca
     5221 gtttccaggg attcaattag aagatgatct agcagcacat aaatatccga cgagcattct
     5281 tgctagagga acagatatgg tttatgttgg gaggatcaga aaagacactt ctttagagaa
     5341 cggattgttg ttctatacga ctgccgataa catacgcaaa ggagccgctg cgaatgccgt
     5401 acagattgct gcagctttaa tgcagaaaat agcggaggtg gcaagatgag taatacgctg
     5461 aaagtagcga aattcggcgg aacatcgatg tctcatgaag agcaattcaa aaaagtccgt
     5521 acaattgttc tttcagatcc caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt
     5581 acgtcagatg atgacaaagt aacagactta ctttatttga tttatgctta ccttcagcat
     5641 catgttgatt ggacacccct ttggaaaagg atttgtgatc gtttcattca agtaagagat
     5701 gttcttgccc tgcagttacc tattgaagat gaactagcac gcatacagaa acaactggaa
     5761 gacgaaaaga taactgaaga ttatttagta agtagaggtg aatatctgac tgctcaactg
     5821 atggctgagt atcttggtta ccagtttatt gacgcaaaag acctgatttt ctttgactat
     5881 gatgggaaaa tcgatcagga aaaaacagca caggaaatca caaaagcatt taaaagatat
     5941 ccgaaaatgg ttgttccagg gttttacggg gcgaaccctg caggaaaaga aaaattattg
     6001 ggccgtgggg gaagcgacat taccggcgcg atactggcga agatgttgca agcggaaagt
     6061 tatgagaatt ggacggatgt ttcaggaatc atgatggctg atccgcgaat catcgatcat
     6121 cctaaaatca tcgaggaaat ctcttttaga gaactgagag agatggctta tatgggggct
     6181 aacgttcttc atgaagcagc tgttttccca gttcaagagg ctgatatccc catccaaata
     6241 aaaaatacca atgatccttt cgctcaaggg acaaaaatat cgaatcacga aatagaaaag
     6301 gaaaatggac tgacaggaat agctggacgc aaggattttc tttccatcac tttattcaaa
     6361 cgtcatatgt ccgatgaaat cgggtttatc tggaaagcaa tgagcatttt tgctaaacat
     6421 gagatatcaa tcgaacatat tccttcaggg atcgacaata tcggagtgat cgtctcagca
     6481 gaagcgattg ccgataagtt atttctcatc acgaaagaat taaaagaaac acttggcgtg
     6541 gaagaaatcg tcgtgatcga agacttagct ttgatctctg tcgttggggg tccgcataaa
     6601 gaattgattg gattatctgg gaaagtgtta tcgatattga acaaattaga aattcgcaca
     6661 tcgatcttat cacaaggagc acaggagttg aatctgatca tcggtgtacc gaataatcaa
     6721 tatgaaacag tagtgaaggg tatctatgaa gggatggtga atacggatgc tgaaagaata
     6781 tcgaatggca ctccatcaaa ttcctgaaat cgggtttcat gaattcaaga caaaaaaata
     6841 tttatatgaa caagtaaaaa acagcgaggc cgttatccat gaaatcggcg aaacaggact
     6901 actcctttat tttgataatc agcaagaaga aacgattgct tttcgaacag atatcgatgc
     6961 actgccagtc aaagaagaaa cgaatctctc ttttgcttct aagcatgaag gttatatgca
     7021 tgcatgtggt catgacggac atatggcgat gcttttaggg ttgactgact ttataactaa
     7081 acacattgag gatcttattt ataatgtagt actgatctat cagccatcag aagaaatttc
     7141 cggtggcgca caaagcgtta ttcagtcagg attgttggat aaatatgaag tacaagcaat
     7201 atttggcttt catctatggc ctggactagc taagggagaa gttttctctc gaccaggacc
     7261 tttgatggca caaagcagtg aaacagacat tatcattaaa ggccagtctg cacatattgc
     7321 ttctagtgaa aaaggaatcg atagtctgga ggcagctgta cgatttttga agaatgttta
     7381 tgattttgac cggcaattac cggcagagtg gaatcatcta atgaaatttg gccaaatcca
     7441 aggtggaacg atccgaaatg ttttagctgg agaagttatt gtatccggaa gtatccgttc
     7501 ttacagttca gatatgcaaa attatttgaa gaggggatta gaccatttag cagaacagtt
     7561 tcactctaca gctactattt cttttcgcta taatgacggc tatccagctg taatcaacca
     7621 tagaggctta tatgcagcac tgttagattc aaacaaacta catgaattag atgctccagt
     7681 tcttcaagcc gaagattttg gcatatatac gcaacaatat ccatgtgtat ttttcttttt
     7741 aggtgtcggt gatacccctg ctttacatga tccgacgttc gattttgata tggatgtttt
     7801 agaaaatggt gtagaatggt acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat
     7861 cgatagcagt tcaaaagagg agcgagtaaa taatgatgaa tttatccaat cgatttagtc
     7921 ctaggctttc tagaatagaa gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga
     7981 taccagatgt gatcaaactc accctgggag aaccggattt tccaactcct gaacatgtca
     8041 agcaagcagg aatagcagcg attgaagaag acttttctca ctataccgga atgagaggat
     8101 tagaggaact gcgggaagcg gcttgtatat ttcagcaaca gcgttatgga ttaacttatg
     8161 atccgcaaac ggaagttttg acgacagttg gtgcaacgga agcgattgcc tctgcgttgt
     8221 tatctgtttt ggaagaagga gacaaggttc ttattccagc gcctgcttat tcaggatatc
     8281 agccattagt ggaactagct ggtgctgaat tgatcccgat agatacttct gacacagggt
     8341 ttgtctgtca gccggaacaa tttgaacgag catttgaaca atatggatca gctgtcaaag
     8401 cagtcatttt aaactatcct aacaatccta ctgggacgac attgtctgca aaacaattga
     8461 aagcaatcgc tgaggtttta aaaaaatatc cggtatttgt tataagtgat gaagtatatg
     8521 ctgaattgac ttatagtgga acgcatatgt ccatagctac ttatcttcca gaacaaacaa
     8581 tcgtgataag cgggttatcc aagtcacatg cgatgactgg ttggcgagtg ggctttattt
     8641 ttgctcaaaa agcccttatt gacgaactaa ttaaagtcca tcaatatctg gtgactgccg
     8701 ctacaacgat gtcgcaaaaa gctgcggctg aagcgctaat aaatggggtg aaagatagcg
     8761 agaatatgaa agagcaatat caaacacgaa gagactatct ggtccagcaa ttggcgcctt
     8821 taggatttga agtgacccag ccaaacggtg cattttactt gttttgcaaa ctcccagctg
     8881 ccattcaaac tgactcttgg cagttttgtg tagaattagc ggaacaggca aaagttgcgt
     8941 gtatcccagg aattgcattt ggcccagaag gagaaggtta tatccggatt tcttatgcta
     9001 gcagcatgga aaaattacat gaggcctgca aacggatcaa agacttttta acagaacagt
     9061 gattcataga ttttatcaaa aaaacaatcg ttaattttgc ggtatattct gcaaggagac
     9121 gattgttttt taaatttagt tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat
     9181 gttaagttta aattgtttag aatgatttta aaagaatatt caaggaggtt tcatcaatgg
     9241 aaaaaatcaa agttgtcatt gtaggtgcat ctcatggagg gcatcaatcg atcttggaac
     9301 tattgacacg atacggtgaa aatgtagata tcacgctatt tgaagcagga gactatattt
     9361 ccttcatgtc atgtggtatg gagctgtatt tagaagacaa ggtaacagat gtaaatgatg
     9421 tacgtaattt cagaccagaa aactttccgc aatccaatgt tca
//
LOCUS       DAZKAO010000105         9269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000105 DAZKAO010000000
VERSION     DAZKAO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.106"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1487
                     /locus_tag="ACG8H8_002178"
     CDS             <1..1487
                     /locus_tag="ACG8H8_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8987233.1"
                     /translation="VKYATQIEDGMFDPNGFIEDMKPFFADRERKYLAYTSEKNEIDN
                     RPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVSEQKRTLIQRLKDKFL
                     KNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAFRLLEPVEEDDGWKLR
                     ASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKILTYACYGDLNFVTSGT
                     FKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRALSDQQNEIEQFKLAYM
                     LVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFNEYHMDLLKKQFYTIC
                     KAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKECAETIAAFLKFHDKV
                     EIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIPFVTDVDYENEMMELE
                     SGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSDT"
     gene            1405..2457
                     /locus_tag="ACG8H8_002179"
     CDS             1405..2457
                     /locus_tag="ACG8H8_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFP8987234.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            2459..2776
                     /locus_tag="ACG8H8_002180"
     CDS             2459..2776
                     /locus_tag="ACG8H8_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987235.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            2903..3526
                     /locus_tag="ACG8H8_002181"
     CDS             2903..3526
                     /locus_tag="ACG8H8_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFP8987236.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            3541..4431
                     /locus_tag="ACG8H8_002182"
     CDS             3541..4431
                     /locus_tag="ACG8H8_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFP8987237.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            4454..4669
                     /locus_tag="ACG8H8_002183"
     CDS             4454..4669
                     /locus_tag="ACG8H8_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987238.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            4681..5022
                     /locus_tag="ACG8H8_002184"
     CDS             4681..5022
                     /locus_tag="ACG8H8_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987239.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            5022..5393
                     /locus_tag="ACG8H8_002185"
     CDS             5022..5393
                     /locus_tag="ACG8H8_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987240.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            5386..5781
                     /locus_tag="ACG8H8_002186"
     CDS             5386..5781
                     /locus_tag="ACG8H8_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFP8987241.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            5783..6160
                     /locus_tag="ACG8H8_002187"
     CDS             5783..6160
                     /locus_tag="ACG8H8_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFP8987242.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            6161..6769
                     /locus_tag="ACG8H8_002188"
     CDS             6161..6769
                     /locus_tag="ACG8H8_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP8987243.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            6769..7110
                     /locus_tag="ACG8H8_002189"
     CDS             6769..7110
                     /locus_tag="ACG8H8_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987244.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            7352..>9269
                     /locus_tag="ACG8H8_002190"
     CDS             7352..>9269
                     /locus_tag="ACG8H8_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP8987245.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEV"
BASE COUNT         3402 a         1246 c         1866 g         2755 t
ORIGIN      
        1 gtgttaaata tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaag
       61 atatgaaacc attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga
      121 atgaaataga taatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc
      181 atgctggtat gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta
      241 tcaaatggga ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata
      301 aattcttaaa aaatgatata gaacttccga cagttccaga agcttttaac aaattaacaa
      361 gcaatcttga ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag
      421 cttgtggagt agctttccga ttgctagagc ctgtggaaga agatgatggt tggaagttaa
      481 gagcaagtaa tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca
      541 aagaaaagta tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa
      601 ataaaattct gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca
      661 aaaaaattga tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata
      721 atacgaatcg ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg
      781 ctttgtctga tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg
      841 gcacaacaat gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact
      901 taaaagcccc tactgcaaaa gctgaatatg tcacaaaaaa tttagcaaaa gatttcaatg
      961 aatatcatat ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta
     1021 acgatgaggt gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc
     1081 tagaagcgaa aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag
     1141 aaacaattgc tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta
     1201 tttttacttt ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta
     1261 aacttgcacc atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg
     1321 ttgattatga aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat
     1381 acaattttgg cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga
     1441 gaactggagg atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt
     1501 gtatttcctg aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac
     1561 aatatcactt atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt
     1621 gaatatgtgg aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt
     1681 atcgatgaat tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga
     1741 tcagatgtat ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt
     1801 tctgatcgct tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt
     1861 caacttttaa acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg
     1921 aactatccat ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt
     1981 gaacagaaac tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac
     2041 gcattaaaca ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca
     2101 aagtataaca aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat
     2161 ggtattgaaa aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa
     2221 gatgagagag tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa
     2281 gatgctgtca taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt
     2341 cctaatttac caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat
     2401 ttattagatg agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat
     2461 gaaagatttt tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta
     2521 caaaacagct attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa
     2581 ttatgcctac gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga
     2641 taagaatacc gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga
     2701 aacagttgat tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg
     2761 aaagtctagt aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact
     2821 gggcttggtt gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa
     2881 cgaatcgtgg ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt
     2941 gcaatacttt gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt
     3001 ggaatccaat gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa
     3061 acaattggaa gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga
     3121 atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc
     3181 tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga
     3241 acaaattgct ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct
     3301 aaataatctt gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag
     3361 tgttttgaat gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc
     3421 tgcatttggt ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac
     3481 aaatccagaa gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt
     3541 atgaaaaaaa catctttaaa taatttagag tatttggata tttcaccagc gattaatgct
     3601 atgcaagtac caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca
     3661 gcaaactcga cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa
     3721 aaacttgagg gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat
     3781 actgaaattt ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat
     3841 ggagtcggaa atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat
     3901 ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga
     3961 atgaatggga ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt
     4021 gcagtaacaa gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg
     4081 ggagaaaaac tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat
     4141 ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga
     4201 aatatcgtgt ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga
     4261 acaattgccg caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca
     4321 gaagaacttg caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt
     4381 gaatacaatg cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt
     4441 agtactccct tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga
     4501 tgaagtcgtg aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa
     4561 aactcgtaga tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa
     4621 agaagcaatt gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg
     4681 atggatgaat cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg
     4741 agtgaagaac gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac
     4801 acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat
     4861 aaaataataa aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg
     4921 attagttatg cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg
     4981 atacgaagag ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt
     5041 tagaaactac ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta
     5101 atacaaatat tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc
     5161 tttcacaagg tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt
     5221 caactgacac atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa
     5281 taacaataac tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt
     5341 actatcctag tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa
     5401 tatgaatcaa atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa
     5461 aaagaaggaa tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca
     5521 ttaactcctg taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa
     5581 ctatcaagcg gtcgaattgt tggagaagtt ataaacaatg aacattattt taaatatgta
     5641 gaagaaggtc ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt
     5701 gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa
     5761 atcgttaaga aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt
     5821 aaaagaaatc tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga
     5881 tttttatttc gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca
     5941 aaatgtttct ttcttaattg atattgcatt gattgataat aaccctaata gaaatttaat
     6001 taatgaatta gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat
     6061 attatttcca aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac
     6121 attagaattt ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa
     6181 aaccggaatt atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa
     6241 ttttccggta tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc
     6301 taattatgaa acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt
     6361 tggtggtgca gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat
     6421 tactggaggg aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga
     6481 tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta
     6541 taaaggacag tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag
     6601 ttttactcat ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta
     6661 ttcagttgat gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa
     6721 agtagcaatt ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat
     6781 caaacaacga ttaaattaac gaaaaaagac gaagaaggta aatatgagca agtacaattt
     6841 aaatctgctg aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa
     6901 atgcaaacag cgactgataa gcaatcagtt aaaaaagctt taagtcgtgc ttattcattt
     6961 attgcagata ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat
     7021 gctcgtgaga ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat
     7081 gaaacttata cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt
     7141 attcgattac ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt
     7201 taccagaaat tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg
     7261 cggtaaaaga gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga
     7321 tactttgaat aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc
     7381 cggtatgaat aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag
     7441 tagaaatagt ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc
     7501 tagcggagta actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg
     7561 agcggttgat cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt
     7621 tagtaattca gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca
     7681 aggacttcct actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac
     7741 aggtgattta actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt
     7801 cggtggttca actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa
     7861 cggaaaagta gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct
     7921 ttctgctatt gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca
     7981 aggtaagatt tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg
     8041 cggaggactt aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc
     8101 tatacaaaat gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa
     8161 taaagcatta gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag
     8221 ttcaatggaa tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt
     8281 aagtaattta tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt
     8341 tcttgttcct gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc
     8401 aataataaaa agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa
     8461 gattatgggg gcaatagctg ctgctaatcc atttgtttta attattggtg cagtcgttgg
     8521 tgcaatagct gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat
     8581 atctatttgg aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg
     8641 gggaattgat acttggaatt ctgctaaaga aatggcatca aatgcagttg agggtgtcaa
     8701 agatgcttgg tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg
     8761 ggccacaggt ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa
     8821 aaatgcatgg tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc
     8881 agccagagaa aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat
     8941 tacaggtata aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa
     9001 tttagttgag attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt
     9061 tttgtttatt acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc
     9121 tgtttggaat aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt
     9181 tgtaagttat tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct
     9241 tacattgata aatatttgga atgaagtgg
//
LOCUS       DAZKAO010000106         9235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000106 DAZKAO010000000
VERSION     DAZKAO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.107"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1458
                     /locus_tag="ACG8H8_002191"
     CDS             4..1458
                     /locus_tag="ACG8H8_002191"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP8987246.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1552..2694
                     /locus_tag="ACG8H8_002192"
     CDS             1552..2694
                     /locus_tag="ACG8H8_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFP8987247.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="ACG8H8_002193"
     CDS             2807..3814
                     /locus_tag="ACG8H8_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8987248.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="ACG8H8_002194"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="ACG8H8_002194"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFP8987249.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="ACG8H8_002195"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="ACG8H8_002195"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFP8987250.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="ACG8H8_002196"
     CDS             5793..7388
                     /locus_tag="ACG8H8_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP8987251.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="ACG8H8_002197"
     CDS             7404..7718
                     /locus_tag="ACG8H8_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP8987252.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8179..8904
                     /locus_tag="ACG8H8_002198"
     CDS             8179..8904
                     /locus_tag="ACG8H8_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8987253.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9037..>9235
                     /locus_tag="ACG8H8_002199"
     CDS             9037..>9235
                     /locus_tag="ACG8H8_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP8987254.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINF"
BASE COUNT         2820 a         1665 c         2156 g         2594 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
     8161 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
     8221 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
     8281 ccaaatggtg ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag
     8341 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
     8401 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
     8461 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
     8521 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
     8581 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
     8641 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
     8701 acgttaaaag aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc
     8761 aaggtaaaga agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga
     8821 agtgtagaat ctgtatttaa tgaagagaat ttaaaaaaag cctatggaga tactatattt
     8881 attggagagg ggggaacggc ttagcctctc aatccaataa atatattatc tcaaaagaaa
     8941 tgagttaata ccaaattaaa ataacgatgg ttcatagggt tatccaatca aaaccggaag
     9001 gttaaaaccc taactctaat atacaagggg aataatatgc ttcaaaattt tattaatggt
     9061 ctgtatgact ttcacttttt acagaatgct ctaattacat ctgtagttat agggattgtt
     9121 tctggaacag taggttgttt tattatttta agaggaatgt ctcttatggg tgatgctatt
     9181 tctcatgctg ttttgccagg agtagcaatt tcttatattt taggtattaa ttttt
//
LOCUS       DAZKAO010000107         9072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000107 DAZKAO010000000
VERSION     DAZKAO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.108"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(282..1400)
                     /locus_tag="ACG8H8_002200"
     CDS             complement(282..1400)
                     /locus_tag="ACG8H8_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987255.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1633..1755
                     /locus_tag="ACG8H8_002201"
     CDS             1633..1755
                     /locus_tag="ACG8H8_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987256.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1871..3178
                     /locus_tag="ACG8H8_002202"
     CDS             1871..3178
                     /locus_tag="ACG8H8_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP8987257.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3299..4498
                     /locus_tag="ACG8H8_002203"
     CDS             3299..4498
                     /locus_tag="ACG8H8_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP8987258.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4525..5793)
                     /locus_tag="ACG8H8_002204"
     CDS             complement(4525..5793)
                     /locus_tag="ACG8H8_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP8987259.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLFYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPILQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(5849..5915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5957..6604)
                     /locus_tag="ACG8H8_002205"
     CDS             complement(5957..6604)
                     /locus_tag="ACG8H8_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFP8987260.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(7013..7516)
                     /locus_tag="ACG8H8_002206"
     CDS             complement(7013..7516)
                     /locus_tag="ACG8H8_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8987261.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7639..8403)
                     /locus_tag="ACG8H8_002207"
     CDS             complement(7639..8403)
                     /locus_tag="ACG8H8_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP8987262.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8511..8786
                     /locus_tag="ACG8H8_002208"
     CDS             8511..8786
                     /locus_tag="ACG8H8_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP8987263.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2719 a         1934 c         1614 g         2805 t
ORIGIN      
        1 cttatttctt gaagctgacc acttctgtca caacctcttc cttaaacggt gttcaaaagc
       61 ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga agagctattt ttgcttgctg
      121 ttcttattac ttgatgcaga acagcttttt cacaacctct catacacggt gtttaatcag
      181 ctgaccttcg gtattagctc aaaaaacggc aaaatatgtg aaactatttt tccgtttgtt
      241 tctctaatac tcaggtcaaa acactgattt cacaacctct tttaataacg aagaatcgct
      301 gtcagactct tttcatctgg cgcatctttt tgatccaaaa taaacacttt tccgccatta
      361 atgatcacat catctgctaa tgcattcaac acttgacgcc gatcatattc tgtttcagga
      421 tcttcaccga aaccatcagc taagtttgac gtagcgataa ataaatgtga cacttttcct
      481 tctttcgcag ctggaacgat atcaactaac tgatcgatga atttcttatc taagagttta
      541 ttgtattctt ttgattcttg agctgcgagt tcacttgaca gcttcttcgc cccttcttcg
      601 atatctctaa aggatagctg cgcaggagaa gcagaaatcg caacgtctgc acaataatag
      661 ggatttttag caattttttt gaacatcgtc tgattttctg gtagagcaaa taaataaatc
      721 ggccattgtt ctggattttc gaaagaatcc ttcaagtacg tatcaactgc ttggtaatag
      781 ttgacccaat cgatctctac ttcttcatct tttggattta cgccatggaa agttactcca
      841 tctcgggctc cgcctcctcc attagaagag aagttcaaac cgccacctgt cagttcagat
      901 cccaatgcag taacgacatc aaccggtgcc ccttctggta gagatacttc ttttacttgt
      961 ttgttgctga cttcatacaa tttcatggag tcgcggttca atcccaataa ataataagaa
     1021 tagttgaact gcccattttt gataatagcc agtaaataag gaagatcact tacatagtat
     1081 tgatcgtcta ccgggatact caaacgatgg atataggttt ggtcagcagt tataatgacc
     1141 gctacactcg tagttgcact acgccaaaac gaaggatctc ctaacaaatt ttcaattttt
     1201 tcttggaacg gggaccagtc atgttcggaa tattttttct caaaacgttt ctttgcttct
     1261 ttcgcaaaat ttttgaattt gatctgatct ttttccacat cttgatgggc gacatgcgta
     1321 ttcagcatga tcgtgacgaa tggtccttta gcgccttctg aagtcaattg tgacaataat
     1381 tctttatcct tttttgccat aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat
     1441 ctaaagtcta ccacaacact atttttccat acaataaaac gtatctaaaa aaataaaacg
     1501 gtttctacgg ttttaaattt agctattttt ttaagttatc ttcataattt cctcaattat
     1561 ttagccccag tcttcaacta ttcttcatga aatgctgata aactaagaaa aagaataaaa
     1621 gaggtggatc ttatgcattt gattatgatt ttcagtttat taagtattat cagtttattt
     1681 gctttcagcg cttcccgctt attagatgcg gaaacacagc ctattcctgt aaaagtacgt
     1741 aaaaacgacc gctaaccttc cataacgtgc gttcttatcc taaagttttt tcagaaacat
     1801 tgaaactctc tcccctctct gataaaattt taatttgtag cgaaacaaat taattacaag
     1861 gagatcggat atgtccataa aaaaaacacg tctagtccta ttattggcta gtctcattct
     1921 ttgccttgct tcttttctac cagctgttgc tgtgcaagct gaagatactt tcaaagtaaa
     1981 tgcaaaagca gcctttgctg tagatgcgca aagcggtaag attctttatg atcaagatgg
     2041 agaaaaaacc atggggatcg cttcaatcac aaaaattatc ggtctttaca tcgtccttga
     2101 tcaagtaaaa gaagggaaac tatcatggga tgataaagtt tcaatttcag attatgcaga
     2161 aaatttgagt gtcaccccag atttatcaaa tgttcctttg cataaagaaa acacctacac
     2221 cgttaaagaa ttattcgact ctgcaatcat ccagtcagcc aacgcttcaa tggttgcctt
     2281 ggcagaaaaa atctctggca gcgaagcaaa attcactgaa cgaatgaaag aacaattgaa
     2341 agattggggt atcaaagatg caacgatcgt taatgcatca ggactgaata actcttatct
     2401 tggcgaaaac cgaccagaag gtactggaga aaacgacgaa aatcaaatgt ctgctcaaga
     2461 tgtggctatc gttgcccgtc atttgatctt ggattttcca gaaatactgg atgtatctag
     2521 cactacgaca caaatgtttg gcgaaaacac gcaatcacca gtagaaatgg tcaactggaa
     2581 ctggatgctc ccaggattcg tgaattataa agaaggcgtg gacggattaa aaactggtac
     2641 gactgaactt gcaggagcct gtttcgtcgg tacgatcact aaaaacgatc aacgaatcat
     2701 cactgtcgta ttgaatgcag acggccatgc ggaaaaccca agtgcccgtt tcaatgaaac
     2761 agctcgctta atggattatt gctacgacaa ttggtcagaa aaagagcttg gaaaagccaa
     2821 tgcaagtatc cctagtctaa aaaccattga tgtaaaagac ggaaaagaat caacagtgcc
     2881 cgttgtgctt aaaagtcctg taaaagtatg ggtacggaac gatatggata ctggaaaatt
     2941 aacaatcaca ccaactcttg acaaaaaaca agtcactgac aatgagatcc aagcacctat
     3001 ggataaagga acaaaaatcg gtaccgcaac gatcaccttg gcagaagata aattaggtta
     3061 tctagaagac aacaacgcgc catccacaga aatcatcacg gataaaaccg tagaaaaagc
     3121 aaatattttc gttattggct ggcgacgcgt cgcagaattt ttcggtaact tgttttaatt
     3181 gaacaaaaag aaaaaagcat ctttccatgt ggaagatgtt tttttgcttt ttctagttta
     3241 aataacagct tttttcatct attttcgtta tactgtataa gaaaaaggag ttgaatttat
     3301 gaatctaacc agagatctga tttttttggt tctctatctt attggggtat tctccatcgt
     3361 ttattggcga aaacagaaaa aggatccttc cttgttcttt tacagcagta cgtggacatt
     3421 tgcggtaatc ttgacttatt ttgtcgtgtt ggaaattttt tattcgacct tttatttttt
     3481 gattccctta gtgtttttca gtatctttgc cttttcctat ttttcagaaa agcgaaggct
     3541 gctgaatggt cttttattca atgttttcct cattagtttt ggtatctact tgtttgtctt
     3601 gctatacgaa acacaaaata tttttcttgg cggactgatt gccttgatca cgatcccgtt
     3661 actgcttgtc tttctattcg gcatttatgg attgatcgtt tttctttttt ggaacggtgt
     3721 cacagtttta cgtagagagt cgcattcatt agctaatctt ttaacgttga tactagccat
     3781 ttttcttaca ttgtttcttg tttttgactt tttcttgtta aaatatttac cacaatggat
     3841 aaatgctttg tttttctgca tcccattgat cttgatctat ctatttatcg tattctacaa
     3901 ctttttgact gtctcattct tgtatcagtt caaccgccct cgatacaacc aagacttcat
     3961 cgttgtgtta ggcgccgggt tgatcaatgg agaaacagtt tctcctcttc ttgccaagcg
     4021 aatcaataag gcgattgctt tttattgggc acaaagccgt gcgacattaa atccgcctat
     4081 cctattaatg tcgggtggac agggagcaga cgaaaaagta ccagaagcaa tggcgatgaa
     4141 acaatatgcc atggaacaag gaattcctga aagagatatt cttgtcgaaa ccaattcgac
     4201 aaccactttg gagaatatgc gctattcaaa ggaaatcatg gatcagcaaa tgaaagggcc
     4261 ctatcgcgcg atcttcagtt ccaacaatta ccacattttc agagctggtc tctatgcacg
     4321 acaagcgaaa ctgaaggcaa atggcattgg ggcaaaaaca gctttctatt acttgcctaa
     4381 cgcgttttta agagaataca tcgccatctt gatgatgaac aaaaaaagac atatgatcat
     4441 ttgcggattg cttttcgcag gttccgcatt cttatcactg atcactttac ttttctaaaa
     4501 aaccaaggtg tgacaaaaca tatgtcacac cttgtttttt tcttttattc tcttgacgat
     4561 tggcaagaat agtattcctt ttttgacggc tacttgctgt gcagaataaa taccgctgtt
     4621 tcctgaacac ataaagctga tcaagcagat cataaagaag taaacagcag cttgtgaacc
     4681 aaataattcg attcccatca caaaacaggc aatcggtgta tttgtcgcac cagaaaatac
     4741 cccgataaaa cctaatccag caagaaaagg aacagaaaca tggagcaaag gcgctagtac
     4801 tgcgccaagc gttgcaccaa tctcaaatag tggtgtcact tctccccctt ggtaccccgc
     4861 acctaaagac aagaccgtga agaataattt cccaataaaa tcataagcat gtgcctgtcc
     4921 attaaatgat tcctgcagta tcggaagact caacccaagg taacgttgtg tttgcaaaat
     4981 caacgctgcg gctacaacta ctgctccacc tatcgtattt cgccaaacag gatgaggaat
     5041 ccattgcgca taccgcgctt ttaaaaagac gatcgaacgg ctgaataccc aaccaatcaa
     5101 accaaaagca atcccagcaa caaaaacttt gaagaacagc aacatactcc attcaggaat
     5161 cacacccatt tggtaatgta gatgagaaac accatagctt tctgtcatga aatttgcaaa
     5221 aagcgcagca aagaagctag gaaagattgc ttctgttcgg actttaccga ttgccaacac
     5281 ttctaatcca aataatgttc cagctaaagg tgtgccaaat acagaactaa aacccgcact
     5341 gattccactg ataaccaaaa tctcccgttc tgctttatcc aatcggaata aatgcgctat
     5401 attatcagct aatgctcccc ccatttgaac tgctgttcct tcacgaccaa cagatccccc
     5461 aaataaatgg gtagtgatgg tcccaaacaa ggtcaatgga atgagccgca aagggatctt
     5521 ctcttctcct ccattccctt gatcgatcac taagttattc ccacgactag aaagaccgcc
     5581 atgatacgca taaagataag caaaaagcgc gccgctgact ggcaatagat aaaatagcca
     5641 aggatgtgct agtcggatat ctgtcacgaa cgtcaggcta tttaagaaaa aagcagacaa
     5701 gctccccatg aatactccga gaatcgctgt gaccaccagc cacttcaaca tgtacatcaa
     5761 aatacgcact ggactcggta attgtttttc cataaaaaat tcctcctcaa ataaaaaaac
     5821 gcctactaaa gaaaggggct ggccccagac tttagtaggc gtcattagct atcttacgtt
     5881 agcatttaaa ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct
     5941 gtaaaaaaca ataaaattat cgttctttct ttacacgttc agcaaattca tagaaaggag
     6001 ccaatttcgt taactgttga ttacattgat cactatcatg acaaatataa ttcccctttt
     6061 tcgtatacgt gccatcagaa ccggctttcg ttgtagaaag aaagagcgag acattcgatg
     6121 ttttctgaca gatcgcacag attcccttat taatcgttgg tgataaagta ccgctgatcc
     6181 cttttaattt tccatcttcg taataaacca tgaatttttt ttgcgtccct ggatcattcc
     6241 aaccgatgta cgtatgtgct tgcaagtcga tttcattcca agcgggttgt ttgagttttt
     6301 tcacttttcg gaataatttc tcgatttgtg gtttgctcgg ctgcataaac ggctggacga
     6361 tttgcttcag ctcctccaat gctcgttctg cttgcgtttt tgtcagtttt acatctaaaa
     6421 ctgtcgttaa aaacgtatcg atttctgatg gttcgtaacc taatacatcg cgaatctttt
     6481 cagctgctag agcctgaacc gtttgtaccg tttttgagtc attgacagat gagtacgcat
     6541 ttaataactg ctctacttct ctttttataa aaaaatattg atatggtttc attgtttgtt
     6601 ccatgatttt aaagtccttt cttctaacca aagtgcatac tttgtagaaa aaagtgatct
     6661 tctgcaaagt atgtgttacc tgaagatccc ggactctttt aaataagata cgttttgcat
     6721 cttatcatca ccccctttaa tgttcattct tctatgtaca tgattcattc gataaataaa
     6781 agtaaaccgt atcttgagca gatagtcaac tgcaaacaga aaaaggactg tgacaaaagt
     6841 cgtgtcaaag tccctttcta caaacaaacg gtgttccaga agtagcttct tcaaaaaata
     6901 agccggaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     6961 gctgacccac ttctgtcaca acctcaatat ggtctattat tggttttacc tgttaaatcg
     7021 aggattcttt tccgaagtaa cgttgccatc tcttacgcat acttgctgac ataggctgag
     7081 cggcttcttt gactgcgcag tatttgtcta cgaatgttac gatatatcct tctttgtact
     7141 taggcggtgc aatcgttgcg ccccacatgt gcttcaaaat gatatctcgc tcaagatccg
     7201 acaaatcggt gatcttttct gcattcttta cagcgattct tggatggata tacgcatgtg
     7261 tgccttcatc aaatttcgtc gtacgccaat catagtagaa cagatcatgc aaaagtccag
     7321 cacgcgctgt tgctttagca ttgccacccc atttttttgc gagcagataa ctatagtaag
     7381 aaacactgat ggaatgctcc aaacgagtgg aatgaacatg ctgtgtgtaa ttcgctaatt
     7441 ttttcactgc ttctgtttct aaaagatctt ctacataaga catatattct tcgtcttcac
     7501 gccatttttc tgtcataatt atcgatctgc cacctttttg aatttcattt tcttctgaaa
     7561 ttgatctaag tataccacca gtgttcggat tattctagtc gccttggata aaatagccaa
     7621 agcttaatag ttccttaatc ataaagaaaa atgataaaga acaatccctg ccgcaacccc
     7681 aacattcaac gattccgcat ttcctttcat cgggatatat acgttctgat cagtcaaaga
     7741 caagatttcc tgacttaccc cctgaccttc gttccccatg attattgcga agttatctga
     7801 tttttctact ttgtctaatg cgtgtgcttc ttcatttaat tctgtgccat acactgtaaa
     7861 ccctttgtct ttcattaaag gaatcacttc tgtcaaatct ttttgcaaca caggcaaatg
     7921 ataattgctt ccctgcatgc tgcgcagcac ctttgtacta taaatatctg ccgttccttt
     7981 gcctaataaa acaccagcca tatcaaatgc atctgccgta cggatcatcg tccctacatt
     8041 tccaggatct tgtacattat ctaataacag ccatcctcct gaaaaatctg gcgtgccttc
     8101 agccgttaac gccatcactg ctaagattcc ttgtggtgta ggtagatccg atacagcatc
     8161 catgacctca tccgaaacta gaaaagtaga aatctctttg tttgcggcca gccagtctcc
     8221 ccattctttc atgcctcgct gtgtcacaaa aagttctttt atttctgcat cagacgacgc
     8281 agcttcttcg atcagatgga acccttccaa aagatattct tccttttctt cccgatattt
     8341 ctttttatgt agttttttta attctttgat tcttggattt tttgctgatt ggatctcttt
     8401 cacattcctc acctccccat gattatatca tggaataggt gcttgagctt gtttcatcct
     8461 aaaaaaatcg gtatcattaa tttataaaat aggaaagaaa ggtgacgaac atgcgcaaag
     8521 ttaaaatgaa cgtccaagga cgcgtccaag gcgtaggatt tcgctacatg accaagatgg
     8581 tcgctgatca actaggcgtg actggaacgg taaaaaacga agacgacggc tcagtcacaa
     8641 tcgaagcaat cggcaatgac gacatcatcc agaaatttat tgaagaagtc aagaaatcac
     8701 cgagcccaag tggacgagtg cagtatgtgg atgtccaaga agacccgatg attgaggaac
     8761 gaaagaaatt tgatgtggtg ggataaggtt gtgaaaaagc tggtctgcat caagcattaa
     8821 ggacaggttg tgaaaaatag ctctccatat ttttcaacag gctggactta atgccgtaga
     8881 tgcaagcttt tgaacaccgt caataaggtt gtgagaaagt cgcttagttt tgagtagtaa
     8941 ggagaatttt gtgaaaatgg ctttctacat tttttcataa aatttgactt aataccgaga
     9001 aactgacttt tgaacaccgt cgataaggtt gtttttgagg cgctttgcct cgagtattaa
     9061 ggagaaacat tt
//
LOCUS       DAZKAO010000108         8828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000108 DAZKAO010000000
VERSION     DAZKAO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.109"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2714
                     /gene="adhE"
                     /locus_tag="ACG8H8_002209"
                     /gene_synonym="adhC"
     CDS             117..2714
                     /gene="adhE"
                     /locus_tag="ACG8H8_002209"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP8987264.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2943..4049
                     /locus_tag="ACG8H8_002210"
     CDS             2943..4049
                     /locus_tag="ACG8H8_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8987265.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4066..5094
                     /locus_tag="ACG8H8_002211"
     CDS             4066..5094
                     /locus_tag="ACG8H8_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP8987266.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5100..5579
                     /locus_tag="ACG8H8_002212"
     CDS             5100..5579
                     /locus_tag="ACG8H8_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP8987267.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5595..6890
                     /locus_tag="ACG8H8_002213"
     CDS             5595..6890
                     /locus_tag="ACG8H8_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP8987268.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6901..7725
                     /locus_tag="ACG8H8_002214"
     CDS             6901..7725
                     /locus_tag="ACG8H8_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8987269.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7890..8789
                     /locus_tag="ACG8H8_002215"
     CDS             7890..8789
                     /locus_tag="ACG8H8_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987270.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2828 a         1534 c         1963 g         2503 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg
       61 tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg
      121 ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt
      181 tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag
      241 accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga
      301 tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg
      361 cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag
      421 atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc
      481 caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa
      541 atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag
      601 tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac
      661 atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag
      721 cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg
      781 taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga
      841 acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg
      901 tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt
      961 atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat
     1021 atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa
     1081 tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt
     1141 atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg
     1201 accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag
     1261 ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc
     1321 gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga
     1381 ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct
     1441 ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt
     1501 tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg
     1561 aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg
     1621 atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag
     1681 atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact
     1741 tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa
     1801 tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg
     1861 atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc
     1921 cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa
     1981 cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc
     2041 ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa
     2101 cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc
     2161 tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg
     2221 aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat
     2281 tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg
     2341 gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa
     2401 aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg
     2461 caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg
     2521 ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca
     2581 cacaagaaac actagataca accgtagacc gtatggctga attagcttac gaagaccaat
     2641 gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg
     2701 cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa
     2761 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
     2821 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
     2881 tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
     2941 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
     3001 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttggactttg
     3061 atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc
     3121 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
     3181 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
     3241 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
     3301 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
     3361 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
     3421 tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa tatttagaag
     3481 aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga acaggattgt
     3541 ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt tgggctcgtg
     3601 gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa ttatcgaaag
     3661 aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa
     3721 cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct tcttacgtag
     3781 aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat aaacgttatt
     3841 taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt gaacaaatcg
     3901 atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac agtttagatt
     3961 tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg gtgctttgtt
     4021 tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa aaaaacatca
     4081 ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg ctgcggaaat
     4141 aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac cttgcgcttt
     4201 gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4261 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4321 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4381 tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta tctattcaat
     4441 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4501 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4561 tatatggtag acggtccgat ccatacgccg gaagatttgg agggaaagaa gatccgtgtg
     4621 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4681 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4741 gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc ctatgatgag
     4801 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4861 gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4921 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4981 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     5041 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtga
     5101 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5161 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5221 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5281 tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5341 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5401 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5461 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5521 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5581 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5641 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5701 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5761 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5821 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5881 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5941 ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg gaagaaggat
     6001 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6061 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6121 tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat
     6181 tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg gctcatgctg
     6241 gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt atcggtggta
     6301 tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt tactccctgt
     6361 tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat atgttgtttc
     6421 aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc atgatgtctt
     6481 tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg ggcgtaacgg
     6541 ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg ggtatgttta
     6601 tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc gtcacaagtg
     6661 taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt gtaggctcca
     6721 tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag
     6781 aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc ttgtttttga
     6841 ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag gagaatagtt
     6901 atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat agaagatctt
     6961 gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg tctttcgttt
     7021 cctgaaacac cttctggcgc aaatgagatc aattctggga tgggaaatta tgtcaaacga
     7081 gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa tatgattcat
     7141 ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct tcgctatgct
     7201 tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa
     7261 tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc
     7321 ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg tttgaatcat
     7381 ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc ggactatcta
     7441 ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga acaagtccaa
     7501 ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca tttgaaagat
     7561 tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac
     7621 acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa
     7681 acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa agaagacgtt
     7741 ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc tcttcttttt
     7801 tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata ataaaattgt
     7861 gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag
     7921 tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa
     7981 ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta aagggagaag
     8041 cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc
     8101 attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag cgtgtctata
     8161 tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat ctatcggctt
     8221 gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag gaagtattga
     8281 agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt gaagacataa
     8341 aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta caaaaagaag
     8401 agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa atgttacaat
     8461 atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag cagttttttg
     8521 tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg tttcatcagt
     8581 atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac cgcagctcta
     8641 gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa gcattcaaga
     8701 gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa cagttggttc
     8761 attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac cgcagtagct
     8821 gctgttgt
//
LOCUS       DAZKAO010000109         8654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000109 DAZKAO010000000
VERSION     DAZKAO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.110"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..2633)
                     /locus_tag="ACG8H8_002216"
     CDS             complement(252..2633)
                     /locus_tag="ACG8H8_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP8987271.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2623..3270)
                     /gene="recU"
                     /locus_tag="ACG8H8_002217"
     CDS             complement(2623..3270)
                     /gene="recU"
                     /locus_tag="ACG8H8_002217"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP8987272.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3334..3873
                     /locus_tag="ACG8H8_002218"
     CDS             3334..3873
                     /locus_tag="ACG8H8_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP8987273.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3963..4382
                     /gene="gpsB"
                     /locus_tag="ACG8H8_002219"
     CDS             3963..4382
                     /gene="gpsB"
                     /locus_tag="ACG8H8_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP8987274.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4404..4771
                     /gene="rnpB"
                     /locus_tag="ACG8H8_002220"
     ncRNA           4404..4771
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8H8_002220"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4980..6146
                     /locus_tag="ACG8H8_002221"
     CDS             4980..6146
                     /locus_tag="ACG8H8_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP8987275.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6192..7688
                     /locus_tag="ACG8H8_002222"
     CDS             6192..7688
                     /locus_tag="ACG8H8_002222"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP8987276.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7769..8452
                     /locus_tag="ACG8H8_002223"
     CDS             7769..8452
                     /locus_tag="ACG8H8_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8987277.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8461..>8654
                     /locus_tag="ACG8H8_002224"
     CDS             8461..>8654
                     /locus_tag="ACG8H8_002224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987278.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2720 a         1571 c         1838 g         2525 t
ORIGIN      
        1 tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca agtaaatgga
       61 taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct aaatgttaga
      121 tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga
      181 tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg
      241 tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt
      301 gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac
      361 ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt
      421 gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag
      481 ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa
      541 gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag
      601 tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg
      661 ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct
      721 gaagtgacag gcgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc
      781 gtatagcccg caaataggat atctggatac accccagaag aaataccaag ttttgcatac
      841 tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc
      901 gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct
      961 gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg
     1021 aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca
     1081 taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta
     1141 ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag
     1201 aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg
     1261 gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct
     1321 acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa
     1381 tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa
     1441 tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt
     1501 ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc
     1561 atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt
     1621 tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct
     1681 gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg
     1741 tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca
     1801 tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt
     1861 ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca
     1921 gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca
     1981 gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc
     2041 aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc
     2101 ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc
     2161 tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg
     2221 atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg
     2281 atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg
     2341 cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct
     2401 aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct
     2461 gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct
     2521 tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt
     2581 ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct
     2641 ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg
     2701 cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga
     2761 tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata
     2821 aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag
     2881 gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt
     2941 gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc
     3001 tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata
     3061 gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga
     3121 aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa
     3181 ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata
     3241 aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag
     3301 caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg
     3361 tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt
     3421 gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta
     3481 cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa
     3541 gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac
     3601 tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa
     3661 gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata
     3721 cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag
     3781 tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc
     3841 agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt
     3901 ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa
     3961 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
     4021 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
     4081 aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
     4141 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
     4201 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
     4261 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
     4321 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
     4381 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
     4441 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
     4501 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
     4561 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
     4621 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
     4681 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
     4741 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
     4801 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
     4861 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
     4921 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     4981 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     5041 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     5101 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     5161 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     5221 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     5281 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     5341 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     5401 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     5461 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     5521 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     5581 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     5641 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     5701 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     5761 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     5821 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     5881 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     5941 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     6001 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     6061 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     6121 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     6181 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     6241 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     6301 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     6361 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     6421 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     6481 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     6541 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     6601 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     6661 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     6721 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     6781 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     6841 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     6901 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     6961 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     7021 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     7081 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     7141 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     7201 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     7261 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     7321 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     7381 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     7441 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     7501 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     7561 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     7621 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     7681 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     7741 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     7801 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     7861 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     7921 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     7981 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     8041 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     8101 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     8161 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     8221 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     8281 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     8341 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     8401 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     8461 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     8521 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     8581 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     8641 gaacaccgtt tatt
//
LOCUS       DAZKAO010000110         8458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000110 DAZKAO010000000
VERSION     DAZKAO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.111"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACG8H8_002225"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACG8H8_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP8987279.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="ACG8H8_002226"
     CDS             complement(591..785)
                     /locus_tag="ACG8H8_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987280.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            868..1476
                     /locus_tag="ACG8H8_002227"
                     /pseudo
     CDS             868..1476
                     /locus_tag="ACG8H8_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1496..2524
                     /locus_tag="ACG8H8_002228"
     CDS             1496..2524
                     /locus_tag="ACG8H8_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP8987281.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            2521..3279
                     /locus_tag="ACG8H8_002229"
     CDS             2521..3279
                     /locus_tag="ACG8H8_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8987282.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(3365..4105)
                     /locus_tag="ACG8H8_002230"
     CDS             complement(3365..4105)
                     /locus_tag="ACG8H8_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8987283.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4166..5419
                     /locus_tag="ACG8H8_002231"
     CDS             4166..5419
                     /locus_tag="ACG8H8_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP8987284.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            5714..5968
                     /locus_tag="ACG8H8_002232"
                     /pseudo
     CDS             5714..5968
                     /locus_tag="ACG8H8_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            6090..6416
                     /locus_tag="ACG8H8_002233"
     CDS             6090..6416
                     /locus_tag="ACG8H8_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP8987285.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            6484..7197
                     /locus_tag="ACG8H8_002234"
     CDS             6484..7197
                     /locus_tag="ACG8H8_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP8987286.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            7507..8359
                     /gene="licT"
                     /locus_tag="ACG8H8_002235"
                     /pseudo
     CDS             7507..8359
                     /gene="licT"
                     /locus_tag="ACG8H8_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
BASE COUNT         2897 a         1433 c         1672 g         2456 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta
      901 gaaattgtat taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca
      961 agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat
     1021 gccccgcctt ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc
     1081 ccttccaaac tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc
     1141 caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg
     1201 atcttaacga taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat
     1261 gtcctatatt tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt
     1321 gtcagaggac aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt
     1381 cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag
     1441 atgacatgga cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa
     1501 tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg
     1561 attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga
     1621 attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg
     1681 gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga
     1741 gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa
     1801 aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga
     1861 aataactgtc cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa
     1921 agagctggct ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc
     1981 taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg
     2041 aaatagcgaa aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag
     2101 cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg
     2161 cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa
     2221 aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg
     2281 aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg
     2341 ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa
     2401 ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg
     2461 agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa
     2521 atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa
     2581 agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa
     2641 ccagatgccg caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc
     2701 gatacgccgt ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac
     2761 caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct
     2821 gcattacaaa acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa
     2881 gtaacggata aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt
     2941 attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc
     3001 gtgattcgaa atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc
     3061 gctgaaggaa ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat
     3121 gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca
     3181 gtggaattat ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca
     3241 tggtcgttaa cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg
     3301 gagtggagcc gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta
     3361 ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga
     3421 tctttcgacc aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat
     3481 caattttctg caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat
     3541 acttttccaa ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag
     3601 ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc
     3661 gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga
     3721 tttgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc
     3781 tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat
     3841 tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg
     3901 cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat
     3961 tatcaatagt acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga
     4021 tattctcgat atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat
     4081 cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt
     4141 tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac
     4201 tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag
     4261 ctttggtttt atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt
     4321 tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt
     4381 tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt
     4441 ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact
     4501 acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct
     4561 caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag
     4621 ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct
     4681 tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat
     4741 cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga
     4801 agaatattac catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt
     4861 aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga
     4921 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
     4981 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
     5041 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
     5101 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
     5161 tacgatcaat ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa
     5221 tccgacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
     5281 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
     5341 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
     5401 gagcaaatca gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag
     5461 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
     5521 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
     5581 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
     5641 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
     5701 ggcgtggaga agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag
     5761 agagttagaa caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt
     5821 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt
     5881 ttggggagaa gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca
     5941 tacacatggt ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa
     6001 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
     6061 gtaagaaatg attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta
     6121 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt
     6181 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
     6241 cacaattaga acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg
     6301 cagacaacct tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
     6361 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat
     6421 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
     6481 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
     6541 ttattagtag gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag
     6601 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
     6661 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
     6721 tcgcagatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
     6781 atcaatttac cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
     6841 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
     6901 aaccctgaat tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg
     6961 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
     7021 gatacccaat tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc
     7081 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata
     7141 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
     7201 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
     7261 accaatttgc tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct
     7321 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
     7381 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
     7441 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
     7501 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
     7561 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga
     7621 gatttcgcca gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca
     7681 attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt
     7741 cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac
     7801 cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt
     7861 attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
     7921 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
     7981 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
     8041 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
     8101 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
     8161 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
     8221 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
     8281 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
     8341 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
     8401 taggttcacg aatgtttgtg actgtttttt tggctcttcg tcaaattgtg ttggcgaa
//
LOCUS       DAZKAO010000111         8424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000111 DAZKAO010000000
VERSION     DAZKAO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.112"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACG8H8_002236"
     CDS             complement(<1..505)
                     /locus_tag="ACG8H8_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987287.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..2108
                     /locus_tag="ACG8H8_002237"
     CDS             471..2108
                     /locus_tag="ACG8H8_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987288.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACG8H8_002238"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACG8H8_002238"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP8987289.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="ACG8H8_002239"
     CDS             complement(3184..3819)
                     /locus_tag="ACG8H8_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP8987290.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACG8H8_002240"
     CDS             complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACG8H8_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP8987291.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5754..>8424
                     /locus_tag="ACG8H8_002241"
     CDS             5754..>8424
                     /locus_tag="ACG8H8_002241"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8987292.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DAZKAO010000112         8251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000112 DAZKAO010000000
VERSION     DAZKAO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.113"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..1560
                     /locus_tag="ACG8H8_002242"
     CDS             235..1560
                     /locus_tag="ACG8H8_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP8987293.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1553..1903
                     /locus_tag="ACG8H8_002243"
     CDS             1553..1903
                     /locus_tag="ACG8H8_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987294.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1900..2070
                     /locus_tag="ACG8H8_002244"
     CDS             1900..2070
                     /locus_tag="ACG8H8_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987295.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2974..3249)
                     /gene="prgO"
                     /locus_tag="ACG8H8_002245"
     CDS             complement(2974..3249)
                     /gene="prgO"
                     /locus_tag="ACG8H8_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP8987296.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3221..4174)
                     /locus_tag="ACG8H8_002246"
     CDS             complement(3221..4174)
                     /locus_tag="ACG8H8_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8987297.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4786..6279
                     /locus_tag="ACG8H8_002247"
     CDS             4786..6279
                     /locus_tag="ACG8H8_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP8987298.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6414..6710
                     /locus_tag="ACG8H8_002248"
     CDS             6414..6710
                     /locus_tag="ACG8H8_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP8987299.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6811..7662
                     /locus_tag="ACG8H8_002249"
     CDS             6811..7662
                     /locus_tag="ACG8H8_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987300.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7683..7895
                     /locus_tag="ACG8H8_002250"
     CDS             7683..7895
                     /locus_tag="ACG8H8_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987301.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2865 a         1283 c         1553 g         2550 t
ORIGIN      
        1 atttttttct aatataacta agcaaaactg gataatacga cacttatttt caaattttca
       61 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
      121 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      181 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      241 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      301 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      361 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      421 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      481 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      541 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      601 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      661 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      721 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      781 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      841 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      901 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      961 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
     1021 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1081 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1141 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1201 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1261 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1321 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1381 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1441 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1501 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1561 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1621 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1681 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1741 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1801 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1861 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1921 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1981 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     2041 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2101 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2161 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2221 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2281 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2341 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2401 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2461 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2521 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2581 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2641 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2701 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2761 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2821 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2881 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     2941 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     3001 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     3061 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     3121 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     3181 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     3241 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     3301 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     3361 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     3421 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     3481 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     3541 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     3601 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     3661 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     3721 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     3781 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     3841 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     3901 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     3961 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     4021 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     4081 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     4141 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     4201 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     4261 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     4321 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     4381 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     4441 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     4501 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     4561 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     4621 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
     4681 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     4741 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     4801 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     4861 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     4921 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     4981 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     5041 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     5101 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     5161 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     5221 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     5281 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     5341 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     5401 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     5461 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     5521 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     5581 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     5641 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     5701 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     5761 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     5821 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     5881 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     5941 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     6001 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     6061 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     6121 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     6181 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     6241 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     6301 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     6361 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     6421 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     6481 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     6541 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     6601 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     6661 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     6721 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     6781 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     6841 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     6901 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     6961 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     7021 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     7081 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     7141 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     7201 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     7261 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     7321 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     7381 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     7441 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     7501 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     7561 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     7621 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     7681 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     7741 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     7801 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     7861 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     7921 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     7981 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     8041 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     8101 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     8161 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     8221 tcgtaagaga aattgatcga agatgtgacg a
//
LOCUS       DAZKAO010000113         8204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000113 DAZKAO010000000
VERSION     DAZKAO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.114"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACG8H8_002251"
     CDS             <1..615
                     /locus_tag="ACG8H8_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP8987302.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACG8H8_002252"
     CDS             612..1385
                     /locus_tag="ACG8H8_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP8987303.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACG8H8_002253"
     CDS             1382..1780
                     /locus_tag="ACG8H8_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP8987304.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2348
                     /locus_tag="ACG8H8_002254"
                     /pseudo
     CDS             1965..2348
                     /locus_tag="ACG8H8_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2442..3401
                     /locus_tag="ACG8H8_002255"
     CDS             2442..3401
                     /locus_tag="ACG8H8_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987305.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            3408..3701
                     /locus_tag="ACG8H8_002256"
                     /pseudo
     CDS             3408..3701
                     /locus_tag="ACG8H8_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3760..4200
                     /locus_tag="ACG8H8_002257"
     CDS             3760..4200
                     /locus_tag="ACG8H8_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987306.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            4212..4880
                     /locus_tag="ACG8H8_002258"
     CDS             4212..4880
                     /locus_tag="ACG8H8_002258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP8987307.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            4944..5567
                     /locus_tag="ACG8H8_002259"
     CDS             4944..5567
                     /locus_tag="ACG8H8_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP8987308.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            5623..6009
                     /locus_tag="ACG8H8_002260"
     CDS             5623..6009
                     /locus_tag="ACG8H8_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP8987309.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            6222..7613
                     /locus_tag="ACG8H8_002261"
     CDS             6222..7613
                     /locus_tag="ACG8H8_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP8987310.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2647 a         1341 c         1767 g         2449 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattcg ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact
     2401 caaaatagtt ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta
     2461 caacggatga acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa
     2521 ctgcccattg cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc
     2581 aaggaaaatc agccttagaa tattatcaac agtataagaa aaacaaatca aactgtggta
     2641 gacgtccgct tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag
     2701 gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta
     2761 ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca
     2821 agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc
     2881 atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca
     2941 ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag
     3001 ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc
     3061 aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg
     3121 aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc
     3181 caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg
     3241 gtttattgaa atctatggat ttcaattcag tagatgaatt ttttattcaa tctgtcgcat
     3301 ctaaacgaaa taatattcct agaaaatcac tggattatcg aacacctttg gaagtatttt
     3361 tgagttacgt aagtattgat gatctgtcta acttaatttg acaattaaga attctaaaaa
     3421 gctttttaaa agtgatcagt aaaaaacgga atctgatcaa aacgacacag ctgttgaaaa
     3481 aatatccagg tctgcttcac acgtctattc aatttcctca attgattacg atgatccatc
     3541 gttttatacg tatgcaacag ctaccatcta agaagctgtt tcttccattg acggacacat
     3601 ttcgagtagt caacagagaa gataaaaaga aagtgattgt gattgacttt accagaaatc
     3661 ggaatgtcct tcaccaagtt gcaatggaaa aggccaatta aggctttttc tttttggatt
     3721 tttttgtcat aataaatatt agatgatgga ggagataata tgatgcaaac atggttagaa
     3781 aggctaatac gatttcagca gaactatcac aaacttatgc agggaagata tgcgaaattt
     3841 gatcagttga atcgcggtct attgatcgct gcgcttgtct ttagtttgct aagtcgttgg
     3901 ttgccctata gaatcggaca attgttgttc atcgtctttt tcggatggat gatatttcgt
     3961 ttcctatcaa aaaaaatcta ccccagacta aatgagaatc aacgttattt gaaatatctt
     4021 gatagagtca aagaaaaata tcatcagata aaaaacaaaa agcaagtcaa aatggatcga
     4081 aaaagttata ctttttttga gtgcccgaat tgtcatcaaa agcagcgtgc acctaaagga
     4141 aaaggacgaa tacgtgtgac atgcaaaaca tgtggtataa aattcgaaac gaatgtttaa
     4201 gggagaaaag aatgaaatta gtattattac gtcatggcga aagcgaagca aattttgaaa
     4261 attactggac aggatggcta gatgtcgcct taacagaaaa aggaaaagaa caagcagcag
     4321 ttgccggtca aaaaatgctt gaagccggac ttcaattcga tgccgtttat acctctgttt
     4381 tgaaacgtgc gattcttacc ggacaagtcg cactagaaga aatgggtcag ttatggcttc
     4441 ctataataaa aacatggcgt ctaaatgaac ggcattatgg ggatcttgtg ggtaaaaaca
     4501 aagataagat gaaagaaata tacggaaaag atcaagtaaa aacatggcgc agaggatata
     4561 cggaagttcc gccgttaacc gcagaaaata gatttgaccg cagatatgat cacttggatc
     4621 cgcatttgat cccatacgga gaaagtctgg aaatgacagt gaatcggatc attcccttat
     4681 ggcaagatca tctagcacca aaactaaaag atcggcaaga tttactagtt gtgggtcatg
     4741 gtaatagcat ccgagcactg acaaaatatt tagaagatgt tcctgaagat caaatggata
     4801 cgatcgatat cccaaatgca caaccaatcc agtatacatt agcagatgat ttgacaattc
     4861 tggacaaaaa aatactttaa agaacacctt tatctaaaag tgagagatag aaaaaagaag
     4921 caaaggaatg gaacaaaagg aacatgaaaa attatatatg ggactttgat ggtacattgt
     4981 ttgatacgta tcccgcaatg gtagatggtg catggcaagc actaaaagat tttggtatca
     5041 gtatggacaa aaaagaaatc tacttcaaaa tgaaaaagta ttctactagt tatttgatca
     5101 atgaatcaaa cttgaatgcc agagagttta atgaattatt tcaccgatat gaaaaagaaa
     5161 gtacggaagt ttcccgtcca tttccagaaa cgaaacaagt actggaaatg ctaaaagaca
     5221 acggcggtcg tcatttcatc ctgacccacc gtttgacaga atcaacatgg ggcctgttaa
     5281 aagaacatcg attggcacat ctgattgagg aagtagtagg gatcgatcag gactttcctc
     5341 gtaaacctga tccagcatct ttaaattatt tgatcgatac ttttcattta gaaagaacag
     5401 acacgatgat gatcggcgat cgtcgtttag atatcgaagc agggaaaaat gcaggagtcg
     5461 ctacttgtct ttacgacatc gatcattttc tgggtgagat cccggcagat tatgtggtag
     5521 gaaatctgaa tgaaatcctt accttgcaga gcatcattaa aaagtagtat cccctttcaa
     5581 aggagatatg ttataatttt cataaaggag tgataaaatt atatggaaat cagaagacaa
     5641 aaagaagttg tagaagcatt tcattatgat atgagattgc cgaaccaaga agtagaaaca
     5701 gatttgaaag taggcttctc gcctttaaaa gaaacgatcg aaaactatcc gaaagaacat
     5761 agcatcgtag gagcacgctt agagttccgt ttagtgtttg atacatatgt tttatctgga
     5821 agtgtgagtc agatcaatca tatcctcaat cggaaaattg aaggtcaaaa tgatgtcaca
     5881 caagaagaag tggacgagct agtagcaccg ttattcgata tcgtacgccg aatgtcttat
     5941 gaggtgacag agatcgcctt agatcagcct ggggtaaaat tgaattttca atcagaaaaa
     6001 caagaataga cagtaaaaac atacctgcga tagattaaag ctatcacagg tatgtttttt
     6061 tattactaat tgtaagcaaa aagaatgtca tttcgataaa aatcgtctaa aatataaaag
     6121 tcactgaaaa aaattttttc aggaggatgg ttgaagaatc attctcggga acctttactt
     6181 ccttcgatgg acttgtcatt aataatatga ggaggagaaa aatgacaaca ctcatcacag
     6241 ttttagtggt tctagttttt gcggcattat tatatgtgtt ctatcgtatg caaaagcgcc
     6301 atacaaaatt ttcaacacgc gtctttacag cattaggagt tggtatcgtc tttggcggtc
     6361 tgcttcaatt gatctttggt gtagacgaca aagtcgttac tcaatcaatg gaatggatcg
     6421 ggattgtagg tcaaggatat gtatcattct tgcaaatgct agttatgcct ttagtgttta
     6481 tttcgatcat tggtgcattc acaaaaatga aagtttctga aaagctaggg aaaattagtg
     6541 cgactgtatt gacttcttta cttggaacaa cagctattgc agcattgatc ggaattggtt
     6601 caacaatgct gttcggactc caaggagcta aattcacaca aggagctgca gaaacagcac
     6661 gaatctctga actggcaaca cgtcaggaaa cagttgaaaa cttatcgatt ccacagcaga
     6721 ttttatcctt tatccctaaa aacgtttttg ctgatttagc aggtagtcgt gcaacaagta
     6781 cgattggagt cgttattttc gcggcatttg tcggaatcgc ttatctaggc gtgaaaagaa
     6841 aagcgccaaa agaagcagca ttcttttcag atttagtcga cagtctgtat aaaatcatta
     6901 tgcggatcgt gacgcttgtt ttgcgtttga caccttatgg agtatttgct ttgatgacaa
     6961 acgttttggc aacaagtgat tttgcagcta tcgtcaattt aggtaaattt gtcgttgctt
     7021 cttacgtagc attgcttgct gttttgattg tccactcact gattttgatt ggtgcaaaag
     7081 tcaatccaat caactatttc aaaaaagcct ttccggtatt gagctttgcg tttacttcac
     7141 gttcaagtgc tggagctttg ccattgaata tcgagactca gacaaaagct ttaggagtag
     7201 atgatgcaac agctaatttc tcagcaagtt ttggtttgtc tattggacaa aacggctgtg
     7261 ctggcgttta tccagctatg ttggcaacga tcgtcgcacc agcagctgga gtgaatgtct
     7321 ttagtttgga atttattttg atgcttgtgg caattgtaac aatcagttct tttggtgtag
     7381 ctggagtcgg tgggggcgca acatttgctt cactgatcgt tttaggggca atgaacttgc
     7441 cagtagcaat cgttgggtta gttatctctg ttgaaccatt aatcgatatg gcacgtacag
     7501 cagtaaacgt cagcggtagc atggtagctg gtatcgtgac aagtgcacgc atcaaagatt
     7561 tagatcgcga tgtcatcaat gatgattcta aagtgatcga tgtgaatgca tagtcaaaaa
     7621 gaggttgtga tagaagtggt cagctttaag aaataagaag aagtttccta aaaatagttt
     7681 ctcaaatttt tgtgaaattt cggcttattt ccgagaagct acttctgaaa caccgtgaat
     7741 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt
     7801 cctcatattt tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt
     7861 aagaaagagg tcgtgagcct gccgtttcac tctgagtcac aaagagcaat ttcaaaaaat
     7921 agcttctcat gtttgttgaa tattgtgtct tgtgaccgag gagttgtcag gagaacaccg
     7981 tttaccagag gttgtgactt gtttttagtc acgacctctt tttttattta ttccatataa
     8041 atacacatta tttattccga aaagtgaaag cgcaaataac aaaaacaacc aacttattta
     8101 ttattaaaga aaagcgtatt taagtaggac gattctgttg agagctttcg tagattttcc
     8161 cattttccta tggtaaactg acaaagttga gaatgttaga acag
//
LOCUS       DAZKAO010000114         8167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000114 DAZKAO010000000
VERSION     DAZKAO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.115"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      63..142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            140..1333
                     /gene="metK"
                     /locus_tag="ACG8H8_002262"
     CDS             140..1333
                     /gene="metK"
                     /locus_tag="ACG8H8_002262"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP8987311.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1463..2935
                     /locus_tag="ACG8H8_002263"
     CDS             1463..2935
                     /locus_tag="ACG8H8_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP8987312.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3018..3806
                     /locus_tag="ACG8H8_002264"
     CDS             3018..3806
                     /locus_tag="ACG8H8_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8987313.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3873..4541)
                     /locus_tag="ACG8H8_002265"
     CDS             complement(3873..4541)
                     /locus_tag="ACG8H8_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP8987314.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4656..6368
                     /locus_tag="ACG8H8_002266"
     CDS             4656..6368
                     /locus_tag="ACG8H8_002266"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987315.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6371..8143
                     /locus_tag="ACG8H8_002267"
     CDS             6371..8143
                     /locus_tag="ACG8H8_002267"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987316.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2507 a         1378 c         1797 g         2485 t
ORIGIN      
        1 atatttttta agcaaaaaaa tgaaaaaata cgagcagaaa actgttttat ctgctatact
       61 tggttcgttg caagttcccg aaaggatccg caggacggag atgccttgta accgaattta
      121 actaggggga atcattataa tggtagaaag acacttattt acatcagaat ctgtttctga
      181 aggacatcca gataaaattg ctgaccaaat cagtgatgca atcttggatg caattttaaa
      241 acaagatcca acagcacgag tagcgtgtga aacgtctgta acaactggtc ttgttttagt
      301 atttggagaa atatctacga cggcatatgt agatattcaa aaagtcgtac gggaaacaat
      361 aaaagaaatt ggttatacac gtgcaaaatt cggatttgat ggagatacag cagctgtatt
      421 ggttgcgatt gatgaacaat ctcctgatat tgctcaagga gttgatgaag cacttgagat
      481 ccgggatgaa gataaaaaag acgtattaga tgagattggt gctggtgacc aaggtttaat
      541 gtttgggttt gctgtagatg aaacaccaga attgatgcct ttaccaatcg ctttgagtca
      601 tcgtctggta cgacgcttgg cagatttgcg taaatcaaat gaattaacgt atttacgtcc
      661 agatgcaaaa tctcaagtaa cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga
      721 tacaatcgtt atttcaacac agcatgatga tgcagtagat aatgaaacaa ttcgtcatga
      781 tgtcatcgaa aaagtagtga aggaagttat tccagctgaa ttattagatg atcaaacgaa
      841 atattatatc aatccgactg gccgatttgt cattggtggt cctcaagggg atgccggatt
      901 aacaggaaga aaaatcattg ttgatacgta tggcggttat gctcgtcatg gtggcggtgc
      961 tttttctggt aaagatgcga caaaagttga ccgttctgcc agctatgctg cacgttatat
     1021 tgctaaaaac attgttgcag cagggcttgc tcgcaaagca gaagtacagc tagcttatgc
     1081 gattggtgtt gctcaacctg tttcgatctc aatcaatact ttcggtactg gaacggttcc
     1141 ggaagaagaa ctgattgctg cagtgagaga aaacttcgat cttcggccag caggaatcat
     1201 cgagatgctt gatttgcgtc gtccgattta caaacaaaca gctgcttacg gtcattttgg
     1261 tcgtacagat gtagatttgc cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag
     1321 tttagcaaaa taagttatat tcagctggaa cgatcgccaa gtggtggttg ttccagcttt
     1381 ttttatggca gttaacctct tgaaaaagat agtaattatt tatctatttt tagatcctgt
     1441 cataatgaaa gggaggaata agatgaaaaa agaaacaaat gttactttgg tcacagtgag
     1501 tattttcatt gcaacgttca tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc
     1561 aacgattgtc ggttcattac atggaatgga aatcatgaac tgggtatttt cgatttattt
     1621 gctgacaaac gctatgctta ctccgattta cggaaaactt gcggataaag tgggacggaa
     1681 accaatcttt atttttggga cattattgtt tattatcggt tcgttatttt gcggtttatc
     1741 tcaaagcatg attgggttga ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat
     1801 gatgccagta gctttgacga tcatcggcga tctttattct attgaaaagc gagcaaaagt
     1861 tttaggtttg aatagttcag cctgggggat tgccagtgtt tttggaccgc ttgcgggagg
     1921 attcatcgtt gaaacgatca gctggcactg gatctttttt atcaatgtgc cgatcggaat
     1981 tctattaatt attttgattt ggctatattt aaatgaacca aaaatcatac atgagtctaa
     2041 accaatggat atttttggta gttttagttt gatggccatg cttgtctcat tgctgttagg
     2101 gttccaatta ttaggagacg aaggaatgag tccgcttgtt tctgctttgc tgctaggtag
     2161 tatcgtaatg ttaggtattt ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat
     2221 gatgcattta ttcaaaaatc cactatttgt tgtggtcaac ttggctgcag cattggctag
     2281 tggttttttg atgggaatcg atgtttatat ccctatgtgg atgcaaggtg tattagggct
     2341 gaatgcagca attggtgggc tggtattagc accattatca ctagtctgga tggtcggttc
     2401 tttccttgca agcaagtgga tgactcaaat gaaaactcat tgggtcttga gaattggact
     2461 actaatcgca gctattgggg gcgcttggtt gtatcttatg cctttcagaa gtaactattt
     2521 atggtttttt gctatatcaa cggtcatcgg tataggctta ggtgtgacca tgactacctc
     2581 gactgtcagt gcacaaaata gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa
     2641 tacgttagtt cgaacaattg gtcaaacagt catggtttct atttttggtt tattgataaa
     2701 tggggtaact aaacgagaac ttgcagcagc aaatttaaca aatgatgcag atatcatgaa
     2761 taaactggtg aatccacaaa cggctaaata tattgatgcg gatattttgt taccattaag
     2821 aaaaattctt tatgatgggt tacattccgt ttatctagtt ggcttgttac tcgtgatcat
     2881 tgctttggtc ttaacgcttt tcgttcatga aaaaagtggt aaaataaaac agtagtttcg
     2941 gttaagatat gtacgtagcc gaatcattca aatttaaagt tattcaaaaa taacttctta
     3001 ctaaggaagg cgaaaatatg gcatttattc aagcaaatat ttattcgaat gtgttagaaa
     3061 tggaagtcaa tctaaatgtg atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt
     3121 cagagcaaga gacaacggat atacctgttt tatatttact tcatggaatg ggtggcaatc
     3181 atagtgtatg ggaaagacgt acgtcgattg aacgttatgt agcaaactac ggattagctg
     3241 tcatcatgcc atctactgat cttggctggt acacagatac agtttatgac atgaagtatt
     3301 ggacattcat tgcagaagag ctacctgaga tttgtcatga attattcccg caattaacaa
     3361 gaaaaagaga aaaaacatat gccgctggat tatcgatggg cggatatggc gcgttaaaac
     3421 taggccttgc aaaatcagaa aatttcgcag cagtaatctc tctttcaggc gctgtttctg
     3481 taggaaatcg aatggatgat cttttgatgg ttcgtaaagc atctttttgg gaagggatct
     3541 ttggaccatt ggatcaaatc gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac
     3601 tagtaaaaag cggaaaacca gttccaagat tttatatggc ttgtggggaa tctgattttc
     3661 tttatcaggc aaatcaggaa atggtatctg ctatgcagga acaaggcttg gcggtcacct
     3721 ttgaacaagg acctggcgat cataactgga tcttctggga ccaatggatt caacgtgcat
     3781 tggcgtggct aatggaagaa aaataaaaaa agagtcttaa aaagatagct cctatagaga
     3841 tcacaaacat agtgatctct ataggagctt ttttactgct cgttttctct tcttttattt
     3901 tgttggcata agccttcaat ataggtttgt gcactcgtta gaaagagtgc tttttcattc
     3961 aattctgcat gggactgcat actttcttta aatccctcta attgattttc aactacataa
     4021 ttacgcataa atagaactgt ttctttcaaa aattcatttc cgtccataat tttttgtcga
     4081 agctgctgtt catgctgctg atccattaac tgtccaatca ataaattgtg gagagaggca
     4141 atggcctgat gactttgggt gactgaaaat ccttcacgaa caagtaattg gatcatttgc
     4201 tccaaatgtt ctaatcgact cggaaaagcg ggcacagtat tgatttctaa ttctgcccca
     4261 caagggaaac gtgtataaag gtcatagtaa ttttccatat actgcagcaa ctgttcattc
     4321 caaggcaaag ttggatctgg aagcgctaat tcttcttcca ttgtttcagc cattttttgc
     4381 aagagctgtt gtttgttttt gaaataccaa tataaagcag gtgcttgaac atgaattctg
     4441 tttgcgatgt ttcgcatgga aagctgcgaa agagcttgtg tttcagccag gatatcaaag
     4501 gcggcctgaa tgatgattgc ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt
     4561 gcattatctt tttaaacatg gtaaattgtt agtcggtcta attttacagt gttaaattag
     4621 tcctgtcaat ttaaacaaag gggtgtaatc aacgaatgaa attaatctta cgacatgcga
     4681 aaaaatataa agtatctgtt tttatttcct tgctgtccgt agcagtcatg gttgcagcag
     4741 cattatggca acctaagtta ctacagcaag tattggaagc tattattaca gaagataatg
     4801 atgaaatgaa aacaattggt atttacctga tcagtctggc tttgctagga ttagccgcag
     4861 gtgtaacgaa cacgattttc tctgcaaaag tagctcaagg cgtaagtgca gatatccgag
     4921 aagaagcatt tcgaaagatc cagacatttt catttggaaa tatcgaacag ttctctgctg
     4981 gaaatcttgt tgttcgtcta acaaatgata tcacgcaaat ccaaaattta gtgatgattt
     5041 ctttgcaatc actttttcga attccattct tatttattgg tgccttcatt ttagctatga
     5101 tcacgatgcc gcaattatgg tggatcatcg tagcgcttat cgttacggta tttctgatta
     5161 cggcactttc atttacaagg atgggcaagc acttcatgat cattcaaaaa ctgattgatc
     5221 gtgtgaatag cattgcaaaa gaaaacttac tagggattcg tgtagtgaaa tcttttgtac
     5281 aagaagaaaa ccaattgaag tcattcagca aagtaagtga ggacttaaca aaacacaaca
     5341 tcatagtcgg tacattattt tctgtgatga tcccgtcctt catgctggct gccaatttgg
     5401 ctgtggttgg ggcgatcttt tttgtgagcg atcttgttaa agatgatcct acgttaattg
     5461 gtggaattgc ttcattcatg aactacttga tgcagattat gatggcaatc attattggcg
     5521 gaatgatgat gatgatgact tcaagagcag ccgtatcgct gaagcggatt gcggaaattt
     5581 tagatgctga accagatctt acttatttag acgttccaga acaagagttg acgggttctg
     5641 tcacttttga ccatgtttct tttcgttatc ctggtgagga tacagatacg cttaaagata
     5701 tcagcttttc tatcaaacct ggagaaatgg ttggtatcgt cggggcgact ggtgccggga
     5761 agtcaacact agcacaattg atccctcgtc tatttgatcc gacagaaggc aaagttgaag
     5821 taggtggtgt agatcttcgt caagtaaatg aaaagagttt aagacaaacg gtttcctttg
     5881 ttttgcaaaa agcaatttta ttctcaggaa cgattgctca aaacttgcgc caagggaaaa
     5941 agaatgcaac agaaaaagag atggaacatg cttcttctat tgctcaagca aaagaattca
     6001 tcgaaaagtt atctgatcgt tacgaagcac caatagaaga acgtagcagt aacttttctg
     6061 gtggtcaaaa gcaacgttta tccattactc gtggcgttat cggtaatcca aagatcttga
     6121 tcctagatga cagtacgagt gcactggatg ctcgctctga aaagctggtc cgagaagcac
     6181 tcgatcgaga tttgaaggga acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg
     6241 tcaaagcaga ccgcatctta gtattggatg aaggcagatt agtcggcgaa ggaacccatg
     6301 aggaattggt agctggaaac cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg
     6361 aggagtaagt atgactgatc taataaaagc aagtaaattt ttctatcact atttgaagcg
     6421 gtacaaactc tcctttttct ttatctttat cacagtgatc attgctacct atctacaagt
     6481 aaaggcacct caatacatcg gggaagcttt tcaagaatta gctaattacg taggaggctt
     6541 gatgcaaggc gtagacgata aaagcaaatt tgtttctgta atatggaaac tcttgctgtt
     6601 ttatgtacta gctagtgcag ccaactttat ctatagtatt ttgttcacac aagtagtagg
     6661 gaagtcaacg aaccgaatgc gtatcggtct attcaacaaa ttggaaaaat tgacgatccg
     6721 tttctttgat tctcatcaag acggtgaaat tttaagtcgt tttaccagtg acttagataa
     6781 tattcaaaat agtttgaacc aagctttgct tcaagtcatc acgaatgctg ttttacttgt
     6841 cgggatcttg atcatgatgt tccgccaaaa tgtagagtta gcttgggcta caattgcttc
     6901 cactccagta gccgttttga tagctgtctt gattatccgc aaagcacgga aatatgtgga
     6961 tcttcagcaa gatgaagtgg ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca
     7021 acgtgtcatc atcacaaatg gcttacaaga agaaacgatt gaaggattct tagagcataa
     7081 tcaaagtgtt cgcgaagcaa ccttcaaagg acaagcttat tctggtctac tatttccgat
     7141 gatgcaagga atgtcactgg tcaatacagc gatagttatc tttttcggcg gctggttagc
     7201 attaaatggt gatttggaac gttcggtggc attaggctta gttgtgacat tcgtccagta
     7261 ttcacaacaa tattaccaac cactgatgca gatttcatct ggatacagca tgatccaatt
     7321 agcgattaca ggggcaagac gattgaatga aatatttgat gaaaaagatg aagtcaaacc
     7381 agaaaacggt aaagcatttg atggtgttga aaaaggtctt gcgttgcagc acgtagattt
     7441 tggttattct ccagaaaaac tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga
     7501 aatggttgct cttgttggtc caacaggatc ggggaaaacg acgatcatga atctgctaaa
     7561 ccgtttttac gatgtcaatg atggttcagt agtcatcgat ggaatagata tccgagatta
     7621 tgatcttgat gcattacgta gacatgtagg aatcgttttg caagaatctg tattgttctc
     7681 cggaacgatc aaagacaata tcacattcgg caaaccggac gcttcagatg aagaagtcat
     7741 caatgcagca aaacaagcga atattcatga cttcatcatg acgttagaaa aaggttatga
     7801 tacagagata tccgaagaaa ataacttatt cagtacaggt caaaagcagt tgatcagtat
     7861 tgctcgaacg atcatcacaa atccatcttt attgattttg gatgaagcaa caagtaatgt
     7921 agacacggta acagaagcaa ggatccaaaa agcaatggat gaagcaatca aaggaagaac
     7981 cagctttgtg attgctcacc ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt
     8041 gcgtgatggg gaagtcattg aagaaggcag tcatcatgag ttattagcag aaaatggatt
     8101 ttatgcggaa ctttatcata atcaatttgt atttgaaagt taaattaaaa gaacctcgtt
     8161 ataacgg
//
LOCUS       DAZKAO010000115         8059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000115 DAZKAO010000000
VERSION     DAZKAO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.116"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG8H8_002268"
     CDS             387..575
                     /locus_tag="ACG8H8_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP8987317.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG8H8_002269"
     CDS             653..1666
                     /locus_tag="ACG8H8_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8987318.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACG8H8_002270"
     CDS             complement(1733..2767)
                     /locus_tag="ACG8H8_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8987319.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACG8H8_002271"
     CDS             2882..5107
                     /locus_tag="ACG8H8_002271"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP8987320.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACG8H8_002272"
     CDS             5125..7008
                     /locus_tag="ACG8H8_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP8987321.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPQEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACG8H8_002273"
     CDS             6989..7963
                     /locus_tag="ACG8H8_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP8987322.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2604 a         1468 c         1705 g         2282 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccacaag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg aacaagagag
     8041 taaaaaagtt ctcagatac
//
LOCUS       DAZKAO010000116         8026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000116 DAZKAO010000000
VERSION     DAZKAO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.117"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            248..949
                     /locus_tag="ACG8H8_002274"
     CDS             248..949
                     /locus_tag="ACG8H8_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8987323.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1521..2537)
                     /gene="tsaD"
                     /locus_tag="ACG8H8_002275"
     CDS             complement(1521..2537)
                     /gene="tsaD"
                     /locus_tag="ACG8H8_002275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP8987324.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2534..2998)
                     /gene="rimI"
                     /locus_tag="ACG8H8_002276"
     CDS             complement(2534..2998)
                     /gene="rimI"
                     /locus_tag="ACG8H8_002276"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP8987325.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3005..3547)
                     /gene="rimI"
                     /locus_tag="ACG8H8_002277"
     CDS             complement(3005..3547)
                     /gene="rimI"
                     /locus_tag="ACG8H8_002277"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP8987326.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3531..4253)
                     /gene="tsaB"
                     /locus_tag="ACG8H8_002278"
     CDS             complement(3531..4253)
                     /gene="tsaB"
                     /locus_tag="ACG8H8_002278"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP8987327.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4444..5424)
                     /locus_tag="ACG8H8_002279"
     CDS             complement(4444..5424)
                     /locus_tag="ACG8H8_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8987328.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5448..6932)
                     /gene="galT"
                     /locus_tag="ACG8H8_002280"
     CDS             complement(5448..6932)
                     /gene="galT"
                     /locus_tag="ACG8H8_002280"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP8987329.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6944..7933)
                     /gene="galE"
                     /locus_tag="ACG8H8_002281"
     CDS             complement(6944..7933)
                     /gene="galE"
                     /locus_tag="ACG8H8_002281"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP8987330.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2259 a         1711 c         1412 g         2644 t
ORIGIN      
        1 gaataaatta aaaaaataaa ttatattagc gttgttagcg tttactatat gtattattgt
       61 tgttttttat acacaaatca ttcgcttcaa aacaaattat tcaataccat tgtatatttt
      121 ttacataata acaaattata tttgtaaagt attaaaactt gtttctaatg ttggtatgtt
      181 aaaactttac aaattcttta aaaaaagtga caataaatat agtgaacaat aaggagagga
      241 ctttttaatg cgtgttaata aaatcgattt tcacttttca aaactcagat atcaacctga
      301 ttttgaacat ttctcggatg aagagttcca acagatcaag gaacatactg ttcttcgttc
      361 ttataaaaaa ggacagatcc ttttcgacga tggtgatccc agacaacggt tgtatatctt
      421 gaccaaagga ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt actactatga
      481 ctatgtgaat acaaataacc tttttcctat gtcaggaatg tttggagaag agacctacac
      541 ttatacagct caagcgatga ctgatattga aacattctac cttcctgttt cctactatga
      601 aaaattagca cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa tttctaaagt
      661 agttgaaata catgaattgc gtatccaaat cggattgacc tctagcgcct ttgaccgggt
      721 aaaacaaaat ttattcattt tgaaagaaga attgggcgta ccaacaaaag atggtcatat
      781 caccattcct tatccgatca ccttaaaaga attagcagtg aatagcggca cgacacgtga
      841 aactgctgga caagtaatca aacaactacg tgattccgga ctgttagatt atgacaaaaa
      901 gcgttttact ttctgtaaga cagctactac tccagctatg agagaataac gcgaacaaaa
      961 ataagcagtg accctgatat ttatcagggt cactgcttat tttcctccct aaaacttctg
     1021 aaagatccgg cagttttaga tcactgggca agactcaaag attttctatg tgtgtttcag
     1081 ttatgtaata ttcactgttt gcaaaaaata ctgatagatt aactagagga agcgtcttgt
     1141 cctcatccga cctagattat tcgatattcc tcgacaataa acgattaatc agtgagggca
     1201 atcagaggaa cggataatga tgaataagct gtaaaaaata gggctttgac aaaagcgtgt
     1261 cacagccctt atttcgatta aacggtgtgt acctgtcaac tcttcggtca caagtcataa
     1321 tgttcataaa catgaggagc tgttttttga aattgttcct tgtgaatcag agttaaacga
     1381 caggcttacg aactcttgat taaacggcgt tcaaaagctt gcatacgcgg taataagaac
     1441 agccaggtca aaaacatgag aagccgtttt tcctgggctg ttcttattac ttgatgcagg
     1501 gcagattttt cacaacctct ttattcagat aaaaccaagc ttggatcagc attcaactga
     1561 tagctagcaa aatttctttt ttgcatttcc acatgtgctg ctgctccaat cattgcagca
     1621 ttatctccac aaagtcgaag agggggaatc actagttcta cctcagggag tttagttgcc
     1681 aaagcatcct gcaatccttc acgcaaccct tgattggccg ctacacctcc agctacaatc
     1741 agttgcttga ctggataatt ttgacaagct cgaagtgttt tagtaatcaa tacatcgatc
     1801 acacttgctt gaaagctagc tgctaaatca tttttgtcta gatcttctcc acgctgttgt
     1861 gcattatgca ccaaattgat aaatgcactt tttaatccgc taaaactgaa atcataatta
     1921 tcttcatgga tcatcgctcg tggaaaatgg taattatctt ttccctgatg agctagctga
     1981 tcgatttctt ttccgcttgg gtaagaaaga cctaatactc ttccaacttt gtcataagct
     2041 tctcctgctg cgtcatctct tgtctcccca atgatttcat atgatccatc ttcttgcata
     2101 tagactaatt ctgtatgtcc tccgctaaca agaagtgcca tcaatggaaa ctggaatggt
     2161 ttaaccagac gggctgcata gatatgccct gccatatggt ttacaggaat aagcggcaat
     2221 tggtgtgccc aagcaaaagc cttagctgca gaaataccaa tcagcaacga gccgacaagt
     2281 cctggtccat aagtgacagc gactgccgat aaatcttctg ccgacacacc agcttctacc
     2341 agtgcatctt ccaagcataa ggtgatttgt tctacgtgat ggcggctagc tacttcagga
     2401 actacgccac caaaacgttt atgactgttt atttgagaag caacgatgtt agataagatc
     2461 tctgttccat ttctaacgac cgctacactc gtttcatcac aacttgattc aatcgccaga
     2521 atcaattctt cattcatttc atcgtctccg tttttaactt tctgctcata atgatcgcat
     2581 cttccgtcgg attgcgataa tatgatttcc gtttacctaa tatacgaaac ttttctgatt
     2641 cataaagttt tcttgctgct tcattagata gacgtacttc taaaaaaaca tgcagcgaat
     2701 cttttatttg ttccgtaatc agtaattgta acaaatgccg tgcatgccct tttccttgtt
     2761 ctgaaactgc aacagcaata tttgtgatct cgacttcatc tattactttc gagtatccaa
     2821 taaagccaca gatctggtta tcttcaacta aaagcagata atcagagtga ggctgttgga
     2881 tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg tggataagca ttttcgctaa
     2941 tcatccatac gcattctgcc aagtacgctt catcaaaatc tttttttctg caaatcaact
     3001 aatctcactc attttctaaa agttttatca tatccaacgc atcttctttt gtttcagtgt
     3061 aatatctttt tttgatcgtt gttgcttgaa aacctaactt ccgataaagt ctttgtgcat
     3121 cttgattact catacgcact tctaatgaca tcgtctcaca gcgattcatt atagcaaagt
     3181 tctcgatttc gtcaataagc agcgaaccga ttcgtttacg ctgatatgcc ggtcgaacgg
     3241 caatattcgt gatatggcaa tctaaaccga acgctcttga accgataaaa ccaatcattc
     3301 ttccattatc aaagatacct agatatagat gaggaagcgg tgacttcaat tctgatataa
     3361 aggcactttt tgtccatggc aattcgccat tatagacttc tcgttcgaca tccagcagct
     3421 ctttgatatc ttctgtagtc aattctctta gtacgtaacg acgatcgatt tgttcgattg
     3481 ttttttctgt ataaatcatt ttatcttgaa aaaagcttct ggcatacgct ctaaattttt
     3541 tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc atttttcttc tgcttctacc
     3601 cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa ctggttcagc taaagcacct
     3661 aattgagcca cagttacacc acttggaata tcccatgctg gaactgtatt aatcatcgca
     3721 ctaggcaata tttcctttat ttcatagaag aatttgtgac aatcactccc gacgaaataa
     3781 atagcttcat tcagcttttg tagttgtgtg aacaaatcag aagcagaaat atgtacatct
     3841 tcaagtacaa tttctaatct attttctttc cagcggtatg ccccagcgta tacatttttt
     3901 cttcgagcat caaacatggg cacaataatt ttatccacac cgatgcaatt agccgcgatg
     3961 gttttcagac tagatactcc tactagctct ttatctaatg tataagcaat cgtttttgct
     4021 gtcgttacac ctatacgaag ccctgtataa gaaccgggac catcagaaac cgcgattcgg
     4081 tcaatgtctt ttggacttat ttgaagatct gcaaacaatt ggtcgattgc aggcatcagt
     4141 gttacactgt ggttgcgttt gatatttgtt tgtatttgtc caagaatttg ttcgtcttct
     4201 accacaccga tcgccaaagt ttggtttgca gtatctattc ctaatgtaat catatatttg
     4261 atcctttctg ctatgacatt ctcgccttcc attgtagcat acttttttat tctgaggatg
     4321 aaaataccaa aggcaaaact agtacaatga tacatgatta tctgctggcg ggcaaatttt
     4381 gccccttcct gaaacacaaa aaagctgcgg tatcactcca ttttcccaca gcttttccat
     4441 cttttatttc gttgattctc gaataatcaa ctctgtttcc acaataatct ttcttgaaac
     4501 cggtgctttg gaattactta attgaagaat cgtctccact gctagctctc ccatccattc
     4561 ggtataaact tttacagtag aaagcgaggg cgttacatat ttcgctacac tgacatcatt
     4621 gaagcccatc acagaaatat cttctggtac cactaaacct ttttcttgaa tcgctttcag
     4681 tgcgccaatc gctaatgcat cattagctgc aaaaagcgca tcaggaacat gcggattcgt
     4741 ttttaaatac tgcttcactg cttgatatcc tgactcgaca gaaaaatctg cttcgataat
     4801 tggaccttcg tcgtagcctt tttctgccaa aacttgttga aatgccataa ttctgggatc
     4861 ttttagccgc tgatggtttt tcttcgtata ttctgttcca gcaagcatag cgatatgttg
     4921 cgctttatgg gagacaaagt gttcaatgac tttttgaaca cttgacttaa agtcgacaac
     4981 tagagagtcg attccttgag aagtgccgtc agaatctacc aaaagcaagg cattttggga
     5041 tcctttcatt tctaataact gagcttcgtc gaattttcct agtgcgatcg ttccatctgc
     5101 cggaagatca ctaatggaat cccacgtttc tttcaataaa gtaacgtttg cttcttctgc
     5161 ctttttttcg attcctaggc gaatagataa ataataaaga tcttcaagct cttccgtatc
     5221 gtcgtaccac tggatcaggc gaagcgtttg cttagtattt gctgcatttt tacgatgttt
     5281 cgtataattt aagcgttctg ctgcttcaaa aattttttgc tttgtggcta agccaacgga
     5341 aagatcagga tcatagttga gaactcttga aacagtagca ggagaaactc ctgccaactg
     5401 cgcaatgtct ttaattgttg ccatttgtct ctcctccttt tttacgttta taaaatcgca
     5461 gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct tgaatactcc agcgtcttcc
     5521 agtacacgtg caaaaacctg acctacagat ttttgtacga catcggtgac cgtatctttc
     5581 gtcaaatcag gatgttcttt ttttaattgt tctgcccacg gacgatgata ttcagccatc
     5641 tggttttctt ctcctaataa gaaatgctct acttctacta gttctggttt caaacgaggt
     5701 ggtaaaacag caagccccat tacttcgatc aacccaatgt tctccttttt gatatgttgg
     5761 acatctttat gtggatgaaa aatcccatca ggatgttctt ctgaaacatt gttgtctcga
     5821 aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg caattggcgt aatcgtatgg
     5881 tgtggtgtac catctgctga aaaagctctg atagatactg tttcatccga atatgctttc
     5941 catttttcca atactgagtt agctgcttca ataagcaagt ttttctctgt tgattgcaaa
     6001 cggatcacag acatcggcca tttcacgact cctgcgatga cttgcggata atcttctagt
     6061 ttgataagtt tttctatcgg tgcttttgcc ataggaaatt catgacgtcc tgcctgataa
     6121 tgatcatgag aaagaatcga tcctccaaca attggtaagt cagcattcga accaacaaag
     6181 taatgaggca agacttcagt aatccgaagc agtctagcaa atgtttcctt tgtgattttc
     6241 atcgggcggt gttgttctga taaaatgatc gaatgttcat tgtaataagc ataaggggaa
     6301 tattggaatc cccagctttc accgtctaga ttcattcgaa taatccggtg attggtgcgg
     6361 gctggatagt tcaagcgacc tctatatcct tcgttttcca tgcaaagcat gcaggtagga
     6421 tagtctactt ttaccgcctc gcgttctgct gcgatttgtt ttggatcttt ttctggctta
     6481 gataaattga ttgtgatttc cagttcacca tactccgact ctgccggaaa tttgatgttt
     6541 tttgcgatgg ctcttgtttt gatgtagtca tttttcttac ttaaattaaa gaaatactca
     6601 gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga atgcattgac gactgaaggt
     6661 ggcggtgtga gaaaatccat taattgcgct tcaaaaatct cttgttctgc tggcaattct
     6721 gaaagaacac cattttctct tgccttagcg acaagctgat ccaataagtc tacagattct
     6781 tccttaactg gtcttacttc aatgctttct agagcatctt ctcctaccat ccctaaaaga
     6841 cgattctgta aatataagcg gtccatttcc atccatccgc cagcttcgat cgcaagtgtg
     6901 gcaaaatcac aaatcgtttg actgattgac atatctcatt cccctattct tcataaccat
     6961 ttggatggct gacatgccaa tcccatgctg ttttgataat cgctttgata tctgtgtatt
     7021 caggtttcca gcccagaatc tctctcgctt tttcacttga agcgactaat cggctaggat
     7081 ctccagctct tcttggtgct atttctgctg gaatttcttt tcctgtcact tctctagctg
     7141 cttctagcat ttccttgact gagtatcctt tgctgctgcc taaattgaag aagttgcttt
     7201 catttccttc tttcaagtat tccaaagcta aaatatgtgc agcaatcaaa tcttctactt
     7261 gaacataatc tctgatacat gttccatctg gtgtatcata atcgtctcca taaacagcca
     7321 aggcttttct ttgaccaaga gctacttgta aaatgattgg gacaagatgc gtttctggtg
     7381 tatgatcttc tccgatagat gcatctgctt ttgctccggc tacattgaag tagcgcaaag
     7441 cgacatagcg catgccatat gcttggtcac accacttcat cattttttcc atcatcaatt
     7501 tactttcacc atacggattt ttaggattag ctggtgtatc ttctgtgatt ggagactctt
     7561 tcggctctcc ataagttgct gcagtagaag agaatacgat tttattcacg ttgaactcgt
     7621 gcatgacttc aagtaagatt tgcataccgt atacgttgtt gttgaaatac atcaatggtt
     7681 tttcgacaga ttctcctact aaagagcttg cagcaaaatg gattactcct tcaatcggtt
     7741 ccttttcaaa tacggaacgt agaaattctt tatcccggat gtctccttca taaaaatgag
     7801 catctggatg tactgcttgt ttgtgacctg tgagcaaatt atctaccaca gctacttgat
     7861 accccttttg aactagctgg tctacagcgt gcgaaccgat ataaccggct ccgcctagta
     7921 ctaaaattgt cataaaaaac actcctttga ttagtaaaat tgtgtttgtt gattctatat
     7981 aatttaaaaa acgaagttta cttttatggt gaaaacaaac aaagta
//
LOCUS       DAZKAO010000117         7982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000117 DAZKAO010000000
VERSION     DAZKAO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.118"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..2010
                     /locus_tag="ACG8H8_002282"
     CDS             244..2010
                     /locus_tag="ACG8H8_002282"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP8987331.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2112..3371
                     /locus_tag="ACG8H8_002283"
     CDS             2112..3371
                     /locus_tag="ACG8H8_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8987332.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3518..4819
                     /locus_tag="ACG8H8_002284"
     CDS             3518..4819
                     /locus_tag="ACG8H8_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8987333.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4822..5673
                     /locus_tag="ACG8H8_002285"
     CDS             4822..5673
                     /locus_tag="ACG8H8_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP8987334.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5686..6501
                     /locus_tag="ACG8H8_002286"
     CDS             5686..6501
                     /locus_tag="ACG8H8_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP8987335.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6652..7818
                     /locus_tag="ACG8H8_002287"
     CDS             6652..7818
                     /locus_tag="ACG8H8_002287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8987336.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2603 a         1372 c         1590 g         2417 t
ORIGIN      
        1 tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga
       61 cactaaatag tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc
      121 aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg
      181 ttgtcttttg cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa
      241 tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg
      301 aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg
      361 ctatgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg
      421 aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca
      481 agtcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt
      541 gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga
      601 ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta
      661 tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac
      721 actttacctt ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa
      781 ggtgtgatgg atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt
      841 cccatattta aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat
      901 cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg
      961 atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa
     1021 gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc
     1081 ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat
     1141 acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa
     1201 tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt
     1261 gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa
     1321 atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa
     1381 ggggatgaat tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt
     1441 gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt
     1501 tacagagatc aggtgaacga aggaacattc aacttattag attctcatga tactgccagg
     1561 atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt
     1621 cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat
     1681 gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc
     1741 ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt
     1801 tctactcagt ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta
     1861 gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag
     1921 gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata
     1981 cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt
     2041 ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg
     2101 aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag
     2161 gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg
     2221 atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa
     2281 taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt
     2341 tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga
     2401 tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac
     2461 tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa
     2521 tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg
     2581 atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt
     2641 ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt
     2701 cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt
     2761 cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt
     2821 tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc
     2881 agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat
     2941 acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc
     3001 cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag
     3061 acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg
     3121 aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg
     3181 atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc
     3241 cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt
     3301 caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac
     3361 aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag
     3421 ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag
     3481 cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa
     3541 tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa
     3601 tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat
     3661 gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga
     3721 agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt
     3781 cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga
     3841 gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt
     3901 tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt
     3961 cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag
     4021 tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg
     4081 taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt
     4141 gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa
     4201 acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat
     4261 gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt
     4321 cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg
     4381 gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat
     4441 ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg
     4501 agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc
     4561 gccaacattt attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt
     4621 taacaatggc ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc
     4681 ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac
     4741 gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc
     4801 gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga
     4861 ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat catctatccg
     4921 cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat
     4981 ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg
     5041 acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta
     5101 gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg
     5161 atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc
     5221 atggctttct tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc
     5281 ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat
     5341 ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta
     5401 ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt
     5461 ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc
     5521 ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg
     5581 gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt
     5641 accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg
     5701 ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta
     5761 tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg
     5821 actctttttt atgccaatca gatccaagcg attccattag aacaatttct atccgttcat
     5881 tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg
     5941 ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta
     6001 tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg
     6061 atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc
     6121 caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat
     6181 gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt
     6241 gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc
     6301 caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga
     6361 ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta
     6421 agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc
     6481 aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa
     6541 atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct
     6601 tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag
     6661 aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat
     6721 tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat
     6781 atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc actaggagaa
     6841 gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct
     6901 tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa
     6961 gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac
     7021 gacttttata aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca
     7081 cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc
     7141 tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc
     7201 actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac
     7261 gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta
     7321 atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc
     7381 gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa
     7441 ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct
     7501 tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat
     7561 aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa
     7621 ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa
     7681 atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt
     7741 gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc
     7801 ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct
     7861 aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag
     7921 cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat
     7981 ga
//
LOCUS       DAZKAO010000118         7949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000118 DAZKAO010000000
VERSION     DAZKAO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.119"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..999
                     /locus_tag="ACG8H8_002288"
     CDS             136..999
                     /locus_tag="ACG8H8_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8987337.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1128..2750)
                     /locus_tag="ACG8H8_002289"
     CDS             complement(1128..2750)
                     /locus_tag="ACG8H8_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8987338.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2999..3520
                     /locus_tag="ACG8H8_002290"
     CDS             2999..3520
                     /locus_tag="ACG8H8_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP8987339.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3706..5436)
                     /gene="spxB"
                     /locus_tag="ACG8H8_002291"
     CDS             complement(3706..5436)
                     /gene="spxB"
                     /locus_tag="ACG8H8_002291"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP8987340.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5601..6419
                     /locus_tag="ACG8H8_002292"
     CDS             5601..6419
                     /locus_tag="ACG8H8_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP8987341.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6522..6719)
                     /locus_tag="ACG8H8_002293"
     CDS             complement(6522..6719)
                     /locus_tag="ACG8H8_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987342.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6888..7466
                     /locus_tag="ACG8H8_002294"
     CDS             6888..7466
                     /locus_tag="ACG8H8_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP8987343.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7561..7698
                     /locus_tag="ACG8H8_002295"
     CDS             7561..7698
                     /locus_tag="ACG8H8_002295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987344.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2395 a         1408 c         1575 g         2571 t
ORIGIN      
        1 aagctgacca cttctgtcac aacctcttat tttgattaga acttgcgaaa ataaaccggt
       61 taaagtgtgt ctagcgcaag agatttgcta taatagacga taagggtaaa atgtaacgag
      121 aaggggttga aaaatgtgta tcaaattgta acagattctt gttgtgatct gccataccat
      181 gtattagcaa aagaaaaagt tgatttttta tcaatgaatc tccaattgaa tgatcaagag
      241 ttagtagatg acctcggaaa gacattcaat tatgaccgct acatgaatca gctgaaaaat
      301 ggtgcgatgc ctacgacatc acaaatcaat gttggccgat atttagaatt tttccgttct
      361 tatgcggaaa aacagattcc tgttttatat gtggcttttt ccagtggatt aagcggctct
      421 tacagtagtg cgcttcaagc ggttgaattg ctgaaggaag aatatgaaga ccctgagatc
      481 tatgtttttg atacaaaagc tgcaagtatt ggggaagggc ttttagtatt ggaagctgta
      541 agattgaaaa aagaaggtaa aagcatcgaa gaaaccctag agtggctgga aagtaattat
      601 ttgaaagtcc attcatgggt aacagtagac gatctgaaat atctagaacg aggcggtagg
      661 atttccaaag caagtgctac tcttggcagt ctattgagtg taaagcctat tattacggtg
      721 gataaagaag gaaggctgac gaatgttgac aaagttcgag gaagaaacaa gtcgatccag
      781 acaatcgtca atgagacggt aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg
      841 gcttacgctg gggatctaga gagcgcggag aaggcaaaac ggcttttgga agagcagata
      901 aagatgaaag atgtgaagct ttatccctta ggaccaacga tcgccagtca cacaggctat
      961 ggctgtatcg cgattttttc gatgggaatc agtcgataaa agcagtaact cattctgtaa
     1021 aaaagcccag aaattgattg taacaaaaac agcgactctt aaaagataga tgagcatcct
     1081 atctcttaga ggtcgctgtt tttttatttt ttaagctaaa taggccatta tgcccataac
     1141 tcgttcaatt gtttttgaat atctgcattt tccaaaaatt catcatatgt cgtatcagct
     1201 cgatcgatca cacctttatc cgaaacggca atgatgcggt ttgccaatgt ttggataaat
     1261 tgatggtcat gtgacgcaaa taagattgag ccagtaaatg ccatcaagcc gtcattcaat
     1321 gctgtgattg attctaagtc taaatggttc gttggatcat ctaatacaag tacgttagct
     1381 ttcgaaagca tcatttttga aagcatgcaa cggactttct ctccaccaga taaaacattc
     1441 actggtttca atacttcttc accagaaaac aacattcttc ctaagaagct tcttaagaag
     1501 gtgttatcat cttcttcttt acttgcatat tgacgcaacc agtctaaaat cgttagatca
     1561 gtatcgaatt ctttcgttgt atcctttggt aaataagaat tacttgttgt cacaccccaa
     1621 cgtactgttc cagtatctgg cgtaatttca cccatgataa ctttgaatag cgttgttgtt
     1681 gttatatcat tttctgcgac gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata
     1741 tcatctaaaa ttttctttcc atcaatcgtt acacttacat tttctacctg caataagtcg
     1801 tttccaatct cacgttcagg tttgaatcca acgaatggat aacgtctaga agaaggttgg
     1861 atatcgtcta aagtaatttt ttcaagcatt tttttacgtg aagttgcttg ttttgatttt
     1921 gatgcattcg cactaaagcg ggcaataaat tcttggagtt ctttgatttg ttcttctttt
     1981 ttggcatttg cgtttgcttg aagtttagcc gctagttgac tagattccaa ccaaaaatca
     2041 taatttccta cataaagttt gattttacca aaatctagat ccgccatatg tgtacatact
     2101 ttgttcaaaa agtgacggtc atgggataca acgatcacag tattttcaaa attgatcaaa
     2161 aattcttcca gccaattgat cgattggata tccagaccgt ttgttggttc gtctagcaaa
     2221 agaacatcag gtttaccaaa tagtgtttgt gctaataaca cttttacctt ttgtccacca
     2281 gttaattcac tcatttgctg atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa
     2341 acagctgctt ctggttcagc ttcccatcca ttaagctcag caaattctcc ttctaattca
     2401 gctgctcgaa taccatcttc gtcagtgaaa tcctctttca tgtaaatcgc atctttttct
     2461 ttcagcactt catacagacg cttgtgtccc atgatgactg tttcaagtac agtatagtct
     2521 tcatagtcgt aatggttctg tttcaatacg gccatccgtt catctggtcc catagtaacg
     2581 gaacctgtgg aaggctgcag atcccctgat aacactttaa ggaacgttga tttacctgca
     2641 ccatttgcac caatcaagcc atagcagttt ccgggggtaa atttaatatt cacatcatca
     2701 aatagtttgc ggtctgaaaa gtgcaaactc acatcattta cagtaatcaa tttttttcct
     2761 cacttttatc attattagtt ttgacaagtc ttatagaatt atatagggga aaggtaaaag
     2821 attcaaggca atcgaacatt ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat
     2881 aaaagcggag cgaaaatagc tactatagga tttattagtt tgcaacagca taatttttga
     2941 cgttatttat aagagggagt actataaaac tgtaaaagaa atgaaagaag gattgaaaat
     3001 ggatgaaaaa agaaccatcg actggggctc attgattttg ggaattttat ttgtacttgt
     3061 ctcattgtta tcatttcaag accctgtcgg aaatttagtt gcaattgtag tagtctttgc
     3121 gatttttgcc tttattaaag ggatatttga actgtttgta cggaacagat taaaagagct
     3181 aacaggatat aaaggaaaga tgcccttaat aatcgggatc atcgatattc tagttggcgt
     3241 ctttttctta ttcaatattg gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg
     3301 gtttattgct gattcagttt tagcgttatt tactgctgac ttggcaagag gagtaagtga
     3361 aggatattat tggtttacga tcatcgtcaa tattttagga attttattag gaattttctt
     3421 attgtttaat ccaatttctt ctgcgttgac attaagcttt ttagttggat tctattttat
     3481 gttatttggt atcacacata tcgtctatgc atttagataa gtaaaatcaa acaaataaaa
     3541 aagccggctt ccgattacag ttttactgtt gtaattggaa gccggctttt ttatcatgga
     3601 caaatatgca cgaggtgatt tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc
     3661 tatatacaca aattttatct taatactcga agcatagcgc agttgtcaca acctcttttc
     3721 atataattca ttcaatactg gcaaatcatg gacttcatat ttttctttga atgcttggat
     3781 ttcttcttca gaatattttc ttggatctaa atgcagatct tctactggca atgggcgctt
     3841 gttattgatc ttcacatcga ttactacagg acgatctgat tttgcagctg catcaaaggc
     3901 aaatcgaagt tgcgagtatt ctgtgactgt aaagctattt gccccgagcg cttctccaac
     3961 tttaccgaaa tctgcatctt ctaaaaatac accaaatttc ttttgttccg tatcttcttg
     4021 ttctgcttcg atgaaaccaa aagaatcatt cgaaaagaca acattaataa ttggaagctg
     4081 atatttgact tgtgtaatga tatcttgcat gaccatagca aacccgccat caccacttaa
     4141 ggtaaatact tgtttttctg ggtagcttag ttgtgcggca attcctcctg gcacaccatt
     4201 acccatagtt gcgaaccatc cagatgtcgt atgtttttgt ttaccgttca tattcaataa
     4261 acgaatagaa tggatagtcg tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc
     4321 aatacgattg atttctttat agacagcctc tggacgaatc ggttcttcct ctcgatcttc
     4381 aaaagatttc aaccaattta cccatttttc tttattttct tgatttgcta ggtaccagct
     4441 atcagcaggg cgagcatccc ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc
     4501 gaggatcgac aaagatgttt catggcgtcg tccaaatttt gacgcatcga tatcgatttg
     4561 gataaattca gcagttggat taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa
     4621 taagatcaga tcggcttctg ccaatgcttc atttccgggt ttagtagcga cacgtccagc
     4681 aaaaccaaga aagttttcat aatcatcagg aataattcct tttgctagaa cggaagcgac
     4741 aacaggaact gaaaaatgtt ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc
     4801 ttgaccaaca taaagaactg gtttttttgc tttttcaaga taaggcaaag cagctagcaa
     4861 atctttttct tctggtaata tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg
     4921 ttcgtcaatt tcttcaaaac caagatcaac agggatagtc acgatagcca cgcctttatg
     4981 ctcataggcg gctttaatgg cttcatctac tacatgcgga agactttgtg gtgtcatgac
     5041 tgtacgattg tatacgctaa catctgcgaa aataggattt tcatttagtt cctggaaaga
     5101 gttatagttc atggatgtag aagcaacttg tcctaagaac gctactagag gcacatggtc
     5161 catctgtgca tcatacaaac cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa
     5221 gacagcgccg actttccccg ttaattttgc atctgcagcg gctgcgagtg cgccgacttc
     5281 ttcatgacga acttgaatgt agttgatttc ggttttttct tgatatagcg catccataat
     5341 tgaattgaaa gatcctccgg gaatgccgta aatatggtcg ataccccagc tttcaagaac
     5401 ttttaccatt gcgattcctg cattgatttt tgacatggtg tttcactcct ttagcaatga
     5461 tgatacataa attttttgta tctagagtat agaacttttt atcgaaaatc ccaactaatt
     5521 cgtcttttca caaaaatgta gtcatgtttt tgggaatatg ctagagtagt acatagatag
     5581 attcaagaga ggggaatggc atggttgaat ggctattgag acttgaacct tggcagcaag
     5641 cacttgctgg gacagctttt acttatttta tgacagctct tggtgccggg ttggtttttt
     5701 tctttaaaga aatcaaaaaa gaagtgctaa acttgatgtt aggatttgct tctggtgtca
     5761 tgattgctgc aagtttttgg tctttgttag atccggctat tacaaaagca gaagaaaatg
     5821 gagatattgc ttggctagtt gtaagtattg ggtttggttt aggcggtgtg tttttatata
     5881 tggcagataa aacactgccc catatgcatt ttggaccaca acatgaagca gaaggattgc
     5941 ctacccattt aaaacggacg atcttgcttg ttttttctat tacattgcat aacattcctg
     6001 aaggtttagc cgtgggggta gcattcggtg cagcagcaac tgcggataat ccgacagccg
     6061 ctatattagc cgctgtttcg gtggcgctag gtattggtat ccaaaacttc ccagaaggag
     6121 cagctgtatc aatcccgttg cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg
     6181 gacaagcttc tggaatcgtt gaaccaattg ctggagtgat tggagcgcta ctagtaacga
     6241 aagtggagct gctgcttcct tatgcacttg cttttgccgc aggagcaatg atttatgtcg
     6301 tggtggaaga attgatccct gaagcacagc agacattgtc cagcaaacat cattttgcag
     6361 tttttggcgt catgagtgga tttatcatta tgatgatttt ggatgtggca ttaggataaa
     6421 taaaaaaaat atatatgata tatcaaatag ggccgcgaac ttatatcgcg accctatttt
     6481 tttagtaagt tatatcataa tgaagtgaat ggtatcatac attaataata gttttttcta
     6541 tgtgtatttc ctttgatcca gcgaacaagc cagatgataa gtgcgattgg gattagaact
     6601 ttgaccgcaa accataaaag cccgcttagg acactgacaa aaagactgat gacgaagcta
     6661 gctgcaatta gcactaaaag aaccatgaag atatttaaga acatattttt ttgacgcata
     6721 caaaaccctc ctcttgttca ttctttacta tagaaaaagt ataacaggga ttgtttaaaa
     6781 acacctccaa ctgaagactg atttttactt catctactta aatgatcaaa caattgcttt
     6841 ttctctggtt tatcatctaa aatagagcgg aaaaggagga gaatatcatg cctaaaaatc
     6901 gtcttgaagc atttacagat gctgtaatcg cgattatcat gactatttta gttttagaac
     6961 ttcatgaacc taacggagac acgtgggagg cgctcgctgc attaggttac cgatttttta
     7021 tctatattgt cagttttatc attttagcga tttattggaa caatcatcat catttatttc
     7081 aagttgtaca taagataaat ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc
     7141 tttcgctttt tccgtttgca acttcatgga tcagtgagta catcaatagc ctggctccag
     7201 aaatgactta tggttttgtt accttgaccg ctaatattgc gtattatatc ttggcaaaag
     7261 aacttgtcaa agcaaataaa ggccacgaga aagcattgaa ggcgatgttg aataattaca
     7321 agaaatcgta tctatcgatt ggtttgaatg tgttaggaat tgtcttaggg tatttagtag
     7381 cgccagtagc cgtgctgatc gcaaacatcg tgattctagc tgcttggatc atcccaagtc
     7441 gtaccatcga atcacaatat cggtaaaatg atccgtgttt aaaatttaag agaaaaatat
     7501 agaaaaaata aataaacgat caaatatccg agtgatacta gtattcaaag agggatgcag
     7561 atggatcaaa aaagaattca tgtaggactg attactagtt ttgtcacgat gcttatttat
     7621 ttggatcaag gtcatttgac ttggacagta gttggtgtcg taggattttt gacattattc
     7681 acagcaataa aaaaataaac atctttatgt aatctgatgt tctatctatt taagaaaagc
     7741 atgtttacag cagtatgatt ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt
     7801 tagaaaagtg tatcgaaaaa tagatagtta gtttttccac agtggataac ttcgtaagag
     7861 ataaaaataa aaaattagtc aaagaagggc gtatagattt cttttgatag gagatataca
     7921 acagaaatga ataaatatta acgtgtttt
//
LOCUS       DAZKAO010000119         7825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000119 DAZKAO010000000
VERSION     DAZKAO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.120"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG8H8_002296"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG8H8_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP8987345.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="ACG8H8_002297"
     CDS             complement(2203..4389)
                     /locus_tag="ACG8H8_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8987346.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG8H8_002298"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG8H8_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP8987347.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="ACG8H8_002299"
     CDS             complement(4611..5051)
                     /locus_tag="ACG8H8_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP8987348.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="ACG8H8_002300"
     CDS             complement(5125..5664)
                     /locus_tag="ACG8H8_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFP8987349.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="ACG8H8_002301"
     CDS             complement(6279..7745)
                     /locus_tag="ACG8H8_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8987350.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2126 a         1725 c         1370 g         2604 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgtta
//
LOCUS       DAZKAO010000120         7803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000120 DAZKAO010000000
VERSION     DAZKAO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.121"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..2494
                     /locus_tag="ACG8H8_002302"
     CDS             167..2494
                     /locus_tag="ACG8H8_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP8987351.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2491..3405
                     /locus_tag="ACG8H8_002303"
                     /pseudo
     CDS             2491..3405
                     /locus_tag="ACG8H8_002303"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3478..3993
                     /locus_tag="ACG8H8_002304"
     CDS             3478..3993
                     /locus_tag="ACG8H8_002304"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP8987352.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            4130..5452
                     /locus_tag="ACG8H8_002305"
     CDS             4130..5452
                     /locus_tag="ACG8H8_002305"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8987353.1"
                     /translation="MKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKD
                     TAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPF
                     YNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVT
                     GFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKET
                     TFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQ
                     DVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISD
                     SEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKG
                     ILPSTGGMGIIVFILVGTALVGGAVIYFKKTPSRNRSLNYWKQKSYDK"
     gene            5626..6798
                     /locus_tag="ACG8H8_002306"
     CDS             5626..6798
                     /locus_tag="ACG8H8_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8987354.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6960..7634)
                     /locus_tag="ACG8H8_002307"
     CDS             complement(6960..7634)
                     /locus_tag="ACG8H8_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987355.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
BASE COUNT         2658 a         1456 c         1539 g         2150 t
ORIGIN      
        1 tttttattta aaaataaaat ctttgtatta aaggacaaga ctaaaataac cttagaataa
       61 aaagagaaaa aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag
      121 aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa
      181 gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc
      241 tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt
      301 cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa
      361 ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac
      421 caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc
      481 gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg
      541 tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc
      601 agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat
      661 gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg
      721 tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc
      781 aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa
      841 tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg
      901 tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc
      961 gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca
     1021 agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg
     1081 tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa
     1141 agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt
     1201 cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac
     1261 tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac
     1321 ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt
     1381 tacagctgtt tcagcgacag aaacaaagaa ccaatcagca atgagtgttt tagctagttt
     1441 agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat
     1501 cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga
     1561 tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc
     1621 ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc
     1681 gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg
     1741 gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat
     1801 cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg
     1861 cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg
     1921 tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga
     1981 tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag
     2041 cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac
     2101 atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt
     2161 taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt
     2221 cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca
     2281 aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac
     2341 agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg
     2401 tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct tttattttat
     2461 ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat
     2521 atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca
     2581 gctagagaat cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg
     2641 ccaagtatca gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg
     2701 ttgaacggcg ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca
     2761 gaaggaagta gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa
     2821 atactggcag aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt
     2881 gcatcggaca aagatgagca aggtcgtgac gctgtttacc gatttgcgga aataaagact
     2941 tcagattaag taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca
     3001 tctaatcata aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct
     3061 gcactcacat taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa
     3121 gttccttatc tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg
     3181 atcaaagata cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt
     3241 ggtggagaca agattcatac atttcacaca caaaaaacaa aacatggttt tgtcttatca
     3301 gattctggaa aagatcttag ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg
     3361 acattgaaag aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac
     3421 aatcaaacag tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg
     3481 gatcggcaaa cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa
     3541 tacttagcgc aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg
     3601 atttttactt ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac
     3661 gaactagtgg agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt
     3721 gaagttgaaa aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa
     3781 ccaaaagcaa atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca
     3841 tcaaatgaaa aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc
     3901 tcaattttag ggttgttgct agtcctttca acagggacag cttggtttta taaaaaacaa
     3961 caaaaaggaa acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt
     4021 ttttaggaac tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt
     4081 ctaggccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac
     4141 ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc
     4201 gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga
     4261 cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt
     4321 agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga
     4381 aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa
     4441 cccgaattca tcagaccctg acaaaccttt agacacgatt cacctatatg ctaaaaatgt
     4501 catcaaagaa agcaatatca aaatccagaa agctgggaac ttttcagggg tagataagtt
     4561 agcaggggcg caattccaga tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga
     4621 aaacactggt gtcgctctct atgatcctgc tgagtcagcg ttgacattca caactgatac
     4681 tgaaggatcc atcagtgtca atggattaat agacggcaat tatactttag ttgaaacaaa
     4741 agcaccagat ggttatcaat taaccaatcc acaaaaagaa actactttta tagtaaaaaa
     4801 cggaaaattg caacaaacaa ccggtatcat ccatggcgca gatgaagcca atcttgggaa
     4861 tccaaacggt gtagtcaatc gggaaaatac aactacggat aacacgatta ctgtagaaaa
     4921 tgtccaaaac tacggtgatt tccacttcat caaacaagat gtgaacacgc aagaaaaatt
     4981 agccgatgca gaattttcta ttctaagagc aaaagatcca actgttaaat taatgaaaaa
     5041 aatagcaaat cttgcctcag gagaataccc atatgcttgg tcagatgaag tcacaggttc
     5101 tgactgggaa gaagtgaaat tgatttctga ttcagaaggg aaattttcaa tcgaagggct
     5161 gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca gaaggctacg cattacctaa
     5221 gaatgaagct gcgtacactg aatttacagt agatgatgat ccaacaactt ctgatgaaac
     5281 aacgatcgat aaccaaccta aaggaatctt gccttcaact ggtggtatgg ggatcatcgt
     5341 attcatcttg gtaggtactg ctttagttgg aggagcagtg atctacttca aaaaaacgcc
     5401 atcaagaaac agaagcttaa actattggaa acaaaaatca tacgataaat aaacgatagt
     5461 gattcaaatg gggagaaagg ttgtgacaag tggtcggtct cttggtattc gctcaacaaa
     5521 cggcaaaata ggaggaacta ttttgccgtt tgttgagcga atactcaggt caaggcgctg
     5581 atttcacaac ctcttcatat acatattaga aaataggtgg acatgatgaa taaaagcaga
     5641 ataagaatac tggacatctt catggcaatc attttagtcg ttgggatcgg gatcttttct
     5701 tatccttttg tagaagattc actgaatgat tttcttgccc agcaaatgat catccattat
     5761 caaaagcaag catcgaataa gaattcagag gaaataaaaa aacagcagga aaaaatgaca
     5821 aagaaaaacc aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa
     5881 gcagttgatg caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg
     5941 cttggagtca tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg
     6001 gaaatatttt tgcaaaaagg aacgtcatta ttggaagaaa gttcttatcc gactggcggg
     6061 aaaaacaccc atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca
     6121 gatttgccta agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg
     6181 gcgtatcaag tagaaaaaat ccaagtggtc ttacctgatg aagtagattc tttgggtatc
     6241 caaaaaggca gagatctcgt gactttgctt acctgtactc cttatatggt caatacacat
     6301 cgactgcttg tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc
     6361 caaggaattg atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt
     6421 ggatcaatcg gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag
     6481 cgaaactatc ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg
     6541 gtcttttctg taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt
     6601 gtgaaagctt ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat
     6661 cgcttgcaac atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag
     6721 ttttcaatga aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat
     6781 ttgattgaga aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa
     6841 aaagagagaa gctgacgaaa tggacgtgta aactgctatt tttggtttac tatctggaac
     6901 gccagcttct atttttttat gtttcagaag aattttatta ttatagtata acttttgaat
     6961 tagtttccat aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg
     7021 gcaggggtat acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg
     7081 acatagacag tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaggcc
     7141 tgttcagctg ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt
     7201 tgcgcttgtt ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct
     7261 tcttttgctt tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt
     7321 tcaggtagcg cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct
     7381 tcagcttgtg acaaagcaga ttccgcttct gtcacttttt tttctagtgc agctttttcc
     7441 gctttttgtt tctcttcttc ttttttcttt tcaatttgtt cttgttcttt cgatgaatct
     7501 gccgtacttt cggagatcgt ggtactgctg ctggaagcct gagctgaact tgtgctttga
     7561 gtagtagacg tagtagcacc acacccactc aacaaacttc ctgcgaccaa aaacaatgca
     7621 actaattttt tcactaaatt tcctccttaa gtaaaaaaca accgttttca ataaatgtcc
     7681 ttttcaattg tttacttact tgttataata attgtgttat gaatagtgat tgaggaaact
     7741 taatctaatc ccgttttgat acacgggatt ttttgctata gttctgttat taagtatgca
     7801 ata
//
LOCUS       DAZKAO010000121         7720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000121 DAZKAO010000000
VERSION     DAZKAO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.122"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG8H8_002308"
     CDS             complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG8H8_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP8987356.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG8H8_002309"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG8H8_002309"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP8987357.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG8H8_002310"
     CDS             complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG8H8_002310"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP8987358.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="ACG8H8_002311"
     CDS             5737..7038
                     /locus_tag="ACG8H8_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP8987359.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="ACG8H8_002312"
     CDS             complement(7107..7706)
                     /locus_tag="ACG8H8_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8987360.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2285 a         1587 c         1358 g         2490 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc
//
LOCUS       DAZKAO010000122         7702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000122 DAZKAO010000000
VERSION     DAZKAO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.123"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1345)
                     /locus_tag="ACG8H8_002313"
     CDS             complement(203..1345)
                     /locus_tag="ACG8H8_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987361.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1350..1535)
                     /locus_tag="ACG8H8_002314"
     CDS             complement(1350..1535)
                     /locus_tag="ACG8H8_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP8987362.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1889..2134)
                     /locus_tag="ACG8H8_002315"
     CDS             complement(1889..2134)
                     /locus_tag="ACG8H8_002315"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987363.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2128..2529)
                     /locus_tag="ACG8H8_002316"
     CDS             complement(2128..2529)
                     /locus_tag="ACG8H8_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8987364.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="ACG8H8_002317"
     CDS             complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="ACG8H8_002317"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP8987365.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4547..6313)
                     /locus_tag="ACG8H8_002318"
     CDS             complement(4547..6313)
                     /locus_tag="ACG8H8_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP8987366.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6326..7120)
                     /locus_tag="ACG8H8_002319"
     CDS             complement(6326..7120)
                     /locus_tag="ACG8H8_002319"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8987367.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(7130..>7702)
                     /locus_tag="ACG8H8_002320"
     CDS             complement(7130..>7702)
                     /locus_tag="ACG8H8_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8987368.1"
                     /translation="YNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMF
                     FSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQRED
                     GTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICF
                     LIVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2456 a         1497 c          957 g         2792 t
ORIGIN      
        1 aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac atggtttatt ttttacactg
       61 ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg tccattttcg ataccgacag
      121 ctagcttata acccaaggta tatcaacata aaaaaataaa aataggctat gacagcagcg
      181 tgacagcaaa aaagatggaa agttaaatat caaacctcgt aatttcattt gcgagtgctt
      241 gatttgtttc tggatataag tgtccatatt tgtccaaagt agttttaata gaggcatgac
      301 ctagacgctt agatattgca tagatatttt tattatgttc aattaaaaag gcaacatgcg
      361 agtgtcttaa tccatgcata tgaattggtt ttactcctgc aatttcagct agttttttta
      421 ttctgcttaa aatagttctt ggagatggcg gtaatccatc aaaagaaaaa accaattcta
      481 tatttttccc aattgattgt tgagtttttt tccatgtttt taaatcttca acggtttgtc
      541 tgtctaacga aattatccga atacttgatg tattttttgt ttcagcaaat tcataatctt
      601 gtcttgtacg gatgtaaatt gacttattta cgctacatct gccatttatt aagtcaaagt
      661 ctttccattg taacgctaag agctcctcac tccttacacc tgtaacaaaa ataaaacgca
      721 tcaaacgttt ataaaattct tcataaaaat ccccgtgata aacactatta tatactttct
      781 taaattcatc aactgtccaa aattctactt gaggacgctg agtttttatt ttacctatag
      841 tatctacagg attttcttca ataatttcaa gtacaattgc ccttttaaaa actactctca
      901 acttattgaa tatctgttta atataatttg gtgataatgg ttgctcaatg ccctttttta
      961 caacatggat tttcgataaa aactgttgaa attcctgtat atgaattggt cgaatcttta
     1021 caacctgcat ttctccaaaa aaatccaatg cacgttgtaa aatatgatct gttttcatat
     1081 atgttttttc tacagttccc aacttatacc aaggaaaaaa atactctcga taaaaatcat
     1141 gaaacgttat tccttgcaga taaattcctt gtgaatcaga aaaactttgt tttaactctt
     1201 cgagtcccct tctagcttct ccttgagttt tgaagcctct tctggtcact tgaattcttt
     1261 ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca agtgcctctt tttctgtctt
     1321 tataaacatt cgcaattttc ctcattttac tattcctccc aagaaatatg gagaacttct
     1381 tctaaaattg aaataggaat tctttttatt tttgaattac gataaaaaaa gtatcctttc
     1441 tcaactaaaa gttctttaca ttccttgaag attttattag catagtactg agaaaaacct
     1501 agattaatca aatcttttcg agtagctgtt ttcattatag caccttcctt actttttttc
     1561 atttatttct atcagctgct tttgtcaaaa gcacatagga atttcacctc tccccaagtt
     1621 tacggcgagc cttacggaag tatcattatc ccaactttaa atcatcgctt tttattgact
     1681 ataaaaatta ttttagaata tttatcgctc cttctttaca gcaagaaatc atggcgcaac
     1741 taaaatatgc tccttaaagt gttaacgtac tgatagaatc tatttaattt tcaatgaaca
     1801 ataggttttc tacctaaaag aatctactaa ttccttaact attagaagat tcttttaaac
     1861 agaaaaagtg gaaaggggat ttttttaatt agttaatttt aaattttaag atcgcttcta
     1921 ttaatttaat ttctagctga cttatcatat attcatctac ataggtaacg aattgaccta
     1981 cctcgttaaa tccatttgtc aaacataact tagccatata agaatgataa tgtttgagta
     2041 tcatcttgat cgaaattgga tctccttcta gagcagataa gataatggat agtggaacta
     2101 ataggtactg ccgcttttta gatttatcta ccataggtca taccccattt tcaattgttc
     2161 aactgccttc tttcgttgat accagatacc acttgtgctc atattgagaa ttttaccgat
     2221 ttcagcatca ttaaaacctg caaaataata aagtaggata atctttcttc ttatctccga
     2281 taactgatca attgctgctg ataacttttg atctgaaatt aatatttcca ttccaaataa
     2341 gaaatagaca tcttgttcat caaaatgagt ttgggaatac ctgccttcat ttgaatctgc
     2401 caatggttcc tcattatcct ttatgttagc gtttcgtttt cgaatagact tagcttcgtt
     2461 tcgtaatact tttttacagt aggcatcaaa ttggtgttgt atagatatgt cattgtaatg
     2521 ttgttgcatg ataatatgct cctttttccc cctttccact aatacaacaa tcagaaagcc
     2581 cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct ttaaacagaa
     2641 aatctttcta taaaaaacaa aaaaaaacga ttaggctagg ctaatcgttt tttgaacatt
     2701 cttaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc atcgtttctg
     2761 tagatagtaa gcaatcattt taggttttct atctctagta aagattcctt tcttatttcc
     2821 ttgcactctt aaaatccctt gacttgttgc aaaatctgca aaattccaca cttgttcacc
     2881 aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag ccacttgata
     2941 ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat ctgctccata
     3001 ttctgtaaac attattggcg tatcaggaca tctttccatc catccatcta gctctttttt
     3061 caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat aataacgatt
     3121 taaacataaa acatcgacaa tatctcctac cttacaaaca tctggcgttg attttagata
     3181 agtaacgatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt caactaaagg
     3241 ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa tagaccacat
     3301 tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg ttttatgaga
     3361 attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat ctaataaagt
     3421 ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta caataccttc
     3481 tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc taaaagaatt
     3541 agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat tcaaaccacg
     3601 tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag gtttattatt
     3661 aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt ctgtataggt
     3721 atcgatcact ttattatcat caattaaact aattttcata ctatataaat aagcgtctag
     3781 aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt cagcttcagc
     3841 tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc attctgtatc
     3901 gatattgaat tctacacttg catccaaatc agttattctt gtattaatag taatatcact
     3961 tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg cataattgaa
     4021 gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta ctgtttcctt
     4081 cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa ctgtaattcg
     4141 attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa acccaccttt
     4201 gtgttgagta ataaactttc cattcaaaaa aacttttgct tcatgtgttg cagatccaaa
     4261 acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt tttcatacca
     4321 cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaggg
     4381 aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt cttcctgtaa
     4441 gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta cttgcctcgt
     4501 ctctgtcata atgggataca acatataaac cactcctatc taaatattaa ataaatgtat
     4561 cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa agacccatta
     4621 gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta atataagtct
     4681 ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa attgttggaa
     4741 tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag acaattcgtt
     4801 gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt ttagttaaat
     4861 ttttaattgg tctcttagca gcatatattc cagctatatt tttaattttt tcttcttgcg
     4921 ataatccttt caattcttct tcaggtgaaa tgtatttttc aacttcaaat ccttttgctg
     4981 acaaaatctg tttcaataaa tcaatggctt gaggtctatt tccagcaact acttcaccaa
     5041 acccaccttt taccccatgt acatctacta gtaaaatcct tttatatttt tctggtacaa
     5101 taggccaaat atcttgttta tctttaacta atgtaattgc ttggtcagcc acctctttaa
     5161 aaattgattt attttcaatt aaaccaattt tttccagagc ttcttgctta ggtaataaaa
     5221 tttgttcttt tgcttttttg tgtaatccca atttagcttt taaccctaaa attcttttta
     5281 aggcatctaa taaccgatca tcagtgataa ttcctttacg atagccttcc atcatataat
     5341 taaagtcttc atctggatca ttaaaaaata gaaatagatc acatccagct gcaatagaag
     5401 tgggcaataa ttctgatcgt ttcattgccc cagtcattcc taacatatga ctagcatcag
     5461 taactactaa tccattaaat ccgagttttc ctctaagtaa gtcagtgatt aattcttttg
     5521 ataaagttgc aggaagtaaa tcttcatctt tcagatcagg attaaaatgt tttgaataag
     5581 ctggcatatg gatatgacct gccataattg aaggtaatcc agcttctatt aagcctgagt
     5641 atacttttcc aaaagaatta tcccattctt ccgtacctaa actattaata ctaaaagata
     5701 gatgctgatc tcgttcgtca ataccgtcac cagggaaatg tttagctgca ggcaaaattc
     5761 cactctcctg aatacctttc atataagcta aactaagttc taaaacttta tcagggtttt
     5821 gtccaaaaga tcgactagaa atgataggat ttctccaatt tctattaata tctacaatag
     5881 gagcaaaact ccagttacat ccaatagccg atgcttcaat cccagaaact ctccctaatt
     5941 cataagcata tttcgcatca tttgttgcac caatttttac ttctacacca acttcagtac
     6001 catctgtaca agctccgtta ccacctgctt ccgtattagc agcaattaat agaggaattt
     6061 tactttctgt ttgtaagata tagttttgat catatacctc atcagcagtt cctggattat
     6121 atcgtacagc tccaatatgg tactgattga ccatattact caagtaattt ttatcacgac
     6181 tagatcccat attaacaaac aattgaccta ttttttcttc caaagtcata gaatcaatcg
     6241 tctcttcaat ccaagaaata gcatcttcat ctaaattata tggttttttt cgtaaatcta
     6301 cttttatagt cattttcttt ctcctctact tattagtaag atttttccaa aaagtatcta
     6361 tatatttttg ttcaatatct acataatctc ccgataaaca tttaaataaa acatcagttc
     6421 ttaagttttc ccatgattct ttttctttat tgaccaaaat aggataaaag aaaactttat
     6481 ttttatttgc agcttctaga tcaccaggag aatcaccaat cattaatatc ttatccaaat
     6541 cacaaccatt ttcaattaca cttcgaataa tatcttcttt ttttccttta tcttgacaac
     6601 acagttcatc aactaatgat aataattcat gtctttccca ttcttcttga acagcttcct
     6661 tattagctga gcttacaata attatcttcc catatttttt cagagctttt atagtatctc
     6721 tagcacttaa aaagggttta tctaatcctt ttgcttgact aatctgttga ttgacttctt
     6781 ccgaccattt taaagctttt aataaatcct ctgactgaga atattctatt tccctcttca
     6841 aagatggatt agacaattct tttgttagag atacccagtg ctttaatcga ttaatcgatg
     6901 ttacatcatt tttttctaat gttttaacta aacctaaaaa tctgtttatt ccacgagtct
     6961 ctgaatataa attaatatta ttccaatcct gtaggaattt ttctctatga tgaacatcaa
     7021 aaatctctgc agcaattggt ccgaaaaaga gattgtgttt ataagtcatc gtatccataa
     7081 cacagccatc tgaatcaata caaaatatcc aattattcat atttctctcc tatttatgtt
     7141 tttgttttaa aagctcgcaa ttttctaaga ctttatcttt agataatgga taaaggaaaa
     7201 ataaaattaa tcctacaatc agaaaacaaa ttgatggaat taatgttgaa gcgttatata
     7261 acttttctaa aacatctccc gtttgtacat ttgctaaaga gtcatagcct ataatagaaa
     7321 gagtccaacc tccgacgcct ccagctagag cttgacctat ttttctagca aaagaatata
     7381 ccgcatatac ggtcccatct tctctttgat cagactttac ttcttgatca tcaataacat
     7441 cagtaatatt tgcccaaata acagtattaa acacacccaa acccatatag ccaaccgaac
     7501 taagaattaa gaatacatac atattactag cttttatcaa aaataagatc aaatatacta
     7561 ttgctgaaaa gaacattcct actgatgcca gttctttttt tccatatttt ttacttaaaa
     7621 acaaagttaa aggcgaaaca actattaaaa ccaaaatagg attgataaaa ttcattaaaa
     7681 ctattccctg tgcatcatta ta
//
LOCUS       DAZKAO010000123         7652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000123 DAZKAO010000000
VERSION     DAZKAO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.124"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1599)
                     /locus_tag="ACG8H8_002321"
     CDS             complement(139..1599)
                     /locus_tag="ACG8H8_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987369.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(1807..2733)
                     /gene="glsA"
                     /locus_tag="ACG8H8_002322"
     CDS             complement(1807..2733)
                     /gene="glsA"
                     /locus_tag="ACG8H8_002322"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP8987370.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2756..4183)
                     /locus_tag="ACG8H8_002323"
     CDS             complement(2756..4183)
                     /locus_tag="ACG8H8_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8987371.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(4214..5428)
                     /locus_tag="ACG8H8_002324"
     CDS             complement(4214..5428)
                     /locus_tag="ACG8H8_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP8987372.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5514..5741)
                     /locus_tag="ACG8H8_002325"
     CDS             complement(5514..5741)
                     /locus_tag="ACG8H8_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987373.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(5769..6011)
                     /locus_tag="ACG8H8_002326"
     CDS             complement(5769..6011)
                     /locus_tag="ACG8H8_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8987374.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(6043..6600)
                     /locus_tag="ACG8H8_002327"
     CDS             complement(6043..6600)
                     /locus_tag="ACG8H8_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8987375.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6613..6801)
                     /locus_tag="ACG8H8_002328"
     CDS             complement(6613..6801)
                     /locus_tag="ACG8H8_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP8987376.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6814..7380)
                     /gene="amaP"
                     /locus_tag="ACG8H8_002329"
     CDS             complement(6814..7380)
                     /gene="amaP"
                     /locus_tag="ACG8H8_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP8987377.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         2333 a         1568 c         1257 g         2494 t
ORIGIN      
        1 tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc
       61 gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt
      121 ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat
      181 atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt
      241 ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag
      301 acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc
      361 ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga
      421 aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact
      481 tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt
      541 ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag
      601 atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa
      661 aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg
      721 taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa
      781 atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa
      841 aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg
      901 acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc
      961 aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc
     1021 atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca
     1081 agcttcttct acatacggat ttttattttc atagccttcc caattgacat aacgtactaa
     1141 agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata
     1201 atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga
     1261 aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa
     1321 aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc
     1381 atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc
     1441 tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga
     1501 aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac
     1561 ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt
     1621 tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag
     1681 aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca
     1741 atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa
     1801 gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat
     1861 accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg
     1921 atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg
     1981 gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat
     2041 tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc
     2101 cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa
     2161 ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta
     2221 agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa
     2281 tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc
     2341 atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg
     2401 attcattgga tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc
     2461 ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa
     2521 taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc
     2581 ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac
     2641 ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt
     2701 tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc attttttatt
     2761 tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga
     2821 taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag
     2881 caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa
     2941 cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt
     3001 taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa
     3061 cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga
     3121 cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt
     3181 gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg
     3241 cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa
     3301 tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta
     3361 acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg
     3421 tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg
     3481 gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca
     3541 tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa
     3601 tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc
     3661 ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa
     3721 tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca
     3781 ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg
     3841 ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt
     3901 ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga
     3961 caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga
     4021 taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga
     4081 atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga
     4141 acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct
     4201 atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt
     4261 gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg
     4321 tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt
     4381 accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg
     4441 tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt
     4501 tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca
     4561 tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg
     4621 atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc
     4681 aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt
     4741 catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg
     4801 taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc
     4861 tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg
     4921 ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt
     4981 ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta
     5041 aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca
     5101 gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc
     5161 ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc
     5221 gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga
     5281 gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa
     5341 aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat
     5401 ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat
     5461 aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa
     5521 agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa
     5581 agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt
     5641 tttaaagata ttttttcata aggaataaag aatacttcat ataaaatata taaaaaagaa
     5701 ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca tttcaacata agtttttctt
     5761 caatttgctt ataatttacg tgcaaagaat gaaacgacta atactaaaat gattgcaccg
     5821 atcaatgatg gaatgatagc cattcctgca attgttggtc cccaagttcc tagtaaagct
     5881 tgtccaattg ctgaacctac taaaccagca atgatgtttg cgatgatccc cattgatgat
     5941 cctttattcg tgattgctcc tgcaattgct ccaatgatac cgccgacgat taatgcccaa
     6001 ataaatccca ttttaaattc ctcctaactg acttcttact tcttattgta cacgtggttc
     6061 gttattttct ttgacttttt gtgcaccttt gcctacagca gattttgctt tatctgtttg
     6121 gcgtgaagca aattgtcctg tggattttgc tgcttctgtt actttgtctt gtactgtttc
     6181 ttgatctttt tggtattctt cttgtgtttt gatgtctgca acattcacat tcacttcgat
     6241 aacatctaaa tgtgtcatat tgtttacttc agtactgatc aattctttga tttggttgta
     6301 aatatcttcg atgtctttac cgtattctac gatcacatct agatccacag ctacttgttt
     6361 cttacctact tctgtattga tacctgctgt tacgttatct gtgttgacta atttttcagc
     6421 catatttgag aaaaatccgc catctaatgt caatagacca tctacatctt tcaatgcata
     6481 accgatgatt ttttggatga ctttgtcttc ataagtcaat tcaccattca tcatgcggtt
     6541 ttctcctttt tcatttgcca aaactgatcc atttgttgta ttcatgtttt tgttttccat
     6601 ctttgtttcc tcctatgatt taaaaaattg atcaactatt cctgactgtt tcaaataaaa
     6661 accgattcct gcgccaattg ctgtcatgat tatgatcaac aatgtcttga aaaatccaat
     6721 cgttaaccat aaaacagcta gcagtagtcc aagaccccca aagataatag ggagcttgta
     6781 tgctgaaaat agttctttca ttatttggcc ctcctattcc actcgagagt gcttgtagtc
     6841 tcttgtatct tcttgttctt cataaccgct gtatgtgact gccactttga ccgtttcttt
     6901 tgttccgagt actttagtta cttcttcttc gatatcttgc atcaatacac ctgttttccc
     6961 tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg atatggattc gatttttcgt
     7021 tgaacgtatc cgtactttag gtgaatgaat gaactcttct tcgtgtaaat gggaacgtac
     7081 caacccttcg atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt tcataacgaa
     7141 agttcctctt gactttggat agaaaagaac gactagcaag atgataatag caagaacggc
     7201 aaatactaca gctgcccaga aaatatattg ttgcatgtag taattcgtta aagtaaagaa
     7261 gcgaaatcgt tccaattgtt caggcataat atacggcgca ttgatcaacg caatcgctag
     7321 aaatactgaa agtagcagta aactcatgat aacaccgatt atttttaacc ccttattcat
     7381 tttccacctc ctataaactt atttttaccc tgtaaataac tctaccgaca atcacagtat
     7441 agatagcgaa cagttagcat agcatcagta aataaagcgc taccaaacat tgctggatca
     7501 taacggcgtt ttattgccag tctgatactg aaataacaat tgcttactta ttctctattt
     7561 acttttgatt gctggctata cttaaaggat attattcaag attaaaaatt gcaaagatta
     7621 tgctcagtta ttattacaag attctattta tt
//
LOCUS       DAZKAO010000124         7627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000124 DAZKAO010000000
VERSION     DAZKAO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.125"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..714)
                     /locus_tag="ACG8H8_002330"
                     /pseudo
     CDS             complement(16..714)
                     /locus_tag="ACG8H8_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            793..969
                     /locus_tag="ACG8H8_002331"
                     /pseudo
     CDS             793..969
                     /locus_tag="ACG8H8_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="ACG8H8_002332"
     CDS             complement(1145..1714)
                     /locus_tag="ACG8H8_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8987378.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="ACG8H8_002333"
     CDS             complement(1788..2111)
                     /locus_tag="ACG8H8_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987379.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="ACG8H8_002334"
     CDS             complement(2265..3077)
                     /locus_tag="ACG8H8_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987380.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3404..4279
                     /locus_tag="ACG8H8_002335"
     CDS             3404..4279
                     /locus_tag="ACG8H8_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8987381.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4293..4616
                     /locus_tag="ACG8H8_002336"
     CDS             4293..4616
                     /locus_tag="ACG8H8_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987382.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4613..5365
                     /locus_tag="ACG8H8_002337"
     CDS             4613..5365
                     /locus_tag="ACG8H8_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP8987383.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="ACG8H8_002338"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="ACG8H8_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2470 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacct
//
LOCUS       DAZKAO010000125         7600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000125 DAZKAO010000000
VERSION     DAZKAO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.126"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8H8_002339"
     CDS             complement(138..428)
                     /locus_tag="ACG8H8_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987384.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8H8_002340"
     CDS             complement(546..806)
                     /locus_tag="ACG8H8_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987385.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8H8_002341"
     CDS             complement(943..1356)
                     /locus_tag="ACG8H8_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP8987386.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG8H8_002342"
     CDS             complement(1812..2297)
                     /locus_tag="ACG8H8_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987387.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG8H8_002343"
     CDS             complement(2436..2651)
                     /locus_tag="ACG8H8_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP8987388.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG8H8_002344"
     CDS             2875..3675
                     /locus_tag="ACG8H8_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8987389.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG8H8_002345"
     CDS             3694..5562
                     /locus_tag="ACG8H8_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP8987390.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG8H8_002346"
     CDS             5555..6046
                     /locus_tag="ACG8H8_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP8987391.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG8H8_002347"
     CDS             6034..6588
                     /locus_tag="ACG8H8_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP8987392.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACG8H8_002348"
     CDS             6606..>7600
                     /locus_tag="ACG8H8_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP8987393.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZKAO010000126         7551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000126 DAZKAO010000000
VERSION     DAZKAO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.127"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG8H8_002349"
     CDS             complement(29..1261)
                     /locus_tag="ACG8H8_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP8987394.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG8H8_002350"
     CDS             1516..2085
                     /locus_tag="ACG8H8_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8987395.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG8H8_002351"
     CDS             complement(2263..2703)
                     /locus_tag="ACG8H8_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP8987396.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG8H8_002352"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG8H8_002352"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP8987397.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG8H8_002353"
     CDS             3657..4580
                     /locus_tag="ACG8H8_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP8987398.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG8H8_002354"
     CDS             complement(4805..5044)
                     /locus_tag="ACG8H8_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987399.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG8H8_002355"
     CDS             complement(5049..5468)
                     /locus_tag="ACG8H8_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP8987400.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG8H8_002356"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG8H8_002356"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFP8987401.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..>7551)
                     /locus_tag="ACG8H8_002357"
     CDS             complement(7040..>7551)
                     /locus_tag="ACG8H8_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8987402.1"
                     /translation="KGAIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEK
                     LREIFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDK
                     SLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEET
                     ISAFIQFCK"
BASE COUNT         2429 a         1395 c         1312 g         2415 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta g
//
LOCUS       DAZKAO010000127         7323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000127 DAZKAO010000000
VERSION     DAZKAO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.128"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..893)
                     /locus_tag="ACG8H8_002358"
     CDS             complement(<1..893)
                     /locus_tag="ACG8H8_002358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8987403.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1270..1908)
                     /locus_tag="ACG8H8_002359"
     CDS             complement(1270..1908)
                     /locus_tag="ACG8H8_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987404.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1912..3894)
                     /locus_tag="ACG8H8_002360"
                     /pseudo
     CDS             complement(1912..3894)
                     /locus_tag="ACG8H8_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4074..4739
                     /locus_tag="ACG8H8_002361"
     CDS             4074..4739
                     /locus_tag="ACG8H8_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987405.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5136..7169)
                     /gene="brxL"
                     /locus_tag="ACG8H8_002362"
     CDS             complement(5136..7169)
                     /gene="brxL"
                     /locus_tag="ACG8H8_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFP8987406.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2353 a         1425 c         1086 g         2459 t
ORIGIN      
        1 ctagaatctg aagtgaatgt actcaaaaca ttttcaaagt ctttgtcttt gatttttaca
       61 ttagcgtctt tcaatacttc gccaattact ttattctgga aggtactgtc acttaatttt
      121 gtgtctgttg caatttcttt caattctttt ttgtatttat ccatgtcgtt gcctttgttt
      181 tggtttttca ccattttaac cacataatat tccgtagtat aagttgaagc attggtagaa
      241 gtgatcacat cagaaacttg accatctttc aacttaaagg ctgcttcttt gacttctgcc
      301 ggaaccgttg tagtagtgga atcaaatttc acttttccgc catcttcttt tgtcgtagta
      361 tcagttgact tgtctttggc taatttagag aaatcgtctc ccttatcagc agctttcttc
      421 acatcttttg catcatcttc acttgctact tggataattt gagcttccac ttctggatgg
      481 aaagattccc aagccgtttt taaatcttca tcggtcaatt tgatatgttt ctttagacct
      541 tcttgaaagg ctagactttg tttgatactg tctttgaatg tttgttctgt gtaacctgca
      601 gatttcaatt gtgaatcaaa tgaatcaccc aattgtttct ttgtttgatc atattgggaa
      661 tcgatttctt tggttgatac gtcatctcca tatttatctt caaatacttg ataaacgatc
      721 aactgttgca cagcggaatc tgctccttgc atttttttca agctatccat tacttcttgt
      781 tcgctgattg taccgccttt ataactaacg gctgtttttt caccggcatt cgtacagcct
      841 gacaataagc cagtgcctag tacgatggca agtgctaaaa atcttttttt catttatcaa
      901 ttctcctact tttcgttttt tgcgaattgc taaaaacaaa ggtgttcaag atgaaaatca
      961 tcaagaacac ctttactaat ctgctcgata cgtcctgaat agatataagg ctacacaatc
     1021 cagaaagttc gctcagcaga ataaatgtca agtagcacat cacaccttaa tatactactt
     1081 cttttgttac cgtttttcaa gtctatcttg cgttccgtat ttaacgtaac taattcttag
     1141 aacaaatgtt atcgattttg tttttcttct tccgctgttg ggatattcaa atcagacact
     1201 tttatgttcg attttccaga catttttttg ttgtttttat tagaaaaagt ttgttttttc
     1261 atttgttttc taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt
     1321 gaccacgata actaacgtac cttgggaagc ctgttgcgat aagatttcaa cgacttgctc
     1381 cttatttcgt ttatctaatg atccagttgg ttcatcggct aaaatgattt tgctattctt
     1441 caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg
     1501 cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt
     1561 atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc
     1621 atattcaatc aacccaaaat cttgaaaaag aaaagagagg gcatttcttt ttatcaagcg
     1681 cttctctctt ccagttaaat gttttgtttc ttgattaaat aaataaaatt gccctgaact
     1741 gaattgatca ttcaatccta atatattcaa taaagtactt ttccctgctc cgctctctcc
     1801 aactacagtc acaaaatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt
     1861 gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc
     1921 attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat
     1981 aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag
     2041 aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt
     2101 atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta
     2161 gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa
     2221 aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt
     2281 ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc
     2341 aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc
     2401 gctgagaata tttctttccc aatacgtact gtttttcaat gtcagtattt caacaaccgg
     2461 ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat
     2521 aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag
     2581 gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc
     2641 tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata
     2701 attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga
     2761 tacattgact aatatacttc cgtttttatt caaagtttca tataattcct gatcggctgc
     2821 tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata
     2881 aaatacatga tagtcctcac cagcgatttc ttttggtgtt tcatgggaaa cagctaactg
     2941 tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa
     3001 caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc
     3061 gattttttca actaataaaa gagaaatcaa agagataccc tatattccgc tgaacaagaa
     3121 aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc
     3181 aatgattcct gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa
     3241 tacttgatgt aatgaaaagc cattattcaa caataaactg atttctttat tcatcaataa
     3301 gaaaaaggat gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata
     3361 attatatacg tctgtacttg aaataaccga atacgaatag ctattttcaa agtcttcaaa
     3421 aatattagtg gcataatcag tgtgaaatgc ttgattgaat cgttccacaa atagtaaaat
     3481 gaaccgttca taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt
     3541 tattttgatc gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt
     3601 ctcgtgagaa gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat
     3661 cttgaagttc caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga
     3721 aagctcagtt tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg
     3781 cccaataaaa tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc
     3841 atttgtccga tagagtacct gtttatttat aataaccaag gcaaaaccac tcatgatacc
     3901 ggtcactatg attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta
     3961 cttaaatgat tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaaat
     4021 gtataggtca tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca
     4081 tcggagaaaa aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg
     4141 aaaaaatcgg tgtttccgat aaaacaatct ctagttggga aaattctcgt acaatgccag
     4201 accttgaaat gctgtcactt ttacatcaga ctttaggtct gccgattgat tttccttcta
     4261 ttgaatcagc taccgaatca atagaaccaa cattgccgga tgaacctctt gttatcaaga
     4321 aatcttattc atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg
     4381 tcttttgttt ggtctatctt tttcttatca attatcaata taaaaatgaa acggttgatc
     4441 gattcaatcc ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg
     4501 aaaattccat cgaacattgg gtatgtaatg atatctttgt tcatggtaca tggctgtctt
     4561 ttcacgctgg agaggtagaa aaaggtaaga atcatgctgt aatcaaacac aaaggaaaac
     4621 acgtcatgaa cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc
     4681 tcatccaaga ctatgacgat gccttaaaaa gaagccctca ggagccagat aggcattaag
     4741 taggagatga acgtagagat agaaaaaatc aatgcacatg atcttatcaa aaaaaaacag
     4801 gggtacaaat taattgaaaa agctttgaga tgaaagttcc taaataaaaa agttcagaat
     4861 gataggtaaa aaaatgacta actattctag tattaggcta tttgtattat tcttctgtta
     4921 tttttatatc aaaaaatcca gaaataacta aatataggta tttctggctt atagtataat
     4981 atttacgcca cagcccccaa tgattctttt tggaatcatg atcaaggacg gacatacaca
     5041 aaaatatttt gcccattcga tccgaataga ttaacgacta atctagtaag taatatagat
     5101 tggtacgtag tcaaaccagc aaatggaaaa gttgtctatt ccaccccaag ggctttaaag
     5161 acagcatctt ccggcgtttg ataaaaaatc aaattaaagg tacccattaa ttctgggggt
     5221 acagttccaa tatcagcagc gcttatcata ggtacaagta cttttttcgc tccggcatct
     5281 tttgctactt gtaaacagtt agctaaatta tctaccttaa tcatagaacc accaattgta
     5341 atatccccta aaataactaa acttggtaat acaggtttat ttaaggaaac tgagcataaa
     5401 gctattatag ttgataacga taatgttttt gtcataccaa tgccattcat atcttgaaca
     5461 tgaacgacaa aatcttttgt cgtcaaacta atagactgac taacacttcc ataattagct
     5521 cttaaatatc ggtatgccga atccatcgct tctcttgctc cagcatctga attgatccca
     5581 gtacgttcaa actttccatt tcccgaagtc atttgcgttt ccaatttata ggtacctaat
     5641 tgcccagttt tgccataaga taccgtgtac aaatgccctg gcttccctaa cccttctgga
     5701 atcaaagttc ctccaccttg ttcaggaaca gaaacatact cttctcgctc ttcttctaaa
     5761 tcaatgtatg aaaaattgac atcataaaat tccattccac caattttctt taattgttct
     5821 tttacacgac gacgcatttc aagagcaaat cttagtattt cttcgacatc acttttttca
     5881 aatctaccat caggatacaa cagtttcatc atcccagata caatccgacg aactgcaatc
     5941 gtatcccgct gatttaattg agaaccaagt ttgaaatatt tatcaaaact atcgccaaaa
     6001 ctaacttttc gaagctcacg catgacttca gaaaaataat ctgtaatgaa gccatagtca
     6061 tttgtaaaaa aatctggtcg atatttagga atttcccaac ctggtaaata acaatgcatc
     6121 cgatccaaaa aggctgaatc cgtccccata acctccggaa atggctcaaa taaattcgaa
     6181 gttttcaata ttacatcaac gctttgatta atatttccta caaaagccat tgatgctgaa
     6241 gcattctttt cttctttgcc tctagcaaaa gatcctgaag ccatgtaatc tttcatgatt
     6301 tgaacgccat cattatcctt aaatttaatc ccagcaactt cgtcaaaagc aacacagtcc
     6361 cacataccta caagtcccat tgtacgacta gccatattat agaaaaggtt tgctactgtt
     6421 gtttgtccac ctgaaacaag gattgagttt ggtgaaattt ctttatatac atgcgattta
     6481 cctgtaccac gtggtcctag ttcacagaga ttaaaattat tttcaattaa tggaataagt
     6541 cttgctaaat gaagccattt taccctttta ttaaattgtg taggttccat accaatggac
     6601 cgtagaagta agtcaatcca ttcatttgta gtaaactcct tgcgagccat cttgtaagcc
     6661 tcaatgtcta aaccaggcat ttggattgga tcaacctttt ttgcaataaa agggctattc
     6721 caacgatcat tctcatcata ccaatattcc atcgaaacaa tacaccagat ccccccacct
     6781 aataatcgat caaatttttt cggatactct tcactaattt gaacattttt taatcccagg
     6841 ttagagaatt ctgcttcata acaatctttc ttttcattta aatgtacagt tactttatca
     6901 ataactgtat aaccacccaa ttcacgaatt tttgctttta ctttttctgc ttcatcagga
     6961 cgcacatagt ttcgagcaag aatattttta acattttcca cacctttttc aaccaatgtt
     7021 tcatccaatg acgaagcata cataccaagt aaatattcca atacataaac aggtacattc
     7081 gcaccttctt taattttctt tgttaaatct tttctgacaa tctttccggg aaagaattca
     7141 gataacttta aaatttcctg ttgttccact agaattcctc catgtgttca gtcattataa
     7201 tagcaattaa agtatatcat acttaaccag taggcttgat aatacaaaag aaaaagtatc
     7261 cacaaaaaat tgtggatacc tcgtttttat atatcaaaat caaatgattc tgcaaaagga
     7321 tta
//
LOCUS       DAZKAO010000128         7252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000128 DAZKAO010000000
VERSION     DAZKAO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.129"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..342)
                     /locus_tag="ACG8H8_002363"
     CDS             complement(49..342)
                     /locus_tag="ACG8H8_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP8987407.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(370..1035)
                     /locus_tag="ACG8H8_002364"
     CDS             complement(370..1035)
                     /locus_tag="ACG8H8_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP8987408.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1175..1807)
                     /locus_tag="ACG8H8_002365"
     CDS             complement(1175..1807)
                     /locus_tag="ACG8H8_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8987409.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1814..2881)
                     /gene="liaS"
                     /locus_tag="ACG8H8_002366"
     CDS             complement(1814..2881)
                     /gene="liaS"
                     /locus_tag="ACG8H8_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP8987410.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2878..3609)
                     /gene="liaF"
                     /locus_tag="ACG8H8_002367"
     CDS             complement(2878..3609)
                     /gene="liaF"
                     /locus_tag="ACG8H8_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP8987411.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3778..4257)
                     /gene="greA"
                     /locus_tag="ACG8H8_002368"
     CDS             complement(3778..4257)
                     /gene="greA"
                     /locus_tag="ACG8H8_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP8987412.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4393..5568)
                     /gene="mltG"
                     /locus_tag="ACG8H8_002369"
     CDS             complement(4393..5568)
                     /gene="mltG"
                     /locus_tag="ACG8H8_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP8987413.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5772..6941
                     /locus_tag="ACG8H8_002370"
     CDS             5772..6941
                     /locus_tag="ACG8H8_002370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8987414.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         2188 a         1476 c         1113 g         2475 t
ORIGIN      
        1 caaaagaaaa aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt attgtttttt
       61 aaagtgatag gttggctcct ccaggttggg atcataatca acaagtaaat catatccttt
      121 aaagaaccat tcatctgctt cctcgataaa aaaaagtgtc ccatcaattt ccactttctt
      181 tacaggatgt tctggctgtt caactgacat tccaatcgaa aaaccatcat gaacctctgt
      241 tttgccgtag acttttccaa aaaaacgtat tccgtaagtt tctggcagat caatttctga
      301 gtcaaaccac ttttgtgctt ttggcgttac cgttaatttc atcccaacaa cctactttct
      361 cttttctttc tattgattca tcttatcgtc tagaatatcc acatcgcctg tctctccaat
      421 aaccagtaga ttatcatttt cacgaacata ttcatctgct gcaggagaag cgataaccgt
      481 ttgatctact cttcgtatgg cgactactgt taagtcaaaa cgttgcctga aatttaattc
      541 agctaatgtt ttgttgaaaa atttaggatt ggagactcgg atttctgcta aagaatattt
      601 atctgacaac tctaggtaat ctaaaatatt ctttgacact aaattatgcg caatccttat
      661 tcccatgtct cgctcgggat ggacgacgcg atcagctccg atcttttcaa gtactctggc
      721 atgatactca ttttgggctt ttgctaaaat atttggtacg cccatttcct ttaccatcag
      781 agtaactaag atactggctt gaatatcttc tccaatagcg acaattacat gatcgaaatt
      841 tcttatgcct aatgatcgaa gtgtcatttc atcctgtgca tttgccacaa cagcatgagt
      901 ggctatattc atatattcat tgacccgatc ttcattacta tcaatagcca gtacttcttg
      961 accagcttcg atcagtgttc ggcagatact tccgccaaat cgtccaagac ctacaattgc
     1021 aaaattttgt ttcaccgttt tcctccttta gtatcttctt ttctcagtat aacaaagcct
     1081 ttacaaaaac aaaagcaagc atcattattg taaaaaagcc aagaaaaata atagttattt
     1141 tctcagcttt tgattatcct ctttatcatc gtttttattt caccaaacca tgtttgaatg
     1201 catagatagc ggcttgtgta cggtcttcca cttctagctt agccaatata ttggaaacat
     1261 gtgtttttac tgttttcagt gtaataaaca attcgtccgc tatttcctga ttactttttc
     1321 cttcagaaat cagcattaaa atttcatttt cccgattcgt tagttcttca tgtaatacag
     1381 gctcatttcg ccgactcatt ttttccatca tttttgttgt cacttctggt tctaatactc
     1441 gctcgccacg ttgcgttgcc cttatagcat cagcaatttc atgtgcggta gaggtcttca
     1501 acaaataccc tgctgcacct gcttcaatgg caggataaac tttttcgtca tcaataaaac
     1561 tggttacgat aatgatttta gcttctggcc aatcttttaa gattgctttt gttgattcga
     1621 taccatccat ttcttccatg actaagtcca ttaaaatgac gtccggtcga agtgtcatcg
     1681 ccttctcata gccttgtcgt ccattttcag cttctccaat tacttcaatg tcttcttgaa
     1741 tagataaata aaaagagacg cctaagcgca ccatttcatg gtcatctact aataaaactt
     1801 ttatcatcct tatctatgcc tccttcatca aagggacctt gatttctaca cttgtacctt
     1861 gacctttaaa actaatgatt tttactgttc caccaatacc agctactcgt tctttgatat
     1921 tgttcaatcc ataacttccc gttttcgttt cattcatatt aaaccctgtt ccgtcgtcaa
     1981 taatacgtaa caaaagatta ttgtctattt tgtgcaaata tacctctaat tcattggctt
     2041 tagcatgtct taatgtattt gataacaatt cttgaacgat tcggaacaag tgatcctcaa
     2101 tggaactaga tagttttaca tcttctacat cccatttcag cgaaatctgg attttatttt
     2161 gcaattcctt caataactgt tcgatacctt gctttaaact tttctcttct aaattgactg
     2221 gacgtagatg aagaagcagc gcacgcatct cagactggga agcgttgatg atctctgcta
     2281 ccattttcaa ctgcttgcga aacatttctg gtgtctcgct tttttctgct tgctccgtca
     2341 atgccgacat catcatcatt gctgcaaaca attgttgcga aactgaatca tgcagctcac
     2401 gggctaatcg atgtctttct aattctaaaa tttcttcttt ggtttgtcca tctacatatt
     2461 ggggacggct cgttacaatt tgtaattcac tagatacttc aatcattttt tcttttatct
     2521 tagtgatttc cttatcaata tctttgatgt ataaatcgtc cgatgcactt gggatcggaa
     2581 gaagcaacaa tttggattca taattgccag aagaaagtgc acgcagcttt tcttcgattt
     2641 tcccgtattt tgtcttttga ataagtgata ataatagaaa gacaattaat cctacaccta
     2701 aggaaatggc taacaaatgg aagataagcg gaacatataa taaacgtgca cgcagtaatt
     2761 ctccccacca atgactttga ttgctggcat aaaaataagt gaaaagtgaa aatagaataa
     2821 taaggaaagc cgcaatcccc gaataaacag ctagcatcgc tctggatatt tttcccatca
     2881 tattcgtatc acctcgacat ctccaatcaa ggtattagta ataatcttca atctacgagg
     2941 gttttcatca taatcactgc tgtagatttt gatttgttca tttttcaaag aaaattgctc
     3001 ttcttcaaac aagacatttc ctactaatgt cgcatgctct aatcggatgg ctacgcccaa
     3061 aggtactaag atccgtgttc tgcctatccc ttttcttaca atcacaatat tatcatcttt
     3121 tggcaaaagc gtattgccca aatctatgat agtatcacca gaaatgacgg cgatgttgat
     3181 atcgtcccat tcatagacgt cgtttccaat acgttggtta ccgaataatt gctgtctttt
     3241 ccgctcatta ttatgtgttt ttaattgctc tgtttggatc atcatgatct gtttctttcg
     3301 ccaaaaagca tttttggtta aatctatccc tgaaatctct acacctttca gaccaataaa
     3361 taaaacggca aatacagcca tcatccataa agcaggactg ttgactaagc taaaaaaaat
     3421 gaccaaactt cctaatatca attgaaaatt ttgaaattta gtacgaggat gtttgcgcat
     3481 agctagatat atattgaaaa taccgaatat cactaaaagc aaaagtccag tattgtttac
     3541 gatttgccaa acagcaaata acagcagtag tgcttcaact accacaaaaa aacgccaaga
     3601 actattcatc gctctttctc cctcctgaca cattctcatt tcatcatacc tattttttta
     3661 actgttggct ataggacttt agatgtaaga tcgtttgttg aaatctaaaa tacacaaaga
     3721 gaaaaagatc tagagtgtat catcagtaag atgaactctc tagatctttc agtcagacta
     3781 cgcttgttct actttgacga ttttaactaa catatcgcca cctggagtag caatagttac
     3841 ttcatcattt ttacgtttgc caatcaatgc ttgcgcaata ggagaatcat tggaaatttt
     3901 gccagaaaac ggatctgctt cagcgcttcc gacaatcgtg tactcttctt cttctccatc
     3961 tggtagttcg atgaatgtaa ctgttttacc aattgatacc tcatctgcat ctacaccgtc
     4021 gttatcgatg atttgagcaa aacgaatcat attttcaatt gttgtgattc ttccttcaac
     4081 aaatgcttgt tcatctttcg ctgattcata ttcagagttt tcggaaagat ctccgaaacc
     4141 gcgggcaatt ttgattcgtt caatgatttc tccgcgtttg actgttttta gttcttctaa
     4201 ttctttttct aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg
     4261 taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca
     4321 aaaaagatta ccacgctttt cttcaaatgt aaatgttttt ctaaaaatga tcaatgtcta
     4381 gagcctgttt ttctaagaat tatttacata tttttccact aattccatat gttcttcata
     4441 agttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt
     4501 ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggtccagg
     4561 tccatagccg gtattggtat acaaattata tggtgaatct acagctgtat cttcatacgt
     4621 cactaattct ttgtgttcac ctaatgcgta taaaacagag atatctgact gaagtggcat
     4681 atttgctttg attcggttaa agaagacttg tgcaatgttt ttacgatcat tttcttttac
     4741 gccttctttt tcaactagag aagctagagt taaaacttct tgtacagtca aatttttctg
     4801 cttgatagct gaataatagt tttgcatgac cgtattcgtc ttgttcacca tttcaatcac
     4861 tagatcctta agcgaagtac tcgtgtaata atcatatgtg gctgggaata agtaaccttc
     4921 caaacggtat tttgtgttct cagctttaga tgcactttcc aacagttctg ggaatttttg
     4981 gtgcaactcg ttaaagaaag tctcatcatt cactaaatct aaaaattctt tcttgtcttt
     5041 accagttacc ttcgctaccc gttcagcaat ctgatcgata tcgtaccctt ctggaatggc
     5101 aattttccca tctgcaactt ttgttggctc agaagtaccg ccttcttgca actgtttgcc
     5161 aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa
     5221 atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc
     5281 caaaatctca ccaatctgct tgttagaaga gccactcggg atctcgactt ggactaattg
     5341 gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc
     5401 aagaaatccg ccaataatca ataatgtaag agcaatcact aaaatgatct tcctcacaat
     5461 cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt
     5521 actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc
     5581 ttactatcaa gttcgtaact ttattcatta tacatgatat ttgaattggc gtgaatgatt
     5641 ttatttaaat attctatcaa aaatgtaagt agtaaatcca ttactataag attaaaataa
     5701 atgttatcaa gatacaagct attttagctt tattctttct gtaaaaaaga aaggagacta
     5761 aataaactat tatgaaaaaa ggaaaacatg ctaaaaaaag aaaatattca tttttaatga
     5821 tcgttgtcgt gttgtttagc cttagtgcgt tctctacagg aactattctt ggatggcaat
     5881 tttacaataa tgaacactct cagatagttg cccatgctca atcagctcaa aaaaagttga
     5941 tactgactac caatgaacaa cagctcgttg aaaaaatcga tcaactatta agaaaaaatg
     6001 attatattgg ctcaatttac gtaaagaaaa ataatcgcat catcatggaa aaaggctatg
     6061 gctatgcgaa taaactcacg caacaaccaa atgatccttc tctttattat cagattggtt
     6121 cggttcaaaa agcattgact actcttttga ttttaaaaca agtcgagcgt gggcaccttt
     6181 ctttagatac taagctggct caattctatc cacaaattgc tggcagccag caaattacta
     6241 tccgtgactt gctgtatatg cgctcaggct tgcaccgaac tgcttctcca accataccga
     6301 tgactgatga agaagtcgtt caatttgcga ttcagcactt aaaatttaca gattatcaga
     6361 cctctcatta tgattcattg aactttacca tattgactgg aattttgatc aaattaacaa
     6421 atcaaccgta tgaaaaatta ttgaccgatg atatcatcga acctttagga ttacagcata
     6481 caaaatttta tgaccaagtc aaaaattcat ccaatcatgc actatcctat caaatgtcta
     6541 aagaggatga ttactttaaa ccactgacag aatctgaaac agagatccat aacgaattag
     6601 ggactggtaa tatcagtatg tccgtctatg atttaaatac gctgtttaca caagttttat
     6661 ctggaaaact agtttccaaa gacttactct tctcattatg gcgtgaaaat gcgaatggac
     6721 atccatatag tggtggggta tatagcggag ataattatat cctgtcgcaa ggaaatatcg
     6781 actcttttga tacatctgct atatttagca aagattcaca ggatgctatc atcatggaaa
     6841 gcaatataaa agctgacaaa actatcaaac tgcctactgc tgatctaaga aaccaaatct
     6901 atgacctgat ggaaggaact actaagttgg caaatagctg atatgatgaa aagatagcaa
     6961 atactacttc tttgaatagc atttgcttaa aaaaagtact cgactaaacg taggaaagag
     7021 tgtgggacaa atctaaaaat caatttttgc tacagacttt aattccaaat aaacagaaca
     7081 gaaagaggtg ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca
     7141 ttatttgaca aagggcctat taaatctaaa atagtatctt tagattgcta aaatatgtgc
     7201 tagcaaatca ttcatggctt gttcttattt ttaattttat gcaataaaaa ag
//
LOCUS       DAZKAO010000129         7251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000129 DAZKAO010000000
VERSION     DAZKAO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.130"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8H8_002371"
     CDS             99..746
                     /locus_tag="ACG8H8_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP8987415.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8H8_002372"
     CDS             1139..3784
                     /locus_tag="ACG8H8_002372"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP8987416.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8H8_002373"
     CDS             4094..5407
                     /locus_tag="ACG8H8_002373"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP8987417.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8H8_002374"
     CDS             5397..6050
                     /locus_tag="ACG8H8_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8987418.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8H8_002375"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8H8_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP8987419.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8H8_002376"
     CDS             6947..7147
                     /locus_tag="ACG8H8_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8987420.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2396 a         1229 c         1593 g         2033 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt a
//
LOCUS       DAZKAO010000130         7078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000130 DAZKAO010000000
VERSION     DAZKAO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.131"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..877
                     /locus_tag="ACG8H8_002377"
     CDS             <1..877
                     /locus_tag="ACG8H8_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP8987421.1"
                     /translation="YTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELFDWAE
                     TYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPY
                     SDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQ
                     EFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANL
                     IRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQK
                     LKEQLQENTEITIQF"
     gene            941..4573
                     /gene="pglX"
                     /locus_tag="ACG8H8_002378"
     CDS             941..4573
                     /gene="pglX"
                     /locus_tag="ACG8H8_002378"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFP8987422.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4689..>7078
                     /gene="pglZ"
                     /locus_tag="ACG8H8_002379"
     CDS             4689..>7078
                     /gene="pglZ"
                     /locus_tag="ACG8H8_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFP8987423.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVF"
BASE COUNT         2667 a          957 c         1333 g         2121 t
ORIGIN      
        1 ttatacctac cctagtgctt atgaatatga gcaaataaaa aaatatcttg aagtaattgt
       61 tcaaacgtca gatgttgaag acttttttta tacaatcaaa aaatatcgcg atgaattatt
      121 cgactgggct gaaacatatg ataaaattaa aaatttccac aagcaaaata gtactcagaa
      181 aaaaatctgg gagaatgcac aagataaaat tagattaatc gaaaaaagtc ggcgtcaaac
      241 ttctagccaa gaaattgatc aattgattca tgagatgaaa accattttga aaaaggctga
      301 tccttattca gatatgatga aactaaatga gttgattcaa gaatttattg aacaatataa
      361 tcaactgtta gagttggaaa gtcaggaaat attaaataaa ttaacggaag aaaaacaagc
      421 aacatttaaa ttattagaag gaaaagattt taaggatcaa tttgtaccaa tagttgacca
      481 agaatttcaa gaactgaaag caaaaattga acaagcgaat actatagatg atcttgtttt
      541 cttatcatta aacgtagcag aacagtattt gatctttaat caacgtttcg ataatgaaga
      601 aaaccgcaga cgtcaagaag aacaagcacg taaacaaatt gaagcagcaa acttgattcg
      661 tgaaactgaa aaagatgaaa tagactctgc agcaaaagct gaagaaatag cgatagtaaa
      721 acctactatt caacagaaaa cagtaccaat gagaacactt aacgttcatg ttaaaactat
      781 taagtcagca gcagatattg atgagttaac agctgaaatt aatcaaaaac tgaaggaaca
      841 acttcaagaa aatacagaaa tcacgattca attctagtga aaagacaggc ataaaaaggt
      901 gcctgtcttt tcaaaggtta catatagaag ggaaagaaaa atggataaga aagcgataaa
      961 gaatttcgcg gtcaatgccc gtagaaaatt aattgatgaa gtaatgacta aagcaaaacg
     1021 tttaggaatt gatgaatcaa aaattgaaga tgtgaagaaa attgatagca atttacaaga
     1081 aatcgaaaaa tcaggaataa gaattcaagg aaaagaaata gcgcaaagaa acaaattgat
     1141 tgcagaacta aaacaacgtg cttctgtcac ttcttattca gaagcttttc aagagttaat
     1201 cgaagaggtt gcttatacgt ggtttaaccg attgattgcc attcggttta tggaggtaaa
     1261 tgattattta cctgacacgt atcgtgtatt atctagtgaa ataagtggga aaattgagcc
     1321 agatattatt acagatattt atgatgccga tatctttgaa gaattatcta gtaatgaaca
     1381 gcaacaagtc agaacttgga aaacagataa ttcagcagaa gcaatggaca agctgtatca
     1441 attgattttt ataaaaatct gtaatcaatt gaatgattac ttacctgagc tatttgaaga
     1501 agtagaagat tatacagaac ttttgtttac agcttcttat attaaaaaag atggtgtaat
     1561 cgctgatctt ttaagtattc cagaggatga tttctatgtt caagctggtg gacaggtaga
     1621 gattattggg tggttgtatc agtactataa tagtgagcca cataatcaag tggtgaatat
     1681 ttataaaggg attgtcaaaa aacaagatat tccggcagca actcaactat ttaccacaga
     1741 ttgggtcgtt cgttatatgg ttgacaatag tttaggaaag tattggttag atcattatcc
     1801 caatagtgaa ttaagaaata agttgaaata cttaattcca gataaatatg cttttgaaca
     1861 agcaacatct gtaagtaatt taaaaccaga agaactaaaa attatcgata acgcaatggg
     1921 gtctgggcat attttagttt acgccttcga tgtattgatg gaaatttatc ttacgttagg
     1981 atattctgag cgtgatgctg cacgtgcaat cgttgaatac aatttatatg gtcttgaaat
     2041 cgataaaaga gcgtatcaat tgtcttattt tgcaattatg atgaaagggc gtcaatacga
     2101 tcgtcttttt ttgaaaaaaa gtctatcgcc gaatttaaga gagtttattg attctcctaa
     2161 agtgacagac gagtatcaag agcgtattag tgaattgaac aatgataagt atagtgaacg
     2221 ctggaatgaa tacaaagagt taatttttga tcaatttgaa aatgggaaag aacttggatc
     2281 aatcattaag ttagtcgata aaaataatca ggcaataact aaagaatact taggaaaagt
     2341 aagagagtat atatcaaaat tcaacgaatt tacgaatatg gatattcttt atgggatgaa
     2401 ggaaatacaa gaaaaatact tgcacattct tgacgtggct gaaatgctag ttgataaata
     2461 tgtagcggta gtcactaatc cgccttatct taataaaatg gataagaaat taaaaaaata
     2521 tgtaaatgat aactattctg atgtcaaaaa agatctattt tctgtgttta ttaaattgaa
     2581 tagccaaatg ctgattgata atggatatgc ttcctttatg acgccatttg tttggatgtt
     2641 catttctagc tatgaaaagt tacgctcatt tttaattaat gaaagaaaca tttctagttt
     2701 gattcaaatg gagtattcag cttttgaaga agcaaccgtt ccaatttgta cgtttacgat
     2761 aaaaaattca aatgaacaag atggaagcta tataaaatta tcagatttta aaggtggaat
     2821 ggaagttcaa aaaattaaaa cgttagaagc aattcatcag catggtgaat gcgattattt
     2881 ttatagtact aatcaagaca acttcaaaaa aatcccagga atgccgattg cttactgggc
     2941 aagcgataac gtaatcactg attttgctca aggaaaatta actaacgatg ttgcagatgc
     3001 aaaacaaggg ttggcaaccg gaaataacaa taaatttttg cggtattggt ttgaagtaga
     3061 atttaacaaa ataaagtttg atgctaaaac attagaggac gctaaaaagt ctagaaaaaa
     3121 atggtttcct acaactaaag gtggtcgttt tagaaaatgg tatggaaaca atgaatacgt
     3181 ggttaactgg gagaatgatg gagatgaaat aaaaaattat gttaatgaaa atgggaagtt
     3241 aaaatctaga ccacaaaatc ttaattacta ttttaaaaag ggaattactt ggtcgacact
     3301 ttcatctgga caagtatctt ttagattgca gaatcaggca atttttgaaa ctaaaggatc
     3361 tgttctgttc tcaaaggata taactgactt tgaagaaata tttggctatg taaactctaa
     3421 attagttaat cactttttaa cttttatttc tcccaccttg gattaccatg aaggaccaat
     3481 gggaaaacta ccttttctta aagttacatc tgagagaaaa ttaaaaaaaa taagagaaaa
     3541 agtggatgaa aatatcgata tagctagaaa agactataat gctcttgaaa cgtcatacga
     3601 gtttagaaga aacattttgt taagttatat gtttaaaaaa gatctctcag aggcatttaa
     3661 ctgtctaagt aggcagttgg ttaatcaatt taatactcta aaatcgaatg aagaggaact
     3721 gaacgacata ttcattaatc attattctct tgaaaaagaa ttaacaaaag aggttcaaga
     3781 tgaatatatc tctgcaaggt tacctgataa ggcgagggaa attaaggatt ttatttctta
     3841 ttcaataggt tgtatcttcg gtcgttactc cctagacgaa gatggtctca tcttcgctgg
     3901 cggcgaatgg gatccttccc gctatcaaaa atttaaacct gatactgata atgttttact
     3961 catcaccgat gatgagtatt tcaaggatag ccaaattgac attgtcaatc gtttcattga
     4021 tttcgttgag attacttttg gtaaagaaac ccttgaagaa aatctccagt ttatcgctga
     4081 cactttaggt ggcaaaggga caagtcgaga aattattcgt cgttacttcg tacaagattt
     4141 ttacaaagac catttaaaaa catactccaa tcgaccaatt tattggcaat tggactcagg
     4201 aaaacaaaat ggctttaaag cgttaatgta cttgcatcgc tatacaccag atcaattagg
     4261 aaaagtacgt actgaatacc tacacgaact acaaaaagcg tatgacaatc gaatcgaatt
     4321 gcgtcagcaa caaatttcaa caagtgataa cccaaaagaa agaaatgcaa tgacaaaaga
     4381 aattgtaaag attcagaagc aattaaaaga agttcgtgag tatgacgaga agttgggtca
     4441 cttagcatta aaacgaattg aattagactt agatgatggt gtaattgtca attatgataa
     4501 gttgcagcgt gatcctaaaa ccggcgagaa attccaaatt tttgcaaaag gggtcaaaga
     4561 accaaaaaaa tagttatcta agaaagttgt tttctaacaa ctttcttttt tattcttaaa
     4621 ttgcgtgcga aattatccaa attatgtcaa tataaggggt atagaaagag aacgattagg
     4681 tgagattgat gacagatata aatacgcaac aaattgaaga aaaattaaat caacgttttc
     4741 ctaattatgg atgtaggaaa atcatttttt ggtttgattc aaatcaggat tttcttgaag
     4801 aaattgactc attggtcatt caaaatgcaa aaatctatcg attagaaaag gacgcacaat
     4861 ttaaaactaa gcgtttattg gaatatgaag atcccgaaac taactattta atctatgcgc
     4921 catttcctcg ttgggatgat aaagatgaag ataatcattt gttgagtgta ttgaattatt
     4981 cagaggaatt tagtgcagac cgcttagcga ttatcatgaa tgaattggct attccactta
     5041 gatttcatca gacagttaaa cagtatcgta atttctttaa tgcaaaggat cgcatgagaa
     5101 gttttggtga atttagtacg catatgacaa ttaataaatc agaaacgatt gaattaatga
     5161 taatggctgt tttgttgaaa tcgaaaagta ttcgattaaa tgatttactg cgtgtagttc
     5221 taaaaagctt tacagaaagt agggagaatc ctttaaggga acttgataag tatcagttaa
     5281 cagctaaatt ttgggaattt attcaaatga aatttggctt tacggatagc cagccaacat
     5341 tggaaaagct gacgatgtgt ctttatttga actattttta ttatcaaatt gatcaagaga
     5401 tgccgaagaa gttcaaagga tttgaaatac tacatttgaa agcaaacatt attactttca
     5461 tggatcagtt tatgaatgat acacgttaca tggaaagctt tgaacagcta tctaaaaaag
     5521 tatatcaggc tattgatggt gatggattat tagacagtat agaaattgat gatctaattg
     5581 aagcagacgt attttctgag attcatgcaa aaatattaat gttttatgca gaacgattaa
     5641 aggcttctga cactacaacg aaaattgcag gacaaacaat ttttgatagt ttaaagcagc
     5701 gcgaaaaaat gcatttcgca tcggattata tttatgagta tcaattattg aaacatgctt
     5761 attatcttgt ggataaaaac agattgtgct tgccgatgaa catggataat ttgctaactc
     5821 aatatgagaa ggaaatgtat tgggttgata cgcattatcg gaaatttatt tggaatagtg
     5881 atcatataga agaaaagcaa aaatatggcc aattgtatcg attagttgaa gcaaattatt
     5941 cgaagtttct tgatcaatca ggacaaattt ggaatcaaac ttttgaattt aagaatcgtc
     6001 catccattcg aaaattttat aatcaagctg taatgaaacg ctctgttaaa actgtagtta
     6061 ttatttcaga tgcttttcga tatgagttag ggatgaattt agaacgtaag ttagttagag
     6121 aaaagaagta ttcaacaaac atggctagct ttctttctgt attaccttca gttactgaat
     6181 tcggtaaagc cgccttatta ccaaacaatc aactaactta caatgaagga acagaagtac
     6241 tgattgatgg gaaaaaaacg aatggtttat ccaatcgaaa tgcgattctc cagcaacgag
     6301 atgcaaatgc tgtagcttta aattatgatg atgtggttaa gatgaataag gctgaggtac
     6361 gagaagtatt taatggcaaa tcagtcattt atatttatca cgatcaagtg gatagaacag
     6421 gtgatcactc gaatgaaaat cgagtttttg aggcctgtga tttagcgata agcgaaattt
     6481 cccgacttat taatcgcttg cataatgatg gcagtatcta tcgttttata gttacggctg
     6541 atcatggatt tatttataaa agaagccacg ttgaagaagt agataagatt gaaaatccat
     6601 ctagaaatga aaaagatcgt gtagaacgtc gttttattct tagtgaagaa gaatacgaaa
     6661 taattggtac gaaaaattac tctttgggag attctttagg aaatgatgac actagaaaca
     6721 tttgtgtccc gattacttca agtattttta agaaagctgg cgggggacaa agctacgttc
     6781 atggagggtc atctattcaa gaaatgctag tccctgttct agaaattacg gtagccaaag
     6841 gtagcaatgc aaaggaacca gtagcaattg aattaatgac tagcaaccgc agaattacag
     6901 gattatcaac cacgttagaa ttctatcaaa aaaatgcaat tagtgatgtt tatgaccccg
     6961 catctttctg gctttatttt gaagatgaat atggtgaacg tatctcaaat gaagaaatct
     7021 atgttgcgga ttcaaaaaca gaagatgcaa atcagcggtt tactaaattt gtttttga
//
LOCUS       DAZKAO010000131         7000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000131 DAZKAO010000000
VERSION     DAZKAO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.132"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..2054)
                     /gene="thrS"
                     /locus_tag="ACG8H8_002380"
     CDS             complement(114..2054)
                     /gene="thrS"
                     /locus_tag="ACG8H8_002380"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP8987424.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(2391..2570)
                     /locus_tag="ACG8H8_002381"
     CDS             complement(2391..2570)
                     /locus_tag="ACG8H8_002381"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP8987425.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(2617..4440)
                     /locus_tag="ACG8H8_002382"
     CDS             complement(2617..4440)
                     /locus_tag="ACG8H8_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8987426.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            4718..6997
                     /gene="helD"
                     /locus_tag="ACG8H8_002383"
     CDS             4718..6997
                     /gene="helD"
                     /locus_tag="ACG8H8_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP8987427.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2055 a         1496 c         1286 g         2163 t
ORIGIN      
        1 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
       61 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
      121 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
      181 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
      241 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
      301 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
      361 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
      421 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
      481 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
      541 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
      601 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
      661 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
      721 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
      781 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
      841 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
      901 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
      961 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     1021 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     1081 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     1141 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     1201 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     1261 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     1321 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     1381 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     1441 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     1501 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     1561 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     1621 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     1681 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     1741 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     1801 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     1861 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     1921 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     1981 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     2041 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     2101 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     2161 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     2221 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     2281 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     2341 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     2401 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     2461 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     2521 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     2581 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     2641 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     2701 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     2761 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     2821 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     2881 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     2941 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     3001 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     3061 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     3121 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     3181 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     3241 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     3301 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     3361 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     3421 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     3481 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     3541 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     3601 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     3661 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     3721 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     3781 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     3841 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     3901 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     3961 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     4021 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     4081 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     4141 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     4201 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     4261 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     4321 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     4381 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     4441 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     4501 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     4561 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     4621 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     4681 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     4741 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     4801 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     4861 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     4921 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     4981 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     5041 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     5101 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     5161 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     5221 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     5281 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     5341 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     5401 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     5461 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     5521 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     5581 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     5641 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     5701 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     5761 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     5821 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     5881 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     5941 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     6001 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     6061 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     6121 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     6181 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     6241 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     6301 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     6361 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     6421 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
     6481 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
     6541 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
     6601 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
     6661 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
     6721 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
     6781 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
     6841 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
     6901 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
     6961 tatggatatt tcatcttatg agaaaaaagt actgtaagta
//
LOCUS       DAZKAO010000132         6902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000132 DAZKAO010000000
VERSION     DAZKAO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.133"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..444
                     /locus_tag="ACG8H8_002384"
     CDS             <1..444
                     /locus_tag="ACG8H8_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP8987428.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="ACG8H8_002385"
     CDS             444..1643
                     /locus_tag="ACG8H8_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8987429.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="ACG8H8_002386"
     CDS             1659..2303
                     /locus_tag="ACG8H8_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987430.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="ACG8H8_002387"
     CDS             2314..3120
                     /locus_tag="ACG8H8_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987431.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="ACG8H8_002388"
     CDS             3142..3372
                     /locus_tag="ACG8H8_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987432.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="ACG8H8_002389"
     CDS             3886..4194
                     /locus_tag="ACG8H8_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987433.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="ACG8H8_002390"
     CDS             4196..4618
                     /locus_tag="ACG8H8_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987434.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG8H8_002391"
     CDS             4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG8H8_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP8987435.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="ACG8H8_002392"
     CDS             6239..6574
                     /locus_tag="ACG8H8_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987436.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6902
                     /locus_tag="ACG8H8_002393"
     CDS             6715..>6902
                     /locus_tag="ACG8H8_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987437.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2647 a         1036 c         1420 g         1799 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgag
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     6901 tt
//
LOCUS       DAZKAO010000133         6886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000133 DAZKAO010000000
VERSION     DAZKAO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.134"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8H8_002394"
     CDS             complement(<1..182)
                     /locus_tag="ACG8H8_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8987438.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8H8_002395"
     CDS             517..951
                     /locus_tag="ACG8H8_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8987439.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8H8_002396"
     CDS             974..1294
                     /locus_tag="ACG8H8_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987440.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8H8_002397"
     CDS             1435..1626
                     /locus_tag="ACG8H8_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987441.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8H8_002398"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8H8_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP8987442.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8H8_002399"
     CDS             2112..2375
                     /locus_tag="ACG8H8_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987443.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8H8_002400"
     CDS             2399..2587
                     /locus_tag="ACG8H8_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987444.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8H8_002401"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8H8_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987445.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8H8_002402"
     CDS             3070..3441
                     /locus_tag="ACG8H8_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8987446.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8H8_002403"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8H8_002403"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8H8_002404"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8H8_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987447.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYTDQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8H8_002405"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8H8_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP8987448.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8H8_002406"
     CDS             5250..5447
                     /locus_tag="ACG8H8_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987449.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8H8_002407"
     CDS             5553..6035
                     /locus_tag="ACG8H8_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987450.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8H8_002408"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8H8_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP8987451.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1057 c         1353 g         2145 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgt
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DAZKAO010000134         6750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000134 DAZKAO010000000
VERSION     DAZKAO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.135"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACG8H8_002409"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACG8H8_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249876.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            602..817
                     /locus_tag="ACG8H8_002410"
                     /pseudo
     CDS             602..817
                     /locus_tag="ACG8H8_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            955..1926
                     /locus_tag="ACG8H8_002411"
     CDS             955..1926
                     /locus_tag="ACG8H8_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8987452.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2065..3378
                     /locus_tag="ACG8H8_002412"
     CDS             2065..3378
                     /locus_tag="ACG8H8_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFP8987453.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3421..4287
                     /locus_tag="ACG8H8_002413"
     CDS             3421..4287
                     /locus_tag="ACG8H8_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8987454.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4284..5132
                     /locus_tag="ACG8H8_002414"
     CDS             4284..5132
                     /locus_tag="ACG8H8_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8987455.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5146..6618
                     /locus_tag="ACG8H8_002415"
     CDS             5146..6618
                     /locus_tag="ACG8H8_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP8987456.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6635..>6750
                     /locus_tag="ACG8H8_002416"
     CDS             6635..>6750
                     /locus_tag="ACG8H8_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8987457.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2121 a         1133 c         1443 g         2053 t
ORIGIN      
        1 taaattttaa gtctacttat aatttacttt ggaggttttg aatgaatgaa attaagcgca
       61 ataaaaattc ttttggtgtt tatttaggag agcctagctc ggaacagtta aataaaggtg
      121 aagctcgtca tgctctatgt cgaaatatat tttatggtag aaaaggcaaa ctttatcaaa
      181 cttattacga cggtatggag gaacagctca acgcattagg attagtaact aatgctatca
      241 tatattggaa ttcactatat ttagaaaaag tctttgaaca aattaaaacg gaagggttcg
      301 actgttccga agaagtgtta cgcaagcaat ctccattaat tagtgatcac attaattttg
      361 ttggcaaata tagtttccgc tatgaaaaag aattatcgaa tggaatacta cgtccgttaa
      421 atatagagaa ttattgagaa tgttcgtctt ttggttccta ccgtgcattt tcgcacggat
      481 gttccagata ctatttttga atgtataaaa ttgaaaacac aagaatacta ataaatcaac
      541 gattcttgtg tttttaattt ttcactaccg tgcattttcg tttctatgtt ccatgtaccc
      601 caatcttgaa aaattaatta aagacttaga taaagccatt gacgacttag tcgtggtgct
      661 accggagtat caatgtttaa ccagtatccc aggtattggt cctgtttagg ctgctggaat
      721 actcgctgaa attggtccaa ttgaacgttt ttctgatgaa ataaaattga caaagtatgc
      781 cggcctatat tggcgacaga gtcaatccgg taaatctgaa tacgaaaatt tttttacgaa
      841 aagcttcacg tctaatatct attttaaaat aaaaatattc ctgctaattt ggaatcgttt
      901 ccttcttttg gtataataat acctgaaatt cttttgagag gagttgttcg tgaagtgaaa
      961 actactatga aggatgttgc tgcgttagca ggagttggtg tgggtacggt atctagagta
     1021 ataaatggag taaaggtgaa agaggttacc aaagaaaaag taatggctgc aattagtgag
     1081 cttaattatg agcctgatga atatgccaga ggtatgaaaa cgaatagtag caatacaata
     1141 gctcttattt tacctacgat ttggcatccc ttcttttccg agtttgccta ctatgtagag
     1201 gaagctctca gccaaaaaaa ttataagctt tttttgtgta atgcgaacgg cagtgcggaa
     1261 aaagaaaaag aatatatcca aatggtcaag cagagtaaag tagacggaat tataggtatt
     1321 acctattcag acattgataa atatgtatca tcaaatttac catttgtcag tatagaccgc
     1381 catttttcgg aacaggtttc ttatgtaacg gcagataatt atttaggtgg gcaaattgcc
     1441 gctcaagaac tacttactcg taactgtaaa aacttgtgct atgttggggg tgtttcacct
     1501 taccctaacg aaacaaacaa tcgtaaagct ggtttttaca atatttgtga ggaaaataaa
     1561 atagctgtga aggtattgga tatgcctgaa ccaattagca atttagagga acaattgctc
     1621 gctttttttt cggataatcc tgaaattgat agcattttta ccatcaatga tttcatggct
     1681 ttggatgtga taaaagccca aaaaaaacta gggaaggaag cacctaagga ttttcaaatt
     1741 attggttttg atggagtcaa gctatcgttg caaaaagact atttactatc aacgattgta
     1801 cagccgatag aggaaatggc caaggtttca gtagaaacac tcatggcgat gattttaaat
     1861 gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc acgctggaga gacgactaaa
     1921 gagtaaaaaa ccggtttata acgatttttt tgagataggt tgacagaaag tgatttcaat
     1981 gatatcttaa aaccaattaa ttggaaacgt taccaattaa ttgaaaaata aaacggaaac
     2041 gtttcaaaaa taggaggaac atcgatgaaa aagagaatgt tagcaattgc agcaatttta
     2101 gcattaacag ctggaggact tgcaggatgt ggcagtaagg agaaaagttc aggtagcagt
     2161 gattcaggag aaattaagat ttgggttcag ttttctgatg aaacgcctga aggaaaagct
     2221 tgggcagaga ttgtgaacaa ttttaatgat gaatacgagg gtgaatataa agctgttact
     2281 gagtatattc ctcgtagcgg aagcggcggt ggatatgagg ataaagtaaa tgctgcaatt
     2341 acaaccaata cccttcctga tgttattacg ttagatggac cgaatactgc agcttatgct
     2401 cattcaggag tcattgctcc attagatgat tatttgaaag atgcggacat ggatgatgta
     2461 ttagacagca ttattcagca aggaacctac gatggcaaaa tgtatgcgtt tggtttctca
     2521 gagtccagtg tgggtgtcta ttacaataag tcgttgttca aagatgctgg aattacagaa
     2581 gatcaattgc cgacgttaga agatccttgg acttggacgg aatttcaagc aatttgccaa
     2641 aagctgactg ataaatacaa aacgcctgca attgatatgc aaatggcgtc gggagacgaa
     2701 atgctgacct acgcatatac accatttgta tggtcgaatg gcggcgatgt cgttaatgct
     2761 gatggaacca aagccgaaga ctattttaac ggtaaaaaat ccgttgaagc gttggaattt
     2821 attcaagaat tggttgaaaa aggttacacc acaatcagtc ctattgaaaa aggatttgaa
     2881 actggcaaat atgccatgct gctgagcggt tcttggacaa tggcagattt agaggaaaac
     2941 tatcaagaga ttgattatgg tattttgcct taccccgttt cagatgcaac tggggagtta
     3001 gtttcaccaa ccggaagctg gcagttagca atgactgaga aaacagagaa aaaggatgct
     3061 tctgcggcct ttattaagtt tgcaaccagt acagaatcta gtcgtattat gagtttaggg
     3121 aacagtgttc tacctatcag aaattcaaca gttgatttaa ttaaagatga agtgtcggaa
     3181 ggaatgcagg tattaattaa gcaaaaccaa atttctggac atgcccgccc agtggttgta
     3241 gcttatccgc aagtatctag agcattccaa caagcagttc aagatattag ctacttcgag
     3301 gaaaacccag atgtacaaaa agtagcagat agtcgcgctg ctgaaatgca acaggcaatt
     3361 gatacttcac taaaatagaa gtttctaaag ggggccaatc aagtgttaaa aaaaccgaca
     3421 atgaaagaca atttgatagg ttacggcttt ctagcgccag ctttgctgct gctgacgatt
     3481 tttctgatca tacccgtaat catggtaatt tattatgcct ttactgatta ttatttgtta
     3541 acgccagatg ctagacaatt tatcggctta gaaaatttta agcgactgtt cgctgatcca
     3601 atttttgtaa aaagtatttg gaacacggct aaatttgttg tttggattat tccggtccaa
     3661 ttaggggcag ctttaggaat ggcacttatt atcaacaaaa atcgtaaagg aaatatcttt
     3721 tttaaagtag ccttttttgc gccagtcgtg atgtccttag tcgttatttc gattttatgg
     3781 ttgtacttgt taaatcctaa tgaaggtctg gttaatacgc tatttgcaaa gattggaatt
     3841 gcttcgcagc cttttttgac tagtcccaaa caagcaatgt atacgcttgt tttcgtatcg
     3901 gcatggcaag gtgcaggata tcagatgctg ctgtttttag ggggaatgca aaatattccg
     3961 caagacgttt atgaggcagc cgaaattgat ggtttttcta agtttcagca atttcgctat
     4021 atcaccatgc cattattaaa gcccacggcg atttttattt tacttaccac attgatttcg
     4081 gcctttaaac taatcattca gccaatggta atgactcaag gtggaccaat gaattctact
     4141 atgacaatgg tttattacat ttaccaaact ggatttacag accgtatggt aggctactcc
     4201 agctccattg cgttaatctt taccactatg attggtttaa ttacaattgc tcaaagaaaa
     4261 ttggtaaagg aggatgatcc agcatgacca agaaatttgg accgctaatg atcgtagaat
     4321 attgtttgct gttcctttta gcggcattat tcattttccc aatgctgtgg atggtagttt
     4381 cctctatgaa gcctgaagca gatgtctatc aaaatttagc ttccttcaaa gcttttttac
     4441 ctagtttgaa tccagctaat tggtttaaaa cttatcaaga agttatttcc cggttcagtg
     4501 tgggaactta tttattaaac agtatttttt atggtttaac ctttgcctgt gggtcaatca
     4561 ttgtaaactc tttggccggt tttgcctttg cgaaggttaa tttttcaggc aagaaaatta
     4621 tctttggagc tttgttagct ctgctgattg tcccgatgga gacggtttta attccgcaat
     4681 ttaccatcgt taattcacta ggcttagtga atactcgctt ggcagtgatt ttaccagcga
     4741 tggccagcgc gtttaatatc tatttgttta gaaatttctt tatcgcgatt ccagaagaaa
     4801 tcattgaatc agcgaaatta gatggtgcaa atattgttaa aattttcttt cacatcatgc
     4861 tgccaatgtc taaaccagcc atagccacag taggtgtctt atcctttatc tccagctgga
     4921 atgattatat ttggccgtta atggtcttaa ctgacagcag caaattttca atgcaagtgg
     4981 caattactac gatcaatact acacagccag tttatatcaa tcaagtgatg gctgttttaa
     5041 ccattgcaac gattccactg attattgttt atatcgttgc acaaaaatat attctgcagg
     5101 gacttggcgg ttctggaacg ggtataaaat aggagtgaat agtgaatgtt gcaaaaactt
     5161 ttaaaggcca atgagtttat tgagaaaaat gctcaaacag tcaatcaaga ataccggaat
     5221 aaatttcatt tgatggctcc gattggctgg atgaatgatc caaatggttt tgtttattat
     5281 caagaggagt atcacttgtt ttatcaatat tatccttacg acagcatctg gggaccaatg
     5341 cattggggcc atgctaaaag caaggatcta attcactggg agcatttgcc agtagcactg
     5401 gcaccaggtg aaagctatga caaggaaggc tgtttttccg gcagtgccat agaaaaggat
     5461 ggcaagctgt atttgattta tacaggacac aatgttgtgg aagggcaagt tcgacaagtt
     5521 cagtgtttgg cagtttcaga agatggcatt cactttgaaa aatatgccgg aaatccaatc
     5581 atcgcagaag atcacttagc ttctgttgca acgaccgaag atttccgcga tcccaaagtg
     5641 tttcagcgaa aagaagacta ttttactgta gtcgctacta aaactgcaga tgctcgtgga
     5701 caaattgtga tgttccattc caaagattta ctaacttggg agttttattc cgtattgctg
     5761 gagggaactc cagaacaagg cattgtatgg gaatgcccag acttgtttga actagatggc
     5821 aaagaagtct tgattctttc tcctattcaa atgactccac aaggcaatgc ttaccaaaat
     5881 acgaactcaa ctgttgcttt catcggtact gtcgattggc aaacaggcaa atttctagta
     5941 gagaattttc atgaaatcga tggcggttta gatttttatg caccccagac ctgtgaaaac
     6001 gaagcaggcc aaagaataat gattgcttgg atgcaaatgt ggcagcgtaa aatacctacg
     6061 aatgatctgg gccatggttg gtcaggttcc atgaccttgc ctagagagct gagggtggct
     6121 gataatcaat tgttgcaacg gccagtttca tcaatttatc aagtaattga tccgcaggaa
     6181 aggattgaat gggcgaagac tgaagagaat cgcttcttac tgaaggactt agtatatgac
     6241 cagcactatt tacgcttgga aatcaattta aaacaggcaa cgaaactaga acttcactat
     6301 gcgaagggac ccgaccatac attactgctt aactatgatg ttgaacggga gtgctttact
     6361 gcttccaggg aacacatgcc gtatgattta tcaggagctg aaaaggaagt gttgaaaaag
     6421 cgcagtgtga cgattccttt ggaaaataat tgtctaacca tcgaaatttt ccgtgatact
     6481 tcagctattg agctttttct aaatgatcgt ctgactatga gtacaacttt ttatgagaca
     6541 gaaaaaggac aggattttct tttgctggca gacagtgaag taaccttttc attggaaagc
     6601 ggcttaatta atctatagaa gggagagctc agtgatgatt tatggttcaa tcgaagcagg
     6661 cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag
     6721 ctttccgaca acgacacctg aggaaacaat
//
LOCUS       DAZKAO010000135         6693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000135 DAZKAO010000000
VERSION     DAZKAO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.136"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..477)
                     /locus_tag="ACG8H8_002417"
     CDS             complement(19..477)
                     /locus_tag="ACG8H8_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP8987458.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ACG8H8_002418"
     CDS             complement(490..1821)
                     /locus_tag="ACG8H8_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP8987459.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ACG8H8_002419"
     CDS             complement(1832..3274)
                     /locus_tag="ACG8H8_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8987460.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ACG8H8_002420"
     CDS             complement(3261..4025)
                     /locus_tag="ACG8H8_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP8987461.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="ACG8H8_002421"
     CDS             complement(4129..5004)
                     /locus_tag="ACG8H8_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8987462.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5259..5343)
                     /locus_tag="ACG8H8_002422"
     tRNA            complement(5259..5343)
                     /locus_tag="ACG8H8_002422"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Leu,seq:caa)
     gene            complement(5361..5432)
                     /locus_tag="ACG8H8_002423"
     tRNA            complement(5361..5432)
                     /locus_tag="ACG8H8_002423"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5398..5400),aa:Gln,seq:ttg)
     gene            complement(5442..5514)
                     /locus_tag="ACG8H8_002424"
     tRNA            complement(5442..5514)
                     /locus_tag="ACG8H8_002424"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:His,seq:gtg)
     gene            complement(5576..5660)
                     /locus_tag="ACG8H8_002425"
     tRNA            complement(5576..5660)
                     /locus_tag="ACG8H8_002425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Leu,seq:caa)
     gene            complement(5681..5751)
                     /locus_tag="ACG8H8_002426"
     tRNA            complement(5681..5751)
                     /locus_tag="ACG8H8_002426"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Cys,seq:gca)
     gene            complement(5797..5868)
                     /locus_tag="ACG8H8_002427"
     tRNA            complement(5797..5868)
                     /locus_tag="ACG8H8_002427"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Gln,seq:ttg)
     gene            complement(5880..5952)
                     /locus_tag="ACG8H8_002428"
     tRNA            complement(5880..5952)
                     /locus_tag="ACG8H8_002428"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5917..5919),aa:His,seq:gtg)
     gene            complement(5955..6028)
                     /locus_tag="ACG8H8_002429"
     tRNA            complement(5955..6028)
                     /locus_tag="ACG8H8_002429"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5994..5996),aa:Trp,seq:cca)
     gene            complement(6035..6115)
                     /locus_tag="ACG8H8_002430"
     tRNA            complement(6035..6115)
                     /locus_tag="ACG8H8_002430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6079..6081),aa:Tyr,seq:gta)
     gene            complement(6117..6192)
                     /locus_tag="ACG8H8_002431"
     tRNA            complement(6117..6192)
                     /locus_tag="ACG8H8_002431"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6157..6159),aa:Phe,seq:gaa)
     gene            complement(6228..6300)
                     /locus_tag="ACG8H8_002432"
     tRNA            complement(6228..6300)
                     /locus_tag="ACG8H8_002432"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6265..6267),aa:Asp,seq:gtc)
     gene            complement(6310..6382)
                     /locus_tag="ACG8H8_002433"
     tRNA            complement(6310..6382)
                     /locus_tag="ACG8H8_002433"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6347..6349),aa:Val,seq:tac)
     gene            complement(6398..6469)
                     /locus_tag="ACG8H8_002434"
     tRNA            complement(6398..6469)
                     /locus_tag="ACG8H8_002434"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6434..6436),aa:Glu,seq:ttc)
     gene            complement(6484..6573)
                     /locus_tag="ACG8H8_002435"
     tRNA            complement(6484..6573)
                     /locus_tag="ACG8H8_002435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6537..6539),aa:Ser,seq:gga)
     gene            complement(6613..6686)
                     /locus_tag="ACG8H8_002436"
     tRNA            complement(6613..6686)
                     /locus_tag="ACG8H8_002436"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6650..6652),aa:Asn,seq:gtt)
BASE COUNT         2007 a         1483 c         1099 g         2104 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaact ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5161 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5221 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5281 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5341 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5401 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5461 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5521 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5581 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5641 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5701 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5761 ttatcaatct ataaaataaa gaataaatta tagaaactgg ggtagctgga ttcgaaccaa
     5821 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg
     5881 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
     5941 cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac
     6001 cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg
     6061 aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt
     6121 ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg
     6181 agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg
     6241 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
     6301 agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
     6361 ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc
     6421 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg
     6481 ctacggagag taagggattc gaacccttga gacagtttgg caccatctac atgatttcca
     6541 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
     6601 tcaaaatgag aactccggca gtaggactcg aacctacgac atcatgatta acagtcatgc
     6661 gctactacca actgagctat gccggaataa cat
//
LOCUS       DAZKAO010000136         6622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000136 DAZKAO010000000
VERSION     DAZKAO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.137"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACG8H8_002437"
     CDS             complement(111..635)
                     /locus_tag="ACG8H8_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP8987463.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG8H8_002438"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG8H8_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP8987464.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG8H8_002439"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG8H8_002439"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP8987465.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACG8H8_002440"
     CDS             complement(2357..3037)
                     /locus_tag="ACG8H8_002440"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP8987466.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACG8H8_002441"
     CDS             3163..4032
                     /locus_tag="ACG8H8_002441"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8987467.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACG8H8_002442"
     CDS             4058..4573
                     /locus_tag="ACG8H8_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP8987468.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACG8H8_002443"
     CDS             complement(4620..5183)
                     /locus_tag="ACG8H8_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987469.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACG8H8_002444"
     CDS             complement(5238..5417)
                     /locus_tag="ACG8H8_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987470.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACG8H8_002445"
     CDS             complement(5494..6003)
                     /locus_tag="ACG8H8_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987471.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACG8H8_002446"
     CDS             complement(6099..6479)
                     /locus_tag="ACG8H8_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8987472.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1862 a         1398 c         1173 g         2189 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga ca
//
LOCUS       DAZKAO010000137         6539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000137 DAZKAO010000000
VERSION     DAZKAO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.138"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..514
                     /locus_tag="ACG8H8_002447"
     CDS             152..514
                     /locus_tag="ACG8H8_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFP8987473.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            531..1142
                     /locus_tag="ACG8H8_002448"
     CDS             531..1142
                     /locus_tag="ACG8H8_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFP8987474.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(1244..2152)
                     /locus_tag="ACG8H8_002449"
     CDS             complement(1244..2152)
                     /locus_tag="ACG8H8_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8987475.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(2194..2508)
                     /locus_tag="ACG8H8_002450"
     CDS             complement(2194..2508)
                     /locus_tag="ACG8H8_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP8987476.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            2807..4738
                     /locus_tag="ACG8H8_002451"
     CDS             2807..4738
                     /locus_tag="ACG8H8_002451"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8987477.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            5031..5582
                     /locus_tag="ACG8H8_002452"
     CDS             5031..5582
                     /locus_tag="ACG8H8_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987478.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            5895..6437
                     /locus_tag="ACG8H8_002453"
     CDS             5895..6437
                     /locus_tag="ACG8H8_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFP8987479.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         2153 a         1087 c         1264 g         2035 t
ORIGIN      
        1 taaaaaattt aataataaaa tttaaattcc tattaagttg taacacatga aattgcttat
       61 aatagtgtat tgaaattaag gtagattgac aaacgagtga tcaaatagat atactacctt
      121 caaacgaccg tttgaatgtg aggtgagatg aatggaaaaa gtttgccaag tgactatcgt
      181 acataaagaa aaagtaaatg taataaaaaa aaaattggat caaaaagatt tttcaaactt
      241 acttgtgtta gggaaatgtt tcagcgattc ttctaggatt aaaattttct atgctttaga
      301 aacctataag gaaatgtgtg tctgtgattt agcagaaata cttactgcta gtgtcgctac
      361 aacgtcacac catttacgtt ttttaaaaaa acatggaatg gcaaaatcac gtcaagatgg
      421 aaaagtagtt tattattctt tagcgaatga agacatcctt tccgctattc gtgttttttt
      481 aaagttatca gaaaatctat ctatgaaatc ttagtttgga ggaagttatt ttgttacaaa
      541 gtattttttc agctatcgct gtttatattt ctaccagtat tgattatttg tttatcttgc
      601 tgattatctt ttctcaaagc cacacaaaaa aaggtattcg tcacattttt tggggtcaat
      661 atcttggaac aggtattttg gtagccgtaa gtttgtttgc tgcttatgtg ctaaatttta
      721 ttccgcaaga ttggatcatt ggtcttctcg gtctaattcc gattttctta ggaatcagag
      781 tcgcttttgt tggcgaagag gaggaagaag aagaggaagt cgttgaaaaa ctcgaatcta
      841 gaggaactaa tcgcttattc tggacagttg ccttaataac gattgcttct ggaggcgata
      901 atttaggtat ttatattcca tactttacct cgttagcttt ttcagaaatc attattgctt
      961 taatcgtatt cgctatctct gttgcggttc tctgctatat cagttacaaa ttagccaaga
     1021 tttcttttgt ttccgaaacg ttagaaaaat acgaacgaat cattgtccct ataatgttta
     1081 ttgcgttagg aatttttatt atgatagaga acgggacgat acaaacgcta ctaggttttt
     1141 aaaaatcata atcacgaaag ataaaaagag aggcagcgac taatcaaaat cgctgccttt
     1201 tttgcttttc aaataatgaa gtatttttta gcgtagaact atcttacgtg ttgtgggagt
     1261 atcccttttc aagctcttct ttgcttgtag ttatttctat tgtgatatga ttctctacgc
     1321 ttgatttgtg tgcgacttct gcaatttctt cttttatttc cctagccttg tctatgtctg
     1381 aagggtctat aagaaccgtg atgataaggg cgttttcttc cccgtccagt gaccaaatgt
     1441 gaaaatgtga gaggtctttt acagcaggaa catttttaat ggagtccgcc aagctcttta
     1501 cgtttacatt ttcaggcgaa ccattcaata aaattttcat tgtggtgaaa aattcaggaa
     1561 cggttttgta gaggatataa agtgcaatca aaatcgataa gataggatcc agacgatatg
     1621 cctcggtaaa gttcattaga atactcacaa tcaagactcc aatccaccct aatacgtctt
     1681 ccagcatatg gaggttcaac atgctctcgt ttttggatgt gcctttgctt aaaatccacg
     1741 cagcatatcc attcaatgca atagctacga gagaaagcca gaacatgcca ctataattta
     1801 cgggttgtgg gtcaatcaaa cgtggcacac tacgatagat cataaaaaac gagccactaa
     1861 ttagcacaac gccagttatc agcgcgccca gtaaagaaaa gcggttatag ccaaaagtga
     1921 accgttcatc ttgttttttc tctgaataac cttgaaaaaa ccaagcgaat ccaattgaaa
     1981 tagagtcccc taaatcatga actgcgtcgg acataatgga cacactattg aaaagaaagc
     2041 caaagaaaaa ttcgcttata gagaaaataa gatttatgaa gaaagcaatt ttaatattct
     2101 ttttcggttg attctgattc gagtgattat ggtctttatc tgattttgac atttaaaaac
     2161 ctcctaagtt tgtatgctac atactaattg gttttatttt tctaattcat taacatgagt
     2221 taagacttgt tcgagaatac taaaaacatg aaggtcatct aaactgtaaa gcatactttt
     2281 tccttctctt cttgatttca ctaagcgtgc gtctttcaac gtttttaatt gatgagaaat
     2341 agcggactgt tccatgtcca gagaaatagc gatatttcca acactacact cttttttctg
     2401 taaaagaaag agaaccgaca atcgtgtagg atcactaatc accttaaata gtttgcttat
     2461 tgacagaata gatcctttat cgattggaga agttgttttt atatccatag tgtttttatt
     2521 cctttcatat gaccttccgt tcatatctat attaccatat gagcgaaaca atgctaatct
     2581 tttgtttata aaagagaagt ggcgttctct ttatcttgac aaaatgtata tagcgatata
     2641 aaatgagtat acaaacatat gattaattag tcatatgtaa ggtggattca ttacgattta
     2701 cttttggaat aaacgaaagg agtcagaaat agcagaaatt tatgtgattc aagaagtaaa
     2761 tgatggaaaa caaaggaatt ataaattata tagggaggca atagaagtgg aaactatcca
     2821 agaacaacag tctcaagaaa agcataatca taatcacaac cacgatcacg accatgatca
     2881 tgggaaaatg ccagtcgttt tgtactttgt tggtttagca ttagcagtga ttgctctatt
     2941 tttgaatgaa gattatcagt tggtacaaaa tgttttgttc tcaatcgcta caatcagtgc
     3001 cgggtatcac gttattcttc ttgaaggcct tggagaaaca attgagaatt caaaagcaca
     3061 aaaaaagttc atacctaatt ctcatatttt aatgggatta gcggcaattg gagcttcttt
     3121 gatggggaat ttttgggaag gaacattatt gattcttatt ttttctggcg cacatttttt
     3181 ggaagattat gcagaaggaa gaagcaaacg agaaataacg aaattactcg aaatgaatcc
     3241 aacgactgcc cggttaatcc tgcctgatgg tagtacaaaa aatgtggatg tcagtgaatt
     3301 aaaagtggga gatcaacttc aagtgttgaa tggcgatcaa gtacctattg atggcgtcat
     3361 tttgtctgga tctacatcca ttgatgagtc gtctattact ggagaaagta tcccgaaaga
     3421 gaaatccaaa ggagatggcg tcttcgggag tacaatcaat ggaacaggta ccttcacgat
     3481 gaaagtcacc aaagaaaaca aagatacagt attttcaaaa attttacaat tagtgaatca
     3541 gaaccaagat aaccaaacaa aagctgcgag tattatccaa aaatttgaac cgaaatatgt
     3601 cacagttgtt ttaattgcca ttcctctatt catgttattg gctccttttc tacttgattg
     3661 gacatggtca caaagtgtct atagaggatt ggtcctttta gttgcagctt caccgtgtgc
     3721 tttagcagca gctactgtct cggcaacgct atcgacgact tctaatttag ccaaaaaagg
     3781 agtcctttca aaaggtagtt cttacctgtc tcaattagcc gatattaaag caattgcatt
     3841 tgataagacg ggcaccttga ccaaaggaaa acctgaagta accaattatt attttgctga
     3901 ttccgtgaac gaagaaaaca tgattgatat cgtagtagct cttgaaaaag aatccaatca
     3961 ccccttagca gatgctattc taagaaagtt tgagcaaaaa aacaaactaa ctattgaagt
     4021 agaaaaccaa attggtaaag gattgacggg agattacaaa gggagaaatt atcgtatagg
     4081 aaaaccgact tcgtttgatg atgtttcaga tgaatatagt cgtttaaata atgaatgggc
     4141 gtcagaaggt aagacagtcg tatacgtagc agtagatgaa gaggttatag gccttattgc
     4201 ccttatggac gttccgagtg aatatgctaa agaaaccatt gaatatttta ggaaacaagg
     4261 tatacacacc acattaatta ctggtgactc agaaatgacg ggaaaagcgg ttgctaaaca
     4321 attaggaata gatgaagtga ttgcgaatgt catgccggaa gacaaatcca gaattataga
     4381 cgaacaaaaa gaaaaatacg gcgtgaccgc catggttgga gacggtgtaa atgatgctcc
     4441 tgctcttgtt aatgcagatg tgggaatcgc tatgggagat gggactgatg tggcagtaga
     4501 ggtatctgat ttagttttaa tgcaaaacaa tttatctaaa ttggtacaat ctcataatat
     4561 ttcttcgaaa atgaaccgtg ttatttggca gaatattatt ttttcaatgg ccgttgttgc
     4621 ttttttaatt gtcgttagtt tcttaggctt aacggatatt gcaatcagtg tcattattca
     4681 tgaaggaagt actttggttg ttattttgaa tggacttcga ttgctaaagg cagtctaaag
     4741 aatgattgca caacttcaaa ttaatcgagt aatagagata attaaggatt acactacatt
     4801 ttttatctct attacttgtt tatatttatc tagatgttca aagtcaaaat gatatatcaa
     4861 ccatactctt gtggcgcggg ttgaatataa gatacatgaa attggataaa atcatgtatc
     4921 aagaatccta tccttatagg gttctttttt tttactcccc ctaaacacac gatttcgtgt
     4981 tgtaataact ataatcatgt attcgaacag actaaaaagg agggattgtc atgggctata
     5041 atgttcagcc actacggtcg caacaggaga ttaatgattt tttattttgc ctgcgacgga
     5101 ataaaaatgc ggaacgtgac gttttcttgt ttctaattgg aattaacagc ggtctgcgca
     5161 tgtccgacat cgttaaactc aagaaaaaag acgtgattac ctcaaaaaac ccgcgcatcg
     5221 tggagcaaaa gacagggaaa acacgcattt tatacttaag cagcttacag gacttgattc
     5281 acggctatac cgcaccctta gatccggagg cttacttgtt tcccagtact aagggcggcc
     5341 atttagaagt caatacggtc taccagatgt ttcaaaaggt cgcgaagctg ttaggaaggg
     5401 acgatatcgg cacccacacc ctacgaaaaa cctttggtta ccactattac aagaaaacca
     5461 aagacgtggc caccttaatg gaaatctttg gtcacagcag cgagaagatt accaaacgct
     5521 acatcggaat taatgaagat gaaatcagtg aaacgttatt aaattttcgg ttaggttttt
     5581 gattgtatta tataatagat aagcacagta agacggttat aatcacccac agtattggac
     5641 aaatcaacct ttttttggta tattatatat acaaatgaga gcaggctcat agtcaacttg
     5701 atataaatcc ggaacataag ggtccggcgg gtggcgagca ccaaaactaa actcgataca
     5761 aatccggaac gtaagggtcc ggcgggtggc aagcaccaaa actaaacctg tacaaaaggc
     5821 tgctcttttg agcagctttt tttattggga aaaaggagca ttcttcgcac gcggcaaaaa
     5881 aagaatacaa acgcatgtct ataaaagaga caacccaaat taccagacag ttaaacgcga
     5941 tttataaggc tgctcatctc ttacaagaac attttgtaga taaaaaagta tcctttgtcg
     6001 gagaagtctc aacggtagcg attatttttt cgacaactaa ttttatgcac ctctgtggca
     6061 ttgattatcg cagaggcaca cacttgtttt ttcaagatgc cttagacagg aaaataaatt
     6121 tacaggatat tcaaatcaaa acggatggga cgacctttca gaaattacaa gtaataggta
     6181 gccttgattt attattgggg aagcatattt ctattgttgg cagaggtgtt tactcttctc
     6241 ttcgctatga tgcagctatt cgaacaagga aaaaaatatt ggctctgtct ttgaaacaaa
     6301 acggattaat ctatatccct atctctttgt taaacctatc atcaaaggaa attggacctg
     6361 gacaaaaagt tactggcatt tttagcgagg atttaacatc cggggaactt aaaatgatta
     6421 tggaagtcat cgattaatca ttaatagtga tatgcgacgt cattttgaag taattattct
     6481 attttgggtt ataaaataaa gcattttaag acgatttcaa atcattcatg tgggttatt
//
LOCUS       DAZKAO010000138         6387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000138 DAZKAO010000000
VERSION     DAZKAO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.139"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG8H8_002454"
     CDS             330..1175
                     /locus_tag="ACG8H8_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8987480.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG8H8_002455"
     CDS             1456..2676
                     /locus_tag="ACG8H8_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP8987481.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG8H8_002456"
     CDS             2673..5393
                     /locus_tag="ACG8H8_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8987482.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG8H8_002457"
     CDS             5377..6321
                     /locus_tag="ACG8H8_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8987483.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAZKAO010000139         6202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000139 DAZKAO010000000
VERSION     DAZKAO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.140"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8H8_002458"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8H8_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8H8_002459"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8H8_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP8987484.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8H8_002460"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8H8_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8H8_002461"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8H8_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP8987485.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8H8_002462"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8H8_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8987486.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8H8_002463"
     CDS             3582..4271
                     /locus_tag="ACG8H8_002463"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP8987487.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8H8_002464"
     CDS             4285..5787
                     /locus_tag="ACG8H8_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8987488.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..>6202
                     /locus_tag="ACG8H8_002465"
     CDS             5800..>6202
                     /locus_tag="ACG8H8_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP8987489.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEK"
BASE COUNT         2001 a          982 c         1131 g         2088 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aa
//
LOCUS       DAZKAO010000140         6045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000140 DAZKAO010000000
VERSION     DAZKAO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.141"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..523)
                     /gene="yajC"
                     /locus_tag="ACG8H8_002466"
     CDS             complement(143..523)
                     /gene="yajC"
                     /locus_tag="ACG8H8_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP8987490.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(620..1765)
                     /gene="tgt"
                     /locus_tag="ACG8H8_002467"
     CDS             complement(620..1765)
                     /gene="tgt"
                     /locus_tag="ACG8H8_002467"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP8987491.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1983..2681
                     /locus_tag="ACG8H8_002468"
     CDS             1983..2681
                     /locus_tag="ACG8H8_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP8987492.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2849..5488)
                     /locus_tag="ACG8H8_002469"
     CDS             complement(2849..5488)
                     /locus_tag="ACG8H8_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8987493.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1700 a         1323 c         1107 g         1915 t
ORIGIN      
        1 gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat
       61 gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc
      121 gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt
      181 ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag
      241 tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt
      301 actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg
      361 aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta
      421 acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg
      481 ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa
      541 ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta
      601 atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt
      661 caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt
      721 tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt
      781 catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat
      841 cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac
      901 ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa
      961 acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca
     1021 agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt
     1081 ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga
     1141 ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa
     1201 ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg
     1261 tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa
     1321 aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata
     1381 aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc
     1441 gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc
     1501 agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc
     1561 ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg
     1621 acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc
     1681 cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac
     1741 gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca
     1801 actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact
     1861 ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta
     1921 tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa
     1981 tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg
     2041 gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact attcttctta
     2101 ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag
     2161 aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca
     2221 ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa
     2281 aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg
     2341 gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag
     2401 ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt tactacgata
     2461 tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat
     2521 tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata
     2581 tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa
     2641 caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga
     2701 ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca
     2761 ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta
     2821 aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat
     2881 ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc
     2941 agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa
     3001 cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta
     3061 aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat
     3121 aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc
     3181 tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc
     3241 ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa
     3301 tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa
     3361 gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc
     3421 ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt
     3481 tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg
     3541 tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg
     3601 tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat
     3661 ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt
     3721 cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt
     3781 ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta
     3841 attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga
     3901 tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca
     3961 ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg
     4021 gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc
     4081 gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat
     4141 cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca
     4201 tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg
     4261 cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt
     4321 tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt
     4381 tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt
     4441 tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc
     4501 agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt
     4561 tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt
     4621 ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc
     4681 cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc
     4741 gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca
     4801 aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc
     4861 cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact
     4921 catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg
     4981 ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat
     5041 caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca
     5101 ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg
     5161 ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga
     5221 tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa
     5281 tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt
     5341 tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt
     5401 catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt
     5461 cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa
     5521 aaggtactta ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga
     5581 ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat
     5641 gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata
     5701 gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga
     5761 gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa
     5821 gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa
     5881 gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat
     5941 tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc
     6001 ttccgaggta caagaaagcc gagcttatcg agagctcggc ttttt
//
LOCUS       DAZKAO010000141         5825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000141 DAZKAO010000000
VERSION     DAZKAO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.142"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..620
                     /locus_tag="ACG8H8_002470"
     CDS             183..620
                     /locus_tag="ACG8H8_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987494.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            715..1599
                     /locus_tag="ACG8H8_002471"
     CDS             715..1599
                     /locus_tag="ACG8H8_002471"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8987495.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1821..4394
                     /locus_tag="ACG8H8_002472"
     CDS             1821..4394
                     /locus_tag="ACG8H8_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP8987496.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     K"
     gene            complement(4467..>5825)
                     /locus_tag="ACG8H8_002473"
     CDS             complement(4467..>5825)
                     /locus_tag="ACG8H8_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP8987497.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT         1838 a         1039 c         1231 g         1717 t
ORIGIN      
        1 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattatta
       61 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc caccctagat
      121 atttttctgt ataatcttaa ataaagaaaa cgatattaaa aaatgcacgt agaagtagat
      181 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
      241 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
      301 atcaattttc cgttttggta ttaggcacac taattatagc ggggattgtt gcatttatta
      361 ttcaaggtcg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
      421 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
      481 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
      541 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatgggtat
      601 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
      661 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
      721 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
      781 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
      841 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
      901 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
      961 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     1021 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     1081 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     1141 gaagcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     1201 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     1261 ggaaagaagg aagaaattga gagcagtatc ttgctgatag gactaactaa ctcagtaggc
     1321 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     1381 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     1441 gatgaatcta cgaaaaatct agcctatctt acttgtgagc aattagttgt gtcattagct
     1501 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     1561 atcaaagtgt taccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgaaga
     1621 gttgtcagga ggacatcggt tgttcggaat aagggactgt gacaagactt ttgtcacagc
     1681 ctttttttgt acaaaaaaga aattttcacc taacagaaaa atttgccata aaaatacata
     1741 ttttaaatat gaaagtacaa ttacaaataa attaaaacgt taaaataaaa ttgtcaaaac
     1801 gtcagagagg atggtggaga atgaatcaat atgtaaaaaa gctgattgcg tcgattttgt
     1861 ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa acaagcgaat
     1921 caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa tcaattactt
     1981 ctgaaactac agaatcccaa agtgaggcgc ctacttctaa tacaaggcaa gaagaaaata
     2041 agacaagtga gacctcagaa gtagctgaac aaacgtctga tagcagtgaa gaggtacagg
     2101 ttagagttgt ttcagcggat gggtacagcg aagctgccac aacagaagaa ctgacgcagc
     2161 ttctcgcaga cgaatcagta gctaagattc gtttaactca gcctttgact ttagacaggg
     2221 aattggagat caagcgagat atcgttatcg attttggcgg acttgctcat aatttcggca
     2281 cacatcacat ctatatcaat gagacacctc agtcgatcga gtttcagaat tttaaaggaa
     2341 cggcagctca tccaggtggg ttgataccaa atatcgatgg aaatgccatt atcattgctt
     2401 atatgtccga tttttgggga aacagatatc attttacggg agaaataaaa tttaccggga
     2461 cacttgactt atatgatggt tctaaattag gcctgattta cgcccctcgt gcaactgtca
     2521 cattagatgg tgtttccggt gtattggatg tccaaccagt gaaagaaggg atgaatgccg
     2581 ctccgggtaa agcttatttt tgtagaagct atcagctaaa tgtaatcaat ggtagtcagc
     2641 tttatggacc atatctagga aaattctatg gttttctaga ttcaggtgat gagacgggca
     2701 gtggaaaaac agcaccagga ctccatatct tgagtggttc aaaggtctct cttgactatg
     2761 atcgggcaga tggcgtcagc gatgaaggag aggctgtcga tacgctaccc gggaatgtca
     2821 catttaaagt cagtggctca ggaagtgaat tttcggtaaa tacaaagatc aatattacca
     2881 acgacaacaa tcgcggaatt atccaaatga gaggctcagg atcaagagtc gctgtgtcga
     2941 atgatgggaa aatcacaatc aatacagcga ctactggcgg atttcgcctg caaggagatt
     3001 ctagtcaaat ccatgtatca agtggtggac aaatctcggt caaaatggct ggagatggcg
     3061 accaacctgg aaacaatggg atgcgttttg tagggaaagg actttctttg atcgtagaag
     3121 gagccggatc agtcatcgat attgataagc aaagtggtcg ttctagcgca gttcgttttg
     3181 agaacggtac gcaaaaacta acagtaacta atggaggctc attgaaggta agaaatgcag
     3241 gggatggcaa atcttatgtg aacagactca atcaaggaaa ccaagcgatc caattttatg
     3301 acgcttctgg ttttctagaa agtccagggt cagctgattt tacggttact ggagaaaaat
     3361 caaatatcga tatacgcgca gattccggag ctaccgtaga tacaactgga gatattgatt
     3421 tgaactttat ggctggcgaa aaaacttatt tagtcatggt cgggaaaact ggggaagata
     3481 agactggcat catcagtggg gatgtgttga atgtagactt agaatctcct acctatttcg
     3541 atttccaaaa caaacggatc ggaagcgaga ctaatggcat aggtggctgg gtatttcaag
     3601 gaaatggcga ttcttctttg aagatcgctc attcagaaat tgctttatgg cgaaaggcag
     3661 gagtaacgtt cgataacttt gaaaaagatc cggattattt ttctgatatg acagacttat
     3721 cgatcaatgg aaccgactta ggtaattttg tatcaagccc tagtgcaggt ttaacaaatg
     3781 aatttgctca gacaggtgcc ggaatgaaaa attataatcg aatcagtgcc aataaccagc
     3841 ttccgatgat tgaatcctta agagtaccaa cgaatgcaga taaaaaaatc tatgggcatg
     3901 tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc ttggacaggc gaagtagaag
     3961 tgactttacg tattacttat gcagatacga cgaaatcacc tgtagaaatt actgctctca
     4021 cacaaggaca aacagatgaa aatgaaggga caggatataa tccgtgggga gaaggcgaac
     4081 aaggcggttt gtttgaggtt caagtaccca acgaagaaag actaacaacg ggggatcaag
     4141 tgcaaatagt tgctgctaaa aaagtaagag gcggccaaga agtgacagaa ctggactctg
     4201 ccaaaacgac agtcgatgtg attcctcctg agcctgctaa attaagtacc acattgatca
     4261 atgcagcttc tcgctcaatc acaggtacag gcgaagcagg agctttggtt tcattgaaaa
     4321 aaaatcaagc tgttttgtcc gaaacaacta ttgatgatac aggtaaattt gttctttcta
     4381 tcccagaaaa gtaagcgccc catagaacag tacctgtacg tcaaataacc cgacctatag
     4441 aatagaatcg ggaatgtttg gcgtttttag ttacgagaaa aatgttgttg tacatccttg
     4501 ttccaaggca acagttgatc taaagtttct gctgaaagta cgggaagatt tggtatctta
     4561 tcaaatagaa actctagata ctttacagga ttcaatccgt taagttctgc tgttttatat
     4621 acactcagaa tgattcctga agttcgtgct ccttcaagac ttgtggaatg caaccaattt
     4681 ttccgtccta tcaccagctc tttgatattt cgctccgcac gattatttgt gagttctaaa
     4741 cgaccatctt caaaaatagg ttcaaaacca cttcgtagtt tacacgcata ttgaacggct
     4801 gcatccaact tggattttgc aacaggatct atcgttgcca tccactcata aaaagcaata
     4861 aataatggac gcagttcttg ttgccgacag cttaatcgct tctcaggaga aaagtctttc
     4921 catttctttt ctaggacaaa ccatttatcc aattgtttga ctgcttgagc tgcgggaata
     4981 tccgtgtttt tctttccttt tggaattgct tcgaaaaatt tccgccgaac atgcgcgaaa
     5041 caatagacaa gtcgtacact ttccacggca ttatacccgc tatagccgtc acagtgtaaa
     5101 taccctgtaa actccttgag aaaagttttc ggaacatctc cagcacgact ttcgtcgtgg
     5161 tgataataaa cgatgggatg ctgactgtct tttcctgtac aaaactgcca gtaataggta
     5221 tctgttttgg cactttcaat cacacgataa cttgtttcat ccccgtgtag aatctcttct
     5281 tgattaagat agactcttaa tcgttctcct aaagggtcta acgcgttttg cactgcttta
     5341 atatgccaat tggtgatatt atctctcgaa atatccaacc catgtgtttg tttccaatag
     5401 ttctcttgcc gataggcagg aactttctga ccaaatttca tccggatcgt ttccgccact
     5461 aaactattgg aacctaagct attagaaatc aaaggtttag gaacgatcgc ttttttaaaa
     5521 gaagaaagtc cttctttttc gcagctttta caacaatagg tatgttgata atgggtgaga
     5581 caatccaaca tagcctgatg gaaacgaatt tcttctcgta cttttgtccg tccgatatct
     5641 ttcatctgtg aaccgcaatg ttcacaaaat tgttcttcca aaggaaggag atgttctttc
     5701 tctgttatag gtaagtgttt gattttgtct gctttcgttc ctacaactcg ttgatgtcct
     5761 tttactggaa taatggtggc agctgcttct ggaacactta cagaaatttc ttcatcacta
     5821 aaaag
//
LOCUS       DAZKAO010000142         5771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000142 DAZKAO010000000
VERSION     DAZKAO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.143"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..1167
                     /locus_tag="ACG8H8_002474"
     CDS             13..1167
                     /locus_tag="ACG8H8_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP8987498.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1140..1583
                     /locus_tag="ACG8H8_002475"
     CDS             1140..1583
                     /locus_tag="ACG8H8_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987499.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2717..3685
                     /locus_tag="ACG8H8_002476"
     CDS             2717..3685
                     /locus_tag="ACG8H8_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8987500.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3695..4183
                     /locus_tag="ACG8H8_002477"
     CDS             3695..4183
                     /locus_tag="ACG8H8_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP8987501.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4479..4760
                     /locus_tag="ACG8H8_002478"
     CDS             4479..4760
                     /locus_tag="ACG8H8_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987502.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4762..5178
                     /locus_tag="ACG8H8_002479"
     CDS             4762..5178
                     /locus_tag="ACG8H8_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987503.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2148 a          981 c          945 g         1697 t
ORIGIN      
        1 taacacaaag gcgtgattat tttgaaaccc aaaaagaaaa tttatctagg tctcctcttt
       61 tccttgatga ttggaatttt tagtatagga attacaaata cctatgcttc agaaaattct
      121 aatttcaata tcaactccca accaattgaa gaacaagcaa ttaattttgc gaaaactgat
      181 tttgaacact ccataacttc aattctttta atggaaaata acaccgaaac tgatattaac
      241 tatactcttg gttcgccatt taaaatgctc gatggagaaa atacggcaaa tagttctatt
      301 ttctttccag tattttcaga cgctgataat acgattaaat atgtttatac cgtcaaaaaa
      361 aatacaaatg gagaatattc aggaagtatt tcacaatttt tagcaaaaga acttcaaaat
      421 ctagtagata gtaatataac agatacaacc ttattttttc aagataatag catttattat
      481 tccgataatg gacaaattaa actactttgg aaatcacctt taagtacaga aaaaaatggt
      541 gtttcttccc ttacagaaaa aaatgatctt atatccgtaa atataaaaaa taaaattaaa
      601 aatttttcta aaaaacaaaa ttttcctgcg gttaaacgtg taaaaagtgt ctcctccgga
      661 gataacttta ttatgattaa ttggcgtata gcagaaaccc aaacaagttt tccgtggtgc
      721 gctgcttacg tagaagctgc tatcttatgt aacaaaacag atgtacgtgc tacttcagct
      781 agagccatta tgcattatac ctatcctaat ttaaacaaca gtcaaattga atcaaaaagt
      841 cttaatcaag atcaaataat aaaatatgca aattactgtg gtgtttatcc taaaaaaata
      901 gcacgaatac taaacctttc agaggtccaa acacaactaa gaaaaggaaa tgccatttat
      961 atgggaactg ctggttatgg aaattatagt aacagccgtc atgcacttgc attatttggg
     1021 tgggtcaatc aaggtggtta tcaaacttac tatgtgtgga atccttggta taactaccca
     1081 actgtaataa gtgcaaatgc aggaccagtt acaattcctg tacctggagg tggttttact
     1141 tgggacaata ccattacaaa ttggtaaata aactcattgc tttagttatg ggtctagtgt
     1201 tagttctttt cttaactagt tgttcaaaaa aacaagaaat acaaaagact gatctgattc
     1261 cttttctagg aacttggcaa actaaagatc attcagaacg tctagtaatc actaatttaa
     1321 atacttcaaa aaatatagaa caatatacca tcttagtaaa agagaacaat cataaaattc
     1381 aaatcattga taaaaattta aatataaatg aacaagtttt catttacaaa gcaacaaata
     1441 atttactaac actgcgctcg aataaaaaac aattagatta cacttttagt ttggattctc
     1501 caaaaaagct cgtatttatt ttgacaacga atgatgggac agaagggaca gctaaaccaa
     1561 ttgaattttt taaagctgat tgatgaatag ttgtttgata ttacaaaaca ataatttcac
     1621 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagc cttttttgta
     1681 ttgtggagcg tgggttttga gggggattca agggggagct tgcgcccctt tgacaagcgg
     1741 ttatttgcac caaatacgta ggtatttggc tagcaaatat agagcttgcc aagcccttta
     1801 gtgtgttttg tagaaaggga ccttatagtc actttcacat tacaaacgat acgtcctatg
     1861 gacgtatcaa aagggtggga cttatgagca aattaaagca aaatttggct agcaatcctt
     1921 ctaaaaaata cacctattga tcagagtcaa aataaatcag ttttcgagtg agcgaatcca
     1981 aatttgcaac tctgaagctt tgcaaatgag tgtgccggct tccgtaaaag tctaggtaac
     2041 attagtcgat atatacatag ggctctaatt tgcgttttaa ggcgttttaa acgttctggt
     2101 atgtaattat accaaagcga ctttaaaacg ccttaaaacg aattttagag gttctgtaga
     2161 acgttttagg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa aaacaagata
     2221 agggatttta tctagacttg tcttaaaacg ccgtagatga gcgctcagag cttctaaaag
     2281 gactccttac gaactgtttt taagcaaagg agcctttagg cgactgcgca gtttaatgtg
     2341 agagcgtagc gatcacacct ttaaagcgac cgtagggagc agattcccag tttaggaaat
     2401 ttcgcgaacc tgtttggaga aaaaaagtag gaaaatttta ggcgggaaat tttgcccccc
     2461 tcttttataa tctcttttat aacctctttt taaacctctt taccgtcggc tgtatcctta
     2521 ctctcccaag gtgtgcagaa tcttttaggg gtctttttgt ctgtttttag gggtcttttt
     2581 gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     2641 ctttaattag ggggttgacc cccattagaa agctctttat aattttgtta agtagttaat
     2701 cttgaaggag gattttatgg caaatgaaat tgtgaaatac caccatgaat taaatacgat
     2761 tcctttacgc aaatttacgt cagtagaaat gaatttattc ttctctataa tatctagaat
     2821 gcgtgatgta ggcgataaaa aagtacaatt tacattcgaa caattaaaag atttaagcaa
     2881 ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt atgacaaatt
     2941 aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac gatttgtttt
     3001 atttaatcaa tttaaaatcg atggaaaagc tgatattcct tttgctgaaa tacaagtcca
     3061 tgagaaagcc ttacccttac ttaataatct tgaagaatgg gttcgatatt ctctccaaca
     3121 attcaatgaa ttagaaagca gttattccaa aaccatgttt agattattaa aacaatacag
     3181 aaccaaagga attgccaatt tttcaaaaga agactttcat gaattacttg atatccctaa
     3241 aagttataga gaaagtgata taaataaaaa aattataaag cctattcgcc aagaactaac
     3301 cgcaatcttc aaaggtttga caatccaaaa gagatacgga aaaggacggg gaaagcctgt
     3361 cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact ttaacaaaca
     3421 tgctccaacc aaagaagaac aaaataaagc tttggaacta gctaatcaac aggttcaatc
     3481 tgaaaaagaa cctacttcat tacaagaaaa aatcaaagaa caaaaacgaa aattagaact
     3541 tttacaacag ttagcagaat tagaaagaaa acaaaatacc ccaagtaaga aaattaacaa
     3601 acaaatagca gataacaatt ccgatattac cccagaattt aaagaaaact tactagataa
     3661 tctccaaaac ttatttaaag aataaggggt ttttatgaca gataaaacta tcaaagaatt
     3721 agcagatatt ctaggtgttt caaaagataa agtgaagtat caactgagga aattacctag
     3781 taattttacc tacaaaaaag gaaatattac ctacctgaaa aaagaagcag taacaacaat
     3841 tacaaaggtt ttatcaggta atgaatcggg taacttacct agtgaattac ccgattcatt
     3901 acctaccgat tacctactca aggagattga aactaagaat aatcatattg aagaaaaaga
     3961 taggcaaatt gaaaaacttc aaatagcttt agaaacgcag caaaaattac tcgaccagca
     4021 acaacaactc accctacaag ccaatcaaca aatcaaaaaa cttcacgaac aactccagct
     4081 aacctatgca gaagaatctt cagaaaaaga acccctttct gttactgaag aaactcaaaa
     4141 aggacaaaac aaggagaaaa aatggtggca attctggcaa taacccaaca atttttattc
     4201 ataaaaattg ttgggttact ttcttttttt gcttttacaa gcatagcgac atgatctcac
     4261 gtcgctattt tttaaagagc ataaggaaac gcggtatcac tttcttacga aaggtgctag
     4321 gtaggctgtg cctaccacgt ttctaagaaa aaaatttgct atcacttttt cgtcattgtg
     4381 gatagcttat gtatagtgtt tcggcaaaaa acagctatac aaatactata tttgtatgat
     4441 atacataaat tatacacaaa tgcgaaagga attcttctat ggacatttta gttcggaata
     4501 ttgatccaaa agttatttca gaaatagata gacgttgcca agaactcaca acaaaaactg
     4561 gcaaaaaatg gagccgtaat gcgtacctaa aagttctaat cgaaaacgat tttgaccagg
     4621 cacttaggca ttacaaaaaa gatcaattcg atttattact cgaaaaattt atcgatatac
     4681 aagcaaaaaa tactgaaaca ttgaacgact atgttcatac aaacaaccaa ctcattgaaa
     4741 cacttatcgg aggaatataa catgagaaaa acttctgttc aaaaacacgt taccctttat
     4801 cccagtcaca ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     4861 tcggaagcca ttcgttttct atgtatgagt tatgaaccta atactaatac gacaactacc
     4921 agcaaccaag caaaactaaa tgcgatggga aaagaaattt ctattatgtc cgaaatcatt
     4981 gcagaatttg ctgatttata tctaaaagat actggtgttc ttatcggtac aaattctaaa
     5041 gtatatcaag aagtgaaaga aaaaattgaa aatcaactca atccctcccg acgaaatcaa
     5101 cccaaaaatc gtcaaaaaga aaccaatcaa acattcactt ctagaaattt tgacccaaca
     5161 aactttctaa aaagatgaaa aagaaaccga aaattttttc tatttctcag tttctttttt
     5221 agggggcagt cttccagcaa gatttgcaag ttaccggtaa cttgcacaaa aaaagaaaaa
     5281 tccgcttgtg cgaatccttc tttttgcatt ttggggcgct gccccaatct ccaaatatcg
     5341 gttatttaat agcgtggtaa ctcaaaaaaa tagaacgtac tctgaaataa aaaaagactg
     5401 ctctaacgag cagccaataa aaaataatga gcgtttattc ttgaaaaagc agcgcgctta
     5461 tgaagcgctg ccaaaaattt accatatttt atgaaaaata acaagtttct aaaaaggaat
     5521 cggttaagat tcacaaagaa tcgtaaacag acaatagcgg aaaatcaagc ttccgaacgt
     5581 accttttagc tagaccaatg aaaaatcaca aagtgagcgt ctctatccat tggtagaggg
     5641 gctacttggc atagtgtaca ttatgtctac tagctcgata aataacgatt atgttcccaa
     5701 aacaatattt tatgaactat aacaaaaatt tgttatcgca tattgtatat tttaattata
     5761 ggtgtatatc a
//
LOCUS       DAZKAO010000143         5634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000143 DAZKAO010000000
VERSION     DAZKAO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.144"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..730
                     /locus_tag="ACG8H8_002480"
     CDS             158..730
                     /locus_tag="ACG8H8_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP8987504.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="ACG8H8_002481"
     CDS             875..1579
                     /locus_tag="ACG8H8_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8987505.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="ACG8H8_002482"
     CDS             1807..2145
                     /locus_tag="ACG8H8_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987506.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8H8_002483"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8H8_002483"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP8987507.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="ACG8H8_002484"
     CDS             3131..3364
                     /locus_tag="ACG8H8_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP8987508.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="ACG8H8_002485"
     CDS             3562..5466
                     /locus_tag="ACG8H8_002485"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8987509.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
BASE COUNT         1805 a          947 c         1291 g         1591 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa attt
//
LOCUS       DAZKAO010000144         5595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000144 DAZKAO010000000
VERSION     DAZKAO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.145"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..863
                     /locus_tag="ACG8H8_002486"
     CDS             6..863
                     /locus_tag="ACG8H8_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8987510.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQRRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ACG8H8_002487"
     CDS             885..1394
                     /locus_tag="ACG8H8_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP8987511.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ACG8H8_002488"
     CDS             1431..1832
                     /locus_tag="ACG8H8_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987512.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ACG8H8_002489"
     CDS             1944..2297
                     /locus_tag="ACG8H8_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987513.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ACG8H8_002490"
     CDS             2331..2774
                     /locus_tag="ACG8H8_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8987514.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8H8_002491"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8H8_002491"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8987515.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ACG8H8_002492"
     CDS             3869..4582
                     /locus_tag="ACG8H8_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8987516.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ACG8H8_002493"
     CDS             complement(4789..4938)
                     /locus_tag="ACG8H8_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987517.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="ACG8H8_002494"
     CDS             5334..5534
                     /locus_tag="ACG8H8_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8987518.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1940 a          856 c         1217 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcga agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaatt
//
LOCUS       DAZKAO010000145         5575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000145 DAZKAO010000000
VERSION     DAZKAO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.146"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..766
                     /locus_tag="ACG8H8_002495"
     CDS             65..766
                     /locus_tag="ACG8H8_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP8987519.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="ACG8H8_002496"
     CDS             763..1884
                     /locus_tag="ACG8H8_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP8987520.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="ACG8H8_002497"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="ACG8H8_002497"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP8987521.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="ACG8H8_002498"
     CDS             3263..4171
                     /locus_tag="ACG8H8_002498"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8987522.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="ACG8H8_002499"
     CDS             4206..4478
                     /locus_tag="ACG8H8_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP8987523.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="ACG8H8_002500"
     CDS             4489..5535
                     /locus_tag="ACG8H8_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP8987524.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1811 a          970 c         1211 g         1583 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaa
//
LOCUS       DAZKAO010000146         5525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000146 DAZKAO010000000
VERSION     DAZKAO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.147"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1920
                     /locus_tag="ACG8H8_002501"
     CDS             55..1920
                     /locus_tag="ACG8H8_002501"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8987525.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            1934..3377
                     /locus_tag="ACG8H8_002502"
                     /pseudo
     CDS             1934..3377
                     /locus_tag="ACG8H8_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            3485..4186
                     /locus_tag="ACG8H8_002503"
     CDS             3485..4186
                     /locus_tag="ACG8H8_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8987526.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            4312..4824
                     /locus_tag="ACG8H8_002504"
                     /pseudo
     CDS             4312..4824
                     /locus_tag="ACG8H8_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(5222..5431)
                     /locus_tag="ACG8H8_002505"
     CDS             complement(5222..5431)
                     /locus_tag="ACG8H8_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP8987527.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         1756 a          936 c         1243 g         1590 t
ORIGIN      
        1 ttttttcaat ctgggttttt attttttaaa taaaaaagaa agaaggaaaa aacaatggat
       61 aatcaagcag ttggtcgtcg tgtatgggaa gcggtaggag gacaaaagaa tgtcaaaagt
      121 ttagtacatt gtgctacgcg gttgcgattc agattaaaag atgaatcttt agcagatacg
      181 caaaagttaa aagaagaccc ggacgtgatc caagtcgtac aaagtggcgg tcagtatcaa
      241 gtagtgattg gcagtaatgt agcagatgtt tatcaatcga tcgtggatga ggaaggtctt
      301 gcagtttcgg aggatggaaa agaagagtcc aaaaatattt tgaatcgtct gatcgatatt
      361 atctctagta tatttacacc ttttctaggg gcgatggcag ccgccggggt attgaaagga
      421 ttcttatcat tggcaacggt gatgggctgg ctgacagctg atagcggtac ttatcaaata
      481 ttgtttgctg cagcagatgg tgtctttacc ttcttacctg tcatgcttgc ttttacagca
      541 gctaaaaagt tcaaagccaa ccagttttta gcagtagcaa ttgctatggc acttgtgtat
      601 ccagctatca cagcagttgc tggtgcggga gaagctatca gtttctttgg gatcccagtg
      661 attctttcac catctggtta tacttcttct gtcataccaa tcattctagc cgtatgggtt
      721 caaagtaaat tagagccatt cgtaaaaaaa gtgattccgc aatttctgca aatgatttta
      781 gttccgcttg tcgttttagt agtgatggtt ccattaacat ttcttgcttt aggaccaatc
      841 gggacggttg ccggtaatgc tttaggcggt ctcttcaact cgatttatgg atttagtcca
      901 atcgtagcag gtctaatcat gggaagttta tggcaagtat tcgtgatgtt cggcatgcat
      961 tggggattcg taccgatcat gtttttgaat atcgaacaat atgggtttga tgtattgatg
     1021 ccaatgcttc ttccagccat cttggcacaa ggtggggctg ctttagctgt agcattgcga
     1081 acaaaagata cgaaacttcg agcattaggg atttcttcta cagtgacttc gttatttgga
     1141 atcactgaac caactgtgta tggtgtgaca ttacctttga agaaaccatt cattgccgct
     1201 tgtatttctg gaggtattgg tggagctatt atcggatttt ccggagtaaa agctttctcc
     1261 agcagtttgg tcagcttatt gacgattccg actttcatta atacggtgga tggtgtagag
     1321 tcaaatgtga cagtagcagt aatagctaca ggaattgctt ttgttcttgc attcgtaggt
     1381 acattgattt taggatttga cgaacaaaca caagacaatc aattagaaaa caaacatgcg
     1441 aatgcaggag aaccgattac ttcagcacgg cataccttaa aaagtccttt aacgggtaaa
     1501 gtactgccat tgagtgaagt accagaccaa gttttttctt ctggcgtgat gggaaaaggt
     1561 atcgctattg atccagaagt aggagaacta gttgcacctg cagacggaga gatcacgact
     1621 atttttccaa ctggacatgc tgtagggatc acaacgactg acggagcgga gattttgatt
     1681 catatcggga tggatacagt agaattaaat gggaatggct ttgagatctt agtcaaacaa
     1741 ggggatctcg tcaaagcagg agatttattg attcgttttg acatcgaagc aattagagca
     1801 gctggctata gcgtaatcac tccagttgtc attacgaata ctgatgcatt tgccgatata
     1861 cttgaattag accaaaaaga gattattgca aacgaagatg ttctggcaat tgtaaaataa
     1921 ggaggaaatc aggatgcaat cacgtttttc agaaaaattt ttatggggtg gagcaactgc
     1981 tgccaaatca attagaaggg gcttatgatc gagacggaaa aggactatcc gttgcagatg
     2041 ctatgcctgg aggtaagcag cgctttgcga ttatcggcag tgaagaattt gactggacga
     2101 ttgatcaaga aaaatatatt tacccgaatc atcgaggaat cgatcattac gatcgtttca
     2161 aagaagatct tgccttgttt gcggaaatgg gattcaaatg ttatcgtttt tcgattgcct
     2221 ggacacgaat ttttccaaat ggagacgaag gtactcctaa cgaagcggga ttagaatttt
     2281 atgatcagct gattgatgag tgcttaaaat atgatattga acctgtgatc acgatttctc
     2341 attacgaaat gcctcttcat ttggcaaaag aatacggtgg ctggaaaaat cgaaaactga
     2401 tcgacttcta tgaacgattt gctcagacag tattagagag atacagcagt aaagtcaaat
     2461 attggatgac tttcaatgaa atcaattcgg cctttcactt tccagcttta agtcaaggat
     2521 tagtaaaaag caatggagca ggagagtacc aaaatatttt ccaagcatgg cataatcaat
     2581 tcgttgcaag tagcaaagcc gtaaaaatcg gccacgaatt gcgttcggat atccaaatcg
     2641 gctgcatgat tatctatgca acaacttaca gcatcgatgc caatccagtc aatcaggcag
     2701 caacaatgat ccaaaaccaa gaattcaatt ttttctgcac ggatgtccaa gtacgtggag
     2761 aataccctgc atacacagcg cgtacccata aaaaatatgg agtcgatccg gaaaaattag
     2821 agcaaacgga aaaagacttc cagttgctaa aagaatatcc agtggactac atcggattta
     2881 gttattatat gtctacggca atcaatgaga ctgatccaca agcagcaact tcagaaggga
     2941 acttgctggg aggagtaaaa aatccgtttc ttgaggcaag tgaatggggc tggcagatcg
     3001 atccagaagg gctgagaatc gcattgaatg aattatacaa tcgttaccag aaaccattat
     3061 tcattgttga aaacggtctt ggagccattg atcatatgga agaggatggc acgattattg
     3121 atgattatcg gatcgattat ttacgtcgtc atatcgaagc gatggctaat gcagtagcag
     3181 acggtgtaga tttgatgggc tatacgcctt ggggatgtat cgatcttgtc agtgcatcaa
     3241 caggagaaat gagtaaacgt tacggcttta tctatgtgga tctagacgat gaaggaaatg
     3301 ggacactgaa tcgatcaaag aaaaaatctt ttgattggta taaagaagta attgcctcaa
     3361 atggcgtaaa cttgtaatta aagcagctga atgaaaaaga acacacttct cacatgtgat
     3421 ttgtgttctt tttttatttt ttatgtgcta ttcttttagt aatagtaaag caaagggtga
     3481 cgttatgaaa aggacacgtg tactatactt ggaagtcgca gataaaatta aagaagatat
     3541 tttctcagga aaatatccag tcggttcgat gttgccgaca gaaagtgaat tagaagaact
     3601 gtttcaagtc agcaaaatca ctgtccgaaa ggcaatcgaa ctgctggcat cagatgaata
     3661 tgtcgcaaag aaaagtggtc gtgggacgac ggttctaagc aatcgtcctt acaatcgatt
     3721 atccaaagcg gcaagcttta cgcaaatctt ggagaactcc cgatatgaag tccggaaaga
     3781 aacgctggat ttgcaattgg tccatataaa gaaggaagat cctttgtttt cctttttcgg
     3841 tgagcaagct gtttgtttta agcgcttata ttatttgaac aatcagcctt atatttattt
     3901 tgttcattat cttccacaag aaatgatgca attcagcaga gaagaatttg gcaaagaatc
     3961 tttgtatcgt ttgctgacga aaaaaggata tactattgac tcattttctg atgatttctc
     4021 ggcagtattt ttaacagaag aagaacaagg aattctgaaa acagacgcac agatcggcat
     4081 caaacggata cggaaatcgt ctacgcaaat tggggaaatg attgaatatt cggaggcggt
     4141 ctaccatacg gagctgcatc cttaccatat tgattatgaa acataaataa aaaaagaggt
     4201 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
     4261 tttttcgttt ggttaggctt attaccgcag atacacggct gtttctggat taccatttat
     4321 tctggataag ggggtgtggc acgattttca gaaggaatct atgaaccaat cactgtattg
     4381 attttagctt cgatggtaac cccattcggc ttgacgatcg cttatttctt agggaaaatc
     4441 attcgtaaaa atatactcaa tcgtcaagaa atcgatacat tgaagaccgc ttttcctatg
     4501 gggatctgtc agatcacaga gggatgtttc cctatcgtat tgaacgactt ggcacgcaat
     4561 gtcatagcaa cgggagtcgg tgggacagta ggtggcggat taagcatgtt ttggggcgca
     4621 gatagccgta ttccggcatc gggaatgttt gcagtcgcta cgatgaccag accatgggca
     4681 ttcattggtg cattgttagc tggttctttt gtcacaggaa taaccatttt attattgaaa
     4741 aaaccagtag atccgaatgc agaaatcatc caagaagaaa aagaggaaga agacatatct
     4801 tgggatgatt tgacaattag ttaggagagg cagaatggat tacaagaaaa aagtacaata
     4861 gatgtacctt gagacaggaa tggtatgaaa gaatcagagt tagaaagaat cgaatataca
     4921 gattttggtt taggagcgat cgaaactgtc cagagcatcg gcaccctcct tgaaatgacg
     4981 atgaaggaaa aatgagaacc tttcgttgtc gaaagggtct ggtatacctt atgtcgaagg
     5041 aaaggaaaca aaaaaacttt cctgtactat tccaaaaagg aaagaaagtt attatacagt
     5101 aaaaaaagaa attattcttc atgctagaga tcagtacacg ttcgagccca atatcaaaca
     5161 ctagttccag gcaggcgcaa caggtgcggt tatcactgaa ttttcttcca gcagtgacga
     5221 ttcaagtgat atttttacaa atccagctat ttatcgttaa caaaaagatt tttaggagaa
     5281 gctgatgaac aaaaatgatc aaattttgat cgtttagttc gtcggctttt ttatttagag
     5341 aaacatcatt tatcagtgaa acataacacg agaacaaaat gattttttcg ttaataaaag
     5401 aaacaagtat ttgttgttta aaataataca cgttattggc atataggacg aaatcccagt
     5461 atcttctata agtaagtcat ttcatatcga tgaaaacacg ttatttattt ttgttttatc
     5521 tataa
//
LOCUS       DAZKAO010000147         5516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000147 DAZKAO010000000
VERSION     DAZKAO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.148"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..2132
                     /locus_tag="ACG8H8_002506"
     CDS             72..2132
                     /locus_tag="ACG8H8_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP8987528.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2215..2784
                     /locus_tag="ACG8H8_002507"
     CDS             2215..2784
                     /locus_tag="ACG8H8_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987529.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..3765)
                     /locus_tag="ACG8H8_002508"
     CDS             complement(2959..3765)
                     /locus_tag="ACG8H8_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987530.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3903..5417
                     /locus_tag="ACG8H8_002509"
     CDS             3903..5417
                     /locus_tag="ACG8H8_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP8987531.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1961 a          954 c         1125 g         1476 t
ORIGIN      
        1 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
       61 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      121 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      181 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      241 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      301 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      361 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      421 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      481 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      541 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      601 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      661 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      721 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      781 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      841 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      901 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
      961 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1021 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1081 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1141 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3601 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3661 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3721 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3781 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3841 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3901 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     3961 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4021 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4081 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4141 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4201 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4261 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4321 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4381 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4441 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4501 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4561 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4621 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4681 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4741 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4801 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4861 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4921 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     4981 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5041 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5101 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5161 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5221 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5281 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5341 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5401 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5461 gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaag
//
LOCUS       DAZKAO010000148         5333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000148 DAZKAO010000000
VERSION     DAZKAO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.149"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1222)
                     /locus_tag="ACG8H8_002510"
     CDS             complement(83..1222)
                     /locus_tag="ACG8H8_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8987532.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1531..2244)
                     /locus_tag="ACG8H8_002511"
     CDS             complement(1531..2244)
                     /locus_tag="ACG8H8_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP8987533.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2498..3199)
                     /gene="nagB"
                     /locus_tag="ACG8H8_002512"
     CDS             complement(2498..3199)
                     /gene="nagB"
                     /locus_tag="ACG8H8_002512"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP8987534.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3433..4965
                     /locus_tag="ACG8H8_002513"
     CDS             3433..4965
                     /locus_tag="ACG8H8_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8987535.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(5156..>5333)
                     /locus_tag="ACG8H8_002514"
     CDS             complement(5156..>5333)
                     /locus_tag="ACG8H8_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8987536.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1757 a         1082 c          893 g         1601 t
ORIGIN      
        1 attttaattc aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg
       61 tacactgaac tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta
      121 acttttccga aacatgttcc attcttctca ataaatagat tttccggata tttaatatta
      181 tcgtcttaat cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac
      241 ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa
      301 ataacaacgg cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca
      361 tatacaaagc ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa
      421 taaaagggcc gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc
      481 tataaggctg atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat
      541 ctaagatcgt actgctgata taggcagata atgtttcgtt taattctttt accgtttggc
      601 gtaactcttt acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc
      661 catccttgaa cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca
      721 tcataaatga aatgattttt gagacacttg cagataaact gactaatatg atattgaaca
      781 aattactaag agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact
      841 cttcccgctg cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc
      901 tctgggtcaa ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa
      961 caactaaaat gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat
     1021 gcttttcgag aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca
     1081 atggtaaaaa aattgccgaa agcattttca atattggact aaaaatattc ggcatttgta
     1141 aaataaagta cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg
     1201 attgtttaaa acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc
     1261 atttaacaaa gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa
     1321 catattttaa tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa
     1381 ttatactgca gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa
     1441 gcccagccaa acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg
     1501 taggacatct attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt
     1561 tgtgatattt ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg
     1621 aagatcaatc gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa
     1681 attcacatct tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt
     1741 ttcttttcta tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg
     1801 tttcactact gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta
     1861 acccatgccg atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag
     1921 tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact
     1981 caccacttga gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata
     2041 ctctaaagca tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca
     2101 tgctcccata caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg
     2161 ttcaaaaccg attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg
     2221 aattgtcaat accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa
     2281 ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt
     2341 ttgtcatagt cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca
     2401 acaaacggca aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa
     2461 cgctgatttc acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt
     2521 catcgatgat cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg
     2581 ttacaggtcc ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca
     2641 agacgatttc tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta
     2701 catcttctgc tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa
     2761 ctactgaagt cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt
     2821 gcccgattcc taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt
     2881 tgcatgctgc ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt
     2941 ttttatcaaa taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta
     3001 gtccaacgta ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag
     3061 tcatttcttt atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac
     3121 ctttgttcat accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt
     3181 ttacacgaat gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc
     3241 attctttttt tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt
     3301 gacaataaat aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt
     3361 gcatgaaaac caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa
     3421 aaaggagtta ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa
     3481 atcctctatg aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga
     3541 caaaacggag ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat
     3601 gacggacaga tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa
     3661 gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa
     3721 aaggaagaaa aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg
     3781 ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac
     3841 accttagttg ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg
     3901 ttgaaaaatc taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa
     3961 aacccagatg ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa
     4021 tggctgacgc agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa
     4081 gaatttcttg atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag
     4141 tatactggcc atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg
     4201 cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac
     4261 aaagccggtt cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa
     4321 cggctgacgc ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt
     4381 gtcacgacat tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt
     4441 gcacccatca atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg
     4501 attggaaaat ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat
     4561 ttccacttcc cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat
     4621 ccattagaaa caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa
     4681 ttacgtcgcc aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact
     4741 acattaagtg gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt
     4801 aatttactga ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta
     4861 caagacggga tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt
     4921 tatcaagatc ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata
     4981 gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt
     5041 ggccaattag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt
     5101 tatttggcta ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt
     5161 ttttattttc gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc
     5221 cttgaaaaac aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg
     5281 tccctagcaa tgaaccagcc gcacctccaa taaagtacat aaacataaat aca
//
LOCUS       DAZKAO010000149         5203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000149 DAZKAO010000000
VERSION     DAZKAO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.151"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..721)
                     /locus_tag="ACG8H8_002515"
     CDS             complement(59..721)
                     /locus_tag="ACG8H8_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8987537.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(731..1546)
                     /locus_tag="ACG8H8_002516"
     CDS             complement(731..1546)
                     /locus_tag="ACG8H8_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8987538.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1808..2251)
                     /locus_tag="ACG8H8_002517"
     CDS             complement(1808..2251)
                     /locus_tag="ACG8H8_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP8987539.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2385..2723)
                     /locus_tag="ACG8H8_002518"
     CDS             complement(2385..2723)
                     /locus_tag="ACG8H8_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8987540.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2711..3088)
                     /locus_tag="ACG8H8_002519"
     CDS             complement(2711..3088)
                     /locus_tag="ACG8H8_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8987541.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3312..4505
                     /locus_tag="ACG8H8_002520"
     CDS             3312..4505
                     /locus_tag="ACG8H8_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8987542.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4671..5093)
                     /locus_tag="ACG8H8_002521"
     CDS             complement(4671..5093)
                     /locus_tag="ACG8H8_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987543.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1579 a          987 c          891 g         1746 t
ORIGIN      
        1 aatgacagtt tttacataaa aactgtcatt cttaattgaa agtttcaatt tataaagtct
       61 acagatccat agttctcatc aatttacgat tgttgaagta aatcaaacca aatacgatag
      121 aaggacctag tagaatacac cattggggct ttacgaaaaa gaagagaaac aaacttgtca
      181 atagtaaaaa atctagtaaa ctcacatacc attttttcaa cgtatagtag aacgctatcc
      241 gtagaacttg atttggtttc gacgctggat ttctcacctg aaaataccag tagttgatgc
      301 taaaggaaac tgataaacaa gcaagtaaaa caaacaacgg aatcaaccat tttccataag
      361 aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac actaactatc caagcaaata
      421 gccaataaaa aaatcccttt agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg
      481 gtgaaaaact tttttctttt gctaattctt ctgtcatacc tatcatcacg atgcaacctg
      541 gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct atcgacaaaa
      601 ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta cttgccaatg
      661 attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc tgctctttca
      721 taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa tatttttgca
      781 tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc atgattaatg
      841 cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt gttttatttt
      901 ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta acgaatgtga
      961 tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg cgatagattc
     1021 cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg atactactgt
     1081 agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc accaacgacc
     1141 acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc atataagact
     1201 gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat
     1261 ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc aacgtgatca
     1321 caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga gaccaaataa
     1381 ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc gtgaacactt
     1441 cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc gtcaataaag
     1501 ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt tttgaacgaa
     1561 tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca tttgtttgta
     1621 atgattacaa tccagaacaa cggttattat agctgattca tttccgttct tcttatgttt
     1681 gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt aaaaatgaaa
     1741 aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct tcgattagag
     1801 atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg actacaggtg
     1861 ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt gggtcaagcg
     1921 gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact aaagcagcac
     1981 ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt cctgtgatca
     2041 gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt tctggacgct
     2101 ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct ttttttgcta
     2161 aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac aaaatcgttt
     2221 cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg cttttttcta
     2281 gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg ctgggataat
     2341 cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct tccacacagc
     2401 cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct tgagtggttt
     2461 gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc tgcttctgtc
     2521 aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc ttctaactcc
     2581 ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc ttgagagatt
     2641 gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat agcgatgctc
     2701 cttgattttt ttacttgata cattccattt tatttctaga taacacacga tcatatttct
     2761 tgatttttcc ttgtaaacgt tccaaacatt cttgcatttc agcaattttt ttcgtcaaaa
     2821 gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct ccttcgctag
     2881 ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc atacagatcg
     2941 taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct tttgtccgat
     3001 taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc gaaatgccct
     3061 tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt tcttgccact
     3121 ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa tactcatttc
     3181 tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca aataaaaaag
     3241 tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat tttaggaaaa
     3301 ggggtataaa aatgacaacg tatcagcgaa agttattatt atcgacatcg gcgggaattg
     3361 ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg attacttctt
     3421 ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg gggatgctca
     3481 ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa gtattttctc
     3541 aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca aacatctact
     3601 tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac ggggcgtgta
     3661 tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct tcggttgctg
     3721 gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg atcattccaa
     3781 cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca
     3841 ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa
     3901 ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt ggccttagta
     3961 tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg ccttcaatta
     4021 tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc agtactattt
     4081 taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg gggcctaaac
     4141 tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt gtgttagccc
     4201 atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc aatggcatgt
     4261 atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc gcgacggcga
     4321 ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc gtcatcggtt
     4381 tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt atttacctag
     4441 tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa agcttgcagc
     4501 attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg catctgcggt
     4561 aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg tcgtattact
     4621 tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcta ctattctttg
     4681 acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac tgtggataca
     4741 ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg atacatgaat
     4801 tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat gtagattggg
     4861 aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa aatgacctta
     4921 tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat agacttactt
     4981 gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt ttcaagcagt
     5041 aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg catattttct
     5101 cctcctgtat ctactttact atactacata caagaaaaaa aacggataca aatatcatat
     5161 tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctt
//
LOCUS       DAZKAO010000150         5041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000150 DAZKAO010000000
VERSION     DAZKAO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.152"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..2005
                     /locus_tag="ACG8H8_002522"
     CDS             71..2005
                     /locus_tag="ACG8H8_002522"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8987544.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2462..2878
                     /locus_tag="ACG8H8_002523"
     CDS             2462..2878
                     /locus_tag="ACG8H8_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP8987545.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2892..3458
                     /locus_tag="ACG8H8_002524"
     CDS             2892..3458
                     /locus_tag="ACG8H8_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP8987546.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3481..4467
                     /locus_tag="ACG8H8_002525"
     CDS             3481..4467
                     /locus_tag="ACG8H8_002525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP8987547.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4622..>5041)
                     /gene="scm"
                     /locus_tag="ACG8H8_002526"
     CDS             complement(4622..>5041)
                     /gene="scm"
                     /locus_tag="ACG8H8_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP8987548.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1536 a          929 c         1106 g         1470 t
ORIGIN      
        1 taataagtat caaactaaaa gaattatcat atattcatta tatagatagt ctataaggag
       61 cggatgatca atggcaaagc aaaatattgt cgttgtagga gcaggatatg ccggtgtttc
      121 agctacaaaa tacttggcaa agaaactgaa aaaagaagat gtagcaatta ctctgatcga
      181 tcgtcattcg taccatacta tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga
      241 accagaagca atccaatacg atctgcagca tttgtttgct cgtcaaaaga atgtacaact
      301 tgtcacagac actgtaatcg gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg
      361 ttcctatcct tttgatcagc tgatccttgg aatgggcgga gagccgaatg actttggtac
      421 acctggtgtc aaagcacacg gattcaccct ttggtcattt gaagattctg taaggattcg
      481 ggaacatatt gagaagacag tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa
      541 agccatgctg actttcgttg tctgtggttc tggatttacc ggtattgaaa tgattgggga
      601 actgatcgac tggaaaaatc gcctagccaa agattataaa ttagatccaa acgaaatcac
      661 actgatggtc gtagaggcaa tgccgacgat cttgaatatg ttggatcgaa atgatgctgc
      721 aaaagctgaa cgctatttgc gtaagcacaa cgtggaatta ttattgaatg caccaatcgt
      781 agaagtggct gctgatcata tcaaattgaa agacggacgc aacattccga ctcacacact
      841 gatttggaca gctggtgtca aaggaacttc tgacgctgct gactttggat tagaatcagc
      901 acgcggacag cggttgatcg ccaatgaata tatgcaggca aaaggctacg aagataaaaa
      961 catctatatc attggtgatt tagtctttta tgaagaattc ccaggaacac cgacaccgca
     1021 gatcgttcaa gctgctgaac aaacaggaca cacagccgca gcgaatatcg tggcttcgat
     1081 caaaggcgga gaaaagcata aatttaaagg aaactatcaa ggatttatgg tttctattgg
     1141 tgccaaatgg ggggtagcta acctatttga taagatccat ttaagcggat ttttagctat
     1201 cgtaatgaaa catatcgtga atttgaaata cttctttgat attcgttctg gttattatat
     1261 gttccaatat ttaatgcatg agtttttcca tatcaaagat gatcgcaacg tgactcgagg
     1321 acattcttct cgttacggca atgtcttatg gagtgtgcca cttcgtattt tctatggttt
     1381 agtttggttc gttgaagctt ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag
     1441 tacttggttt ggtgaaggat catggttcac tggaaacagt gtgttccctt ggacatggga
     1501 atacaatgta acagatgttg ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc
     1561 cagcggagca ggggcaagtg ccggagaagc agcgactcaa gctgcacatt ttggtttgag
     1621 ttatgcatac ggtgaagaac caatggctgt gattgagaag tcaccagact ggttcacaaa
     1681 gatcatgcaa gtgatcatgc caaacaatga tgtagcaatg ttcttccaaa aattcatggt
     1741 attctttgaa attgccttgg cattagcatt gattgctgga ttattcacat ggctatgcag
     1801 tgcgacaacg attgtattgg tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa
     1861 catttggttc atttttgttg ctttcgcatt gatgaacggt tccggacgtg cgattgggtt
     1921 ggatcgctgg gtaattccat ggctacaacg tacactaggt aattggtggt acggtagacc
     1981 aagttcccag tacggcaaga aataaataga taaagaacgt tatttgaatg caagatttca
     2041 atagaaaggg gctgagacaa accgtcacag cctccttttt atgtaaaatt aaaggaataa
     2101 gatgcccgag ataagcctcc atgagcaaga cacaggtgtc acaacctctg ttacaacctc
     2161 ttgttataaa cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa
     2221 acttgaaaag taattttcgt aaattccttc ttatttcttg aagttgccca cttctgtcac
     2281 aacctcttgt tataaacggt gttccacctg ctgcttctcg gtaataagcc caaagaatta
     2341 gaaaacatgg aaagctgttt tctattcttc gttcttatta ctcaaagcaa gacacaggtg
     2401 tcacaacctc ttgtttttat gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa
     2461 aatgagcctt aaagaatttg ctgaaaaaag tctgctcaaa ccttgggatg tttttattat
     2521 tttgcttttg actgttcttt cttttttacc agtcatgatt tttggtcttc agcaaaacac
     2581 cgcaacagca gaaaaagaag cggtactgcg tgtggatggc acagaaatca aaacattccc
     2641 tttgatggct ggagaagaga gcaacacata tacttacaaa gatgaacacg gagattataa
     2701 cttgatcgag atagatggcg atcgagtacg gataaaagaa gcagattgcg atgaccagat
     2761 ctgcgtacgt cgaggctggg caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa
     2821 attagtgatc gaagtccgtt caacagatgg aggtgacgaa gacagtctga tttattagac
     2881 ctttacacac catgagtaaa ttacaaaaaa tgatttatat ttcgctactt gttgcacaag
     2941 gagtgatcat tggtttattg gaaaatatga tcccttatcc tttttctttt gcgcctggtg
     3001 caaaactagg cttagccaat ctaatcacga tcgttgccat ttttaccatg aaaaaacaag
     3061 acagcttctt actactttgg ctacgactat tcctgactac tttgctaggc ggaacgattt
     3121 ccacattcct ttatagtatg agtggtgctt tgcttagtta ttttggaatg ttattggtca
     3181 agcaattagg accaaaacgt gtcagtatca tcggaattag tgcggccggc ggtttcatgc
     3241 ataatgtcgg acagcttttg atggcttctt tcattgcaca atcatggaca gtcatgttgt
     3301 acttgccagt cctttcagtt ttagggattt tatcagggat tgcaatcggg atcgcagcga
     3361 attatctttt acgacatgtg cagacacttc aacgattcca aaaagattat gagcagggaa
     3421 ccaaacaaaa atggagccca atgtttacca aaaaataatc gtcacacaag gagttttttt
     3481 atgaagctac atccaatgtg ggaaaactat cctgctttaa gtaaagaatt gacagaaaca
     3541 ctaaaacgga tggaagaaac tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa
     3601 gaaacgatcc atgccggcgg aaagctgttg cgtcctgcct atcagctatt gttctcacaa
     3661 tttggaccag agcaagatcg agacaaagcc atcgctttgg cagcttctat cgaaatgctc
     3721 catacggcca cactgatcca tgatgatatc gttgatgaag cggatctgcg acgtggtcag
     3781 cctaatatcc gtagtcagtt tggtaatgtc gtttctgtct atgcaggaga ctatttattt
     3841 gtctgctgtt ttaaactatt gtctgattac tccacttcct tgaaaagtct ccaattgaat
     3901 tctcgcagta tggaaaaagt attgaatggc gaattagggc agatggatga tcgctataat
     3961 tatgaacttt ctgtaaaaga atatttggaa aatatttcag gtaagacagc tgaactgttc
     4021 caattaagtt gttctgttgg cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa
     4081 gcaggagata tcggactttc tatcgggatg gcctttcaga ttatcgatga tattttagat
     4141 tatacaaaag agagccagga aatcggcaaa cctgtactag aagacatgcg tcaaggggtc
     4201 tattctttac cgttgatcta ctctttgcaa aaaaacaaac caaagctact accttattta
     4261 gagaagaaag cggcaatgac agaagaagat gtcgacgccg tacgcaaaat cgttgaacat
     4321 acaggaggcg tggaagaagc taggaagtta gcggcctctt acacagaaaa ggctttgaag
     4381 gaaataaaga aattacctgc cacctctctt cgaacaaaag agaatctctt ttcccttacc
     4441 caactgattt tggatcgcaa agactgaata gggaaaggtt cgatacgtaa ataaatttct
     4501 cagaaggaaa tctacgtgtt ttttgacaaa ttgaattgta gttcttgtat ttatgataaa
     4561 aagcaaaaat aaagagcaac gtccgttagc gacgttgctc ttttttatct tgaatatttt
     4621 tttacctttt cgatttctta taactcataa aagtgattct taaaccgatt acaagtaata
     4681 taacgcctgc gagtgttatc caattattcg attgacttcc agttttagga agtgctcttt
     4741 ttgatttgtt tccattgtca ttgcttgttt tattagttaa gttaaagttc gtcctgttat
     4801 ccgaacttgt ttttgagaag gagacacttg catgacgtgt atcgcttgtg gtagacgacg
     4861 tgtctgtact atcgcttgaa gttgatgaat ctgagctatc acttgtggta gaggatgtat
     4921 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgtagtggta gatgaatctg
     4981 agctatcact tgtggtagag gtagtatctg tactgtcact tgaagctgac gtgtccgtgc
     5041 t
//
LOCUS       DAZKAO010000151         4973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000151 DAZKAO010000000
VERSION     DAZKAO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.153"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..447
                     /gene="mgsA"
                     /locus_tag="ACG8H8_002527"
     CDS             25..447
                     /gene="mgsA"
                     /locus_tag="ACG8H8_002527"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP8987549.1"
                     /translation="MKIALIAHDRKKTMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      634..732
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            770..1306
                     /locus_tag="ACG8H8_002528"
     CDS             770..1306
                     /locus_tag="ACG8H8_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP8987550.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1346..3109)
                     /locus_tag="ACG8H8_002529"
     CDS             complement(1346..3109)
                     /locus_tag="ACG8H8_002529"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987551.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3112..4827)
                     /locus_tag="ACG8H8_002530"
     CDS             complement(3112..4827)
                     /locus_tag="ACG8H8_002530"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987552.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1515 a          969 c         1008 g         1481 t
ORIGIN      
        1 acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa
       61 actatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt
      121 gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt
      181 aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta
      241 gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca
      301 gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct
      361 gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa
      421 atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt
      481 tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt
      541 attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc
      601 tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt
      661 gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc
      721 gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca
      781 ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt
      841 gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca
      901 agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga
      961 tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc
     1021 atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa
     1081 tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg
     1141 gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt
     1201 aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat
     1261 tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct
     1321 tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc
     1381 ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca atagctccct
     1441 gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa
     1501 aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg
     1561 tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc
     1621 gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct
     1681 tagtatctaa tttttccggc aaacgttcta caaattcata catataagag gcttttaggg
     1741 catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg
     1801 tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac
     1861 gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa
     1921 aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa
     1981 tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat
     2041 aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat
     2101 aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta
     2161 gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag
     2221 aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac
     2281 tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa
     2341 aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata
     2401 gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca
     2461 cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt
     2521 gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa
     2581 ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa
     2641 acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa
     2701 tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat
     2761 cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa
     2821 cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga
     2881 ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc
     2941 cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa
     3001 tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt
     3061 aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc
     3121 ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt
     3181 accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga
     3241 gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg
     3301 caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc
     3361 tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt
     3421 tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc
     3481 tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca
     3541 ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat
     3601 ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt
     3661 accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc
     3721 ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag
     3781 agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc
     3841 agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag
     3901 cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga
     3961 aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc
     4021 ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc
     4081 gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa
     4141 ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg
     4201 cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg
     4261 atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa
     4321 aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac
     4381 aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac
     4441 cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc
     4501 tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt
     4561 acgtaactct gtaccaaatc cttgggaagc tactgaagca tagtattggc aaatgatagc
     4621 acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc
     4681 tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag
     4741 ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc
     4801 ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt
     4861 gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc
     4921 ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga cta
//
LOCUS       DAZKAO010000152         4952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000152 DAZKAO010000000
VERSION     DAZKAO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.154"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="ACG8H8_002531"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="ACG8H8_002531"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP8987553.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="ACG8H8_002532"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="ACG8H8_002532"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP8987554.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="ACG8H8_002533"
     CDS             2592..3377
                     /locus_tag="ACG8H8_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8987555.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="ACG8H8_002534"
     CDS             3390..4241
                     /locus_tag="ACG8H8_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP8987556.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="ACG8H8_002535"
     CDS             complement(4365..4817)
                     /locus_tag="ACG8H8_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987557.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1677 a          810 c         1034 g         1431 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg aaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa ga
//
LOCUS       DAZKAO010000153         4931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000153 DAZKAO010000000
VERSION     DAZKAO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.155"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8H8_002536"
     CDS             85..972
                     /locus_tag="ACG8H8_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8987558.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8H8_002537"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8H8_002537"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP8987559.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8H8_002538"
     CDS             2274..2720
                     /locus_tag="ACG8H8_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8987560.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8H8_002539"
     CDS             2993..4345
                     /locus_tag="ACG8H8_002539"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP8987561.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8H8_002540"
     CDS             4516..4803
                     /locus_tag="ACG8H8_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP8987562.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAZKAO010000154         4907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000154 DAZKAO010000000
VERSION     DAZKAO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.156"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1055)
                     /locus_tag="ACG8H8_002541"
     CDS             complement(93..1055)
                     /locus_tag="ACG8H8_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8987563.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="ACG8H8_002542"
     CDS             complement(1057..2496)
                     /locus_tag="ACG8H8_002542"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP8987564.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="ACG8H8_002543"
     CDS             2681..4627
                     /locus_tag="ACG8H8_002543"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP8987565.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1586 a          816 c          795 g         1710 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaatga aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa
     4741 aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac
     4801 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
     4861 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaat
//
LOCUS       DAZKAO010000155         4884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000155 DAZKAO010000000
VERSION     DAZKAO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.157"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            509..2353
                     /locus_tag="ACG8H8_002544"
     CDS             509..2353
                     /locus_tag="ACG8H8_002544"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8987566.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="ACG8H8_002545"
     CDS             2409..3875
                     /locus_tag="ACG8H8_002545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8987567.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="ACG8H8_002546"
     CDS             3912..4754
                     /locus_tag="ACG8H8_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8987568.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          785 c         1028 g         1466 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atct
//
LOCUS       DAZKAO010000156         4871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000156 DAZKAO010000000
VERSION     DAZKAO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.158"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8H8_002547"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8H8_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP8987569.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8H8_002548"
     CDS             724..1878
                     /locus_tag="ACG8H8_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP8987570.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8H8_002549"
     CDS             2093..3061
                     /locus_tag="ACG8H8_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP8987571.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8H8_002550"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8H8_002550"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP8987572.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8H8_002551"
     CDS             4091..4699
                     /locus_tag="ACG8H8_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP8987573.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="ACG8H8_002552"
     CDS             complement(4779..>4871)
                     /locus_tag="ACG8H8_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP8987574.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DAZKAO010000157         4864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000157 DAZKAO010000000
VERSION     DAZKAO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.159"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8H8_002553"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8H8_002553"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP8987575.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8H8_002554"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8H8_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987576.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8H8_002555"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8H8_002555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8987577.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8H8_002556"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8H8_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987578.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8H8_002557"
     tRNA            3917..4000
                     /locus_tag="ACG8H8_002557"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4864)
                     /locus_tag="ACG8H8_002558"
                     /pseudo
     CDS             complement(4240..4864)
                     /locus_tag="ACG8H8_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1487 a         1012 c          780 g         1585 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aact
//
LOCUS       DAZKAO010000158         4736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000158 DAZKAO010000000
VERSION     DAZKAO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.161"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1101)
                     /gene="xerS"
                     /locus_tag="ACG8H8_002559"
     CDS             complement(16..1101)
                     /gene="xerS"
                     /locus_tag="ACG8H8_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP8987579.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1450..1645
                     /locus_tag="ACG8H8_002560"
                     /pseudo
     CDS             1450..1645
                     /locus_tag="ACG8H8_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3177..3695)
                     /locus_tag="ACG8H8_002561"
     CDS             complement(3177..3695)
                     /locus_tag="ACG8H8_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP8987580.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4062..4310
                     /locus_tag="ACG8H8_002562"
     CDS             4062..4310
                     /locus_tag="ACG8H8_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987581.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1555 a          778 c          718 g         1685 t
ORIGIN      
        1 tattttttac tacatttaca aagcgttcaa agcatcaatt gtagctgcat tgtctatatg
       61 agtatataaa gttgttgtat ttgtacttgt atgtcccaat tgttgggcag ttaataccaa
      121 tgaatttgtc tgttgataga gtctagtagc caaagtatga cgtaatttat gtggactgac
      181 ttggactctg aatccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
      241 acgcttggct tctccttgat aaattgctag aaataaagct ttttcgtttt ctgttacttt
      301 gtatcgttct ttccgaatag ctagataatt agctaaatat tccattgcaa atgctgctat
      361 atttactgcg tctctcttat ttccttttct agttactacc acattattct tttctaaaga
      421 taggtcctct aaattaatat ttacaacctc ggagagtctt aatccagatc ctaaaatcaa
      481 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttgct
      541 caaagttttt ttatattcat tggcaatata atctagataa ttttgtgtat cttcgtctag
      601 aaataatttt ttctcgattg cgtttgcacg ggcagctaac gtagttttat ctgtgatagt
      661 tgctacttga tccataggat tattttctaa gtatggtttt cctaaaggat tattggaact
      721 tttcgaaaga tatttaaata gaacctttaa tgctgtgata tttctattga tagtagaagt
      781 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
      841 ttcctttgtg cgacgtttca aatgagattt gtacaagttt agatcatagg cagttaactg
      901 tcttatcgag gtgattgaag tatcgggaac gatagactca ctcagaagcc attcataaaa
      961 gagcttataa ttttttagat attctaaagt agttcgaggt gattgatctt tatcctcacg
     1021 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
     1081 atatatttct gtatttttca tgttattcat cctttcgata agtaggggag aggagtatct
     1141 ctataattgg actataccaa ttatagagtg ttttgtcaat tggtatactc tgaaatttat
     1201 taatttattt caaaaaagca gataaatagg tatagattta ataatttaaa ataaatggta
     1261 tacatgaata ggaaaggaaa cagaaggagg caatcttaat tttttaaatt gtctaaatta
     1321 tatagaaaga acaatctttc tatataatta gacataagag agactacggt tgatttgata
     1381 ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg gagggagaag ttaaagaaaa
     1441 ttcagagaag tggggcactc agtagtatta acaatttaaa aggaatttct taaagaaaat
     1501 gggttgcatc ctggagatac aattttttta gataaagaaa aaattcaagc aacaattcaa
     1561 aaaaatctta actgctgaga ttgatgtagc aatatctcaa tcatcaacaa agtacgatcc
     1621 tgcctttcaa aagcttattg ataaatgttt attcattata aaaataaaag aaaaatttat
     1681 gtcagctttt ttcatcaaag tggtaatgat aattcgtcta ttaattgtat tttttaggaa
     1741 atatggtata attgttttaa cgaaaaggta attcatgata caccaaaaac cactagctgg
     1801 cgggctagtg gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt
     1861 tggcaataat tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga
     1921 ttgcaaaaaa catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag
     1981 gccggggtag cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt
     2041 tcaatatatt gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt
     2101 aaaccagata taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt
     2161 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
     2221 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
     2281 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     2341 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     2401 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     2461 accttttaca tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt
     2521 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     2581 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     2641 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     2701 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     2761 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     2821 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     2881 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     2941 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     3001 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     3061 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     3121 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     3181 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     3241 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     3301 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     3361 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     3421 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     3481 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     3541 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     3601 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     3661 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     3721 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     3781 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     3841 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     3901 tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact
     3961 actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa
     4021 gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc
     4081 tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg
     4141 aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac
     4201 gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg
     4261 ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag
     4321 ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc
     4381 tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta
     4441 aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct
     4501 ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa
     4561 accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa
     4621 acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt
     4681 atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca cttagc
//
LOCUS       DAZKAO010000159         4733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000159 DAZKAO010000000
VERSION     DAZKAO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.162"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8H8_002563"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8H8_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP8987582.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACG8H8_002564"
     CDS             complement(2066..3064)
                     /locus_tag="ACG8H8_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP8987583.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACG8H8_002565"
     CDS             complement(3290..3943)
                     /locus_tag="ACG8H8_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP8987584.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8H8_002566"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8H8_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP8987585.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1389 a          995 c          742 g         1607 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atc
//
LOCUS       DAZKAO010000160         4679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000160 DAZKAO010000000
VERSION     DAZKAO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.163"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..382
                     /locus_tag="ACG8H8_002567"
     CDS             152..382
                     /locus_tag="ACG8H8_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP8987586.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            479..1780
                     /gene="murA"
                     /locus_tag="ACG8H8_002568"
     CDS             479..1780
                     /gene="murA"
                     /locus_tag="ACG8H8_002568"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP8987587.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1793..1969
                     /locus_tag="ACG8H8_002569"
     CDS             1793..1969
                     /locus_tag="ACG8H8_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP8987588.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2104..2400
                     /gene="yidD"
                     /locus_tag="ACG8H8_002570"
     CDS             2104..2400
                     /gene="yidD"
                     /locus_tag="ACG8H8_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP8987589.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2584..3051
                     /locus_tag="ACG8H8_002571"
     CDS             2584..3051
                     /locus_tag="ACG8H8_002571"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987590.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3136..4020
                     /locus_tag="ACG8H8_002572"
     CDS             3136..4020
                     /locus_tag="ACG8H8_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8987591.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4134..4370
                     /locus_tag="ACG8H8_002573"
     CDS             4134..4370
                     /locus_tag="ACG8H8_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP8987592.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4458..4538
                     /locus_tag="ACG8H8_002574"
     tRNA            4458..4538
                     /locus_tag="ACG8H8_002574"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4492..4494,aa:Tyr,seq:gta)
     gene            4545..4616
                     /locus_tag="ACG8H8_002575"
     tRNA            4545..4616
                     /locus_tag="ACG8H8_002575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4577..4579,aa:Gln,seq:ttg)
BASE COUNT         1570 a          732 c         1023 g         1354 t
ORIGIN      
        1 gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata
       61 tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta
      121 ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct
      181 tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg
      241 cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt
      301 ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg ctttatgccg
      361 aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt
      421 ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat
      481 ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc
      541 taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac
      601 attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt
      661 aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg cttcaagaca
      721 attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat
      781 gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat
      841 tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat
      901 tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt
      961 agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg
     1021 aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt
     1081 gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt
     1141 ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt
     1201 tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca
     1261 taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg
     1321 cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca
     1381 tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg
     1441 tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg
     1501 ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga
     1561 attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc
     1621 tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata
     1681 ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg tagacatgga
     1741 tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa
     1801 ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct
     1861 tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa
     1921 agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat
     1981 cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa aagaaaacag
     2041 aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa
     2101 ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca
     2161 tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa
     2221 acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct
     2281 tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg aaataagaaa
     2341 gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa
     2401 agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat tgccgaagtg
     2461 ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac tgagcgataa
     2521 agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata tgggggacaa
     2581 aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat
     2641 cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa
     2701 aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc
     2761 aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca
     2821 gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat acgaaccggc
     2881 agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga gattggtcga
     2941 gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta
     3001 agggatcaag taattttagc aattcactta cctaaaaata gctttaacta ataaactttc
     3061 aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa
     3121 aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat
     3181 ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg
     3241 gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc
     3301 gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga
     3361 atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt
     3421 caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa
     3481 ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg
     3541 gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg agaatttggg
     3601 ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt acagatggct
     3661 tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa agatgtcttt
     3721 gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat
     3781 cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc
     3841 ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg
     3901 acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga atacagaaac
     3961 gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg ggagaattaa
     4021 gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata ggagattttt
     4081 acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt
     4141 caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta
     4201 agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct
     4261 atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc
     4321 ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca
     4381 aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag
     4441 gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg
     4501 ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc
     4561 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata
     4621 ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct accttgttc
//
LOCUS       DAZKAO010000161         4667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000161 DAZKAO010000000
VERSION     DAZKAO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.164"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACG8H8_002576"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACG8H8_002576"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8987593.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="ACG8H8_002577"
     CDS             complement(1347..1982)
                     /locus_tag="ACG8H8_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP8987594.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="ACG8H8_002578"
     CDS             complement(2248..3300)
                     /locus_tag="ACG8H8_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8987595.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="ACG8H8_002579"
     CDS             complement(3313..4251)
                     /locus_tag="ACG8H8_002579"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8987596.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1516 a          985 c          717 g         1449 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttac
//
LOCUS       DAZKAO010000162         4610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000162 DAZKAO010000000
VERSION     DAZKAO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.165"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG8H8_002580"
     CDS             complement(12..482)
                     /locus_tag="ACG8H8_002580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP8987597.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG8H8_002581"
     CDS             complement(641..1099)
                     /locus_tag="ACG8H8_002581"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP8987598.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG8H8_002582"
     CDS             complement(1270..2415)
                     /locus_tag="ACG8H8_002582"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP8987599.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG8H8_002583"
     CDS             complement(2428..3345)
                     /locus_tag="ACG8H8_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP8987600.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG8H8_002584"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG8H8_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP8987601.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACG8H8_002585"
     CDS             4437..4607
                     /locus_tag="ACG8H8_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987602.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1442 a          844 c          870 g         1454 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata
//
LOCUS       DAZKAO010000163         4596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000163 DAZKAO010000000
VERSION     DAZKAO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.166"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..829)
                     /locus_tag="ACG8H8_002586"
                     /pseudo
     CDS             complement(1..829)
                     /locus_tag="ACG8H8_002586"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(1089..1637)
                     /locus_tag="ACG8H8_002587"
     CDS             complement(1089..1637)
                     /locus_tag="ACG8H8_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987603.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1638..2495)
                     /locus_tag="ACG8H8_002588"
     CDS             complement(1638..2495)
                     /locus_tag="ACG8H8_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP8987604.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2781..3068)
                     /locus_tag="ACG8H8_002589"
     CDS             complement(2781..3068)
                     /locus_tag="ACG8H8_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8987605.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3058..3387)
                     /locus_tag="ACG8H8_002590"
     CDS             complement(3058..3387)
                     /locus_tag="ACG8H8_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8987606.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3464..3736)
                     /locus_tag="ACG8H8_002591"
     CDS             complement(3464..3736)
                     /locus_tag="ACG8H8_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP8987607.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3736..3999)
                     /locus_tag="ACG8H8_002592"
     CDS             complement(3736..3999)
                     /locus_tag="ACG8H8_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8987608.1"
                     /translation="MAKTVLNPSTARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4105..>4596)
                     /locus_tag="ACG8H8_002593"
     CDS             complement(4105..>4596)
                     /locus_tag="ACG8H8_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987609.1"
                     /translation="ITSNKTSRVLLALMGYGILYRDDQNGQIIITRAGRSLLMKYRKF
                     IPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDANH
                     MYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKNK
                     GNG"
BASE COUNT         1346 a          947 c          582 g         1721 t
ORIGIN      
        1 taattaatag cttcagttaa gattgccaaa ctggtacctc cactactacc aaatacacgt
       61 ataggtacca tcgtaacatc ccaatttatt ccagcaacac ctaattcgtt gtttgtttga
      121 gcaccaataa tcccggcaat atgtgtccat gtcccatagt atccatagga tctgtatcat
      181 cgttaaaaaa atcgtatctc aaactttcat ctacatttcc agaaagatct tcatgttgat
      241 aatcaattcc tgaatcaatg accgctacct tcacatcctt agaacccttg gtttcttccc
      301 aagcggatgg catgctaatc tctcttaaat tagcttgttt actccaatta ggatcgttaa
      361 gaataattga tcgatttcct aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg
      421 gctctagttc agccacatag tttggctctg cataattgac catatcagaa gcattaaatg
      481 tttttactgc ctcttccaca ctaatttctt cgttcaattt cactaataat acttgatctc
      541 gcttattata atcgctttca tttgtcctaa gagtcaaatc tttttccacc ataactttta
      601 cacccaataa attttgcggt ttatttttct ctgttgaaat tctttgtgta gcctttaaac
      661 tgacaattag ctcattgggt acatactcta tttgagagtc atctaattgg ctcataatcc
      721 tttctttttg gttagtatct gcagttactt gtttagtgtg agccacgaac ccaaacatac
      781 tcaccccaac aaccaatcct aaatatattt tctttttaaa ctttttcatc tacatctcct
      841 cctaatagtt ttattttaga gttagctcta ttacttattt actaactcaa caacagatta
      901 tcatacactt gttaatgaaa ttacaatatc tcattaaaac acaccctaaa atcataaccg
      961 ttcctatgct cattttttgg attttttaat cacgtaacca gaaaaataaa tcctctctat
     1021 ttcacgcaaa tcaaagaaga actaagtaac taccgtttaa aggtagttac ttagttcttc
     1081 tttgattatt atctaacttt attattcatt ccccaccctt tgcgcagtga gtcttcggta
     1141 tcttgtttta tctgactggt agttcttatt atttctccac gatcatctaa ttctggaaca
     1201 attggatatt tttctctgaa caagtctagt tctttcacca cgtcattaaa caaatcttgc
     1261 ttatcttctt gcgtaagatt ctgtttaata tactcatcta aagcttttct aagcaattca
     1321 tctcttgtca tattagtctc atacttttct actaaaactc tggaagtcgc atttaacaaa
     1381 gtcgacagtt ttaaaggatg cttggtggaa taaaccttat caaactttct gattggtgcg
     1441 ccaacttttt ttgttcttcc tctactctgt ctttcaacat tggaggaagt ttttatttct
     1501 tgaacttgat gcacttctaa ttgcttattt tcagatggtg taattgcttg ctgctttttc
     1561 tttgcttctg cttctttttt tattttggcg agtcctttgt tgttcccaaa aatatcaagt
     1621 cctttgatgt cagccaatta ctccacctcc gcataccaat tctcgtgttc ttccaattct
     1681 ttcaaaatac tgataaataa ttcgtggctt ttgcgatccc attgttttaa gagacctttc
     1741 tttgtcttct ccatcgtaat acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt
     1801 acaacatttt taatcaaatg ttccccataa atctcttggg cttgtttata catttcctga
     1861 tctactgcgt caccgctttc caacatgaaa ggaataacac cagcaacata taaatttgta
     1921 tcaaattctt tcacaaaaaa gtccatatac tcaagatatt gcatcgcatt tctcatcgat
     1981 tttacttggg tctgtaagac gacgattgcc cagtccgcaa tgtacatcgc ggatttacta
     2041 tatacagaaa tagaaggtgg gacatctaaa tagatgacgt caaactcatc aaataatggt
     2101 tctagctttt ctttcatata tttgaattgg gataatgaat cattatattg gtctttgatc
     2161 caatcatcat acatttcaaa ggacatgtct gctggaagta tatagaggtt attgattact
     2221 ttatagacag cctctttagg tggaagattg tttatcaaca cgtcagtcac agtattttcg
     2281 taaacaatcg gttcaccttc tccgcctcct tcttcgttag tgaagttcat atgcgttaag
     2341 ttcatcacat cagaagtatt tccttgcata tccaagtcaa ttatcaaggt tctttttcct
     2401 tcattaactg ctgattcaaa ccctaacatt tgcgcgcacg tacttttacc tacgccacct
     2461 ttaaaattac cgattacata tttcttcgct accacgttcc tacctctttt cttttagcaa
     2521 tattcatttg atactattat aacatattta tttttagtta ctaccaaaaa aaagatatat
     2581 atgtactacc ttataattaa taagtaaaag gtagtacata aataaggaaa agctgaaata
     2641 tattgattta tagctttcct caaagtaatg acgttattta atttatcgag aagcagagaa
     2701 tactcgcgac tttagtcatg tagacgaatc agattttcga taaaaaagaa gccgtccttc
     2761 ttaagaacga caacaaatca ctagattaat tttttcaact gttcataaaa gttttcatgc
     2821 gttccacata acaatactgg aataaccttg ttttctataa tctcgtaagc tactctatat
     2881 gttgtgcccg catatttgaa cccactagac caaatacctg ataaatcccc agttttttgt
     2941 tcacccttat atggatcaat cgcaatttca tcataaatta aattcaaaaa tttttctttc
     3001 aatggcttct cttttagctt ttttaaatac ttaatgacat gttttgaagg aacgatttca
     3061 taagccaatt tctgttgcca actcctcttt agtcattact ttagaattag taagtgtatc
     3121 ttcagcaata tctctaaagg aagaatgcat tttgtttttt ctattcttaa attctacaag
     3181 aatctcatct ttatcgtagc cttctgcaat tatatcagac aaaatatctt cgctaaaatc
     3241 ataaaattct ttttgtggtt caacaaattc ataaagaata ccattctcta ccaactttgc
     3301 ttcaacttcg actccattag gaacattaaa atctttaggt actgtcaaca tgattgaatt
     3361 gccttgtttt cttgtttttg taatcattta aatccctcat ttctaacagt atgtaattac
     3421 ttagtaaata cattatacca cgagataatg tatttacaag taattactgt gactcgtaat
     3481 gcgtccttgc tgaataaatt tcaaccacgt gttcatcttc atctacctta taaacaaccc
     3541 ggtgttgtct attcaatctt ctagaatacc taccttcatg tgtaggtttt aacttctcaa
     3601 atgattgggt agggagatag ggatcttcct ttaaagtctg aataaccttc tcaaattgag
     3661 gtttcaaatt agactgtttg attttcttta aatcagcttt agccgagttt ttaatcatca
     3721 cagaatagtt attcattata agttatccca atcaatttca tcaacattag taaatccact
     3781 atcatctttt tctcgcttac gaacaacacc caaagtgcct atttgctcaa gtaatagcgt
     3841 ttctttgatt gctttccaat catccaaacc aataagcacc gcattatttt cactgcgctc
     3901 actaatgatt tcaatttccg tatgattctc atttacatct ttcaacaact tataaaaatt
     3961 tttccgtgct gtgctaggat ttaagacagt ttttgccata tctactcccc ctctaattta
     4021 tatctccatt ctaacgtact taattaagta cgtaaattcg attgttttgt ttaaattgga
     4081 gtaccaaaac agcattgtaa agtgttatcc gtttccttta tttttattaa caagttttat
     4141 cacaggcttc atatatttga ttatatcttc atcttctaca tagatatctg ttatcattac
     4201 gacatctgta ctatttcgat tgagtacttt cacttgatct ccagcattga ttttccctct
     4261 aaatctttta ggaatgaacc aactatacat atgattagca tcaccaaaat gtgcttttat
     4321 cgcattctca ggatataact tattcaatag ttcattcttt tgaattttca aaacttcaaa
     4381 tctatactta tcatcttctg ctaatctaaa taaatcttct aaacgcatgt tttctaactg
     4441 taagttttga aaatcttccg gtataaattt tctatatttc attaataatg aacgcccggc
     4501 ccgagttatt ataatctggc cattttggtc atctctataa agaattccgt aacccatcaa
     4561 cgccaatagc acacgtgacg ttttgttact tgtaat
//
LOCUS       DAZKAO010000164         4587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000164 DAZKAO010000000
VERSION     DAZKAO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.167"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG8H8_002594"
     CDS             186..2855
                     /locus_tag="ACG8H8_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP8987610.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG8H8_002595"
     CDS             complement(2946..3560)
                     /locus_tag="ACG8H8_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8987611.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACG8H8_002596"
     CDS             3680..4219
                     /locus_tag="ACG8H8_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987612.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACG8H8_002597"
     CDS             4216..4554
                     /locus_tag="ACG8H8_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFP8987613.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DAZKAO010000165         4556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000165 DAZKAO010000000
VERSION     DAZKAO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.168"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8H8_002598"
     CDS             complement(<1..300)
                     /locus_tag="ACG8H8_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP8987614.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8H8_002599"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8H8_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8H8_002600"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8H8_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987615.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8H8_002601"
     CDS             1706..1942
                     /locus_tag="ACG8H8_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987616.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8H8_002602"
     CDS             1949..3901
                     /locus_tag="ACG8H8_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP8987617.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8H8_002603"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8H8_002603"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP8987618.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1556 a          802 c          754 g         1444 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttg
//
LOCUS       DAZKAO010000166         4476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000166 DAZKAO010000000
VERSION     DAZKAO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.169"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..881)
                     /locus_tag="ACG8H8_002604"
     CDS             complement(456..881)
                     /locus_tag="ACG8H8_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987619.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ACG8H8_002605"
     CDS             1255..2226
                     /locus_tag="ACG8H8_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8987620.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ACG8H8_002606"
     CDS             2258..3655
                     /locus_tag="ACG8H8_002606"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8987621.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="ACG8H8_002607"
     CDS             3814..4437
                     /locus_tag="ACG8H8_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987622.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1563 a          719 c          914 g         1280 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg tgaaatcagc gttttgacct gagtat
//
LOCUS       DAZKAO010000167         4455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000167 DAZKAO010000000
VERSION     DAZKAO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.170"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            325..1053
                     /locus_tag="ACG8H8_002608"
     CDS             325..1053
                     /locus_tag="ACG8H8_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8987623.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1198..3225
                     /locus_tag="ACG8H8_002609"
     CDS             1198..3225
                     /locus_tag="ACG8H8_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP8987624.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3333..4430
                     /locus_tag="ACG8H8_002610"
     CDS             3333..4430
                     /locus_tag="ACG8H8_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP8987625.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          918 g         1251 t
ORIGIN      
        1 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
       61 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
      121 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
      181 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
      241 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
      301 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
      361 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
      421 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
      481 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
      541 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
      601 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
      661 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
      721 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
      781 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
      841 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
      901 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
      961 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
     1021 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
     1081 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
     1141 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
     1201 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
     1261 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
     1321 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
     1381 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
     1441 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
     1501 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
     1561 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
     1621 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
     1681 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
     1741 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
     1801 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
     1861 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
     1921 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
     1981 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
     2041 caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat tatacctgat
     2101 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
     2161 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
     2221 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
     2281 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
     2341 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
     2401 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
     2461 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
     2521 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
     2581 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
     2641 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
     2701 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
     2761 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
     2821 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
     2881 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
     2941 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
     3001 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
     3061 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
     3121 acagctcatc gtggaatcag aataaatatt cctgtagaaa accaaagaat cggaaccttt
     3181 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
     3241 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
     3301 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
     3361 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
     3421 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
     3481 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
     3541 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
     3601 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
     3661 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
     3721 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
     3781 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
     3841 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
     3901 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
     3961 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
     4021 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
     4081 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
     4141 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
     4201 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
     4261 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
     4321 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
     4381 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
     4441 acaaatagta aattt
//
LOCUS       DAZKAO010000168         4440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000168 DAZKAO010000000
VERSION     DAZKAO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.171"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..521
                     /locus_tag="ACG8H8_002611"
     CDS             282..521
                     /locus_tag="ACG8H8_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987626.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="ACG8H8_002612"
     CDS             complement(883..1143)
                     /locus_tag="ACG8H8_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987627.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="ACG8H8_002613"
     CDS             complement(1328..1825)
                     /locus_tag="ACG8H8_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP8987628.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG8H8_002614"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG8H8_002614"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP8987629.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG8H8_002615"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG8H8_002615"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP8987630.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1295 a          938 c          692 g         1515 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
//
LOCUS       DAZKAO010000169         4390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000169 DAZKAO010000000
VERSION     DAZKAO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.172"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..659)
                     /locus_tag="ACG8H8_002616"
     CDS             complement(48..659)
                     /locus_tag="ACG8H8_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8987631.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(782..907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(989..1705)
                     /locus_tag="ACG8H8_002617"
     CDS             complement(989..1705)
                     /locus_tag="ACG8H8_002617"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP8987632.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="ACG8H8_002618"
     CDS             complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="ACG8H8_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP8987633.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2264..3034)
                     /locus_tag="ACG8H8_002619"
     CDS             complement(2264..3034)
                     /locus_tag="ACG8H8_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP8987634.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3031..3402)
                     /locus_tag="ACG8H8_002620"
     CDS             complement(3031..3402)
                     /locus_tag="ACG8H8_002620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987635.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="ACG8H8_002621"
     CDS             complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="ACG8H8_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP8987636.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1194 a          960 c          766 g         1470 t
ORIGIN      
        1 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
       61 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
      121 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
      181 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
      241 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
      301 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
      361 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
      421 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
      481 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
      541 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
      601 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
      661 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
      721 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
      781 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
      841 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
      901 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
      961 acatctaaca cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
     1021 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
     1081 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
     1141 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
     1201 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
     1261 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
     1321 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
     1381 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
     1441 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
     1501 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
     1561 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
     1621 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
     1681 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
     1741 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
     1801 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
     1861 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
     1921 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
     1981 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
     2041 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
     2101 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
     2161 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
     2221 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
     2281 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
     2341 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
     2401 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
     2461 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
     2521 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
     2581 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
     2641 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
     2701 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
     2761 cccataattc ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc ttgcttttga
     2821 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
     2881 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
     2941 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
     3001 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
     3061 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
     3121 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
     3181 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
     3241 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
     3301 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
     3361 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
     3421 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
     3481 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
     3541 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
     3601 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
     3661 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
     3721 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
     3781 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
     3841 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
     3901 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
     3961 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
     4021 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
     4081 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
     4141 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
     4201 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
     4261 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
     4321 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
     4381 caaagaaaaa
//
LOCUS       DAZKAO010000170         4363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000170 DAZKAO010000000
VERSION     DAZKAO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.173"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8H8_002622"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8H8_002622"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP8987637.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8H8_002623"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8H8_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8987638.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8H8_002624"
     CDS             2069..2821
                     /locus_tag="ACG8H8_002624"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP8987639.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3562..4095
                     /locus_tag="ACG8H8_002625"
     CDS             3562..4095
                     /locus_tag="ACG8H8_002625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP8987640.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1283 a          856 c          771 g         1453 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct tttgaacacc
     3181 gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa
     3241 aatagttcct catattttgc tattttttga gctaataccg agggtcagct gattgaacac
     3301 cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga
     3361 aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat
     3421 ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata
     3481 aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt
     3541 tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat
     3601 cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat
     3661 gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac
     3721 ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc
     3781 caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag
     3841 gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt
     3901 acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg
     3961 tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca
     4021 tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt
     4081 gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt
     4141 cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg
     4201 actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg
     4261 ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt
     4321 tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAZKAO010000171         4361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000171 DAZKAO010000000
VERSION     DAZKAO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.174"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..1479
                     /locus_tag="ACG8H8_002626"
     CDS             178..1479
                     /locus_tag="ACG8H8_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8987641.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1506..1766
                     /locus_tag="ACG8H8_002627"
     CDS             1506..1766
                     /locus_tag="ACG8H8_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP8987642.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2765..4000)
                     /locus_tag="ACG8H8_002628"
     CDS             complement(2765..4000)
                     /locus_tag="ACG8H8_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8987643.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1272 a          688 c          891 g         1510 t
ORIGIN      
        1 aatttttttg ttgctcctat gatttagaaa acttattttc aggattctta aaaataaatt
       61 tgattattta agagaatata ttgaaataaa atttcttaat tatataatta gttatgaaca
      121 ttctttgcgt tgtcatgcaa tgaatggttc atattttatt gaaaaggagt ggttttagtg
      181 gaaaagctga tgaaatgggt cgaagagaaa cttgtcccac caatggcaaa aattggtaca
      241 cagcgtcatt tattagcaat tcgtaatggg gttgtttcaa cattgtcatt gattttgatt
      301 gggacatttt ttatggtctt tatcaattta ccgtttcctg gatggaatga atttattgcc
      361 ccttattcgg caacgattgt tttacccttt aggatgacga tgggattgat ggctatttat
      421 gcaacttttg ttatgggttc ggatctagct aaatcatatg gattagatag tgtgacaggt
      481 ggtatattat ctttaggaac gttctttatg ttacaagttc ctgtaaacgt tttaactcct
      541 gaagaggcac ctcttggctg ggtcttacct atgagttctt tgggagcatc tgggatgttt
      601 gcggggatct tatctatgat ttttgcagtg gaagtatatc gtttctttaa aaatcgaaat
      661 attactatca aaatgccaga acaagtgcca cctgctgttg cccgtagttt tgaggcattg
      721 attccaggtg cagtcatttt aacaattact tggctgattc gaagcgtcgc gggctttgat
      781 gtaaacgctg ccttattatc gttattcgag cctttaacca atattttagg gaataatttg
      841 cttggtgttt tactaccaat gtttttgatc catttgttgt ggtcatttgg tatccatggc
      901 atgagtattg taggggctgt tgttcgtcca atgtggctga tcatgttgga tgagaatgca
      961 aaggcattgg cagatggtac agctgccacc caattaccct atatcgctcc tgaacagttt
     1021 tatcaatgga cggtaacaat tggtggtgca ggagcaacga tcgtggtatc tgttttgttt
     1081 atattcttct gtaaatcaaa attcttgaaa gaggttggtc gtttttctat tattcctggt
     1141 atcttcaata tcaatgagcc cataattttt ggagctccta tgatgttgaa tccttacatg
     1201 ttcatcccgt tcaacttagt tcctttagtt ttgactatta tcagttattt tgcagtcaaa
     1261 ttagaaatgg tcaatagatt tactgttttg ccaacatgga cattaccagc tccaatcggc
     1321 ggttatcttt cagcaggaaa tgactggcgt gttgttatct tgatcatgat caacacattg
     1381 atagcattta ttatctatta tccatttgta aaagcttatg ataaaaagat gctgtcagat
     1441 gaacaaagtc gtggagaaaa cgaagtccag actgtctaag tttggttttt agaaggtggg
     1501 aaacaatgta ttggctgatt ttattttttg tttttatctt tttattaacg gctagtcacc
     1561 ttatattgaa tatgctagct acatatcata ttcaaatcaa tcgatggatt tgggcactgg
     1621 catcattttt gatcgtgatt cttccaaaga tcattgtacc gcatatgaat gttttgtttt
     1681 catgggggac atatgtactt tgtggaattt ttgctatcaa ttttatgatc gagcagcata
     1741 gatggtttgt cacatcgaag ctttaaatgt tctaaaagtg gttgtaacag aagtggttag
     1801 cttcaagaaa taagaagaaa tttacgaaaa ttgcttctta aatttttgtg aaatgttggc
     1861 ttatttttcg tttggttgaa cttattaccg cagatgcaag cttttgacca ccgtttattc
     1921 ggaatatggg actgtgacaa ggcttttgtc acagcccttt tttacataaa gattattatt
     1981 gttttaactg ttttggcaga aagaaggagg aaaaaatgga aattttatca gcaatggatt
     2041 tagaattagt agatctacta ccaaagtgat caagtcaaat atagatttgg gagcttacaa
     2101 ccatatagta ggggctgcag tatggtgtat gaatggtaat atgtatgatg tgagtgttta
     2161 ttcaaatcat gaaacgtgta tagaaataat tgtgctggga atagcaatca gtcatggtga
     2221 acgtgacttt tagtgtatcg ttgctgacga catagaacat tctgacatga tctactcaat
     2281 atgtggggat tgctggcagt ttatatagga gtatgcatcg gattgcgaga tcattgtctc
     2341 aacagaagag ggaggttgat tatcaaagat aaagtggtaa atcctacatt tgatttagta
     2401 attgttgagg ggactagcgg cacaaatctc tacaaagcga tgcttctaga tctatatgac
     2461 acattttaag aaaatgtatt tgcctaatta ttatggtctt tcttttgaag aaacgttgag
     2521 atgatccaac cagagaaagg tgtcaaagaa tttgactaag aataaatggc agacgctacc
     2581 tatgctcatt tggatagcgt cagatctttt attttaagga tgattttgtg ttttttagac
     2641 accgtaattt aagtaactct aacttttcaa tccactattt actttttttg tattttcact
     2701 gtctgggtct cgaatagcga aattactgat gagtctctat taaaaaagct ttttctactt
     2761 gaaactatat tgggtggttg ctcaggaagg tttcgatttc ttgaattgct tgttgtggac
     2821 catttattgg aaaaacggct tgcagcgtgc gtgcctgctg acgaatcttg ggtgaagaaa
     2881 cttcgcgcag agcaatctgc agttttcttt cagtcactgg ttttgtgagg gcgattgcgt
     2941 ttccgtattt tacacaatag tcaatattac agctttgctc aaattgcatc cccacaccga
     3001 taaacggttt gccggattca caggcagttt ggacggtccc ttccccgccg tggataaaag
     3061 aaaaatcaat gaattcatgc aaaagatgac tagacagata ttctgttatt aaaagatctt
     3121 ctgagtcaga ttgaatgtca tcaagcagat agtcaatagg tgcaataacc gctagatccg
     3181 gttgtttttg taagaagaac aaaattttgc gtaacatacg cggattgcct gaactaccca
     3241 ttgcgaaata gataatacgt tttctttctt gttgctttcg ctgtataaaa gttagcactt
     3301 cttcaggcaa ggaagtactg agcttagcaa aaattggacc aacgattcga taattttccg
     3361 gcagctgttc ttcttccaaa aatagtttag gcgttgtgat gagatttaga tcagcatcca
     3421 tcatttgtaa acttgttttt ggtaaaggaa gcatttcttt ttgtgcagcg tgtttgaagg
     3481 cattggggat ccatcgggta tttactatac gcttttttgt ccagtgccaa aggggattgc
     3541 ttaaagggcc gaagaaacgt aactcgtggg gcatcagcgc atgctctgat tgaaaaaaag
     3601 gttcactcaa agcatagggt ttaacatata ctaaaggaat ctttttattg cgggccgata
     3661 aaaaaattgt cacattcgaa cctgtcacaa tcagtcgagg ctcaaactta gcaatcagac
     3721 tctgttcact agtgatacga gaggcaacca tttctttggt aaaagggttt ttaagtgtac
     3781 gcaactggtc aaattttatg atttgttctt cttgtttttt cgtaaggatc ggcgctagta
     3841 aatgatactc aaagccactt tctgtgatca aaggagcaaa agttttagaa aagccaaaga
     3901 atacacagta ataacggtct ttcatagctt tggcaacctc aatcattcgt gttgtctcag
     3961 ctaaattgaa tactgcagga gcaaaaagaa ttgtcttcat gaaatcctcc ttattggaat
     4021 gagtctatcc cttcgtttct tactagtata ctaaaaaaca cagcgttcga gtagcctatg
     4081 tatattttga atattaaact gtctctcgct gttttcacaa cttctaaatt taaattttgt
     4141 aatcaagttg aaactccaat tttcaaaaaa acacgcaaca attttttgat cgatttatat
     4201 gttgtgccag ataaatacga gaagttgtgt cattaaaaat aaacatgaga acataaaaaa
     4261 ataaaataat tatattaatt gataggtgtg ctgtcgtcat tatacacacg taagaaagaa
     4321 atagttatgt ttaaaaatat tcttagactt tctcgccatg a
//
LOCUS       DAZKAO010000172         4182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000172 DAZKAO010000000
VERSION     DAZKAO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.175"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1779
                     /locus_tag="ACG8H8_002629"
     CDS             16..1779
                     /locus_tag="ACG8H8_002629"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP8987644.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1863..2321
                     /locus_tag="ACG8H8_002630"
     CDS             1863..2321
                     /locus_tag="ACG8H8_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP8987645.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2430..3113
                     /locus_tag="ACG8H8_002631"
     CDS             2430..3113
                     /locus_tag="ACG8H8_002631"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP8987646.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3260..3874
                     /locus_tag="ACG8H8_002632"
     CDS             3260..3874
                     /locus_tag="ACG8H8_002632"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP8987647.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3890..4096
                     /locus_tag="ACG8H8_002633"
     CDS             3890..4096
                     /locus_tag="ACG8H8_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987648.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1493 a          681 c          876 g         1132 t
ORIGIN      
        1 aggagagaat gtattatgag tacagtatta catgtattgg atgcaatcaa tagccccgca
       61 gatgtcaaaa aattatctgt tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa
      121 atcttgcaaa aggacagcga tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa
      181 ttaacgattg cgtttcatta tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt
      241 tcccatcagt cttatggtca taagctttta actggtcgaa aaaatggttt tgaaaaagga
      301 cattaccgtg aagtgagcgg atattccaac caacatgaaa gtaaacatga ctattttaca
      361 gtggggcaca catctacatc tatcagctta gcaacaggtt tagctaaagc gagagatctg
      421 aagaaagaat caggaaatgt ggtagcattt atcggtgacg gctccctaag tggtggctta
      481 gcttttgaag gattgaacaa tggtggtgtt ttacaatcca atctaatcgt attagtaaac
      541 gataatgaga tgtctattga tgagaatcat ggcagtttat ataaaaactt agctgagctt
      601 cgtcaaacca acggtaaaag cagcaataac ctatttaaag caatgggatt tgattatcgt
      661 tacgtggata atggaaacga tctggaaacg gtgatccgtt tatttgaaga agtaaaagat
      721 attgatcatc cgattgtact tcacgtccat acggaaaaag gtcgcggtta tcagccggca
      781 atcgataata aaatgaaata tcattggcat gtaccatttg atctgtccac cggggaggca
      841 aagcaagtaa cttctggaga aacatttaat atgatcatca tggattattt ggataagaaa
      901 atgcaaacaa gtacagctcc tattgccgca gtcaatgcag cgataccagg cgcctttgga
      961 ctgaaaacat tccaagaaaa acatccagat cattattttg acgtggggat cgctgaacag
     1021 cactctatca ctttcgcttc tgctttggct tctcagggag tacgtccagt cgtcttccaa
     1081 tcaagtactt tcttgcaacg ggcatatgat caattatcac atgatttagc gatcaacgag
     1141 aatccagtga ttattttagt cggtggcgga acgatctcag ctagtgaccg aacacaccaa
     1201 ggaacatttg acattcctta cttatcaagt atcccgaaca tcaaatatct tgctccagca
     1261 acaaaagagg atatgatctc tatgctagat tgggcgttcg aacaaacagc gcagcctgta
     1321 gcaatccgaa ttcctacaca tggtgtagaa catggtacag ctatcgcaaa tgattatagt
     1381 attccagcct atcaaacagt aaataatgga ggaaaagtag caattttagc tttaggcggt
     1441 tttcttgaat taggaaaaga aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg
     1501 ttgatcaatc caggaacgat cacagaaaat gacgtagaaa cattagataa attaaaagaa
     1561 acacacgaat tagtcatcac attagaagat ggtagtttgt caggaggatt tggcgaaaat
     1621 atcagtcgtt attatggtga ttcttcaatg aacgtattaa attttggtgc agagaaagaa
     1681 ttcacggata gcgttcctgt agggcagctt tatgaacgtt atcatttaac accaaaacaa
     1741 atcgttgaag atagtctgcg tgtcttgaat attcaataat tcattatgct caccatccag
     1801 ataattgttt atactagtat ctggatggtt ttttcattat aaaaagcaaa ggggtggatg
     1861 ttatgtatca gcaagtagaa gaatatgtca aaaagttcca agaaaaatat cctagttatc
     1921 aatcagaaaa attaggtttc ttttcatttg gggatacaga aaaaatggca gacgagttga
     1981 gcgaactagt tgtaaaaaag ataaagactg gtacatcaag tggttacgat ctgtataaac
     2041 aagagaatga gcctttacca aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg
     2101 aaccagtttg tatcatcaaa aatataaatg tgaccatctt gccttttagt gaagtcacga
     2161 aagagcatgc ttggaaagaa ggagaagggg atcggacatt agcttattgg cggaaagtcc
     2221 atgctgagtt ttttaaacat gcttataaag aagcgttgaa catagaattt acagaagaga
     2281 agctggtcgt atacgaagaa tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa
     2341 gaaatagctc attattgtct cctgaataga aaagggttac atttttatat gaagctgtat
     2401 ttttatataa cattactgga ggagatattg tggatacact ggaacaaatc aaaggcggag
     2461 tcgtcgtttc ttgtcaagct ttggaaaacg agccgctaca cagttctttt attatgggga
     2521 gaatggcttt ggctgcaaaa gaaggcggag cagttggtat tcgagcaaat acgacagaag
     2581 atatcaatga aatcaagcga atcgttgatt taccggtaat agggattatc aaaagagatt
     2641 acgatgggtc agaagtgttt attactgcga cagaaaaaga agtggaagaa cttttggaga
     2701 cgcaagcaga aatcattgca ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa
     2761 ctgctgaact tgtccaaatg attcacgaac acggtcggtt agctatggca gatatttcga
     2821 cttttgagga agcgcttcaa gcgcaagaag atggttttga tcttttgtct acgactttag
     2881 caggctatac agaatattca cgaaagacgc agacacctga ttttgattta ttgaatcaaa
     2941 tcatagaaca agtcgatatc ccagtaatta tggaaggaca tactgactct cctgaacaag
     3001 tagagaaagc atatgaatta ggtgcttttt cagtcgtagt tggatctatt atcacccgtc
     3061 cgcaattgat cactaaaaga tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac
     3121 gcacggcaca gattcttgac aaaaaagaaa aattgataga tagtttaaag cagattctaa
     3181 gaagatgaaa aaagcattac ttttcatttt gtcttcaaga tctgcttttt aacgaatttt
     3241 atagaaagaa ggcgaaacca tgtttgaacg gagtccttca gaattgacag aacgtgaaaa
     3301 ttacaagttg ctgattggtt cagtcatccc tagaccagta gcagtagtaa cgactaaatc
     3361 agcaaaagat atagtgaata tcgcaccctt cagttacttc aatatcgtca gttcgaatcg
     3421 acccatctta tccttggcga tccagagaaa acaagatgca gtaaaagaca cagcaaagaa
     3481 tattcttgag acaagagaag ctgttatcca tatccttgat gaggacaacg tagaagaagt
     3541 aaataaaaca gcagccaatc tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac
     3601 tttaactgat tcagtaatca tttcagtacc agcattgaca gaagcaaaag ttcgttttga
     3661 aacatcctta tatgaacatg tcgagatacg gaataaaaat aaagtaacag ctgatttatt
     3721 tttgctagaa gtacagaaat atcatttaaa tgaaacagtc tatgatgata agacaggaag
     3781 gatcgatcca agaaaattga atgctgtaag ccgtttggca ggaaatgatt atgcagcaat
     3841 aggagaaatt ttcacgattg cacgtcccga ataaaaagga gcactagtta tgttaaaaat
     3901 aagctataaa ccatcgacag attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga
     3961 ttttctgcaa gggcagtact tagaagttcc gccattgcaa gaccattttg tcgttactac
     4021 tgttacttta gatggtaagg aaatagaaat gccagatcaa acgattagcg gattgtttaa
     4081 ttactttaac aaataaatat ttattccgaa aagtgaaaaa acatataaaa aaacacataa
     4141 aactaaaaaa ttatcaaaga atcctggtgc aactgaaaga at
//
LOCUS       DAZKAO010000173         4107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000173 DAZKAO010000000
VERSION     DAZKAO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.176"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(217..1722)
                     /locus_tag="ACG8H8_002634"
     CDS             complement(217..1722)
                     /locus_tag="ACG8H8_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8987649.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1719..2405)
                     /locus_tag="ACG8H8_002635"
     CDS             complement(1719..2405)
                     /locus_tag="ACG8H8_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8987650.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2598..4019)
                     /gene="gndA"
                     /locus_tag="ACG8H8_002636"
     CDS             complement(2598..4019)
                     /gene="gndA"
                     /locus_tag="ACG8H8_002636"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP8987651.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1132 a          864 c          695 g         1416 t
ORIGIN      
        1 aattccctat ttttatttat tatttttaaa cacgaatatt acagcatttt attgtttttt
       61 cgttcatcta aaagatactt acatttaatt aacggatgtt attctattta attgttcgct
      121 agttttttta taatgacatc cctgaatttc tcacttgctt gtattttgta ccttattctt
      181 tgttggatat ataaaaaaac aagtaaagca catgctttac ttgtcttctg cctttgcgat
      241 aggaataaag attctaaaaa ttgtcccttg ccctactaca ctttctgcta agatacgtcc
      301 tttatagttt tcaaccagtt gtttagcgat agacaacccc agaccatttc cacccttcgt
      361 tctagctctt gctttatcca ctcgataaaa acgattgaag atcttattaa ggtcttcttt
      421 gggtattcct tcgccaaagt cctgaatcgc gatctcaaat tcattcagag tggaagaaat
      481 agagatatgg acttcttttc gtgtagttga atactttact gcgttatcca atatgataat
      541 gattaattgt tcaaagtgat tacgataaat ttgcagggtc acttcttttg tcaaatcatc
      601 atctaaagtg atcacatagt ctggatataa tatcttgaaa ttattataaa cttgataggt
      661 gacttctttt cctttagttg tttcattcgc ataatgaaca tctacttgtt ctgctcttga
      721 taagtctagc atctcttgaa ctaagctttt catacggctg atttcttgca tgctggcgct
      781 taatgattct tctaatatct caggatcatc tttcccccaa cgatttagca gactaagatg
      841 cccttcaata attgcaacag gtgtccttaa ctcatgagat acgtcttcca cgaattgctc
      901 ttgttgttca atatacaaac gcatacggtc aagcatctca ttgaatattt cagctaaatc
      961 tgccaattca tcatttgtgt cgatttcagg catgtggata tctgattgcg gatctttacg
     1021 tatagtatcc attgtatctc tgagtacctt gagtggtttt aagaaataag aagataaaaa
     1081 gaaacctaaa atactactta agatcaaaga aacaacttct aaaacaataa gagtcaataa
     1141 tagcttattt cgaatatcat agaaagaaga taattcatag aagctttgcg catagccgat
     1201 cttttctctt gtacctttag aatatatagg ctggatagat aaaaagccag tttttccttc
     1261 tagtgtaacg atcgttggaa ctttacgtgt agtctgaatc aggtttcgac tgttttcatg
     1321 ggttttaaaa atcaagttct cgtccaaatc ataaacataa agatccattg atggctgccc
     1381 tagctcagct ataaaggaat cgatcttcat taagtttcct tcgaccagtg aagtatgatc
     1441 tacgacctca tctccgtcat ttgtcgcttt cgtcagatta cgatacgtat ttactaatgt
     1501 cagttcttca tccgagttag ccaaacgaga agctacttct gaaatcgtac gctctacatt
     1561 gtttctttct tttgccacaa tcagattgat agatgattta taggtaatga cagcgaaaat
     1621 tgtaaacaca acgaatatga agaaagaact cgcaaaggcc cacttgattg tcaaggaggg
     1681 ccctttcagt tctctttttt ccgatttttt cggctttatc acgagcgcat cacataacct
     1741 gttccgcgta cagtttggat atagctttcc tccccgggaa cgtcaatttt gttgcgaaga
     1801 taacgaatat atacgtccac tacgtttgtt tcaacttctg tttcgtatcc ccacacttta
     1861 tttagtaaaa cgtcacgtgc taataccacg ttcacgtttt ccattaaagt aagcagcaac
     1921 tcgtattctc tttttgttag ttcaattact tcatttccgc gacgtacgac acggttttct
     1981 ttttcgatag tcaaatcgcg ataagtaata gtagtttgtt tagccacatt tttatcgcct
     2041 tcaatatcga ttcgacgcaa caatgcacga agacgtgcta ataattcttc gatagcaaat
     2101 ggtttgacga tgtagtcatc tgctccgtgg tcaagtccag atacacgatc gatcacagaa
     2161 tctcttgcag tcatcataat gattggtgta tttttgactt ggcgtacacg tctgcagact
     2221 tctaaaccgt tcaattctgg gagcatcaga tccaacaata tggcatccca ctcattattt
     2281 aatgccgcat ctaaaccagt tcggccgttg tagtggactt ctgcgttata cccttcatgc
     2341 ttcaactcta gctcgacaaa tcgtgccaag ttcttttcat cttcaatgat tagaatattg
     2401 ctcatttatt attttatcct ttctattcta ctggcttttt aaaaactcta tcattatacc
     2461 gataatatgc ttaaaaatct atagtcattt tatcatggat agcaatttta aaaaagagct
     2521 gaagtctact cataacaatc gacttcagct ctttcgtcct ctttttattt tctcatttcc
     2581 taacagccga aagactatta ttcttcatgg taccatgagt aatggaagat tccttctttg
     2641 tcgacacgct cataggtatg tgcgccaaag taatcgcgtt gggcttggat tatatttgca
     2701 ggcagacgtt ctgctctgta tgaatcgaag taagcgatgg ctgatgagaa agtcggtaca
     2761 ggtacgccag cttggacagc caaggcaacg acgtctctga ctgattgctg atattttttc
     2821 gtgatttcaa cgaagtaatc atctagcaat aaattttcga tctcaggatt tttgtcatag
     2881 gcatccgtaa ttttttgcaa gaatcgagct cggatgatac atccagcgcg ccagattttt
     2941 gcgatttcac caaatggcaa gtcccaatca aattcttttg aagcgacacg taattgagca
     3001 aatccttggg cataactcat gattttactg aaatacaacg cttctcggat tttttcgacc
     3061 aattcttttt tgtctcccgt gaactcaaaa tcgttcgtac gagacaaaat cttgcttgct
     3121 tgtacgcgtt cttctttata agcagaaata tagcgggcaa atacagattc agtgatcaat
     3181 ggaagtggca cacctaagtc aagcgcgctt tggcttgtcc atttacctgt tcctttattc
     3241 ccagctgcat caaggatcac atctactact ggtttacctg ttccttcatc gtctttacgt
     3301 gtcagaatgt cagcagtgat ttctatcaaa tagctgtcta attcgccttg attccattct
     3361 ttaaagatat cagccatttc ttctactgaa aggtttaaaa tattcttcat taagtcataa
     3421 gattcagcaa tcagttgcat atctccatac tcaataccgt tgtgtaccat tttcacataa
     3481 tgtccggctc catttgggcc aatatatgtg acacatggtg ctccgtcttc tgcttttgca
     3541 gaaatttgct ctaagattgg tgcaactagc gcataggctt ctttttgtcc accaggcatg
     3601 attgaaggtc cttttaaagc tccttcttca ccaccggaaa cgcctgtacc gataaagttg
     3661 atgccagagt tggctaactc ttcattacga cgcatcgtat ctttaaagaa tgtatttcct
     3721 ccgtcaatca aaatatcgcc tttatcaagg tgaggaagta actcttggat tgttgcatct
     3781 gttgccggtc ctgctttgac cattagcaag atccgacgtg gtttctcgat agattctaca
     3841 aattcttcga ttgtatatgt tgctttgaaa tttttatctg ggtgttcttc tactacagcg
     3901 gttgttttag cccctgtacg attgaataaa gccactgaat agccgcggct ttctatgttc
     3961 aaggctagat ttttacccat aacggccata ccgacaacgc cgatttgttg ttttgacata
     4021 tgaactcctc ctacatacgg gatttctccc taatctctta cattatagct taacacggct
     4081 acattgcaaa ctaaagaaca acatatt
//
LOCUS       DAZKAO010000174         4033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000174 DAZKAO010000000
VERSION     DAZKAO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.177"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2727)
                     /gene="brxC"
                     /locus_tag="ACG8H8_002637"
     CDS             complement(<1..2727)
                     /gene="brxC"
                     /locus_tag="ACG8H8_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP8987652.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTM"
     gene            complement(2752..3327)
                     /locus_tag="ACG8H8_002638"
     CDS             complement(2752..3327)
                     /locus_tag="ACG8H8_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFP8987653.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3327..3926)
                     /locus_tag="ACG8H8_002639"
     CDS             complement(3327..3926)
                     /locus_tag="ACG8H8_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFP8987654.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1204 a          776 c          546 g         1507 t
ORIGIN      
        1 cattgtatgt tcattgttat tttcatcaac aatattacga ctttgaaaca gttctctacc
       61 gatttcgtca accaattttt ttgcttttgg cgaaatcacc gcgcttggtt caatttttat
      121 cttttcactt tctcgtcgtt tttgaataat cattacaata ctatttggat catccatccg
      181 tgaaatttgt acattgttat aaagtaaatc aattttacca gacatgaaaa gtttagcgat
      241 gcaccattcc acatcaagtt ctgtgaatcc atacggttgt tttgtttcaa aacgatcacg
      301 aattgttttc atcgtaatcg ttaggtgatt tctcttttga tcattgataa aattgaaaat
      361 ttcctgaact gcttgctcat ttgatggatc ttcaataatt tcatttttgc tttctttcac
      421 taaatataag atatcttctt tttctttagg cgcatcaatt tggtttaatt tatggtatac
      481 ttcttcagcc attttctgaa gtgctgattg gaattttgct ttaaagtcgg agcctgtcag
      541 ttgaatttct gcaccgttat agaaaaattt tgcagatttc aacagttcat ttaattgatc
      601 gttgatactt gcacttagtt cctgtaattc accagatttt tccgaacgaa tcgcgtcaaa
      661 gttctttact acactcgaat aagtcgttgt taaataaaga ttcattctag catacattgc
      721 tatatcatct ttaaattctt tatctgatgg caattctacg aataataagt tacgggaact
      781 cttaagcgca agttctgcct cttgacctgc taaatcagag tatggtgaaa taaaattaac
      841 tgtcatttca gcatttccag cttgcttgta tggacgttga tctaacactt gtgcataagt
      901 aaacgtatac cggtttttaa atttatttcc acctgacatg cctaattttt cataggcaaa
      961 actatttcgg tctagaacgc cgtcaaaaat tcgtttagat agttctacga tcaaatcact
     1021 atctgtaata ttttgatttt taatctcacg agtaatttcc tgttcttcat cagtttggaa
     1081 aatataacta tctaagttct tccgcacaaa gttttcctta attaaaaggt ccaatgcttc
     1141 ttctactcgt tttttagttt ccaggcggtc ttcgttgata tttcttatca tcaagctcgt
     1201 aatattttca acatttggtt tgatggcttg atcaacatat ttaatcatga ataaaattcg
     1261 taagacattt acgttaaagt tatctatatc agttggtctt tgaatatgag aattatttgt
     1321 agcttgtgta atcactcggc taattgctgt atcaatccaa cgttctaacg agtaataaaa
     1381 ctgatctaaa gaaaccaaac tggttgtatc attttcctta tttcgttcag cggcttcttt
     1441 aaacattgct agtaatgaac gttcaccaga cgataagttt gatccttgat agctgtgatt
     1501 acgaatctcg gttagaattt gccccaataa attgaattga tagggaataa atggataaac
     1561 ttctgcgtaa ttttcagcat cttggaagag ctttaattca ggactatcaa caaagagtaa
     1621 tttatttttc aattctgttt gaacagtttc aaattgtaag gctaaagaat cattggcaaa
     1681 ctcttttttc ttcaaaatac gttctttaat tacttcatcg gcatttgctg aagataaact
     1741 cacagttgtg gcaaaacgat ccttgatctt agaaaaatca ttatcagaat tgttatcaaa
     1801 gtttgttacg tgatcaatcg cttcctgact tgtcactaat acccaagcct taccttcaca
     1861 aattcgaccc aaatcttcca caacggtttg taactgcaaa gtcaactcac gattaccgcc
     1921 gatatattgt ccgacctcat cgactagaaa ggcaatatga tggtctgctt ctttggaatc
     1981 aagatagctt ttaactagat ttgcaaagtc atcaatgctt attaaataat ttcctgatgc
     2041 tttttctaaa aaattatcaa tagcggcttc actcataaaa tcgattttaa gtgccgcttc
     2101 tttgatatca tcttggatcc aatcaaactc atggcgctct tcttgccatt ttgccccatt
     2161 aatctcttca aaagcagctt tgaatgcatc aaacttttct tctttaatta accgacgttc
     2221 aaaatctgct agatgtggaa tcgtgcttaa tccttgcatt tcgttaaata cctttaaaaa
     2281 aacattcacg attccgtttt tgtctttttt accacctgca tcactttttg aatcaatatt
     2341 aaacagaata acatctgtat ttatttgtga gcaccgttcc atcgttgcca aagtcagtgg
     2401 atctttgatt ttcccatctt ctttgaagta atcaatggct ttcttgtcat ttatttctcg
     2461 atttgcgagc aagtaagaaa taatttttaa gaaatgagat ttacctgaac caaagaaccc
     2521 ctgaatccaa acacccattt tatctgttgg tgtattgata ctccgttggt aggcatcaaa
     2581 aattgtttta aagtgttttt gtagttcttt cgtaaccacg tattcttcta attcttgttg
     2641 aacattttca tcctttgctt gaccgacctt aataactcct tgaatatttc ggtcaattgg
     2701 ttttgcaaaa atatctttaa ttagcattta atctcctacc ttccatccca actaaattct
     2761 aaatgctcga taataattat catctttatt ggtattaaga attgtcaaac ttaattcatc
     2821 atattcccct ggatacatca tcacaactgg tacgcgatca aatacctgat gcattttatt
     2881 taaaatagag tgcgctctaa ttaatggata taattcgcca attcctgtca ggaacacaac
     2941 tgatttttct ggtgtatggg catccacata ttcaaaaaat acattttctt cctcaggtaa
     3001 atccattttc aataaacgac caatgctttc cactaatttt tgtaaaccac gtcgttcttc
     3061 catcttaatc gctttttcag tgaaaccgcg tttttctaga tactctaaca taatcacata
     3121 gagattataa actttcactt cataattatc accaacagat gttccagata tatcattaat
     3181 ataatctctt acaattaact cgtctttcgg atcgtagtca aagatataat agcctacttc
     3241 attacctaaa ccacgattca ataaaaaatc ttctttttga attacttcat tcaacttttc
     3301 caaatgctct tgaatcgata atgacattat aaaattcctc ccagtgctag tacaatatct
     3361 ttacgattct tgtcaactaa ccattgaatc aatcgtttat ctaataatat cggagtcatt
     3421 cgtccatatt ctttttcccc tctgactaat cctgtttcaa ttagattcgt ttgataagcg
     3481 gcaataagcc gtttaatcgt ataatctttc cattttgaaa tatcttctga acttctccgc
     3541 ttctctgtaa aaaaagaacg gtaatcaact ggtgttaatt gcaaatttcc tttacgtaga
     3601 ttttcagcat atacctcagt cataaattca ttaaaaagat catcaatcat aaggatggaa
     3661 atcaaattca ctaacttttg attatctata tttaactgat tgaaaagttg caatccagct
     3721 tcatccagtt gactaactct ccttttcacg ttctgataga tttctttttg acggttactg
     3781 ctcactgcaa aaaaaatatt ttcatgttca tttttctctt taatttctat atctgtatat
     3841 ccttctagtc gtaattgaat atataacttg aactctgaaa accaaaagcg tttagaaact
     3901 agaccagcag aatatttttt attcaataga ttgcctccta atcatctata attcaataag
     3961 taaacaagtg ttttttgctc atcgttagca ttcatttttt agtatctact atacaataca
     4021 tatttattat agt
//
LOCUS       DAZKAO010000175         4023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000175 DAZKAO010000000
VERSION     DAZKAO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.178"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1436
                     /locus_tag="ACG8H8_002640"
     CDS             87..1436
                     /locus_tag="ACG8H8_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8987655.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1564..2154)
                     /locus_tag="ACG8H8_002641"
     CDS             complement(1564..2154)
                     /locus_tag="ACG8H8_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP8987656.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2301..3653
                     /gene="brnQ"
                     /locus_tag="ACG8H8_002642"
     CDS             2301..3653
                     /gene="brnQ"
                     /locus_tag="ACG8H8_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP8987657.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1152 a          741 c          785 g         1345 t
ORIGIN      
        1 gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg aagtaattaa
       61 acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag cgtggactga
      121 aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg ttgtttttag
      181 gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat cttattactg
      241 gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca aacaccaata
      301 accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct ttcatcactg
      361 gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca gccatcggaa
      421 tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg attgcattag
      481 gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta gagttttggt
      541 ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct tatatgatcc
      601 tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg tctcatggcg
      661 gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta acctttgctt
      721 ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct gaaaaagtgt
      781 tgccggaagc tatcaataat attcctatca ggatcatctt attttactta ggatctcttt
      841 ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt ccatttgtag
      901 aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta gtcgtattaa
      961 gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg cttcgttctt
     1021 tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat catgtgccag
     1081 ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg aattacgtga
     1141 tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc ttgtttatct
     1201 ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc aaaaaagctc
     1261 attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt tacatggcct
     1321 ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta ctaacacctg
     1381 tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag aagtaagaaa
     1441 gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga aaattgcttc
     1501 ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt cccagcctct
     1561 tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat aggaaaaaat
     1621 ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa aaaagacacc
     1681 ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct ttgacaaaac
     1741 tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca agataccaaa
     1801 agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa aagcttcagg
     1861 gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga gcaaaaagat
     1921 attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac gatgatggtt
     1981 cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg tatttcggat
     2041 gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata caagaactct
     2101 caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg tcacgatgat
     2161 tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat aaccatcgtt
     2221 ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt acttttaaaa
     2281 aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat ctttttatag
     2341 gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc gttcatttgg
     2401 gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt accggtatcg
     2461 gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt gtatatgaat
     2521 tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt tacttagtta
     2581 tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt ggtttagcac
     2641 catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt gtattttttg
     2701 tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt ggaaagttcc
     2761 tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt ttgaatccac
     2821 taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct tttttcacag
     2881 ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt gggatcatca
     2941 ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct aaagacacga
     3001 tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg ctcgcttatt
     3061 taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc acgctggctc
     3121 agattgctga tcattattta ggtacttatg gcagtatctt attagcattt atcgttatat
     3181 tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca ttcacagatt
     3241 tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt ccttgtttag
     3301 cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg atgttcatct
     3361 atccattagc aatgacactg attttactag tgttagttgg tcctttgttt aagcagcgta
     3421 cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt gatgggttga
     3481 acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta tatgttgcgg
     3541 aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca gttatcggtt
     3601 ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat tgataaaaga
     3661 ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa atggcttctc
     3721 acatttttgc ataaatttga cttaataccg agaaactgac ttttgaacac cgtttattga
     3781 gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga aaatggcttc
     3841 tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac accgtttatt
     3901 gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag ctccatatac
     3961 tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca tttcttttat
     4021 aat
//
LOCUS       DAZKAO010000176         3997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000176 DAZKAO010000000
VERSION     DAZKAO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.179"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACG8H8_002643"
     CDS             complement(243..392)
                     /locus_tag="ACG8H8_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987658.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACG8H8_002644"
     CDS             854..1066
                     /locus_tag="ACG8H8_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987659.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ACG8H8_002645"
     CDS             complement(1344..3143)
                     /locus_tag="ACG8H8_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP8987660.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESMKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ACG8H8_002646"
     CDS             complement(3267..3863)
                     /locus_tag="ACG8H8_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8987661.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1434 a          705 c          687 g         1171 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc atactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatcc cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaa
//
LOCUS       DAZKAO010000177         3896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000177 DAZKAO010000000
VERSION     DAZKAO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.180"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG8H8_002647"
     CDS             289..1104
                     /locus_tag="ACG8H8_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987662.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG8H8_002648"
     CDS             1230..1487
                     /locus_tag="ACG8H8_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987663.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG8H8_002649"
     CDS             complement(1739..2311)
                     /locus_tag="ACG8H8_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987664.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8H8_002650"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8H8_002650"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP8987665.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3333..>3896)
                     /locus_tag="ACG8H8_002651"
     CDS             complement(3333..>3896)
                     /locus_tag="ACG8H8_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP8987666.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1294 a          715 c          582 g         1305 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa
     3121 catggcatac tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat
     3181 ttcctttatt tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat
     3241 ctctatacta caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa
     3301 tctagaacgt aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt
     3361 tttgcagcct ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca
     3421 accgctagta aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca
     3481 ctataagcaa gagccattcc taataaatag ccaattaacc ctcctgaaag agttaaaatc
     3541 aaaccttcta aaaggaactg cagcataatc gattttcgag tagctcctag ggctcttctt
     3601 ataccgatct cttttgttcg ttcagataca gatatataca tcatgttcat tacacctacc
     3661 cctgcgataa ataaagatat acctgctaca gctgttataa aataagtgat cgtactgagg
     3721 atttgaccaa ttcctttcgt taacatagcc gtatctaaca cttcatattc ccccaaatgt
     3781 ttcaaactac ctttttcttt caattggttt atcactttag aggtcacctt gtcaggttca
     3841 actccttcct ctaaagttaa cgttagagac gatgtatcct tttcagactt aaaata
//
LOCUS       DAZKAO010000178         3843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000178 DAZKAO010000000
VERSION     DAZKAO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.181"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8H8_002652"
     CDS             127..579
                     /locus_tag="ACG8H8_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987667.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8H8_002653"
     CDS             593..1282
                     /locus_tag="ACG8H8_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987668.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8H8_002654"
     CDS             1407..3758
                     /locus_tag="ACG8H8_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP8987669.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1308 a          501 c          787 g         1247 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atg
//
LOCUS       DAZKAO010000179         3825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000179 DAZKAO010000000
VERSION     DAZKAO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.182"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..332
                     /locus_tag="ACG8H8_002655"
     CDS             <1..332
                     /locus_tag="ACG8H8_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987670.1"
                     /translation="LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSIS
                     LCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNF
                     YFYRNKL"
     gene            complement(458..1135)
                     /gene="nth"
                     /locus_tag="ACG8H8_002656"
     CDS             complement(458..1135)
                     /gene="nth"
                     /locus_tag="ACG8H8_002656"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP8987671.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1142..1840)
                     /locus_tag="ACG8H8_002657"
     CDS             complement(1142..1840)
                     /locus_tag="ACG8H8_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP8987672.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2108..3292
                     /locus_tag="ACG8H8_002658"
     CDS             2108..3292
                     /locus_tag="ACG8H8_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP8987673.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3298..3741)
                     /locus_tag="ACG8H8_002659"
     CDS             complement(3298..3741)
                     /locus_tag="ACG8H8_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP8987674.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          991 a          756 c          633 g         1445 t
ORIGIN      
        1 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
       61 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
      121 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
      181 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
      241 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
      301 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      361 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      421 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      481 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      541 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      601 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      661 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      721 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      781 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      841 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      901 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      961 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
     1021 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
     1081 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
     1141 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
     1201 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1261 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1321 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1381 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1441 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1501 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1561 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1621 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1681 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1741 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1801 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1861 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1921 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1981 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     2041 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     2101 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     2161 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     2221 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2281 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2341 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2401 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2461 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2521 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2581 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2641 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2701 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2761 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2821 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2881 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2941 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     3001 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     3061 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     3121 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     3181 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3241 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3301 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3361 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3421 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3481 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3541 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3601 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3661 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3721 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3781 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaat
//
LOCUS       DAZKAO010000180         3810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000180 DAZKAO010000000
VERSION     DAZKAO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.183"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            329..1018
                     /locus_tag="ACG8H8_002660"
     CDS             329..1018
                     /locus_tag="ACG8H8_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8987675.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1005..2213
                     /locus_tag="ACG8H8_002661"
     CDS             1005..2213
                     /locus_tag="ACG8H8_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP8987676.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2507..3778
                     /gene="serS"
                     /locus_tag="ACG8H8_002662"
     CDS             2507..3778
                     /gene="serS"
                     /locus_tag="ACG8H8_002662"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP8987677.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1252 a          656 c          774 g         1128 t
ORIGIN      
        1 tttttattat ttaataagtt attggtgttc ttttgaacaa ataataatat atcaacatca
       61 catgtgaaag tcaataagaa aatgcttatt tatcaacatt tctagcttac atgatcattg
      121 gttagttctg gtcctttttc gttcgtcttt ataactccga gagacgtaaa atccttttgt
      181 tttcagttta atgttcatgt tcttgtctta ttcacaccaa ctagatgtct caccttctaa
      241 aattttcctt aacaaagtgc tgcttttatc tatttctgct tttttttgct agaataatag
      301 taacaatatg actctggagg atttatttat gaaaattttg attgctgacg atgataaaga
      361 aatcgttgaa ttattaagta tctatgttca caatgagggg tatgaagcag taaaggctta
      421 tgatgggaaa gaagcattat ctaaaatcag aacaatccct gatattgaat tacttatttt
      481 agatatcatg atgcctgaga tggatgggat gcaagtagtg aaagagctac gaaaagaatc
      541 ccaaatacca atcattatgc tgacagcaaa aactactgat atggataaga tcaaaggact
      601 ggtcgctggt gcagatgatt atgtgaccaa gccatttaat ccgttagaag taatggctag
      661 agtaaagtct ttattacggc gtacaaaaat gcagattgcc ccagatcagc ctgatgtatt
      721 agaagtaaat gcattaatca tcaataaaga ctctcatgaa gttaaaacaa tcgatgggaa
      781 agaaatccag ttaacagctt tggaattcgg tatcctctac ttgcttgcta gtcatcctaa
      841 tcgagtattt agcgcggatg aaatttttga gcgtgtttgg aaacaagaaa gtattgtctc
      901 tgctaaaact gtcatggttc acgttagtca tttaagagat aagattgaag aagcaactgg
      961 tggtgaaaaa gtcattcaaa ccgtttgggg agtgggctat aaaattgacg caagatagta
     1021 aacgagcaac tgaaaaacgc cgtcggatca atttgacttc aaaagaaatc agcgaattgt
     1081 tagcggaagg aatcatcacg attatccttt tgcttctatt aaatgtttct attctggttg
     1141 ttatcagttc ggtgatcaac agttcacctt ctttgacgaa tgcgatttgg gattcaaaga
     1201 atatttttgc agaacgattg aacactgatt tgttctggaa cggccggaat tttattattc
     1261 cctttttctt tctattagat attggtgttt tgtattggcg cttgattcga agatatcgcc
     1321 aaatgcaatt gcggcatatc atcagcgaac ttcactacat cgcaaacgga aattatgatc
     1381 atcgtatccc ttttgaactg agtggtgact taagccgagt ggtaacaagt atcaatggat
     1441 tggtggatag taccgtagca gcgattgaag acgaacgtaa aatcgaaaaa tcaaaagatg
     1501 aattgatcac gaatgtcagt catgatatcc gtaccccact cacgtccatc attggatacc
     1561 taggactgat agaagatggc cagtatcact cagaagaaga tcttttaaag tatacgcata
     1621 ctgcttatat caaagcaaag cagatgaaat ctttagtgga cgacttattt gaatacacta
     1681 aggtacgcca gcctgctgtc ccggtgaatt tttctgcctt tgacatgatc cagttgatcg
     1741 aacagttagc agctgatttt gagttggaag cttctaaaaa aaatatccaa atacttgttc
     1801 aatcaaaagt agactccctt atcatggatg gcgataccga aaaacttgta cgtgtcttca
     1861 ataatctttt gacgaatgcg ttgaaatacg gaaaaggagc aacgaagatc gtaatcgaag
     1921 tagaacgtat cggttcagaa gttgttgcaa cagtcaaaaa taacggagca atgatcccac
     1981 aacaggcaat cgataatcta ttcgaccgtt tttatcgtgt agaagaatct cgttcacaag
     2041 caactggagg aactggactt ggtttagcaa tcgctcaaag cattgtggct ttgcacggcg
     2101 gatacattta tgcaaaatca gataagcaat ggacttcttt catcatgcac cttccaatca
     2161 agaaaaatga aaaattacct attgatacac aggaagttat tgacgaaagc tgaaatattc
     2221 gctattctta ttacaattat tagaataaat gaaaaacaca gaacccagta cactttattc
     2281 tttttcagag agctgatggc aggtgtaaat cagcaaagga taatttggaa gttccagttc
     2341 gtttacgtgc cctttcgaaa taagaaaggt cggttcaaca gccgttatgt gttcaacaag
     2401 tgcagctgac gctgaacttg ggtggtaccg cgaatttcag actgattcgt cccaagaata
     2461 atgggatgga tcagtctttt tttattttca aaaaggagga aatcatatgt tagatgtaaa
     2521 aatgatgcga caaaatattg aagaagtaca agaaaaattg atgactcggg gcgtaaaaaa
     2581 agaaacatta gctgaattca tggaactaga tgaaaatcgt cgtaagctac tagtcaaaag
     2641 tgaagaatta aaaaaatatc gaaatgatgt ttctgctgag atcgcccaat tgaagcgtgc
     2701 aaaagaagat gcttctgcta aaatcgctga aatgaaagaa gtcggtggca acatcaaagc
     2761 attggatact gaaattgctg aagtggatga aaaattgaaa caaattgcaa ctactttgcc
     2821 aaaccttccc catgattctg tgccagttgg aacagatgaa gaggacaatg tagaaatccg
     2881 tcgttggagc gaaccacgta catttgcttt tgaaccaaaa ccacactggg aaattgcaga
     2941 aaatcttgat attttagatt ttgaacgtgg cgcaaaagta gccggtagcc gttttgtcta
     3001 ttacaaagga ttaggtgctc gattagaacg tgctgtctac aacttcatgc ttgatcagca
     3061 tatttacgaa cacggctata ctgaaatgat cactccttat atcgtcaaca gtaaagcgat
     3121 gtttggtacg ggacaatttc caaaattcaa agaagatgtt ttccaattgg agaatactga
     3181 tctaacatta atcccaacag ctgaagtacc attaactaac tattataatg atgaaatttt
     3241 agatggtgca gatttaccta tttatttcac tgcactaagc cctgcttttc gttctgaagc
     3301 cggaagtgct ggtcgggata cacgaggact gattcgtctt caccaattca ataaagtaga
     3361 aatggtcaaa ttttcggatg tagagcattc ttacgaagaa ttggaaaaaa tgacagctga
     3421 tgcagaagat atcttgaaaa aattgcattt gccttatcgt gtgatggcct tatcaactgg
     3481 agacatgggc ttttctgcag ctaaaactta tgacttagaa gtatggatac ctgcacaaga
     3541 tacttatcgt gaaatcagtt cttgttctaa ctgtgaagat ttccaagcac gccgcgcaat
     3601 gatccgttat cgcgataatg atggaaaaat ccactatact catactctga atggttcagg
     3661 acttgcagtt ggccgtacag tagctgccat tttggaaaac taccaaaatg aagatggttc
     3721 tgtcacagtt ccagaagcgc tcgttcctta tatggggaat ctgaaagtta ttaaataaag
     3781 aggctgtgaa aaagccgctt agtttcgagt
//
LOCUS       DAZKAO010000181         3762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000181 DAZKAO010000000
VERSION     DAZKAO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.184"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..713
                     /locus_tag="ACG8H8_002663"
     CDS             111..713
                     /locus_tag="ACG8H8_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFP8987678.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            1068..1304
                     /locus_tag="ACG8H8_002664"
     CDS             1068..1304
                     /locus_tag="ACG8H8_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFP8987679.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1301..1714
                     /locus_tag="ACG8H8_002665"
     CDS             1301..1714
                     /locus_tag="ACG8H8_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP8987680.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            2257..3183
                     /locus_tag="ACG8H8_002666"
     CDS             2257..3183
                     /locus_tag="ACG8H8_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFP8987681.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(3180..>3762)
                     /locus_tag="ACG8H8_002667"
     CDS             complement(3180..>3762)
                     /locus_tag="ACG8H8_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987682.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1375 a          501 c          674 g         1212 t
ORIGIN      
        1 tatttatgta aaacaacacg aataattatt gcttcgtaaa gttatatgag tataagttaa
       61 tttactggag gtagtgcaat gcttttttct gaaagatttg gttttaaaaa atggaggata
      121 tcatactgag agatagattg acagaccctc ttaaaaatag tttagcaaat gtgatttatg
      181 atatattacg agaggctgat aaagtttata gtaattataa tggaaaagtc tcatgggtaa
      241 tatattcttt tttaattaaa gagtttttta aagaacagat aaataatagc tatgagttgg
      301 gttatcattt tactgtttat tatgaagata ttatcaagag gttagaagac ttggagtggt
      361 atgcttatta tgatttatta gaggtttttg caagtgctaa aatcttggat aaaaagaaaa
      421 gagaggaagt aaataaaatt ctggagtcgg aaaattctgg ttacagatta agcgaaagca
      481 atgaatttat tccaatcaca gatgatattt ctattaagtc tataaatgct gtatctgaat
      541 caccatttaa ttacgcaaaa aatcatatcc ataaatctct tacatatata agcgagaagg
      601 aaaatcaaga ttataatact gtagtgagag aagcaattag tgctgtcgaa agttgtctta
      661 tcgaattttc aggattacca gctaataaaa aaatactttg ggaactgcta taaaagcaat
      721 taaagacaaa tcgaataaca atgtcgatct tacattttta aaaccatttg agacaatgta
      781 tggtttggcg agtaataatg gaatacgaca tgcagaaaat gaaaaaacaa ttgtctcaga
      841 tttacctgat gcagttttgg tgttaacaac atgttcagcc gtaattaatt atttgggagt
      901 aaaaatagca tatgactcca ataaatcatg aaaagttaaa attcatccaa gagctacatt
      961 ataactatta atttccaact ctttaaaagt ttagtaattg aaggaagtaa taagggtgat
     1021 tgaacaatac ggatatacgg tgtatactta tcgtaggagt tgatgatatg aatattaaaa
     1081 aacgcaaaat cagagaagta ggtaattcaa tagttgtaac tttgtcaaaa gagagtttat
     1141 tgcaaaaagg tttgaaacct ggagatacta tttttattga tgaagataaa atgatggaag
     1201 ctattgtaaa agaggaaagc aagatagatc ttgaaattga tatgtatgtt aaccaagctt
     1261 tatctgagta tgatgcagct ttcaaggaat tagttgaaaa atgatctatc taagtgaaaa
     1321 agatattgta aaattgaatg ttattcaaat aaagaaatat tcaccgcgag aaacaatcgg
     1381 agtcattgac aaaaatgcgc tgagtatggc tgtaaatcaa cctaaacagc atatttttgg
     1441 aaaagatctg tatcctgata aatttagcaa agcagctgtt ctgctaataa atctgatcaa
     1501 aaagcatcca tttcataatg ctaacaaacg tacagcattt ttagccgcgt atgtttttct
     1561 caagctaaat ggctattcac taaaaatgaa aaatcaggaa gtagtagaat ttgtagttga
     1621 aattgctacg tatcagggtg attttgatga cttaaaagag gcaacaacag atatactaaa
     1681 aaagaattca acaaagaccg attcaaaaaa gtagaagcaa actagggatt cagttaaatc
     1741 tgaagattta gtttgttctt tattttagtt tacataagat acattatgcg aagttcatta
     1801 ttttaaaagc ttaaataata aactttttta taataccaat ttatttatgt aaaccaatga
     1861 ccttaatcgc ttaagcaagc atgttatgta atactctact ctctccaaaa ctaatgacta
     1921 tctattttca aagaagctag aatgattatc tgatcaaatg ataatttata tttaattcct
     1981 ggtctactgc tcttctttta atcaattaat gtctaacgta ctgtacacga taaagtagga
     2041 agacttattt tcaacttcgg attcattttt ttaattcggt gaaaatcact aaattaagag
     2101 tttatccaag ttcattttag ctgagaatac gcttgaaaat aattttgatg aattttcctg
     2161 aattaaaatt tattaacttg ttgagtgata taattgtaat gtattaccaa atagttagca
     2221 cttagattag tgatggaaag gaatagtgag aacaagatga ctggtagaaa tcccaaagta
     2281 tttatatcat attcatggga gaataaggaa aataaagaat gggctagaga actagcagac
     2341 aaattacttg aaaacggtat tgatgcatat atagatcagt atgatttaag tttaggtgat
     2401 agattacctc agtttatgga gcagcaaata tccgattcag attatgttct aattatatgt
     2461 acaccagagt ataaaaaaaa ggcaaataat cgtacaagtg gtgttgggta tgaaggtcat
     2521 attatctcag cagaattgct ttctggaaaa aatgaacgta aatttatccc aataattaaa
     2581 gaaggacgtg caagcgaagt acttcctact tttcttgctg gaaagcttgg aattgattta
     2641 tcagatagcc agcattatga tgaaaactta gatgatttaa ttacaactat atttggtgtt
     2701 aagagaaaac cagcattagg tgatatacct aggcatatta aaggcagtcc tttactacct
     2761 atagaacata atgaaaataa agatattgaa atcttaggaa ttatcactga taaagtaact
     2821 gtacctaaaa tggatggctc aaggggttct gcattatata aaattccatt tagattaaat
     2881 agagtaccaa gtcagctttg gaaagagttg tttgttagaa attggaattc accaccacga
     2941 tatacaacaa tgcatagacc aggaattgct tctgttgtag acgatgaaat tttacttaat
     3001 ggaacaacta ttgaggaagt ccgggaatat catcgggaaa ctttaaaact ttgcattgaa
     3061 aaaagtaata ctcaagaaaa aaaaatattg ctagaggagg aacaaaaaaa agagagagag
     3121 aatgagcgag tcagatcaca taaagaaagt gttcagaaat tagcagatga attagagttc
     3181 tagttgatag tcagttcttt cttaattcga tacacagttt gtctagttac attatagtct
     3241 gttgctattt ttgaaatagc aacaccattc tcctggtcct gaacgatgct tctataaaca
     3301 aggcgacgtt gaggatcttt tgtgttcgca ctgtataact taggacgtcc tttatagacg
     3361 ccgtttgctt tagcaatctt tatcccttgt gcctggcgtc gttttgattc agtacgctct
     3421 tgttcagcaa tcatggccaa gatttgaatg attaggtctt tgataaattt atccaggaga
     3481 ggatttccga ttgcttcagc catgatcggt aaattagtga taataagtcg cacatttttc
     3541 ttctttagat aatttactga atcgataatc tcgtcataat tacgtccaag tcgatcgata
     3601 gcctctacaa taaaaatatc ttggtctcta acaaaatcta gaacttcctg aaaaatcggt
     3661 cgatgagtaa tagtcgctcc agattgtttc tcgatgaaaa ttttttcagc accaaatttt
     3721 ctcatttcct cgatttgacg ttgttcattt tgatcgatcg at
//
LOCUS       DAZKAO010000182         3660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000182 DAZKAO010000000
VERSION     DAZKAO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.185"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACG8H8_002668"
     CDS             complement(<1..2303)
                     /locus_tag="ACG8H8_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8987683.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACG8H8_002669"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACG8H8_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1044 a          804 c          565 g         1247 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caaactaact atccatcaat aacgtgtttt attttttaaa
     3601 atttctataa aaaaacaatc attcttaaaa agaatatata aaaataacaa taacctttgt
//
LOCUS       DAZKAO010000183         3627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000183 DAZKAO010000000
VERSION     DAZKAO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.187"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..100)
                     /locus_tag="ACG8H8_002670"
     CDS             complement(<1..100)
                     /locus_tag="ACG8H8_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP8987684.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNISYREVQE"
     gene            176..505
                     /locus_tag="ACG8H8_002671"
                     /pseudo
     CDS             176..505
                     /locus_tag="ACG8H8_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            648..869
                     /locus_tag="ACG8H8_002672"
     CDS             648..869
                     /locus_tag="ACG8H8_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987685.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            885..1727
                     /locus_tag="ACG8H8_002673"
     CDS             885..1727
                     /locus_tag="ACG8H8_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987686.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1790..3592
                     /locus_tag="ACG8H8_002674"
     CDS             1790..3592
                     /locus_tag="ACG8H8_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFP8987687.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1311 a          514 c          731 g         1071 t
ORIGIN      
        1 tttcttgaac ttcacgatag ctaatgttat aacgaagata gtagcccacg gctacaataa
       61 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct tctgctatct
      121 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aaccaacgac
      181 agaataccaa atttcagaga ttaacaactt actggagaaa aagcaaaaag aactcatcaa
      241 gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg agctgagaga
      301 caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa
      361 tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg atgataagct
      421 tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca tatccttcaa
      481 atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag
      541 agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta ttgttattat
      601 aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag
      661 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
      721 ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta accttagatg
      781 agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt atattgcctg
      841 atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa atcataacaa
      901 taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa ccacaacctc
      961 atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt ttattaaaag
     1021 aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat tggtgcattg
     1081 acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca atccataaac
     1141 aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt gtaggtgcaa
     1201 gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca tttcctcttc
     1261 ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca aattcaacac
     1321 atctttctca atataagagc aggttgtctt tatatgattt ggctaaagta attctggaga
     1381 ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat attatcgcaa
     1441 ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca tatcataatg
     1501 tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa aatactcttc
     1561 cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga agttttgatt
     1621 acgaggcatt taacgaatgt gttggaaaac tactagatga aaataatata catattcctt
     1681 ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat ataagaaaaa
     1741 gacatcgtaa aagtgattat ggataaaatt aatgggggag gaaataatag tgggattatt
     1801 tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc aactggcttc
     1861 tagtctattt cgtactggta aagtttttgc tgaaaaattg gaagtggaca tatcaaatgt
     1921 agatgagttt cgtaaaactt ttatcgcttt tgatgtggaa acaactggcc taaattcttc
     1981 ttcagataga attgtagaga ttggtgcggt gctatttaaa gaaggagttc caattcaatc
     2041 attcggaact cttgtcaatc caaaagttag aattgcagcc tcagcttcgg caataaataa
     2101 tataactaat gaaatgctaa actctgctcc aaatgagcag gaagtttacc cccagttggt
     2161 agaattttta ggagatgcac tagatggaaa aatcactatg tgtgcacaca atgcaagttt
     2221 cgattttgga tttttaagta acacattttc tagacttggg tatgatgcta aaattaagta
     2281 tgttgatact ctgagtctct ctagaaaaca tataaaaggc attgtaaatc ataagcaaga
     2341 gactgtagcg gaatatttcg gattaataaa tgacactgcc catagagcgg aatcagatgc
     2401 agatatatgc ggaaagattt tttggggtgt tttagatatt attgaggagt cgttggaaga
     2461 acaaaaaaag caaatagaaa agatgactcc agacataaaa gaacttgaag cttgtgcttt
     2521 tattcaagat attattgaga aaaaaggatg cgacaccagt tggatcaggt atcgaaaaaa
     2581 cagtaataat tatgtcgatg taacttgtct atatacattc ttaaagttta agtttgctaa
     2641 gaagggaaat tacatcatta ttagaaaaga tgctacaaat ggaattgatt tacctataga
     2701 accttgtact gtaagtgaag gcggtacaac aaatgtgcga gtctattttc gtagcccctt
     2761 tgacttaacg cctctttcaa attatatttt cagtgcttat agcgattgtt ataattctat
     2821 gaatgaatat atgagtgtta gtaattacac taaaaaagaa gcagagcaaa gccttagtat
     2881 gctgaaatct atttcaaaat cagcgatgaa agagttatta ttcgatgcaa aaaatcgtgg
     2941 atatgatgag actgaagtta acctaaatat tgaaaaagtg atatctagag aagatgtaat
     3001 tattaacggg ttgcataatc gagtttcatt aaacaagata cgaaatatag ggaattggga
     3061 gaaaggattt gatgcaggtt atccatattg ggaacgtgga gaaactgcta ggaaaaatgg
     3121 caaagtagat gaagctattg cactttttga taaagctcga tataacggct atgatgctcc
     3181 tgcgctatat gattcttatg ctaaagctta tcgaaaaatt aaggattacg ataatgaaat
     3241 tctcattctt gatgaaggaa ttatgcgtaa aacacggcac gatgttggta ctttagaggc
     3301 tcgtagggat aaggcaatta agctattatt tgctaaacaa gaggcagagc ggatagctaa
     3361 ggaaaaatct gattttctaa aagcgaacaa aaaagaggat gcgattagtg agcctagcaa
     3421 gaagcgtgga agacagatta ttcaaatgac tgatgatgga acaataataa aagaattcga
     3481 tactatagct tctgctgtaa aagaagttgg cgttaactca aaaagtatta gggatgctgc
     3541 aaatggagtt caaaaacatg ctggaggata ctgctggaaa tacaaagatt agcagataat
     3601 ctaagataac ctaggatctt cttaata
//
LOCUS       DAZKAO010000184         3355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000184 DAZKAO010000000
VERSION     DAZKAO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.189"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACG8H8_002675"
     CDS             <1..188
                     /locus_tag="ACG8H8_002675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987688.1"
                     /translation="LEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIED
                     WAIFMAKEGKEMFQNKG"
     gene            201..476
                     /locus_tag="ACG8H8_002676"
                     /pseudo
     CDS             201..476
                     /locus_tag="ACG8H8_002676"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            503..802
                     /locus_tag="ACG8H8_002677"
     CDS             503..802
                     /locus_tag="ACG8H8_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFP8987689.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="ACG8H8_002678"
     CDS             976..1527
                     /locus_tag="ACG8H8_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP8987690.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="ACG8H8_002679"
     CDS             1673..1966
                     /locus_tag="ACG8H8_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP8987691.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="ACG8H8_002680"
     CDS             1959..2255
                     /locus_tag="ACG8H8_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987692.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="ACG8H8_002681"
     CDS             2330..3328
                     /locus_tag="ACG8H8_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP8987693.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1264 a          459 c          663 g          969 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat atcaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaa
//
LOCUS       DAZKAO010000185         3338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000185 DAZKAO010000000
VERSION     DAZKAO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.190"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..295
                     /locus_tag="ACG8H8_002682"
     CDS             <1..295
                     /locus_tag="ACG8H8_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP8987694.1"
                     /translation="NSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIET
                     ALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            268..945
                     /locus_tag="ACG8H8_002683"
     CDS             268..945
                     /locus_tag="ACG8H8_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP8987695.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            896..2077
                     /locus_tag="ACG8H8_002684"
     CDS             896..2077
                     /locus_tag="ACG8H8_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP8987696.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2090..3067
                     /locus_tag="ACG8H8_002685"
     CDS             2090..3067
                     /locus_tag="ACG8H8_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8987697.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3067..>3338
                     /locus_tag="ACG8H8_002686"
     CDS             3067..>3338
                     /locus_tag="ACG8H8_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP8987698.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKP"
BASE COUNT         1253 a          414 c          635 g         1036 t
ORIGIN      
        1 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
       61 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
      121 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
      181 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
      241 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
      301 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
      361 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
      421 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
      481 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
      541 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
      601 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
      661 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
      721 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
      781 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
      841 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
      901 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
      961 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
     1021 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
     1081 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
     1141 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
     1201 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
     1261 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
     1321 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
     1381 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
     1441 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
     1501 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
     1561 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
     1621 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
     1681 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
     1741 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
     1801 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
     1861 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
     1921 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
     1981 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
     2041 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
     2101 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
     2161 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
     2221 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
     2281 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
     2341 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
     2401 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
     2461 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
     2521 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
     2581 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
     2641 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
     2701 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
     2761 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
     2821 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
     2881 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
     2941 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
     3001 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
     3061 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
     3121 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
     3181 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
     3241 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
     3301 attgttgaaa aagttgatta catattttta gaaaaacc
//
LOCUS       DAZKAO010000186         3332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000186 DAZKAO010000000
VERSION     DAZKAO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.191"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8H8_002687"
     CDS             complement(451..1761)
                     /locus_tag="ACG8H8_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP8987699.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8H8_002688"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8H8_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8987700.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1030 a          676 c          514 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc at
//
LOCUS       DAZKAO010000187         3266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000187 DAZKAO010000000
VERSION     DAZKAO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.192"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /locus_tag="ACG8H8_002689"
     CDS             <1..702
                     /locus_tag="ACG8H8_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP8987701.1"
                     /translation="FFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            692..1432
                     /locus_tag="ACG8H8_002690"
     CDS             692..1432
                     /locus_tag="ACG8H8_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP8987702.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1429..>3266
                     /locus_tag="ACG8H8_002691"
     CDS             1429..>3266
                     /locus_tag="ACG8H8_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP8987703.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1226 a          397 c          632 g         1011 t
ORIGIN      
        1 tttttcaaag atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata
       61 aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa tattgttact
      121 ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct
      181 attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc
      241 aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat
      301 actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat
      361 tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt
      421 ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg
      481 tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat
      541 atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct
      601 gattcaatgg ttcggaagat taccacaaaa attaaaacag aattgacaag acaaatgaaa
      661 cgagatgctg gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat
      721 taatcaacat tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc
      781 agctagtcga gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa
      841 aggctattac aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga
      901 tatggtgcag gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga
      961 ttttattctt tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga
     1021 atttaaagga acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg
     1081 accatttaag gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat
     1141 ttataaccca gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc
     1201 ttctttttat attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat
     1261 agattctaaa ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca
     1321 ggtcacctta tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac
     1381 gaaaaatatt cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga
     1441 atatatagtc ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt
     1501 acaagatgtg gaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct
     1561 ttaggaacta gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac
     1621 gatcaagaag agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat
     1681 acagtaacta tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt
     1741 gtggaagtgg actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat
     1801 aaaaaatctg acatacgact ttattctgat attacggctg tttctagcac aatctttgaa
     1861 gcgagaaatg ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc
     1921 ggagaaatta aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat
     1981 gttggaacga ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca
     2041 ggtataaaaa caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat
     2101 cgaatttatg gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca
     2161 gaacatgttc agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata
     2221 gctaaaaatt actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg
     2281 gttgaatttg ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat
     2341 tatggtctgt ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca
     2401 aaggttagtg gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga
     2461 gacattcaag ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat
     2521 tatgcaacga ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt
     2581 caaggcagtg caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt
     2641 attatggata atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg
     2701 attgcttttt ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt
     2761 atatttaatg ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct
     2821 tttaacaaga ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac
     2881 aaaaagaaaa taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca
     2941 attggaacga ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca
     3001 acccctattg aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg
     3061 atttctccta aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt
     3121 ggagacttag atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa
     3181 ggtggtggtc catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca
     3241 gatgcagaaa agagagcttg gagaat
//
LOCUS       DAZKAO010000188         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000188 DAZKAO010000000
VERSION     DAZKAO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.193"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8H8_002692"
     CDS             301..585
                     /locus_tag="ACG8H8_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987704.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8H8_002693"
     CDS             588..1004
                     /locus_tag="ACG8H8_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987705.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8H8_002694"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8H8_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8987706.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8H8_002695"
     CDS             2544..2630
                     /locus_tag="ACG8H8_002695"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP8987707.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8H8_002696"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8H8_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987708.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZKAO010000189         3182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000189 DAZKAO010000000
VERSION     DAZKAO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.194"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..855
                     /locus_tag="ACG8H8_002697"
     CDS             <1..855
                     /locus_tag="ACG8H8_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987709.1"
                     /translation="QDIEERKNAMHGQAKSAEELINQKRHEAKYTPEAMNREEIMLRK
                     RVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQ
                     GSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSSSTSQPKTVDVRENVQNSTSS
                     QGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKT
                     QKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNP
                     LFDSLLKK"
     gene            869..1066
                     /locus_tag="ACG8H8_002698"
     CDS             869..1066
                     /locus_tag="ACG8H8_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987710.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            1070..1681
                     /locus_tag="ACG8H8_002699"
     CDS             1070..1681
                     /locus_tag="ACG8H8_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP8987711.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1666..3057
                     /locus_tag="ACG8H8_002700"
     CDS             1666..3057
                     /locus_tag="ACG8H8_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP8987712.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT         1259 a          446 c          624 g          853 t
ORIGIN      
        1 caagatattg aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc tgaagaatta
       61 attaatcaga agcgtcatga agcgaaatac acgcctgaag caatgaatcg cgaggaaata
      121 atgttaagga aacgtgtaga agggcggaca ggcgctaata ttgacggaaa agaagcgttg
      181 accaaagaaa gaattcaaga agctaaaaat tcaaatacag gattagaaaa attagtcaaa
      241 gaaaatccac aaaattctac aagtagtcat ggtggccgat cagtagatgt ccgagaaaat
      301 attcaaggtt cactgaatgg tcatgttgga cggaatgttg atgtaagaga gaatttacaa
      361 agttcttcaa atgctcaagg tactaagaca attgatgttc gagaaaatct gcaaagtagc
      421 agtacaagtc aaccaaaaac agtagatgtt cgagaaaatg tacaaaattc tacaagtagt
      481 caagggttta aatcagtgga tgtccgagag aatgtgcaag gtagcagtgc aagtcaacca
      541 aaaacagtag atgttcgaga aaatgtacaa agttctacaa gtagtcaagg attcaaaaca
      601 gtagatgttc gggagaacgt tcaaaaagat agtggagaaa tgaaagaaaa aacacaaaaa
      661 atcaatatcg ttgaagacag aaagtcagcg aaaaaatttg attcaaatca tgaaacagtg
      721 attattgata gtgaaatccg tgaaaatgat aagggatcaa aaccaagacg aaggtttacc
      781 tatgaagaca acgagttatt cagagataca cttaatgatc ctaatccact tttcgatagt
      841 ttacttaaaa aataaagagg tgattgtcat gcaattaatt ctacagatta gtacacatga
      901 tttcttagaa cttgacgaat tatgtgaatc agtaaagaaa gcaacaaatg aaaaaatcac
      961 ggtcactaat tatattgaac tgatgattca agaagaatta aagatgaaaa atgaaataga
     1021 gagcgatttc aaatgttgct ctctagaaaa aaatgtgagg ggataatcaa tggatgataa
     1081 acaaaaaatg tacgaaaaaa atcgcaaaca tagtgaggcc ttaaattttc gtgtcgataa
     1141 aaagacttat cttgaactga cattgattgc aaaacaaagg ggaatttcta gagcagacgt
     1201 ttgccgtgag cggttaaagg gactcaaata tgaaccgccg ttgttagctg aagatgatag
     1261 ggtagtttta accaaacagt taaatcaaat tggtaacaat ttgaatcaag cggtaaaggc
     1321 attaaatatc attgctaaat attttagaga agaatctgaa gataaaaaag aaaagagcct
     1381 acattttagt tttgatgcta gccgttacaa catttttaaa gatgaacttg cgaatagcat
     1441 agcaaaaaag ttgatgatta gttatccaga tgacattttt ttggagtcca atcggagtgc
     1501 cccctctttt tttaaattat cagaagtaga aaaagaatat ttcgaatatt ggatagaaaa
     1561 gcaaactaga ccgggtacga aagaagagta ttcccaaaaa gttgatcgga atttaattca
     1621 ctcaattcag attattgcaa gtttaaaaga tgaggtgaaa caactttggg agctactata
     1681 acatcgaaag gacgcaagaa aaatttatcc tcgctattac aatatgcagc taatccttca
     1741 aaaaaagaaa aatgtgttgc agtctcagga ataaattgta gtgatgatag tgaggaagca
     1801 ctgcaagaaa tgaatttagt acgtaccaag atgaataaat ctaaagatat agcacaagcg
     1861 tttcatgtta ttcattcgtt tgataagagt acttctaaag agttaagtat tgataaaatg
     1921 cacgaaatag ctgtcgaatt tgcagagaaa gcttttccaa attcgcaaat cattgttgca
     1981 agtcacaatg ataaagatca ttttcatagt catcttgtca ttaataatat taacatggaa
     2041 acatgtaaaa ggttgcgtat tgaccctaaa gatttagaac taatgcgaaa aattaacgat
     2101 caagtcctca atgaacatgg tcttgaatca gtgaataatg agtactatga aaacttagaa
     2161 aagagaacca atctttattc aaacgatcct tctaagatga aatatggaaa aattaattat
     2221 caagagtttg tgcaaaatgc gatcgaagca acgttggaaa aatgcgaagt gaattctttt
     2281 gaagattttt cggatcattt ggcaaaagaa tataacattg aagtttatca attttcaaaa
     2341 acaaataaca aacttggcta tgttctttac aaacaaggtg taaatttcta tgagcgtaga
     2401 ggtgagttaa ttactttagg aagagataaa caatctagaa aagaaaaaag tcagtatgtc
     2461 gaacgtactt ttagtgcaag aaagttaggg aaaagatatt catttgaatc tgtttctgaa
     2521 caacttgaaa agaatttaga agaatcacat ataaaaaatt tttttaatat ggaaagagag
     2581 caagttatgt cgctaattag tggagtaaat tacaaaactg gtgagttaaa tgtaccgttt
     2641 gtggaattag attccatcgc acaaaagcaa tttagcgacc gtgaaggcga tgaaaaattc
     2701 attaaatatg atgacttctt gcgagaggta gacaagcatt ttgtaaatac aatgtcttta
     2761 gaagatgatc aagaatacac gattaaactt catacggatg tgaatcctca aactttacgt
     2821 agtgcgaaaa gtaaaacaat ttctattaaa aagactgatc agaaagagtt atttctaccg
     2881 gcaaagggtt ttgaaaattg ttctcataca ccttctgcta aacagattca ctatcaaatc
     2941 agtcgcataa tgaatgacga tgtaaaaatg tatcgtgcta aaaaggatta tgaattcaca
     3001 caacaaatga ttgaaaatca gcaaaaaagg caacaaaatt ttgaaatgga attctgaatg
     3061 ggcttgttag tggactttcc agttttttgc ctatgaagag ttgcttggaa agaagcagtt
     3121 tttaggacat acgccagcta cccatctact tgccagtcaa tccgccccgc tcacgctagg
     3181 ct
//
LOCUS       DAZKAO010000190         3095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000190 DAZKAO010000000
VERSION     DAZKAO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.196"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..516)
                     /locus_tag="ACG8H8_002701"
     CDS             complement(148..516)
                     /locus_tag="ACG8H8_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP8987713.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            666..1148
                     /locus_tag="ACG8H8_002702"
     CDS             666..1148
                     /locus_tag="ACG8H8_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP8987714.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1185..2195
                     /locus_tag="ACG8H8_002703"
     CDS             1185..2195
                     /locus_tag="ACG8H8_002703"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP8987715.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2310..3023)
                     /locus_tag="ACG8H8_002704"
     CDS             complement(2310..3023)
                     /locus_tag="ACG8H8_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987716.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1045 a          503 c          608 g          939 t
ORIGIN      
        1 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
       61 ggatgataaa aaggatagga ttgaacaaaa cctgtataag ctgatgaata gtttcctcgc
      121 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
      181 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
      241 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
      301 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
      361 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
      421 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
      481 tttgatctga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
      541 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
      601 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
      661 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
      721 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
      781 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
      841 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
      901 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
      961 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     1021 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     1081 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     1141 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     1201 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     1261 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     1321 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     1381 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg ccttggggag
     1441 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     1501 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     1561 ccactgcaca tggaaccaac cttattgatt taagaggaga gtacttggat gaaacgacag
     1621 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caaccttctg
     1681 aaaagtatca aaaagagtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     1741 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     1801 gtggctgtat tgatacgatc agtcatttaa tcggtacccc ttatggagat gtccaatctt
     1861 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaat tgtgagctat
     1921 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggatgg tttgatggca
     1981 gctcggggat catgttcggt agaagtgcag cagaggattt gttagattat aaaatgaagg
     2041 atgtatatga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     2101 gtcatgtacc gccacaactg acattgatca atggtgccta tgcagaagta gcggtagaag
     2161 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagttt
     2221 atcaaagacc aacaaatcta aatagaaaag aaaatgaaag aagctgacag tattttgcca
     2281 gcttcttttt gtatgatcta ttagaaattt taattatcca taactatcac attgatcagc
     2341 tgtttcacat catttggatt acctgtagga atagctacta cagatgcatt atcaataatt
     2401 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     2461 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     2521 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     2581 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtag ttcgaaatca
     2641 cccgatgtat ctctaaaagt attacctcta attgctagcc aaacacgtgc gtactcaatt
     2701 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaagttgtg
     2761 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     2821 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     2881 gtacttgatt tttcagctga actggtttgt ttgattgaac ttttgtctgt ggtcgcagat
     2941 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     3001 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     3061 ataatcctga atttttgaat ccgtaaaacc ttgtc
//
LOCUS       DAZKAO010000191         3031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000191 DAZKAO010000000
VERSION     DAZKAO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.197"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..865)
                     /locus_tag="ACG8H8_002705"
     CDS             complement(251..865)
                     /locus_tag="ACG8H8_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8987717.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1471..1848)
                     /locus_tag="ACG8H8_002706"
     CDS             complement(1471..1848)
                     /locus_tag="ACG8H8_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987718.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2148..3029)
                     /gene="galU"
                     /locus_tag="ACG8H8_002707"
     CDS             complement(2148..3029)
                     /gene="galU"
                     /locus_tag="ACG8H8_002707"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP8987719.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          635 c          467 g         1011 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1381 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1441 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1501 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1561 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1621 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1681 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1741 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1801 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1861 ccttttttta taattaaaaa attcgctttt ttattctact atgaatcttc tggcttggct
     1921 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     1981 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2041 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2101 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2161 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2221 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2281 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2341 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2401 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2461 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2521 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2581 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2641 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2701 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2761 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2821 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2881 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     2941 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3001 gcaggaatga ctgctttttt gaccttcact t
//
LOCUS       DAZKAO010000192         3022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000192 DAZKAO010000000
VERSION     DAZKAO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.198"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..647)
                     /locus_tag="ACG8H8_002708"
     CDS             complement(321..647)
                     /locus_tag="ACG8H8_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987720.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(659..859)
                     /locus_tag="ACG8H8_002709"
     CDS             complement(659..859)
                     /locus_tag="ACG8H8_002709"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987721.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            982..1584
                     /locus_tag="ACG8H8_002710"
     CDS             982..1584
                     /locus_tag="ACG8H8_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8987722.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1639..2805
                     /locus_tag="ACG8H8_002711"
     CDS             1639..2805
                     /locus_tag="ACG8H8_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987723.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT         1062 a          503 c          433 g         1024 t
ORIGIN      
        1 atttctatct tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt
       61 ttcatttacg tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt
      121 agaatagcct tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc
      181 ttctgatttt atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag
      241 tgttcccccg atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa
      301 tttaaaaaac gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct
      361 tttgaaaaca actccatttt ttctaataaa ataactggca atcccattct tttaaatttc
      421 aaaaaagttg tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt
      481 ctttctaatc cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca
      541 taatttttca attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt
      601 atttttctcc tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt
      661 agattttcaa atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac
      721 tttctttgct cccattttcg attttgacat atgttttccg atttatacct atcaattttg
      781 ctgcatcgtt tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt
      841 gatcattaag agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc
      901 ttaataagca cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg
      961 tggttttcat gctataatag tttggaattt actatagggg gcgttacttt gaatagaata
     1021 aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt
     1081 aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca
     1141 tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa
     1201 acaaattcca tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag
     1261 gacaaaatag tcaagaatat tgccagtctt gactcagatt attttactga attatctaat
     1321 ctatactttt ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt
     1381 ctttttttaa aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa
     1441 aagaagttta ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct
     1501 gagttatttg gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct
     1561 cgattaatat caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc
     1621 agattggaga gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat
     1681 agtgtccgct tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa
     1741 gggtttaaaa ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa
     1801 gataccggct atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg
     1861 ccttcttata ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc
     1921 ttgcatatac taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt
     1981 cagaatgcag tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac
     2041 agctctcaaa tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca
     2101 aatacaaatc taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa
     2161 ctaaaagctt tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc
     2221 cagcttttga ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac
     2281 atttattttg aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa
     2341 gagaagaatg caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc
     2401 gaacgaatat taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa
     2461 caagctcgag agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt
     2521 acctatgtaa atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat
     2581 atattgaaac gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg
     2641 cacactcacg ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga
     2701 ttgggtcata aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct
     2761 caacgaaatg cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt
     2821 tctcattacc agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt
     2881 ggtataacaa cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg
     2941 gttttttctt cgtatcattg aataagtttg aaaagccttg atttataaag gtttttccgt
     3001 aaatatattt tgttatattt ga
//
LOCUS       DAZKAO010000193         2998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000193 DAZKAO010000000
VERSION     DAZKAO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.199"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..151
                     /locus_tag="ACG8H8_002712"
     CDS             <1..151
                     /locus_tag="ACG8H8_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987724.1"
                     /translation="LINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEI
                     LSKIS"
     gene            161..817
                     /locus_tag="ACG8H8_002713"
     CDS             161..817
                     /locus_tag="ACG8H8_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987725.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            817..2193
                     /locus_tag="ACG8H8_002714"
     CDS             817..2193
                     /locus_tag="ACG8H8_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8987726.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2323..2736
                     /locus_tag="ACG8H8_002715"
     CDS             2323..2736
                     /locus_tag="ACG8H8_002715"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987727.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2761..2998)
                     /locus_tag="ACG8H8_002716"
                     /pseudo
     CDS             complement(2761..2998)
                     /locus_tag="ACG8H8_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1139 a          396 c          525 g          938 t
ORIGIN      
        1 tttaataaat aatagtagtt ttatgtcaaa tataacaaca cttggacaaa gttatttaat
       61 attaataagt attattgttt tatcagttgt atttgcttct tcattaatgt taatcaagaa
      121 gcctaaagaa atcttatcaa aaataagtta ggagaaaata atggatatat tagaaataaa
      181 gaatgtagca tatagttatg caaattctaa agaaaaagtt ttgtcaggag taaatcaaaa
      241 atttgaactt gggaagtttt atgcgatagt aggaaagtca ggaacaggaa aatctacact
      301 tctttcctta cttgctggac ttgataaacc tcaaacagga aaaatattgt ttaagaatga
      361 agatatacaa aataaaggat atagtaatca tagaaaaaat aatatatctt tagtatttca
      421 aaactataat ttaatagatt atttatcacc aattgaaaat attagattag taaataaatc
      481 agcagatgaa agtatcttgt ttgaactagg tttagataaa aaacaaataa aaagaaatgt
      541 tatgaaatta tctggtggac agcaacaaag agtagctatt gctagagcat tggtatcaga
      601 tgctccaata atactagctg atgagcctac tggtaatcta gacagtgtta ctgctggaga
      661 aataattaat atattaaaga cattagctaa agatagaaat aaatgtgtaa tagttgtaac
      721 acatagtaag gaagtagcag attctgcgga tatcatttta gaactaagtg gtaaaaagtt
      781 gaaaaaagta aataaaatga atttggaggt tgaataatga taaaaaatgc atttgcttat
      841 gtaactagaa aaagtttaaa atcattaatt atcatattag ttattttatc tatgtcaact
      901 ttaagtctca ttagtttatc tattaaagat gctacggaca gagcttcaaa agaaacattt
      961 gctaatataa caaatagttt ttctatggag ataaatagac aagtaaatcc aggaacacct
     1021 agaggtgggg gaaatgtaaa aggtgaagat attaaaaaga tatctcaaac agatagtata
     1081 gactcctatg taaaaagaat aaacagtgtt gcagatttag ttgattatga tattattgaa
     1141 actcaagaga ctcttgcgaa tcaatcacct gaaagagcaa agaattttaa aagaacagtt
     1201 atgttaactg gggttaacga ctcaacaaaa gaaacaaaat ttgtatcaga agcatataaa
     1261 ttagtagaag gaaagcattt agaaaatgaa gataaaaata aaatcttaat gcataaagat
     1321 ttagctgaaa aaaataacct taaagttgga gataagataa aaataaaatc taatttattt
     1381 gatgctgata atgagaaagg tgcagatgaa acagtagaag tagaaattaa aggtctattc
     1441 gatgggcata atagcggtgg agtaagtgca gcacaagaac tatacgaaaa tacattaatc
     1501 actgatattc atacagctgc taaagtttat ggtaataccg aagatacagc tgtataccaa
     1561 gatgcaacat tctttgtaaa aggtgataag aatcttgata gtgtaataaa agatcttggg
     1621 aaattagata taaattggag agaatataat ttgattaaaa gttcatcaaa ttaccctgca
     1681 ttacaacaat ctatatcagg tatttactca atttcaaata aattattttt tggctcatta
     1741 atatttgcag gagttgtagt ttcattatta ttattcttat ggatgaatgc tagaaagaaa
     1801 gaaatagcag tattactatc attaggtata tcaaaattag aaatttttgg tcaattccta
     1861 attgagatgg tatttatatc aatccccgca ttcgttggtt cctatttctt agctcaatat
     1921 acagctgaca agcttgggaa taatatattg aataaggtta ctggtgatat agctaaacaa
     1981 atagctaaac aatctgcatc tagccaatta ggtggtgggg cagaagctga aggatttaac
     2041 aagacattat caagtttaga tatcaatgta ttacctaaat tcatgattta tgtagttata
     2101 tttatgagtt tagtacttct tgtatcattg attatttctt cattcaatat attaagaagg
     2161 aatccaaaag aattattaat tgacaataat taaaattttc ggtgcttttt agcaccaaaa
     2221 atgagagtgt aatataaacc gtgtaagtaa ccatcaatcc taggcgggtg gttgcttcac
     2281 ggtttttatt tttctaaaat aagatcaaga ggtgattaca cgatggctag aaagaaaaga
     2341 aatcctgatg ctgagaagtt agctgaatcc attttgaata cttatcaacc cgaatctgta
     2401 gaggatatgc aagatgtttt aaaagatgtg tttggtccct tatttgaaaa aatgcttcaa
     2461 ggagaattga ataaccattt agggtatgac gcccattcta aagagccaaa gaaacatgac
     2521 aatcgacgaa acggttatgg caataagacg cttaaaacga gctttggtaa agtaaacatt
     2581 gatgttccca gagaccggga ggcttctttt gaacctgagt tgattcctaa tagaaaacga
     2641 gatgtctccg acatcgcaga ctgtagacaa aagctgttcc aaactcgtaa ctcgagcttg
     2701 gaacagcttt ttatctactt cgtttcgaat ttttgaacaa aaaaataaag gctccttgcc
     2761 tttccatagc caggtggcta atttctttaa attcattgca gcaaacacta gcatcgtatg
     2821 cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc atcccatgct tttcttttgc
     2881 atccgcaaat attcgttcaa tcgtttgttt ccgttttttg taaattgatt tattcagatc
     2941 tgttaggcga agatgttcca cttcatccat cagatctccc caaacatgtc tcgtgatg
//
LOCUS       DAZKAO010000194         2949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000194 DAZKAO010000000
VERSION     DAZKAO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.200"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG8H8_002717"
     CDS             complement(<1..209)
                     /locus_tag="ACG8H8_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987728.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRN"
     gene            complement(583..849)
                     /locus_tag="ACG8H8_002718"
     CDS             complement(583..849)
                     /locus_tag="ACG8H8_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8987729.1"
                     /translation="MKSNKSFNKVLELTETALATPEIKKDKNLCEILEKVKASAAKGE
                     FYYDYKKEFQPAISGFTIRNGFSTPKVLLELLAEVKTPKAWSGL"
     gene            complement(854..1069)
                     /locus_tag="ACG8H8_002719"
     CDS             complement(854..1069)
                     /locus_tag="ACG8H8_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP8987730.1"
                     /translation="MRKKLFSLALIGIFGLVVTNFGTKVDAATRSYGNGVYCNNSKCW
                     VNWGEAKENIAGIVISGWASGLAGMGH"
     gene            1600..2211
                     /locus_tag="ACG8H8_002720"
     CDS             1600..2211
                     /locus_tag="ACG8H8_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987731.1"
                     /translation="MAKIGYARVSSIDQNLDRQLELLSKCDKIFTDKASGKDTNREGF
                     QKMMEFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGKPKYREDDKRLQLAF
                     KLFLAGSTDKEVEEQTGINRRTFRRYRMKYGITSKLREKNKIK"
     gene            2312..>2949
                     /locus_tag="ACG8H8_002721"
     CDS             2312..>2949
                     /locus_tag="ACG8H8_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987732.1"
                     /translation="MEFINNTKEKILNSPYFNKVSYLFEESFMTYSIGAYRSSYITSY
                     VGFLQQIRQNIINYNENPYFSIIEKKENETEEKYKAKIENKWRGLNKNLIDVDKWENA
                     LISALNENTDTNILRLNDGDRTKLLYFKNIRNDAVHDKTNQITLAQLQSLWEFIIEYA
                     HRTRIGENKESFLKNYQEITEWYKQSGDIPENRLRVIEESFYLLTEKEKIEIL"
BASE COUNT         1118 a          438 c          454 g          939 t
ORIGIN      
        1 atatttctac ctaaacgatc taagcgccaa acgacgagtg tatcgccaga gcgaacaaaa
       61 tcaatagcca tttctaatcc tggtcgatta ctttttgccc cactcatatg atcagtaaaa
      121 attttttcac aaccaaattt atttaggctg tcttcttgta aatccaaatt ttgtaaacca
      181 gtagatactc gggcgtatcc tattttcaaa aaaaatcact ccttcattaa ttttattgta
      241 acataacttt ataccaacga tataaatgaa catagatttt gtgatgggtt tgtgaacact
      301 aaaagtatag aaaaatttaa atttaaggta aaaaaatgta gtgtccagaa atgcctgatt
      361 ttatgaacac tttgttcaac agtaactttt gctaatcata caagatccga agattatact
      421 cgctcatttt tccataaaaa agaagccgtt cttttgacga acgacctctc ttttgatttg
      481 tactatccaa atatattgta acattttaat ttctatattc catatgttcc ttaaaactca
      541 attagatact cttacaaaaa atagaatagc acttatcata actcaaagtc ccgaccatgc
      601 tttgggagtt tttacttcag caagcaactc caataaaacc ttcggtgtgg aaaagccgtt
      661 tctaatagtg aatccactaa ttgcaggttg aaattctttc ttgtaatcat aataaaattc
      721 acctttagca gcactagctt ttactttttc taaaatttca catagatttt tatctttttt
      781 aatttctggg gtggctaatg ctgtttcagt taattctaga actttgttga aagatttatt
      841 acttttcata gtattaatgt cccatacctg ccaaaccaga agcccagcca ctaataacga
      901 ttcctgcaat attctcttta gcttctcccc agttaaccca gcatttacta ttattacaat
      961 aaacaccatt accatatgaa cgcgtagctg catcaacttt tgtaccaaaa tttgtcacaa
     1021 ctaacccaaa tattccaata agagctaaac taaataattt ttttctcata aatcaatacc
     1081 tcctttttct aaaataatta taaatgattt ttagaaaaat ttgataatta tatcataaac
     1141 agttgttttg aaagcataat ggcaataaaa aatcattgtt aaaactaatc tcatttgtat
     1201 tcttcgggga attaattcat tccctagaaa atcgtgtgtc acacaataac gcgcataatt
     1261 tcctactttt ctcaaatatt ttgctacttg attttcatat tcttccattt ctggagtata
     1321 ccctattgag atctaatttt atatctatct gaacttaatt aagtgaacta ttgaaagaac
     1381 gctgaaatga gaaattaaaa caggatcaaa accttaaaag ttgttggccc ttctataaag
     1441 ctatagaata tttttcgaat ttttgtgtga caaatactga gaaaaatttt tgccatttaa
     1501 atataaaaat gacacttgga tttctaaggg gtcaacagag ttgggcaaca ggattttaaa
     1561 aatgatccaa atttttgaat tttaaaggag tttttttcta tggctaaaat tggatatgct
     1621 cgagtaagca gtattgatca aaatttagat agacagctag aattgttatc aaagtgtgat
     1681 aaaattttta ctgataaggc aagcgggaaa gataccaatc gtgaaggatt tcaaaaaatg
     1741 atggaattca tacgtgaagg tgatattgtc gttgtgacag aattaaaacg tcttgggcga
     1801 aataataaag agctaacaga aacgatgaat ttgattcagc tgaagaaagc gactctggaa
     1861 gttttgaatt taccagcgtt aacagggata actgatgata atttgcgtcg attacttaat
     1921 aacttaatta ttgaactata caagtaccag gcggaagaag aacgtcgata tattcgtgaa
     1981 acgcagcaac aaggtattgc gcttgctaaa gcaaaaggaa aatataaagg tgggaagcca
     2041 aagtatagag aagatgataa acgtctgcaa ttagcattta aattattttt agctggatct
     2101 acagataaag aagtagaaga gcaaacagga attaatcgaa gaacatttag aaggtacaga
     2161 atgaaatacg gaatcactag taaattgaga gagaaaaata agataaaata acttaaaaat
     2221 tcacagagat tgaaccgatt tcatattttt acttgagtct taattaacaa atataatcca
     2281 ttaatttaaa aattaagtaa ggatggagta aatggaattt ataaacaata ctaaagaaaa
     2341 aattttgaat agtccctact ttaataaggt gtcttattta tttgaagagt cttttatgac
     2401 ctattcaata ggagcgtata gatcatcgta cattacttcg tatgtaggct ttttacaaca
     2461 aataagacaa aatataataa attataatga aaatccttat ttcagtataa tagaaaagaa
     2521 agaaaatgag acagaagaaa aatataaagc aaagattgaa aacaagtgga gagggttaaa
     2581 caaaaatcta atcgatgttg ataaatggga aaatgctctt attagtgctt taaatgaaaa
     2641 taccgatact aatatattaa ggctgaacga tggggataga actaagcttt tatactttaa
     2701 aaatattaga aatgatgcag tgcatgataa aacaaatcaa atcactttag cacaattgca
     2761 atctttatgg gaattcataa tagaatatgc acataggact agaataggcg aaaacaagga
     2821 aagcttttta aaaaattacc aagaaattac cgaatggtat aagcaatctg gggatattcc
     2881 tgaaaataga ttaagagtaa tcgaagagtc tttttattta ttaacagaaa aagaaaagat
     2941 agaaattct
//
LOCUS       DAZKAO010000195         2877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000195 DAZKAO010000000
VERSION     DAZKAO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.201"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..819
                     /locus_tag="ACG8H8_002722"
     CDS             <1..819
                     /locus_tag="ACG8H8_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8987733.1"
                     /translation="NLVEISGMSKNTILSALDELQKVKLLVKRKNGFNKSKMQMFPNF
                     YYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTR
                     DKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLL
                     KQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFV
                     DHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            895..1113
                     /locus_tag="ACG8H8_002723"
     CDS             895..1113
                     /locus_tag="ACG8H8_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987734.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1100..>2877
                     /locus_tag="ACG8H8_002724"
     CDS             1100..>2877
                     /locus_tag="ACG8H8_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP8987735.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPA"
BASE COUNT         1074 a          389 c          542 g          872 t
ORIGIN      
        1 aatctcgtag aaatttcggg aatgtcaaaa aatacaatct tatcagcact agatgaactt
       61 caaaaagtta aacttcttgt gaaaagaaaa aatggcttca ataaaagtaa aatgcaaatg
      121 ttcccaaatt tttattattt gttaaagcct gttctgtgca aagatgatgt ttataaaatt
      181 aataagataa cccatcaaga aaatagacaa aaaaacttga tagttcaaaa tttgaactat
      241 ggaacaaatc aatattctgt tgatagttca aaatttgaac tatgcgacac atcaatgaaa
      301 acaagagaca aaaacaattt tgctgatagt tcaaaaaatg aactagaatt atataagtct
      361 tataaagata gtaaagatat taaagaatca cataatgatg atcttcttga atcactttca
      421 actaaaaata ataaattaga tgaagaattg atcgaaacat ttattgaaga aaatcttctt
      481 aaacaaatat ggggagaaag tattgtgaat tgcttaaaac tggctagcaa tagtgattat
      541 cgtcaattcg acaactggta caaaaaattc aaatatgcga ttagatctgc tgaaaaagaa
      601 caaaaaattc aactgaaaat tatctatgaa atttgtaata acaagtattt tgtagatcat
      661 gttagagaac agctatcaat gactttacgt gacttgatac gaagagcaaa aacagatcat
      721 cgtatcaaac aaaaagacga ttatatcttt gcttctctaa aaaataaagc gttagaacta
      781 attgaattgc aaatcagcga aggaatagat aagcaataag aatagaattg agtcaaatga
      841 aatggagggg tatctccact tcatttgaga actttgaata taggaggggt tattttgatt
      901 aaaaatcgtt taatagatca agtttggtgt ctagatcaaa atacagatag tctcgaatta
      961 atcgagacta tttgcttcaa cactatagtt ctcgatttaa gagctgaaaa tgataattgt
     1021 gtaactcatg tcataattaa tcaaaaaatg gctcagaagc tctctgagag cctcagaaaa
     1081 tgggctaagg gaggtaacga tgaaaataat tgaaaaaaag attgccaata aaaaaataaa
     1141 ttggttggtt gttaattttc ttgttctgat agctatgatt atttttacaa gtaatattgc
     1201 tcatgcgagt attttcaatt ggtttgatga cacaaaaagt caaacagagt ttttgaataa
     1261 ttctctttat agaccttacc taaaaaagta caatggagat ttccttagtc agttcgggat
     1321 ttggctcgga tgggctgtgg taaaaggcat gtttactgtg acagattcga ttcaaaatat
     1381 gattccagat gtgctggatt tgttcaattt cattgaaagt acagggctaa ataatgttta
     1441 tcaatcagcc gtaaatacaa ttgtcattgg attaatgatc ctttcattga tgtttgttgg
     1501 ttacaaaatg attactggaa aaggaacagt ggatttaaaa tcggttggaa tgaacgtagt
     1561 gatgtctata gcattaatac ttttaatgcc aaccatgatt tctagtggta ttcaatttgc
     1621 taaaacattt tataaggact ctactacgat tacaaattca gatgacggag tcgcttggtc
     1681 attgataaaa caaggagtaa cagatttagc atatattaat aaaacagatc agtactcaag
     1741 cattgataaa acggaggatc gaaataattt aacacgaaaa aattttcaac agacggatct
     1801 cacacaagtg ttaacggata aagtgattga taaattggaa aaagaaaatc caattgcgga
     1861 caatcttcgt tatgaattag ttgaaaattc taataatgaa tttgttgcta caaagttttc
     1921 tgacaacttc ttatcaacat tttctgatag cttgaaatca ggctattacc gataccaagc
     1981 aaatctatgg gggatttcaa ttgggttgac agcgttagca atcgcctatg tgttcagcgc
     2041 gtttgtgatc attacagcaa tactagaact tgcatttaaa cgtgtgttag gtgttcttgt
     2101 ttttgcaaca gatattgaga caggacagcg ttcaaaagtt gttctatcag atattctaca
     2161 atgttactta actgttgggt ttcaaggatt tgggttatca atgtttgcta tgtttatcaa
     2221 ttttctgaac tcaggacaag gtatctcaac aaatattttt ataaaaacaa ttgcttatat
     2281 ctgtgccgtt tttgttttaa tcaaaggcag tggaacagtc atgagatact ttggtgttga
     2341 tattggactc aaagaaggct atgggcaact tgcttctgct tttggaatgg gcgcaatgct
     2401 gtttagaaaa ggatcaaatg gatttaatcg tgctaaagga agcggagaag gagaagatcg
     2461 aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc
     2521 tttgggatat gcccatgaac gtggattatc cggtttagct tcagatggag ctacaatggc
     2581 tagtgaaaga gcaacaaaac catttaagag tatgcgtgat atagctaatg ataccaagaa
     2641 taagtttaaa gaaggtttag atgatggtac agtgtccgct attaataaaa atagcaaacc
     2701 tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattccg tgccaacacg
     2761 tctttcagat agaaaagatg gtgctaaagt tgaaaatgcc gatagaatta tgtctagttc
     2821 tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc ctgcaag
//
LOCUS       DAZKAO010000196         2828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000196 DAZKAO010000000
VERSION     DAZKAO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.202"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      87..261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            399..1991
                     /locus_tag="ACG8H8_002725"
     CDS             399..1991
                     /locus_tag="ACG8H8_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8987736.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2004..2744
                     /locus_tag="ACG8H8_002726"
     CDS             2004..2744
                     /locus_tag="ACG8H8_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8987737.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT          891 a          529 c          622 g          786 t
ORIGIN      
        1 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
       61 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
      121 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
      181 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
      241 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
      301 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
      361 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
      421 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
      481 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
      541 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
      601 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
      661 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
      721 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
      781 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
      841 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
      901 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
      961 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
     1021 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
     1081 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
     1141 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
     1201 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
     1261 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
     1321 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
     1381 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
     1441 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
     1501 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
     1561 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
     1621 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
     1681 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
     1741 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
     1801 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
     1861 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
     1921 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
     1981 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
     2041 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
     2101 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
     2161 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
     2221 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
     2281 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
     2341 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
     2401 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
     2461 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
     2521 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
     2581 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
     2641 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
     2701 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
     2761 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
     2821 tttctgaa
//
LOCUS       DAZKAO010000197         2812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000197 DAZKAO010000000
VERSION     DAZKAO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.203"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..226
                     /locus_tag="ACG8H8_002727"
     CDS             <1..226
                     /locus_tag="ACG8H8_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987738.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            226..1619
                     /locus_tag="ACG8H8_002728"
                     /pseudo
     CDS             226..1619
                     /locus_tag="ACG8H8_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1634..1840
                     /locus_tag="ACG8H8_002729"
     CDS             1634..1840
                     /locus_tag="ACG8H8_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987739.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1861..2457
                     /locus_tag="ACG8H8_002730"
     CDS             1861..2457
                     /locus_tag="ACG8H8_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFP8987740.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1015 a          390 c          560 g          847 t
ORIGIN      
        1 taaaacaact gaatttgata ttctacacgc tctaatgtcc acacctaata aagtcttttc
       61 tcgtagtgat ttgatagaga aagttaaggg aatagaattt gatggactag ataggagcat
      121 cgattctcat ataaaaaact taagacataa aattgagaat gatgctaaat cacctatgta
      181 tattttgaca gtccatggta caggatacag atttggtggc cactaatgag aaaaagaacg
      241 ataaaaactc aattagctgt ttccttttta gcaatcgcaa cattaataat aggatcgatt
      301 agtttagtcg cattaagttt gatgaacaat cattttagta aatacgtgga agagcggcaa
      361 gaagatttgt tgaatcagta tgtatataca attgatttat tgtggttaaa tagtggggaa
      421 acatggaata gtgaagaact tgcagcacta tctgagaaag tattagagaa taatatttat
      481 ttttctatag aagacgaaca aggcaacatg gtttgggaac ttactggaaa agatttgaag
      541 tcagcacagg agaaactgaa aaaaatgcat tgaaagtttc tgagaaaaac agtgtgaaac
      601 tagacgaaac aatagaagtg aaaaagaagt taattaatga tggaaacgag tttggaaaag
      661 taacgtttta ttatttcgga ccttttgctt atacagagca cgatgccttg tttatctcca
      721 gtatgaaaca aagtttaatg tatgttgcaa ttgcagctct tcttgtatca ttcatacttg
      781 cttcgtggat ctctgctaga ttaggattgc cactaaagca tgtaagcgat tttacacata
      841 aattaactcg tggggaatat gctgataaaa tcccacaaga aacttcaatt attgaaataa
      901 attccttaat tgattcccta aatgatttaa gtaatcagtt ggagaaacag catgggcttc
      961 gaaaaagatt aactaccgat atttcacatg aattaagaac tccattagct acgcttaaag
     1021 gcaatgtgga aggaatgatt gacggagtct ggaaaataac tcccgagcgc ctacaatctt
     1081 gttatgacga aatagatcgt ctaactcgtt taattggaaa tatagaaata atcaacaaaa
     1141 ttgaagcaaa atatgatcat ttaaataaaa ctgagtttaa catatataag ttgattgaat
     1201 ccgttattga aaatttcgcc agcaaaattg agtcaaaaaa ccttcatgtt gaaatccaag
     1261 gggataacat caatatatct gcagataaag ataaaatgaa ccaagttgtt atcaatcttt
     1321 tgaataatgc tattaaattt acgcaaaagg agggaaccat taagttttct ataagtaaaa
     1381 ataaagacca tgttctttta attgttgaag acaacggcat cggtatagaa aaagatcagc
     1441 aacttcatat ttttgataga ttttatatgg ctgacccctc cagaagtaga gctttagggg
     1501 ggcaaggtat cggattagct attgtaaaga gcgttattga agcgcataaa ggtagtataa
     1561 ctgtaaaaag taaattaggt ctaggaacta aatttgttat aaagttacca tttcaataaa
     1621 ggaggagagt tatttgtttc aaaaacagat aaaaattgct aaactagtat gtgaatgttg
     1681 ggcaaaaaaa atattttttc aactgatttc gatagatagt gtcgaaaaag ttcatttaga
     1741 tatcttatca aattctgtag taatcttttc taagaaggac ttatcaatag ctacaattaa
     1801 aaaagaagtt tttggattac aaatttatga gatgacctaa atgtgattaa ttaaaggagg
     1861 atgaagacag tgaaaaagat gacaaaatta gtatttgcaa gtcttggaat tattgtattt
     1921 ttaggagcgt gtagcaatca aagtgaatcg aataattcta atagcacaaa cgaggaatcg
     1981 acttctacag cgtctagtga aatgaatagt atggagggaa tgaatcacga aggtatggtt
     2041 ccttcttcaa tgaaagatgc tgcgaaccct aaattcccgg tgggaagtaa tgttatactg
     2101 ctaggcgatc atatggaagg gatgaaaggt gcaaatgctc aagttgtagg ggcctttgat
     2161 actacaatgt atgaagtaag ctatgagcca aaaactgggg gaccaatggt taacaatcat
     2221 cgatgggtag tacaagaaga gttaaaagat actgagactg tagcaaatga gggcgatact
     2281 gttatcttga atgccgatca catggatggg atgatgggag ctgaagcaaa agtagataaa
     2341 tctattacag gaactgttta cgttgttaat tacactccaa ccgatggtca agaagaagtt
     2401 aagaatcata tgtgggtaac tgaagatgag atggaatatg acgaaaataa tgagtaattt
     2461 tatatactaa accaacaagc tggtacatgg tcttcgtatt ctcaacatga ggaatatgag
     2521 ctctttgtta ataaggactg atgagttgtg caaaatcaaa tctgggtcag aacgaacctc
     2581 attctggctc agatttttgg ctcttcgaca tttagtgctg gtagagagac aactgaaaat
     2641 ttagtcataa ccagttaggc agagtgctta actggttttt gttcgcattt attattcttt
     2701 tgatattggc tgcttgccct tcaattttat ttggtttctt aatcaactct tcatataacc
     2761 ccaaatcata tattaatgag gtgcctccta ttcctataaa tctatctctt ct
//
LOCUS       DAZKAO010000198         2720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000198 DAZKAO010000000
VERSION     DAZKAO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.205"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACG8H8_002731"
     CDS             complement(<1..962)
                     /locus_tag="ACG8H8_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987741.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACG8H8_002732"
     CDS             1134..1268
                     /locus_tag="ACG8H8_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987742.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1896)
                     /locus_tag="ACG8H8_002733"
     CDS             complement(1261..1896)
                     /locus_tag="ACG8H8_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987743.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="ACG8H8_002734"
     CDS             complement(2009..2644)
                     /locus_tag="ACG8H8_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP8987744.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          781 a          457 c          403 g         1079 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct
//
LOCUS       DAZKAO010000199         2718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000199 DAZKAO010000000
VERSION     DAZKAO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.206"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1308
                     /locus_tag="ACG8H8_002735"
     CDS             <1..1308
                     /locus_tag="ACG8H8_002735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP8987745.1"
                     /translation="SSFLLKFRGEIPLRNLETFQHSAIIISVIFIALNILLGAYVFYN
                     RMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVILLSFVLGTLFLSIYRILVYK
                     LYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKVEAVILDNYYANIKNMIKDIDI
                     VYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRI
                     QPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSI
                     YKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPR
                     PERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLM
                     LDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            1324..2364
                     /locus_tag="ACG8H8_002736"
     CDS             1324..2364
                     /locus_tag="ACG8H8_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8987746.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2383..>2718
                     /locus_tag="ACG8H8_002737"
     CDS             2383..>2718
                     /locus_tag="ACG8H8_002737"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8987747.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRI"
BASE COUNT          944 a          421 c          505 g          848 t
ORIGIN      
        1 agtagtttct tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa
       61 cactcagcaa ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat
      121 gttttttata atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg
      181 atgacactag ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt
      241 gttatcttac tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta
      301 tataaacttt atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag
      361 gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa
      421 gcagtcattc ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt
      481 gtctatcttg cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact
      541 aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca
      601 aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct
      661 gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt
      721 gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc
      781 atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc
      841 tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca
      901 cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc
      961 ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc
     1021 gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt
     1081 atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt
     1141 tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa
     1201 accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg
     1261 atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta
     1321 aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt
     1381 ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta
     1441 aatgatggat caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa
     1501 cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata
     1561 agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg
     1621 atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt
     1681 tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt
     1741 acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat
     1801 gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac
     1861 caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat
     1921 gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt
     1981 gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat
     2041 tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta
     2101 aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag
     2161 aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt
     2221 gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca
     2281 ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg
     2341 gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat
     2401 tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa
     2461 attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa
     2521 gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc taatcctaag
     2581 aaaaatattt ttaaatattt aatagaagtg aataagttac taaaaaaaga atctttttct
     2641 atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc caaaaagaat
     2701 ggaatcagac agagaatt
//
LOCUS       DAZKAO010000200         2704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000200 DAZKAO010000000
VERSION     DAZKAO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.207"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8H8_002738"
     CDS             <1..607
                     /locus_tag="ACG8H8_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8987748.1"
                     /translation="KCIEENLHLPTNQHYSPSEKNTPSDKSTFDGDKSFPLPGEKFLQ
                     ELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIESLIEKEWLYQVWGDNIVNCLK
                     LVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEICNNKYFVDYVREQLSMTLRD
                     LIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            683..901
                     /locus_tag="ACG8H8_002739"
     CDS             683..901
                     /locus_tag="ACG8H8_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987749.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            888..>2704
                     /locus_tag="ACG8H8_002740"
     CDS             888..>2704
                     /locus_tag="ACG8H8_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP8987750.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPV"
BASE COUNT          969 a          358 c          540 g          837 t
ORIGIN      
        1 aaagtgcatt gaggaaaatt tacaccttcc gacaaatcaa cattacagtc caagtgaaaa
       61 aaatacacct tccgacaaat caacattcga tggtgataag tcattcccgc ttccaggtga
      121 aaaattttta caagaactta atcattcttc taaagatttt aaagatttta aagaatctca
      181 aaatgctgac tttattgatt cactttcagt tgaaaataaa aaattagatg aaatattaat
      241 tgaatcatta attgaaaaag aatggcttta tcaggtatgg ggggataata ttgtaaactg
      301 tctgaaatta gtgggtaata acgattacag tcaattttat gactggtata aaaaattcaa
      361 atatgcgatt aaatctgctg aaaaagaaca aaaaattcaa ctgaaaatta tctatgaaat
      421 ctgtaataac aagtattttg tagattatgt tagagaacag ctatcaatga ctttacgtga
      481 cttgatacga agagcaaaaa cagatcaccg tatcaaacaa aaagacgatt atatctttgc
      541 ttctctaaaa aataaagcgt tagaactaat tgaattgcaa atcagtgaag gaatagataa
      601 gcaataagaa tagaattgag tcaaatgaag tggaggggta tctccgcttc atttgagaac
      661 tttgaatata ggaggggtta ttttgattga aaatcgttta atagatcaag tttggtgtct
      721 agatcaaaat acagataatc tcgaattaat cgagactatt tgcttcaaca ctatagttct
      781 cgatttaaga tctgaaaatg acaattgtgt aactcatatc ataattgaca gaaaaacagc
      841 tcagaagctc tctgagagtc tcagaaaatg ggctgaggga ggtaataatg aaaatagtta
      901 aagaaaagat tgccagtaga aaaatacact ggttagcttt gagcatactt gttttaatgt
      961 ttatcgttgt tttcaccggt aatgttgtcc aagcaagcat tttcaattgg tttgatgaca
     1021 cggaaagtca aactgaattt ttgaataatc ctgtctatca atcgtattta aaaaaatata
     1081 acggaaattt ttacagtcag tttggattgt ggatagggtg gtctgttata aaatcgatgt
     1141 ttgcaattgt agatggtatt caaaatatga ttcccgaagt gttagatttg tttagcttta
     1201 ttgaaagtac gggaatgaat aaagtttatc aatcggtctt aagtacagtt gttattggtt
     1261 taatgatact ttcactgatg tttgttggtt acaaaatgat tactggaaaa ggaacagtgg
     1321 atttaaaatc ggttggaatg aacgtagtga tgtctgtagc attaattctt ttaatgccga
     1381 ctttgattgg atcaacagaa aaactttcag gaataatgtt tgcaaagact ctttataatg
     1441 acgcaactag tatcaataat caagatggcg gtgtagcatg gtcattgatt gagcagggag
     1501 tgacagattt agcttatatt aataagacag atcaatattc gacaattgat aaaacaaatg
     1561 accgaaataa tctaacaaaa gaaaatttct ggaaaacgga tcttacgcaa gtattgacag
     1621 ataatgtaat tgataaaatg gaaaaagaga acccaacagc tgataatctg cgttatgaat
     1681 tagttgaaaa ttctgataat gaatttgttg ctactaaatt ttcagataat tttttatcaa
     1741 cctttgttga tggtatcaaa tcaggatatt atcgctatca agcgaacctg tggggaattt
     1801 cttttggatt gattgcttta gcggtagcgt atgtttttag tgctttcatt ataattacag
     1861 caatactcga gttagcattt aaacgtgtac tcggagtttt agtatttgct actgacttag
     1921 aaacaggaca acgatcaaaa caagttttgt cagatatttt acagtgctat ttgacagtcg
     1981 gatttcaagg gttcggttta tctatgtttg caatgttcat aaattaccta aatactggcg
     2041 aaggtatctc agcaaatatt tttataaaaa caattgctta tatctgtgct gtgtttgttt
     2101 taatcaaagg cagtggaaca gtcatgagat actttggtgt ggatattgga ctcaaagaag
     2161 gctatgggca acttgcttct gcttttggaa tgggtgcaat gctattccgt aaaggaacag
     2221 gtggtatgaa ccggtctaaa ggtgggaaaa ataatggtgg aaacgatcaa gctgaagggg
     2281 aagaacgaaa acctgaacgc aactttggac aatcactttc taaaaaagct aataaaacgg
     2341 ggaaagcgct tgggtatgct catgaacgtg gattatcagg tttagcttca gatggatcta
     2401 caatggctag tgaaagagca acaaaaccat ttaagagtat gcgtgatata gctaatgata
     2461 ccaagagtaa gtttaaagaa ggtttagatg atggtactgt gtccgctatt aataaaaata
     2521 gcaaacctat gttagctaaa aataaagaag atgaaactgg aaaatatgct gattccatgc
     2581 caacacgtct ttcggataga aaagatggtg ctaaggttga aaatgccgat agtattatgt
     2641 ctagttctga acgtatgcgt gaagcaatga aaaataatgc tgaatcgaat aataatcctg
     2701 taag
//
LOCUS       DAZKAO010000201         2648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000201 DAZKAO010000000
VERSION     DAZKAO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.208"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..876
                     /locus_tag="ACG8H8_002741"
     CDS             <1..876
                     /locus_tag="ACG8H8_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8987751.1"
                     /translation="LTIFFVHRHEDVLRVAKKISGPIVRVKDFTREIAKGDYTSLSPE
                     KTDIKELDELISSVNALSVQLENQQDIRNQLSSDIAHEIRTPLTTLKGSLEAMIDGIW
                     EIIDDRIQSCYDEVNRITRLIGSIDKINEIESHQDSLNKISFDLYALAENISSNFEAL
                     FAKKNIYYALNGDSLFITADRDKISQVITNLLSNAVKFTPPEGTITLKIRNEKNQALL
                     TIADTGEGIHPKEINRIFERFYMSDLSRNSFLGGQGIGLSIVKSIIKAHNGTITVKSD
                     YGKGSTFTVILPVKR"
     gene            complement(1046..2218)
                     /locus_tag="ACG8H8_002742"
     CDS             complement(1046..2218)
                     /locus_tag="ACG8H8_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HFP8987752.1"
                     /translation="MGLLLAACGQEESTSSQNSSQQAIVSTSQETSESQSSSKEENKE
                     QTEAYYTSDTGSVTKVDAQTHEVVDTIAIDGSAHNVQISPDEKVIGVTIVPSEGHAED
                     SSGSNESMEMEHDEDSDTSMSNESMEMGQDEDSDTSMSNESDDEKGIAAFYDAETGEK
                     LAEVEVGSHPAHIVFTNDGNYAAVSNNGENTVSVIDVSTYEVVETIPTGEGPHGFRIA
                     EDGKTAYVANMGEDTVSVLDLESMEEVNRITVGSTPATTGITSDGQTLLVTLNGENAL
                     AIVDLTTNKVEKVAVGNGPIQLFVQHDDQYVFVANEGTEENPSNTVSKVDLNSNEVVE
                     TIEVGSGAHGVVISEDDKYTFVTNAFDDTVSVIENETSEVIVTVEVGETPNGITLK"
BASE COUNT          793 a          509 c          420 g          926 t
ORIGIN      
        1 ttaacaattt tttttgtgca tcgacatgaa gatgtactcc gggttgctaa aaagataagt
       61 ggacctatag tgagagtaaa ggattttaca agagaaattg ctaaaggaga ttacacaagt
      121 ttatctcctg aaaaaactga tatcaaggaa ctagatgaac tcatttcatc tgtaaatgct
      181 ctttctgttc aacttgagaa tcaacaagat atcagaaacc aattatcttc tgatatcgct
      241 catgagatca gaactccatt aacaacacta aaaggaagtc tggaagctat gatagatgga
      301 atatgggaaa taatagatga ccgaattcaa agttgttatg acgaggtgaa ccgtattacc
      361 cgccttatag gaagcattga taaaattaat gaaattgaaa gtcatcagga tagtttaaac
      421 aaaatatctt tcgatttata tgctctcgcg gaaaatatat cttctaattt tgaagctctt
      481 tttgctaaga aaaatattta ttatgcatta aatggggatt ctctattcat taccgctgat
      541 agggacaaaa taagtcaagt cattacaaat cttttatcca atgctgttaa atttacaccc
      601 ccagaaggaa caataactct aaaaattcgc aacgaaaaaa atcaagcttt attgactatc
      661 gcagatacgg gagaaggaat tcacccaaaa gaaattaatc gtatttttga gaggttttat
      721 atgtctgatc tttcaagaaa tagttttcta ggcgggcaag gaattggctt atctatcgta
      781 aaaagtatca taaaggctca taacggaacc ataacggtca aaagtgatta tggaaaaggt
      841 tctacattta ccgttattct tcctgtcaaa agatagtaat catcaaaaaa agcggttcac
      901 ttttatgtaa agtgaaccgc tttttcagct tcatgataaa gttttccatt taatttagga
      961 ctaattaaga tttttaaacg ttagaatatt tatccctttt cagaaatcat taagttgatt
     1021 ttttagttta acttaacact aataatcatt tcagagtaat cccatttggt gtttcaccaa
     1081 cctcaaccgt gacaataact tcactagtct cattctcaat aacactgacc gtatcatcga
     1141 atgcattcgt gacaaaggtg tatttatcgt cttcactaat gacaactcca tgtgcaccgc
     1201 tacccacttc gattgtttcg accacttcat tactatttaa atcaaccttt gaaacggtat
     1261 tggaaggatt ctcttcggta ccttcatttg ccacaaatac atattgatca tcatgttgaa
     1321 caaacaactg gataggacca tttccaactg ctactttctc aactttattg gttgtcagat
     1381 caacgattgc taacgcattt tctccattta atgttactaa gagcgtttgt ccatctgagg
     1441 taataccagt tgtagctgga gtactgccaa cagttattct attaacttct tccatacttt
     1501 ctaaatcaag gacgcttaca gtgtcttcac ccatattggc aacgtaagcc gtttttccgt
     1561 cttcagcgat tctaaaaccg tgtggacctt cgcctgttgg gattgtttca accacttcat
     1621 aagtagatac atcaatgact gaaacagtgt tctctccatt attggaaact gctgcatagt
     1681 tgccatcgtt agtgaaaacg atatgagcag ggtgactccc tacttcaact tctgcaagtt
     1741 tttcacctgt ttcagcatca taaaaagctg caataccttt ttcatcgtca ctttcattac
     1801 tcatactagt atcactatct tcgtcctgtc ccatctccat actttcattg ctcatactgg
     1861 tatcactatc ttcgtcgtgt tccatctcca tactttcatt actacctgaa ctgtcttcag
     1921 catgcccctc gctgggaaca attgttacac caatgacttt ttcatcaggt gaaatttgta
     1981 cattatgtgc ggatccatcg atggcaatcg tatccacaac ttcgtgcgtt tgagcatcta
     2041 cttttgtaac acttccagta tcggaagtgt aataggcttc tgtttgttcc ttattttctt
     2101 ctttagaact agattgagac tcactagttt cttgtgacgt tgatacaatt gcttgttgag
     2161 aagaattttg actacttgtt gactcttctt gaccacaagc tgcaagcaag agacccactg
     2221 acgacaatag tgctaaagtg attctttttt tcattttaat atcccctttt ttactacgta
     2281 ctttttactt ttcgtactat ttttattgct ctataattgc ctgaattatt cataattttt
     2341 ccgtaggttt ctgaaaagat tgtggtactg cgttcctgat ttctcttctt tactttcatg
     2401 gtggtgcata atttatcccc ttgctttgat ttattttatg tttctgtttt tataataata
     2461 tatgaatgtg ttgattttat gtagaaaaaa accatatcaa aaagccaaga tttgtatatg
     2521 atcttggctt tttgatatga atagttcatt tccggaatat atttttgaga aaactcagtt
     2581 cgagcatgaa acttcagact tacccaacta acttttcaat attttcttct tttgttggta
     2641 ttcttcgt
//
LOCUS       DAZKAO010000202         2602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000202 DAZKAO010000000
VERSION     DAZKAO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.209"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            946..1032
                     /locus_tag="ACG8H8_002743"
                     /pseudo
     CDS             946..1032
                     /locus_tag="ACG8H8_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            1586..1810
                     /locus_tag="ACG8H8_002744"
                     /pseudo
     CDS             1586..1810
                     /locus_tag="ACG8H8_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2070..2324
                     /locus_tag="ACG8H8_002745"
     CDS             2070..2324
                     /locus_tag="ACG8H8_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8987753.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2325..>2602
                     /locus_tag="ACG8H8_002746"
     CDS             2325..>2602
                     /locus_tag="ACG8H8_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8987754.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVG"
BASE COUNT          969 a          357 c          414 g          862 t
ORIGIN      
        1 aataaaattt agttttaaaa ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaat
       61 aaattaagaa ctaccttatg catataaaac atactaccat tactagtaat atatactacc
      121 ttatttcagt agtatatatt atattcttct tataagacaa ggctttaggc tagaaaacaa
      181 tagattatat actactaaaa aatacaataa gtactaccaa tgtcagatat atataataat
      241 tatgtactac cattaaaatt aacttattta ataagatact actgtgttaa tctgaaaaaa
      301 attactttaa agtagttaca tactacctgt taaattagat actaccatta atacttaaat
      361 tagatactac ctatatttat attaagtact accgttctag gaataagata ataaaaaaaa
      421 actaccaaat tgcaattaaa tactacttaa aatgtaaaaa ataagaacta cccaaattag
      481 gtagttctta ataagagaat atcagataaa tttgtacaag tcataaaaaa attaatctta
      541 aatttttaaa tatatatacc tgttaattat aaactacttt atttaggtag tacataatta
      601 acagataaaa ctacatgctt gtagtttgtc tagctaatga gttgcatatg atttatataa
      661 aggtgtgcag cataacttag ttgaagttaa gagaatagaa taaatatttt ttgttttgct
      721 aataattaaa cactacctaa atatggtagt gtttaattat ataatattca ttttgttaca
      781 aataatcgta ttataaaagc ataacaattg ggataactat tttttagatt atttaagtac
      841 tacttaaatt aggtagtact taaattttgg tttaaggtag taggtaatta agaaataata
      901 tatatcttct tttataatag aaggggagag gcctagttaa aaacagcgac aattaataac
      961 aatgtattta atgcttcaca aaatccattt ggtggacaat gcattgttat tagcgatgat
     1021 ctaccgttct aagtagtttt ttctattggt tgcattcgtg actaattatg gtataattat
     1081 gagtaacaaa gaggtatttc attatatcat caaaccccat cagcgtgtag gctgatgggg
     1141 ttttttttac gtttctatat tatagaatta actggtaggc ttactgtctt ttttggcaat
     1201 tttgcaatca agccacttgc agatgtagtg tgcagttacc tctgcaaatg gcttgattgc
     1261 aaaaacaaaa tcaaaaaggt atttcattat atctcctcct ccccttgcca gattaggccg
     1321 gggtagcaga cagcaaaact attataacat aactgaaata cctaaatgac atatacagat
     1381 atgataattt atgtattgat ttttctaaca aaagcacata ttttaatata tactacctat
     1441 tttaggtagt atatattttt agatacaata tcgatcagtt ttgaggaaat gaaattctag
     1501 atattttttg ctacatagag ttactttcaa tattttttaa cgtaattagg cagaagtcac
     1561 tcatcctcta taggtttgtg atgaaattgc tattgatcca tttaagtaag agcaaaaaac
     1621 tggaggttta tcaggtaatt ggtctaatgg atttaagtat gcggtcaccg tgtatagagt
     1681 tgcttatgag attcaagaaa ataaagttat tccagtattg ctatatggaa cacataacaa
     1741 tttttatgaa ttagccgaga acactcgcaa ctttattcgt gtagatgaat cggcttcggt
     1801 tgagttataa ctttagctat atcaatcaac cgaccgacat atgttaaatc tatatataat
     1861 aaaaatacct acttaaatgg atgttaacgg aagtgtatgg tgagtagttc actcgtattt
     1921 tgatatcgtt tgtatatgtt ttcagcgttc taagtggctt attttgtcca ataataatat
     1981 ggagtggaat cccttaagaa acaccgttgg caattgtcgt tataacatgt tataatttat
     2041 ttatcaaaaa ctaaaagaag tgtgataaca tgggaaaaga actaaaggtt agaaagatcg
     2101 gcaatagcgt gggagtgatc cttccaagct ctcttggatt aaaaagcgga gacactatcc
     2161 aagccaaaca agaaggcaac ttattcatat tagatactac tcaaatagca aaagaacacg
     2221 atcgcaaatt gattgaagaa agtttccaag actttgagaa aggtttaaca gtttcagaaa
     2281 ttgaaatggt aaaagctttc ggaaaatacg gctggagtga atagatgaaa gagtacacgc
     2341 tagtttattc taaaagtttc cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg
     2401 agcttgctct ttctccagcc agcataaaaa gatttgtagg tgcgatccag aaggctttag
     2461 aactaaccaa aaattttcca actatgcatg aggaagtttc cgaaatctat ggaatggata
     2521 tcccaacata tcggatcgtg atcggcaaaa gctatgcctt attttatcgg atcgaccatg
     2581 aaaaaaaaga gatacttgta gg
//
LOCUS       DAZKAO010000203         2565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000203 DAZKAO010000000
VERSION     DAZKAO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.210"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1367
                     /locus_tag="ACG8H8_002747"
     CDS             <1..1367
                     /locus_tag="ACG8H8_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987755.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
     gene            1354..>2565
                     /locus_tag="ACG8H8_002748"
     CDS             1354..>2565
                     /locus_tag="ACG8H8_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP8987756.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVV"
BASE COUNT          857 a          384 c          433 g          891 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgta
//
LOCUS       DAZKAO010000204         2548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000204 DAZKAO010000000
VERSION     DAZKAO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.211"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            390..989
                     /locus_tag="ACG8H8_002749"
     CDS             390..989
                     /locus_tag="ACG8H8_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987757.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1038..1754
                     /locus_tag="ACG8H8_002750"
     CDS             1038..1754
                     /locus_tag="ACG8H8_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP8987758.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1768..2547
                     /locus_tag="ACG8H8_002751"
     CDS             1768..2547
                     /locus_tag="ACG8H8_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP8987759.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
BASE COUNT          922 a          378 c          492 g          756 t
ORIGIN      
        1 agagaaaata aatattccat atctacttta ttggatccat aacatgacta aattctgaat
       61 actttttaaa gcttatcatt tggtaaaact gaatataagt taattatata gactgaaaat
      121 tttgaatttt cagaatagaa taacttaaaa gtactgacaa attttttaaa ggggcaatat
      181 taatgttctg agataatcaa atcttaccga aaatatcaaa agtccaatgt gacacaattg
      241 gattttgtca cattcgttaa aatcgctcca tccttagagg ctctaatagg aaatcacttt
      301 tcttttagcg ccttttttct atagcttgtt taaatgtgac ttattatctg ttataatcgt
      361 attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt ggaaccgatt cgcgacaaaa
      421 aaaagatcga tgccatgaag gccattttgg cttccggaaa atatgggcaa cgaaatttgg
      481 tgcttttttc gatcgggatc aatacagcct atcgaatctc cgatttaaga cagttaaaat
      541 taagggatgt gctagaaatt tcccgtgggc gggtgatcgt caaagaacgt ctggccatga
      601 aggaacaaaa aacggccaaa cacaattcgg tcttcatttc gaacaaattg cgaaaagtga
      661 ttcttgatta tgttcaatcc gaatttcctg agcagttgca agcacaagac ttcagcaagt
      721 atttgtttcc aagtcgaaaa ggagcagata cgcctttgac ccgacaaagc ctttggcgga
      781 tcattcatga agcaggaact gcagttgggt taaaagaaat tggtccccat tcgatgcgta
      841 agacctttgg ctatttttta tacaagcaag gaaccaaaac agagatcatt cagtcgctgc
      901 taaaccattc ttcacaacgg gagaccttac gctatattgg aatcacccag gaagacaaag
      961 atactgcagt aaagagtttg gatttatgac agaataatgc cctatataat agaaggaaaa
     1021 attcaagagg tgagaaaatg aacgaagaaa agcagcctaa ctttcctgat aaatatcact
     1081 tatctcgcaa agagtctgtg tatttattga agaaaaacat tgtagaactg gtgtataatg
     1141 cggggaaatt tgaagggtta aatacaacgt tgttacaaac agaagggatt attaagtata
     1201 atcgggccaa caacgtggct gtagatgatg tcctgaccgt cgttaactta aaaagaggct
     1261 ttgaaatgct tttaaatgac gtacaggagc ccctgttaga gacaagtaag cggattaatc
     1321 ggatcgttgc agcagaggat gcgctttttc ccggtgaaat acgaaccggt ggggtagaat
     1381 tatcgacgat tcaaggaaga tatgtgccac caatgttaac tgaagacgag gtaaacaatc
     1441 aatatggtga gataatgaat caagagatat cagatacgga aaaagccctg cgactgtttc
     1501 tattcatttc gaaaaatcaa atattctgga atggtaatgt gggtacagcc ttgttgattg
     1561 cgaataagat tatgtttagc aacggtttag gactattatc aatacccgaa aatgtattta
     1621 tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa tctattagtc aatgagtcaa
     1681 atattatatc atttttatat gaacagtgta tctttggtat taattatttg aaaataatcc
     1741 aacatacaat ttaagaggta taagtaaatg atgactatag acgaagcaaa acaattagca
     1801 acttattttt cagttggttc agcgggatat attttagact ttactaatgt aacgtggaac
     1861 atatttacaa agaaaatcgt agggattgaa atacaaaatc attatggggg ctcaaaggga
     1921 gtatcctttc aaaagtttct gcttgacgat caatttgatg attcgttaaa gaatcggtta
     1981 atccaatctt taaaaggtat tatggatcga gataaaaaat atgaatctac tgaaatgaat
     2041 cccatagttc agttgctttt tgaggaagta tttaaagaag atacttcaac tgcattaaga
     2101 aaaatgaaat tttcaagcga gattgattta ctttatattc ggcagctacc cattaggatt
     2161 caaacagata ttaaaaacaa ggattttgac tctgttttga caaaagcaaa tactttaata
     2221 gatgaagttc taaaatttat tattgaaaat accgaaggac cgagtgttaa tattaattta
     2281 tttaagtcaa aagaattaag aaaagaagta tttggccgcc tcaatattaa aactgaaaaa
     2341 gatatggata atagaatcaa agcccttgtt ggagcactca atacattatc agataaaata
     2401 ctcgaaatga gaaatagtca aggagatgct catgcacatg gctcaaatag aatagtaatt
     2461 aaggaagaag aagcaattct tgttgcaaat tcttctatgg ttttgtgcga gtatgtgttt
     2521 actaagtata aaggattaaa tagataat
//
LOCUS       DAZKAO010000205         2512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000205 DAZKAO010000000
VERSION     DAZKAO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.212"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1139
                     /locus_tag="ACG8H8_002752"
                     /pseudo
     CDS             123..1139
                     /locus_tag="ACG8H8_002752"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            1419..2042
                     /locus_tag="ACG8H8_002753"
     CDS             1419..2042
                     /locus_tag="ACG8H8_002753"
                     /inference="COORDINATES: protein motif:HMM:NF026041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8987760.1"
                     /translation="MYHCTRKLLGLTDENLFFEEEWLETVEEDGFRTNLIHDKLSYIL
                     SHCRKCGIKNEGQIIKNGSHKTKVQLLPYRATKTELRLVRTRFYCKECQSTFNAQTNL
                     VDENCYLSKELKVQIALELAKNTIKKEIANRCFVSDVIVLRVLHTCLKTYHPRFDTLP
                     SVLCFDEYKSMKSCSGKNEFCFYEWADPAINRCIGESSPYFLKTIFP"
     gene            1966..2247
                     /locus_tag="ACG8H8_002754"
     CDS             1966..2247
                     /locus_tag="ACG8H8_002754"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987761.1"
                     /translation="MNGQTQQLIGVLENRRLTFLKPYFLNFTRKARANVKYVVMDMNA
                     PYFELVKAVFPNAKIVTDRFHIVQQITRTLNQLRIKTMNRFQKTEPTKY"
     gene            2251..2412
                     /locus_tag="ACG8H8_002755"
     CDS             2251..2412
                     /locus_tag="ACG8H8_002755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987762.1"
                     /translation="MKRFWKLLLTHAYDLDSSDYQYDRSFRRPMTQKAMVDELLSYDE
                     QLTRAYETC"
BASE COUNT          818 a          450 c          495 g          749 t
ORIGIN      
        1 cagcgacaat aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt
       61 cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca
      121 gaaccaaaaa tgctgaggtt cttgaagcga cccacgatat aaaaaccgtc gtcatggata
      181 agactggaac aattactgtt ggcaaaccac aagtgaccga tattatctct atcggaagaa
      241 ttagtgagaa cgagattctg cgaatcgctg ctggacttga ggattcatca gaacacccat
      301 tagctttagc agtaattaat gaagcaaagg acaagaaaat tactcctgcc gtagctaaaa
      361 atttcactgc tatttctggt aaaggggtac aagctttgat tgatggtaag caggctttta
      421 ttggtaatga tcgtttatcc gatgacttta acatgacaga tgatcttaag attaaaatga
      481 catctttaca ggcgcaggcg aaaactgtgg tattagttgg ttacgatggt caaataattg
      541 ctttaattgg gattcaagat gcacctaagt ccagctctaa agctgctatc agggcaatgc
      601 aaaaatcagg atttcacact gtgatgctaa ctggggacaa tcgtttggtc gcacaagcca
      661 tagcagatga tattgggatt gacgaggtca tagcagatgt tatgcctggg gacaaagcac
      721 aacatattag aaagttgcaa gaaaaaggag cagtcgcctt tgtaggtgat ggaatcaatg
      781 atgcccctgc attatccacg gcaacagtag gtattgctat gggatcgggg agtgatattg
      841 caattgaatc tggaggtatt gtactagtca aaaatgattt gatggatgtt gtaacttctt
      901 tagtattagc acgaaaaaca tatagtcgga ttttgattaa cttattttgg gctttcatat
      961 ataacgtgat aggtattcca gtcgcagcgg gtatattctc agcacttgga tttacactat
     1021 ctccagagtt agctggtctt gcaatggcac ttagctcaat tactgttgtt ttgagctcac
     1081 tattattgaa ctatgtgcgc ttgccaaaaa atagtgagac acttataggt aatagttagt
     1141 atttgtaata tacaaaagtg acgcttttaa ttataatagt aattttcctt tttcaaaatt
     1201 tgaatcagag tcttaaatac taggcataca ataagagaac caaaaatcag aactactgga
     1261 tgtagttctg atttttgatt ctacaatatt tagtgttggt ggaaaatcta cagattttct
     1321 actgacactt tttttgtaaa aaaagataga aaaagagata gcctaactgg tggaatttag
     1381 tcgtctaaaa caaaaccata ccaagaaagg atatctccat gtatcattgt actagaaaat
     1441 tgttaggttt aacagatgaa aatttattct tcgaagaaga atggttagag acagtagaag
     1501 aagatggttt tcgcaccaat cttatccatg ataaacttag ctatatacta agccattgcc
     1561 gtaaatgtgg catcaaaaat gaaggacaaa tcatcaagaa tggctcacat aaaacaaaag
     1621 tccaactttt gccttaccga gcaaccaaaa cagaattacg cttggtacgt acgcgctttt
     1681 actgtaaaga atgtcagtcg acattcaacg ctcagaccaa tttggtagat gaaaactgct
     1741 atctctcaaa ggaattaaag gtacaaattg ccttggaact agcgaagaat acaataaaaa
     1801 aagaaattgc caatcgctgt tttgtatcag acgtaatcgt tttgcgtgtc ctgcacacct
     1861 gtttaaaaac gtatcatcct cgttttgata cgttaccttc ggtcctttgt tttgatgagt
     1921 acaaatcgat gaagtcctgc agtggaaaaa atgagttttg tttttatgaa tgggcagacc
     1981 cagcaattaa tcggtgtatt ggagaatcgt cgccttactt tcttaaaacc atatttcctt
     2041 aatttcactc gaaaagcacg agcgaacgta aaatacgtcg tgatggatat gaatgcccct
     2101 tattttgaac ttgtaaaagc ggtcttccca aacgccaaaa tcgtgaccga tcgctttcac
     2161 attgtccaac aaatcactcg tacgttaaat cagttgcgaa tcaaaacaat gaacaggttt
     2221 caaaaaacgg aaccgacaaa gtattgacga ttgaagcgat tctggaaatt actccttacg
     2281 catgcctatg atctggatag ttctgattat caatatgatc gatcctttcg cagaccaatg
     2341 acgcaaaaag cgatggtcga tgagcttttg agttacgatg agcaattaac cagggcttac
     2401 gagacttgct agctcctcct ctatcacttt aaacacaagg acaaccaaag ttttttcgat
     2461 acgatcaaca gcttagatca atgccttcca caatggtttt gtaagaagct ga
//
LOCUS       DAZKAO010000206         2469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000206 DAZKAO010000000
VERSION     DAZKAO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.213"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..347
                     /locus_tag="ACG8H8_002756"
     CDS             <1..347
                     /locus_tag="ACG8H8_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987763.1"
                     /translation="NNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTPEEQIQRKLPE
                     FERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSKRFIF
                     NPKLEIYSVVIE"
     gene            672..1289
                     /locus_tag="ACG8H8_002757"
     CDS             672..1289
                     /locus_tag="ACG8H8_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987764.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1330..1629
                     /locus_tag="ACG8H8_002758"
     CDS             1330..1629
                     /locus_tag="ACG8H8_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP8987765.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1663..1830
                     /locus_tag="ACG8H8_002759"
     CDS             1663..1830
                     /locus_tag="ACG8H8_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8987766.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1875..>2469)
                     /locus_tag="ACG8H8_002760"
     CDS             complement(1875..>2469)
                     /locus_tag="ACG8H8_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987767.1"
                     /translation="GYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERPEFQK
                     LLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          850 a          381 c          465 g          773 t
ORIGIN      
        1 aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac
       61 ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga
      121 gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag
      181 taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg
      241 acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat
      301 ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa
      361 attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca
      421 taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt
      481 atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt
      541 atactttttg acatgccttg attttctttg tttatgtatg gcataataac cacataatac
      601 aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt
      661 agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg
      721 aacgacaact gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa
      781 aagccagcgg caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa
      841 aaggagattt actctatcta caatcattag atagactagg cagaaataaa caaatgattt
      901 tagaagaatg gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc
      961 ctctgttaga tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact
     1021 taattctgca attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc
     1081 aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg
     1141 ctttacctcc tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag
     1201 tctatgctat ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat
     1261 atgaacatac cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag
     1321 agtttgaaaa tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg
     1381 acacgaaccc aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt
     1441 acttcaaaaa caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct
     1501 ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc
     1561 attttatcag aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg
     1621 tatttataat cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat
     1681 gatcgttggg gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt
     1741 gactgtgggg aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc
     1801 catcgccgat acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg
     1861 aatgtagtat atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag
     1921 atcccgtttt cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg
     1981 gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt
     2041 cccttatatc ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt
     2101 ttctcacgct cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg
     2161 gtatccagga gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac
     2221 gctacttttt tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct
     2281 aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac ggatataatt tagtaatttt
     2341 tgaaactcag gacgctcagt atctttacca ctggcttttt cttggaataa tttatcaatg
     2401 ttaagtcctt ccatcattgc caattgtcga tccaaatttt gatcttgtga agatactcga
     2461 atatagcca
//
LOCUS       DAZKAO010000207         2460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000207 DAZKAO010000000
VERSION     DAZKAO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.214"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1158
                     /gene="gap"
                     /locus_tag="ACG8H8_002761"
     CDS             148..1158
                     /gene="gap"
                     /locus_tag="ACG8H8_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP8987768.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(1243..2178)
                     /locus_tag="ACG8H8_002762"
     CDS             complement(1243..2178)
                     /locus_tag="ACG8H8_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8987769.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            2364..>2460
                     /locus_tag="ACG8H8_002763"
     CDS             2364..>2460
                     /locus_tag="ACG8H8_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987770.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMG"
BASE COUNT          751 a          510 c          439 g          760 t
ORIGIN      
        1 tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt gacaaaatat
       61 tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc gtttcacagc
      121 acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa cggatttggg
      181 cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat tgaagtagta
      241 gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt tgactctact
      301 catggtactt atcctggcac agttactgca acagaagact caatcgtagt ggatggcgaa
      361 gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa agaaaacggt
      421 gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc aaaagcgcat
      481 ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat gaaaaccatc
      541 gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc agcaggttca
      601 tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt tgggatcgaa
      661 gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt agatggacct
      721 gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat cccacattct
      781 actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa attacaagga
      841 cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc agtattgaaa
      901 acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct
      961 tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac aactcaaggt
     1021 tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca acttgttaaa
     1081 actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg cttactagaa
     1141 aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg actggatcca
     1201 gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa catattggct
     1261 taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg cttccgcatg
     1321 ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat agaccttatc
     1381 taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca ttgggcgctg
     1441 gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag cacggacttt
     1501 tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca tatcgtctag
     1561 ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga ttgctttcac
     1621 attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca tgctgacacc
     1681 gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta tccactgtac
     1741 atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt ttccatgtcc
     1801 tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg cgtaattggt
     1861 catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt cacttttcgt
     1921 ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga tggtccaatc
     1981 gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg atgtctgtgt
     2041 tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag cataattata
     2101 aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc ctaaaaggat
     2161 tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat aaccttgaat
     2221 tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc gaattgtaaa
     2281 attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt ccgacgaaag
     2341 aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac gaactgacaa
     2401 tgatagaatc atattatctt caacataata agccggttga aatcgctaac cgaatgggac
//
LOCUS       DAZKAO010000208         2454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000208 DAZKAO010000000
VERSION     DAZKAO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.215"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..1567)
                     /locus_tag="ACG8H8_002764"
                     /pseudo
     CDS             complement(186..1567)
                     /locus_tag="ACG8H8_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2065..>2454)
                     /locus_tag="ACG8H8_002765"
     CDS             complement(2065..>2454)
                     /locus_tag="ACG8H8_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP8987771.1"
                     /translation="IKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAI
                     SNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHI
                     PRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          704 a          422 c          430 g          898 t
ORIGIN      
        1 gaaaatctag tcataaccag ttaggcagag tgcttaactg gtttttgttt tacttggaag
       61 atcaagcctt gaatgagata aattcttgct cgaaagttat ggaaatttcg atagccgtag
      121 gccacttgta ttgtgcaata gttttttaca aaaaaaaggg ggtgcggata tttttttgga
      181 cctttttaac aaatatatct ttgcttcttc cgatattgaa gagggcttag ccctcctaaa
      241 gataacttaa ttcgttgatt attataccaa tgaatataat catccaattt gtttataaac
      301 tgattgattg ttgtattctt ccagtcacgt tgataaaaca tctcgttttt taatcgtcca
      361 aagaaacctt cgcacgctga attatctggt gaataaccct ttttagacat agagtgaagc
      421 aagttggctt tgttccaata gtccaactaa caatagcacc atcgtaacaa tcgacaagtg
      481 gtgataagta gattttccct gcgggaattc taaattcagt aatatctgtt aaccattttt
      541 catttggctt ctttgcttta aaattccgat ttaataaatt ggggtgtgca ggagaaattt
      601 ccccagcata cgaagaatac ttttttcgtc tagaaaattt aacaaataga ttttcctcag
      661 tcatcaatct acgtacaact ttttcagaaa gctttttacc catatttttt aaagcttggt
      721 gaattctacg gtaaccatac gcacagaagt tatgattgaa aatcgtgata attaattgac
      781 gttctttgta atacttatct ggtttctcaa gtgcttgttt ttgataaaaa tagctgcttt
      841 taggcaactg gagctgttga agaagctctt tcaaggtgaa ctgatttcgt aaggcatcaa
      901 tcaatatcgt tttttcttga tttgatagac aaagaagatt gacgcctttt tcttttttta
      961 gcagttcagc cgctccttcg agtaatgctt tttcgagctg taattggtgt acttgttttt
     1021 gtaagtcttc tacttccttt aaaagtagct ctttttgttt ttcctgattt tttaaagtca
     1081 tacgcaatgg aaaatccttt ccaagaaccg tgtttttcca ttgatataga gtaactctac
     1141 taattccttc agattcggct aaatttttcc tattagtttg cggtttatag aattttttca
     1201 acacagcttc tttttgatct tgtgttaact ttaccgcact ttttcggagt tttctagctc
     1261 ctggtgcaag ttcttcacac cactgccgca aggactctct attaggataa ccaagcatgc
     1321 gaatggttcg tgcataacat tgaccatatt caaaataatg attcaccgct attcgttttt
     1381 gttcttcaga atactttgat ttttttcggt gctcctgatg gagctctccg gattccaaat
     1441 actctttata ccatttccgt agtgcaccaa tcgacggata ccctaattct cgaatggttg
     1501 cagcataaga gcaatcgtat tttaaaaata actgaattgc ccttacacgc tctttgtggg
     1561 aaaacacacg gacattcctc ctaagtttat tttttcgtcc aggattatgt ccgcaccccc
     1621 ttgctttctg atgacttatt ctggttcttt gagtgtcaat gcatagatgt gatgtagcta
     1681 gttagaaaaa aattttgaat actctttttt tctttcagac tcctttttaa gtaagatttt
     1741 gaagtgtgag ttacggggtc agaaacttaa aagtttttgt tcctttattt tcaagtctga
     1801 tttggcagct tttcggcagc tcatttattt atggtcagaa atagatgctg aaattttaat
     1861 ctattagcaa atagtccaga acgtatgaag aggagtcgga tatcatttgt cattaacacc
     1921 ataaatattt ataaaattcg tgttcaccta caattaaatt taatatataa tattcgttgt
     1981 aagggcttat aattttttgt taatgaacaa gataggaaga aacatttaat ggacagtttt
     2041 cagagattgt cactatatga tttgtcaaat taagctagaa taaaatgctt cttgtacata
     2101 gctcaaaaat atttcgattg gtgttctgta attcaatgat tttcttggaa tatgattacg
     2161 ttgattgctg acactggaaa taaatgtctg attcacttct ctaaaatcca ttgatttcgg
     2221 cagtccatta cgacgcagaa tcccgttaga attctcgttt aatgggcgtt gagaaggtgt
     2281 tccagggtcc gcaaaatata tatcaatatc atgttggtta ctaatggctt tccagttaga
     2341 aaattctttt ccacagtcaa acgtaataga tttaaagaag tttttaggga agcgagaaaa
     2401 ccattgatta agggcagttt caatatctaa tgccttacgg ccgttgggtt taat
//
LOCUS       DAZKAO010000209         2390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000209 DAZKAO010000000
VERSION     DAZKAO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.217"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8H8_002766"
     CDS             197..2059
                     /locus_tag="ACG8H8_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8987772.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACG8H8_002767"
     CDS             2059..>2390
                     /locus_tag="ACG8H8_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8987773.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKAO010000210         2337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000210 DAZKAO010000000
VERSION     DAZKAO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.218"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..1640
                     /locus_tag="ACG8H8_002768"
     CDS             468..1640
                     /locus_tag="ACG8H8_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFP8987774.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1770..>2337)
                     /locus_tag="ACG8H8_002769"
     CDS             complement(1770..>2337)
                     /locus_tag="ACG8H8_002769"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFP8987775.1"
                     /translation="EEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILL
                     RETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIID
                     FGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIE
                     TIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          810 a          433 c          333 g          761 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg aaattcccac aatcgactaa agagtaccaa tctaattgaa cgactgaatc
     1501 aagaagtacg cagaagagaa aagattattc gcatcttccc caatcaaaca tcagccaatc
     1561 gcttaattgg agccgttctt atggacctac atgatgaatg gatttattct tcaagaaaat
     1621 acatcaattt tgataagtag aaatggtaaa aacattgtat agcattttac acaggagtct
     1681 ggacttgact cacttccttt attatttttc atttttttaa actatttaat actaatgtct
     1741 tttataatag cttttcatat attatataat caatctttat aagtcctttt ataaatttct
     1801 tttctaccat tttcgataaa ttcctgttta atatttttaa ttccataaac aatagtttca
     1861 ataggataat attcttcaac tatatcttga tattcttttg ctttctcaat atctatattt
     1921 ccatacattc ttaatatatc ttctccaaaa tttgttccta tttcttcttc actatcttca
     1981 agtaagtata taaaatcaca atattcatct ataattccag aatctccaaa atcaattatt
     2041 ccagttaatc tattattgcc atctaacaat agatgattac aactaaaatc attatggcat
     2101 aaacactttt taccctcaaa aactgttgtt gcatttagtc tttccataaa actttctata
     2161 taatcttttt ctatatcagt taaatcatta taaatagttt cacgcaacaa tatatactct
     2221 tctaatacat tttgtttatt atcaatagta cattcactaa tatctgtata atctaaaccg
     2281 tgcatttgtc ttaaaaaact ggcaatatct cgttttaaca aattttgttc ttcttct
//
LOCUS       DAZKAO010000211         2337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000211 DAZKAO010000000
VERSION     DAZKAO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.219"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACG8H8_002770"
     CDS             <1..1192
                     /locus_tag="ACG8H8_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP8987776.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1326..>2337
                     /locus_tag="ACG8H8_002771"
     CDS             1326..>2337
                     /locus_tag="ACG8H8_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8987777.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNF"
BASE COUNT          919 a          315 c          460 g          643 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaatta
     1441 gttacttaga atgtgggaca tcgttgttca taaaaagaga agttagcaat ttagaagatc
     1501 ttttttatca aaatagaaga gaactaaatt ctaagataaa aaagttgttt ttggagacag
     1561 cagttgacaa aattgatatt caaaataaaa agattatttg tgtaaaaaaa gatggcacaa
     1621 gattttccca agaatatgac aaacttatac tagctacagg ttcaaagcaa attatgttag
     1681 atgtaccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgctaaag
     1741 agatttttaa agctttagat gataatacta ttaaaaaagt aggaattatt ggagcaggat
     1801 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     1861 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     1921 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     1981 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2041 tcgtacaagc tataggattt ctatctaata gtccaattgg caaaaaggaa ctcttgagag
     2101 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2161 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2221 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattgac aatcaagatt
     2281 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aattttt
//
LOCUS       DAZKAO010000212         2336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000212 DAZKAO010000000
VERSION     DAZKAO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.220"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..163)
                     /locus_tag="ACG8H8_002772"
     CDS             complement(<1..163)
                     /locus_tag="ACG8H8_002772"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8987778.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
     gene            complement(498..728)
                     /locus_tag="ACG8H8_002773"
     CDS             complement(498..728)
                     /locus_tag="ACG8H8_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP8987779.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(743..1693)
                     /locus_tag="ACG8H8_002774"
     CDS             complement(743..1693)
                     /locus_tag="ACG8H8_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP8987780.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1690..>2336)
                     /locus_tag="ACG8H8_002775"
     CDS             complement(1690..>2336)
                     /locus_tag="ACG8H8_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8987781.1"
                     /translation="FIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSSFLALGVIL
                     IGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLITSFDPLMAK
                     AMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFIS
                     ATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT          757 a          437 c          295 g          847 t
ORIGIN      
        1 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
       61 gtttataaaa agcacccgta gttacgcctg cggtcaagca cagttgcctt atcttagttt
      121 gctgaaatcc tactttttca aaatggctta gtccactttc tattaatttt tggtgagttg
      181 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actatattat
      241 cttacacagg acttgaagtt tcaatcgtta acgtctatta ctgaactcat tccttagagc
      301 taaactaatg agaaagagca aaataactga tacaaaaagc aatcctacaa gaattaatag
      361 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
      421 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
      481 aaaataaagc cttttactta aaaccttttt ttgtaatcgt caataaattt tttcccttta
      541 tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagt cagcatctca
      601 cttacagatg ctggactaac acttagaact tttgctaaat cattattttt taatttcttt
      661 tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt tgttttaatt
      721 tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc
      781 atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa
      841 aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt ggtctatcgt
      901 ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt
      961 gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag
     1021 gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt
     1081 tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt
     1141 caatatactt atctagattt tctttataga attttttatt atcaggatct ttttccgcta
     1201 actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac caagcatggg
     1261 gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg
     1321 ctgcaaaata gtctttgttt tcctctttgt tcgcattttt tactaatttt gtaaaccaag
     1381 catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt
     1441 tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac
     1501 tgtgtaaatc aattttatct tttgctatat tttcagtaat atctgctaaa atcgaattag
     1561 taactactac ctttaatttt tcgttcgaag ccactgtatc tttgtcttta ctatttgttg
     1621 aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc
     1681 attttctcat cataactttc tctccttctt tcttaaaaat ttttgttttg gtgagattag
     1741 aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt
     1801 aaaactataa ccaatgaaca aacctagcaa tgatgagcac gctccgactg tcgccgaaat
     1861 aaatatcata gtttttaaac tgtttgcata taaatatgct gtagcagctg gtgtaattaa
     1921 cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa
     1981 tagaatcatt aatagataat gataaaaact gacattcatc cccattgctt ttgccattaa
     2041 tggatcaaaa gaagtgatta ataattcttt aaaaaataga acaactgtaa ccaacactaa
     2101 aacagaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
     2161 taagatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctaatgc
     2221 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
     2281 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaa
//
LOCUS       DAZKAO010000213         2331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000213 DAZKAO010000000
VERSION     DAZKAO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.221"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..>2331
                     /gene="esp"
                     /locus_tag="ACG8H8_002776"
     CDS             63..>2331
                     /gene="esp"
                     /locus_tag="ACG8H8_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP8987782.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          841 a          355 c          474 g          661 t
ORIGIN      
        1 cgtatcagat ttatctttta ttcttactaa gaaaataact attaagggag ttgatttgaa
       61 taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa cgagtattga
      121 agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttggttggt gttggtcttg
      181 tcttgggaac aactgaacta gttcaagcac aagatgaaat ttctccaagc accccacttg
      241 aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat acttttcagg
      301 agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt cctcaagaac
      361 taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga aaacataata
      421 ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga ttagctaaat
      481 atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga cggacctttg
      541 taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat tatattcgtg
      601 caagtgatgg attatttgct ggggctgaaa caactgatac acaaactgct cctaatgggg
      661 taattacgtt tgaaaagagc ttagatgaaa tttttaaaga gataggcata gacaatctca
      721 agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat gataaaattg
      781 taccaggtat tgatagtacg ggctactttt taacagattc agatgatttc tataatagct
      841 tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg
      901 aggaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat ggagctataa
      961 tcttagatca taagttaacg aaaaattata atttctcata ttcagcttcc gcaaagggca
     1021 cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta gcgggtatac
     1081 agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcttttgaa tcagggaaaa
     1141 aagtggctga tttagcaatt gaacggcgca aagatcatga aaattatggt gtaggttcaa
     1201 ttactgataa tgatcttacc aaacttattg attttgctaa tgcaagtcca cgtccagtcg
     1261 ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa gatatgaaag
     1321 caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt gattcttggt
     1381 tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat tattatttgc
     1441 aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac acgaatccat
     1501 atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt aaggggaaag
     1561 tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat aaagaaggta
     1621 ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca atttcagata
     1681 ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa
     1741 aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc gttattaagg
     1801 aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca gatgctaaag
     1861 aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg aaaacagagc
     1921 ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag atcggaaatc
     1981 gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg gaaaatgatg
     2041 caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga aaacctacat
     2101 ttgataaacc agatgttcct ttagctaaag atgccttttc aattttagat gtctataata
     2161 aggacttcgg taatgcaagt gttgatgcaa atactggtat tgttacattc actccagcta
     2221 aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt gtttaccaag
     2281 atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt t
//
LOCUS       DAZKAO010000214         2316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000214 DAZKAO010000000
VERSION     DAZKAO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.222"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG8H8_002777"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG8H8_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG8H8_002778"
     CDS             612..1124
                     /locus_tag="ACG8H8_002778"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987783.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG8H8_002779"
     CDS             1121..2158
                     /locus_tag="ACG8H8_002779"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8987784.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAZKAO010000215         2284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000215 DAZKAO010000000
VERSION     DAZKAO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.223"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2137
                     /locus_tag="ACG8H8_002780"
     CDS             <1..2137
                     /locus_tag="ACG8H8_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP8987785.1"
                     /translation="KKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLF
                     VALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITY
                     TRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEK
                     QLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELA
                     VVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRN
                     DRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQS
                     KVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALG
                     TITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALD
                     AVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTG
                     IDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVA
                     NGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHG
                     TDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVL
                     PNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          781 a          413 c          508 g          582 t
ORIGIN      
        1 gaaaaaaata ttaaatactt cttggttaga aaaaataaga aaaggtgcac aagaacctaa
       61 gaacaaaacg tttcgccggt cccatgttcc ttttcgctta aattttcttt ttttcattat
      121 atttacgtta tttgttgcgt tgattgctag attgggttat ttgcagatcg tgaacggcga
      181 agagatggag gcgcgagtga agtctacttc tacgattacg gtccaagaaa gtactcctcg
      241 cggaatgatc tacgactcca caggaaaagc tttagttacg aataaagcga atcaggccat
      301 cacatatacg agaggaacac aagttacggc taaagatttg cttgaaatcg caacgaagct
      361 gaactcgctg atcgatgttc cggtagatga aaatctgaca gatagagata aaaaagattt
      421 ttggctggct gatcccaaac atttgaaaga agccacaaaa agactttcgg caaaagaaaa
      481 gcaattaggg aacagcgaac aatatagtgc gatggtcaat aaagtcacag ctgatgagat
      541 taatttaaac gaggagcaat taaaaattgc taccatcttt aaacggatga atagtgcata
      601 tgaattaaac actgtctata tcaaaaactc tggtgttact gatcaagagt tagctgttgt
      661 ggcagaacgc gcttctgaac ttccaggagt ttcgactggc acagactgga cacgagaata
      721 taatgcagca agttcactga aaagtattct tggcagtgtg acgactgaaa agcaaggact
      781 tccagcagat gaagcagaga aatacctagc aaaaggttat tctcggaatg accgtgtagg
      841 acaaagttat ttggaaaaac aatatgaaga tgtcctgcaa ggaaccaaaa cccaatatga
      901 agtttccctt gataatgaag gaaatgtttc gaatcaaaaa gaaatcttct ctggggaaaa
      961 aggttctaat ttaatgttat ctatgaatgc agaattccaa tcaaaagtag aagaaatttt
     1021 gaaaaggaat tatcaaacat tgatcaacaa cggcaaagca cagtattcgc ctggtgctta
     1081 tgcagtggct atgaatcctc agacaggaga agtgctagca atgactggtt tcagtcatga
     1141 acaaggttcc aaagaaatca ccgagaatgc tttaggcacg attactagcg cctttgatcc
     1201 agggtctgta gtcaaggctg gtactttgac agcaggctgg gcaagtaacg cgatcagcgg
     1261 aaatcaagtt ttgattgatg agccgattcg tctgcaaggt gcttcagaaa aaagctctgt
     1321 ctttaatcga agcggacaag ttgctcttga tgcagtcaaa gcatcggaat tgtcttctaa
     1381 tacgtatatg atcaaagtgg cgttaaagat gttaggacta gactatacac ctggaatggg
     1441 actacctagt ttagatgaag aagctaaagc ttatcagcaa ttgagagact ctttcaaaga
     1501 gtttggtctt ggtacaacga ctgggatcga tttgccgaat gaatctcctg ggatctcacg
     1561 ttctgttgat tatatgaaga agttcaatgc ggataatggc aaagaatggt atgctccagg
     1621 taactttacc gatttggcat ttggacagtt tgacacatat acaccaatcc aattagctca
     1681 atatgcttca acagtcgcta atggcgggaa acgagtacag ccgcacctag tcaaagcgat
     1741 ctatggcaat gacgaaaatg gaaacttggg tgaagtgaag aaagaaattg gcacaacagt
     1801 agaaaatacg gtcaatattt ctgcagaaaa tatggcgatc ctccgtgaag gattccacca
     1861 agttgtccat ggtaccgatc cttacacaac agccaaacca ttagccagtg cgaaaatgga
     1921 cctttccgca aaaacaggga ctgcagaaac ggttgcggaa ggtcatccag atattgcgac
     1981 agtcaatagt aatatcgtgg catacggacc aacagacaat ccggaaattg ccatcagtgt
     2041 tgtcttgccg aacttattag acgaacaaga ccacataaac ttgacgattg ctaaagaaat
     2101 aatggacgcg tattatgata tgttcatggc aaaataaaaa agaggtcgtg aaaaagccgc
     2161 ttagtttcga gtatgaagaa aaattttgtg aaaatagctt ctcacatttt tgcttaaatt
     2221 tgacttcata ccgagaaact ggcttttgaa caccgtttac tcggcaaaaa aggttgtgac
     2281 aaaa
//
LOCUS       DAZKAO010000216         2256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000216 DAZKAO010000000
VERSION     DAZKAO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.224"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG8H8_002781"
     CDS             43..165
                     /locus_tag="ACG8H8_002781"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987786.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACG8H8_002782"
     CDS             138..269
                     /locus_tag="ACG8H8_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987787.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            808..1008
                     /locus_tag="ACG8H8_002783"
                     /pseudo
     CDS             808..1008
                     /locus_tag="ACG8H8_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1369..1674
                     /locus_tag="ACG8H8_002784"
     CDS             1369..1674
                     /locus_tag="ACG8H8_002784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987788.1"
                     /translation="MVHYRKILELYFSGISQRTISSTVGSSRHTIREVTSRADRKGVT
                     ELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKNMTLKLLHREYGVHGT"
     gene            complement(1720..>2256)
                     /locus_tag="ACG8H8_002785"
     CDS             complement(1720..>2256)
                     /locus_tag="ACG8H8_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987789.1"
                     /translation="EDLNIDKLFQEKASGKDIQRPEFQKLLNYIREGDCVIVASLDRL
                     GRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNER
                     EKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKRLVFKTVVDMLNQGVPVAEIAK
                     ENGLSRPTIYKIKKANDL"
BASE COUNT          742 a          375 c          414 g          725 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catttaaaaa gaaaagtgac
      361 agataggaat aaaaacttat taaatgtagt tatgatctgt tatatttcat ttctaatact
      421 aggtcttcca caagcagtgt ttcctgaaat gttttctgga gactggggta ttgtcagcta
      481 ttctatatta acattgattg ctatggtttt aggtacttca ttttataatg cttttgtaag
      541 taagcatgtt gaggaatatg aagtattaat gtacttaaaa gagaaaaaaa caagtaatta
      601 atatgagaac accacgcata gtttttattt tgagatatcc tagaatgaaa cacattaaga
      661 acgaaagcaa agcaaaagac gccagaactt ctactacctt ctaagttttt tcatatttgt
      721 ttgttatgct atacatagat ctgcataatt agacttgcgc tgcccgttat tgaagtttca
      781 ggcagcgctc tttttggggg atcgataatg gaagaagaaa aaatatttga gaaacgctgg
      841 cagctagcat caagtgaaca aagagcaaga tacaataatt tgatcacctc ctaccccact
      901 gttgactaga catataagga aaagaaatac ttactttggc tgtgccagtt agatatagat
      961 acctttgaaa catttgaagt gatcctagat aaaattaaac agaattgatc agaaacaagc
     1021 gaagccttct ctcggttcgc ttgttttgct atttattagt tggctcaggg caactctaag
     1081 ctcgtttgaa agaaaaccga tggataatcc ccttgtttgt accactttag aatacatgga
     1141 tgtacgtatt gccattgact cctattagtt actgaaaaaa aatttaaaca agttgagtat
     1201 tgcagataaa tagctccagt gaggtgaata actgctccac tcaaggcaat agatagctcc
     1261 atccagggca acaaactgct ccactttcat atttaataga tgaataacct tctgtataat
     1321 agaaaggcat acctttcggt atgacaactt atacaggatg ttattttatt ggttcactat
     1381 cgaaaaattt tggagcttta tttttccggt atttctcaac gaacgattag ttcaactgtg
     1441 ggaagctcca gacatacgat tcgagaagtt acttctcgag cagacaggaa gggggtgact
     1501 gaactaaccg ctgatatgag cgatacttgg ctgcaggaat atctttttcc ggaaaaacaa
     1561 acaacagcac gaggttacct tcctgtagac tgggaatatg ttcataaaga actaatgaaa
     1621 aagaatatga cattgaaact ccttcataga gaatatgggg tacatggaac atagaaacga
     1681 aaatgcacgg tagaaaagcg aatgaatgta gtataaattc tacaaatcat tcgctttctt
     1741 tattttatat atggttggtc gagagagacc attttctttg gcaatttctg caactggaac
     1801 tccttgattt aacatgtcca ccacagtttt aaagactaat cgtttttgcg gatctgttga
     1861 gttctcagaa tattcaattg gtcgcccctt atatcgacca gcttttttgg ccaataatac
     1921 tccttgccgc tgacgttcac ggattttctc acgttcgttt tgtgcaatat aacttagcaa
     1981 tgataaaaac atatcgaaca tagcagtatc taggagttca tttccagtat taaaatctaa
     2041 aaactgtgca tctagtattt ttaatgctac ttttttctgt ttcataaagg taactgtatt
     2101 ttttatgtct tcataatctc tgcctaggcg atccagtgat gcaacaatga cacaatcacc
     2161 ttctcggata taatttagta atttttgaaa ctcaggtcgc tggatatctt taccactggc
     2221 tttttcttgg aataatttat caatgttaag gtcttc
//
LOCUS       DAZKAO010000217         2232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000217 DAZKAO010000000
VERSION     DAZKAO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.225"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1022
                     /gene="msrA"
                     /locus_tag="ACG8H8_002786"
     CDS             189..1022
                     /gene="msrA"
                     /locus_tag="ACG8H8_002786"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8987790.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1102..2091
                     /locus_tag="ACG8H8_002787"
     CDS             1102..2091
                     /locus_tag="ACG8H8_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8987791.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          814 a          366 c          444 g          608 t
ORIGIN      
        1 tccataaaag gtaaattaac ttttcattta taaaataaat tgattaaaaa ggagaatctg
       61 tcgataagga ttctctcttt tttatacgaa aaatctgtag ggggatgaag taatcgctta
      121 tttcctcact ttatgataaa ttacagctaa agtcactagc ttgtcttctt cggaaaggag
      181 gagaatctat ggatgaggaa caaatattca ttcaaacact ttataatctg attttgaatc
      241 cgaatacacg agattgggaa cgaaaagtat tgattcagac aaagaatgac ataagagaaa
      301 atatatccgt taaagaacag ttatcaaaac tagaagcaac actacgtcca ttagctatcc
      361 gaatgaatct gacgcctgat gtaatggact tttatttatt gattacggaa ggatttgata
      421 aggagcaaaa atatgacttt tccaagcatg ccatgcaaga tgctgactat caagaacgtg
      481 cagtatttgc agggggatgt ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga
      541 tcctatctgt tttgtcagga tacacgggcg gacatgttga aaaaccaaat tatgatcaag
      601 taagtggtgg ttacacaggt catgtggaag ctgtggagat catttatgac acaagagaaa
      661 taagttatag cgagttgctg acaatctatt ggcaaattac tgatcccaca gatacatttg
      721 gacaatttca agatagaggg aagcaatatc gtccagtgat tttttatcaa ggcgaacgac
      781 aaaaagaatt agcggaacaa tcaaaacaga aactagacag ttcaggaact ttccaccaac
      841 caatcgtcac aaaaatcgaa ccagccggta cgttttggcc agcagaaaac tatcatcagc
      901 aattttacaa aaaacaaccg aaacgttata aaaaaatcca gcaagcacgt aatcaatttc
      961 tgatctatca acgagtaaaa aacaaatggc aaaaaaatat acgaaaaaat cattttgatt
     1021 aactcctttt ctttaaaatg tagtaaacta aaaatagaaa aaagaaagcg ttttaaaaag
     1081 atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga aaaaatggaa
     1141 tatcgtaaat ccggtcattc aggtttgaaa ttaccgattt tatctctcgg attatggcaa
     1201 aattttggtg attatgatcc tatccataat caaagagaga ttttaagagg cgcattcgac
     1261 atgggaatta cccatttcga ccttgcgaat aattatggag gccctgctgg agctgcagag
     1321 aaaaactttg ggcgtatttt tagagaagat tttcaagcct accgtgatga actgattatt
     1381 tctagcaaag caggttatca tatgtgggaa gggccatatg gggaatgggg ctctcgtaaa
     1441 tccatcattt caagttgtga ccaaagtctc caaagaatgg ggctagatta tgttgacatc
     1501 ttctatcatc atcgcccaga ccctgataca cctttggaag aaacagcaga agctttgatg
     1561 caacttgtaa gacaaggaaa agcattatat attggtatat ccaactataa tggagaagat
     1621 acaaaaaaaa tgactgaaat tttaaaaaga aaggaagcac cgttcattat tcatcagatg
     1681 cgctacaata tgttttctcg tgcactacta gaagacgatc tatctccagt attagaagaa
     1741 gaagggctag gggcaatcac gttcagtccg cttgctcaag ggttgctgac aaatcgttat
     1801 ctacatggta ttcctgaaga ttctagagca catcgtaagg aaataccatt cctttcagaa
     1861 gaacaagttg gatcaactct ggaaaaaatc aaagcactgc aaacaatcgc cgtatcacga
     1921 ggacaatcat tagcgcaaat ggcccttgca tggaatttgc gacaaaaatc tgttacaagt
     1981 gtactggtcg gagcaagtcg gttaagtcag ttacaagaaa gtgttcgaat gatggataat
     2041 ttagatttct caccagaaga agaattgaga atcgaccaaa ttctagaata gaaacgatgt
     2101 aatcaaactt tttatccata tattaaaaaa aacaaaagat gacatcaaga atcgaatgaa
     2161 gatcgtctgt tattgaacat catatttttt gtcgttcaaa ataaaaatga tcgaaggcta
     2221 ggagtgaatt tt
//
LOCUS       DAZKAO010000218         2229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000218 DAZKAO010000000
VERSION     DAZKAO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.226"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..408)
                     /locus_tag="ACG8H8_002788"
     CDS             complement(58..408)
                     /locus_tag="ACG8H8_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987792.1"
                     /translation="MIDYNDRGRLKWTGFYLSDHTEEIDADNEQRANQNLAKEQITTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPYDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(401..1726)
                     /locus_tag="ACG8H8_002789"
                     /pseudo
     CDS             complement(401..1726)
                     /locus_tag="ACG8H8_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(2030..>2229)
                     /locus_tag="ACG8H8_002790"
     CDS             complement(2030..>2229)
                     /locus_tag="ACG8H8_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987793.1"
                     /translation="YLSDLSLESKYAENESMKEVFKRLKILYGMNNSENPNKDVSFLS
                     NFSILFELLNDFPQLKRELEQ"
BASE COUNT          648 a          442 c          339 g          800 t
ORIGIN      
        1 aatatttgta tataactata catgttatat actttagctc atattgctgg ataatgttca
       61 ttctagtcga ctccacttat tatcctggta aaattcaatg tgccgtattt cgtcgtagtg
      121 aattttgtct tttccaacgt agattcccag ctcatcacta cctaagatct ttccaacaat
      181 atcgtatgga tagtatcctt cagcatttcg ttcttctttt tgaatacata ctaatctatt
      241 ttttatgact gcagcgttca atacttccga aatctcttca gtcgttattt gttcttttgc
      301 taagttttga ttggctcgct gttcattatc tgcatcgatc tcttccgtgt gatcggataa
      361 atagaatcct gtccatttca atcgtcctcg atcgttatag tcaatcattt ttaagccccc
      421 ctgcaccgcc tgcatggcca ccaaccaacg tgcttctagt aatactacga gcgccttcta
      481 acatcgaaga agcatgtaca attgacgtaa aaccatactt ctgcctaatt ttatctatta
      541 ttttgtctag tttttcgtca tcaatctgtt tttgtggatc actaaacaag tccagctgaa
      601 gcgaatcggt gtacacaagc ttgctatagg ttattccaac gtgccttact tcttgtcctt
      661 cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta ttatctgtga
      721 cagggatctt catctggtgg aaaaatcctg gtctggtctc taagatcgac gtaccaatgt
      781 ttaaatggat acatcctgtt ttacaattat gctgtctgat acgaatggct acttgttccg
      841 ccatttcttt tatcaccagc tcgatttcat ttcttctagt ataatctcgt ggtaacactt
      901 gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gactgctatg tctgttcgat
      961 cgattccatt tgcatggaag tacagctgta agccaatcac acctaaacga ctcttaatag
     1021 tgtgtggatt ccaattagct aagtctttaa tactcatgat acccatttag ttgagccttt
     1081 ttttcattcg agagccaatg ccccaaagat ccgtcatttc aggaatattc catacttttt
     1141 cgggaacgtt ttcgtacgtc cattctgcaa caaatccttc attatgcttg gcttcattgt
     1201 ctaaggctag tttagccaaa aggggattat ctccgatacc taccgtagca atcaatccta
     1261 gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt tgacttcgtg
     1321 ttccttcagt cgtaaaaagg ttcagtgatc tggtcacttt taagatcgat tcatcgatcg
     1381 agtaaatcaa taaatcttcg tctgctacat atcttctgaa gatattgttt acctgcatgt
     1441 ttcgcttaat atagagattc attcgtggtg gaacgacatg taacgtttta ggaaatggtt
     1501 gtggtaagtc acgtggtcta ctgacattag aaataccata cttcttcttt gcagctggtg
     1561 aagaagctaa aatcaaccct gatcctgtat tatctgctag actcatgacg actaattcag
     1621 tcgttaatgg atctaaaccc ctttctatac attcgacact tgcgtaaaag cttttcatat
     1681 cgatcaaaaa ggaagtattt actggctctt ttgaataatc tatcataaat aacactccat
     1741 tcgtcacaat tatacaaaca tatgttccct tttgtaaaga aggaagttca aatgaacaaa
     1801 aaaagattcg aattgttatt acaaaaagtt taaacggata actgaccact tattagattt
     1861 taaatgcgtt agaatcttca tatatcaact aattagaata aaaaaagcaa agtaactaat
     1921 gaaaatccat tagttacttt gctttttatt aaaaattttc aatcgttaaa ttactaaagt
     1981 tttttagacc actagcaaga cagatacaaa ataatgaatg aattgaaagt tattgttcca
     2041 attctctttt taattgagga aaatcattaa gtaattcaaa taaaatacta aagtttgaga
     2101 gaaaactcac atccttatta ggattttctg aattattcat tccatataat atttttaatc
     2161 ttttgaaaac ttccttcatg ctttcatttt ctgcatattt actttctaga gataaatctg
     2221 agagataaa
//
LOCUS       DAZKAO010000219         2145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000219 DAZKAO010000000
VERSION     DAZKAO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.227"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG8H8_002791"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG8H8_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG8H8_002792"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG8H8_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG8H8_002793"
     CDS             1898..2059
                     /locus_tag="ACG8H8_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987794.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          774 a          332 c          364 g          675 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaa
//
LOCUS       DAZKAO010000220         2077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000220 DAZKAO010000000
VERSION     DAZKAO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.228"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8H8_002794"
     CDS             <1..716
                     /locus_tag="ACG8H8_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP8987795.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
     gene            915..>2077
                     /locus_tag="ACG8H8_002795"
     CDS             915..>2077
                     /locus_tag="ACG8H8_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8987796.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYS"
BASE COUNT          677 a          402 c          409 g          589 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      841 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
      901 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
      961 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1021 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1081 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1141 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1201 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1261 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
     1321 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
     1381 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
     1441 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
     1501 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
     1561 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
     1621 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
     1681 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
     1741 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
     1801 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
     1861 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1921 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1981 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     2041 ttctgccaca gatccaaaat gcgcttcact acagcta
//
LOCUS       DAZKAO010000221         2056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000221 DAZKAO010000000
VERSION     DAZKAO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.230"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACG8H8_002796"
     CDS             493..714
                     /locus_tag="ACG8H8_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987797.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACG8H8_002797"
     CDS             715..972
                     /locus_tag="ACG8H8_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987798.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACG8H8_002798"
     CDS             complement(1108..1710)
                     /locus_tag="ACG8H8_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP8987799.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACG8H8_002799"
     CDS             complement(1777..1932)
                     /locus_tag="ACG8H8_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP8987800.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAZKAO010000222         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000222 DAZKAO010000000
VERSION     DAZKAO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.231"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8H8_002800"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8H8_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8H8_002801"
     CDS             complement(728..1744)
                     /locus_tag="ACG8H8_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8987801.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKAO010000223         1949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000223 DAZKAO010000000
VERSION     DAZKAO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.232"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1435
                     /locus_tag="ACG8H8_002802"
     CDS             476..1435
                     /locus_tag="ACG8H8_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987802.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1422..>1949)
                     /locus_tag="ACG8H8_002803"
     CDS             complement(1422..>1949)
                     /locus_tag="ACG8H8_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987803.1"
                     /translation="NRQSFLIYAINNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIE
                     YQLSVGEIKCVFEEDLELDLKKIFASIHDYIGLIYKIIEDDKQTELNMCYFDREQDFF
                     YYGGMGGGSIVPKINLVELDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIE
                     YSIEYFYKVSFVKLS"
BASE COUNT          663 a          327 c          299 g          660 t
ORIGIN      
        1 taagatgaga gtcattaact tatgatagta gtcaatttgc ttttcactaa ttacaaaata
       61 tgtgatatag gaagtttagt tattgttctc ataatgccat cctgttttta gattatctag
      121 taattttttt gttatttaaa attaggagca gtgccaattg ttttaatttt aaaggctttt
      181 tttatatctt ctaaattaga agatatttgt atatcaatat ggaatatatt accttttttg
      241 tattttttta tgttatcttt agctaaaaca tcaaatacat gtccttgaaa ttgtatcgag
      301 gcagctggat ttagtttaaa ttgaggaaac tttaccacag ctgattcaaa acacttgtca
      361 cattttttta tttttttacc ttcgccgatt gtaaaattaa gctagacaac taaaaaagtc
      421 atttgtgcta cactcaaaat agttccgtcg aaagaattat aaggagtgag tacaaatgac
      481 ctatacccat cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca
      541 ctctgtttca aaagtagccc atctggtaca acgttctcga caaaccattt ataatgtcta
      601 tcgttttctt aaatctggtg gaagcgcaat aagctactat gaacaatata agaaaaacaa
      661 acgtaactgc ggaagacgtc cgattgtgtt accagatggg caacaagaat acattcaaaa
      721 aaaggtggct caagggtgga ctccagatgt ccttattggt cgagcagagt ttcctattgc
      781 ttgttcggta cgtacactat atcggatgtt taaagataaa acctttgacc ctcacgattt
      841 accaatgaaa gggaaaagaa aaccaaacgg acataaagaa aaaagaggaa aacaagcttt
      901 tcgccgttcg attcaggata gaacaactga ttatatacag ttcaatcatg agtttggtca
      961 cctagaaggt gacaccattg tcggtggaaa acataaaagt gcggtgatca cgttagttga
     1021 gcgattatct aaagtgatca tcaccttgaa acccgctggt aggcaagcta tagatattga
     1081 gaatagactg aatcaatggt ttcaatctgt accgaaacac ttgtttaaat ccatcacatt
     1141 tgattgtgga aaagagttct cgaattggaa aaagattagt aataccaatg atatttcgat
     1201 ttatttcgct gatccaggaa caccttctca acgaggacta aatgaacatt cgaatggctt
     1261 attacgtaaa gatggcttat ataaagctat ggattttaat aaggtatccg aaacctttat
     1321 ccaatcggtg gcatcaaaac gaaatcatat tcctagaaaa tcattacact atcgcacacc
     1381 attggaagta tttttgagtt acgtaggtaa agatgatttg tctagcttaa tttgacaaac
     1441 gaaactttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
     1501 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa aatctaaact attaaaaaca
     1561 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
     1621 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
     1681 agctcagttt gtttatcatc ctcaataatt ttataaatca aacctatata atcatgaatg
     1741 ctggcaaaaa ttttttttaa gtcaagttct aaatcttctt cgaaaacaca tttaatttct
     1801 cctactgata attgatactc tatattacat gacttaatta gttttttaga tcccccctcc
     1861 agataggcat atgtttcaat aattatgttc tttttattgc ttggcaaatt tccgttattt
     1921 attgcataaa ttaaaaatga ctgtctatt
//
LOCUS       DAZKAO010000224         1934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000224 DAZKAO010000000
VERSION     DAZKAO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.233"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..215)
                     /locus_tag="ACG8H8_002804"
     CDS             complement(<1..215)
                     /locus_tag="ACG8H8_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFP8987804.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLL"
     gene            complement(208..1170)
                     /locus_tag="ACG8H8_002805"
     CDS             complement(208..1170)
                     /locus_tag="ACG8H8_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFP8987805.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            complement(1203..>1934)
                     /locus_tag="ACG8H8_002806"
     CDS             complement(1203..>1934)
                     /locus_tag="ACG8H8_002806"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8987806.1"
                     /translation="EDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHP
                     FEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEI
                     LKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSL
                     YEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANK
                     ITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          645 a          328 c          210 g          751 t
ORIGIN      
        1 agtagccaag aaaaacgcat tttaaaaaaa tttttcttta taaaaacctc atacaaaact
       61 ataattccac cccaagcaaa agctattttc atcgggatat tagttacatt aaaagtacta
      121 ggcataaaat aaagcagaca aatgattaaa tttattactc gaaacgcatc tcgacttaat
      181 aaactcttct ctaaaacagg tactattcta ttcatctata tttccaactt tcaaaatttc
      241 tttatcaatt ctatttaata gtttatcaga gtaatccgat ttaacttttc ttacaaataa
      301 tttttcactt tcttttattt gatctatatc cctttcatca aaccatttgt tcatggaagg
      361 atcaataata tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt
      421 caattcaagt ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa
      481 aaaaagtgta tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta
      541 gtcttgttga ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc
      601 ccaccattga gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt
      661 aatcatcctc agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgttatt
      721 cctattaatt ctaggataat catgtttttg atatacatct atatgtttat aataaaattc
      781 tttatgttta atttgatcta gatctattcc acgaataaac tccactgaac tattcttttt
      841 aaaataatta taaatatact cattatcttt gattggataa tcagcaccag ataataatac
      901 atattttaaa tatttctcat tttgtaatgc tctctttact aaattgattt ctgcttgtac
      961 aatggaaaaa ccaccccaat aaattttaac acggttttta ttacataaaa attcaacatt
     1021 atcatatgat ttaaatgctt caataaatgg ttctatatca gtttttttat ctatatgaat
     1081 aataaaaact gcattttcgt tcaatcgatt gaccaatctc tttaagttgc caatatcatt
     1141 gtatgccaat atcaaataac atattttcaa aaataatcac tcctaaaata tcttgaacat
     1201 ttttaatgat ctctactata tatactctcc atttgttcag tagtgtacga aacatcataa
     1261 ctctttagtt cttgtgttat tttattagca tacggctctt ttattacttt ttttgcccat
     1321 tcttgtggtg tttcatctaa tgatacaaaa tccacattat ttgttaactt tgtatctttg
     1381 gaaatagtat ctgctaagac taattttaat tttgctgctt gtgcttctaa tccaaccatt
     1441 ggcagacctt cataaagaga aggcattaca aaaacatcca tcacagataa taactcggga
     1501 atatcgtttc tttctcctaa aaattttact cttttctcta tatccaattc tactgctttg
     1561 gctttcagtt tatttctaag tgacccttca cctactaaaa tcaagtaaga atcagaattc
     1621 atttttagta tttctaaaaa aatctctaac aaataagatt gatttttttg tttctctaat
     1681 cgtccaatgt ttcccacaac aaaagtactc attgagatat ccaattcttt tctcttatta
     1741 accttagctt ctgaattttt actataaagc tctaaatcaa taccattatg aactatctca
     1801 aaggggtggt tttgaaacag actttcacca gcttcttgac taacggcaag gtagtctgtt
     1861 gcattttgta acaacaattt ggttaaaaat ttacgcaata cattcattac aatattttga
     1921 gattttccat cttc
//
LOCUS       DAZKAO010000225         1857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000225 DAZKAO010000000
VERSION     DAZKAO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.234"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..735)
                     /locus_tag="ACG8H8_002807"
     CDS             complement(34..735)
                     /locus_tag="ACG8H8_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8987807.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ACG8H8_002808"
     CDS             complement(751..1191)
                     /locus_tag="ACG8H8_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP8987808.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ACG8H8_002809"
     CDS             complement(1191..1766)
                     /locus_tag="ACG8H8_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP8987809.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          520 a          400 c          288 g          649 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtat
//
LOCUS       DAZKAO010000226         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000226 DAZKAO010000000
VERSION     DAZKAO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.235"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8H8_002810"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8H8_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFP8987810.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8H8_002811"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8H8_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP8987811.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZKAO010000227         1815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000227 DAZKAO010000000
VERSION     DAZKAO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.236"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..505
                     /locus_tag="ACG8H8_002812"
     CDS             23..505
                     /locus_tag="ACG8H8_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987812.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            702..1592
                     /locus_tag="ACG8H8_002813"
     CDS             702..1592
                     /locus_tag="ACG8H8_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP8987813.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          593 a          313 c          379 g          530 t
ORIGIN      
        1 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
       61 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
      121 tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc
      181 tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
      241 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
      301 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattt
      361 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
      421 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
      481 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaacat atgagctgcg
      541 taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt
      601 acatgatttt tacataactt aactatattt ttacagcaac catacgaggg atttacagtc
      661 gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta
      721 ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg
      781 gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag
      841 gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc
      901 caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag
      961 ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg
     1021 cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag
     1081 atacagatgt caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca
     1141 ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg
     1201 gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt
     1261 cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa
     1321 tcagctattt agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg
     1381 tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata
     1441 tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag
     1501 aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag
     1561 tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg
     1621 ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct ttaattgtga
     1681 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     1741 accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtaacttttt ttggctcttc
     1801 gtcaaatcgt gttgg
//
LOCUS       DAZKAO010000228         1808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000228 DAZKAO010000000
VERSION     DAZKAO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.237"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8H8_002814"
     CDS             275..1753
                     /locus_tag="ACG8H8_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987814.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          654 a          252 c          322 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gttttttt
//
LOCUS       DAZKAO010000229         1807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000229 DAZKAO010000000
VERSION     DAZKAO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.238"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACG8H8_002815"
     CDS             complement(<1..626)
                     /locus_tag="ACG8H8_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8987815.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELI"
     gene            complement(637..1305)
                     /locus_tag="ACG8H8_002816"
     CDS             complement(637..1305)
                     /locus_tag="ACG8H8_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP8987816.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1436..>1807)
                     /locus_tag="ACG8H8_002817"
     CDS             complement(1436..>1807)
                     /locus_tag="ACG8H8_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8987817.1"
                     /translation="IDIEKSWRENFNELDPFINYIFENKRIFDLVLFQSAGTKYQDIV
                     EKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEK
                     TMLLTRRLYQFSVPGWIELLS"
BASE COUNT          657 a          301 c          232 g          617 t
ORIGIN      
        1 tcaattaatt ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta
       61 ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa
      121 gacggttcat ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt
      181 ttctgccgtt caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct
      241 aataattcag ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt
      301 tccatcgaaa atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct
      361 tgacttacgt agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta
      421 ttttcaaata aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc
      481 attaacttta atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagacta
      541 ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa
      601 aaagaaacat tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa
      661 aatagtaacg gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga
      721 acttcttttc acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc
      781 aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg
      841 aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga
      901 acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa
      961 atgtattttt tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca
     1021 tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcc caaaaaatat
     1081 agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata
     1141 aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc
     1201 aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa
     1261 aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa
     1321 aaataagatc gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg
     1381 cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt tttaattaac
     1441 tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag
     1501 tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa
     1561 tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct
     1621 ttaacttatc tagacaacag actgtctttt ttgtcacaaa tgatgtaatt ttttcaacaa
     1681 tatcttgata tttggttcca gctgattgga ataaaaccaa atcgaatata cgtttattct
     1741 caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta
     1801 tatctat
//
LOCUS       DAZKAO010000230         1797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000230 DAZKAO010000000
VERSION     DAZKAO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.239"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..1367)
                     /locus_tag="ACG8H8_002818"
                     /pseudo
     CDS             complement(189..1367)
                     /locus_tag="ACG8H8_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1397..>1797
                     /locus_tag="ACG8H8_002819"
     CDS             1397..>1797
                     /locus_tag="ACG8H8_002819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987818.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLP"
BASE COUNT          555 a          312 c          335 g          595 t
ORIGIN      
        1 catattgtac atcttatgaa gacttaatga ctacttgtag tcaatagatt taaagtattt
       61 attttctcat ttatgtatca agaataaata ctgatataat aaaaattata tcaatgtttt
      121 gatgaagtaa tttggaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
      181 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      241 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      301 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttcttg
      361 aaagattcga ttaaattagt tgagtaaatg cttcggcgga taccaggcgg aaactcataa
      421 aaagtcaata aatcttgatt ttttatcagt gactgcgtca ctttaggata agttttttgc
      481 tacttctcaa tcatactccc taaaaaggta tttgcctctt cctttgagct agcttgataa
      541 acagccttaa aatcatcaca gatttctttt cgatctttga cacgtacttt atgagcaata
      601 ttacgggaaa cgtgaacaca gcaatgttgg tatttagctt taggataaat ctggtgaatc
      661 gtatctttca tgccttttaa gccatcggta ataaacagta agacatcatg aacccctctg
      721 gagtaaatgt cctgtaacaa ctcattccaa acgtaggtcg attcagtcgg agcaatcgca
      781 taacttagta cttctttagt gccgtcttct cgtataccaa tggcaatata gattgcttct
      841 ttggagacgg tttgccgttt taaaggaata taagtagcat ccatgaaaat agcgacatac
      901 ttatcattta gagttctaac tttaaatgca tttacttctt cagtcagcac tttagtcatg
      961 ttggacatgg tttggggcgt gtagtgatgc ccatacattt tctcaatcaa gtctgcgatt
     1021 tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa gatatcattc
     1081 gtccgtctat aagcaggaac agtctgttgc ttgaactcgc cgttgcggtc gcgcggaatc
     1141 tggagatgaa gttccccgta ctcggtcttg accgtacggt cataggagcc gttacgagaa
     1201 ttccccgtgt taaaaccaat gcgatcgtac ttttcgtaat cgaggaaagc cgttaactcc
     1261 gttttgagaa gcgtattgac agctttttcc aaatggacac ggaataattc attcaaatcg
     1321 cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatatg gaagtcctct
     1381 tttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt tttctattta
     1441 cacaaaatat tttacactct cgtcaaatat aaaaactagt acgaaattaa tttgtaaatt
     1501 atttaagctt gataaaataa gcaatatcga tgatgtaaag tccaagatat taacaagaaa
     1561 agtaagcgaa ccagtaaagc taacggcaca agataaaaaa aatggaattg ttctttatgg
     1621 tgatgatgca aagtataact ataattggga tacctcctca gaagctgagg tattaagtat
     1681 tcctactacc gatacttcag gggaaaaagt cacctttaaa ataaatttta taacggcaat
     1741 ttctaagttg gagtatggga agcaaaaaaa gagagataac aatgatcaat atctacc
//
LOCUS       DAZKAO010000231         1749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000231 DAZKAO010000000
VERSION     DAZKAO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.240"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8H8_002820"
     CDS             134..307
                     /locus_tag="ACG8H8_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987819.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG8H8_002821"
     CDS             947..1420
                     /locus_tag="ACG8H8_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP8987820.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG8H8_002822"
     CDS             1429..1656
                     /locus_tag="ACG8H8_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987821.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          587 a          272 c          260 g          630 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttt
//
LOCUS       DAZKAO010000232         1654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000232 DAZKAO010000000
VERSION     DAZKAO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.241"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1144
                     /locus_tag="ACG8H8_002823"
     CDS             <1..1144
                     /locus_tag="ACG8H8_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8987822.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1425..1654
                     /locus_tag="ACG8H8_002824"
                     /pseudo
     CDS             1425..1654
                     /locus_tag="ACG8H8_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          414 t
ORIGIN      
        1 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
       61 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
      121 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
      181 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
      241 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
      301 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
      361 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
      421 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
      481 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
      541 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
      601 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
      661 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
      721 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
      781 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
      841 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
      901 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
      961 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
     1021 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
     1081 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
     1141 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
     1201 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
     1261 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
     1321 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
     1381 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
     1441 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
     1501 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1561 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
     1621 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAZKAO010000233         1646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000233 DAZKAO010000000
VERSION     DAZKAO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.242"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..487
                     /locus_tag="ACG8H8_002825"
     CDS             113..487
                     /locus_tag="ACG8H8_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8987823.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(601..1230)
                     /locus_tag="ACG8H8_002826"
     CDS             complement(601..1230)
                     /locus_tag="ACG8H8_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8987824.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1364..1436)
                     /locus_tag="ACG8H8_002827"
     tRNA            complement(1364..1436)
                     /locus_tag="ACG8H8_002827"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1401..1403),aa:Thr,seq:ggt)
BASE COUNT          578 a          236 c          280 g          552 t
ORIGIN      
        1 gaaatatcat aagatatttc ttacatcttt ttgttttgag caggacttta gataatttta
       61 ttaatatatg ctagcatgct tttgtaagaa aaagaaagag ggcgtattag ttatgtatct
      121 taaaattacg gaacaagcac aagaaagatt acaaaaatat attgatgaag gcgcaacagt
      181 tatattggat ttagatgatg gcgttgggga atattctaaa atgggtgttt gctctttaga
      241 tactagtttt cgtttacttc ttcttgataa agaacagaga aaaaatgatt ataaactcca
      301 attagatagt gatattgggg atgtttatat aaaagattat tcaaaaatgt acatggatga
      361 acatatgacc ctttctttag atccacgtct aggtgtattt actcttgaaa gcccaagtgg
      421 ttctttagac agtcatgttc aattagtaga tctacgacca gaaaaattct ctggaataaa
      481 acaataactt tctctctatt gtccataaaa ctagtctcaa tcaagctgta ttgaaaaaac
      541 aagacaacat tcttaccatg gtaaataaaa agacgacaca ttttgtcgtc tttttttaaa
      601 ttaactattt tcgataaaag atcgcatagc ttgtgccatt atttctggct tttctctatt
      661 caattcatgt tttccgtttt taataaagag tagccggcca ttcgttgaaa gtttatataa
      721 acgttttgct gctggtttat tcgcatgatc tttggtaccg cataaaatca atttaggaag
      781 tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca agtgaacgaa gtagggtaag
      841 ggtttgtttt ttagatagac ctaatttttc aaatatctta gaaggtacgc agtaaaaata
      901 aatattctgg aaagtaagca ataagcgatt aggagactca aattgtggag cgactaaaac
      961 aacctttttt aaatatggag aaggagttgc aagatagttt aaagcaataa cagcgcctaa
     1021 agataatcca cataaaataa aaggttcctt tatctgattc aattgtactt cgatgtattt
     1081 ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa tgattgaaca atgaaagact
     1141 tttgcaagaa taactattgg gtagataaga gattacatct ttccatgatt gttcgtcttg
     1201 gcctagacca tgcaaaaaaa caattttcat attgctctcc tttttatagt tatcatatag
     1261 actttctttg tactattaaa atataaacat tcatttctta cgtcataaaa taaaaaaaca
     1321 gcgcagacac attgttaaac caacatgttg cactgtttaa ggaagccact tgccgggctt
     1381 gaaccggcga cctcttcctt accatggaag cgctctacca gctgagctaa agcggcatta
     1441 tcattaatac aaataatagg ataacgaata ttaatgtatt tgtcaacaga attttttcga
     1501 aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa aataaaaata acaaaaaatg
     1561 cgaacggttt ctttgataat ataataaaaa aattcaaaaa aagtattgcg ttatttagaa
     1621 gaaattgata ttatattcat gtactt
//
LOCUS       DAZKAO010000234         1644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000234 DAZKAO010000000
VERSION     DAZKAO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.243"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8H8_002828"
     CDS             complement(52..1518)
                     /locus_tag="ACG8H8_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP8987825.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          552 a          337 c          296 g          459 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaa
//
LOCUS       DAZKAO010000235         1561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000235 DAZKAO010000000
VERSION     DAZKAO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.245"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..546)
                     /locus_tag="ACG8H8_002829"
     CDS             complement(<1..546)
                     /locus_tag="ACG8H8_002829"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987826.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINY"
     gene            710..916
                     /locus_tag="ACG8H8_002830"
     CDS             710..916
                     /locus_tag="ACG8H8_002830"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8987827.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            967..1248
                     /locus_tag="ACG8H8_002831"
     CDS             967..1248
                     /locus_tag="ACG8H8_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987828.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          584 a          194 c          276 g          507 t
ORIGIN      
        1 ataattgata cttttaagac ttttctgtct gtttgaaagt ttttcatcaa gacaaatata
       61 tatgaaaccc aggatgagtt ggtcatctga tttatcagtt tgcaagagtt ttttatatac
      121 atcatctata atcatttcta caaagttatc ttttaaagaa ggtgatttat ctagactata
      181 tgttttcgat actgctaaaa ataagtcata tagaccaagc ctatctctgt ctttaccgaa
      241 atctttgaat ttgttatgga aggtaccgtt gtcaaaaaaa atctcattga ataaatactt
      301 gatatatttc ttagtttttt gagaattacc tgtcagtaga tattcagtag aaacaccacc
      361 aagtttagca atacttagta gccgcgcttt gttgggtatg ttctttcctt tttcccatct
      421 agaaacattg cttttgcttg cgggaggtgt cagattttcg ccaaattctt caagagtata
      481 ccctaaagaa acacgtattt cttgtattcg ttttccaatt tcagaattaa tgtttttatc
      541 aaacaaaatg gtttcaccac cttcatctat tctattctac agtttacact acattctcat
      601 ataatgaaat gaaaaagtag ataaaagttg aattttacgt tgcggtatga ataaatatat
      661 gttattcttt tagtgacgaa aagttgaaaa gaaggaggag tgaggagtca tgaagcctaa
      721 agattttaga aagttttgtg gattatcaca atctgaactt gctgaaaaat atggcatttc
      781 agtacaagcg gtaagaaaca aagaaagcgg taaaacaaac tattctccag aagaaatgat
      841 tattttgaag tatcttgtga aagaaaaagg aattgaaaat atcacaattg atcaaatttt
      901 ttttaatgaa aagtgacgaa aagttgaatt tgaatacatg tagtcaaatt agtgggggca
      961 aagaaaatga aatctgagat tcaagttatt cttccatcgg atttagatca agaattgcga
     1021 agatatttct atcgagttgc aaaagaagca gtagatcaag ttcgaattga aacatctcta
     1081 agcaaagaag tttttagatt gggggaagct tgtaagtatt taagctgtag cagaagtaca
     1141 ttaaaaaaat ttatcagaga gaaaggttta aaagtaagca taatcggaga ttgtcaattt
     1201 attagaaaag aagaattagc aaggtttatc aaagaatatg aagtataaaa agttgcgtgg
     1261 cgtgcttttg atagaaaaaa ggaagtgttt gtttgaataa tgatgaagta tagaaaatat
     1321 aaaaatactt tgaaaaaaat taaaaggagt gttcccgaca gtttggcgac cacaggaaac
     1381 actcgacaat aaagttctaa gaaaacttta tttgactaca ttttaacatt tattagaaag
     1441 aagaggaata aatggaagaa atagataaat taattgctca gtatgcatac tcaaaagatg
     1501 acgtatatgc tgaaaaatac ttgaagtatc cgattttttt agataaacat aaaaaatata
     1561 a
//
LOCUS       DAZKAO010000236         1549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000236 DAZKAO010000000
VERSION     DAZKAO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.246"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACG8H8_002832"
     CDS             complement(<1..337)
                     /locus_tag="ACG8H8_002832"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8987829.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPT
                     KKNGWCYLSS"
     gene            complement(448..702)
                     /locus_tag="ACG8H8_002833"
     CDS             complement(448..702)
                     /locus_tag="ACG8H8_002833"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987830.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            834..1343
                     /locus_tag="ACG8H8_002834"
                     /pseudo
     CDS             834..1343
                     /locus_tag="ACG8H8_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1344..>1549
                     /locus_tag="ACG8H8_002835"
     CDS             1344..>1549
                     /locus_tag="ACG8H8_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFP8987831.1"
                     /translation="MLSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYIIQKDK
                     VSEEMIYYIGKQKQIILKDPIPLK"
BASE COUNT          541 a          270 c          259 g          479 t
ORIGIN      
        1 ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg tcagctgccc
       61 atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct tttgaaatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 actttttgag ccaaaagtaa ggaattacaa aaagagaaag aagagaagga agaatgaaaa
      841 aggtattatt tgcggttagt ttagttgtta atatagcact agcaggagga ttattttata
      901 ccattacaga aatgaataag cttgatgacc aacgggaaag cttagcaatc agtaacgaac
      961 aatacaaaag gaagctagat taagctagca gcaccaacgt ggacactcaa gaagccgtag
     1021 aatcctctac aattgtttat agcaatgaat ccagtgaaac cctaacaaat tcggaaagaa
     1081 gcacagctcc agctacagaa actgtttctc aaaaagctac tataacaata ggaagtaaag
     1141 tattcacgtt tatagtaaat ggcttgacaa tagaacacga ttctagcaga acaggaaaaa
     1201 tgaccgagct ttctcacgag tttccagtga ttgttactac tcctagtgct acccttaacg
     1261 gtatattggt gcaagacaac aatgataata attgctatat aatggtagac ggtcaaactc
     1321 tatacagagg aacataccaa taaattttga gtctcaaaga tatttgtcac tacggtaaac
     1381 ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg
     1441 tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg atttattaca
     1501 ttgggaaaca aaagcaaata attttaaaag atcctatccc cttaaaaga
//
LOCUS       DAZKAO010000237         1524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000237 DAZKAO010000000
VERSION     DAZKAO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.247"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..1522
                     /locus_tag="ACG8H8_002836"
     CDS             join(183..624,624..1522)
                     /locus_tag="ACG8H8_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP8987832.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          609 a          204 c          268 g          443 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt cactagaaat ctaattaaaa ggggtgcttt
      181 ttttgcggaa atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg
      241 aaggcggcta caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt
      301 gggtaaggat ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca
      361 ctacaaaatt taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag
      421 aaactgctaa aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat
      481 atgaagaagg cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga
      541 attccaagct aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc
      601 gtttaagagc agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt
      661 tgagcaagaa gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa
      721 tctattactt tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt
      781 agataagcca gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc
      841 aagaaactct tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat
      901 caatcataaa acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat
      961 aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg
     1021 aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat
     1081 aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag aaataattag
     1141 ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat
     1201 taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca
     1261 gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa
     1321 aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt
     1381 tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat
     1441 taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata
     1501 tcggaaacag tccattaaat agtt
//
LOCUS       DAZKAO010000238         1488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000238 DAZKAO010000000
VERSION     DAZKAO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.248"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            873..1085
                     /locus_tag="ACG8H8_002837"
     CDS             873..1085
                     /locus_tag="ACG8H8_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8987833.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1075..1470
                     /locus_tag="ACG8H8_002838"
     CDS             1075..1470
                     /locus_tag="ACG8H8_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987834.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          519 a          220 c          251 g          498 t
ORIGIN      
        1 tgatccctaa taggtgtaaa acagttataa gaaatgagta ctctagtatt tgtgttaccc
       61 gaataattac ttgattatat tggtttccca tataattgaa ggctgtccta atttttacct
      121 actatatgat cataattgat taactcttat atatttccga aaattatctt gaagatttct
      181 attggtatgg agaatatcca tttcattgcc aataaggata taaattctca aaataacaat
      241 cgatgtctgt attaaacaag cctagaatgg taaatctgat tcgccaaaac cattggattg
      301 tatcgatttg ctttacgcga acgccaccta cccattttat aaaaataaaa tgggcaaaag
      361 atctttcttt ttgtcataca aaaagaagaa aaaaaagaga cgattttctt cttccaaaat
      421 cgtccctctt ttttctaaaa ccatggatga acgaacaaaa aatctctcgt attttgtttg
      481 ttcatccact aaaaatatgc gaacagtatg gtttcattca atcctgttct tacaagtcaa
      541 aagcatcgaa tcgtccttgg acgattcaac aaaaaaagta gtcgtttttg gatgactact
      601 aagttctaaa cattcattac ccaaaatcaa tttggtctaa catcgtttct cgactggctt
      661 gatctaatcg gctcttgaca gggctttttc ttttctgttg atagcagata agaggaaata
      721 agaataagtg tgttcatgtg tcataaaaac agtaactaaa acattaaaat caaaatttat
      781 atggaaagct tccttgacat tttgtgctcg caggagtatt ataaaagcac gaaaggaaag
      841 taaactttcc attttttaga aaggagcatg ttgtgaaaaa tcgtttggaa gaattacgaa
      901 aacaaagagg aattaaacaa gaagatttag cttctgctct tgaagtatct cgtcaaacaa
      961 taggttcttt agaaaatgga cgttataatc cctctattat attggcattt aaaattgcaa
     1021 ggtactttca aatgagcatt gaagaaattt ttatctatga ggaggaaagt gaagatgaaa
     1081 aataagggtc agatttggta tatcggttat attgtttcgt tgtttttgat tgtgacaatt
     1141 ttggttacag attttcctaa aatggtagat attggacttg ctattttatt cagtgccgtt
     1201 tttggcgttt ctcatgttca aattttgcat gataaaatga ttaaaaatga tgcagactac
     1261 aaaatcaatg ttatggacga acggaatatc ataatcaaag aaaaagcggg aaatataaca
     1321 aatatgttta acacaatact gttaggggtg gcaacagtta tatttatttg tttagactac
     1381 atcattccag ctattattgt cggaagtatc attgcaatcc agccgattat tttaatcatt
     1441 atcagtagtg tgcttgagaa aaaaatgtag aaataaaggt tcatacaa
//
LOCUS       DAZKAO010000239         1485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000239 DAZKAO010000000
VERSION     DAZKAO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.249"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACG8H8_002839"
     CDS             <1..675
                     /locus_tag="ACG8H8_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP8987835.1"
                     /translation="QIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSE
                     AVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAV
                     AMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELS
                     TKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVL
                     LEEDVE"
     gene            672..>1485
                     /gene="neuC"
                     /locus_tag="ACG8H8_002840"
     CDS             672..>1485
                     /gene="neuC"
                     /locus_tag="ACG8H8_002840"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP8987836.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPE"
BASE COUNT          519 a          194 c          303 g          469 t
ORIGIN      
        1 caaattggtg tacaaggatt taaaattggc tcaggagatt taacaaattt accttttctt
       61 agaaaaatag atatgtatgg gttgccagtt ttattatcta caggtatggc tgatattcat
      121 gaggtcagtg aggctgtaga ttgtttcata gatagtccag ttactatttt acattgtact
      181 tcaaattatc cggctagttc tgaagatatt aatttattgg cgattaacac attaaaagaa
      241 atgttcggga tacccgttgg gtactccgat cattcattgg gatatgatgt tggtatttgt
      301 gctgttgcga tgggtgctaa ggtaattgag aaacatttta ccttggataa ggaactacct
      361 ggtccagatc ataaagcttc tttagatcct caggaatttg cagattttgt aaagcatatt
      421 agaaacactg aaatattttt aggtgatggt gttaagcgag caatgccttc tgaacttagt
      481 acaaaagaag tatcaagaaa aagcctagtg gtcactgaga atttaaatgt gggagatatt
      541 cttacagaga aatctcttac aataaaacgt cctggaaatg gaatagctcc taagttttta
      601 tatcaatata ttggcaaaaa agttaaaaga ccaataagtg cagatagtgt tctgcttgag
      661 gaagatgttg aatgaaaaaa atacttatag taagtggaac acgagctgat tatggaatat
      721 ataaacctgt tgctgagaat ttactacaag ataattggga tgtatcattt gttgtttctg
      781 gaatgcattt atcccataaa tatggattta ctaaagaatt gatcttagct gacggattta
      841 gaattagtgg agaagttcaa agtttgtttt tagagaacaa taagggaaac atggctcggt
      901 ctgtggcatt agagataaat ggtttcacaa atatatttga acaaactagc cctgattttg
      961 tattattact aggggataga ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg
     1021 gaatcaaaac agcacatatt cacggaggcg aagtgtctgg tactgtagat gaatcaataa
     1081 gacatgccat aacgaaaata gcatctgttc attttgctgc tacggaaaaa agcaaagagc
     1141 gtttaataaa aatgggggaa gattcttgga gagtttttcc cgtcggagct cctagaatag
     1201 atactatttt aaataataga ttacctagtt ttagagatat aaaagaaaaa tatcatctaa
     1261 aatttgaaaa gaaaaaatac gttttgcaag tatttcatcc agttgtaacc gaactagaaa
     1321 atatagaaaa acaaatttta gctttgatca attcaatgga acaacaatct caaccaatta
     1381 tttgcatttt acctaatagt gatgctggta gcgaaattat cagaaaaatg taccagaaat
     1441 atttaaaaac ggatattatt tatattacga atttagagcc ggagg
//
LOCUS       DAZKAO010000240         1484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000240 DAZKAO010000000
VERSION     DAZKAO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.250"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG8H8_002841"
     CDS             64..972
                     /locus_tag="ACG8H8_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFP8987837.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1484
                     /locus_tag="ACG8H8_002842"
     CDS             972..>1484
                     /locus_tag="ACG8H8_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987838.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVN"
BASE COUNT          531 a          197 c          316 g          440 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taat
//
LOCUS       DAZKAO010000241         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000241 DAZKAO010000000
VERSION     DAZKAO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.251"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8H8_002843"
     CDS             complement(150..1337)
                     /locus_tag="ACG8H8_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP8987839.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKAO010000242         1347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000242 DAZKAO010000000
VERSION     DAZKAO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.252"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8H8_002844"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8H8_002844"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP8987840.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..>1347
                     /locus_tag="ACG8H8_002845"
     CDS             1131..>1347
                     /locus_tag="ACG8H8_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987841.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMK"
BASE COUNT          411 a          272 c          204 g          460 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaa
//
LOCUS       DAZKAO010000243         1337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000243 DAZKAO010000000
VERSION     DAZKAO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.253"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG8H8_002846"
     rRNA            <1..1337
                     /locus_tag="ACG8H8_002846"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZKAO010000244         1334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000244 DAZKAO010000000
VERSION     DAZKAO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.254"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1165
                     /locus_tag="ACG8H8_002847"
     CDS             86..1165
                     /locus_tag="ACG8H8_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987842.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAZKAO010000245         1332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000245 DAZKAO010000000
VERSION     DAZKAO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.255"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1332
                     /locus_tag="ACG8H8_002848"
     CDS             82..>1332
                     /locus_tag="ACG8H8_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP8987843.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENM"
BASE COUNT          452 a          259 c          254 g          367 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tg
//
LOCUS       DAZKAO010000246         1315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000246 DAZKAO010000000
VERSION     DAZKAO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.256"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACG8H8_002849"
     CDS             <1..276
                     /locus_tag="ACG8H8_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP8987844.1"
                     /translation="EVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQ
                     CGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            281..1228
                     /locus_tag="ACG8H8_002850"
     CDS             281..1228
                     /locus_tag="ACG8H8_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP8987845.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          436 a          189 c          271 g          419 t
ORIGIN      
        1 gaagttacta tagttaataa atcaagatac acatctaggc aacagacacc tgaagttttt
       61 gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag taattatatt
      121 tttgatgggc aatgtgggat aataaaaatg agagattatc taattttaga tattgatagt
      181 gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag
      241 cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac
      301 cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt
      361 aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat
      421 cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa
      481 taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg
      541 accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg
      601 caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga
      661 tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt
      721 tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg
      781 tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag
      841 tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc
      901 atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat
      961 ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg
     1021 aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt
     1081 agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga
     1141 tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac
     1201 taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc
     1261 aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga tagaa
//
LOCUS       DAZKAO010000247         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000247 DAZKAO010000000
VERSION     DAZKAO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.257"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1145)
                     /locus_tag="ACG8H8_002851"
     CDS             complement(105..1145)
                     /locus_tag="ACG8H8_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8987846.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          382 a          203 c          180 g          519 t
ORIGIN      
        1 aaaatagcta tttctttgta acatttgtaa tataattaga ataataaaaa atttttaatt
       61 ttaatgctac tccatgaagc aacaatcaat ttgtcagtga aatttcactc taaccagtct
      121 ttcgtaatag tttttgggaa gctttcagaa ttcaaacgtt tttcacttga tactttagca
      181 gctattggtt tttcgataaa ccaatttttt acatagacaa aaatcaggtt ctggatattt
      241 tcaacttttt cggtttttaa tttttgatag gcttttaaca gtgtcgtgta taattcttgt
      301 tctgcattga cataataact atcgagttct tcaaaaatta ttgaaatacc actttctgtt
      361 tcggcttttt tctttgcatt atgaatagtc tggacagttt tttgagcttc actgtaactt
      421 gaagaaaatg tttttattaa atttaatact cgttcaggga taaacgtagc aatgctgtcg
      481 tttgtcataa ttgtctcaaa attgtctaac aacaattgat cttgtaggcg gtctttttca
      541 gtgtctatat tgtgtctatt agtgtctaat atattattat taagttctgg tatgattttc
      601 ataccctctg atttatcaac gttttggcac ggtatgattt tcataccctc ttgagcccca
      661 agggattcag cgtctgattt ttcgtcgagg gtgggtatga ttttcatacc ctctgattta
      721 tcaacgtttt ccctattctc tctatcaaat ttttcgagta agtagatatc attttctgag
      781 acttttaatt ctgctagata tagtctatta ggattatttt tccctaactg tttgttaaat
      841 cccatctgct tttgtaataa taaaccaaag gattccaact cttttttgat tgctatcgct
      901 ttttgttttt ggcagtttaa tacgctacat agttcatttg tcgtataagt aaaataaaca
      961 ttaccttctt catcgatttg ccgtttgctg attgcgatct ctaatcttgc ttttaaaagc
     1021 atataggcaa tcttagttga atctttcatt tttttgtatt cgtcagaaac aataaagact
     1081 tttggaaatt gataaaataa ttcgttgtaa actctatccg ccttaaaaaa attaaaatca
     1141 gtcattgtag atcctctact ttcacgttgt tttatttatg taaaattacc tactcttttc
     1201 gaaaatagca aagataaata aaaaatgcaa acgcattaaa actgtttttt aattaaatac
     1261 tacctaaaat cagaaataaa atga
//
LOCUS       DAZKAO010000248         1276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000248 DAZKAO010000000
VERSION     DAZKAO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.258"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACG8H8_002852"
     CDS             35..532
                     /locus_tag="ACG8H8_002852"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987847.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACG8H8_002853"
     CDS             complement(569..1252)
                     /locus_tag="ACG8H8_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP8987848.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAZKAO010000249         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000249 DAZKAO010000000
VERSION     DAZKAO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.259"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8H8_002854"
     CDS             complement(31..1203)
                     /locus_tag="ACG8H8_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP8987849.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAZKAO010000250         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000250 DAZKAO010000000
VERSION     DAZKAO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.261"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..506
                     /locus_tag="ACG8H8_002855"
     CDS             <1..506
                     /locus_tag="ACG8H8_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HFP8987850.1"
                     /translation="DGLMADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGH
                     KNFSGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSS
                     GGNDSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYS
                     FVITYSI"
     gene            520..951
                     /locus_tag="ACG8H8_002856"
     CDS             520..951
                     /locus_tag="ACG8H8_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987851.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            953..1090
                     /locus_tag="ACG8H8_002857"
     CDS             953..1090
                     /locus_tag="ACG8H8_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP8987852.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          455 a          179 c          268 g          349 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtcaatag accgttctac tttgaacagt gctcaactat
      421 ctattggcgc agacggagcc attaaatgga gaagaaattc aagttatgga tcagcttatt
      481 catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt ttacaaatca
      541 atcaagcctt acggattcga gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac
      601 tgtacagaga ttaaaccacc agttcctaac tggagaccaa gatttgattt tgataaaaaa
      661 cagtgggttg agttagctac tgaagaagag aaaaaaggga atgctgttga tagcgtagat
      721 gaattggcta atattaaggc attatacgaa acgctgaagg cagaaaatga tgaattgaaa
      781 cagctaaatt ctaaagcaat gctaaacaat gtagcaatca agcaagaaaa tgtcttattg
      841 aaagaaaagt cagaaagttt agcgcagttg aattcaaaaa cgatgcttgc ttctgtacaa
      901 aataccaagg aaatcgaaga aatcaaaaaa caattacaag gtgggaagta acatgtattc
      961 atatgaagat atcaaactaa tgtatgactg gggctgtttc actaacgaac aagtaatggt
     1021 tttcgttccg ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag
     1081 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     1141 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     1201 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa g
//
LOCUS       DAZKAO010000251         1250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000251 DAZKAO010000000
VERSION     DAZKAO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.262"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..563
                     /locus_tag="ACG8H8_002858"
     CDS             <1..563
                     /locus_tag="ACG8H8_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFP8987853.1"
                     /translation="AVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            563..1183
                     /locus_tag="ACG8H8_002859"
     CDS             563..1183
                     /locus_tag="ACG8H8_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987854.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          443 a          183 c          295 g          329 t
ORIGIN      
        1 atgctgtttc aaaaacagaa ggagaaatgg gttcttattg gcaatcaatc gaagatttaa
       61 ttgcggatat gacggacttt attaatgaaa ataaaggcga ttttacggac tggatgaatg
      121 aaaggaaaga agaatttgaa cgttggagac aagaacagga acacagtttc caagattgga
      181 gagaaggaca agaatcagac tatttggcat ggtttgaatc aatcaaggat attttaaaga
      241 cgattgatcc aggtggagtc atgttagccg aactaacgga tgcgcgagta gatattcaag
      301 gagttcgtca tgcgtctatt tcagagcgtt tgctggcaga tatggactat ttatatcaga
      361 aattgcgagc aacgcttttc acgattgaat acggggagat tgaagtgact gatattttgc
      421 aggatgatct cttttcagat aatcatgaag tcgaaaaagt tggaactgta gcattcccga
      481 ttgaagaagg agccttgatc atcgcaaccg ttgatgatcc aaagcaaaat gtgttcactc
      541 ttgagaaagt tggggtgatc taatggctaa aactaaacga atgatggaaa cggatgaaaa
      601 aacgggtgta caacgccaat tctttccaat cacacatgct tccgcagttc ttgggttaga
      661 agaaataata gcaggagaag caacagtttt atctgttaat ggaaaaattg gagccgtcgt
      721 cattactaaa gaagatttgg gattagagaa tgttctcaca gaattaccct atgcaagtga
      781 agaagatgac ggcattatca cggcagaaat gtatcaaaaa attttaaaca gtggagaagg
      841 tgactacgtg ttgccagttg ccactatcga ccgtttgggt ggcataaaag ttggtgaatt
      901 attgacgatt gatgagacag ggaaagtctc tgcagtcaga caatctgatg tcaatttttc
      961 gctggagtta aaagaaaaac tcgattcact gaaaaattat actgctggag aaaacatcac
     1021 tattgatgaa gatggaacaa ttagttctac aggtggtagt gggactggtg gtgtcaatca
     1081 gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaaatt acacaaacga
     1141 aagaattcca aattttactt ttgaaaaaat tggggaggta tagagaatga cagatatcgt
     1201 aaaaataaaa caaagcaatg tacaggttta tccacaaact cattggaatg
//
LOCUS       DAZKAO010000252         1247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000252 DAZKAO010000000
VERSION     DAZKAO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.263"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8H8_002860"
     CDS             200..742
                     /locus_tag="ACG8H8_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987855.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1247
                     /locus_tag="ACG8H8_002861"
     CDS             898..>1247
                     /locus_tag="ACG8H8_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP8987856.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DAZKAO010000253         1236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000253 DAZKAO010000000
VERSION     DAZKAO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.264"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..360)
                     /locus_tag="ACG8H8_002862"
     tRNA            complement(287..360)
                     /locus_tag="ACG8H8_002862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Ile,seq:cat)
     gene            complement(915..1229)
                     /locus_tag="ACG8H8_002863"
     CDS             complement(915..1229)
                     /locus_tag="ACG8H8_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987857.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          463 a          194 c          188 g          391 t
ORIGIN      
        1 aaaagagttt aaagcgtaac acacttatgg ggaagtggtt tttggggaac gctttaaact
       61 cttctttatt attatctcac aatataaccc aaatgtcttt ccatttaaaa atcaaagtaa
      121 aacttttcaa atatacagaa gtataactat gtgaaacatc ctttcattaa tccataaaag
      181 gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata
      241 ctcttcaaaa aattctaaca cagaacgatt caattggcta cattaatgta ccctgtagga
      301 ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtag
      361 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
      421 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      481 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      541 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      601 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      661 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      721 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      781 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      841 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      901 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      961 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
     1021 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
     1081 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
     1141 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
     1201 attaagataa aattactgta aaaatccaaa agctat
//
LOCUS       DAZKAO010000254         1235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000254 DAZKAO010000000
VERSION     DAZKAO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.265"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1049
                     /locus_tag="ACG8H8_002864"
     CDS             42..1049
                     /locus_tag="ACG8H8_002864"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP8987858.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1061..>1235
                     /locus_tag="ACG8H8_002865"
     CDS             1061..>1235
                     /locus_tag="ACG8H8_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP8987859.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQ"
BASE COUNT          487 a          159 c          235 g          354 t
ORIGIN      
        1 atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa
       61 tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat
      121 atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac
      181 aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa
      241 ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg
      301 ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa
      361 atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata
      421 aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa
      481 tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa
      541 atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata aaaaacaata
      601 aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg
      661 caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa
      721 tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat aatgatacac
      781 atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat
      841 atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata
      901 tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa
      961 tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg
     1021 caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt
     1081 aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga
     1141 taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct
     1201 agtttccaaa aaggctccaa tggcaaaata tcaga
//
LOCUS       DAZKAO010000255         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000255 DAZKAO010000000
VERSION     DAZKAO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.266"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..346
                     /locus_tag="ACG8H8_002866"
     CDS             <1..346
                     /locus_tag="ACG8H8_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8987860.1"
                     /translation="SFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNGMLVA
                     QRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITELKHA
                     KELREEWFTNDD"
     gene            336..602
                     /locus_tag="ACG8H8_002867"
     CDS             336..602
                     /locus_tag="ACG8H8_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8987861.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            761..1039
                     /locus_tag="ACG8H8_002868"
     CDS             761..1039
                     /locus_tag="ACG8H8_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987862.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1036..>1209)
                     /locus_tag="ACG8H8_002869"
     CDS             complement(1036..>1209)
                     /locus_tag="ACG8H8_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP8987863.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          462 a          168 c          241 g          338 t
ORIGIN      
        1 atcttttgaa acatttaaaa gacccaatgg tcatgatgaa tttttagaat ggttggaaac
       61 attaccaaaa aaagatagtg cgaagctttt acgtacaata gaagaaacag aaaagaatgg
      121 gatgctagta gctcaacggt tgaaatgggt gaaaaaatta gacactaatt tgtacgaatt
      181 aagaagtaag gtaggatcaa acatccaaag agccatctat ttccatgtgg acaatggaag
      241 gtatgtcatt actcacggat ttactaaaaa aacggagaaa acaccaataa ctgaactcaa
      301 gcatgcaaaa gaacttagag aggagtggtt cacaaatgat gattaaaaaa ttgattgaag
      361 aaaagaaaaa agataaagat tttgcagaag catatgaccg tgaaggaaga aaactcgaaa
      421 ctgcggttgc tctttatcat gctcgggagg aagcagggct aactcaagcg gagcttgcac
      481 aaaaagcaca tacaactcaa tctactattg ctcgaattga gagaggggat aacgtatctt
      541 tcgataaact tgatgatatc gccagggcat taggtaaaaa gctggtagta gaatttgctt
      601 aaaagcgagt tgaaaatata tctataatag gctcctaact aatcaattag tttagtgggc
      661 ctttttttac tagatagata aaggttgtac tatagtgtaa aagacaataa attttatact
      721 aaaattactt taaagagaac tagagagaaa ggtacaataa atgaaaacca taaatcttat
      781 aaaagtattt atagcatcac caggtgatat agcagcaaaa aggaatgaag tagaagaaac
      841 catatataga tggaatatag aaaatacaga taatacaaaa gtgattttga tgcctatacg
      901 atgggaaact aatgcgattc ctacttatgt aaataatagc tctgggcaaa gtgttattaa
      961 taaacaaatt gtagaaaata gggttctgct gcaaagtttt aaaaataaaa tcagtttttg
     1021 tattatttct tcattttaag ctggaattcc caataatatc ctgatttcag tgcagactga
     1081 aaagccgaag agagtgcctt cttttcggct tttcttgtag attcctcgaa tggtttccat
     1141 ccctttaatt gtggtggaag ctgtacgtag actttgatac aatttatgtc gacgttttat
     1201 cgatcgatg
//
LOCUS       DAZKAO010000256         1188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000256 DAZKAO010000000
VERSION     DAZKAO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.267"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..>1188)
                     /locus_tag="ACG8H8_002870"
     CDS             complement(174..>1188)
                     /locus_tag="ACG8H8_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP8987864.1"
                     /translation="DFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNLNVAMAD
                     RERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
BASE COUNT          416 a          203 c          135 g          434 t
ORIGIN      
        1 attactactt tgtcatattc atttaacaca ctattattac cattacttag acgctcagat
       61 tctacgaaac aactcaaatc agaaatttcc caaaaaattt tttgttaaaa tccaatttcc
      121 tcaactcctc caaactacgt aaatgatata gtctcataat aaaataaaac ctctcagcta
      181 atgttgttga aattatccac taaacctaat tgatattgct taggagatac acctgcgtac
      241 tttttgaata ctctcgaaaa gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca
      301 ctgatagtat ggtttgattt caacattatt ttagccgtag aaatttttcg cttagagata
      361 taattataaa tcgtaatatg catttcattc ttaaagcttt ctcttaattt agtttcactt
      421 acagaaaaat aattagctat ttcctttaca gtaattcttt tatagatatt taagccgatg
      481 aattgaacga cagaagaaat taagggagaa agatgctcta tcttagtttt tccgatttcc
      541 ttagtataat aaacaatccc agaatccctc acttttaaga cctcaggtaa cgatacagct
      601 aattcgtttt cttgtatcaa agcatctcta ctttgatacg cttctattat atccattcct
      661 aatgttatag acgtttgtgc taacttttca aaaacaatga tagaataatt tttttcagac
      721 cgaatcgtat ctccactaac acttggaatt attccattac tcagattaaa aaccatgttt
      781 tctaaatttc gaatatctcc atttttaaca tattccagta ttttattttc ataaagaaac
      841 agatatattt ctggatcgta attttgtgat aacagattat ctattttgat ttgctcgata
      901 gttttactta gatttcctgt atattcaatt aacggttcat ggaatagatc ttctattttt
      961 ccagtaaaaa aataatgaac taaaatcaga atatctcgaa tgtctcctaa agaatacaaa
     1021 gtcaaatttt gcaatgaatt atagagtcgt tctctatcag ccattgcaac attcaaattg
     1081 ttcattttta gtttaaaaaa atctttatca atattatttg ctctaaatgg accaaataag
     1141 taatatctgg ttttaaacat gtatagaaga aacatctcat taaaatca
//
LOCUS       DAZKAO010000257         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000257 DAZKAO010000000
VERSION     DAZKAO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.268"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..459
                     /locus_tag="ACG8H8_002871"
     CDS             139..459
                     /locus_tag="ACG8H8_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987865.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8H8_002872"
     CDS             912..1112
                     /locus_tag="ACG8H8_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8987866.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          387 a          190 c          210 g          345 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtc tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtttcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca ccagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt ggtcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa aaaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa ta
//
LOCUS       DAZKAO010000258         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000258 DAZKAO010000000
VERSION     DAZKAO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.269"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..412
                     /locus_tag="ACG8H8_002873"
     CDS             2..412
                     /locus_tag="ACG8H8_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8987867.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            426..782
                     /locus_tag="ACG8H8_002874"
     CDS             426..782
                     /locus_tag="ACG8H8_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8987868.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          387 a          165 c          210 g          344 t
ORIGIN      
        1 aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt
       61 gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag
      121 tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg
      181 gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat
      241 cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag
      301 aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg
      361 tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag
      421 ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac
      481 aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatttaa
      541 gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg
      601 cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac
      661 aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg
      721 atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat
      781 agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac
      841 ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg
      901 ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga
      961 catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat
     1021 agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa
     1081 ctgagttgtt aaaaaagaac cacttg
//
LOCUS       DAZKAO010000259         1105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000259 DAZKAO010000000
VERSION     DAZKAO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.270"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8H8_002875"
     CDS             <1..1087
                     /locus_tag="ACG8H8_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987869.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAZKAO010000260         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000260 DAZKAO010000000
VERSION     DAZKAO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.271"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8H8_002876"
     CDS             <1..969
                     /locus_tag="ACG8H8_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP8987870.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKAO010000261         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000261 DAZKAO010000000
VERSION     DAZKAO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.272"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8H8_002877"
     CDS             <1..963
                     /locus_tag="ACG8H8_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP8987871.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          213 g          288 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZKAO010000262         1041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000262 DAZKAO010000000
VERSION     DAZKAO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.273"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            745..>1041
                     /locus_tag="ACG8H8_002878"
     CDS             745..>1041
                     /locus_tag="ACG8H8_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8987872.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDK"
BASE COUNT          383 a          169 c          125 g          364 t
ORIGIN      
        1 ctttaaacaa acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc
       61 tctctttcct ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc
      121 tgtcaaaata agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt
      181 ccaccgtaaa aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact
      241 atttacatat atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta
      301 tacagataat aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt
      361 tcgttcaaat acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa
      421 ataaaaaata caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga
      481 tttattatac gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta
      541 agaagatata tgaattttct tttctaagta taaagataac tatccattat caactaaaaa
      601 aacatttttg aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat
      661 tcgcagcgca gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa
      721 atcttaatgg aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg
      781 attcgattct ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa
      841 gaaattcaaa aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact
      901 ataaaattta aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa
      961 ttagcaaaaa tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt
     1021 atctttaata aaactgataa a
//
LOCUS       DAZKAO010000263         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000263 DAZKAO010000000
VERSION     DAZKAO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.274"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACG8H8_002879"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACG8H8_002879"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAZKAO010000264         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000264 DAZKAO010000000
VERSION     DAZKAO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.275"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1003
                     /locus_tag="ACG8H8_002880"
     CDS             <1..>1003
                     /locus_tag="ACG8H8_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8987873.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          301 a          209 c          191 g          302 t
ORIGIN      
        1 ggcaagaaga tctctatgat tctcctgaat cgtagcggta ataaacggac ctatcttcgg
       61 ttgaagaaac agcgatacct ctgccgagca tgtaaaaaat attttaccgc tcggacgtat
      121 ctcgttaccc cattctgctt tatttctaag cagattcatt acaagatctt agaagaattg
      181 acagagcgtc aatctataaa agcaattggt aaacattgcg atgtttctgt gacaactgtt
      241 cagcggacat tgtccgcctt gtcatcagat atccgtcaga atcttgagta tttacctcgg
      301 tgtctattat tcgatgagtt tcgttctctt tctacatggc acggtaaatt ttctttttct
      361 tgtatggacg gtgagaccgg gaaactgttt gagatcttac ctagccgccg taaaaaagat
      421 ctcgttgctt ttttcatgcg tttttctctc aaagcgaggt tgggtgtaca ttacatcgta
      481 acggatatga acgctcctta tttctcacta gttaaagagt gttttccaaa tgcacaagtg
      541 atcgtcgatc ggtttcacat tgttcaacat ctgaaccgct tcttcgatgc ggttcgaaaa
      601 cgtgtgatga aaacacttga tcaaaaagac ccaatacaag ctaaacagta tcgccaattg
      661 aaatccttat tcaaattact tttaaaatca gaggatcgtt tagattacaa tacactaacg
      721 aaaagacgca atttcaattg gagactattg actgaaacag aagtagttga ccgccttcta
      781 aagatttctc ctgaacttaa aacagcttat cgttactacc aagacctgct tactgcgtat
      841 caggaaaaag atccagatac ttttttccag ctgcttcgag caatgcctgg agaagttcca
      901 ttggaactgc accatatcaa aaaagccttt ttcaaatacg agaaagggat tcggctggca
      961 ttactgaaga agtattcgaa tgcacgactg gaaaatctcc ata
//
LOCUS       DAZKAO010000265         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000265 DAZKAO010000000
VERSION     DAZKAO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.276"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8H8_002881"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8H8_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKAO010000266         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000266 DAZKAO010000000
VERSION     DAZKAO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.277"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..248)
                     /locus_tag="ACG8H8_002882"
     CDS             complement(87..248)
                     /locus_tag="ACG8H8_002882"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFP8987874.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(572..904)
                     /locus_tag="ACG8H8_002883"
     CDS             complement(572..904)
                     /locus_tag="ACG8H8_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987875.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT          298 a          211 c          125 g          368 t
ORIGIN      
        1 aacagcgaca ataatgacgt cattttttga attgtttgcc tttaaaatga ttcattactc
       61 tatcctctct gtcttttttc tcaattttac actaaaatag attttttgga aaactttgca
      121 acagaaccac ttttcgtaat atcaatgtct ttattcgtat gatttttcgt tttacgatct
      181 acatgatttt gaagtccatt taacgaccca actttatgct tttgtgttcg tgccaccata
      241 taggccataa ctgtgtactt cctttcgtta aaattattgc gagaatcaca agctaaattc
      301 ggaaaaacct ccatgtaagg tataacttac tagacttata ctgcgtaaag tctgtaagct
      361 ctgccgaggg ctatatcggc ttaacagccg atgagaaact ccgtttctct caaacgaaaa
      421 agcgaacgaa aaacgactgc cttcgcattc cttttttacg tccttttttc gttcaaaacc
      481 attggctttt tgcgtcattg ttttcacaat ttttattccg caaaaaacca aaagtgaaaa
      541 gtattgcttt tcacttcaac ccctcctcaa tttagttcac attttctgaa cgaaattgtg
      601 ccataaattg agtcaaaccc ttaacatcat tcgtacgcca atatgcacca ttttcttgcc
      661 aaactaatcc atctgcaaat tttcttagcg atactaaaac ctcatcattt actgaatgat
      721 ttcttttatt tgattccaaa aaatctaatg cttcagcagg tgtcttaaag ccttcctttt
      781 tacaatataa tttcgctaaa gaaatcattt ctttctgtct aatctgtttt cgtttcgctc
      841 taatcttcgc ttcttttgct ttcaaatccg ccatttcttt tgccaatatt tctctattgt
      901 tcattttgat tcctcctaaa aataaggcat atttctagtt taaaatattt attttacgaa
      961 tagaggattt gaacaacgaa tgacacaaaa acaacaaaaa aa
//
LOCUS       DAZKAO010000267          987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000267 DAZKAO010000000
VERSION     DAZKAO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.278"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..979
                     /gene="bsh"
                     /locus_tag="ACG8H8_002884"
     CDS             5..979
                     /gene="bsh"
                     /locus_tag="ACG8H8_002884"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP8987876.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          349 a          127 c          174 g          337 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca
      961 aattaaatat ataaattagt taatgtg
//
LOCUS       DAZKAO010000268          982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000268 DAZKAO010000000
VERSION     DAZKAO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.279"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..590
                     /locus_tag="ACG8H8_002885"
     CDS             <1..590
                     /locus_tag="ACG8H8_002885"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFP8987877.1"
                     /translation="SEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFETT
                     AKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHN
                     KVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHE
                     SAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          376 a          136 c          188 g          282 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aa
//
LOCUS       DAZKAO010000269          971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000269 DAZKAO010000000
VERSION     DAZKAO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.280"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..845)
                     /locus_tag="ACG8H8_002886"
     CDS             complement(<1..845)
                     /locus_tag="ACG8H8_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8987878.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDS"
BASE COUNT          271 a          207 c          160 g          333 t
ORIGIN      
        1 ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc
       61 ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa
      121 agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt
      181 acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata
      241 ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa
      301 acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata
      361 tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact
      421 actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct
      481 acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt
      541 gcattgttac caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta
      601 aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga
      661 ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct
      721 tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg
      781 gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg actaattacg
      841 ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt
      901 attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttgg
      961 ctctataatt t
//
LOCUS       DAZKAO010000270          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000270 DAZKAO010000000
VERSION     DAZKAO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.282"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8H8_002887"
     rRNA            <1..>943
                     /locus_tag="ACG8H8_002887"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKAO010000271          906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000271 DAZKAO010000000
VERSION     DAZKAO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.283"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8H8_002888"
     CDS             complement(<1..210)
                     /locus_tag="ACG8H8_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8987879.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="ACG8H8_002889"
     CDS             complement(309..623)
                     /locus_tag="ACG8H8_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987880.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..>906)
                     /locus_tag="ACG8H8_002890"
     CDS             complement(691..>906)
                     /locus_tag="ACG8H8_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987881.1"
                     /translation="ENPIRPQQWSIENKDGEELYEYDMILDNPEYIESGKEETVNYYY
                     ELPIERQDYQIVVRRENGYIVGLPFTL"
BASE COUNT          293 a          179 c           98 g          336 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttc
//
LOCUS       DAZKAO010000272          882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000272 DAZKAO010000000
VERSION     DAZKAO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.284"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            666..>882
                     /locus_tag="ACG8H8_002891"
     CDS             666..>882
                     /locus_tag="ACG8H8_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFP8987882.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPL"
BASE COUNT          304 a          135 c          156 g          287 t
ORIGIN      
        1 aattaatttc caacacaaaa aagagaacaa acgtttgcat gttgctgttt tttagttata
       61 ataatacgcg aagagaggta atttcatttt ttaaaaaagc taaaaacctt gatattactt
      121 cttttcaggc aatacgaata ttattcgctt acattttttt gtataaattt agttgtttta
      181 aatttaattg tctgctacgc ttgtgatgaa ttaattttcg ttctgcactt ttggacgata
      241 aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctggta
      301 accttcgtcg acgtccacag gtggcattaa gacaaccttt aatcccttaa tgcgtttacc
      361 tttcagtgtt acggtattac ccgcaacgga tttagtataa cagatattca aaagtgatac
      421 cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa
      481 gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga
      541 gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct
      601 ctatttttga aaaataacaa gaaaaactta ttaaaaatat ttaaagtaag tgggtggatt
      661 attttatgtc atatacagga attttgagtc tcgaagatat ttgtcactac ggtaaacggt
      721 gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc
      781 agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacgctg
      841 gtaaacaaaa gcaaataatt ttaaaagatc ctatcccttt aa
//
LOCUS       DAZKAO010000273          872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000273 DAZKAO010000000
VERSION     DAZKAO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.285"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACG8H8_002892"
     CDS             <1..>872
                     /locus_tag="ACG8H8_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8987883.1"
                     /translation="SLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQ
                     SYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVI
                     YLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYS
                     YISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVS
                     IDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIV
                     NILLNMIFIPRLGI"
BASE COUNT          267 a          104 c          150 g          351 t
ORIGIN      
        1 tcgttaattg tgttcttatt aatagggaac atatattttt tgttttcaaa tgcttttttt
       61 gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt
      121 agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct
      181 tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt
      241 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
      301 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
      361 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
      421 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
      481 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
      541 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
      601 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
      661 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
      721 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
      781 aaaaaaacat cgtttattgc gattggtaca gtagcaagtg ctatagttaa tatactatta
      841 aacatgattt tcatacctag gttaggaata at
//
LOCUS       DAZKAO010000274          871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000274 DAZKAO010000000
VERSION     DAZKAO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.286"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..359
                     /locus_tag="ACG8H8_002893"
     CDS             42..359
                     /locus_tag="ACG8H8_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP8987884.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(450..815)
                     /locus_tag="ACG8H8_002894"
     CDS             complement(450..815)
                     /locus_tag="ACG8H8_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987885.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          320 a          104 c          151 g          296 t
ORIGIN      
        1 taattttttc ggtatttaat atttaattag gaggtaatat tttgcttttt tatagtaatt
       61 ttatcttaat agtagcgata ttattacttc tgaatatatg gatttttgat aggagtagaa
      121 attcaagtat aggttttaga actaaacgaa gtttgtcttc taaaaaaaat tgggtatact
      181 cacaaactat tttttatgga ggaattgttt taattagttt actttcttca acactttatt
      241 ctttgaatat tatagatgtt tccacctcaa attcaatatc tattattgga atcattattg
      301 ctgcaattat tacacaactg tttttagtgt ttggagagaa gaaaagaagt aaaaaataga
      361 aaaactagtg atagttttgg tgatataaga aaaacatata tgtataaaaa gaagaaagta
      421 gcttctcttt gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag
      481 gctaccgccc ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag
      541 atcagctggg tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa
      601 aataaatagg aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg
      661 ttcctttgaa aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt
      721 acggaatgat aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa
      781 agcaacggaa ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagtgtt
      841 attattttgt tagttttatt gtaaattgaa t
//
LOCUS       DAZKAO010000275          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000275 DAZKAO010000000
VERSION     DAZKAO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.288"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..721
                     /locus_tag="ACG8H8_002895"
     CDS             29..721
                     /locus_tag="ACG8H8_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8987886.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          369 a          130 c          146 g          180 t
ORIGIN      
        1 aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg gtgggattgt
       61 cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg aaattatttt
      121 gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc ttgatcatac
      181 attgacagat aaagagcaag cgattacaga tcttaccgct cgactagcac aaaaagaaca
      241 gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa atgaattaga
      301 aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat tagtcagcaa
      361 ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg agatcaacca
      421 aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac aagaagcaca
      481 agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa tcgcaacact
      541 gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga aattagacga
      601 agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa aagcaaaaga
      661 tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt tgcgtaaata
      721 atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta tactaagtag
      781 ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggac
//
LOCUS       DAZKAO010000276          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000276 DAZKAO010000000
VERSION     DAZKAO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.289"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..231)
                     /locus_tag="ACG8H8_002896"
     CDS             complement(<1..231)
                     /locus_tag="ACG8H8_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987887.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTN"
     gene            complement(233..>821)
                     /locus_tag="ACG8H8_002897"
     CDS             complement(233..>821)
                     /locus_tag="ACG8H8_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP8987888.1"
                     /translation="YAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNS
                     EISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGN
                     MHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFID
                     EMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          303 a          144 c           99 g          275 t
ORIGIN      
        1 gttagttaac aagttaattt tacgtttaaa ggaagaaccg attttccggt gattcacaaa
       61 aacaacatca ttaaatagcc ctaattcttt tatacgctca ctatatcttc tagcgttttc
      121 aaattcagaa taaataaata aatctccttt tgattctcgg taaaaatttt gataaacatt
      181 taatatatta atcaaatgta aaggagtcca agcaatgaaa gctgtcttca tattaattct
      241 ccttttttaa atacaagtaa gaaatgcttc ccaaataata ccagaaaaca attgaaatga
      301 catcgcgctt attgaaaagt acatgagcct ccaccatttc atctataaaa atagatagta
      361 taaaaattaa aacaatacca agaaacgcat aattcacttt acttctaaaa aatgttttga
      421 gtaaactaaa gatatttaac acatagaaag catagataca tataaaagca attataccac
      481 ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt tttagttcat
      541 tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta ttcccataga
      601 aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc gcagctttcc
      661 aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt aatattactt
      721 ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct acttttctat
      781 caatcgatac aggaattgtt gctaaaaaat ccttagcata a
//
LOCUS       DAZKAO010000277          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000277 DAZKAO010000000
VERSION     DAZKAO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.290"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            577..>810
                     /locus_tag="ACG8H8_002898"
     CDS             577..>810
                     /locus_tag="ACG8H8_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987889.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDV"
BASE COUNT          282 a          108 c          143 g          277 t
ORIGIN      
        1 atgaaactag gcttttaaca ataattaggc aaaagtcgct catcctctac aggtgggtga
       61 ttaatgccgc tcgatatggt tgttaaccgt cggttactcg aagattgata tataagactt
      121 agtacctttt tattttgctc catgttatgg ccgaaggtct aaagatacaa tcattagtct
      181 agagaaaaat taagaaccgt aggaactacg gggatcgctt ggtgaataag agacactgct
      241 attagtgaaa aaataagata tcaagtacgc tctattccca aaaatttcgt gactttactc
      301 atgagaggtt caagtagtat atttttaatc aaaattgacg taacttttaa taaatactac
      361 ctaaataagg tagtatttat ttttgtgttt taaaagagga agtccaatat atctatggga
      421 aacttcttaa atattatgtt ggtacatgaa aatatacatc cctgctagac tatttacaac
      481 attttacttt gtacgtattc ttgttttaaa aggaatggga taaaatatag gtacaataac
      541 tatttgatgc taaatataat taatggagga gagatagtga acatacttaa tgaaaaaaga
      601 tatgtatggt ttgctggtaa aaaatatatt actactttca aaagattatc cgagttaatg
      661 cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt ttaattttgt tcaatcttca
      721 gtatctactg aaagtatcta tttttcaatt ctttctgaga gtaggctttt tcattttaga
      781 ataagttctc atgtaaagaa aactgatgtt
//
LOCUS       DAZKAO010000278          799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000278 DAZKAO010000000
VERSION     DAZKAO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.291"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG8H8_002899"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG8H8_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          287 a          125 c          127 g          260 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      781 ctttattcta tgctttata
//
LOCUS       DAZKAO010000279          798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000279 DAZKAO010000000
VERSION     DAZKAO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.292"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACG8H8_002900"
     CDS             <1..197
                     /locus_tag="ACG8H8_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP8987890.1"
                     /translation="NNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGIPA"
     gene            280..>798
                     /locus_tag="ACG8H8_002901"
     CDS             280..>798
                     /locus_tag="ACG8H8_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987891.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMR"
BASE COUNT          279 a          104 c          158 g          257 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaagcg acggaataaa ttttatcgaa
       61 gtctccggac tgcctcatcc acgattaagg ggatggagac cattcgagga atatataaaa
      121 agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgagatc aaggtattaa
      181 tgggaatacc agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac cattatttta aagtgggagg aaaattgttt tgtggatatt taaacaagaa
      301 atctttgcta aattatttag tttagatgga ttaggttggg gaatcttatt ttttattttt
      361 ttatttttag gatctatgtg gatctggttt gttgataaaa aaaattctta tgagaaagta
      421 atagtgaaaa aaatgaatcg aaatgaattc gatgattttt caaaggaaca tagagttgaa
      481 ttgtacgata gtcatgaaat taacagtact tctccatctt attattgggt atttggttgc
      541 gaagaaaaac cattggagat taaattgttg aaaatgcttg aaaatcctcc agtcattgaa
      601 gatttttctg caaaaaacgt tgaaaaacag atgtttgaac aggtacaaat aagaggtcaa
      661 aacaaaattt cattagaacc agatgaatat gcagcctttt ttttagaaac aaattcaggt
      721 acttcaatgc ctgttttcaa agttaaatta aaaatgaatg gtcgtttatg cacttatgat
      781 cttgttgcaa atatgcgc
//
LOCUS       DAZKAO010000280          780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000280 DAZKAO010000000
VERSION     DAZKAO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.293"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..720
                     /locus_tag="ACG8H8_002902"
                     /pseudo
     CDS             10..720
                     /locus_tag="ACG8H8_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
BASE COUNT          259 a          144 c          157 g          220 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc ttaccgctgg
      301 tttttaggct actccttcga ggagaaaatc ccccatttct caacttttgg aaagaattat
      361 gttcgccgat ttagagaaac gacggtgttt gaggatattt ttgcctatat tttagaacaa
      421 gcagttaaag caggttttgt aacagaggat aatttgtatc tggattctac ccacatcaaa
      481 gctaacgcca ataaacataa gtttacgaaa gaaatgaccc atgatgaagc gaaagcctat
      541 caggatgaat tagaagatga gatcaatcgt caacgaatag aggctggaaa acgccctttt
      601 actttggact tagaaaagga agtgaagctg aaagaaagaa aaataagtaa agctgatccg
      661 gaaagcggct actacgtcaa aggcgaacga gaaaaacaat ttgcttattc tgtccatact
      721 taatgcgacg acaatggatt tattcttagc acaatcatca ctcctagaaa tattcatgac
//
LOCUS       DAZKAO010000281          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000281 DAZKAO010000000
VERSION     DAZKAO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.294"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..>770)
                     /locus_tag="ACG8H8_002903"
     CDS             complement(228..>770)
                     /locus_tag="ACG8H8_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8987892.1"
                     /translation="LDLQEDSLNKFGCEKIFTDHMSGAKSNRPGLEMAIDFVRSGDTL
                     VVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSSTGQLMFHLFAAFAEF
                     ERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSGTPIKTIAERWNVSR
                     TTIYRYLAKMEEKNGKTNSH"
BASE COUNT          252 a          166 c          110 g          242 t
ORIGIN      
        1 tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg agagactgca
       61 aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg aataaaatcc
      121 ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca tactattgtc
      181 acttttctag ataaatggca aaaattttca gcattaaatg acaaaaacta atgagaattt
      241 gtctttccat ttttctcctc catttttgcc aaataacgat aaatggtggt tcttgagacg
      301 ttccaccgtt ccgcaatagt cttaataggt gtaccgctat ccactaaagt cttcagtaat
      361 tccaaatctt gttcagacaa tttttcagga cgaccaccaa accgtcctct tgcacgagca
      421 gctgctcgtc ctgctgcgga tcgttccagg atcaaattac gctcaaattc tgcaaatgcc
      481 gcaaacaaat gaaacatgag ctgtcctgta gaacttgatt tgtccatcgt aatattttct
      541 tgaatactat gaaaactcac accacgctca ttcagtcgat tgacgattga aatcaaatct
      601 tccatatttc tacctaaacg atctaagcgc caaacgacaa gtgtatcgcc agagcgaaca
      661 aaatcaatgg ccatttctaa tcctggtcga ttactttttg ccccactcat atgatcagta
      721 aaaatttttt cacaaccaaa tttatttagg ctgtcttctt gtaaatccaa
//
LOCUS       DAZKAO010000282          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000282 DAZKAO010000000
VERSION     DAZKAO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.295"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..383
                     /locus_tag="ACG8H8_002904"
     CDS             69..383
                     /locus_tag="ACG8H8_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987893.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            376..>757
                     /locus_tag="ACG8H8_002905"
     CDS             376..>757
                     /locus_tag="ACG8H8_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987894.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIES"
BASE COUNT          309 a          105 c          133 g          210 t
ORIGIN      
        1 gggtaaatct ctgagggagc aagctcccta cagactccct cccaaaaaaa gaaaggtgga
       61 agaaaaaaat gaactatcca tattttaaag tctcggcttc agaagaaact aaagaaatat
      121 tcaacaattt ttacaaccaa aacaaaggag tttttggttc caaggcaaat atgtttcgtg
      181 ttatggtttc aaatttacca gtgttagcta gtccatcaaa taataaattt aatgattctg
      241 aaagcattaa atttgaacaa aaaatttcag aactagaatc aatgataagt aatgaagtaa
      301 ttgaaaagct tgatgacatt gatcaaaagt tatcgtattc tctaaaaaac aaatacaaaa
      361 cggaggagaa aaaagatgtt taaagtttac ttcccgtttc agtggctcgt taaaggaatt
      421 cttttagtgc tactaggatt gattcttttt ggaagttatc taacaattaa agccagacat
      481 tttccagaag aaaagccaat aaaaattgta acaaaagatt taaaaatagg tgaaacttat
      541 caggcagatg gggttgaact aacatttaaa aatattgaag tgaccgaaaa aagaaatgaa
      601 tttgcacaac atgatcgtgt attacaagtg acctatacac ttaaaaatga tacagaaaaa
      661 ccaatacgtc cacaacaatg gtcgattgaa aataaggatg gtgaagagct ttatgagtat
      721 gctatgatat tagataatcc agagtatatt gaaagtg
//
LOCUS       DAZKAO010000283          738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000283 DAZKAO010000000
VERSION     DAZKAO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.296"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            390..>738
                     /locus_tag="ACG8H8_002906"
     CDS             390..>738
                     /locus_tag="ACG8H8_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8987895.1"
                     /translation="MKILIVDDEASILDIVEAYLSAKGYQVFRALSGNEALTKVDVVQ
                     PDLIVLDLMLPGLSGLEVCKKIRDSSTVPIIMLTAKTAEKDILEGLSLGADDYITKPF
                     SPKELVVRVETVLR"
BASE COUNT          228 a          135 c          170 g          205 t
ORIGIN      
        1 ctttccttta aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt
       61 atttgatagt agatttaaaa ctttgcaaca gaaccagaaa tgaatattgt aaaaaatgat
      121 taggtagcgt ttattaatcc atgttcataa cttaagactt tagacaacat cccaaaagca
      181 gaagattcta ctacgcctga caaagtgatg acctttagtg gcatgggaaa catggtcgcc
      241 attgacggca aacagttcga catggaccgg atcgatttac gcgctaaggt cggggaaaag
      301 gaagtttggg agattaaaaa cacacgggac atgatgggtc aagttttagt agaatgaaga
      361 taccaacgaa attggaggag aaagattacg tgaagatttt aattgtggac gacgaagcaa
      421 gcattctgga tatcgtggaa gcctatttga gtgcaaaagg ctatcaagtg tttcgcgctt
      481 tgagcggaaa cgaggcttta acaaaggttg atgttgtcca acctgatttg attgtgttgg
      541 atctcatgct gcctggttta tctggcctcg aagtctgtaa aaaaataagg gactcctcca
      601 ctgtaccgat tatcatgttg actgcgaaaa cagccgaaaa agatatcttg gaagggttaa
      661 gcctgggggc tgatgactac atcacaaaac cattcagccc gaaagagctg gttgttcggg
      721 tcgaaaccgt tttacgcc
//
LOCUS       DAZKAO010000284          733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000284 DAZKAO010000000
VERSION     DAZKAO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.297"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8H8_002907"
     CDS             complement(<1..446)
                     /locus_tag="ACG8H8_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987896.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          227 a          126 c           95 g          285 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca
      721 ataataaaaa ttt
//
LOCUS       DAZKAO010000285          727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000285 DAZKAO010000000
VERSION     DAZKAO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.298"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..380
                     /locus_tag="ACG8H8_002908"
     CDS             <1..380
                     /locus_tag="ACG8H8_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987897.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
     gene            394..>727
                     /locus_tag="ACG8H8_002909"
     CDS             394..>727
                     /locus_tag="ACG8H8_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987898.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLD"
BASE COUNT          266 a           99 c          133 g          229 t
ORIGIN      
        1 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
       61 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      121 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      181 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      241 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      301 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      361 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      421 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      481 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      541 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      601 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
      661 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga ttacgatgtt
      721 ttggata
//
LOCUS       DAZKAO010000286          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000286 DAZKAO010000000
VERSION     DAZKAO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.299"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..701
                     /locus_tag="ACG8H8_002910"
     CDS             <1..701
                     /locus_tag="ACG8H8_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987899.1"
                     /translation="FKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQ
                     MIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIK
                     TTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSL
                     RAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKN
                     MQNQLVEKLGKIEI"
BASE COUNT          303 a          101 c           94 g          214 t
ORIGIN      
        1 tttttaaatt cgccaaccgt cgatatggat taaaatataa tcccgtaaat ggggtttctt
       61 tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga aaaagatggt tatttatcaa
      121 aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc acatcaacag tcacgttatt
      181 ctttaattac taaatttttg tttttaactg gactacgtta tggtgaattg atatctttga
      241 ctgaatctga ttatgatggt aataaaatta cggtttctgg tacttatgat tacgagttaa
      301 aaattaaaac aacaactaaa aatacaggtt cttatagaac cattgaatta tcaagtaatg
      361 ctaaagaaat catagatcaa ttaattgaag aaaacaaatt aattaagaat aataaagata
      421 aatacatttt catctctaaa aatggtaacc cgatatctat tcaagcatat aatcaatctt
      481 taagagcagt atccaaagaa ttaaacctag ataaaaaagt ttcttctcac atgttaaggc
      541 attcccacat ttctctactt acagaattag gaatcccatt aaaagccatt atggatcgtg
      601 taggacatga agattcaaaa acaacattaa agatttatac tcatacaact aaaaatatgc
      661 aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta aattgcccct tt
//
LOCUS       DAZKAO010000287          696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000287 DAZKAO010000000
VERSION     DAZKAO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.302"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG8H8_002911"
     CDS             complement(54..629)
                     /locus_tag="ACG8H8_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP8987900.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZKAO010000288          692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000288 DAZKAO010000000
VERSION     DAZKAO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.303"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>692
                     /locus_tag="ACG8H8_002912"
     CDS             <1..>692
                     /locus_tag="ACG8H8_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP8987901.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          248 a          118 c          123 g          203 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggacataa agacggcaag aaatctctcg aaattcgaaa atggacttgc
      601 cctatttgtc atactcatca tgaccgagat atcaacgcta gtatcaatat cctgaccgaa
      661 ggtctaaggc tacactctat gggattggct ta
//
LOCUS       DAZKAO010000289          673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000289 DAZKAO010000000
VERSION     DAZKAO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.305"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8H8_002913"
     CDS             85..624
                     /locus_tag="ACG8H8_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987902.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          248 a           86 c          126 g          213 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aat
//
LOCUS       DAZKAO010000290          666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000290 DAZKAO010000000
VERSION     DAZKAO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.306"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..636)
                     /locus_tag="ACG8H8_002914"
     CDS             complement(82..636)
                     /locus_tag="ACG8H8_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8987903.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          198 a          141 c           68 g          259 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgag
//
LOCUS       DAZKAO010000291          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000291 DAZKAO010000000
VERSION     DAZKAO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.308"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..143
                     /locus_tag="ACG8H8_002915"
     CDS             <1..143
                     /locus_tag="ACG8H8_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFP8987904.1"
                     /translation="ELTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQ
                     YV"
     gene            273..>642
                     /locus_tag="ACG8H8_002916"
     CDS             273..>642
                     /locus_tag="ACG8H8_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFP8987905.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSA"
BASE COUNT          275 a           92 c          100 g          175 t
ORIGIN      
        1 acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc
       61 agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt
      121 cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa
      181 aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa
      241 aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc
      301 caggaaaaac cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta
      361 taaatttagt taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag
      421 ccgtacaatt aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg
      481 gaaacgaaag tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta
      541 caaaaaaata ttggaatgaa atggaaaatt taaaaaaata tatagagaag caacaatcta
      601 tattagataa taattccaaa gaaacacaaa tttcaagtgc ag
//
LOCUS       DAZKAO010000292          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000292 DAZKAO010000000
VERSION     DAZKAO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.309"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..506
                     /locus_tag="ACG8H8_002917"
     CDS             <1..506
                     /locus_tag="ACG8H8_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8987906.1"
                     /translation="NVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGV
                     GKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYY
                     LVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTP
                     PRSVIEK"
BASE COUNT          230 a          116 c          108 g          171 t
ORIGIN      
        1 ttaatgttgt tttaagtgtt ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag
       61 aattagataa agccatcgaa caagtaattg ttgtcattcc agaatatcaa tgcttaacca
      121 gtattccagg cgttggtaaa gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg
      181 aaagatttga agatcagaca aaattggcaa aatacgctgg attaagttgg aaagtgaacc
      241 aatctggaaa ctatcagtcg caaaatacac ctctgacaaa acaaggaaat cgatatttta
      301 gatactactt agttgaagct gccaactctg taaaaaatta tcttcctgaa tacaaagctt
      361 tttatcaaag taaatataag gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa
      421 ccgcaagaaa atttacgcgt ctggtggata cgctactacg taaacaccaa ctctatacgc
      481 caccaaggag tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc
      541 cgaaaaaaat ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat
      601 aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZKAO010000293          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000293 DAZKAO010000000
VERSION     DAZKAO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.312"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8H8_002918"
     tRNA            complement(65..150)
                     /locus_tag="ACG8H8_002918"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8H8_002919"
     tRNA            complement(167..238)
                     /locus_tag="ACG8H8_002919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8H8_002920"
     tRNA            complement(250..322)
                     /locus_tag="ACG8H8_002920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8H8_002921"
     tRNA            complement(340..421)
                     /locus_tag="ACG8H8_002921"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8H8_002922"
     tRNA            complement(429..501)
                     /locus_tag="ACG8H8_002922"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8H8_002923"
     tRNA            complement(505..577)
                     /locus_tag="ACG8H8_002923"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZKAO010000294          577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000294 DAZKAO010000000
VERSION     DAZKAO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.313"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          207 a          103 c           95 g          172 t
ORIGIN      
        1 tagaaagaca tttgggttaa attgtgagat aataataaag aagagtttaa agcgcacccc
       61 aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
      121 agcataccat atttttgaaa aaaagtgaga aaaaaggctt acaattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat ctcctttcac aagtatggtg ataaaattcg
      301 ttccgggcta cctttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttta tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcatg
      421 aatcggctac actagactag acagaaaaaa taaggtgtgt agaataataa aaaacacact
      481 ggaggatttt tcatgtcaag acgtcaacga agaacctatt caaaagaatt caaacaacaa
      541 atcgtcgatc tctatctcgc tggtaagcct cgcgcag
//
LOCUS       DAZKAO010000295          575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000295 DAZKAO010000000
VERSION     DAZKAO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.314"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..>575
                     /locus_tag="ACG8H8_002924"
     CDS             79..>575
                     /locus_tag="ACG8H8_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP8987907.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          216 a          106 c           93 g          160 t
ORIGIN      
        1 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
       61 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      121 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      181 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      241 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      301 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      361 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      421 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      481 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      541 gataagtatg tcgctatttt tatggacgct actta
//
LOCUS       DAZKAO010000296          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000296 DAZKAO010000000
VERSION     DAZKAO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.315"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..300
                     /locus_tag="ACG8H8_002925"
     CDS             103..300
                     /locus_tag="ACG8H8_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP8987908.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            302..>572
                     /locus_tag="ACG8H8_002926"
     CDS             302..>572
                     /locus_tag="ACG8H8_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8987909.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGIN"
BASE COUNT          223 a           55 c          105 g          189 t
ORIGIN      
        1 ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt aatgaatgtt
       61 agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca tttaaaaatt
      121 ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg aaaatattat
      181 ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa ggcaactact
      241 tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg gaagtgctaa
      301 aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat ctataagtaa
      361 agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa aattagaaaa
      421 acaaaaatat gaattagatc ccagtccttt aataaataga ttggtgaatt atctatattt
      481 tactgcttat actaataaaa taagattcac tgaatatcaa gaggaattaa taagaaattt
      541 gagtgaaatt ggaagaactg ctggaataaa tg
//
LOCUS       DAZKAO010000297          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000297 DAZKAO010000000
VERSION     DAZKAO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.318"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8H8_002927"
     CDS             <1..390
                     /locus_tag="ACG8H8_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987910.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          205 a           95 c           83 g          166 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccatcctaaa acataagcta tttgtttact tctgtagaat
      481 atatactttg ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg
      541 ctagttaat
//
LOCUS       DAZKAO010000298          534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000298 DAZKAO010000000
VERSION     DAZKAO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.320"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG8H8_002928"
     CDS             <1..>534
                     /locus_tag="ACG8H8_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP8987911.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZKAO010000299          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000299 DAZKAO010000000
VERSION     DAZKAO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.321"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..99
                     /locus_tag="ACG8H8_002929"
     CDS             <1..99
                     /locus_tag="ACG8H8_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP8987912.1"
                     /translation="ELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            100..>521
                     /locus_tag="ACG8H8_002930"
     CDS             100..>521
                     /locus_tag="ACG8H8_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP8987913.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFS"
BASE COUNT          199 a           63 c           97 g          162 t
ORIGIN      
        1 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
       61 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
      121 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
      181 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
      241 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
      301 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
      361 attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat atttgattta
      421 attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatcttga agaattaata
      481 gatgaacatc taaaaaattt agataaagac gtctactttt c
//
LOCUS       DAZKAO010000300          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000300 DAZKAO010000000
VERSION     DAZKAO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.322"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..195)
                     /locus_tag="ACG8H8_002931"
     tRNA            complement(124..195)
                     /locus_tag="ACG8H8_002931"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Glu,seq:ttc)
     gene            complement(207..280)
                     /locus_tag="ACG8H8_002932"
     tRNA            complement(207..280)
                     /locus_tag="ACG8H8_002932"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Ile,seq:gat)
     gene            complement(293..363)
                     /locus_tag="ACG8H8_002933"
     tRNA            complement(293..363)
                     /locus_tag="ACG8H8_002933"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(329..331),aa:Gly,seq:tcc)
     gene            complement(368..440)
                     /locus_tag="ACG8H8_002934"
     tRNA            complement(368..440)
                     /locus_tag="ACG8H8_002934"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Phe,seq:gaa)
     gene            complement(444..516)
                     /locus_tag="ACG8H8_002935"
     tRNA            complement(444..516)
                     /locus_tag="ACG8H8_002935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Asp,seq:gtc)
BASE COUNT          161 a          137 c          110 g          109 t
ORIGIN      
        1 aagttttatt caaatttgtc ttcgccaaca caatttgacg aagagcctaa agataactct
       61 gctaagcttt ttaaaatcat tctttcagat aaaaagaaaa agatactcaa aaagtatctt
      121 ccatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
      181 cttgaccaac gggccaataa aataaatggg cctaaatgga ctcgaaccat cgacctcacg
      241 cttatcaggc gtgcgctcta accagctgag ctataggccc ataaaaacta aaagcgggtg
      301 acgagaatcg aactcgcgac aacagcttgg aaggctgtgg ttttaccact aaactacacc
      361 cgcataatgg cgcgggacag aatcgaactg ccgacacatg gagcttcaat ccattgctct
      421 accaactgag ctaccgagcc aaacggttcc gacgggaatc gaacccgcga tctcctgcgt
      481 gacaggcagg catgttaacc cctacaccac ggaacca
//
LOCUS       DAZKAO010000301          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000301 DAZKAO010000000
VERSION     DAZKAO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.323"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8H8_002936"
     CDS             complement(<1..132)
                     /locus_tag="ACG8H8_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8987914.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKAO010000302          509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000302 DAZKAO010000000
VERSION     DAZKAO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.324"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DAZKAO010000303          502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000303 DAZKAO010000000
VERSION     DAZKAO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.325"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(271..>502)
                     /locus_tag="ACG8H8_002937"
     CDS             complement(271..>502)
                     /locus_tag="ACG8H8_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987915.1"
                     /translation="LNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFRFVYESKS
                     KTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
BASE COUNT          184 a           64 c           88 g          166 t
ORIGIN      
        1 taatgaaaaa cgagaaggaa gatgacttta aagtagttta gtatgaaggt cgattgataa
       61 aacgatgtgg attaatgcta aataatgagt aatttttcaa taatacaaaa actgtgtaca
      121 gtattagaag tagagaaacg ctagcgtcga aaaaaagata tttttcgtga aaagtaatag
      181 gggctaaaaa gaaagtatac aatttattta tgtttctaag catataaaaa agagcgttga
      241 aaggaatatt tccccttcaa cgcttctttt ttagaagatt ttaatgacca tctcaacttc
      301 attttcaggt ttaggtaatt catggaagct tgtgtttatc gtgcttaatt gatcaactaa
      361 ccatgtttta ctttttgact cgtagacaaa tctaaaatta atatcattgt tctcgtgttg
      421 aagaggtttc tccaaataat ctggtgaata ttcatagtca acgtaacctg ctaatacaaa
      481 agaaagcagt ccattattta gt
//
LOCUS       DAZKAO010000304          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000304 DAZKAO010000000
VERSION     DAZKAO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.326"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /locus_tag="ACG8H8_002938"
     CDS             <1..325
                     /locus_tag="ACG8H8_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987916.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          197 a           66 c           90 g          141 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta taat
//
LOCUS       DAZKAO010000305          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000305 DAZKAO010000000
VERSION     DAZKAO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.327"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>488
                     /locus_tag="ACG8H8_002939"
     CDS             <1..>488
                     /locus_tag="ACG8H8_002939"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8987917.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IE"
BASE COUNT          202 a           45 c           87 g          154 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaa
//
LOCUS       DAZKAO010000306          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000306 DAZKAO010000000
VERSION     DAZKAO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.328"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           96 c           56 g          172 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aat
//
LOCUS       DAZKAO010000307          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000307 DAZKAO010000000
VERSION     DAZKAO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.329"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>470
                     /locus_tag="ACG8H8_002940"
     CDS             <1..>470
                     /locus_tag="ACG8H8_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFP8987918.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DAZKAO010000308          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000308 DAZKAO010000000
VERSION     DAZKAO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.330"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..324
                     /locus_tag="ACG8H8_002941"
     CDS             <1..324
                     /locus_tag="ACG8H8_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987919.1"
                     /translation="RENVQSSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKS
                     AKKFDSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRG
                     GLSCN"
BASE COUNT          204 a           55 c           86 g          115 t
ORIGIN      
        1 cgagaaaatg tacaaagttc tacaagtagt caaggattca aaacagtaga tgttcgggag
       61 aacattcaaa aagatagtgg agaaatgaaa gaaaaaacac aaaaaatcaa tatcgttgaa
      121 gacagaaagt cagcgaaaaa atttgattca aatcatgaaa cagtgattat tgatagtgaa
      181 atccgtgaaa atgataaggg atcaaaatca agacgaagat tcacttatga agacaatgag
      241 ttattcagag atacacttaa tgatcctaat ccacttttcg ataatttatt aagaaataaa
      301 agggggggat tgtcatgcaa ttaattctaa agattagtac gcatgatttc ttagaacttg
      361 acgaattatg tgaatcagta aagaacgcaa caaatgaaaa aatcacggtc actaattata
      421 ttgaactgat gattcaagaa gaattaaaga tgaaaaatga
//
LOCUS       DAZKAO010000309          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000309 DAZKAO010000000
VERSION     DAZKAO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.331"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8H8_002942"
     tRNA            complement(20..91)
                     /locus_tag="ACG8H8_002942"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8H8_002943"
     tRNA            complement(113..200)
                     /locus_tag="ACG8H8_002943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8H8_002944"
     tRNA            complement(225..298)
                     /locus_tag="ACG8H8_002944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8H8_002945"
     tRNA            complement(312..382)
                     /locus_tag="ACG8H8_002945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8H8_002946"
     tRNA            complement(384..459)
                     /locus_tag="ACG8H8_002946"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKAO010000310          454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000310 DAZKAO010000000
VERSION     DAZKAO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.332"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>454)
                     /locus_tag="ACG8H8_002947"
     CDS             complement(37..>454)
                     /locus_tag="ACG8H8_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987920.1"
                     /translation="KRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAA
                     YNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDW
                     GNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           83 c           75 g          159 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg tttt
//
LOCUS       DAZKAO010000311          452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000311 DAZKAO010000000
VERSION     DAZKAO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.333"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          173 a           57 c           63 g          159 t
ORIGIN      
        1 ttaaataaag tactaccttt aataggtagt actttattat caagccatta ttccttcttc
       61 tactagttct ttttgtcaca taattttgta atcaataaat gttgcgcaat aagtataaga
      121 attccatttt ctaaatttga aaggtgatcc ataatttcat gttcaagtag tttattaatt
      181 gtccacttgt cgcttcatct aaaaatttta taggttttag gaatgctatt cgcttatttt
      241 aggaagattt taactgtttg ctatagatat atatcatact agtatcgtaa taaaagtact
      301 ctaaaagaag aattttcaaa tacgcaaaat taagtacgaa agagttgaaa caaatttgga
      361 gaaaaatgag catagaatgt ctgatgagtt aaatagacct aaaaattatc taaaagtgga
      421 gcaaaattag gaattacaaa caaaacacta aa
//
LOCUS       DAZKAO010000312          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000312 DAZKAO010000000
VERSION     DAZKAO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.335"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>434
                     /locus_tag="ACG8H8_002948"
     CDS             <1..>434
                     /locus_tag="ACG8H8_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP8987921.1"
                     /translation="NHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          150 a           70 c           86 g          128 t
ORIGIN      
        1 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
       61 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
      121 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
      181 tggaaaaaga aaaataaaaa atccttttat tcatggaaaa tggatgaaac gtacatcaaa
      241 attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt aaccttggat
      301 atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa gcggttagtg
      361 aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat tacaagtgcc
      421 tttaagaaac taaa
//
LOCUS       DAZKAO010000313          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000313 DAZKAO010000000
VERSION     DAZKAO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.336"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>427)
                     /locus_tag="ACG8H8_002949"
     CDS             complement(<1..>427)
                     /locus_tag="ACG8H8_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987922.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAZKAO010000314          409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000314 DAZKAO010000000
VERSION     DAZKAO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.337"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>409)
                     /locus_tag="ACG8H8_002950"
     CDS             complement(<1..>409)
                     /locus_tag="ACG8H8_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987923.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEIN"
BASE COUNT          151 a           67 c           53 g          138 t
ORIGIN      
        1 tattaatttc atgtgcagca cgtaatgcat tccctggagt tgtcaaagag aaaattaatt
       61 ctgctaagct tattattgaa aatactgata acttataatt atatcgtcca ttgacaatca
      121 aatataaact aactatactt gttagaacaa aaaaacaggc gttgacttgt tcttgattag
      181 ttaaaaatat caataaagga atacttaccc aatatatttt tctatttatt tcttctcctc
      241 ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa tgctactggc cactgatagt
      301 tcaaggtggt tgctctccaa ccggtttccc ccattactgt caatggtatt aaacagtata
      361 tagagcaaac caataataaa tttttaacat ttattttctc aaaaacata
//
LOCUS       DAZKAO010000315          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000315 DAZKAO010000000
VERSION     DAZKAO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.338"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>407
                     /locus_tag="ACG8H8_002951"
     CDS             <1..>407
                     /locus_tag="ACG8H8_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8987924.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT          102 a           87 c          107 g          111 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac
      361 tttgctggca gctgtccatt tcatggcaac tgtttagaag ggatggc
//
LOCUS       DAZKAO010000316          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000316 DAZKAO010000000
VERSION     DAZKAO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.339"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..>397)
                     /locus_tag="ACG8H8_002952"
     CDS             complement(112..>397)
                     /locus_tag="ACG8H8_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987925.1"
                     /translation="AVSRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTV
                     KELNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          124 a           74 c           54 g          145 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagca
//
LOCUS       DAZKAO010000317          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000317 DAZKAO010000000
VERSION     DAZKAO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.340"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>391)
                     /locus_tag="ACG8H8_002953"
     CDS             complement(<1..>391)
                     /locus_tag="ACG8H8_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987926.1"
                     /translation="LISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGA
                     MFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFL
                     LLFANSKFRKSIREVLPVKIKWMVSILL"
BASE COUNT          160 a           60 c           50 g          121 t
ORIGIN      
        1 taataatatg cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct
       61 aaattttgaa ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg
      121 ggcacctcta gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata
      181 aaaaacacga atcgtttttg ttatttttga aattaataca ctaattaaac ttaaaataat
      241 agaagtatat ccaaacatag ctccaacatt tggacttgta tagacaccaa ataaccgatg
      301 ttcaataaaa ccttgtcttg caagtaatcc tggcgttcct gttgggacat gataagaaat
      361 caaaaaaata aattgataga tagatattag c
//
LOCUS       DAZKAO010000318          388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000318 DAZKAO010000000
VERSION     DAZKAO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.342"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>388
                     /locus_tag="ACG8H8_002954"
     CDS             <1..>388
                     /locus_tag="ACG8H8_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP8987927.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHE"
BASE COUNT          136 a           73 c           63 g          116 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa tccatgaa
//
LOCUS       DAZKAO010000319          381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000319 DAZKAO010000000
VERSION     DAZKAO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.343"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG8H8_002955"
     tRNA            complement(21..94)
                     /locus_tag="ACG8H8_002955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG8H8_002956"
     tRNA            complement(108..181)
                     /locus_tag="ACG8H8_002956"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG8H8_002957"
     tRNA            complement(209..281)
                     /locus_tag="ACG8H8_002957"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG8H8_002958"
     tRNA            complement(301..374)
                     /locus_tag="ACG8H8_002958"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAZKAO010000320          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000320 DAZKAO010000000
VERSION     DAZKAO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.344"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>370
                     /locus_tag="ACG8H8_002959"
     CDS             82..>370
                     /locus_tag="ACG8H8_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP8987928.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          132 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa
//
LOCUS       DAZKAO010000321          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000321 DAZKAO010000000
VERSION     DAZKAO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.345"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..221)
                     /locus_tag="ACG8H8_002960"
     CDS             complement(60..221)
                     /locus_tag="ACG8H8_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP8987929.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          118 a           82 c           42 g          126 t
ORIGIN      
        1 tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac tactcctttc
       61 tattttctta ctccataact aattgcatcc actggacaag catttttaca tcgtccacat
      121 ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg gcaaactttt
      181 ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac tagactagac
      241 agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc atgccaagac
      301 gtcaacgaag aacctattca aaagaattca aacaacaaat cgtcgatctc tatctcgctg
      361 gtaagcct
//
LOCUS       DAZKAO010000322          366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000322 DAZKAO010000000
VERSION     DAZKAO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.346"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..336
                     /locus_tag="ACG8H8_002961"
     CDS             145..336
                     /locus_tag="ACG8H8_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987930.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          140 a           54 c           60 g          112 t
ORIGIN      
        1 atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata
       61 actaattttg tatgcttctt tgctcgtcct cctccttccc caaaaaataa atagattata
      121 agttcatggg aagaaggtaa atttatgaat actttaaaag aagatttcat tttagcaaag
      181 aatggaaatg aagaagcagt tgtggccatt ctcaaaagat ttagcgcttt ggtccataag
      241 caatcctgga gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata
      301 tatctagcta tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat
      361 aagtaa
//
LOCUS       DAZKAO010000323          334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000323 DAZKAO010000000
VERSION     DAZKAO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.347"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          124 a           55 c           59 g           96 t
ORIGIN      
        1 cttaagatta atgacactaa attaacagac tatacgtagt atctatttgc tatgaccata
       61 tcttatatcc ataagtcaac tcagccggac taaaagggct aatcttacta gaacacaagc
      121 tgcacttgat atgcaaatta accctgaatc ggaaaggtgc ttaaattgga tattaacgga
      181 gggataaagt aaaatttatc tctagggaat gtttagaaag gatccaagtt ccttaacaag
      241 tgacaattag taatgtagta acaacgatag gttgtatgcc gaacatttca atttgatacg
      301 tataaaagac gatttcaagc tcatccgtga taaa
//
LOCUS       DAZKAO010000324          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000324 DAZKAO010000000
VERSION     DAZKAO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.349"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>292
                     /locus_tag="ACG8H8_002962"
     CDS             <1..>292
                     /locus_tag="ACG8H8_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987931.1"
                     /translation="YSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEK
                     SQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVT"
BASE COUNT          112 a           44 c           59 g           77 t
ORIGIN      
        1 ttattctcct atcgcttcat tggagaataa ggagcatata ataaaacacg aagcaaagat
       61 tggcagtcaa tgggtaaacc ttccttataa aacaactggt agttctatca tatatagagt
      121 aactaatgag aagagccaag taatcacact tcctgtatat gcttatttag gaacgaatgt
      181 agaaataaat catcaacctg taaaggagca ggtgaatgct aatggattgg tacaaataag
      241 cggagtcaaa ggagttgctg atattaaagt aagcacaagt tatacagtaa cg
//
LOCUS       DAZKAO010000325          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000325 DAZKAO010000000
VERSION     DAZKAO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.350"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG8H8_002963"
     CDS             <1..278
                     /locus_tag="ACG8H8_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987932.1"
                     /translation="TAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYT
                     TSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          116 a           56 c           50 g           69 t
ORIGIN      
        1 acacagcaag agaagtggaa caatggctta aaaccaacaa aataaggcat tcttatagta
       61 gaaaaggaac accttatgat aatgctggaa tcgaatcttt ccatgcctca ttgaaaaaag
      121 aagaagtcta cacgactagc tactcagatt ttgaagaagc aaatcgagcg ctatttagct
      181 acattgaagg attttataac cgaaatcgaa tccatagctc gattcactat ctaaccccac
      241 aggaatttga agaattagca aaagaaaaaa tggcttaacc gtctctacta a
//
LOCUS       DAZKAO010000326          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000326 DAZKAO010000000
VERSION     DAZKAO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.351"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8H8_002964"
     CDS             <1..>291
                     /locus_tag="ACG8H8_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987933.1"
                     /translation="GIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVP
                     KNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          100 a           62 c           52 g           77 t
ORIGIN      
        1 cggcatccac cataaaagtg ccgtcatcac actcgttgag cgactttcaa aagccattat
       61 cgccttgaaa ccagaaggcc gtaaggcagt tgatattgaa aattcgatta atgaatggct
      121 tcaatccgta cccaaaaatc ttttcaaatc aattaccttt gattgtggaa aagaattctc
      181 taattggaaa agtattagta atacgaatga tattgatatt tatttcgcag acccaggaac
      241 gccttcccaa cggggattaa atgaacattc aaacggactc ctccgaaaag a
//
LOCUS       DAZKAO010000327          275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000327 DAZKAO010000000
VERSION     DAZKAO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.354"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>275
                     /locus_tag="ACG8H8_002965"
     CDS             <1..>275
                     /locus_tag="ACG8H8_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987934.1"
                     /translation="FICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMD
                     MNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTS"
BASE COUNT           80 a           65 c           48 g           82 t
ORIGIN      
        1 tttatttgtg ttgatgggac aaccaatcag ctatttacga tccttgaaga tcgcagactt
       61 tacaaactaa ctcaatactt cctgcgcttt cctagaaagg cccgtttaaa ggtcaagtac
      121 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
      181 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      241 cgtgtccaga tcatgaatcg ttttcgaacc tcaca
//
LOCUS       DAZKAO010000328          272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000328 DAZKAO010000000
VERSION     DAZKAO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.355"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           73 a           42 c           50 g          107 t
ORIGIN      
        1 aatatttaat atgaaaatat gctgaattaa tcaaaactcg agtgttactg ttgttattta
       61 ttgttgttct attgtgtata aaattgtata ggttgtcata ttttcttggt ttagtgaact
      121 actcaccact tagctaaacc taacggtttc ttacgtttga agtgggagct tcttgggaag
      181 agagcatact tgatagcgta ttactttact aacagaggtg cctcttattt accaagctat
      241 ccccgtagtt cctacggttc ttgtttattt ga
//
LOCUS       DAZKAO010000329          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000329 DAZKAO010000000
VERSION     DAZKAO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.358"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>264)
                     /locus_tag="ACG8H8_002966"
     CDS             complement(34..>264)
                     /locus_tag="ACG8H8_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987935.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           77 a           39 c           47 g          101 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaa
//
LOCUS       DAZKAO010000330          261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000330 DAZKAO010000000
VERSION     DAZKAO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.360"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACG8H8_002967"
     CDS             <1..>261
                     /locus_tag="ACG8H8_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987936.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DAZKAO010000331          255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000331 DAZKAO010000000
VERSION     DAZKAO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.361"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>255
                     /locus_tag="ACG8H8_002968"
     CDS             <1..>255
                     /locus_tag="ACG8H8_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP8987937.1"
                     /translation="VNQFELLAKFDDQNRSYYERKIEILREGYEYHYDDEIWSDLSEP
                     LPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT           98 a           30 c           43 g           84 t
ORIGIN      
        1 tagtaaatca atttgagtta ttggctaaat ttgatgacca aaacagatct tattatgaaa
       61 gaaaaatcga aatattgaga gaaggttacg agtatcatta tgatgatgaa atttggagtg
      121 atctttctga gccattgccg aaagaagatt ctcgatttgt attagatgta ttgaacatgt
      181 atcgagatat caattttagt aaatcaaagc taaacttaga tgaaaaccaa attactaatt
      241 catactatac tcatt
//
LOCUS       DAZKAO010000332          253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000332 DAZKAO010000000
VERSION     DAZKAO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.362"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG8H8_002969"
     CDS             <1..>253
                     /locus_tag="ACG8H8_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP8987938.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZKAO010000333          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000333 DAZKAO010000000
VERSION     DAZKAO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.363"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG8H8_002970"
     CDS             complement(32..>252)
                     /locus_tag="ACG8H8_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP8987939.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZKAO010000334          242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000334 DAZKAO010000000
VERSION     DAZKAO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.364"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>242
                     /locus_tag="ACG8H8_002971"
     CDS             <1..>242
                     /locus_tag="ACG8H8_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP8987940.1"
                     /translation="RQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRDT
                     QAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQ"
BASE COUNT           77 a           50 c           50 g           65 t
ORIGIN      
        1 atagacaatc cttctattca tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt
       61 ggcattatct ttatcgtgca attgatgcgg acggcttaac cttagatatc tggttacgaa
      121 agaaacggga tacgcaagca gcctatgctt tcttaaaacg actccataaa cagtttggtg
      181 agccgaaagc aattgtgacc gataaagcac cttctcttgg ctccgccttt agaaagttac
      241 ag
//
LOCUS       DAZKAO010000335          237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000335 DAZKAO010000000
VERSION     DAZKAO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.366"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACG8H8_002972"
     CDS             complement(<1..216)
                     /locus_tag="ACG8H8_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987941.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DAZKAO010000336          236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000336 DAZKAO010000000
VERSION     DAZKAO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.367"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           87 a           26 c           42 g           81 t
ORIGIN      
        1 attttagcta tattagcttt ttccgaaaat aaacaacctg taactgcaaa aaaagaaaca
       61 gtattctctg aaggcaaaat aaaaactcga tatatattag aggtagagac taagtttaaa
      121 aatagatcgg agtaatggtt atgcttttta atgagacgca attatggttt aaatttgatc
      181 cttcgaatag atttgtcaaa gatttttata aggtgtggga ttcagaagtt ttcttt
//
LOCUS       DAZKAO010000337          230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000337 DAZKAO010000000
VERSION     DAZKAO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.368"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>230)
                     /locus_tag="ACG8H8_002973"
     CDS             complement(<1..>230)
                     /locus_tag="ACG8H8_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987942.1"
                     /translation="ISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNS
                     RSVKGLESRFESILLAYSVLLSRAQRRFEGTL"
BASE COUNT           66 a           43 c           40 g           81 t
ORIGIN      
        1 caaagttcct tcaaaacgtc gttgtgctcg acttaataga acactgtaag ccagtaatat
       61 gctttcaaat ctactctcta gccctttaac agaacgtgag ttgaaatttt gacaacctaa
      121 tttttctaag gaagaaaaaa cagtctcaac tgtttttctt tgttttccaa gtaagctgtg
      181 gtctagtttt tctgatttat ccatgttttt acgtggcgga acggatatag
//
LOCUS       DAZKAO010000338          227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000338 DAZKAO010000000
VERSION     DAZKAO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.370"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..227
                     /locus_tag="ACG8H8_002974"
                     /pseudo
     CDS             1..227
                     /locus_tag="ACG8H8_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAZKAO010000339          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000339 DAZKAO010000000
VERSION     DAZKAO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.371"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8H8_002975"
     CDS             <1..>224
                     /locus_tag="ACG8H8_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP8987943.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKAO010000340          222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000340 DAZKAO010000000
VERSION     DAZKAO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.372"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>222
                     /locus_tag="ACG8H8_002976"
     CDS             <1..>222
                     /locus_tag="ACG8H8_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8987944.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           67 a           40 c           45 g           70 t
ORIGIN      
        1 tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt acgttgaaag
       61 agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta ttgatggttg
      121 atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc gcagatggcg
      181 aaacaggaca attaattgat gtcttgccta cgcgtaaatt gc
//
LOCUS       DAZKAO010000341          218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000341 DAZKAO010000000
VERSION     DAZKAO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.373"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>218
                     /locus_tag="ACG8H8_002977"
     CDS             <1..>218
                     /locus_tag="ACG8H8_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8987945.1"
                     /translation="SCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRS
                     IHDRPDNYPDFNSEFGHLEGDTIVGIHH"
BASE COUNT           72 a           41 c           42 g           63 t
ORIGIN      
        1 tttcatgcgg tatgagaaca ctttatcgtt tattttctaa aggaatattt gatattgaca
       61 cactaccgat gaaaggtaaa agaaaaccca atggccatca ggaaaaacgg ggaaaacaac
      121 agtatcagcg ctcaatccat gatcgacctg ataattatcc tgatttcaat tctgagtttg
      181 gtcaccttga aggtgatacg atcgttggca ttcatcat
//
LOCUS       DAZKAO010000342          211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000342 DAZKAO010000000
VERSION     DAZKAO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.374"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..>211
                     /locus_tag="ACG8H8_002978"
     CDS             55..>211
                     /locus_tag="ACG8H8_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8987946.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSG"
BASE COUNT           84 a           33 c           43 g           51 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt t
//
LOCUS       DAZKAO010000343          210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000343 DAZKAO010000000
VERSION     DAZKAO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.denovo.375"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>210
                     /locus_tag="ACG8H8_002979"
     CDS             22..>210
                     /locus_tag="ACG8H8_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP8987947.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIING"
BASE COUNT           82 a           34 c           35 g           59 t
ORIGIN      
        1 cccaagaaat gaggactaca tatgcgttca tcaaaatata ctgaacatta tacagatact
       61 tttataacat tcaaggaaat tatgagaact gtttctaaca tataccacaa ttgtgtacca
      121 gataagatta aaaaccgaag aaatactgat caactgaagc aacgcgatac agtgattatt
      181 gcttgtgtta tatggggcat aattaatggc
//
LOCUS       DAZKAO010000344        10145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.095, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000344 DAZKAO010000000
VERSION     DAZKAO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.095"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..668
                     /locus_tag="ACG8H8_002980"
     CDS             <1..668
                     /locus_tag="ACG8H8_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP8987948.1"
                     /translation="FWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSF
                     YSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGY
                     LTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPEN
                     IFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSV
                     ENK"
     gene            complement(818..1759)
                     /locus_tag="ACG8H8_002981"
     CDS             complement(818..1759)
                     /locus_tag="ACG8H8_002981"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8987949.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1885..3006
                     /locus_tag="ACG8H8_002982"
     CDS             1885..3006
                     /locus_tag="ACG8H8_002982"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP8987950.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3264..4766
                     /gene="eat(A)"
                     /locus_tag="ACG8H8_002983"
     CDS             3264..4766
                     /gene="eat(A)"
                     /locus_tag="ACG8H8_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP8987951.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4833..5360
                     /locus_tag="ACG8H8_002984"
     CDS             4833..5360
                     /locus_tag="ACG8H8_002984"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8987952.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5571..6632
                     /locus_tag="ACG8H8_002985"
     CDS             5571..6632
                     /locus_tag="ACG8H8_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP8987953.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6635..7531
                     /gene="murQ"
                     /locus_tag="ACG8H8_002986"
     CDS             6635..7531
                     /gene="murQ"
                     /locus_tag="ACG8H8_002986"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP8987954.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7545..9527
                     /locus_tag="ACG8H8_002987"
     CDS             7545..9527
                     /locus_tag="ACG8H8_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP8987955.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9592..10038)
                     /gene="nrdI"
                     /locus_tag="ACG8H8_002988"
     CDS             complement(9592..10038)
                     /gene="nrdI"
                     /locus_tag="ACG8H8_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP8987956.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3307 a         1799 c         2208 g         2831 t
ORIGIN      
        1 ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca caagatgaac
       61 cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt gggctgaaac
      121 ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca aagacggatc
      181 tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat aagcaggaat
      241 ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg aatgaacaac
      301 ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc gggattctgg
      361 aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc atgtattttc
      421 aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat cgtcctgaaa
      481 atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg catacaccat
      541 ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg gcaaaacgat
      601 ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc tctgttgaaa
      661 acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag cacaattctt
      721 atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa aaaagagaac
      781 cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca aggattcctt
      841 cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata tcatattttt
      901 gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga tggtttttga
      961 tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca taagtagaag
     1021 caatatgttg gtacacttct tcttgatcta gtatgattcc ttcttttgct gcgatagaag
     1081 caaattcaga tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg cctagttcag
     1141 caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca caagcttttc
     1201 gccaaatgga ataacgaacg ttatggctat agatcggatt taaatttgct tcttgaaaga
     1261 cttctacaac tttttttgta aatgcttcgc cttctggttc caagttctct agttcgattt
     1321 ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc cacatcgtga
     1381 tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca tgacctaatc
     1441 catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc gaacaaaaca
     1501 tatcatctaa ttgattggct tttgttaatg ctacgatcaa gtccacgtgt tcgttgcttt
     1561 cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta ccgttaaagt
     1621 cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga tcgatcaaga
     1681 ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt gctccggccc
     1741 cagctattgc gattttcatg ttttttctct cctttattca atgagttccc ttttagtata
     1801 tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac
     1861 ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc atttaaagga
     1921 agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga tcgtaaagga
     1981 agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc aatctattac
     2041 gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg tcagcgaaaa
     2101 atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga atctgcacaa
     2161 gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact tgcttccagt
     2221 tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca aattattgtc
     2281 actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc cttgggtgct
     2341 tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact tcattttgat
     2401 cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt taccaatcca
     2461 tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg aatagatacg
     2521 acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa agatttctgg
     2581 gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg tggagccctt
     2641 tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat cactggattg
     2701 gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat cgatcagcaa
     2761 acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata tcgaaaacca
     2821 ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca
     2881 attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa
     2941 acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg
     3001 aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga
     3061 gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg
     3121 tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg
     3181 tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt
     3241 ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct
     3301 acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga
     3361 aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa
     3421 acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac
     3481 agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga
     3541 tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag
     3601 aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg
     3661 atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa
     3721 aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc
     3781 ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac
     3841 tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa
     3901 tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa
     3961 aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg
     4021 atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga
     4081 aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat
     4141 taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc
     4201 gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc
     4261 attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt
     4321 catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc
     4381 agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt
     4441 tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa
     4501 agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac
     4561 ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg
     4621 aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg
     4681 tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg
     4741 agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa ggtatggtta
     4801 aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga cacggagaaa
     4861 caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag
     4921 gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga
     4981 aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt gctgatcgtc
     5041 tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg gattatgatg
     5101 gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct gtacgattcc
     5161 caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg aaagaatgca
     5221 tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata agagtgatca
     5281 atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta gataatacag
     5341 aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat ttcaaggcac
     5401 tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa tataaactca
     5461 acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt ttctttagat
     5521 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
     5581 tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
     5641 catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg
     5701 catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga
     5761 tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc
     5821 ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga
     5881 ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag
     5941 atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt
     6001 attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc
     6061 tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc
     6121 cactatatca aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg
     6181 ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag
     6241 aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
     6301 aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg
     6361 atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag
     6421 caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc
     6481 gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg
     6541 tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga
     6601 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     6661 aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc
     6721 cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac
     6781 aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat
     6841 ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc
     6901 gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat
     6961 gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa
     7021 gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     7081 tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg
     7141 caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc
     7201 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa
     7261 tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga
     7321 tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac
     7381 agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct
     7441 ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga
     7501 agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac
     7561 tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag
     7621 aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa
     7681 atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc
     7741 aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag
     7801 gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat
     7861 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     7921 aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga
     7981 ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     8041 tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct
     8101 tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt
     8161 ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga
     8221 atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg
     8281 ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata
     8341 aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg
     8401 gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg
     8461 gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat
     8521 tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa
     8581 tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac
     8641 aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa
     8701 tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag
     8761 taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg
     8821 cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt
     8881 tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta ttggcagctt
     8941 atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag
     9001 aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa
     9061 cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt gtttctttag
     9121 ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac tttgtagaac
     9181 cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc ttcccaacga
     9241 aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat atgggaatca
     9301 acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg cagcaagtga
     9361 cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca ggcaaagaaa
     9421 catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa
     9481 caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat gatagaaaca
     9541 atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt attatttatt
     9601 gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc gtaattcata
     9661 atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta gacaatactg
     9721 attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat gattgtcttc
     9781 atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt tatctaaacc
     9841 atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt ctttcgtaaa
     9901 atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat cttctgcatg
     9961 cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc ctgaaatcga
    10021 tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtata gcatcttttt
    10081 taagtttttt ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta taatgagaaa
    10141 aaagc
//
LOCUS       DAZKAO010000345         5287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000345 DAZKAO010000000
VERSION     DAZKAO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.150"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8H8_002989"
     CDS             3..188
                     /locus_tag="ACG8H8_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987957.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8H8_002990"
     CDS             203..1072
                     /locus_tag="ACG8H8_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP8987958.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8H8_002991"
     CDS             1053..1787
                     /locus_tag="ACG8H8_002991"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8987959.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8H8_002992"
     CDS             1820..2728
                     /locus_tag="ACG8H8_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP8987960.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="ACG8H8_002993"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="ACG8H8_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP8987961.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="ACG8H8_002994"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="ACG8H8_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP8987962.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4430..4561
                     /locus_tag="ACG8H8_002995"
                     /pseudo
     CDS             4430..4561
                     /locus_tag="ACG8H8_002995"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            4637..5130
                     /locus_tag="ACG8H8_002996"
                     /pseudo
     CDS             4637..5130
                     /locus_tag="ACG8H8_002996"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1821 a          795 c         1186 g         1485 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     4201 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     4261 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     4321 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     4381 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     4441 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     4501 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     4561 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     4621 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     4681 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     4741 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     4801 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     4861 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     4921 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     4981 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     5041 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     5101 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     5161 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
     5221 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
     5281 ttgtgat
//
LOCUS       DAZKAO010000346         4800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.160, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000346 DAZKAO010000000
VERSION     DAZKAO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.160"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..784
                     /gene="vanR-A"
                     /locus_tag="ACG8H8_002997"
     CDS             89..784
                     /gene="vanR-A"
                     /locus_tag="ACG8H8_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP8987963.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            762..1916
                     /locus_tag="ACG8H8_002998"
     CDS             762..1916
                     /locus_tag="ACG8H8_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP8987964.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2131..3099
                     /locus_tag="ACG8H8_002999"
     CDS             2131..3099
                     /locus_tag="ACG8H8_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP8987965.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3092..4123
                     /gene="vanA"
                     /locus_tag="ACG8H8_003000"
     CDS             3092..4123
                     /gene="vanA"
                     /locus_tag="ACG8H8_003000"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP8987966.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4129..4737
                     /locus_tag="ACG8H8_003001"
     CDS             4129..4737
                     /locus_tag="ACG8H8_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP8987967.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1279 t
ORIGIN      
        1 ggccttttat cttttacaca attatatgga ctttatccta taatctgata aattattaag
       61 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      121 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      181 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      241 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      301 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      361 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      421 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      481 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      541 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      601 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      661 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      721 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      781 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
      841 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
      901 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
      961 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1021 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1081 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1141 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1201 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1261 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1321 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1381 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1441 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1501 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1561 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1621 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1681 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1741 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1801 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     1861 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     1921 tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag gaaattaaca
     1981 atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta agaacgagcc
     2041 gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg
     2101 tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga
     2161 tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca
     2221 acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt
     2281 atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc
     2341 ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct
     2401 aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat
     2461 actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa
     2521 aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt
     2581 gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt
     2641 aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag
     2701 ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat
     2761 attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg
     2821 cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc
     2881 ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa
     2941 aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca
     3001 ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa
     3061 aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt
     3121 tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa
     3181 cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa
     3241 aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc
     3301 gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca
     3361 tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg
     3421 tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg
     3481 tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt
     3541 ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt
     3601 taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt
     3661 ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt
     3721 ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga
     3781 ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga
     3841 aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg
     3901 acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg
     3961 tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc
     4021 cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc
     4081 cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga
     4141 tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg
     4201 gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac
     4261 gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg
     4321 cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca
     4381 cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg
     4441 atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt
     4501 acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg
     4561 gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga
     4621 cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac
     4681 tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt taaataaact
     4741 tttaaccgtt gcacggacaa acggttctgt tgcaaagttt taaataaaga ataaaatccc
//
LOCUS       DAZKAO010000347         3640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.186, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000347 DAZKAO010000000
VERSION     DAZKAO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.186"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1178
                     /gene="rlmN"
                     /locus_tag="ACG8H8_003002"
     CDS             111..1178
                     /gene="rlmN"
                     /locus_tag="ACG8H8_003002"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP8987968.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1168..1374
                     /locus_tag="ACG8H8_003003"
     CDS             1168..1374
                     /locus_tag="ACG8H8_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987969.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1371..2474)
                     /locus_tag="ACG8H8_003004"
     CDS             complement(1371..2474)
                     /locus_tag="ACG8H8_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8987970.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3027..3575
                     /gene="aac(6')"
                     /locus_tag="ACG8H8_003005"
     CDS             3027..3575
                     /gene="aac(6')"
                     /locus_tag="ACG8H8_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP8987971.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1197 a          618 c          733 g         1092 t
ORIGIN      
        1 tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat
       61 gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac
      121 agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga
      181 aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca
      241 cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca
      301 atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg
      361 aactccctga taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag
      421 tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc
      481 tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac
      541 attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg
      601 gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca
      661 aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa
      721 tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga
      781 ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga
      841 tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca
      901 tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag
      961 ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc
     1021 aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg
     1081 gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat
     1141 tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca
     1201 attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa
     1261 gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga
     1321 aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt
     1381 ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc
     1441 tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg
     1501 gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat
     1561 actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga
     1621 tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc
     1681 attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta
     1741 ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca
     1801 attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag
     1861 agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca
     1921 tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc
     1981 cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt
     2041 ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc
     2101 gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt
     2161 ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg
     2221 tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta
     2281 cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg
     2341 ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct
     2401 ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa
     2461 acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga
     2521 gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga
     2581 tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta
     2641 caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc
     2701 ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg
     2761 ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg
     2821 aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa
     2881 gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac
     2941 tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga
     3001 ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag
     3061 tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca
     3121 gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag
     3181 accaagatga gttagtagga tttattggtg caatccctca atacggtatc acaggttggg
     3241 aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag
     3301 tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg
     3361 atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata
     3421 aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt
     3481 ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg
     3541 caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa
     3601 tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DAZKAO010000348         3574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000348 DAZKAO010000000
VERSION     DAZKAO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.188"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..421
                     /locus_tag="ACG8H8_003006"
                     /pseudo
     CDS             72..421
                     /locus_tag="ACG8H8_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(693..983)
                     /locus_tag="ACG8H8_003007"
     CDS             complement(693..983)
                     /locus_tag="ACG8H8_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987972.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1385..1621
                     /locus_tag="ACG8H8_003008"
     CDS             1385..1621
                     /locus_tag="ACG8H8_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8987973.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1628..>3574
                     /locus_tag="ACG8H8_003009"
     CDS             1628..>3574
                     /locus_tag="ACG8H8_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP8987974.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYF"
BASE COUNT         1248 a          600 c          648 g         1078 t
ORIGIN      
        1 tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt
       61 taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa
      121 aaaattttca ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat
      181 gcagaattcg tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga
      241 acaagatata gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg
      301 acgttctcgt gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct
      361 cttcgagtta cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg
      421 agactagtaa tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa
      481 tagggcttcg aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag
      541 tttagtgatt catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt
      601 gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg
      661 gctaggacat ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc
      721 ttttttcttg ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca
      781 tgataggatc tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc
      841 atcccttcgt ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc
      901 ccatttaaac tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct
      961 actgttccat attttttctc cataaccttc acctcaatac cattatacga acatacattc
     1021 cctttgtaaa gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa
     1081 aatcatattg gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt
     1141 atagatttaa gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc taaatgagta
     1201 tcaaattgca actgaatttg atactcgttt tttattgtta aatcaacgtt tgttgttaat
     1261 ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa taattattgt
     1321 aggaatgcaa attggtgtgt taaaatggtt acaatttact aagcaaaaag gggtgcattt
     1381 tcctatgcaa tttcttgagc caaaaaataa gaacgctaag tctgtggatt gggaaatatc
     1441 agagcaagta agggtgatag ttaagcaata cgctgaatat gcagagcgta ctgaaagcga
     1501 agcagtggat gaattcttat tgaacatttt agacgataag aagtttattg aatggattgc
     1561 caacaagcga agtaataagc ggattgttga aaaaatgggt atcaaagata gagtgggttg
     1621 atagacaatg accaagctta aaagattagc cacgaaggaa gatgaaattg tagatgttaa
     1681 aattccgatt tcaaacgaag aattaaaaga tagggctaaa caatatgatt tattaactcc
     1741 aaaacgattt gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt ttaaatggaa
     1801 cggttctgaa tatcccattc aatataatta ctgcatcaat ccgttttgtt gtaattttgg
     1861 gaaagaacag cacaaattca aagatgtaaa agggaaacca agtcgatata aaatgactgg
     1921 tagttccaaa gataaaggtc ataaaggtat gtactgcaat gacaatccta ttggcagagg
     1981 tgtatcgcaa aactgtacag taaccccttt atctaattgg tctgttgttg aagaaattaa
     2041 acgccttata gaaatcaata gcatccaaga tgttgagcca gattatcaat ttcataaaga
     2101 aggctgctcg gaagaagaat caaccccatt caatgagccg aagcaatttt acaaaagggg
     2161 gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt tcactaatgt
     2221 tctaccaaaa agagaagaaa ccactactta tcaccaacaa aagaacacca ttcttccaat
     2281 gttcgctaag atggttgttg gaagggtttc agtcagtcgt acttgtgata ttttaggaat
     2341 tggagttgga acctactatc ataagctgga gtggctgtat aggcgttgtt tggagttttt
     2401 agaacgatat gagacacaac cccttcaaac aaagaaattt aacgagatgt ggcttaatac
     2461 ggacaagatg cactactacc taaataacgt ccgtaagaaa ggacaaggtt caaagaaata
     2521 tacggggttt gaagacctta atatgcaaac gtatatagtt gtatctgctg aggtcttatc
     2581 aagatatgtg ttccgttctg atgttgccta tgattggaat atttcaatgg atgaattaaa
     2641 tgaagataca agaaaattca aagaagacca tctcaatact tttagtcgta aaaatgatag
     2701 gttagattgg tcttattatc cacaagaacc atccgctaac gatagcgaaa ataggaatgc
     2761 ttatcttcac gaattaggga aaattactaa taggagtaga tttgtcgatg gtttaaatgt
     2821 tgatgctcct tatactacaa ctgctcacta ttggttaatt aaacaaatgg ttaatgccga
     2881 cgaatggcga atgattagtg atgatgactt ctctattaga aacgcctttt atagagtatt
     2941 cacgaaagaa ttaaggttga gcgatgcaca tcattttatt tgccaagtta acaagacgaa
     3001 aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa gcagaactgc tcgattgggg
     3061 agatatcaga ggattcaaaa caaagtttct acggactttg gcatctcatt atctaactga
     3121 attactaaca agtcatcaat tccacgaaga ggctattagt aaagatggag aaagatatcg
     3181 caaatatgcc gataacccta tcaaacatcc attagccact aaggataaag gattttattc
     3241 agttgattgt agaacggatt tatctgctct ggaaccaaac gaaatcgcaa agatgctatt
     3301 aaatgttaat gaccattcga ccaatagctt tattcaacag ataagacgtt atatatcatc
     3361 tcttgaaaga ccattaacaa cagctcgtgg tgataagaag agctatattt acgctaactt
     3421 caatccgaag tatgctcaat ttgcgattac aattctaaga acttactaca acttttgtcg
     3481 cccttttaaa tcagcggata aaaaggtttt gacacctgct caaagattag ggattacaga
     3541 taaacaattt gattggaaag atataattta tttc
//
LOCUS       DAZKAO010000349         3096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000349 DAZKAO010000000
VERSION     DAZKAO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.195"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8H8_003010"
     CDS             260..1171
                     /locus_tag="ACG8H8_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP8987975.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8H8_003011"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8H8_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP8987976.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8H8_003012"
     CDS             2328..2612
                     /locus_tag="ACG8H8_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8987977.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1146 a          442 c          570 g          938 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctactt
//
LOCUS       DAZKAO010000350         2772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.204, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000350 DAZKAO010000000
VERSION     DAZKAO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.204"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8H8_003013"
     CDS             111..194
                     /locus_tag="ACG8H8_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP8987978.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8H8_003014"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8H8_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP8987979.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG8H8_003015"
     CDS             complement(1319..2182)
                     /locus_tag="ACG8H8_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP8987980.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG8H8_003016"
     CDS             complement(2184..2456)
                     /locus_tag="ACG8H8_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP8987981.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG8H8_003017"
     CDS             complement(2473..2682)
                     /locus_tag="ACG8H8_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP8987982.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZKAO010000351         2417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000351 DAZKAO010000000
VERSION     DAZKAO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.216"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1396
                     /gene="mef(H)"
                     /locus_tag="ACG8H8_003018"
                     /gene_synonym="mefH"
     CDS             158..1396
                     /gene="mef(H)"
                     /locus_tag="ACG8H8_003018"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFP8987983.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1623..2042
                     /locus_tag="ACG8H8_003019"
     CDS             1623..2042
                     /locus_tag="ACG8H8_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP8987984.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2047..2286
                     /locus_tag="ACG8H8_003020"
     CDS             2047..2286
                     /locus_tag="ACG8H8_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8987985.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          767 a          337 c          445 g          868 t
ORIGIN      
        1 acattattag acgaagaaac aatttctgca tttatacagt tttgtaaata actgtatttg
       61 ttatccctat acgatagact gctctataaa agagcagttt atttaaacca ttatataaac
      121 cgacggtctg tttgattaaa taaaaaggag agattttatg caagcagaaa aaaaactttt
      181 taatcgtgat tttatacttg ttgttatagg acagataatt tctttatttg gaaacgctat
      241 cctacgattt gcattaccgt actatttgtt aaaagaaact ggttcatcag ctttgtttgg
      301 tattgctaca gcctgctctt ttttacctat ggttattttg tcgctggtag gtggtattat
      361 tgctgacagg acaaataaaa gaaacattat ggtcatacta gattttgtaa cagcaatcat
      421 aattttagta ttttatttgt cattgggaaa aattcctatc atacctttgt ttattgttgc
      481 tttgatgtta ctttatggta tttctggtgc atatcaacca gcggtacaag caagtattcc
      541 tctattggta tcatcagata aacttaccgt cggtaatgct ataataaatc aagtaaatac
      601 tttagcaaac tttatcggac cagctataag cggagtagtt tttcaatttt atggtatcaa
      661 tcccatttta attgttagta ttatatgttt tactttttca gccattatgg aaatttttat
      721 ccatattcca caccaaaaaa gacctcgtga agtaggtgta attgcagttg ctaaaaacga
      781 tttaaaagat agttatacct ttttgaaaaa ggagaaaccc atttttatat ccgtaatctt
      841 tttggtttgc attttcaatt tggttttaag tgctgtttta attgttggtc tgcctatctt
      901 aatttctgaa gttttaaaaa tgccagatac actatatggt ttttcacaat cagcattatc
      961 attaggtggt ttatgtggtg gtattttaac agctcttgtt gcttcaaagt taaaactaaa
     1021 aaacctttat accttgttac ttatttgttc tgtttctgtt ttacttatgg gaatttcgct
     1081 atttttagaa ttgcctgcaa tagtttccta ctgggtagtt acagtgatgt gctttgtggc
     1141 tatgggtgta tctactatgt ttgttgttca aatttacact cttgttcaga cacaaacacc
     1201 accgcaatta gtaggaaaga taatggctac gctggtagca gttgctatgt gcggtcaacc
     1261 tattggacaa atggtttacg gtattttatt cgatgtattt atacaagaaa catgggttgt
     1321 tatgtttgta gcttctattg cttcaatcat gatttcaata tattcaaaaa gaatttttca
     1381 aaagttagag atataataca aggggttaaa tagtatgtta ggctaaaaac aatatataaa
     1441 agaaaatttg aaatctgcta aaaggatagt gttataaaag aaagagtaat attgcagtat
     1501 ttaaggctcg cacaaatata tgttttgtgt gggctttttt cttaccattt ttctgtattt
     1561 ctaaagggta taaacggtat tccgtttata tatttcagca ttagcagaaa gggggtgaga
     1621 ttatgaggac atcttctttc gagcatatcg tcagattaca gttcaattct ttaatgctga
     1681 ttgtcattaa gaacaaagtg aaaagtcgaa acagacagct tgcaagacat tctaaacgtg
     1741 aagtattgtt ttgtgaaata gccgaaacga aacaagttga acgtggaacg attgatacct
     1801 gtttttatga ttgtgtatca tttaaagtct tgcactttac aatttatgta tctgatgaaa
     1861 cacttggtac agttttacat agactgtcag aaaaacagcg tagtgcgatt ttattacgtt
     1921 attttcaagg aatgaacgac agagaaattt cagaactgta ccatgtatca cgttctgcta
     1981 tttgcagtag aagaactaga ggactaagga agttaaaaat gcttttgagc gaaaggaagt
     2041 aaagaaatga gtaaagatta tggctatccc tcttttgaat tgatttgcaa agcgtcaagc
     2101 ggagatgaaa tggcagttaa agagattttg aagttttatg acggttatat ttctaaatta
     2161 tgcttgcgtc ctttttacca ctctgaaagt ggtaaaatca ttatgcaggt tgatgaagaa
     2221 ctaaaaggag aaattcatac agacatgatg aaagcaatat tgaaatttga aatcggagtg
     2281 aagtaatcaa ctgtataaaa aaatcgggag atacacagtt tcgtgctatc tcccttagta
     2341 gttttacggt catatcaagg ctcattcaat cgtggtaaat tcgtggtaag atgagtgttt
     2401 ttctatactt caaaatg
//
LOCUS       DAZKAO010000352         2068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.229, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000352 DAZKAO010000000
VERSION     DAZKAO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.229"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..589
                     /locus_tag="ACG8H8_003021"
     CDS             <1..589
                     /locus_tag="ACG8H8_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HFP8987986.1"
                     /translation="EIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNV
                     LEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNN
                     RLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIV
                     EEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(719..1891)
                     /locus_tag="ACG8H8_003022"
     CDS             complement(719..1891)
                     /locus_tag="ACG8H8_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFP8987987.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          674 a          291 c          371 g          732 t
ORIGIN      
        1 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
       61 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      121 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      181 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      241 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
      301 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
      361 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
      421 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
      481 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
      541 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
      601 atgaaaagct attataaaag acattagtat taaatagttt aaaaaaatga aaaataataa
      661 aggaagtgag tcaagtccag actcctgtgt aaaatgctat acaatgtttt taccatttct
      721 acttatcaaa attgatgtat tttcttgaag aataaatcca ttcatcatgt aggtccataa
      781 gaacggctcc aattaagcga ttggctgatg tttgattggg gaagatgcga ataatctttt
      841 ctcttctgcg tacttcttga ttcagtcgtt caattagatt ggtactcttt agtcgattgt
      901 gggaatttcc ttgtacggta tattgaaagg cgtcttcgaa tccatcatcc aatgatgcgc
      961 aagcttttga atattttggt tgatcgatat aatcatgaat caatcgattt ttagcctcac
     1021 gcgctaagtt aatatctgtg aacttaaaaa ttcctttaac agcttctctg aaagattttg
     1081 aatttttttt aggaatggtg gtaaagatat ttcttaggaa gtgaacttgg catctttgcc
     1141 aacttacgtt ggtgaaggat tttctaatgg cagagactaa tcctttgtgc gcatcagaaa
     1201 taacgagttc cgtaccttgt aaaccgcgtt cttttaggta ttcaaaaaat gttgtccagg
     1261 tctcttcgct ttcgccactt tgaatcatga agccgataat ttcacggtcg ccatctttgg
     1321 ttattccaat cgctatatga cagctttttg agagtactcg attttcttct cgtactttta
     1381 tatagagtac atcggtcatt aagtaaggat aatttttttc tgataataaa cgattctgcc
     1441 actcgttaac cataggttct agctgttctg ttaagctaga aacgaaggac ttagagacgg
     1501 atttaccaca aagttcttcc acaatttttg atactttacg agttgaaacg cctgatacat
     1561 acatttccaa cattgaagcc atgagggctt tttcgtttcg ttgataacgt tcaaacactg
     1621 tgggtgaaaa atggccatca cgtgttctgg gtacttttaa ttctagcgtg cctacacgtg
     1681 tcgtaaagct gcgctcataa tagccatttc gttgactttg tcggttttct gttcgttcat
     1741 attcttttgc ttgaatatat tctgttcgtt gattttccat tagttgatta aataccgttg
     1801 ttaaaatatt tttagaaacg tcatccttta cagaatattc aataatgctt tgaatctctt
     1861 cgcttttcag tgtaaaatgt acttgggtca tgtaaaagtc ctcctgggta tgtttttgtc
     1921 gttaaaaaca ttgtaccgta aaaggactgt tatatggcct ttttactttt acacaattat
     1981 acggacttta tctatataaa ggcgctgtca gagttttttg acttagaaca tacattcatt
     2041 caagagactt taaacaagct agcagaac
//
LOCUS       DAZKAO010000353         1603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.244, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000353 DAZKAO010000000
VERSION     DAZKAO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.244"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1515
                     /gene="msr(C)"
                     /locus_tag="ACG8H8_003023"
     CDS             37..1515
                     /gene="msr(C)"
                     /locus_tag="ACG8H8_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP8987988.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          576 a          273 c          338 g          416 t
ORIGIN      
        1 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
       61 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
      121 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
      181 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
      241 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
      301 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
      361 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
      421 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
      481 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
      541 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
      601 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
      661 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
      721 gtgtccagta aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa
      781 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
      841 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
      901 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
      961 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     1021 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     1081 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     1141 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     1201 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     1261 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     1321 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     1381 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     1441 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     1501 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     1561 attgcctaca aataaaaaag aaggaataat ttttacaaca atg
//
LOCUS       DAZKAO010000354          699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4550
            SAMEA115707185-rid22570313.guided.301, whole genome shotgun
            sequence.
ACCESSION   DAZKAO010000354 DAZKAO010000000
VERSION     DAZKAO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707185
            Sequence Read Archive: ERR13196916
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287917.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287917.1
            Genome Coverage       :: 173x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:40:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 39 of 92
            Pseudo Genes (incomplete)         :: 47 of 92
            Pseudo Genes (internal stop)      :: 30 of 92
            Pseudo Genes (multiple problems)  :: 20 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707185-rid22570313.guided.301"
                     /isolate="VRE4550"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="ACG8H8_003024"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="ACG8H8_003024"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP8987989.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          266 a           74 c          147 g          212 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcatt
//
